AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069151

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069151.t1 MTR_5g031250 96.122 361 14 0 1 361 1 361 0.0 725
MS.gene069151.t1 MTR_5g031230 81.994 361 65 0 1 361 1 361 0.0 610
MS.gene069151.t1 MTR_4g115300 81.717 361 66 0 1 361 1 361 0.0 607
MS.gene069151.t1 MTR_5g031390 80.055 361 72 0 1 361 1 361 0.0 592
MS.gene069151.t1 MTR_5g031310 80.055 361 71 1 1 360 1 361 0.0 590
MS.gene069151.t1 MTR_5g031210 79.501 361 74 0 1 361 1 361 0.0 585
MS.gene069151.t1 MTR_5g031190 78.393 361 78 0 1 361 1 361 0.0 582
MS.gene069151.t1 MTR_5g031305 79.501 361 73 1 1 360 1 361 0.0 581
MS.gene069151.t1 MTR_5g031300 79.006 362 75 1 1 361 1 362 0.0 580
MS.gene069151.t1 MTR_5g031180 80.609 361 70 0 1 361 1 361 0.0 568
MS.gene069151.t1 MTR_5g031360 76.923 351 81 0 10 360 12 362 0.0 561
MS.gene069151.t1 MTR_8g068190 78.056 360 79 0 1 360 1 360 0.0 560
MS.gene069151.t1 MTR_5g031250 96.266 241 9 0 1 241 1 241 4.28e-177 492
MS.gene069151.t1 MTR_8g092630 62.817 355 126 2 8 357 60 413 1.49e-152 436
MS.gene069151.t1 MTR_5g031430 62.029 345 131 0 14 358 14 358 5.22e-150 427
MS.gene069151.t1 MTR_1g025950 58.453 349 144 1 8 355 7 355 2.88e-138 397
MS.gene069151.t1 MTR_1g107425 53.353 343 160 0 11 353 9 351 7.31e-127 369
MS.gene069151.t1 MTR_2g009810 52.601 346 162 2 14 358 62 406 3.09e-119 351
MS.gene069151.t1 MTR_1g107425 54.770 283 127 1 11 293 9 290 5.39e-102 303
MS.gene069151.t1 MTR_2g009820 48.062 258 108 4 116 358 8 254 3.97e-65 207
MS.gene069151.t1 MTR_5g031290 78.070 114 25 0 247 360 80 193 4.39e-58 187
MS.gene069151.t1 MTR_5g031290 86.250 80 10 1 1 79 1 80 4.14e-44 151
MS.gene069151.t1 MTR_8g095220 25.714 280 178 7 25 278 68 343 8.44e-22 96.3
MS.gene069151.t1 MTR_8g095220 27.615 239 148 6 25 242 68 302 9.11e-22 94.7
MS.gene069151.t1 MTR_6g465010 26.380 326 212 12 15 325 3 315 3.07e-18 85.1
MS.gene069151.t1 MTR_6g466320 26.176 340 222 13 1 325 6 331 3.18e-18 85.1
MS.gene069151.t1 MTR_6g465010 26.176 340 222 13 1 325 6 331 3.18e-18 85.1
MS.gene069151.t1 MTR_0001s0660 25.166 302 202 10 38 318 35 333 5.92e-15 76.3
MS.gene069151.t1 MTR_3g089977 21.777 349 234 11 39 354 37 379 2.16e-14 73.9
MS.gene069151.t1 MTR_3g089940 22.071 367 245 12 11 344 12 370 3.83e-14 73.2
MS.gene069151.t1 MTR_3g089940 21.829 339 227 10 39 344 37 370 4.69e-14 73.2
MS.gene069151.t1 MTR_5g031380 66.667 39 13 0 80 118 19 57 6.91e-13 65.1
MS.gene069151.t1 MTR_3g089970 21.829 339 227 10 39 344 38 371 7.68e-13 69.3
MS.gene069151.t1 MTR_3g089940 21.493 335 230 9 11 319 12 339 4.86e-12 67.0
MS.gene069151.t1 MTR_3g089940 21.498 307 211 8 39 319 37 339 5.28e-12 66.6
MS.gene069151.t1 MTR_1g069430 24.854 342 216 13 1 312 1 331 1.43e-11 65.5
MS.gene069151.t1 MTR_5g045710 23.684 304 205 8 38 318 35 334 2.14e-11 65.1
MS.gene069151.t1 MTR_6g068930 25.065 387 240 18 2 353 4 375 5.98e-11 63.5
MS.gene069151.t1 MTR_7g099040 25.705 319 196 13 39 324 38 348 8.76e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069151.t1 AT4G37990 72.571 350 96 0 11 360 7 356 4.96e-175 491
MS.gene069151.t1 AT4G37970 65.070 355 124 0 6 360 7 361 4.34e-170 478
MS.gene069151.t1 AT4G37980 68.286 350 111 0 11 360 7 356 6.69e-166 468
MS.gene069151.t1 AT4G39330 61.281 359 139 0 1 359 1 359 1.71e-165 466
MS.gene069151.t1 AT2G21890 60.969 351 136 1 12 361 6 356 1.98e-157 447
MS.gene069151.t1 AT2G21730 61.080 352 135 2 12 361 6 357 2.62e-156 444
MS.gene069151.t1 AT4G39330 60.133 301 120 0 1 301 1 301 2.03e-132 381
MS.gene069151.t1 AT4G37970 62.976 289 107 0 6 294 7 295 6.46e-130 374
MS.gene069151.t1 AT4G37980 68.310 284 90 0 11 294 7 290 2.08e-128 370
MS.gene069151.t1 AT1G72680 50.146 343 170 1 16 358 14 355 5.10e-114 336
MS.gene069151.t1 AT3G19450 52.890 346 163 0 11 356 9 354 2.32e-113 334
MS.gene069151.t1 AT4G34230 53.890 347 160 0 7 353 4 350 1.70e-109 324
MS.gene069151.t1 AT4G34230 53.602 347 161 0 7 353 4 350 1.50e-108 322
MS.gene069151.t1 AT4G37970 62.762 239 89 0 6 244 7 245 9.35e-105 308
MS.gene069151.t1 AT4G37970 63.291 237 87 0 6 242 7 243 2.14e-104 308
MS.gene069151.t1 AT5G63620 25.979 281 176 8 25 278 70 345 1.34e-22 98.6
MS.gene069151.t1 AT5G63620 25.979 281 176 8 25 278 70 345 1.49e-22 98.6
MS.gene069151.t1 AT5G63620 26.778 239 150 6 25 242 70 304 1.64e-22 97.1
MS.gene069151.t1 AT5G51970 25.490 306 202 9 35 325 39 333 1.13e-17 83.6
MS.gene069151.t1 AT5G51970 25.490 306 202 9 35 325 39 333 1.13e-17 83.6
MS.gene069151.t1 AT5G43940 25.215 349 218 12 39 352 36 376 1.84e-15 77.4
MS.gene069151.t1 AT5G43940 25.215 349 218 12 39 352 48 388 2.20e-15 77.0
MS.gene069151.t1 AT1G22430 25.078 319 201 12 39 324 42 355 7.02e-15 75.5
MS.gene069151.t1 AT1G22430 25.078 319 201 12 39 324 42 355 7.02e-15 75.5
MS.gene069151.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.09e-13 71.6
MS.gene069151.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.29e-13 70.9
MS.gene069151.t1 AT1G22430 26.000 200 129 5 39 221 42 239 1.29e-13 70.9
MS.gene069151.t1 AT1G32780 26.099 364 218 15 39 358 37 393 1.30e-12 68.9
MS.gene069151.t1 AT1G77120 20.672 387 265 11 1 354 1 378 2.64e-11 64.7

Find 88 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGTCCTCTTCGATATTT+TGG 0.153434 5.3:+20281922 MS.gene069151:CDS
CCCTCACATTCCTTTAATAA+TGG 0.196862 5.3:+20282350 MS.gene069151:CDS
TATCGATTTGTGATTGATAT+TGG 0.198958 5.3:+20282693 MS.gene069151:CDS
TTCTTTGGATGTTCAAATTC+AGG 0.206123 5.3:-20281175 None:intergenic
TATTGCTTCTTTCTCTTTGT+TGG 0.211892 5.3:-20282058 None:intergenic
CTATATGTTTGTGTTTGTTT+AGG 0.221743 5.3:-20281760 None:intergenic
AACAACTTACCCATTATTAA+AGG 0.228051 5.3:-20282360 None:intergenic
GCCCAACCAAATGCCTTCTT+TGG 0.260645 5.3:-20281190 None:intergenic
TATATGTTTGTGTTTGTTTA+GGG 0.265623 5.3:-20281759 None:intergenic
CCTCACATTCCTTTAATAAT+GGG 0.310674 5.3:+20282351 MS.gene069151:CDS
CCCCTTTCAACTTCTCTAGA+AGG 0.321261 5.3:+20281239 MS.gene069151:CDS
TCTTGGTGGACTTGGTCACT+TGG 0.333930 5.3:+20281981 MS.gene069151:CDS
ACGATATCTGGCAGTGGAAT+TGG 0.337964 5.3:+20282543 MS.gene069151:CDS
ATTGCTTCTTTCTCTTTGTT+GGG 0.338643 5.3:-20282057 None:intergenic
GTGAAGTTTGCAAAAGCTTT+TGG 0.362966 5.3:+20282006 MS.gene069151:CDS
GAACATCCAAAGAAGGCATT+TGG 0.363606 5.3:+20281184 MS.gene069151:CDS
TCTACCTATCCACTAGTTCC+TGG 0.381163 5.3:+20281499 MS.gene069151:CDS
GCAGGGAGAAAGACGATATC+TGG 0.389267 5.3:+20282531 MS.gene069151:intron
GATATTTCACATCTGCTTTG+AGG 0.391986 5.3:-20282674 None:intergenic
CATTTAAAGTACAGTATTGC+GGG 0.393811 5.3:+20281431 MS.gene069151:CDS
GGGAGCTCCGGAGGCTTCTC+AGG 0.395546 5.3:-20282330 None:intergenic
ATAGGTATTGTTGGTCTTGG+TGG 0.404910 5.3:+20281967 MS.gene069151:CDS
ATTGTTGGTCTTGGTGGACT+TGG 0.407291 5.3:+20281973 MS.gene069151:CDS
CATCCTCTCTTACCTCTAGT+TGG 0.407705 5.3:+20282267 MS.gene069151:CDS
TTGTACAAAATTTAAGAACT+AGG 0.429877 5.3:-20282729 None:intergenic
ATATCACGGAAAACTTATAA+TGG 0.431863 5.3:+20282299 MS.gene069151:CDS
GATTACAGGCATGAGGTTGC+TGG 0.448995 5.3:+20281613 MS.gene069151:intron
TCCAAAGAAGGCATTTGGTT+GGG 0.449991 5.3:+20281189 MS.gene069151:CDS
GTCGTGAACTTTCAGAGAAA+CGG 0.456414 5.3:+20281393 MS.gene069151:intron
AATATAGGTATTGTTGGTCT+TGG 0.457645 5.3:+20281964 MS.gene069151:CDS
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.3:-20281865 None:intergenic
TCGTGAACTTTCAGAGAAAC+GGG 0.468716 5.3:+20281394 MS.gene069151:intron
GGTATGAATATAGGTATTGT+TGG 0.472768 5.3:+20281958 MS.gene069151:CDS
ATAGGCTGCGATGGGTACTT+TGG 0.475496 5.3:+20282215 MS.gene069151:intron
TGCTAAACACGATATAAAAC+CGG 0.477369 5.3:+20282602 MS.gene069151:CDS
ATAGGTATGACTTCGATGTC+CGG 0.491179 5.3:-20282621 None:intergenic
GACAAGCCTGGTATGAATAT+AGG 0.491634 5.3:+20281949 MS.gene069151:CDS
AGCGATGGAACCATCACATA+TGG 0.493438 5.3:+20281793 MS.gene069151:CDS
TAAGAAATGAATCAGCTCCT+AGG 0.495743 5.3:-20282086 None:intergenic
ACCTTCTAGAGAAGTTGAAA+GGG 0.496182 5.3:-20281240 None:intergenic
CTTGGTGGACTTGGTCACTT+GGG 0.501069 5.3:+20281982 MS.gene069151:CDS
GCTGCTCCTCTCCTTTGTGC+TGG 0.501431 5.3:+20281889 MS.gene069151:CDS
TGGGCAGCAAGAGACACTTC+TGG 0.507758 5.3:+20281208 MS.gene069151:CDS
AAAGGAATGTGAGGGAGCTC+CGG 0.512634 5.3:-20282342 None:intergenic
AACCTTCTAGAGAAGTTGAA+AGG 0.514607 5.3:-20281241 None:intergenic
AGTTGGAGACAAAGTGGGTG+TGG 0.517700 5.3:+20281675 MS.gene069151:CDS
TTTATAGTTCGCATTCCTGA+TGG 0.525602 5.3:+20281850 MS.gene069151:CDS
CGGTGCACCTGAGAAGCCTC+CGG 0.529283 5.3:+20282323 MS.gene069151:CDS
ATCCAAAGAAGGCATTTGGT+TGG 0.531418 5.3:+20281188 MS.gene069151:CDS
TGAATTTGAACATCCAAAGA+AGG 0.532781 5.3:+20281177 MS.gene069151:CDS
TGGAGGGTACTCTGACTCAA+TGG 0.539173 5.3:+20281813 MS.gene069151:CDS
CCCATTATTAAAGGAATGTG+AGG 0.542582 5.3:-20282351 None:intergenic
GTTGGATTATTAAAATATCA+CGG 0.550914 5.3:+20282285 MS.gene069151:CDS
AAAGTCGAGAAGTTCAAAGT+TGG 0.554625 5.3:+20281658 MS.gene069151:CDS
GGACATCGAAGTCATACCTA+TGG 0.559852 5.3:+20282623 MS.gene069151:CDS
ATATCTGGCAGTGGAATTGG+AGG 0.561378 5.3:+20282546 MS.gene069151:CDS
CACGGAAAACTTATAATGGT+CGG 0.562740 5.3:+20282303 MS.gene069151:CDS
CTACCTATCCACTAGTTCCT+GGG 0.565757 5.3:+20281500 MS.gene069151:CDS
GCTGCGATGGGTACTTTGGA+TGG 0.570444 5.3:+20282219 MS.gene069151:CDS
CATACGTACCCAGGAACTAG+TGG 0.581134 5.3:-20281508 None:intergenic
GCATTTAAAGTACAGTATTG+CGG 0.588287 5.3:+20281430 MS.gene069151:CDS
GATATATGATTACAGGCATG+AGG 0.600442 5.3:+20281606 MS.gene069151:intron
GCTGGTATAGTCACAGAAGT+GGG 0.600918 5.3:+20281631 MS.gene069151:CDS
AAAGAAGCAATAGAAAACCT+AGG 0.602446 5.3:+20282069 MS.gene069151:CDS
TGCTGGTATAGTCACAGAAG+TGG 0.607495 5.3:+20281630 MS.gene069151:CDS
TCATAACCAAGATAAAATGC+AGG 0.607689 5.3:+20282113 MS.gene069151:CDS
GCTGTGTTAACGTAATCCAT+AGG 0.613883 5.3:-20282639 None:intergenic
GTTCAAAGTTGGAGACAAAG+TGG 0.616401 5.3:+20281669 MS.gene069151:CDS
AGAGTACCCTCCATATGTGA+TGG 0.616852 5.3:-20281803 None:intergenic
AGAAAGACGATATCTGGCAG+TGG 0.618866 5.3:+20282537 MS.gene069151:CDS
GATGGAACCATCACATATGG+AGG 0.621420 5.3:+20281796 MS.gene069151:CDS
GGAATGTGAGGGAGCTCCGG+AGG 0.622096 5.3:-20282339 None:intergenic
TATCATTGATACAGTTTCAG+CGG 0.623020 5.3:+20282242 MS.gene069151:CDS
GTAATACCAGCACAAAGGAG+AGG 0.623289 5.3:-20281895 None:intergenic
AATCCAACTAGAGGTAAGAG+AGG 0.623627 5.3:-20282270 None:intergenic
GTACCCAGGAACTAGTGGAT+AGG 0.637899 5.3:-20281503 None:intergenic
CCATTATTAAAGGAATGTGA+GGG 0.639713 5.3:-20282350 None:intergenic
TTCAAAGTTGGAGACAAAGT+GGG 0.640553 5.3:+20281670 MS.gene069151:CDS
GTTGGAGACAAAGTGGGTGT+GGG 0.640763 5.3:+20281676 MS.gene069151:CDS
AGAGGAGCAGCAGCTTCAAG+TGG 0.642094 5.3:-20281877 None:intergenic
TCGAGTCCAAAATATCGAAG+AGG 0.642096 5.3:-20281928 None:intergenic
CCTTCTAGAGAAGTTGAAAG+GGG 0.645104 5.3:-20281239 None:intergenic
TATAGTGCGAAATACAGCGA+TGG 0.656053 5.3:+20281778 MS.gene069151:CDS
TCGCGCATGCCAAAATTGTG+AGG 0.663146 5.3:+20281717 MS.gene069151:CDS
ACAATACCTATATTCATACC+AGG 0.667309 5.3:-20281955 None:intergenic
ATGGAACCATCACATATGGA+GGG 0.675053 5.3:+20281797 MS.gene069151:CDS
GTATTGCGGGATATGTCACT+CGG 0.700120 5.3:+20281444 MS.gene069151:CDS
ACACAGTAATACCAGCACAA+AGG 0.710961 5.3:-20281900 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATAATAATAATCTTAAT+TGG + chr5.3:20282154-20282173 MS.gene069151:intron 10.0%
!! TAAGATTATTATTATAACTT+TGG - chr5.3:20282152-20282171 None:intergenic 10.0%
!!! ATTTTGATGATATTATTTAT+TGG + chr5.3:20282509-20282528 MS.gene069151:intron 10.0%
!!! TTTTATACATTTATTTTCAT+AGG - chr5.3:20282489-20282508 None:intergenic 10.0%
!! AATTAAAAATTCCTAAGATA+TGG - chr5.3:20281554-20281573 None:intergenic 15.0%
!!! ATTAATGTCAATGATTTTAT+AGG + chr5.3:20282197-20282216 MS.gene069151:intron 15.0%
!!! TTATTTTCATAGGTATAAAA+GGG - chr5.3:20282479-20282498 None:intergenic 15.0%
!!! TTGACAAAATAATTATTTCT+TGG + chr5.3:20281367-20281386 MS.gene069151:intron 15.0%
!!! TTTATTTTCATAGGTATAAA+AGG - chr5.3:20282480-20282499 None:intergenic 15.0%
!! AAAAGATGTATAAAATGAAG+AGG - chr5.3:20281281-20281300 None:intergenic 20.0%
!! GTTGGATTATTAAAATATCA+CGG + chr5.3:20282285-20282304 MS.gene069151:CDS 20.0%
!! TATATGTTTGTGTTTGTTTA+GGG - chr5.3:20281762-20281781 None:intergenic 20.0%
!! TCATATGATCAAAAATGAAT+GGG + chr5.3:20281471-20281490 MS.gene069151:CDS 20.0%
!! TCTAATTGATATATGATTAC+AGG + chr5.3:20281599-20281618 MS.gene069151:intron 20.0%
!! TTCATATGATCAAAAATGAA+TGG + chr5.3:20281470-20281489 MS.gene069151:CDS 20.0%
!!! TATTTTAATAATCCAACTAG+AGG - chr5.3:20282282-20282301 None:intergenic 20.0%
! AACAACTTACCCATTATTAA+AGG - chr5.3:20282363-20282382 None:intergenic 25.0%
! ACTTTAACAAACCATATCTT+AGG + chr5.3:20281540-20281559 MS.gene069151:intron 25.0%
! ATATCACGGAAAACTTATAA+TGG + chr5.3:20282299-20282318 MS.gene069151:CDS 25.0%
! CAATTAGAATAAAATTGCAG+TGG - chr5.3:20281587-20281606 None:intergenic 25.0%
! CATATGATCAAAAATGAATG+GGG + chr5.3:20281472-20281491 MS.gene069151:CDS 25.0%
! CTATATGTTTGTGTTTGTTT+AGG - chr5.3:20281763-20281782 None:intergenic 25.0%
! GATGATATTATTTATTGGCA+GGG + chr5.3:20282514-20282533 MS.gene069151:intron 25.0%
! TGATGATATTATTTATTGGC+AGG + chr5.3:20282513-20282532 MS.gene069151:intron 25.0%
!! TTTATACCTGCATTTTATCT+TGG - chr5.3:20282122-20282141 None:intergenic 25.0%
!!! TATCGATTTGTGATTGATAT+TGG + chr5.3:20282693-20282712 MS.gene069151:CDS 25.0%
AAAGAAGCAATAGAAAACCT+AGG + chr5.3:20282069-20282088 MS.gene069151:CDS 30.0%
ACAATACCTATATTCATACC+AGG - chr5.3:20281958-20281977 None:intergenic 30.0%
AGATGTATAAAATGAAGAGG+TGG - chr5.3:20281278-20281297 None:intergenic 30.0%
ATTGCTTCTTTCTCTTTGTT+GGG - chr5.3:20282060-20282079 None:intergenic 30.0%
CCATTATTAAAGGAATGTGA+GGG - chr5.3:20282353-20282372 None:intergenic 30.0%
CCTCACATTCCTTTAATAAT+GGG + chr5.3:20282351-20282370 MS.gene069151:CDS 30.0%
TATCATTGATACAGTTTCAG+CGG + chr5.3:20282242-20282261 MS.gene069151:CDS 30.0%
TATTGCTTCTTTCTCTTTGT+TGG - chr5.3:20282061-20282080 None:intergenic 30.0%
TCATAACCAAGATAAAATGC+AGG + chr5.3:20282113-20282132 MS.gene069151:CDS 30.0%
TGAATTTGAACATCCAAAGA+AGG + chr5.3:20281177-20281196 MS.gene069151:CDS 30.0%
TGCTAAACACGATATAAAAC+CGG + chr5.3:20282602-20282621 MS.gene069151:CDS 30.0%
TTCTTTGGATGTTCAAATTC+AGG - chr5.3:20281178-20281197 None:intergenic 30.0%
! AATATAGGTATTGTTGGTCT+TGG + chr5.3:20281964-20281983 MS.gene069151:CDS 30.0%
! CAAGATTTTCCTCACAATTT+TGG - chr5.3:20281729-20281748 None:intergenic 30.0%
! CATTTAAAGTACAGTATTGC+GGG + chr5.3:20281431-20281450 MS.gene069151:CDS 30.0%
! GCATTTAAAGTACAGTATTG+CGG + chr5.3:20281430-20281449 MS.gene069151:CDS 30.0%
! GGTATGAATATAGGTATTGT+TGG + chr5.3:20281958-20281977 MS.gene069151:CDS 30.0%
! TATAGTCCTCTTCGATATTT+TGG + chr5.3:20281922-20281941 MS.gene069151:CDS 30.0%
AAAGTCGAGAAGTTCAAAGT+TGG + chr5.3:20281658-20281677 MS.gene069151:CDS 35.0%
AAATTCAAACACTATAGCCG+CGG - chr5.3:20282399-20282418 None:intergenic 35.0%
CACGGAAAACTTATAATGGT+CGG + chr5.3:20282303-20282322 MS.gene069151:CDS 35.0%
CCCATTATTAAAGGAATGTG+AGG - chr5.3:20282354-20282373 None:intergenic 35.0%
CCCTCACATTCCTTTAATAA+TGG + chr5.3:20282350-20282369 MS.gene069151:CDS 35.0%
GATATATGATTACAGGCATG+AGG + chr5.3:20281606-20281625 MS.gene069151:intron 35.0%
GATATTTCACATCTGCTTTG+AGG - chr5.3:20282677-20282696 None:intergenic 35.0%
GATTGTGATCGATATGTTCA+CGG + chr5.3:20282447-20282466 MS.gene069151:intron 35.0%
GTATAAAATGAAGAGGTGGA+TGG - chr5.3:20281274-20281293 None:intergenic 35.0%
TAAGAAATGAATCAGCTCCT+AGG - chr5.3:20282089-20282108 None:intergenic 35.0%
TTCAAAGTTGGAGACAAAGT+GGG + chr5.3:20281670-20281689 MS.gene069151:CDS 35.0%
TTTATAGTTCGCATTCCTGA+TGG + chr5.3:20281850-20281869 MS.gene069151:CDS 35.0%
! AACCTTCTAGAGAAGTTGAA+AGG - chr5.3:20281244-20281263 None:intergenic 35.0%
! AATGATTTTATAGGCTGCGA+TGG + chr5.3:20282206-20282225 MS.gene069151:intron 35.0%
! ACCTTCTAGAGAAGTTGAAA+GGG - chr5.3:20281243-20281262 None:intergenic 35.0%
! ATGATTTTATAGGCTGCGAT+GGG + chr5.3:20282207-20282226 MS.gene069151:intron 35.0%
! GTGAAGTTTGCAAAAGCTTT+TGG + chr5.3:20282006-20282025 MS.gene069151:CDS 35.0%
AATCCAACTAGAGGTAAGAG+AGG - chr5.3:20282273-20282292 None:intergenic 40.0%
AATTCAAACACTATAGCCGC+GGG - chr5.3:20282398-20282417 None:intergenic 40.0%
ACACAGTAATACCAGCACAA+AGG - chr5.3:20281903-20281922 None:intergenic 40.0%
AGAAACGGGTGAAAAAGATG+TGG + chr5.3:20281408-20281427 MS.gene069151:CDS 40.0%
ATAGGTATGACTTCGATGTC+CGG - chr5.3:20282624-20282643 None:intergenic 40.0%
ATGGAACCATCACATATGGA+GGG + chr5.3:20281797-20281816 MS.gene069151:CDS 40.0%
GACAAGCCTGGTATGAATAT+AGG + chr5.3:20281949-20281968 MS.gene069151:CDS 40.0%
GCTGTGTTAACGTAATCCAT+AGG - chr5.3:20282642-20282661 None:intergenic 40.0%
GTCGTGAACTTTCAGAGAAA+CGG + chr5.3:20281393-20281412 MS.gene069151:intron 40.0%
GTTCAAAGTTGGAGACAAAG+TGG + chr5.3:20281669-20281688 MS.gene069151:CDS 40.0%
TAAAATGAAGAGGTGGATGG+AGG - chr5.3:20281271-20281290 None:intergenic 40.0%
TATAGTGCGAAATACAGCGA+TGG + chr5.3:20281778-20281797 MS.gene069151:CDS 40.0%
TCGAGTCCAAAATATCGAAG+AGG - chr5.3:20281931-20281950 None:intergenic 40.0%
TCGTGAACTTTCAGAGAAAC+GGG + chr5.3:20281394-20281413 MS.gene069151:intron 40.0%
TGTTGTGAAAAATGTTCCCG+CGG + chr5.3:20282379-20282398 MS.gene069151:intron 40.0%
! CCTTCTAGAGAAGTTGAAAG+GGG - chr5.3:20281242-20281261 None:intergenic 40.0%
!! ATAGGTATTGTTGGTCTTGG+TGG + chr5.3:20281967-20281986 MS.gene069151:CDS 40.0%
!! ATCCAAAGAAGGCATTTGGT+TGG + chr5.3:20281188-20281207 MS.gene069151:CDS 40.0%
!! GAACATCCAAAGAAGGCATT+TGG + chr5.3:20281184-20281203 MS.gene069151:CDS 40.0%
!! TCCAAAGAAGGCATTTGGTT+GGG + chr5.3:20281189-20281208 MS.gene069151:CDS 40.0%
ACGATATCTGGCAGTGGAAT+TGG + chr5.3:20282543-20282562 MS.gene069151:CDS 45.0%
AGAAAGACGATATCTGGCAG+TGG + chr5.3:20282537-20282556 MS.gene069151:CDS 45.0%
AGCGATGGAACCATCACATA+TGG + chr5.3:20281793-20281812 MS.gene069151:CDS 45.0%
ATATCTGGCAGTGGAATTGG+AGG + chr5.3:20282546-20282565 MS.gene069151:CDS 45.0%
CATCCTCTCTTACCTCTAGT+TGG + chr5.3:20282267-20282286 MS.gene069151:CDS 45.0%
CCCCTTTCAACTTCTCTAGA+AGG + chr5.3:20281239-20281258 MS.gene069151:CDS 45.0%
CTACCTATCCACTAGTTCCT+GGG + chr5.3:20281500-20281519 MS.gene069151:CDS 45.0%
GATGGAACCATCACATATGG+AGG + chr5.3:20281796-20281815 MS.gene069151:CDS 45.0%
GCTGGTATAGTCACAGAAGT+GGG + chr5.3:20281631-20281650 MS.gene069151:CDS 45.0%
GGACATCGAAGTCATACCTA+TGG + chr5.3:20282623-20282642 MS.gene069151:CDS 45.0%
GTAATACCAGCACAAAGGAG+AGG - chr5.3:20281898-20281917 None:intergenic 45.0%
GTATTGCGGGATATGTCACT+CGG + chr5.3:20281444-20281463 MS.gene069151:CDS 45.0%
TCTACCTATCCACTAGTTCC+TGG + chr5.3:20281499-20281518 MS.gene069151:CDS 45.0%
TGCTGGTATAGTCACAGAAG+TGG + chr5.3:20281630-20281649 MS.gene069151:CDS 45.0%
! AGAGTACCCTCCATATGTGA+TGG - chr5.3:20281806-20281825 None:intergenic 45.0%
!! ATTGTTGGTCTTGGTGGACT+TGG + chr5.3:20281973-20281992 MS.gene069151:CDS 45.0%
!! TATTTTGGACTCGACAAGCC+TGG + chr5.3:20281937-20281956 MS.gene069151:CDS 45.0%
AAAGGAATGTGAGGGAGCTC+CGG - chr5.3:20282345-20282364 None:intergenic 50.0%
AGTTCGAAGCATACGTACCC+AGG - chr5.3:20281520-20281539 None:intergenic 50.0%
AGTTGGAGACAAAGTGGGTG+TGG + chr5.3:20281675-20281694 MS.gene069151:CDS 50.0%
ATAGGCTGCGATGGGTACTT+TGG + chr5.3:20282215-20282234 MS.gene069151:intron 50.0%
CATACGTACCCAGGAACTAG+TGG - chr5.3:20281511-20281530 None:intergenic 50.0%
GATTACAGGCATGAGGTTGC+TGG + chr5.3:20281613-20281632 MS.gene069151:intron 50.0%
GCAGGGAGAAAGACGATATC+TGG + chr5.3:20282531-20282550 MS.gene069151:intron 50.0%
GCCCAACCAAATGCCTTCTT+TGG - chr5.3:20281193-20281212 None:intergenic 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.3:20281868-20281887 None:intergenic 50.0%
GTTGGAGACAAAGTGGGTGT+GGG + chr5.3:20281676-20281695 MS.gene069151:CDS 50.0%
TCGCGCATGCCAAAATTGTG+AGG + chr5.3:20281717-20281736 MS.gene069151:CDS 50.0%
! GTACCCAGGAACTAGTGGAT+AGG - chr5.3:20281506-20281525 None:intergenic 50.0%
!! CTTGGTGGACTTGGTCACTT+GGG + chr5.3:20281982-20282001 MS.gene069151:CDS 50.0%
!! TCTTGGTGGACTTGGTCACT+TGG + chr5.3:20281981-20282000 MS.gene069151:CDS 50.0%
!! TGGAGGGTACTCTGACTCAA+TGG + chr5.3:20281813-20281832 MS.gene069151:CDS 50.0%
AGAGGAGCAGCAGCTTCAAG+TGG - chr5.3:20281880-20281899 None:intergenic 55.0%
GCTGCGATGGGTACTTTGGA+TGG + chr5.3:20282219-20282238 MS.gene069151:CDS 55.0%
TGGGCAGCAAGAGACACTTC+TGG + chr5.3:20281208-20281227 MS.gene069151:CDS 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG + chr5.3:20281889-20281908 MS.gene069151:CDS 60.0%
CGGTGCACCTGAGAAGCCTC+CGG + chr5.3:20282323-20282342 MS.gene069151:CDS 65.0%
GGAATGTGAGGGAGCTCCGG+AGG - chr5.3:20282342-20282361 None:intergenic 65.0%
GGGAGCTCCGGAGGCTTCTC+AGG - chr5.3:20282333-20282352 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20281163 20282740 20281163 ID=MS.gene069151
chr5.3 mRNA 20281163 20282740 20281163 ID=MS.gene069151.t1;Parent=MS.gene069151
chr5.3 exon 20281163 20281260 20281163 ID=MS.gene069151.t1.exon1;Parent=MS.gene069151.t1
chr5.3 CDS 20281163 20281260 20281163 ID=cds.MS.gene069151.t1;Parent=MS.gene069151.t1
chr5.3 exon 20281408 20281521 20281408 ID=MS.gene069151.t1.exon2;Parent=MS.gene069151.t1
chr5.3 CDS 20281408 20281521 20281408 ID=cds.MS.gene069151.t1;Parent=MS.gene069151.t1
chr5.3 exon 20281621 20282134 20281621 ID=MS.gene069151.t1.exon3;Parent=MS.gene069151.t1
chr5.3 CDS 20281621 20282134 20281621 ID=cds.MS.gene069151.t1;Parent=MS.gene069151.t1
chr5.3 exon 20282219 20282372 20282219 ID=MS.gene069151.t1.exon4;Parent=MS.gene069151.t1
chr5.3 CDS 20282219 20282372 20282219 ID=cds.MS.gene069151.t1;Parent=MS.gene069151.t1
chr5.3 exon 20282535 20282740 20282535 ID=MS.gene069151.t1.exon5;Parent=MS.gene069151.t1
chr5.3 CDS 20282535 20282740 20282535 ID=cds.MS.gene069151.t1;Parent=MS.gene069151.t1
Gene Sequence

>MS.gene069151

ATGGCAACACAACCTGAATTTGAACATCCAAAGAAGGCATTTGGTTGGGCAGCAAGAGACACTTCTGGTGTTCTCTCCCCTTTCAACTTCTCTAGAAGGTTCATTCCTCCATCCACCTCTTCATTTTATACATCTTTTTAACTAACAAAGTGCTAACAATTGTTGTAAAGACAGAAATATTTTAATATGTTGAGAACAATGTTGTTGACAAAATAATTATTTCTTGGTGTGTCGTGAACTTTCAGAGAAACGGGTGAAAAAGATGTGGCATTTAAAGTACAGTATTGCGGGATATGTCACTCGGATCTTCATATGATCAAAAATGAATGGGGCATGTCTACCTATCCACTAGTTCCTGGGTACGTATGCTTCGAACTACTTTAACAAACCATATCTTAGGAATTTTTAATTTTACTATTTTCCACTGCAATTTTATTCTAATTGATATATGATTACAGGCATGAGGTTGCTGGTATAGTCACAGAAGTGGGAAGCAAAGTCGAGAAGTTCAAAGTTGGAGACAAAGTGGGTGTGGGATGCTTAGTTGATTCATGTCGCGCATGCCAAAATTGTGAGGAAAATCTTGAGAATTATTGCCCTAAACAAACACAAACATATAGTGCGAAATACAGCGATGGAACCATCACATATGGAGGGTACTCTGACTCAATGGTTGCAGATGAGCACTTTATAGTTCGCATTCCTGATGGCTTACCACTTGAAGCTGCTGCTCCTCTCCTTTGTGCTGGTATTACTGTGTATAGTCCTCTTCGATATTTTGGACTCGACAAGCCTGGTATGAATATAGGTATTGTTGGTCTTGGTGGACTTGGTCACTTGGGTGTGAAGTTTGCAAAAGCTTTTGGTGCTAATGTAACAGTAATTAGTACATCACCCAACAAAGAGAAAGAAGCAATAGAAAACCTAGGAGCTGATTCATTTCTTATAAGTCATAACCAAGATAAAATGCAGGTATAAATACAAAACCAAAGTTATAATAATAATCTTAATTGGAAATTGTAATTGATTAACTTATTAATGTCAATGATTTTATAGGCTGCGATGGGTACTTTGGATGGTATCATTGATACAGTTTCAGCGGATCATCCTCTCTTACCTCTAGTTGGATTATTAAAATATCACGGAAAACTTATAATGGTCGGTGCACCTGAGAAGCCTCCGGAGCTCCCTCACATTCCTTTAATAATGGGTAAGTTGTTGTGAAAAATGTTCCCGCGGCTATAGTGTTTGAATTTTATCATAACTATCTCTAAATTTATGATTGATTGTGATCGATATGTTCACGGTTTAAACCCTTTTATACCTATGAAAATAAATGTATAAAAATTTTGATGATATTATTTATTGGCAGGGAGAAAGACGATATCTGGCAGTGGAATTGGAGGCATGAAAGAGACACAAGAAATGATTGATTTTGCTGCTAAACACGATATAAAACCGGACATCGAAGTCATACCTATGGATTACGTTAACACAGCAATGAAGCGACTCCTCAAAGCAGATGTGAAATATCGATTTGTGATTGATATTGGAAACACTTTAAAACCTAGTTCTTAA
Protein sequence

>MS.gene069151.t1

MATQPEFEHPKKAFGWAARDTSGVLSPFNFSRRETGEKDVAFKVQYCGICHSDLHMIKNEWGMSTYPLVPGHEVAGIVTEVGSKVEKFKVGDKVGVGCLVDSCRACQNCEENLENYCPKQTQTYSAKYSDGTITYGGYSDSMVADEHFIVRIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGMNIGIVGLGGLGHLGVKFAKAFGANVTVISTSPNKEKEAIENLGADSFLISHNQDKMQAAMGTLDGIIDTVSADHPLLPLVGLLKYHGKLIMVGAPEKPPELPHIPLIMGRKTISGSGIGGMKETQEMIDFAAKHDIKPDIEVIPMDYVNTAMKRLLKADVKYRFVIDIGNTLKPSS