AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069144.t1 MTR_5g031390 95.568 361 16 0 1 361 1 361 0.0 688
MS.gene069144.t1 MTR_5g031310 82.825 361 61 1 1 360 1 361 0.0 602
MS.gene069144.t1 MTR_5g031230 81.440 361 67 0 1 361 1 361 0.0 600
MS.gene069144.t1 MTR_5g031305 81.994 361 64 1 1 360 1 361 0.0 596
MS.gene069144.t1 MTR_5g031250 80.886 361 69 0 1 361 1 361 0.0 588
MS.gene069144.t1 MTR_4g115300 80.609 361 70 0 1 361 1 361 0.0 588
MS.gene069144.t1 MTR_5g031300 78.453 362 77 1 1 361 1 362 0.0 572
MS.gene069144.t1 MTR_5g031210 79.224 361 75 0 1 361 1 361 0.0 571
MS.gene069144.t1 MTR_5g031360 80.057 351 70 0 10 360 12 362 0.0 566
MS.gene069144.t1 MTR_5g031190 75.623 361 88 0 1 361 1 361 0.0 565
MS.gene069144.t1 MTR_5g031180 78.393 361 78 0 1 361 1 361 0.0 549
MS.gene069144.t1 MTR_8g068190 78.889 360 76 0 1 360 1 360 0.0 548
MS.gene069144.t1 MTR_8g092630 63.585 357 124 2 8 359 60 415 1.57e-152 436
MS.gene069144.t1 MTR_5g031430 60.580 345 136 0 14 358 14 358 6.34e-142 407
MS.gene069144.t1 MTR_5g031250 80.498 241 47 0 1 241 1 241 3.00e-135 386
MS.gene069144.t1 MTR_1g025950 57.880 349 146 1 8 355 7 355 1.42e-134 388
MS.gene069144.t1 MTR_1g107425 51.603 343 166 0 11 353 9 351 1.64e-118 347
MS.gene069144.t1 MTR_2g009810 52.450 347 161 3 14 358 62 406 4.29e-115 340
MS.gene069144.t1 MTR_1g107425 52.482 282 133 1 11 292 9 289 5.01e-93 280
MS.gene069144.t1 MTR_2g009820 51.064 235 106 2 130 358 23 254 1.71e-63 203
MS.gene069144.t1 MTR_5g031290 81.579 114 21 0 247 360 80 193 3.70e-60 192
MS.gene069144.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 1.21e-43 150
MS.gene069144.t1 MTR_8g095220 28.755 233 142 7 10 221 51 280 3.65e-19 87.4
MS.gene069144.t1 MTR_8g095220 29.817 218 127 8 25 221 68 280 5.13e-19 88.2
MS.gene069144.t1 MTR_6g466320 25.000 312 210 8 1 300 6 305 3.12e-18 85.1
MS.gene069144.t1 MTR_6g465010 25.000 312 210 8 1 300 6 305 3.12e-18 85.1
MS.gene069144.t1 MTR_6g465010 25.168 298 200 7 15 300 3 289 3.51e-18 85.1
MS.gene069144.t1 MTR_3g089977 24.928 349 223 10 39 354 37 379 1.99e-17 83.2
MS.gene069144.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 4.70e-17 82.0
MS.gene069144.t1 MTR_3g089940 25.074 339 216 9 39 344 37 370 4.74e-17 82.0
MS.gene069144.t1 MTR_3g089970 24.779 339 217 9 39 344 38 371 1.57e-16 80.5
MS.gene069144.t1 MTR_0001s0660 25.434 346 225 12 38 355 35 375 6.03e-15 76.3
MS.gene069144.t1 MTR_5g031380 57.407 54 18 1 80 128 19 72 1.02e-14 70.1
MS.gene069144.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 1.38e-14 74.3
MS.gene069144.t1 MTR_3g089940 25.333 300 194 7 39 312 37 332 1.59e-14 74.3
MS.gene069144.t1 MTR_7g068320 26.934 349 218 14 39 353 48 393 4.29e-13 70.1
MS.gene069144.t1 MTR_5g045710 23.411 299 200 8 38 312 35 328 4.76e-12 67.0
MS.gene069144.t1 MTR_1g069430 25.220 341 216 12 1 312 1 331 6.53e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069144.t1 AT4G37980 70.000 350 105 0 11 360 7 356 5.38e-170 478
MS.gene069144.t1 AT4G37990 71.839 348 98 0 11 358 7 354 3.51e-169 476
MS.gene069144.t1 AT4G39330 61.625 357 137 0 1 357 1 357 1.60e-163 461
MS.gene069144.t1 AT4G37970 61.972 355 135 0 6 360 7 361 5.80e-158 447
MS.gene069144.t1 AT2G21890 60.807 347 135 1 12 357 6 352 1.71e-152 434
MS.gene069144.t1 AT2G21730 61.207 348 133 2 12 357 6 353 4.84e-152 433
MS.gene069144.t1 AT4G39330 61.386 303 117 0 1 303 1 303 4.39e-134 385
MS.gene069144.t1 AT4G37980 68.836 292 91 0 11 302 7 298 9.02e-132 379
MS.gene069144.t1 AT4G37970 59.932 292 117 0 6 297 7 298 1.40e-120 350
MS.gene069144.t1 AT3G19450 52.586 348 164 1 11 357 9 356 1.86e-112 332
MS.gene069144.t1 AT1G72680 50.145 345 167 2 16 358 14 355 2.48e-112 331
MS.gene069144.t1 AT4G34230 51.130 354 172 1 7 359 4 357 5.59e-103 308
MS.gene069144.t1 AT4G34230 50.847 354 173 1 7 359 4 357 4.70e-102 305
MS.gene069144.t1 AT4G37970 58.159 239 100 0 6 244 7 245 1.12e-92 278
MS.gene069144.t1 AT4G37970 58.650 237 98 0 6 242 7 243 1.48e-92 277
MS.gene069144.t1 AT5G63620 32.039 206 115 7 25 209 70 271 9.23e-23 97.8
MS.gene069144.t1 AT5G63620 32.039 206 115 7 25 209 70 271 1.81e-22 98.2
MS.gene069144.t1 AT5G63620 27.402 281 172 9 25 278 70 345 2.11e-22 98.2
MS.gene069144.t1 AT5G51970 26.909 275 178 8 39 300 43 307 4.30e-19 87.8
MS.gene069144.t1 AT5G51970 26.909 275 178 8 39 300 43 307 4.30e-19 87.8
MS.gene069144.t1 AT1G22430 26.899 316 201 12 39 327 42 354 9.99e-17 81.3
MS.gene069144.t1 AT1G22430 26.899 316 201 12 39 327 42 354 9.99e-17 81.3
MS.gene069144.t1 AT1G32780 26.503 366 214 14 39 358 37 393 1.25e-16 80.9
MS.gene069144.t1 AT1G22440 26.821 302 180 12 39 307 40 333 1.58e-15 77.4
MS.gene069144.t1 AT5G43940 25.143 350 217 12 39 352 36 376 1.76e-15 77.4
MS.gene069144.t1 AT5G43940 25.143 350 217 12 39 352 48 388 1.78e-15 77.4
MS.gene069144.t1 AT1G77120 23.529 391 251 13 1 355 1 379 2.97e-14 73.6
MS.gene069144.t1 AT1G64710 25.369 339 210 13 39 340 13 345 4.24e-13 70.1
MS.gene069144.t1 AT1G64710 25.369 339 210 13 39 340 36 368 4.54e-13 70.1
MS.gene069144.t1 AT1G22430 28.877 187 115 5 39 208 42 227 5.87e-13 69.3
MS.gene069144.t1 AT1G22430 28.877 187 115 5 39 208 42 227 8.19e-13 68.6
MS.gene069144.t1 AT1G22430 28.877 187 115 5 39 208 42 227 8.19e-13 68.6

Find 83 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATTTCACATCTGCTTTA+AGG 0.136438 5.3:+20369706 None:intergenic
GCTGGTTCCATCACTATATT+TGG 0.170826 5.3:+20370672 None:intergenic
TTCTTTGGATGCTCAAATTC+AGG 0.236168 5.3:+20371417 None:intergenic
TATCGATTTGTGATTGATAT+TGG 0.243992 5.3:-20369687 MS.gene069144:CDS
GCCCAACCAAATGCCTTCTT+TGG 0.249082 5.3:+20371402 None:intergenic
CGGACGAGCACTTTGTGTTT+TGG 0.259890 5.3:-20370618 MS.gene069144:CDS
AATTAGTACATCACCTAAAA+AGG 0.281212 5.3:-20370413 MS.gene069144:CDS
TCTTGGTGGACTTGGCCATA+TGG 0.337845 5.3:-20370476 MS.gene069144:CDS
TACGAACTATCCACTAGTTC+CGG 0.344610 5.3:-20370957 MS.gene069144:CDS
TTTAGGTGATGTACTAATTA+CGG 0.352263 5.3:+20370417 None:intergenic
GTGAAATTTGCCAAAGCCTT+TGG 0.370310 5.3:-20370451 MS.gene069144:CDS
GTTGTTGGTCTTGGTGGACT+TGG 0.372585 5.3:-20370484 MS.gene069144:CDS
CACATTAGCACCAAAGGCTT+TGG 0.375006 5.3:+20370441 None:intergenic
GAGCATCCAAAGAAGGCATT+TGG 0.380999 5.3:-20371408 MS.gene069144:CDS
ACCATCAGTGAGTGTCAATT+GGG 0.382344 5.3:+20370696 None:intergenic
TATTGTACCTTCATCTGATT+CGG 0.385508 5.3:+20370337 None:intergenic
ATAGGAATGATCTCAATATC+AGG 0.391852 5.3:+20369759 None:intergenic
CATCCTCTATTACCTCTGAT+TGG 0.394736 5.3:-20370070 MS.gene069144:CDS
AACCATCAGTGAGTGTCAAT+TGG 0.401417 5.3:+20370695 None:intergenic
ATAGGTGTTGTTGGTCTTGG+TGG 0.407624 5.3:-20370490 MS.gene069144:CDS
CCCCTTTCAATTTCTCCAGA+AGG 0.415467 5.3:-20371353 MS.gene069144:intron
AATATAGGTGTTGTTGGTCT+TGG 0.416186 5.3:-20370493 MS.gene069144:CDS
GCATCAAGAGGTAACTCATC+TGG 0.417564 5.3:+20370592 None:intergenic
ACGAACTATCCACTAGTTCC+GGG 0.427510 5.3:-20370956 MS.gene069144:CDS
TGGGCAGCTAGAGACTCTTC+TGG 0.441511 5.3:-20371384 MS.gene069144:CDS
TGGCAAATTTCACAGCCATA+TGG 0.446571 5.3:+20370461 None:intergenic
TCCAAAGAAGGCATTTGGTT+GGG 0.449991 5.3:-20371403 MS.gene069144:CDS
TTCATAAGGCGAAGAATGAA+TGG 0.450733 5.3:-20370985 MS.gene069144:CDS
GGTATGAATATAGGTGTTGT+TGG 0.459605 5.3:-20370499 MS.gene069144:CDS
GTAGGGAGAAAGATAGTGGC+TGG 0.463044 5.3:-20369849 MS.gene069144:intron
ATATACAGACATGAAATTGT+TGG 0.463799 5.3:-20370844 MS.gene069144:intron
GAATAATTTGTAGGCTGCAA+TGG 0.469877 5.3:-20370131 MS.gene069144:intron
AACCTTCTGGAGAAATTGAA+AGG 0.475349 5.3:+20371351 None:intergenic
GCTGTGTTGACGTAATCAAT+AGG 0.480301 5.3:+20369741 None:intergenic
ACCTTCTGGAGAAATTGAAA+GGG 0.480545 5.3:+20371352 None:intergenic
GTAGGCTGCAATGGGTACTT+TGG 0.485806 5.3:-20370122 MS.gene069144:intron
TCATAAGGCGAAGAATGAAT+GGG 0.490720 5.3:-20370984 MS.gene069144:CDS
AATAATTTGTAGGCTGCAAT+GGG 0.491959 5.3:-20370130 MS.gene069144:intron
ATTCATTCTTCGCCTTATGA+AGG 0.497579 5.3:+20370987 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.498766 5.3:-20370568 MS.gene069144:CDS
ATGTCACTCGGACCTTCATA+AGG 0.505748 5.3:-20370999 MS.gene069144:CDS
CAGAGTAGGTGTCGGATGCT+TGG 0.505785 5.3:-20370773 MS.gene069144:CDS
ATAGTGGCTGGAAGTTTGAT+TGG 0.509085 5.3:-20369837 MS.gene069144:CDS
GACAAGCCTGGTATGAATAT+AGG 0.509901 5.3:-20370508 MS.gene069144:CDS
ATTGGTTTACTCAAGTCTCA+TGG 0.521108 5.3:-20370052 MS.gene069144:CDS
AGAAAAGCCACCATGAGTGC+TGG 0.530485 5.3:+20370654 None:intergenic
ATCCAAAGAAGGCATTTGGT+TGG 0.531418 5.3:-20371404 MS.gene069144:CDS
ATATATATACCTGCAAGTAA+AGG 0.531437 5.3:+20369977 None:intergenic
TGAATTTGAGCATCCAAAGA+AGG 0.538083 5.3:-20371415 MS.gene069144:CDS
AAAGTGAGCAAGTTCAAAGT+TGG 0.541588 5.3:-20370799 MS.gene069144:CDS
AGTGATGGAACCAGCACTCA+TGG 0.542579 5.3:-20370664 MS.gene069144:CDS
AAAGCTTACCCCGGAACTAG+TGG 0.544688 5.3:+20370947 None:intergenic
GCATTAAAAGTGTTGTATTG+TGG 0.545788 5.3:-20371025 MS.gene069144:CDS
AAACCAATCAGAGGTAATAG+AGG 0.546078 5.3:+20370067 None:intergenic
ATTGGTAGGGAGAAAGATAG+TGG 0.553404 5.3:-20369853 MS.gene069144:intron
GTCTCATGGAAAGCTTGTAA+TGG 0.559201 5.3:-20370038 MS.gene069144:CDS
CCCCAATTGACACTCACTGA+TGG 0.559334 5.3:-20370697 MS.gene069144:CDS
TGGCTGGAAGTTTGATTGGT+GGG 0.560766 5.3:-20369833 MS.gene069144:CDS
TACGGTCACATTAGCACCAA+AGG 0.563794 5.3:+20370435 None:intergenic
GCTGCAATGGGTACTTTGGA+TGG 0.565027 5.3:-20370118 MS.gene069144:CDS
GTTGGCATAGTTACAGAAGT+GGG 0.578876 5.3:-20370826 MS.gene069144:CDS
GTGGCTGGAAGTTTGATTGG+TGG 0.585158 5.3:-20369834 MS.gene069144:CDS
TCGTGATCCGAATCAGATGA+AGG 0.588520 5.3:-20370344 MS.gene069144:intron
TGTTGGCATAGTTACAGAAG+TGG 0.590305 5.3:-20370827 MS.gene069144:CDS
GATGGTTCCAAATATAGTGA+TGG 0.592641 5.3:-20370679 MS.gene069144:CDS
CATGGAAAGCTTGTAATGGT+TGG 0.604281 5.3:-20370034 MS.gene069144:CDS
ATTTCACATCTGCTTTAAGG+AGG 0.611618 5.3:+20369709 None:intergenic
GTAATACCAGCACAAAGGAG+AGG 0.623289 5.3:+20370562 None:intergenic
TGATTACGTCAACACAGCGA+TGG 0.626468 5.3:-20369736 MS.gene069144:CDS
AGAGGAGCAGCAGCATCAAG+AGG 0.635270 5.3:+20370580 None:intergenic
TCGAGTTCAAAATGTCGAAG+AGG 0.636928 5.3:+20370529 None:intergenic
TTCAAAGTTGGAGACAGAGT+AGG 0.639117 5.3:-20370787 MS.gene069144:CDS
TATTATCGACACAGTTTCAG+CGG 0.643532 5.3:-20370095 MS.gene069144:CDS
GTTGGAGACAGAGTAGGTGT+CGG 0.649941 5.3:-20370781 MS.gene069144:CDS
ACAACACCTATATTCATACC+AGG 0.652836 5.3:+20370502 None:intergenic
CCTTCTGGAGAAATTGAAAG+GGG 0.653235 5.3:+20371353 None:intergenic
CCATCAGTGAGTGTCAATTG+GGG 0.658178 5.3:+20370697 None:intergenic
CATAAGGCGAAGAATGAATG+GGG 0.693942 5.3:-20370983 MS.gene069144:CDS
GACTTGAGTAAACCAATCAG+AGG 0.695064 5.3:+20370058 None:intergenic
CGAACTATCCACTAGTTCCG+GGG 0.700828 5.3:-20370955 MS.gene069144:intron
ACACAGTAATACCAGCACAA+AGG 0.710961 5.3:+20370557 None:intergenic
GATGGAACCAGCACTCATGG+TGG 0.728708 5.3:-20370661 MS.gene069144:CDS
GTATTGTGGAATATGTCACT+CGG 0.739362 5.3:-20371011 MS.gene069144:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAATATAAATTGAATA+TGG + chr5.3:20370011-20370030 None:intergenic 10.0%
!!! ATATTTTGATTTATATGTTT+GGG + chr5.3:20370194-20370213 None:intergenic 10.0%
!!! ATTTAATTTAAACATTTAGA+AGG + chr5.3:20371185-20371204 None:intergenic 10.0%
!!! TATATTTTGATTTATATGTT+TGG + chr5.3:20370195-20370214 None:intergenic 10.0%
!!! TTTAATTTAAACATTTAGAA+GGG + chr5.3:20371184-20371203 None:intergenic 10.0%
!! AAATTCAATTTGATTCTAAT+TGG - chr5.3:20370214-20370233 MS.gene069144:intron 15.0%
!! ATATTATGTTTAGAAAGAAA+AGG + chr5.3:20369786-20369805 None:intergenic 15.0%
!! AAATAACATCATGAAAAAAG+TGG - chr5.3:20369921-20369940 MS.gene069144:intron 20.0%
!!! ATCAATCTAGAATAATTTGT+AGG - chr5.3:20370951-20370970 MS.gene069144:intron 20.0%
!!! TATGTTTGGGTTTTTTTAAA+TGG + chr5.3:20370181-20370200 None:intergenic 20.0%
! AAGTATTTCACATTATTGGT+AGG - chr5.3:20371224-20371243 MS.gene069144:intron 25.0%
! AATTAGTACATCACCTAAAA+AGG - chr5.3:20370678-20370697 MS.gene069144:CDS 25.0%
! ACCAATAATGTGAAATACTT+GGG + chr5.3:20371224-20371243 None:intergenic 25.0%
! AGTATTTCACATTATTGGTA+GGG - chr5.3:20371225-20371244 MS.gene069144:intron 25.0%
! ATATACAGACATGAAATTGT+TGG - chr5.3:20370247-20370266 MS.gene069144:intron 25.0%
! ATATATATACCTGCAAGTAA+AGG + chr5.3:20371117-20371136 None:intergenic 25.0%
! ATGCTTTACATTTGTTTGTA+AGG - chr5.3:20369861-20369880 MS.gene069144:intron 25.0%
! ATTACTATGTCAAATTTCCA+GGG - chr5.3:20370023-20370042 MS.gene069144:CDS 25.0%
! CTAATTGTCAACTTCTAAAT+CGG + chr5.3:20370924-20370943 None:intergenic 25.0%
! GAAAAAGTGAGCAAATAAAA+TGG + chr5.3:20369835-20369854 None:intergenic 25.0%
! TACCAATAATGTGAAATACT+TGG + chr5.3:20371225-20371244 None:intergenic 25.0%
! TATAGTTGCTTATTTAGAGA+CGG - chr5.3:20370806-20370825 MS.gene069144:CDS 25.0%
! TATTACTATGTCAAATTTCC+AGG - chr5.3:20370022-20370041 MS.gene069144:CDS 25.0%
! TCATTCACAAAATTACTCAA+CGG - chr5.3:20370898-20370917 MS.gene069144:intron 25.0%
! TCGTCAAAATTAGTAGAAAT+AGG + chr5.3:20369895-20369914 None:intergenic 25.0%
! TTTAGGTGATGTACTAATTA+CGG + chr5.3:20370677-20370696 None:intergenic 25.0%
!!! TATCGATTTGTGATTGATAT+TGG - chr5.3:20371404-20371423 MS.gene069144:CDS 25.0%
AATGAATGAATTGAACCTTC+TGG + chr5.3:20369756-20369775 None:intergenic 30.0%
ATAGGAATGATCTCAATATC+AGG + chr5.3:20371335-20371354 None:intergenic 30.0%
ATGATGATAATCAAACACCT+AGG + chr5.3:20369971-20369990 None:intergenic 30.0%
GATATTTCACATCTGCTTTA+AGG + chr5.3:20371388-20371407 None:intergenic 30.0%
GCATTATCTTATTATGTCCT+AGG - chr5.3:20369951-20369970 MS.gene069144:intron 30.0%
TAACATCATGAAAAAAGTGG+AGG - chr5.3:20369924-20369943 MS.gene069144:intron 30.0%
TACATCACCTAAAAAGGAAA+AGG - chr5.3:20370684-20370703 MS.gene069144:CDS 30.0%
TATTGTACCTTCATCTGATT+CGG + chr5.3:20370757-20370776 None:intergenic 30.0%
TCCCAAGTATTTCACATTAT+TGG - chr5.3:20371220-20371239 MS.gene069144:intron 30.0%
TGAAATACTTGGGAGTTTAA+AGG + chr5.3:20371214-20371233 None:intergenic 30.0%
! AATAATTTGTAGGCTGCAAT+GGG - chr5.3:20370961-20370980 MS.gene069144:CDS 30.0%
! ATTGCTTCCTTTTCCTTTTT+AGG + chr5.3:20370694-20370713 None:intergenic 30.0%
! CTTTTCTAACTCAATGGTTT+CGG - chr5.3:20370453-20370472 MS.gene069144:CDS 30.0%
!! GCATTAAAAGTGTTGTATTG+TGG - chr5.3:20370066-20370085 MS.gene069144:CDS 30.0%
AAACCAATCAGAGGTAATAG+AGG + chr5.3:20371027-20371046 None:intergenic 35.0%
AAAGTGAGCAAGTTCAAAGT+TGG - chr5.3:20370292-20370311 MS.gene069144:intron 35.0%
ACAACACCTATATTCATACC+AGG + chr5.3:20370592-20370611 None:intergenic 35.0%
AGGGCATATTTAGCATCATA+TGG + chr5.3:20371165-20371184 None:intergenic 35.0%
ATTCATTCTTCGCCTTATGA+AGG + chr5.3:20370107-20370126 None:intergenic 35.0%
CATTAGTCATTAGTGACTAG+TGG - chr5.3:20370868-20370887 MS.gene069144:intron 35.0%
GATGGTTCCAAATATAGTGA+TGG - chr5.3:20370412-20370431 MS.gene069144:CDS 35.0%
GTATTGTGGAATATGTCACT+CGG - chr5.3:20370080-20370099 MS.gene069144:CDS 35.0%
TAAAAGTAGAAAGCTTACCC+CGG + chr5.3:20370156-20370175 None:intergenic 35.0%
TATTATCGACACAGTTTCAG+CGG - chr5.3:20370996-20371015 MS.gene069144:CDS 35.0%
TCATAAGGCGAAGAATGAAT+GGG - chr5.3:20370107-20370126 MS.gene069144:CDS 35.0%
TGAATTTGAGCATCCAAAGA+AGG - chr5.3:20369676-20369695 MS.gene069144:CDS 35.0%
TTCATAAGGCGAAGAATGAA+TGG - chr5.3:20370106-20370125 MS.gene069144:CDS 35.0%
TTCTTTGGATGCTCAAATTC+AGG + chr5.3:20369677-20369696 None:intergenic 35.0%
! AACCTTCTGGAGAAATTGAA+AGG + chr5.3:20369743-20369762 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGAAA+GGG + chr5.3:20369742-20369761 None:intergenic 35.0%
! ATTGGTTTACTCAAGTCTCA+TGG - chr5.3:20371039-20371058 MS.gene069144:CDS 35.0%
! GAATAATTTGTAGGCTGCAA+TGG - chr5.3:20370960-20370979 MS.gene069144:CDS 35.0%
! GGTATGAATATAGGTGTTGT+TGG - chr5.3:20370592-20370611 MS.gene069144:CDS 35.0%
!! AATATAGGTGTTGTTGGTCT+TGG - chr5.3:20370598-20370617 MS.gene069144:CDS 35.0%
!! ATTTCACATCTGCTTTAAGG+AGG + chr5.3:20371385-20371404 None:intergenic 35.0%
AACCATCAGTGAGTGTCAAT+TGG + chr5.3:20370399-20370418 None:intergenic 40.0%
ACACAGTAATACCAGCACAA+AGG + chr5.3:20370537-20370556 None:intergenic 40.0%
ACCATCAGTGAGTGTCAATT+GGG + chr5.3:20370398-20370417 None:intergenic 40.0%
ATAGTGGCTGGAAGTTTGAT+TGG - chr5.3:20371254-20371273 MS.gene069144:intron 40.0%
ATTGGTAGGGAGAAAGATAG+TGG - chr5.3:20371238-20371257 MS.gene069144:intron 40.0%
CATAAGGCGAAGAATGAATG+GGG - chr5.3:20370108-20370127 MS.gene069144:CDS 40.0%
CATCCTCTATTACCTCTGAT+TGG - chr5.3:20371021-20371040 MS.gene069144:CDS 40.0%
CATGGAAAGCTTGTAATGGT+TGG - chr5.3:20371057-20371076 MS.gene069144:intron 40.0%
CCTGCAAGTAAAGGAAAAAC+AGG + chr5.3:20371108-20371127 None:intergenic 40.0%
GACAAGCCTGGTATGAATAT+AGG - chr5.3:20370583-20370602 MS.gene069144:CDS 40.0%
GACTTGAGTAAACCAATCAG+AGG + chr5.3:20371036-20371055 None:intergenic 40.0%
GTCTCATGGAAAGCTTGTAA+TGG - chr5.3:20371053-20371072 MS.gene069144:intron 40.0%
GTGAAATTTGCCAAAGCCTT+TGG - chr5.3:20370640-20370659 MS.gene069144:CDS 40.0%
GTTGGCATAGTTACAGAAGT+GGG - chr5.3:20370265-20370284 MS.gene069144:intron 40.0%
TACGAACTATCCACTAGTTC+CGG - chr5.3:20370134-20370153 MS.gene069144:intron 40.0%
TCGAGTTCAAAATGTCGAAG+AGG + chr5.3:20370565-20370584 None:intergenic 40.0%
TGGCAAATTTCACAGCCATA+TGG + chr5.3:20370633-20370652 None:intergenic 40.0%
TGTTGGCATAGTTACAGAAG+TGG - chr5.3:20370264-20370283 MS.gene069144:intron 40.0%
TTCAAAGTTGGAGACAGAGT+AGG - chr5.3:20370304-20370323 MS.gene069144:intron 40.0%
! CCTTCTGGAGAAATTGAAAG+GGG + chr5.3:20369741-20369760 None:intergenic 40.0%
! GCTGGTTCCATCACTATATT+TGG + chr5.3:20370422-20370441 None:intergenic 40.0%
! GCTGTGTTGACGTAATCAAT+AGG + chr5.3:20371353-20371372 None:intergenic 40.0%
! TGGTGGCTTTTCTAACTCAA+TGG - chr5.3:20370447-20370466 MS.gene069144:CDS 40.0%
!! ATCCAAAGAAGGCATTTGGT+TGG - chr5.3:20369687-20369706 MS.gene069144:CDS 40.0%
!! CCTGTTTTTCCTTTACTTGC+AGG - chr5.3:20371105-20371124 MS.gene069144:intron 40.0%
!! TCCAAAGAAGGCATTTGGTT+GGG - chr5.3:20369688-20369707 MS.gene069144:CDS 40.0%
ACGAACTATCCACTAGTTCC+GGG - chr5.3:20370135-20370154 MS.gene069144:intron 45.0%
ATGTCACTCGGACCTTCATA+AGG - chr5.3:20370092-20370111 MS.gene069144:CDS 45.0%
CACATTAGCACCAAAGGCTT+TGG + chr5.3:20370653-20370672 None:intergenic 45.0%
CCATCAGTGAGTGTCAATTG+GGG + chr5.3:20370397-20370416 None:intergenic 45.0%
CCCCTTTCAATTTCTCCAGA+AGG - chr5.3:20369738-20369757 MS.gene069144:CDS 45.0%
GCATCAAGAGGTAACTCATC+TGG + chr5.3:20370502-20370521 None:intergenic 45.0%
GTAATACCAGCACAAAGGAG+AGG + chr5.3:20370532-20370551 None:intergenic 45.0%
GTCTTTCTCAGATGTTTCCC+TGG + chr5.3:20370043-20370062 None:intergenic 45.0%
TACGGTCACATTAGCACCAA+AGG + chr5.3:20370659-20370678 None:intergenic 45.0%
TCGTGATCCGAATCAGATGA+AGG - chr5.3:20370747-20370766 MS.gene069144:CDS 45.0%
TGATTACGTCAACACAGCGA+TGG - chr5.3:20371355-20371374 MS.gene069144:CDS 45.0%
TGGCTGGAAGTTTGATTGGT+GGG - chr5.3:20371258-20371277 MS.gene069144:intron 45.0%
! CATTTTGAACTCGACAAGCC+TGG - chr5.3:20370571-20370590 MS.gene069144:CDS 45.0%
! GGAAAAACAGGAAGCTCTAG+AGG + chr5.3:20371096-20371115 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.3:20370601-20370620 MS.gene069144:CDS 45.0%
!! GAGCATCCAAAGAAGGCATT+TGG - chr5.3:20369683-20369702 MS.gene069144:CDS 45.0%
AAAGCTTACCCCGGAACTAG+TGG + chr5.3:20370147-20370166 None:intergenic 50.0%
AGAAAAGCCACCATGAGTGC+TGG + chr5.3:20370440-20370459 None:intergenic 50.0%
CCCCAATTGACACTCACTGA+TGG - chr5.3:20370394-20370413 MS.gene069144:CDS 50.0%
CGAACTATCCACTAGTTCCG+GGG - chr5.3:20370136-20370155 MS.gene069144:intron 50.0%
GCCCAACCAAATGCCTTCTT+TGG + chr5.3:20369692-20369711 None:intergenic 50.0%
GCTGCAATGGGTACTTTGGA+TGG - chr5.3:20370973-20370992 MS.gene069144:CDS 50.0%
GTAGGCTGCAATGGGTACTT+TGG - chr5.3:20370969-20370988 MS.gene069144:CDS 50.0%
GTGGCTGGAAGTTTGATTGG+TGG - chr5.3:20371257-20371276 MS.gene069144:intron 50.0%
GTTGGAGACAGAGTAGGTGT+CGG - chr5.3:20370310-20370329 MS.gene069144:intron 50.0%
! AGTGATGGAACCAGCACTCA+TGG - chr5.3:20370427-20370446 MS.gene069144:CDS 50.0%
! CGGACGAGCACTTTGTGTTT+TGG - chr5.3:20370473-20370492 MS.gene069144:CDS 50.0%
! GTAGGGAGAAAGATAGTGGC+TGG - chr5.3:20371242-20371261 MS.gene069144:intron 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.3:20370607-20370626 MS.gene069144:CDS 50.0%
!! TCTTGGTGGACTTGGCCATA+TGG - chr5.3:20370615-20370634 MS.gene069144:CDS 50.0%
!!! GGAAGCTCTAGAGGCTTTTC+AGG + chr5.3:20371087-20371106 None:intergenic 50.0%
AGAGGAGCAGCAGCATCAAG+AGG + chr5.3:20370514-20370533 None:intergenic 55.0%
GATGGAACCAGCACTCATGG+TGG - chr5.3:20370430-20370449 MS.gene069144:CDS 55.0%
TGGGCAGCTAGAGACTCTTC+TGG - chr5.3:20369707-20369726 MS.gene069144:CDS 55.0%
!! CAGAGTAGGTGTCGGATGCT+TGG - chr5.3:20370318-20370337 MS.gene069144:intron 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.3:20370523-20370542 MS.gene069144:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20369662 20371451 20369662 ID=MS.gene069144
chr5.3 mRNA 20369662 20371451 20369662 ID=MS.gene069144.t1;Parent=MS.gene069144
chr5.3 exon 20371354 20371451 20371354 ID=MS.gene069144.t1.exon1;Parent=MS.gene069144.t1
chr5.3 CDS 20371354 20371451 20371354 ID=cds.MS.gene069144.t1;Parent=MS.gene069144.t1
chr5.3 exon 20370956 20371069 20370956 ID=MS.gene069144.t1.exon2;Parent=MS.gene069144.t1
chr5.3 CDS 20370956 20371069 20370956 ID=cds.MS.gene069144.t1;Parent=MS.gene069144.t1
chr5.3 exon 20370345 20370858 20370345 ID=MS.gene069144.t1.exon3;Parent=MS.gene069144.t1
chr5.3 CDS 20370345 20370858 20370345 ID=cds.MS.gene069144.t1;Parent=MS.gene069144.t1
chr5.3 exon 20369987 20370140 20369987 ID=MS.gene069144.t1.exon4;Parent=MS.gene069144.t1
chr5.3 CDS 20369987 20370140 20369987 ID=cds.MS.gene069144.t1;Parent=MS.gene069144.t1
chr5.3 exon 20369662 20369867 20369662 ID=MS.gene069144.t1.exon5;Parent=MS.gene069144.t1
chr5.3 CDS 20369662 20369867 20369662 ID=cds.MS.gene069144.t1;Parent=MS.gene069144.t1
Gene Sequence

>MS.gene069144

ATGGCAACACAACCTGAATTTGAGCATCCAAAGAAGGCATTTGGTTGGGCAGCTAGAGACTCTTCTGGTGTTCTCTCCCCTTTCAATTTCTCCAGAAGGTTCAATTCATTCATTATGATCACCTTTTCTTTCTAAACATAATATGCATGTAGTATTCATTATTTGTTGCACCATTTTATTTGCTCACTTTTTCTTTATAATGCTTTACATTTGTTTGTAAGGTGTTATTTCCTATTTCTACTAATTTTGACGAAAATATAAATAACATCATGAAAAAAGTGGAGGATATGCATTATCTTATTATGTCCTAGGTGTTTGATTATCATCATTATATAGAGATAACTAACCATATTCAATTTATATTACTATGTCAAATTTCCAGGGAAACATCTGAGAAAGACGTAGCATTAAAAGTGTTGTATTGTGGAATATGTCACTCGGACCTTCATAAGGCGAAGAATGAATGGGGCACTACGAACTATCCACTAGTTCCGGGGTAAGCTTTCTACTTTTATTCCATTTAAAAAAACCCAAACATATAAATCAAAATATAAATTCAATTTGATTCTAATTGGTGTAAGAAATATATACAGACATGAAATTGTTGGCATAGTTACAGAAGTGGGAAGCAAAGTGAGCAAGTTCAAAGTTGGAGACAGAGTAGGTGTCGGATGCTTGGTTGATTCTTGTCACTCATGTCAAAATTGTGTTGATAATCTTGAGAATTACTGTCCCCAATTGACACTCACTGATGGTTCCAAATATAGTGATGGAACCAGCACTCATGGTGGCTTTTCTAACTCAATGGTTTCGGACGAGCACTTTGTGTTTTGGATTCCAGATGAGTTACCTCTTGATGCTGCTGCTCCTCTCCTTTGTGCTGGTATTACTGTGTATAGTCCTCTTCGACATTTTGAACTCGACAAGCCTGGTATGAATATAGGTGTTGTTGGTCTTGGTGGACTTGGCCATATGGCTGTGAAATTTGCCAAAGCCTTTGGTGCTAATGTGACCGTAATTAGTACATCACCTAAAAAGGAAAAGGAAGCAATAGAACACTTAAGAGCTGACTCATTTTTAATAAGTCGTGATCCGAATCAGATGAAGGTACAATAATTAATTTAATTCACTATAAAAATTGTCATATAGTTGCTTATTTAGAGACGGAAAATGTCAAATCGATCGCTAAATTAAATTGATATTTAACATTAGTCATTAGTGACTAGTGGTCTCTAATCATTCACAAAATTACTCAACGGCCGATTTAGAAGTTGACAATTAGTGAACTAATCAATCTAGAATAATTTGTAGGCTGCAATGGGTACTTTGGATGGTATTATCGACACAGTTTCAGCGGATCATCCTCTATTACCTCTGATTGGTTTACTCAAGTCTCATGGAAAGCTTGTAATGGTTGGTGCACCTGAAAAGCCTCTAGAGCTTCCTGTTTTTCCTTTACTTGCAGGTATATATATAAGCATTTGAAAAATATATATACTTCCATATGATGCTAAATATGCCCTTCTAAATGTTTAAATTAAATTATGAACCTTTAAACTCCCAAGTATTTCACATTATTGGTAGGGAGAAAGATAGTGGCTGGAAGTTTGATTGGTGGGATCAAAGAGACTCAAGAAATGATTGATTTTGCAGCAGAACACAATGTAAAGCCTGATATTGAGATCATTCCTATTGATTACGTCAACACAGCGATGGAGCGCCTCCTTAAAGCAGATGTGAAATATCGATTTGTGATTGATATTGGAAGCACATTAAAATCAAGCTCTTGA
Protein sequence

>MS.gene069144.t1

MATQPEFEHPKKAFGWAARDSSGVLSPFNFSRRETSEKDVALKVLYCGICHSDLHKAKNEWGTTNYPLVPGHEIVGIVTEVGSKVSKFKVGDRVGVGCLVDSCHSCQNCVDNLENYCPQLTLTDGSKYSDGTSTHGGFSNSMVSDEHFVFWIPDELPLDAAAPLLCAGITVYSPLRHFELDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSPKKEKEAIEHLRADSFLISRDPNQMKAAMGTLDGIIDTVSADHPLLPLIGLLKSHGKLVMVGAPEKPLELPVFPLLAGRKIVAGSLIGGIKETQEMIDFAAEHNVKPDIEIIPIDYVNTAMERLLKADVKYRFVIDIGSTLKSSS