AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070877

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070877.t1 MTR_5g031310 96.676 361 12 0 1 361 1 361 0.0 721
MS.gene070877.t1 MTR_5g031305 94.183 361 21 0 1 361 1 361 0.0 689
MS.gene070877.t1 MTR_5g031300 84.488 361 56 0 1 361 1 361 0.0 630
MS.gene070877.t1 MTR_5g031360 86.835 357 47 0 5 361 6 362 0.0 627
MS.gene070877.t1 MTR_5g031230 82.271 361 63 1 1 361 1 360 0.0 599
MS.gene070877.t1 MTR_4g115300 81.921 354 64 0 8 361 7 360 0.0 597
MS.gene070877.t1 MTR_5g031390 82.486 354 62 0 8 361 7 360 0.0 593
MS.gene070877.t1 MTR_5g031250 79.501 361 73 1 1 361 1 360 0.0 578
MS.gene070877.t1 MTR_5g031210 78.814 354 75 0 8 361 7 360 0.0 561
MS.gene070877.t1 MTR_5g031190 75.921 353 85 0 9 361 8 360 0.0 555
MS.gene070877.t1 MTR_5g031180 79.379 354 73 0 8 361 7 360 0.0 549
MS.gene070877.t1 MTR_8g068190 77.966 354 78 0 8 361 7 360 0.0 537
MS.gene070877.t1 MTR_8g092630 60.497 362 137 2 2 358 53 413 7.78e-151 431
MS.gene070877.t1 MTR_5g031430 58.841 345 142 0 15 359 14 358 3.89e-143 410
MS.gene070877.t1 MTR_5g031250 80.992 242 45 1 1 242 1 241 1.75e-139 397
MS.gene070877.t1 MTR_1g025950 56.609 348 150 1 10 356 8 355 6.42e-133 384
MS.gene070877.t1 MTR_1g107425 51.603 343 166 0 12 354 9 351 3.17e-122 357
MS.gene070877.t1 MTR_2g009810 53.314 347 158 3 15 359 62 406 8.67e-117 345
MS.gene070877.t1 MTR_1g107425 51.211 289 140 1 12 299 9 297 3.69e-96 288
MS.gene070877.t1 MTR_2g009820 51.293 232 110 2 131 359 23 254 4.44e-63 202
MS.gene070877.t1 MTR_5g031290 75.439 114 28 0 248 361 80 193 1.30e-53 176
MS.gene070877.t1 MTR_5g031290 81.481 81 13 2 1 80 1 80 2.10e-40 141
MS.gene070877.t1 MTR_0001s0660 28.146 302 189 10 39 317 35 331 4.20e-21 94.7
MS.gene070877.t1 MTR_8g095220 25.424 295 192 6 11 279 51 343 5.57e-21 94.0
MS.gene070877.t1 MTR_8g095220 26.772 254 163 5 11 243 51 302 6.16e-20 89.7
MS.gene070877.t1 MTR_3g089977 24.074 378 243 14 12 355 12 379 9.48e-16 78.2
MS.gene070877.t1 MTR_6g465010 27.700 213 138 6 26 234 13 213 1.21e-15 77.4
MS.gene070877.t1 MTR_6g466320 28.169 213 137 7 26 234 29 229 1.65e-15 77.4
MS.gene070877.t1 MTR_6g465010 28.169 213 137 7 26 234 29 229 1.65e-15 77.4
MS.gene070877.t1 MTR_3g089940 24.457 368 235 13 12 345 12 370 1.80e-15 77.4
MS.gene070877.t1 MTR_3g089940 24.011 379 245 13 1 345 1 370 3.09e-15 76.6
MS.gene070877.t1 MTR_3g089940 25.856 263 168 7 12 254 12 267 5.70e-15 75.5
MS.gene070877.t1 MTR_7g068320 26.190 336 212 14 40 341 48 381 7.26e-15 75.5
MS.gene070877.t1 MTR_3g089940 25.182 274 178 7 1 254 1 267 7.90e-15 75.1
MS.gene070877.t1 MTR_3g089970 24.044 366 235 12 14 345 15 371 5.51e-14 72.8
MS.gene070877.t1 MTR_5g045710 24.556 338 218 10 39 345 35 366 8.99e-14 72.0
MS.gene070877.t1 MTR_7g099040 25.490 357 223 13 2 328 4 347 2.87e-13 70.9
MS.gene070877.t1 MTR_1g069430 25.882 340 216 13 1 313 1 331 6.94e-13 69.7
MS.gene070877.t1 MTR_7g068330 26.627 338 207 15 40 341 41 373 8.31e-13 69.3
MS.gene070877.t1 MTR_5g031380 64.103 39 14 0 81 119 19 57 2.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070877.t1 AT4G37980 71.067 356 101 1 6 361 3 356 9.26e-175 490
MS.gene070877.t1 AT4G37990 70.225 356 104 1 6 361 3 356 1.19e-170 479
MS.gene070877.t1 AT4G37970 62.890 353 131 0 9 361 9 361 6.98e-164 462
MS.gene070877.t1 AT4G39330 61.364 352 136 0 9 360 8 359 1.60e-160 454
MS.gene070877.t1 AT2G21890 58.739 349 143 1 13 360 6 354 2.72e-148 424
MS.gene070877.t1 AT2G21730 59.429 350 140 2 13 360 6 355 3.21e-148 423
MS.gene070877.t1 AT4G37980 70.134 298 87 1 6 303 3 298 1.34e-135 389
MS.gene070877.t1 AT4G39330 61.149 296 115 0 9 304 8 303 1.37e-129 374
MS.gene070877.t1 AT4G37970 61.034 290 113 0 9 298 9 298 5.18e-126 364
MS.gene070877.t1 AT3G19450 52.890 346 163 0 12 357 9 354 5.91e-116 341
MS.gene070877.t1 AT1G72680 49.854 343 171 1 17 359 14 355 1.39e-113 335
MS.gene070877.t1 AT4G34230 51.149 348 170 0 7 354 3 350 3.60e-107 318
MS.gene070877.t1 AT4G34230 50.862 348 171 0 7 354 3 350 3.31e-106 316
MS.gene070877.t1 AT4G37970 59.916 237 95 0 9 245 9 245 1.28e-99 295
MS.gene070877.t1 AT4G37970 60.426 235 93 0 9 243 9 243 3.01e-99 295
MS.gene070877.t1 AT5G63620 25.862 290 187 6 16 279 58 345 1.34e-22 98.6
MS.gene070877.t1 AT5G63620 25.862 290 187 6 16 279 58 345 1.70e-22 98.2
MS.gene070877.t1 AT5G63620 27.309 249 158 5 16 243 58 304 1.28e-21 94.4
MS.gene070877.t1 AT5G43940 26.273 373 234 13 1 344 1 361 8.16e-18 84.3
MS.gene070877.t1 AT5G43940 26.347 334 209 10 40 344 48 373 1.72e-17 83.6
MS.gene070877.t1 AT1G22430 25.868 317 203 10 40 328 42 354 3.67e-17 82.4
MS.gene070877.t1 AT1G22430 25.868 317 203 10 40 328 42 354 3.67e-17 82.4
MS.gene070877.t1 AT1G32780 27.072 362 217 15 40 359 37 393 5.87e-16 79.0
MS.gene070877.t1 AT5G51970 25.490 255 169 6 37 279 40 285 6.22e-16 78.6
MS.gene070877.t1 AT5G51970 25.490 255 169 6 37 279 40 285 6.22e-16 78.6
MS.gene070877.t1 AT1G22430 26.000 200 129 5 40 222 42 239 3.75e-13 69.7
MS.gene070877.t1 AT1G22430 26.000 200 129 5 40 222 42 239 5.73e-13 68.9
MS.gene070877.t1 AT1G22430 26.000 200 129 5 40 222 42 239 5.73e-13 68.9
MS.gene070877.t1 AT1G77120 22.539 386 260 12 1 355 1 378 1.44e-12 68.6

Find 93 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGTCCTCTTCGACATTT+TGG 0.218835 5.4:-21448103 MS.gene070877:CDS
AAACCAATCAATGGTAAAAT+AGG 0.229602 5.4:+21447751 None:intergenic
TATCGATTCGTGATTGATAT+TGG 0.245698 5.4:-21447363 MS.gene070877:CDS
GTGAAATTCGCCAAAGCTTT+TGG 0.268679 5.4:-21448019 MS.gene070877:CDS
ATTGGTTTATTGAAATCTAA+TGG 0.271827 5.4:-21447736 MS.gene070877:CDS
AAGTTTGATTGGAGGGATAA+AGG 0.302889 5.4:-21447502 MS.gene070877:CDS
TCTTGGTGGACTTGGTCATA+TGG 0.309264 5.4:-21448044 MS.gene070877:CDS
ACCACATGTGACTGTTTGTT+GGG 0.314826 5.4:+21448264 None:intergenic
CCCATTCATTCTTCATCATA+TGG 0.323377 5.4:+21448674 None:intergenic
TCCTGCAATGTTTAATGGCT+TGG 0.335171 5.4:+21447327 None:intergenic
TTAGTAGCTGGAAGTTTGAT+TGG 0.337701 5.4:-21447513 MS.gene070877:CDS
CATCAAAACTTTAGGGAAAC+TGG 0.347590 5.4:-21448751 MS.gene070877:intron
AAAGTAGAAAAGTTTAAAGT+TGG 0.357138 5.4:-21448367 MS.gene070877:CDS
GCCCAACCAAAGGCCTTCTT+AGG 0.360454 5.4:+21448966 None:intergenic
CACCACATGTGACTGTTTGT+TGG 0.363970 5.4:+21448263 None:intergenic
CCCTGGAACTAATGGATAGA+TGG 0.366233 5.4:+21448645 None:intergenic
ATAGGAACAACCTCAATATC+AGG 0.379793 5.4:+21447435 None:intergenic
AAATGTACCTGCATCTGATC+TGG 0.383383 5.4:+21447905 None:intergenic
TCCATCTATCCATTAGTTCC+AGG 0.385414 5.4:-21448646 MS.gene070877:CDS
AACTTACCTCTTGATGTTGC+CGG 0.388597 5.4:-21448154 MS.gene070877:CDS
TCCTAAGAAGGCCTTTGGTT+GGG 0.390941 5.4:-21448967 MS.gene070877:CDS
ACATCAAGAGGTAAGTTATC+AGG 0.391560 5.4:+21448160 None:intergenic
CGTGATGGCACCGTCACATA+TGG 0.395744 5.4:-21448232 MS.gene070877:CDS
TGGTATTATTGACACAGTTT+CGG 0.397040 5.4:-21447782 MS.gene070877:CDS
GAGCATCCTAAGAAGGCCTT+TGG 0.400649 5.4:-21448972 MS.gene070877:CDS
GCCGGTCCTCTTCTTTGCGC+TGG 0.408424 5.4:-21448136 MS.gene070877:CDS
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.4:+21448009 None:intergenic
AACCTTCTGGAGAAATTGTA+AGG 0.410596 5.4:+21448915 None:intergenic
AAATGCAGGCATGAACTTGC+GGG 0.418681 5.4:-21448412 MS.gene070877:intron
ATAGGCGTTGTTGGTCTTGG+TGG 0.424569 5.4:-21448058 MS.gene070877:CDS
GAAGCATACCCTGGAACTAA+TGG 0.426302 5.4:+21448637 None:intergenic
GTTGTTGGTCTTGGTGGACT+TGG 0.427259 5.4:-21448052 MS.gene070877:CDS
TCCATATGATGAAGAATGAA+TGG 0.431554 5.4:-21448675 MS.gene070877:CDS
GTAGGGAGAAAGTTAGTAGC+TGG 0.438913 5.4:-21447525 MS.gene070877:intron
CATTCAAGGTGTTGTATTGT+GGG 0.442854 5.4:-21448714 MS.gene070877:CDS
TTTAAAGTTGGAGACAAAGT+AGG 0.443860 5.4:-21448355 MS.gene070877:CDS
AATATAGGCGTTGTTGGTCT+TGG 0.444452 5.4:-21448061 MS.gene070877:CDS
GACGGTGCCATCACGATACT+TGG 0.453032 5.4:+21448240 None:intergenic
TCTTCTTTGCGCTGGAGTCA+CGG 0.463575 5.4:-21448128 MS.gene070877:CDS
TGAGAAAGATGTAGCATTCA+AGG 0.467351 5.4:-21448728 MS.gene070877:CDS
TTCTACTTTGCTTCCAACCT+CGG 0.470004 5.4:+21448381 None:intergenic
GCTGTGTTGACATAATCAAT+AGG 0.473733 5.4:+21447417 None:intergenic
CCATATGATGAAGAATGAAT+GGG 0.478005 5.4:-21448674 MS.gene070877:CDS
CAAAGTAGGTGTGGGATACA+TGG 0.478011 5.4:-21448341 MS.gene070877:CDS
CCCCTTACAATTTCTCCAGA+AGG 0.479664 5.4:-21448917 MS.gene070877:intron
GATTTCAATAAACCAATCAA+TGG 0.487235 5.4:+21447742 None:intergenic
GGTATGAATATAGGCGTTGT+TGG 0.488041 5.4:-21448067 MS.gene070877:CDS
CAATGTAAAGCCTGATATTG+AGG 0.489359 5.4:-21447445 MS.gene070877:CDS
TGGAGGTTATTCTGACTCAA+TGG 0.494148 5.4:-21448212 MS.gene070877:CDS
TGGGCAGCAAGAGATCCTTC+TGG 0.496931 5.4:-21448948 MS.gene070877:CDS
TAGCTGGAAGTTTGATTGGA+GGG 0.499663 5.4:-21447509 MS.gene070877:CDS
ATCCTAAGAAGGCCTTTGGT+TGG 0.502470 5.4:-21448968 MS.gene070877:CDS
GACAAGCCTGGTATGAATAT+AGG 0.507608 5.4:-21448076 MS.gene070877:CDS
CCTCCAAGTAAAGAAAATAC+AGG 0.509432 5.4:+21447670 None:intergenic
GCATTCAAGGTGTTGTATTG+TGG 0.522756 5.4:-21448715 MS.gene070877:CDS
GTAGCTGGAAGTTTGATTGG+AGG 0.522925 5.4:-21447510 MS.gene070877:CDS
AGTGTTAGAGCATCCTAAGA+AGG 0.523098 5.4:-21448979 MS.gene070877:CDS
AGTTGGAGACAAAGTAGGTG+TGG 0.528716 5.4:-21448350 MS.gene070877:CDS
TACATCGCCTAACAAAGAAA+AGG 0.540053 5.4:-21447975 MS.gene070877:CDS
CCACATGTGACTGTTTGTTG+GGG 0.540150 5.4:+21448265 None:intergenic
GAAAATACAGGAAGCTCTAG+AGG 0.546279 5.4:+21447682 None:intergenic
ATCTCTTGCTGCCCAACCAA+AGG 0.547740 5.4:+21448956 None:intergenic
CCATCTATCCATTAGTTCCA+GGG 0.551121 5.4:-21448645 MS.gene070877:intron
GGAAAGCTTGTAATGGTTGG+TGG 0.563386 5.4:-21447715 MS.gene070877:CDS
TCCAGCGCAAAGAAGAGGAC+CGG 0.564917 5.4:+21448135 None:intergenic
GCTGCAATGAGTACTTTGAA+TGG 0.568095 5.4:-21447802 MS.gene070877:CDS
TAAATGCAGGCATGAACTTG+CGG 0.570766 5.4:-21448413 MS.gene070877:intron
AGCAATAGAACACGTGGGAG+CGG 0.573972 5.4:-21447951 MS.gene070877:CDS
GTGACTCCAGCGCAAAGAAG+AGG 0.576992 5.4:+21448130 None:intergenic
GTTGGAGACAAAGTAGGTGT+GGG 0.583586 5.4:-21448349 MS.gene070877:CDS
AATGTACCTGCATCTGATCT+GGG 0.586593 5.4:+21447906 None:intergenic
ATCTAATGGAAAGCTTGTAA+TGG 0.587781 5.4:-21447722 MS.gene070877:CDS
ACCTTCTGGAGAAATTGTAA+GGG 0.594703 5.4:+21448916 None:intergenic
GCTTGTAATGGTTGGTGGAG+TGG 0.601696 5.4:-21447710 MS.gene070877:CDS
CCTGCATCTGATCTGGGTCG+CGG 0.601874 5.4:+21447912 None:intergenic
CCGCGACCCAGATCAGATGC+AGG 0.603268 5.4:-21447912 MS.gene070877:intron
TGATTATGTCAACACAGCAA+TGG 0.603631 5.4:-21447412 MS.gene070877:CDS
GCGGGTATAGTAACCGAGGT+TGG 0.608625 5.4:-21448394 MS.gene070877:CDS
AGAGGACCGGCAACATCAAG+AGG 0.610980 5.4:+21448148 None:intergenic
AATGGAAAGCTTGTAATGGT+TGG 0.618001 5.4:-21447718 MS.gene070877:CDS
CCTTCTGGAGAAATTGTAAG+GGG 0.623079 5.4:+21448917 None:intergenic
TCGAGTCCAAAATGTCGAAG+AGG 0.639224 5.4:+21448097 None:intergenic
GATGGCACCGTCACATATGG+AGG 0.644175 5.4:-21448229 MS.gene070877:CDS
ACAACGCCTATATTCATACC+AGG 0.648632 5.4:+21448070 None:intergenic
AAAGGAAGCAATAGAACACG+TGG 0.649929 5.4:-21447957 MS.gene070877:CDS
AGAATAACCTCCATATGTGA+CGG 0.659885 5.4:+21448222 None:intergenic
CACATGTGACTGTTTGTTGG+GGG 0.661508 5.4:+21448266 None:intergenic
CATATGATGAAGAATGAATG+GGG 0.667164 5.4:-21448673 MS.gene070877:CDS
TAAGGGGAGAGAACACCAGA+AGG 0.676012 5.4:+21448933 None:intergenic
CCCCAACAAACAGTCACATG+TGG 0.678792 5.4:-21448265 MS.gene070877:CDS
TGTGGTGCCAAGTATCGTGA+TGG 0.704207 5.4:-21448247 MS.gene070877:CDS
AAGGAAGCAATAGAACACGT+GGG 0.744714 5.4:-21447956 MS.gene070877:CDS
ACTTGCGGGTATAGTAACCG+AGG 0.754285 5.4:-21448398 MS.gene070877:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTATTTAATAAATGC+AGG - chr5.4:21447908-21447927 MS.gene070877:intron 10.0%
!! ATTAATTTCACATTATTTGT+AGG - chr5.4:21448791-21448810 MS.gene070877:intron 15.0%
!! TTAAAGTAAGTATAAAGATA+TGG + chr5.4:21447768-21447787 None:intergenic 15.0%
!! TTAATTTCACATTATTTGTA+GGG - chr5.4:21448792-21448811 MS.gene070877:intron 15.0%
!!! ATATGGAAATATTTTTCATA+TGG + chr5.4:21448693-21448712 None:intergenic 15.0%
!!! ATCAATTCATCTTTATTTAA+TGG - chr5.4:21447452-21447471 MS.gene070877:CDS 15.0%
!!! TCAATTCATCTTTATTTAAT+GGG - chr5.4:21447453-21447472 MS.gene070877:CDS 15.0%
!! TAAACTATAATTGTAGTAGA+AGG + chr5.4:21447728-21447747 None:intergenic 20.0%
!!! AAAGTAGAAAAGTTTAAAGT+TGG - chr5.4:21447967-21447986 MS.gene070877:CDS 20.0%
!!! ATTATTTTAGAAATTGGCAA+TGG - chr5.4:21448474-21448493 MS.gene070877:intron 20.0%
!!! ATTGGTTTATTGAAATCTAA+TGG - chr5.4:21448598-21448617 MS.gene070877:intron 20.0%
!!! TTAGTCTTAAACTATAGTAT+GGG + chr5.4:21448721-21448740 None:intergenic 20.0%
!!! TTTAGTCTTAAACTATAGTA+TGG + chr5.4:21448722-21448741 None:intergenic 20.0%
! AAACCAATCAATGGTAAAAT+AGG + chr5.4:21448586-21448605 None:intergenic 25.0%
! GATTTCAATAAACCAATCAA+TGG + chr5.4:21448595-21448614 None:intergenic 25.0%
! GCTACTAATTAATTCAACTT+TGG - chr5.4:21447558-21447577 MS.gene070877:intron 25.0%
! TTAATTTCGATCTATGTTGT+TGG - chr5.4:21447536-21447555 MS.gene070877:intron 25.0%
!! AATACTGTGCAACATTTTAT+AGG - chr5.4:21448510-21448529 MS.gene070877:intron 25.0%
!!! TCAAGAAATGATTGATTTTG+CGG - chr5.4:21448859-21448878 MS.gene070877:intron 25.0%
ATAATTGTAGTAGAAGGAGT+TGG + chr5.4:21447722-21447741 None:intergenic 30.0%
ATCTAATGGAAAGCTTGTAA+TGG - chr5.4:21448612-21448631 MS.gene070877:intron 30.0%
CATATGATGAAGAATGAATG+GGG - chr5.4:21447661-21447680 MS.gene070877:intron 30.0%
CCATATGATGAAGAATGAAT+GGG - chr5.4:21447660-21447679 MS.gene070877:intron 30.0%
GATTAATGAAGTAAACCTTC+TGG + chr5.4:21447435-21447454 None:intergenic 30.0%
TAGTTTATTCCTACGTTACT+TGG - chr5.4:21447741-21447760 MS.gene070877:CDS 30.0%
TCCATATGATGAAGAATGAA+TGG - chr5.4:21447659-21447678 MS.gene070877:intron 30.0%
TGGTATTATTGACACAGTTT+CGG - chr5.4:21448552-21448571 MS.gene070877:intron 30.0%
! ATTGCTTCCTTTTCTTTGTT+AGG + chr5.4:21448369-21448388 None:intergenic 30.0%
! CATCCTATTTTACCATTGAT+TGG - chr5.4:21448580-21448599 MS.gene070877:intron 30.0%
! CTATAGTATGGGAGTATATA+TGG + chr5.4:21448710-21448729 None:intergenic 30.0%
! CTTCCTGTATTTTCTTTACT+TGG - chr5.4:21448661-21448680 MS.gene070877:CDS 30.0%
! GTTGCACAGTATTAGTTTAT+TGG + chr5.4:21448503-21448522 None:intergenic 30.0%
! TTTAAAGTTGGAGACAAAGT+AGG - chr5.4:21447979-21447998 MS.gene070877:CDS 30.0%
! TTTGGTGCATCAAAACTTTA+GGG - chr5.4:21447576-21447595 MS.gene070877:intron 30.0%
!! TATCGATTCGTGATTGATAT+TGG - chr5.4:21448971-21448990 MS.gene070877:CDS 30.0%
AAGTTTGATTGGAGGGATAA+AGG - chr5.4:21448832-21448851 MS.gene070877:intron 35.0%
AATGGAAAGCTTGTAATGGT+TGG - chr5.4:21448616-21448635 MS.gene070877:intron 35.0%
ACATCAAGAGGTAAGTTATC+AGG + chr5.4:21448177-21448196 None:intergenic 35.0%
AGAATAACCTCCATATGTGA+CGG + chr5.4:21448115-21448134 None:intergenic 35.0%
ATAGGAACAACCTCAATATC+AGG + chr5.4:21448902-21448921 None:intergenic 35.0%
CAATGTAAAGCCTGATATTG+AGG - chr5.4:21448889-21448908 MS.gene070877:intron 35.0%
CCCATTCATTCTTCATCATA+TGG + chr5.4:21447663-21447682 None:intergenic 35.0%
CCTCCAAGTAAAGAAAATAC+AGG + chr5.4:21448667-21448686 None:intergenic 35.0%
TACATCGCCTAACAAAGAAA+AGG - chr5.4:21448359-21448378 MS.gene070877:CDS 35.0%
TGAGAAAGATGTAGCATTCA+AGG - chr5.4:21447606-21447625 MS.gene070877:intron 35.0%
TGATTATGTCAACACAGCAA+TGG - chr5.4:21448922-21448941 MS.gene070877:CDS 35.0%
TTAGTAGCTGGAAGTTTGAT+TGG - chr5.4:21448821-21448840 MS.gene070877:intron 35.0%
! AACCTTCTGGAGAAATTGTA+AGG + chr5.4:21447422-21447441 None:intergenic 35.0%
! ACCTTCTGGAGAAATTGTAA+GGG + chr5.4:21447421-21447440 None:intergenic 35.0%
! CATCAAAACTTTAGGGAAAC+TGG - chr5.4:21447583-21447602 MS.gene070877:intron 35.0%
! CCTGTATTTTCTTTACTTGG+AGG - chr5.4:21448664-21448683 MS.gene070877:CDS 35.0%
! CTTTGGTGCATCAAAACTTT+AGG - chr5.4:21447575-21447594 MS.gene070877:intron 35.0%
! GCTGTGTTGACATAATCAAT+AGG + chr5.4:21448920-21448939 None:intergenic 35.0%
! TATAGTCCTCTTCGACATTT+TGG - chr5.4:21448231-21448250 MS.gene070877:CDS 35.0%
!! CATTCAAGGTGTTGTATTGT+GGG - chr5.4:21447620-21447639 MS.gene070877:intron 35.0%
AAAGGAAGCAATAGAACACG+TGG - chr5.4:21448377-21448396 MS.gene070877:CDS 40.0%
AAATGTACCTGCATCTGATC+TGG + chr5.4:21448432-21448451 None:intergenic 40.0%
AACTTACCTCTTGATGTTGC+CGG - chr5.4:21448180-21448199 MS.gene070877:CDS 40.0%
AAGGAAGCAATAGAACACGT+GGG - chr5.4:21448378-21448397 MS.gene070877:CDS 40.0%
AATGTACCTGCATCTGATCT+GGG + chr5.4:21448431-21448450 None:intergenic 40.0%
ACAACGCCTATATTCATACC+AGG + chr5.4:21448267-21448286 None:intergenic 40.0%
ACCACATGTGACTGTTTGTT+GGG + chr5.4:21448073-21448092 None:intergenic 40.0%
AGATATGGTCCAAGTAACGT+AGG + chr5.4:21447753-21447772 None:intergenic 40.0%
CACATTAGCACCAAAAGCTT+TGG + chr5.4:21448328-21448347 None:intergenic 40.0%
CCATCTATCCATTAGTTCCA+GGG - chr5.4:21447689-21447708 MS.gene070877:CDS 40.0%
GACAAGCCTGGTATGAATAT+AGG - chr5.4:21448258-21448277 MS.gene070877:CDS 40.0%
GCTGCAATGAGTACTTTGAA+TGG - chr5.4:21448532-21448551 MS.gene070877:intron 40.0%
TAAATGCAGGCATGAACTTG+CGG - chr5.4:21447921-21447940 MS.gene070877:CDS 40.0%
TAGCTGGAAGTTTGATTGGA+GGG - chr5.4:21448825-21448844 MS.gene070877:intron 40.0%
TCCATCTATCCATTAGTTCC+AGG - chr5.4:21447688-21447707 MS.gene070877:CDS 40.0%
TGGAGGTTATTCTGACTCAA+TGG - chr5.4:21448122-21448141 MS.gene070877:CDS 40.0%
TTCTACTTTGCTTCCAACCT+CGG + chr5.4:21447956-21447975 None:intergenic 40.0%
! AATATAGGCGTTGTTGGTCT+TGG - chr5.4:21448273-21448292 MS.gene070877:CDS 40.0%
! AGTGTTAGAGCATCCTAAGA+AGG - chr5.4:21447355-21447374 MS.gene070877:CDS 40.0%
! CAGCACAATTTTCGCATGAT+CGG + chr5.4:21448029-21448048 None:intergenic 40.0%
! CCTTCTGGAGAAATTGTAAG+GGG + chr5.4:21447420-21447439 None:intergenic 40.0%
! GAAAATACAGGAAGCTCTAG+AGG + chr5.4:21448655-21448674 None:intergenic 40.0%
! GGTATGAATATAGGCGTTGT+TGG - chr5.4:21448267-21448286 MS.gene070877:CDS 40.0%
! GTGAAATTCGCCAAAGCTTT+TGG - chr5.4:21448315-21448334 MS.gene070877:CDS 40.0%
!! GCATTCAAGGTGTTGTATTG+TGG - chr5.4:21447619-21447638 MS.gene070877:intron 40.0%
AAATGCAGGCATGAACTTGC+GGG - chr5.4:21447922-21447941 MS.gene070877:CDS 45.0%
AGTTGGAGACAAAGTAGGTG+TGG - chr5.4:21447984-21448003 MS.gene070877:CDS 45.0%
ATCCTAAGAAGGCCTTTGGT+TGG - chr5.4:21447366-21447385 MS.gene070877:CDS 45.0%
CAAAGTAGGTGTGGGATACA+TGG - chr5.4:21447993-21448012 MS.gene070877:CDS 45.0%
CACCACATGTGACTGTTTGT+TGG + chr5.4:21448074-21448093 None:intergenic 45.0%
CCCCTTACAATTTCTCCAGA+AGG - chr5.4:21447417-21447436 MS.gene070877:CDS 45.0%
GAAGCATACCCTGGAACTAA+TGG + chr5.4:21447700-21447719 None:intergenic 45.0%
GGAAAGCTTGTAATGGTTGG+TGG - chr5.4:21448619-21448638 MS.gene070877:intron 45.0%
GTAGCTGGAAGTTTGATTGG+AGG - chr5.4:21448824-21448843 MS.gene070877:intron 45.0%
GTAGGGAGAAAGTTAGTAGC+TGG - chr5.4:21448809-21448828 MS.gene070877:intron 45.0%
GTTGGAGACAAAGTAGGTGT+GGG - chr5.4:21447985-21448004 MS.gene070877:CDS 45.0%
TCCTAAGAAGGCCTTTGGTT+GGG - chr5.4:21447367-21447386 MS.gene070877:CDS 45.0%
TCGAGTCCAAAATGTCGAAG+AGG + chr5.4:21448240-21448259 None:intergenic 45.0%
! CACATGTGACTGTTTGTTGG+GGG + chr5.4:21448071-21448090 None:intergenic 45.0%
! CCACATGTGACTGTTTGTTG+GGG + chr5.4:21448072-21448091 None:intergenic 45.0%
! CCCTGGAACTAATGGATAGA+TGG + chr5.4:21447692-21447711 None:intergenic 45.0%
!! TCTTGGTGGACTTGGTCATA+TGG - chr5.4:21448290-21448309 MS.gene070877:CDS 45.0%
!!! AATTTAATTATTTTAGAAAT+TGG - chr5.4:21448468-21448487 MS.gene070877:intron 5.0%
ACTTGCGGGTATAGTAACCG+AGG - chr5.4:21447936-21447955 MS.gene070877:CDS 50.0%
AGCAATAGAACACGTGGGAG+CGG - chr5.4:21448383-21448402 MS.gene070877:CDS 50.0%
ATCTCTTGCTGCCCAACCAA+AGG + chr5.4:21447381-21447400 None:intergenic 50.0%
CCCCAACAAACAGTCACATG+TGG - chr5.4:21448069-21448088 MS.gene070877:CDS 50.0%
GAGCATCCTAAGAAGGCCTT+TGG - chr5.4:21447362-21447381 MS.gene070877:CDS 50.0%
TAAGGGGAGAGAACACCAGA+AGG + chr5.4:21447404-21447423 None:intergenic 50.0%
TCTTCTTTGCGCTGGAGTCA+CGG - chr5.4:21448206-21448225 MS.gene070877:CDS 50.0%
! AAGGAGTTGGAAGCATACCC+TGG + chr5.4:21447709-21447728 None:intergenic 50.0%
! CATTTTGGACTCGACAAGCC+TGG - chr5.4:21448246-21448265 MS.gene070877:CDS 50.0%
! TGTGGTGCCAAGTATCGTGA+TGG - chr5.4:21448087-21448106 MS.gene070877:CDS 50.0%
!! ATAGGCGTTGTTGGTCTTGG+TGG - chr5.4:21448276-21448295 MS.gene070877:CDS 50.0%
!! GCTTGTAATGGTTGGTGGAG+TGG - chr5.4:21448624-21448643 MS.gene070877:intron 50.0%
!! GTTGTTGGTCTTGGTGGACT+TGG - chr5.4:21448282-21448301 MS.gene070877:CDS 50.0%
AGAGGACCGGCAACATCAAG+AGG + chr5.4:21448189-21448208 None:intergenic 55.0%
CGTGATGGCACCGTCACATA+TGG - chr5.4:21448102-21448121 MS.gene070877:CDS 55.0%
GATGGCACCGTCACATATGG+AGG - chr5.4:21448105-21448124 MS.gene070877:CDS 55.0%
GCCCAACCAAAGGCCTTCTT+AGG + chr5.4:21447371-21447390 None:intergenic 55.0%
GCGGGTATAGTAACCGAGGT+TGG - chr5.4:21447940-21447959 MS.gene070877:CDS 55.0%
GTGACTCCAGCGCAAAGAAG+AGG + chr5.4:21448207-21448226 None:intergenic 55.0%
TCCAGCGCAAAGAAGAGGAC+CGG + chr5.4:21448202-21448221 None:intergenic 55.0%
TGGGCAGCAAGAGATCCTTC+TGG - chr5.4:21447386-21447405 MS.gene070877:CDS 55.0%
!! GACGGTGCCATCACGATACT+TGG + chr5.4:21448097-21448116 None:intergenic 55.0%
CCTGCATCTGATCTGGGTCG+CGG + chr5.4:21448425-21448444 None:intergenic 60.0%
CCGCGACCCAGATCAGATGC+AGG - chr5.4:21448422-21448441 MS.gene070877:intron 65.0%
GCCGGTCCTCTTCTTTGCGC+TGG - chr5.4:21448198-21448217 MS.gene070877:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 21447338 21449018 21447338 ID=MS.gene070877
chr5.4 mRNA 21447338 21449018 21447338 ID=MS.gene070877.t1;Parent=MS.gene070877
chr5.4 exon 21448918 21449018 21448918 ID=MS.gene070877.t1.exon1;Parent=MS.gene070877.t1
chr5.4 CDS 21448918 21449018 21448918 ID=cds.MS.gene070877.t1;Parent=MS.gene070877.t1
chr5.4 exon 21448646 21448759 21448646 ID=MS.gene070877.t1.exon2;Parent=MS.gene070877.t1
chr5.4 CDS 21448646 21448759 21448646 ID=cds.MS.gene070877.t1;Parent=MS.gene070877.t1
chr5.4 exon 21447913 21448426 21447913 ID=MS.gene070877.t1.exon3;Parent=MS.gene070877.t1
chr5.4 CDS 21447913 21448426 21447913 ID=cds.MS.gene070877.t1;Parent=MS.gene070877.t1
chr5.4 exon 21447671 21447824 21447671 ID=MS.gene070877.t1.exon4;Parent=MS.gene070877.t1
chr5.4 CDS 21447671 21447824 21447671 ID=cds.MS.gene070877.t1;Parent=MS.gene070877.t1
chr5.4 exon 21447338 21447543 21447338 ID=MS.gene070877.t1.exon5;Parent=MS.gene070877.t1
chr5.4 CDS 21447338 21447543 21447338 ID=cds.MS.gene070877.t1;Parent=MS.gene070877.t1
Gene Sequence

>MS.gene070877

ATGGCAACAGAAACTAAAGTGTTAGAGCATCCTAAGAAGGCCTTTGGTTGGGCAGCAAGAGATCCTTCTGGTGTTCTCTCCCCTTACAATTTCTCCAGAAGGTTTACTTCATTAATCAATTCATCTTTATTTAATGGGATGTTTTAATTTAATTAATATATATTTGATGTTTAGATATATGTATATGTTTTTTTTTACTTAATTTCGATCTATGTTGTTGGCTACTAATTAATTCAACTTTGGTGCATCAAAACTTTAGGGAAACTGGTGAGAAAGATGTAGCATTCAAGGTGTTGTATTGTGGGATATGTCACACTGATCTCCATATGATGAAGAATGAATGGGGCAATTCCATCTATCCATTAGTTCCAGGGTATGCTTCCAACTCCTTCTACTACAATTATAGTTTATTCCTACGTTACTTGGACCATATCTTTATACTTACTTTAATGATAAAAGAGTAAAAAGTAAGAGTATATTTAAAATTTGCTTATAATTAATTAAAAATAATTTTTAATTTGTACAAAATAATCTTAAGAAATGAGTAGTAAATAATTGTTCAATTCGTTTATAATTTATTTAATAAATGCAGGCATGAACTTGCGGGTATAGTAACCGAGGTTGGAAGCAAAGTAGAAAAGTTTAAAGTTGGAGACAAAGTAGGTGTGGGATACATGGTTGATTCATGCCGATCATGCGAAAATTGTGCTGACGATCTTGAGAATTACTGCCCCCAACAAACAGTCACATGTGGTGCCAAGTATCGTGATGGCACCGTCACATATGGAGGTTATTCTGACTCAATGGTTGCAGACGAGCACTTTGTGATTCGCATTCCTGATAACTTACCTCTTGATGTTGCCGGTCCTCTTCTTTGCGCTGGAGTCACGGTGTATAGTCCTCTTCGACATTTTGGACTCGACAAGCCTGGTATGAATATAGGCGTTGTTGGTCTTGGTGGACTTGGTCATATGGCTGTGAAATTCGCCAAAGCTTTTGGTGCTAATGTGACTGTAATTAGTACATCGCCTAACAAAGAAAAGGAAGCAATAGAACACGTGGGAGCGGACTCGTTTCTAGTAAGCCGCGACCCAGATCAGATGCAGGTACATTTCATTTGATCAATTGTGAATTTAATTATTTTAGAAATTGGCAATGGCAACCAATAAACTAATACTGTGCAACATTTTATAGGCTGCAATGAGTACTTTGAATGGTATTATTGACACAGTTTCGGCGAGTCATCCTATTTTACCATTGATTGGTTTATTGAAATCTAATGGAAAGCTTGTAATGGTTGGTGGAGTGGCAAAGCCTCTAGAGCTTCCTGTATTTTCTTTACTTGGAGGTAACCATATGAAAAATATTTCCATATATACTCCCATACTATAGTTTAAGACTAAATGCTAAATATGCAGTTTTAATTCAAACTATGCAGAATGTTGATACTTATATTAATTTCACATTATTTGTAGGGAGAAAGTTAGTAGCTGGAAGTTTGATTGGAGGGATAAAGGAGACTCAAGAAATGATTGATTTTGCGGCAGAACACAATGTAAAGCCTGATATTGAGGTTGTTCCTATTGATTATGTCAACACAGCAATGGAGCGTCTCGAGAAAGCAGACGTGAAATATCGATTCGTGATTGATATTGGAAATACGCTGAAACCAAGCCATTAA
Protein sequence

>MS.gene070877.t1

MATETKVLEHPKKAFGWAARDPSGVLSPYNFSRRETGEKDVAFKVLYCGICHTDLHMMKNEWGNSIYPLVPGHELAGIVTEVGSKVEKFKVGDKVGVGYMVDSCRSCENCADDLENYCPQQTVTCGAKYRDGTVTYGGYSDSMVADEHFVIRIPDNLPLDVAGPLLCAGVTVYSPLRHFGLDKPGMNIGVVGLGGLGHMAVKFAKAFGANVTVISTSPNKEKEAIEHVGADSFLVSRDPDQMQAAMSTLNGIIDTVSASHPILPLIGLLKSNGKLVMVGGVAKPLELPVFSLLGGRKLVAGSLIGGIKETQEMIDFAAEHNVKPDIEVVPIDYVNTAMERLEKADVKYRFVIDIGNTLKPSH