AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068620.t1 MTR_5g031190 97.230 361 10 0 1 361 1 361 0.0 724
MS.gene068620.t1 MTR_5g031180 86.427 361 49 0 1 361 1 361 0.0 609
MS.gene068620.t1 MTR_8g068190 80.833 360 69 0 1 360 1 360 0.0 578
MS.gene068620.t1 MTR_5g031250 77.839 361 80 0 1 361 1 361 0.0 576
MS.gene068620.t1 MTR_5g031390 76.454 361 85 0 1 361 1 361 0.0 571
MS.gene068620.t1 MTR_5g031230 75.900 361 87 0 1 361 1 361 0.0 569
MS.gene068620.t1 MTR_4g115300 75.069 361 90 0 1 361 1 361 0.0 563
MS.gene068620.t1 MTR_5g031310 76.271 354 84 0 7 360 8 361 0.0 560
MS.gene068620.t1 MTR_5g031360 76.068 351 84 0 10 360 12 362 0.0 557
MS.gene068620.t1 MTR_5g031305 74.792 361 90 1 1 360 1 361 0.0 554
MS.gene068620.t1 MTR_5g031300 72.676 355 97 0 7 361 8 362 0.0 541
MS.gene068620.t1 MTR_5g031210 74.515 361 92 0 1 361 1 361 0.0 541
MS.gene068620.t1 MTR_8g092630 64.146 357 122 2 8 359 60 415 3.45e-152 435
MS.gene068620.t1 MTR_5g031430 62.029 345 131 0 14 358 14 358 1.30e-147 421
MS.gene068620.t1 MTR_1g025950 57.382 359 152 1 3 360 2 360 1.78e-138 398
MS.gene068620.t1 MTR_5g031250 76.763 241 56 0 1 241 1 241 3.54e-134 383
MS.gene068620.t1 MTR_1g107425 52.770 343 162 0 11 353 9 351 4.12e-124 361
MS.gene068620.t1 MTR_2g009810 54.046 346 157 2 14 358 62 406 5.88e-123 360
MS.gene068620.t1 MTR_1g107425 52.083 288 138 0 11 298 9 296 1.60e-98 294
MS.gene068620.t1 MTR_2g009820 50.800 250 113 4 116 358 8 254 3.13e-69 218
MS.gene068620.t1 MTR_5g031290 73.684 114 30 0 247 360 80 193 1.80e-54 177
MS.gene068620.t1 MTR_5g031290 80.000 80 15 1 1 79 1 80 5.74e-41 142
MS.gene068620.t1 MTR_8g095220 27.143 280 174 7 25 278 68 343 8.35e-24 102
MS.gene068620.t1 MTR_8g095220 28.512 242 148 6 25 245 68 305 3.01e-23 99.0
MS.gene068620.t1 MTR_3g089940 24.044 366 239 14 11 344 12 370 7.40e-18 84.3
MS.gene068620.t1 MTR_3g089977 23.467 375 249 13 11 354 12 379 7.69e-18 84.3
MS.gene068620.t1 MTR_3g089940 23.964 338 221 12 39 344 37 370 1.50e-17 83.6
MS.gene068620.t1 MTR_0001s0660 25.828 302 200 9 38 318 35 333 4.38e-17 82.8
MS.gene068620.t1 MTR_3g089940 24.251 334 222 12 11 319 12 339 5.28e-16 78.6
MS.gene068620.t1 MTR_3g089970 23.373 338 223 12 39 344 38 371 6.27e-16 78.6
MS.gene068620.t1 MTR_3g089940 23.952 334 223 12 11 319 12 339 9.90e-16 77.8
MS.gene068620.t1 MTR_6g465010 26.224 286 188 8 15 288 3 277 2.56e-15 76.6
MS.gene068620.t1 MTR_6g466320 26.224 286 188 8 15 288 19 293 2.69e-15 76.6
MS.gene068620.t1 MTR_6g465010 26.224 286 188 8 15 288 19 293 2.69e-15 76.6
MS.gene068620.t1 MTR_1g069430 23.739 337 226 9 1 312 1 331 9.92e-14 72.0
MS.gene068620.t1 MTR_7g068320 26.074 349 221 14 39 353 48 393 1.77e-13 71.2
MS.gene068620.t1 MTR_5g045710 24.918 305 200 9 38 318 35 334 3.23e-13 70.5
MS.gene068620.t1 MTR_7g099040 23.659 317 209 8 38 327 37 347 3.21e-12 67.4
MS.gene068620.t1 MTR_3g099460 26.537 309 197 8 39 319 37 343 7.89e-11 63.2
MS.gene068620.t1 MTR_3g099460 26.537 309 197 8 39 319 44 350 9.08e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068620.t1 AT4G37970 66.197 355 120 0 6 360 7 361 2.68e-172 484
MS.gene068620.t1 AT4G37980 71.714 350 99 0 11 360 7 356 4.87e-172 483
MS.gene068620.t1 AT4G37990 72.000 350 98 0 11 360 7 356 1.28e-170 479
MS.gene068620.t1 AT4G39330 62.745 357 133 0 1 357 1 357 5.76e-167 470
MS.gene068620.t1 AT2G21730 61.190 353 135 2 7 357 1 353 1.53e-156 444
MS.gene068620.t1 AT2G21890 60.227 352 139 1 7 357 1 352 7.33e-155 440
MS.gene068620.t1 AT4G39330 61.812 309 118 0 1 309 1 309 6.46e-137 392
MS.gene068620.t1 AT4G37980 72.535 284 78 0 11 294 7 290 3.15e-134 385
MS.gene068620.t1 AT4G37970 65.398 289 100 0 6 294 7 295 6.42e-133 382
MS.gene068620.t1 AT3G19450 53.757 346 160 0 11 356 9 354 6.78e-118 346
MS.gene068620.t1 AT1G72680 48.324 358 182 2 1 358 1 355 3.21e-113 333
MS.gene068620.t1 AT4G34230 53.107 354 165 1 7 359 4 357 2.57e-109 324
MS.gene068620.t1 AT4G34230 52.825 354 166 1 7 359 4 357 2.50e-108 321
MS.gene068620.t1 AT4G37970 64.979 237 83 0 6 242 7 243 2.39e-105 310
MS.gene068620.t1 AT4G37970 64.979 237 83 0 6 242 7 243 2.85e-105 310
MS.gene068620.t1 AT5G63620 27.241 290 183 6 15 278 58 345 1.94e-25 106
MS.gene068620.t1 AT5G63620 27.241 290 183 6 15 278 58 345 2.46e-25 106
MS.gene068620.t1 AT5G63620 28.287 251 157 5 15 244 58 306 3.33e-24 101
MS.gene068620.t1 AT5G51970 26.449 276 180 8 15 278 21 285 3.51e-16 79.3
MS.gene068620.t1 AT5G51970 26.449 276 180 8 15 278 21 285 3.51e-16 79.3
MS.gene068620.t1 AT1G64710 24.516 310 199 10 39 318 13 317 1.06e-15 77.8
MS.gene068620.t1 AT1G64710 24.516 310 199 10 39 318 36 340 1.17e-15 77.8
MS.gene068620.t1 AT5G43940 24.183 306 204 7 39 319 48 350 1.26e-15 77.8
MS.gene068620.t1 AT5G43940 24.183 306 204 7 39 319 36 338 1.38e-15 77.8
MS.gene068620.t1 AT1G77120 23.055 347 232 12 39 354 36 378 2.80e-15 76.6
MS.gene068620.t1 AT1G32780 25.275 364 221 15 39 358 37 393 8.32e-13 69.3

Find 101 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGCCCTATGAAACATTT+TGG 0.212281 5.1:-21445936 MS.gene068620:CDS
CTTGGTCTATTGAAGAATAA+TGG 0.272969 5.1:-21445533 MS.gene068620:CDS
CGCCAAAGCCTTTGGGGTTA+AGG 0.276300 5.1:-21445844 MS.gene068620:CDS
TCAAGTCCAAAATGTTTCAT+AGG 0.278051 5.1:+21445930 None:intergenic
TGAAGTTCGCCAAAGCCTTT+GGG 0.299698 5.1:-21445851 MS.gene068620:CDS
GTTGTTGGTCTTGGTGGATT+AGG 0.305881 5.1:-21445885 MS.gene068620:CDS
GTGAAGTTCGCCAAAGCCTT+TGG 0.307536 5.1:-21445852 MS.gene068620:CDS
GTGATAAGGCATCAGGAATA+TGG 0.344459 5.1:+21446000 None:intergenic
CTCCTTTCAAGTTCTCTAGA+AGG 0.347990 5.1:-21447076 MS.gene068620:intron
GTTGGTGTGGGGTGCATGGT+TGG 0.355897 5.1:-21446170 MS.gene068620:CDS
TACCTTAACCCCAAAGGCTT+TGG 0.358168 5.1:+21445842 None:intergenic
GCTGCTCCTCTCCTTTGTGC+TGG 0.359169 5.1:-21445969 MS.gene068620:CDS
GCTGTAATGGGCACGTTTGA+TGG 0.372955 5.1:-21445599 MS.gene068620:CDS
GGAAACTCCAAAGGCTTCTC+TGG 0.377400 5.1:+21445488 None:intergenic
CACATAGGTGTTGTTGGTCT+TGG 0.390395 5.1:-21445894 MS.gene068620:CDS
CCCACAATCAAAGAAAATAC+TGG 0.392755 5.1:+21445467 None:intergenic
CAATATGTTATAGGCTGTAA+TGG 0.393588 5.1:-21445612 MS.gene068620:intron
GAATAATGGTAAACTTGTTA+TGG 0.398732 5.1:-21445519 MS.gene068620:CDS
GATAGTGCCATCAACATTCT+TGG 0.402024 5.1:+21446073 None:intergenic
CAGGGTTGGTGTGGGGTGCA+TGG 0.404978 5.1:-21446174 MS.gene068620:CDS
ACTGGAACAACCTCAATATC+AGG 0.408861 5.1:+21445272 None:intergenic
AGACCAAGCAAAGGCATTAG+AGG 0.414126 5.1:+21445548 None:intergenic
ACTACTTACCCAGTAGTTCC+TGG 0.414223 5.1:-21446375 MS.gene068620:CDS
ATAGGTGTTGTTGGTCTTGG+TGG 0.418861 5.1:-21445891 MS.gene068620:CDS
ACAATTGGTGGGAGTATGAT+TGG 0.420703 5.1:-21445350 MS.gene068620:CDS
TATTGGTACGCACCTTCATC+TGG 0.421150 5.1:+21445733 None:intergenic
TCTTGGTGGATTAGGTCATA+TGG 0.422443 5.1:-21445877 MS.gene068620:CDS
AATGGCCTCAAAACCAGAAA+TGG 0.429229 5.1:-21447153 MS.gene068620:CDS
TACAGTTCCAAGAATGTTGA+TGG 0.430392 5.1:-21446080 MS.gene068620:CDS
CTGCTCCTCTCCTTTGTGCT+GGG 0.434632 5.1:-21445968 MS.gene068620:CDS
TATCGATTTGTTATTGACAT+TGG 0.438397 5.1:-21445200 MS.gene068620:CDS
CGCCACAACTTTGACATGAA+TGG 0.444538 5.1:+21446141 None:intergenic
CATCCTCTAATGCCTTTGCT+TGG 0.446345 5.1:-21445551 MS.gene068620:CDS
AATATGTTATAGGCTGTAAT+GGG 0.447072 5.1:-21445611 MS.gene068620:intron
AGTCAGCTCCTAAGTGTTCA+AGG 0.448826 5.1:+21445781 None:intergenic
TGGAGCTCCAGAGAAGCCTT+TGG 0.454095 5.1:-21445495 MS.gene068620:CDS
TTAGAGTACCCAGGAACTAC+TGG 0.455836 5.1:+21446366 None:intergenic
AAAACTTCAAAATTGGAGAC+AGG 0.457788 5.1:-21446193 MS.gene068620:CDS
TGTTGAAGACATGAAGTTGC+TGG 0.463326 5.1:-21446245 MS.gene068620:intron
AATGGTAAACTTGTTATGGT+TGG 0.469139 5.1:-21445515 MS.gene068620:CDS
GGACTGCACATAGGTGTTGT+TGG 0.478905 5.1:-21445900 MS.gene068620:CDS
AAGCTTTCGGATGGGCAGCT+AGG 0.490753 5.1:-21447118 MS.gene068620:CDS
GCATCAAGTGATAAGGCATC+AGG 0.495160 5.1:+21445993 None:intergenic
AATTGGAGACAGGGTTGGTG+TGG 0.496167 5.1:-21446183 MS.gene068620:CDS
AGCTTTCGGATGGGCAGCTA+GGG 0.501441 5.1:-21447117 MS.gene068620:CDS
CAAGTCCAAAATGTTTCATA+GGG 0.502873 5.1:+21445931 None:intergenic
TGGGCAGCTAGGGACACATC+TGG 0.503109 5.1:-21447107 MS.gene068620:CDS
GAGTATGATTGGAGGGATGA+AGG 0.509991 5.1:-21445339 MS.gene068620:CDS
TTTCAGGGAAACAGGGGAGA+AGG 0.511637 5.1:-21446472 MS.gene068620:intron
GTTGATGGCACTATCACCTA+TGG 0.515552 5.1:-21446065 MS.gene068620:CDS
GGAAACAGGGGAGAAGGATG+TGG 0.515934 5.1:-21446466 MS.gene068620:CDS
AGCAGCGGCATCAAGTGATA+AGG 0.524931 5.1:+21445986 None:intergenic
TAGAGAACTTGAAAGGAGAA+AGG 0.526536 5.1:+21447081 None:intergenic
TTCAAAATTGGAGACAGGGT+TGG 0.531023 5.1:-21446188 MS.gene068620:CDS
TGCCATTCATGTCAAAGTTG+TGG 0.533789 5.1:-21446143 MS.gene068620:CDS
TTGGCAGGGAGAAAGACAAT+TGG 0.543249 5.1:-21445365 MS.gene068620:intron
ATTGGAGACAGGGTTGGTGT+GGG 0.544734 5.1:-21446182 MS.gene068620:CDS
GCAGGGAGAAAGACAATTGG+TGG 0.544871 5.1:-21445362 MS.gene068620:intron
TGACATTGGAAACACAATGA+AGG 0.546508 5.1:-21445186 MS.gene068620:CDS
TAGAGGATGGAGTGCTGAAA+CGG 0.546944 5.1:+21445565 None:intergenic
TGATATTGAGGTTGTTCCAG+TGG 0.550536 5.1:-21445270 MS.gene068620:CDS
GCAGTATTTACATAATCCAC+TGG 0.550557 5.1:+21445254 None:intergenic
CTTAGGAGCTGACTCATTCG+TGG 0.554856 5.1:-21445772 MS.gene068620:CDS
AAGGAAGCCCTTGAACACTT+AGG 0.559865 5.1:-21445789 MS.gene068620:CDS
ACAACACCTATGTGCAGTCC+AGG 0.560025 5.1:+21445903 None:intergenic
AGCACAAAGGAGAGGAGCAG+CGG 0.561956 5.1:+21445971 None:intergenic
CACAGTTACCTTAACCCCAA+AGG 0.563973 5.1:+21445836 None:intergenic
TGGCGTAAAACCTGATATTG+AGG 0.570134 5.1:-21445282 MS.gene068620:CDS
CAAGCAAAGGCATTAGAGGA+TGG 0.578766 5.1:+21445552 None:intergenic
GAAAATACTGGAAACTCCAA+AGG 0.580584 5.1:+21445479 None:intergenic
CTACTTACCCAGTAGTTCCT+GGG 0.589206 5.1:-21446374 MS.gene068620:intron
TTGGTGGGAGTATGATTGGA+GGG 0.589557 5.1:-21445346 MS.gene068620:CDS
ATTGGTGGGAGTATGATTGG+AGG 0.590145 5.1:-21445347 MS.gene068620:CDS
TCACATGGCTAAGAATGAGT+GGG 0.592665 5.1:-21446403 MS.gene068620:CDS
AGTGACAGAGGTGGGAAGCA+AGG 0.592868 5.1:-21446219 MS.gene068620:CDS
TACCTTCTAGAGAACTTGAA+AGG 0.593400 5.1:+21447074 None:intergenic
AAACTTCAAAATTGGAGACA+GGG 0.597248 5.1:-21446192 MS.gene068620:CDS
TTCACATGGCTAAGAATGAG+TGG 0.604893 5.1:-21446404 MS.gene068620:CDS
GGTGTCAGAGTAGCCTCCAT+AGG 0.609083 5.1:+21446049 None:intergenic
GGATTATGTAAATACTGCAA+TGG 0.611722 5.1:-21445249 MS.gene068620:CDS
GAAGTTCGCCAAAGCCTTTG+GGG 0.621609 5.1:-21445850 MS.gene068620:CDS
TAGAGTACCCAGGAACTACT+GGG 0.622352 5.1:+21446367 None:intergenic
TTCTTCAATAGACCAAGCAA+AGG 0.622643 5.1:+21445539 None:intergenic
GACAAACCTGGACTGCACAT+AGG 0.623406 5.1:-21445909 MS.gene068620:CDS
GTCAGCTCCTAAGTGTTCAA+GGG 0.623420 5.1:+21445782 None:intergenic
ACTCATTCTTAGCCATGTGA+AGG 0.631347 5.1:+21446406 None:intergenic
TCGCGAACAAGACCAGATGA+AGG 0.635463 5.1:-21445745 MS.gene068620:intron
TTGGAGACAGGGTTGGTGTG+GGG 0.642743 5.1:-21446181 MS.gene068620:CDS
GCTGGTGAAGTGACAGAGGT+GGG 0.643936 5.1:-21446227 MS.gene068620:CDS
ATGTCACTCGGACCTTCACA+TGG 0.644647 5.1:-21446418 MS.gene068620:CDS
CAGGGAGAAAGACAATTGGT+GGG 0.647955 5.1:-21445361 MS.gene068620:CDS
GTGATCCCAGCACAAAGGAG+AGG 0.651936 5.1:+21445963 None:intergenic
CTTTGGGGTTAAGGTAACTG+TGG 0.655334 5.1:-21445835 MS.gene068620:CDS
TGCTGGTGAAGTGACAGAGG+TGG 0.668343 5.1:-21446228 MS.gene068620:CDS
TTCATGTCAAAGTTGTGGCG+AGG 0.670954 5.1:-21446138 MS.gene068620:CDS
TGGAGGCTACTCTGACACCA+TGG 0.673114 5.1:-21446045 MS.gene068620:CDS
CACATGGCTAAGAATGAGTG+GGG 0.673842 5.1:-21446402 MS.gene068620:CDS
GATGGCACTATCACCTATGG+AGG 0.687126 5.1:-21446062 MS.gene068620:CDS
AAAGTGTTCGTCGACAACCA+TGG 0.696527 5.1:+21446028 None:intergenic
ACACTGTGATCCCAGCACAA+AGG 0.700831 5.1:+21445958 None:intergenic
AGTTGCTGGTGAAGTGACAG+AGG 0.727660 5.1:-21446231 MS.gene068620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTATAAAAAAAAAAATC+AGG + chr5.1:21445623-21445642 None:intergenic 10.0%
!! TTACTATATAGAAATAAATT+TGG + chr5.1:21446647-21446666 None:intergenic 10.0%
!! AATGTTATTCAATATGTTAT+AGG - chr5.1:21446706-21446725 MS.gene068620:intron 15.0%
!! GTTGAAAATTAAATAAACAA+TGG + chr5.1:21446001-21446020 None:intergenic 15.0%
!! TGTGTTATATTAAAATATGA+AGG - chr5.1:21446912-21446931 MS.gene068620:intron 15.0%
!! TTGAATAACATTAGAATTAA+AGG + chr5.1:21446698-21446717 None:intergenic 15.0%
!!! AAATTAGTTTGTTTTTATGA+CGG + chr5.1:21446039-21446058 None:intergenic 15.0%
!!! GACATATGATTTATTTTAAT+TGG - chr5.1:21445686-21445705 MS.gene068620:intron 15.0%
!! AAAACATCTCATAAATGATA+GGG - chr5.1:21445442-21445461 MS.gene068620:intron 20.0%
!! AAGTTAAAAGATTTAAAGAC+AGG - chr5.1:21445516-21445535 MS.gene068620:CDS 20.0%
!! AGTTAAAAGATTTAAAGACA+GGG - chr5.1:21445517-21445536 MS.gene068620:CDS 20.0%
!! TATTACATCACATTAATTGT+TGG - chr5.1:21446943-21446962 MS.gene068620:intron 20.0%
!! TTATAATAACAATTTCACCA+AGG + chr5.1:21445304-21445323 None:intergenic 20.0%
!!! AATATTTTTCATTCCTTTTC+AGG - chr5.1:21445839-21445858 MS.gene068620:CDS 20.0%
!!! ATATTTTTCATTCCTTTTCA+GGG - chr5.1:21445840-21445859 MS.gene068620:CDS 20.0%
! AAGGTGAAAAACTTCAAAAT+TGG - chr5.1:21446127-21446146 MS.gene068620:CDS 25.0%
! AATATGTTATAGGCTGTAAT+GGG - chr5.1:21446716-21446735 MS.gene068620:intron 25.0%
! ACTCATTAAAAGCGAATTAT+TGG + chr5.1:21446614-21446633 None:intergenic 25.0%
! CAACTTGATTGACATTAAAA+GGG + chr5.1:21445366-21445385 None:intergenic 25.0%
! GAAAACATCTCATAAATGAT+AGG - chr5.1:21445441-21445460 MS.gene068620:intron 25.0%
!! TATAGTAATCGATTTATCGA+TGG - chr5.1:21446659-21446678 MS.gene068620:intron 25.0%
!!! GAATAATGGTAAACTTGTTA+TGG - chr5.1:21446808-21446827 MS.gene068620:intron 25.0%
!!! TATCGATTTGTTATTGACAT+TGG - chr5.1:21447127-21447146 MS.gene068620:CDS 25.0%
AAAACTTCAAAATTGGAGAC+AGG - chr5.1:21446134-21446153 MS.gene068620:CDS 30.0%
AAACTTCAAAATTGGAGACA+GGG - chr5.1:21446135-21446154 MS.gene068620:CDS 30.0%
CAAGTCCAAAATGTTTCATA+GGG + chr5.1:21446399-21446418 None:intergenic 30.0%
CAATATGTTATAGGCTGTAA+TGG - chr5.1:21446715-21446734 MS.gene068620:intron 30.0%
CCAACTTGATTGACATTAAA+AGG + chr5.1:21445367-21445386 None:intergenic 30.0%
CTTGGTCTATTGAAGAATAA+TGG - chr5.1:21446794-21446813 MS.gene068620:intron 30.0%
GGATTATGTAAATACTGCAA+TGG - chr5.1:21447078-21447097 MS.gene068620:CDS 30.0%
TACTTCTCCTAACAAAAAGA+AGG - chr5.1:21446519-21446538 MS.gene068620:intron 30.0%
TCAAGTCCAAAATGTTTCAT+AGG + chr5.1:21446400-21446419 None:intergenic 30.0%
! CCAGTATTTTCTTTGATTGT+GGG - chr5.1:21446860-21446879 MS.gene068620:intron 30.0%
! CCTTTTAATGTCAATCAAGT+TGG - chr5.1:21445364-21445383 MS.gene068620:intron 30.0%
! TATAGCCCTATGAAACATTT+TGG - chr5.1:21446391-21446410 MS.gene068620:CDS 30.0%
! TCCAGTATTTTCTTTGATTG+TGG - chr5.1:21446859-21446878 MS.gene068620:intron 30.0%
!! AATGGTAAACTTGTTATGGT+TGG - chr5.1:21446812-21446831 MS.gene068620:intron 30.0%
!! GCATTCAAAGTACTATTTTG+CGG - chr5.1:21445883-21445902 MS.gene068620:CDS 30.0%
!!! ATTTTAATTGGCTCCTTGTA+TGG - chr5.1:21445698-21445717 MS.gene068620:intron 30.0%
!!! CAATCAAGTTGGTTTTCAAT+TGG - chr5.1:21445375-21445394 MS.gene068620:intron 30.0%
ACATCACATTAATTGTTGGC+AGG - chr5.1:21446947-21446966 MS.gene068620:intron 35.0%
ACTGGATCATCTCTAATGAA+CGG - chr5.1:21445465-21445484 MS.gene068620:intron 35.0%
ATCTCATAAATGATAGGGAC+TGG - chr5.1:21445447-21445466 MS.gene068620:intron 35.0%
CATCACATTAATTGTTGGCA+GGG - chr5.1:21446948-21446967 MS.gene068620:intron 35.0%
CCCACAATCAAAGAAAATAC+TGG + chr5.1:21446863-21446882 None:intergenic 35.0%
GAAAATACTGGAAACTCCAA+AGG + chr5.1:21446851-21446870 None:intergenic 35.0%
GCAGTATTTACATAATCCAC+TGG + chr5.1:21447076-21447095 None:intergenic 35.0%
TACAGTTCCAAGAATGTTGA+TGG - chr5.1:21446247-21446266 MS.gene068620:intron 35.0%
TACATCACACATCAACATTC+AGG + chr5.1:21445596-21445615 None:intergenic 35.0%
TAGAGAACTTGAAAGGAGAA+AGG + chr5.1:21445249-21445268 None:intergenic 35.0%
TGACATTGGAAACACAATGA+AGG - chr5.1:21447141-21447160 MS.gene068620:CDS 35.0%
TTCTTCAATAGACCAAGCAA+AGG + chr5.1:21446791-21446810 None:intergenic 35.0%
! ATTTTGCGGTATATGTCACT+CGG - chr5.1:21445897-21445916 MS.gene068620:CDS 35.0%
! TACCTTCTAGAGAACTTGAA+AGG + chr5.1:21445256-21445275 None:intergenic 35.0%
! TCTCTTATGTTTTCTCAAGC+TGG + chr5.1:21445553-21445572 None:intergenic 35.0%
!! ATTGATTTTGCAGCCAAACA+TGG - chr5.1:21447025-21447044 MS.gene068620:intron 35.0%
ACTCATTCTTAGCCATGTGA+AGG + chr5.1:21445924-21445943 None:intergenic 40.0%
ACTGGAACAACCTCAATATC+AGG + chr5.1:21447058-21447077 None:intergenic 40.0%
AGGATGCATGAATACATGAG+TGG + chr5.1:21445284-21445303 None:intergenic 40.0%
ATCCCAAAAAAGCTTTCGGA+TGG - chr5.1:21445200-21445219 MS.gene068620:CDS 40.0%
CTCATGTATTCATGCATCCT+TGG - chr5.1:21445284-21445303 MS.gene068620:CDS 40.0%
GAGCATCCCAAAAAAGCTTT+CGG - chr5.1:21445196-21445215 MS.gene068620:CDS 40.0%
GATAGTGCCATCAACATTCT+TGG + chr5.1:21446257-21446276 None:intergenic 40.0%
TCACATGGCTAAGAATGAGT+GGG - chr5.1:21445924-21445943 MS.gene068620:CDS 40.0%
TCCCAAAAAAGCTTTCGGAT+GGG - chr5.1:21445201-21445220 MS.gene068620:CDS 40.0%
TGATATTGAGGTTGTTCCAG+TGG - chr5.1:21447057-21447076 MS.gene068620:intron 40.0%
TGCCATTCATGTCAAAGTTG+TGG - chr5.1:21446184-21446203 MS.gene068620:CDS 40.0%
TGGCGTAAAACCTGATATTG+AGG - chr5.1:21447045-21447064 MS.gene068620:intron 40.0%
TGTTGAAGACATGAAGTTGC+TGG - chr5.1:21446082-21446101 MS.gene068620:CDS 40.0%
TTCAAAATTGGAGACAGGGT+TGG - chr5.1:21446139-21446158 MS.gene068620:CDS 40.0%
TTCACATGGCTAAGAATGAG+TGG - chr5.1:21445923-21445942 MS.gene068620:CDS 40.0%
TTGACATTAAAAGGGACGCA+AGG + chr5.1:21445358-21445377 None:intergenic 40.0%
! ACAATTGGTGGGAGTATGAT+TGG - chr5.1:21446977-21446996 MS.gene068620:intron 40.0%
! AGGGCTTCCTTCTTTTTGTT+AGG + chr5.1:21446529-21446548 None:intergenic 40.0%
! ATCAGGTTTTACGCCATGTT+TGG + chr5.1:21447041-21447060 None:intergenic 40.0%
! CATTTTGGACTTGACAAACC+TGG - chr5.1:21446406-21446425 MS.gene068620:CDS 40.0%
! GTGATAAGGCATCAGGAATA+TGG + chr5.1:21446330-21446349 None:intergenic 40.0%
! TTTGTGATGTTAGAGTACCC+AGG + chr5.1:21445973-21445992 None:intergenic 40.0%
!! ATTCCTTTTCAGGGAAACAG+GGG - chr5.1:21445849-21445868 MS.gene068620:CDS 40.0%
!! CATTCCTTTTCAGGGAAACA+GGG - chr5.1:21445848-21445867 MS.gene068620:CDS 40.0%
!! CTCCTTTCAAGTTCTCTAGA+AGG - chr5.1:21445251-21445270 MS.gene068620:CDS 40.0%
!! TCATTCCTTTTCAGGGAAAC+AGG - chr5.1:21445847-21445866 MS.gene068620:CDS 40.0%
!! TCTTGGTGGATTAGGTCATA+TGG - chr5.1:21446450-21446469 MS.gene068620:CDS 40.0%
!!! ATGCTCCATTTCTGGTTTTG+AGG + chr5.1:21445182-21445201 None:intergenic 40.0%
!!! TTTTTGGGATGCTCCATTTC+TGG + chr5.1:21445190-21445209 None:intergenic 40.0%
AAGGAAGCCCTTGAACACTT+AGG - chr5.1:21446538-21446557 MS.gene068620:intron 45.0%
ACTACTTACCCAGTAGTTCC+TGG - chr5.1:21445952-21445971 MS.gene068620:CDS 45.0%
AGTCAGCTCCTAAGTGTTCA+AGG + chr5.1:21446549-21446568 None:intergenic 45.0%
CACAGTTACCTTAACCCCAA+AGG + chr5.1:21446494-21446513 None:intergenic 45.0%
CACATGGCTAAGAATGAGTG+GGG - chr5.1:21445925-21445944 MS.gene068620:CDS 45.0%
CAGGGAGAAAGACAATTGGT+GGG - chr5.1:21446966-21446985 MS.gene068620:intron 45.0%
CATCCTCTAATGCCTTTGCT+TGG - chr5.1:21446776-21446795 MS.gene068620:intron 45.0%
CGCCACAACTTTGACATGAA+TGG + chr5.1:21446189-21446208 None:intergenic 45.0%
CTACTTACCCAGTAGTTCCT+GGG - chr5.1:21445953-21445972 MS.gene068620:CDS 45.0%
GAGTATGATTGGAGGGATGA+AGG - chr5.1:21446988-21447007 MS.gene068620:intron 45.0%
GTCAGCTCCTAAGTGTTCAA+GGG + chr5.1:21446548-21446567 None:intergenic 45.0%
TACCTTAACCCCAAAGGCTT+TGG + chr5.1:21446488-21446507 None:intergenic 45.0%
TATTGGTACGCACCTTCATC+TGG + chr5.1:21446597-21446616 None:intergenic 45.0%
TGAAGTTCGCCAAAGCCTTT+GGG - chr5.1:21446476-21446495 MS.gene068620:intron 45.0%
TGGCTCCTTGTATGGTAAGA+GGG - chr5.1:21445706-21445725 MS.gene068620:intron 45.0%
TTCATGTCAAAGTTGTGGCG+AGG - chr5.1:21446189-21446208 MS.gene068620:CDS 45.0%
TTGGCAGGGAGAAAGACAAT+TGG - chr5.1:21446962-21446981 MS.gene068620:intron 45.0%
TTGGCTCCTTGTATGGTAAG+AGG - chr5.1:21445705-21445724 MS.gene068620:intron 45.0%
! AAAGTGTTCGTCGACAACCA+TGG + chr5.1:21446302-21446321 None:intergenic 45.0%
! ATTGGTGGGAGTATGATTGG+AGG - chr5.1:21446980-21446999 MS.gene068620:intron 45.0%
! GCATCAAGTGATAAGGCATC+AGG + chr5.1:21446337-21446356 None:intergenic 45.0%
! TAGAGTACCCAGGAACTACT+GGG + chr5.1:21445963-21445982 None:intergenic 45.0%
! TTAGAGTACCCAGGAACTAC+TGG + chr5.1:21445964-21445983 None:intergenic 45.0%
! TTGGTGGGAGTATGATTGGA+GGG - chr5.1:21446981-21447000 MS.gene068620:intron 45.0%
!! AGACCAAGCAAAGGCATTAG+AGG + chr5.1:21446782-21446801 None:intergenic 45.0%
!! ATAGGTGTTGTTGGTCTTGG+TGG - chr5.1:21446436-21446455 MS.gene068620:CDS 45.0%
!! CAAGCAAAGGCATTAGAGGA+TGG + chr5.1:21446778-21446797 None:intergenic 45.0%
!! CACATAGGTGTTGTTGGTCT+TGG - chr5.1:21446433-21446452 MS.gene068620:CDS 45.0%
!! CGGAGAGTGCTGTGAAATTT+AGG - chr5.1:21445485-21445504 MS.gene068620:CDS 45.0%
!! CTTTGGGGTTAAGGTAACTG+TGG - chr5.1:21446492-21446511 MS.gene068620:intron 45.0%
!! GTTGATGGCACTATCACCTA+TGG - chr5.1:21446262-21446281 MS.gene068620:intron 45.0%
!! GTTGTTGGTCTTGGTGGATT+AGG - chr5.1:21446442-21446461 MS.gene068620:CDS 45.0%
!! TAGAGGATGGAGTGCTGAAA+CGG + chr5.1:21446765-21446784 None:intergenic 45.0%
!!! GCCCATCCGAAAGCTTTTTT+GGG + chr5.1:21445205-21445224 None:intergenic 45.0%
!!! TGCCCATCCGAAAGCTTTTT+TGG + chr5.1:21445206-21445225 None:intergenic 45.0%
ACAACACCTATGTGCAGTCC+AGG + chr5.1:21446427-21446446 None:intergenic 50.0%
ACACTGTGATCCCAGCACAA+AGG + chr5.1:21446372-21446391 None:intergenic 50.0%
AGCAGCGGCATCAAGTGATA+AGG + chr5.1:21446344-21446363 None:intergenic 50.0%
AGTTGCTGGTGAAGTGACAG+AGG - chr5.1:21446096-21446115 MS.gene068620:CDS 50.0%
ATGTCACTCGGACCTTCACA+TGG - chr5.1:21445909-21445928 MS.gene068620:CDS 50.0%
CAGCACCCTCTTACCATACA+AGG + chr5.1:21445714-21445733 None:intergenic 50.0%
GAAGTTCGCCAAAGCCTTTG+GGG - chr5.1:21446477-21446496 MS.gene068620:intron 50.0%
GACAAACCTGGACTGCACAT+AGG - chr5.1:21446418-21446437 MS.gene068620:CDS 50.0%
GATGGCACTATCACCTATGG+AGG - chr5.1:21446265-21446284 MS.gene068620:intron 50.0%
GCACATCACTCGCGAGAAAT+AGG - chr5.1:21445783-21445802 MS.gene068620:CDS 50.0%
GCAGGGAGAAAGACAATTGG+TGG - chr5.1:21446965-21446984 MS.gene068620:intron 50.0%
GGAAACTCCAAAGGCTTCTC+TGG + chr5.1:21446842-21446861 None:intergenic 50.0%
GTGAAGTTCGCCAAAGCCTT+TGG - chr5.1:21446475-21446494 MS.gene068620:intron 50.0%
TCGCGAACAAGACCAGATGA+AGG - chr5.1:21446582-21446601 MS.gene068620:intron 50.0%
TCTCCCCTGTTTCCCTGAAA+AGG + chr5.1:21445855-21445874 None:intergenic 50.0%
TTTCAGGGAAACAGGGGAGA+AGG - chr5.1:21445855-21445874 MS.gene068620:CDS 50.0%
! CTTAGGAGCTGACTCATTCG+TGG - chr5.1:21446555-21446574 MS.gene068620:intron 50.0%
! GGACTGCACATAGGTGTTGT+TGG - chr5.1:21446427-21446446 MS.gene068620:CDS 50.0%
!! AATTGGAGACAGGGTTGGTG+TGG - chr5.1:21446144-21446163 MS.gene068620:CDS 50.0%
!! ATTGGAGACAGGGTTGGTGT+GGG - chr5.1:21446145-21446164 MS.gene068620:CDS 50.0%
!! GCTGTAATGGGCACGTTTGA+TGG - chr5.1:21446728-21446747 MS.gene068620:intron 50.0%
!! GTGCTGACTAGTGGTAATGC+AGG - chr5.1:21445728-21445747 MS.gene068620:intron 50.0%
AAGCTTTCGGATGGGCAGCT+AGG - chr5.1:21445209-21445228 MS.gene068620:CDS 55.0%
AGCACAAAGGAGAGGAGCAG+CGG + chr5.1:21446359-21446378 None:intergenic 55.0%
AGCTTTCGGATGGGCAGCTA+GGG - chr5.1:21445210-21445229 MS.gene068620:CDS 55.0%
AGTGACAGAGGTGGGAAGCA+AGG - chr5.1:21446108-21446127 MS.gene068620:CDS 55.0%
CTGCTCCTCTCCTTTGTGCT+GGG - chr5.1:21446359-21446378 MS.gene068620:intron 55.0%
GCTGGTGAAGTGACAGAGGT+GGG - chr5.1:21446100-21446119 MS.gene068620:CDS 55.0%
GGAAACAGGGGAGAAGGATG+TGG - chr5.1:21445861-21445880 MS.gene068620:CDS 55.0%
GGTGTCAGAGTAGCCTCCAT+AGG + chr5.1:21446281-21446300 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG + chr5.1:21446367-21446386 None:intergenic 55.0%
TGCTGGTGAAGTGACAGAGG+TGG - chr5.1:21446099-21446118 MS.gene068620:CDS 55.0%
TGGAGCTCCAGAGAAGCCTT+TGG - chr5.1:21446832-21446851 MS.gene068620:intron 55.0%
TGGAGGCTACTCTGACACCA+TGG - chr5.1:21446282-21446301 MS.gene068620:intron 55.0%
!! CGCCAAAGCCTTTGGGGTTA+AGG - chr5.1:21446483-21446502 MS.gene068620:intron 55.0%
!! GGTAAGAGGGTGCTGACTAG+TGG - chr5.1:21445719-21445738 MS.gene068620:intron 55.0%
!! TTGGAGACAGGGTTGGTGTG+GGG - chr5.1:21446146-21446165 MS.gene068620:CDS 55.0%
GCTGCTCCTCTCCTTTGTGC+TGG - chr5.1:21446358-21446377 MS.gene068620:intron 60.0%
GTTGGTGTGGGGTGCATGGT+TGG - chr5.1:21446157-21446176 MS.gene068620:CDS 60.0%
TGGGCAGCTAGGGACACATC+TGG - chr5.1:21445220-21445239 MS.gene068620:CDS 60.0%
!! CAGGGTTGGTGTGGGGTGCA+TGG - chr5.1:21446153-21446172 MS.gene068620:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 21445175 21447174 21445175 ID=MS.gene068620
chr5.1 mRNA 21445175 21447174 21445175 ID=MS.gene068620.t1;Parent=MS.gene068620
chr5.1 exon 21447077 21447174 21447077 ID=MS.gene068620.t1.exon1;Parent=MS.gene068620.t1
chr5.1 CDS 21447077 21447174 21447077 ID=cds.MS.gene068620.t1;Parent=MS.gene068620.t1
chr5.1 exon 21446375 21446488 21446375 ID=MS.gene068620.t1.exon2;Parent=MS.gene068620.t1
chr5.1 CDS 21446375 21446488 21446375 ID=cds.MS.gene068620.t1;Parent=MS.gene068620.t1
chr5.1 exon 21445746 21446259 21445746 ID=MS.gene068620.t1.exon3;Parent=MS.gene068620.t1
chr5.1 CDS 21445746 21446259 21445746 ID=cds.MS.gene068620.t1;Parent=MS.gene068620.t1
chr5.1 exon 21445468 21445621 21445468 ID=MS.gene068620.t1.exon4;Parent=MS.gene068620.t1
chr5.1 CDS 21445468 21445621 21445468 ID=cds.MS.gene068620.t1;Parent=MS.gene068620.t1
chr5.1 exon 21445175 21445380 21445175 ID=MS.gene068620.t1.exon5;Parent=MS.gene068620.t1
chr5.1 CDS 21445175 21445380 21445175 ID=cds.MS.gene068620.t1;Parent=MS.gene068620.t1
Gene Sequence

>MS.gene068620

ATGGCCTCAAAACCAGAAATGGAGCATCCCAAAAAAGCTTTCGGATGGGCAGCTAGGGACACATCTGGAGTCCTTTCTCCTTTCAAGTTCTCTAGAAGGTATTTAACCACTCATGTATTCATGCATCCTTGGTGAAATTGTTATTATAACAACAATAAAAGCTACATTTTATTACATTATCCTTGCGTCCCTTTTAATGTCAATCAAGTTGGTTTTCAATTGGTATAATGATTTATGTTGCTTGATTGTTGAGTGATAAAATCAAAGAAAACATCTCATAAATGATAGGGACTGGATCATCTCTAATGAACGGAGAGTGCTGTGAAATTTAGGTCATAAAAAAGTTAAAAGATTTAAAGACAGGGTGTGAATTGTCCAGCTTGAGAAAACATAAGAGATTTTCATTGTAGAGATTCATCCTGAATGTTGATGTGTGATGTAAAATCCTGATTTTTTTTTTTATAGATAATGCTAAAGATCGAGAAGCATTGTTATAGCGAAAATCACGATTGACATATGATTTATTTTAATTGGCTCCTTGTATGGTAAGAGGGTGCTGACTAGTGGTAATGCAGGTGCTATTTGTGTCGTAATGCTAATTAACACGAGCACATCACTCGCGAGAAATAGGACTGCTCTTTTTTATATATATATTTAAATCGATAATATTTTTCATTCCTTTTCAGGGAAACAGGGGAGAAGGATGTGGCATTCAAAGTACTATTTTGCGGTATATGTCACTCGGACCTTCACATGGCTAAGAATGAGTGGGGCAGTACTACTTACCCAGTAGTTCCTGGGTACTCTAACATCACAAAACAAACCATTGTTTATTTAATTTTCAACGAATTAAACATTACTCCGTCATAAAAACAAACTAATTTTTTTGTTTCTTTCTCATCTCATTTGTTGAAGACATGAAGTTGCTGGTGAAGTGACAGAGGTGGGAAGCAAGGTGAAAAACTTCAAAATTGGAGACAGGGTTGGTGTGGGGTGCATGGTTGGATCTTGCCATTCATGTCAAAGTTGTGGCGAGGATCTCGAAAATTACTGCTCAAAGATGATTCAGACATACAGTTCCAAGAATGTTGATGGCACTATCACCTATGGAGGCTACTCTGACACCATGGTTGTCGACGAACACTTTGTTGTCCATATTCCTGATGCCTTATCACTTGATGCCGCTGCTCCTCTCCTTTGTGCTGGGATCACAGTGTATAGCCCTATGAAACATTTTGGACTTGACAAACCTGGACTGCACATAGGTGTTGTTGGTCTTGGTGGATTAGGTCATATGGCTGTGAAGTTCGCCAAAGCCTTTGGGGTTAAGGTAACTGTGGTAAGTACTTCTCCTAACAAAAAGAAGGAAGCCCTTGAACACTTAGGAGCTGACTCATTCGTGGTTAGTCGCGAACAAGACCAGATGAAGGTGCGTACCAATAATTCGCTTTTAATGAGTAATTTAGAAACCAAATTTATTTCTATATAGTAATCGATTTATCGATGGTCTGAAAAACTGACCTTTAATTCTAATGTTATTCAATATGTTATAGGCTGTAATGGGCACGTTTGATGGAATCATCGACACCGTTTCAGCACTCCATCCTCTAATGCCTTTGCTTGGTCTATTGAAGAATAATGGTAAACTTGTTATGGTTGGAGCTCCAGAGAAGCCTTTGGAGTTTCCAGTATTTTCTTTGATTGTGGGTAAGCATATGATAAAAATTCTATACAGACTGTGTTATATTAAAATATGAAGGTTGTTAAATATTACATCACATTAATTGTTGGCAGGGAGAAAGACAATTGGTGGGAGTATGATTGGAGGGATGAAGGAAACACAAGAAATGATTGATTTTGCAGCCAAACATGGCGTAAAACCTGATATTGAGGTTGTTCCAGTGGATTATGTAAATACTGCAATGGAGCGCTTACTCAAAGCTGATGTGAAATATCGATTTGTTATTGACATTGGAAACACAATGAAGGCAAGCTCTTAA
Protein sequence

>MS.gene068620.t1

MASKPEMEHPKKAFGWAARDTSGVLSPFKFSRRETGEKDVAFKVLFCGICHSDLHMAKNEWGSTTYPVVPGHEVAGEVTEVGSKVKNFKIGDRVGVGCMVGSCHSCQSCGEDLENYCSKMIQTYSSKNVDGTITYGGYSDTMVVDEHFVVHIPDALSLDAAAPLLCAGITVYSPMKHFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGVKVTVVSTSPNKKKEALEHLGADSFVVSREQDQMKAVMGTFDGIIDTVSALHPLMPLLGLLKNNGKLVMVGAPEKPLEFPVFSLIVGRKTIGGSMIGGMKETQEMIDFAAKHGVKPDIEVVPVDYVNTAMERLLKADVKYRFVIDIGNTMKASS