AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027824

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027824.t1 MTR_5g031210 96.399 361 13 0 1 361 1 361 0.0 719
MS.gene027824.t1 MTR_5g031230 93.906 361 22 0 1 361 1 361 0.0 673
MS.gene027824.t1 MTR_4g115300 83.934 361 58 0 1 361 1 361 0.0 623
MS.gene027824.t1 MTR_5g031250 83.102 361 61 0 1 361 1 361 0.0 610
MS.gene027824.t1 MTR_5g031305 80.609 361 69 1 1 360 1 361 0.0 587
MS.gene027824.t1 MTR_5g031310 80.055 361 71 1 1 360 1 361 0.0 585
MS.gene027824.t1 MTR_5g031390 79.224 361 75 0 1 361 1 361 0.0 583
MS.gene027824.t1 MTR_5g031300 78.453 362 77 1 1 361 1 362 0.0 578
MS.gene027824.t1 MTR_5g031360 76.068 351 84 0 10 360 12 362 0.0 553
MS.gene027824.t1 MTR_5g031190 73.407 361 96 0 1 361 1 361 0.0 548
MS.gene027824.t1 MTR_8g068190 75.833 360 87 0 1 360 1 360 0.0 540
MS.gene027824.t1 MTR_5g031180 76.454 361 85 0 1 361 1 361 0.0 539
MS.gene027824.t1 MTR_8g092630 62.429 354 132 1 4 357 61 413 1.83e-149 428
MS.gene027824.t1 MTR_5g031250 83.817 241 39 0 1 241 1 241 2.53e-143 406
MS.gene027824.t1 MTR_5g031430 59.420 345 140 0 14 358 14 358 3.03e-139 400
MS.gene027824.t1 MTR_1g025950 59.770 348 139 1 9 355 8 355 8.22e-137 394
MS.gene027824.t1 MTR_2g009810 54.046 346 157 2 14 358 62 406 5.18e-117 345
MS.gene027824.t1 MTR_1g107425 52.507 339 161 0 15 353 13 351 1.55e-116 342
MS.gene027824.t1 MTR_1g107425 52.688 279 131 1 15 293 13 290 1.35e-91 276
MS.gene027824.t1 MTR_2g009820 51.489 235 105 2 130 358 23 254 5.36e-65 207
MS.gene027824.t1 MTR_5g031290 74.561 114 29 0 247 360 80 193 3.40e-57 185
MS.gene027824.t1 MTR_5g031290 85.000 80 11 1 1 79 1 80 3.96e-43 148
MS.gene027824.t1 MTR_0001s0660 27.723 303 193 9 38 318 35 333 5.12e-20 91.3
MS.gene027824.t1 MTR_8g095220 27.000 300 178 10 7 279 59 344 5.37e-20 90.9
MS.gene027824.t1 MTR_8g095220 28.352 261 151 9 7 245 59 305 1.94e-19 88.2
MS.gene027824.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 4.09e-16 79.3
MS.gene027824.t1 MTR_3g089977 23.782 349 227 11 39 354 37 379 4.37e-16 79.3
MS.gene027824.t1 MTR_3g089940 24.189 339 219 10 39 344 37 370 5.31e-16 79.0
MS.gene027824.t1 MTR_6g466320 25.882 340 223 11 1 325 6 331 5.85e-16 78.6
MS.gene027824.t1 MTR_6g465010 25.882 340 223 11 1 325 6 331 5.85e-16 78.6
MS.gene027824.t1 MTR_3g089970 23.894 339 220 10 39 344 38 371 7.91e-16 78.6
MS.gene027824.t1 MTR_6g465010 26.266 316 205 10 25 325 13 315 3.39e-15 76.3
MS.gene027824.t1 MTR_5g045710 26.794 209 138 3 38 233 35 241 2.47e-14 73.9
MS.gene027824.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 4.76e-14 72.8
MS.gene027824.t1 MTR_3g089940 24.430 307 202 8 39 319 37 339 5.17e-14 72.8
MS.gene027824.t1 MTR_5g031380 62.500 40 15 0 81 120 20 59 1.53e-11 61.2
MS.gene027824.t1 MTR_7g068320 26.705 352 215 15 39 353 48 393 1.91e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027824.t1 AT4G37980 69.143 350 108 0 11 360 7 356 4.36e-172 483
MS.gene027824.t1 AT4G37990 68.286 350 111 0 11 360 7 356 1.99e-165 466
MS.gene027824.t1 AT4G39330 61.281 359 139 0 1 359 1 359 6.89e-164 462
MS.gene027824.t1 AT4G37970 62.254 355 134 0 6 360 7 361 1.06e-162 459
MS.gene027824.t1 AT2G21890 60.511 352 138 1 11 361 5 356 7.60e-154 437
MS.gene027824.t1 AT2G21730 60.623 353 137 2 11 361 5 357 9.19e-153 435
MS.gene027824.t1 AT4G37980 69.338 287 88 0 11 297 7 293 1.15e-134 386
MS.gene027824.t1 AT4G39330 61.462 301 116 0 1 301 1 301 6.38e-133 382
MS.gene027824.t1 AT4G37970 61.644 292 112 0 6 297 7 298 1.75e-126 365
MS.gene027824.t1 AT1G72680 51.143 350 166 2 11 358 9 355 4.77e-116 341
MS.gene027824.t1 AT3G19450 51.445 346 168 0 11 356 9 354 6.09e-108 320
MS.gene027824.t1 AT4G34230 51.873 347 167 0 7 353 4 350 2.25e-102 306
MS.gene027824.t1 AT4G34230 51.585 347 168 0 7 353 4 350 1.95e-101 304
MS.gene027824.t1 AT4G37970 61.603 237 91 0 6 242 7 243 1.99e-99 295
MS.gene027824.t1 AT4G37970 61.603 237 91 0 6 242 7 243 2.91e-99 295
MS.gene027824.t1 AT5G63620 25.076 331 205 11 7 308 61 377 1.54e-20 92.8
MS.gene027824.t1 AT5G63620 25.333 300 183 10 7 279 61 346 2.07e-20 92.4
MS.gene027824.t1 AT5G63620 26.744 258 153 9 7 242 61 304 5.29e-20 89.7
MS.gene027824.t1 AT1G32780 27.123 365 213 16 39 358 37 393 2.29e-16 80.1
MS.gene027824.t1 AT5G43940 27.143 350 210 12 39 352 36 376 2.88e-15 76.6
MS.gene027824.t1 AT5G43940 27.143 350 210 12 39 352 48 388 3.16e-15 76.6
MS.gene027824.t1 AT1G77120 24.138 348 233 10 36 354 33 378 5.46e-15 75.9
MS.gene027824.t1 AT1G22430 25.503 298 189 9 39 307 42 335 2.33e-14 73.9
MS.gene027824.t1 AT1G22430 25.503 298 189 9 39 307 42 335 2.33e-14 73.9
MS.gene027824.t1 AT1G22430 25.943 212 139 4 39 233 42 252 2.66e-13 70.5
MS.gene027824.t1 AT1G22430 25.943 212 139 4 39 233 42 252 3.94e-13 69.7
MS.gene027824.t1 AT1G22430 25.943 212 139 4 39 233 42 252 3.94e-13 69.7
MS.gene027824.t1 AT1G22440 26.738 187 119 4 39 208 40 225 1.16e-12 68.9
MS.gene027824.t1 AT1G64710 25.333 300 194 9 39 310 13 310 2.71e-12 67.8
MS.gene027824.t1 AT1G64710 25.333 300 194 9 39 310 36 333 2.75e-12 67.8
MS.gene027824.t1 AT4G22110 25.581 215 140 5 39 235 43 255 3.68e-11 63.5

Find 93 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTCTTTCCTTTAATTT+TGG 0.091002 5.2:+21539229 MS.gene027824:CDS
TTGTTTGGATGTTCAAATTC+AGG 0.242919 5.2:-21538066 None:intergenic
GCTAGCTTACCCAAAATTAA+AGG 0.255595 5.2:-21539239 None:intergenic
GTGAAATTTGCCAAAGCTTT+TGG 0.281096 5.2:+21538876 MS.gene027824:CDS
ATCAACCTATCCACTAGTTC+CGG 0.329909 5.2:+21538376 MS.gene027824:CDS
CATCCTATCTTGCCACTACT+TGG 0.340131 5.2:+21539146 MS.gene027824:CDS
GCCCAACCAAAGGCCTTGTT+TGG 0.342284 5.2:-21538081 None:intergenic
GCTGCACCTCTCCTTTGTGC+TGG 0.348614 5.2:+21538759 MS.gene027824:CDS
GTTGTTGGTCTTGGTGGGCT+TGG 0.363937 5.2:+21538843 MS.gene027824:CDS
CTGGTAGCTGGAAGTGGAAT+TGG 0.364565 5.2:+21539356 MS.gene027824:CDS
GACACGAAAATTTAAGAACT+TGG 0.385264 5.2:-21539542 None:intergenic
GATATTTGACATCTGCTTTG+AGG 0.389547 5.2:-21539487 None:intergenic
CATATCGGTGTTGTTGGTCT+TGG 0.391909 5.2:+21538834 MS.gene027824:CDS
GCTAGCAGATGTACTAATTA+CGG 0.396987 5.2:-21538910 None:intergenic
CACATTAGCACCAAAAGCTT+TGG 0.409643 5.2:-21538886 None:intergenic
TCTTGGTGGGCTTGGTCACA+TGG 0.411120 5.2:+21538851 MS.gene027824:CDS
TACAGTCCTCTTAGATACTT+TGG 0.411424 5.2:+21538792 MS.gene027824:CDS
GAACATCCAAACAAGGCCTT+TGG 0.412216 5.2:+21538075 MS.gene027824:CDS
ATCGGTGTTGTTGGTCTTGG+TGG 0.419928 5.2:+21538837 MS.gene027824:CDS
CTGCACCTCTCCTTTGTGCT+GGG 0.424848 5.2:+21538760 MS.gene027824:CDS
CGGAGGTTATTCTGACTCAT+TGG 0.425148 5.2:+21538683 MS.gene027824:CDS
ATAGTCTGCACTAGGTACTT+TGG 0.427527 5.2:+21539094 MS.gene027824:intron
TCAACCTATCCACTAGTTCC+GGG 0.430778 5.2:+21538377 MS.gene027824:CDS
TCCAAACAAGGCCTTTGGTT+GGG 0.431952 5.2:+21538080 MS.gene027824:CDS
GGTCTTCATATCGGTGTTGT+TGG 0.432442 5.2:+21538828 MS.gene027824:CDS
CCCAAAATTAAAGGAAAGAC+GGG 0.434247 5.2:-21539230 None:intergenic
CCCCTTTCAACTTCTCAAGA+AGG 0.434257 5.2:+21538130 MS.gene027824:CDS
AATCCAAGTAGTGGCAAGAT+AGG 0.440808 5.2:-21539149 None:intergenic
GACAAACCTGGTCTTCATAT+CGG 0.456603 5.2:+21538819 MS.gene027824:CDS
GCAGGGAGAAAGCTGGTAGC+TGG 0.462192 5.2:+21539344 MS.gene027824:intron
GGGATCTCCAGAGGCTTGTC+AGG 0.464857 5.2:-21539209 None:intergenic
GCTTCAAGTGGTAAGCCATC+AGG 0.468059 5.2:-21538735 None:intergenic
GATAGGATGATTTGCTGAAA+CGG 0.468221 5.2:-21539132 None:intergenic
TTATTCTGACTCATTGGTTG+CGG 0.471466 5.2:+21538689 MS.gene027824:CDS
AAATACAGGCATGAGATTGT+TGG 0.474794 5.2:+21538483 MS.gene027824:intron
AACCTTCTTGAGAAGTTGAA+AGG 0.479627 5.2:-21538132 None:intergenic
ACCTTCTTGAGAAGTTGAAA+GGG 0.480830 5.2:-21538131 None:intergenic
TACATCTGCTAGCAAAGAAA+AGG 0.482166 5.2:+21538920 MS.gene027824:CDS
TATCGATTTGTGCTCGACAT+TGG 0.486206 5.2:+21539506 MS.gene027824:CDS
CCCACATACACTACTACAAT+CGG 0.493624 5.2:+21538630 MS.gene027824:CDS
AGAAAGCTGGTAGCTGGAAG+TGG 0.497943 5.2:+21539350 MS.gene027824:CDS
TGGTGTACCTGACAAGCCTC+TGG 0.501156 5.2:+21539202 MS.gene027824:CDS
AAAGTAGAAAAGTTCAATGT+TGG 0.511069 5.2:+21538528 MS.gene027824:CDS
CTGCATGGCGAAGAACGAAT+GGG 0.515871 5.2:+21538349 MS.gene027824:CDS
TCGAGTCCAAAGTATCTAAG+AGG 0.516519 5.2:-21538798 None:intergenic
ATCCAAACAAGGCCTTTGGT+TGG 0.532747 5.2:+21538079 MS.gene027824:CDS
TCTGCATGGCGAAGAACGAA+TGG 0.536596 5.2:+21538348 MS.gene027824:CDS
TCGGTGTTGTTGGTCTTGGT+GGG 0.546049 5.2:+21538838 MS.gene027824:CDS
GTTGGTATAGTGACAAAGGT+GGG 0.549964 5.2:+21538501 MS.gene027824:CDS
TGGGCAGCTAGAGACACATC+TGG 0.550336 5.2:+21538099 MS.gene027824:CDS
ACAGGAACGACTTCAATGTC+AGG 0.550360 5.2:-21539434 None:intergenic
CTTGGATTACTGAAGTCTCA+TGG 0.554281 5.2:+21539164 MS.gene027824:CDS
ACCGATTGTAGTAGTGTATG+TGG 0.555469 5.2:-21538631 None:intergenic
ATCGGTGCCAAGCATCGTGA+TGG 0.559094 5.2:+21538648 MS.gene027824:CDS
CCGATTGTAGTAGTGTATGT+GGG 0.567124 5.2:-21538630 None:intergenic
ACCCAAAATTAAAGGAAAGA+CGG 0.568562 5.2:-21539231 None:intergenic
TACGTGGTTCACATTCCTGA+TGG 0.574374 5.2:+21538720 MS.gene027824:CDS
GTCTCTAGCTGCCCAACCAA+AGG 0.577379 5.2:-21538091 None:intergenic
TACTTTGGACTCGACAAACC+TGG 0.577584 5.2:+21538807 MS.gene027824:CDS
GTTGGAGACAAAGTAGGTGT+TGG 0.583429 5.2:+21538546 MS.gene027824:CDS
CATGGAAAACTTGTAATGGT+TGG 0.586324 5.2:+21539182 MS.gene027824:CDS
AGAGCATACCCCGGAACTAG+TGG 0.586460 5.2:-21538386 None:intergenic
GATGGTTCCATCACGATGCT+TGG 0.587249 5.2:-21538655 None:intergenic
TTCAATGTTGGAGACAAAGT+AGG 0.588338 5.2:+21538540 MS.gene027824:CDS
GTCTCATGGAAAACTTGTAA+TGG 0.588488 5.2:+21539178 MS.gene027824:CDS
AAGGAAGCAAAAGAACACTT+AGG 0.590978 5.2:+21538939 MS.gene027824:CDS
ATTATCGGCAGGGAGAAAGC+TGG 0.593426 5.2:+21539337 MS.gene027824:intron
AAGTGGAATTGGAAGCATCA+AGG 0.601852 5.2:+21539367 MS.gene027824:CDS
CGTGATGGAACCATCACATA+CGG 0.603134 5.2:+21538663 MS.gene027824:CDS
GACTTCAGTAATCCAAGTAG+TGG 0.605130 5.2:-21539158 None:intergenic
TCGTGACCAAGATAAAATGC+AGG 0.605547 5.2:+21538983 MS.gene027824:CDS
AGAATAACCTCCGTATGTGA+TGG 0.606221 5.2:-21538673 None:intergenic
TGTTGGTATAGTGACAAAGG+TGG 0.606925 5.2:+21538500 MS.gene027824:CDS
TGACATTGAAGTCGTTCCTG+TGG 0.621293 5.2:+21539436 MS.gene027824:CDS
CCTTCTTGAGAAGTTGAAAG+GGG 0.624053 5.2:-21538130 None:intergenic
GTGATCCCAGCACAAAGGAG+AGG 0.625373 5.2:-21538765 None:intergenic
GCATTCAAAATATTGTATTG+TGG 0.625490 5.2:+21538308 MS.gene027824:CDS
GCTGTGTTCACATAATCCAC+AGG 0.628030 5.2:-21539452 None:intergenic
ATACCCCGGAACTAGTGGAT+AGG 0.629537 5.2:-21538381 None:intergenic
TCTGCACTAGGTACTTTGGA+TGG 0.633602 5.2:+21539098 MS.gene027824:CDS
CGATTGTAGTAGTGTATGTG+GGG 0.641576 5.2:-21538629 None:intergenic
AGAGGTGCAGCAGCTTCAAG+TGG 0.642094 5.2:-21538747 None:intergenic
ATGTCACTCAGATGTCTGCA+TGG 0.642700 5.2:+21538334 MS.gene027824:CDS
GGAAAGACGGGGATCTCCAG+AGG 0.652974 5.2:-21539218 None:intergenic
TGCATGGCGAAGAACGAATG+GGG 0.656200 5.2:+21538350 MS.gene027824:CDS
GATTGTTGGTATAGTGACAA+AGG 0.656994 5.2:+21538497 MS.gene027824:CDS
CAACCTATCCACTAGTTCCG+GGG 0.659512 5.2:+21538378 MS.gene027824:CDS
ACAACACCGATATGAAGACC+AGG 0.677179 5.2:-21538825 None:intergenic
CCAAAATTAAAGGAAAGACG+GGG 0.687666 5.2:-21539229 None:intergenic
TGAATTTGAACATCCAAACA+AGG 0.697834 5.2:+21538068 MS.gene027824:CDS
ACACAGTGATCCCAGCACAA+AGG 0.705727 5.2:-21538770 None:intergenic
GATGGAACCATCACATACGG+AGG 0.744602 5.2:+21538666 MS.gene027824:CDS
GGTTGCGGATGAGAACTACG+TGG 0.814100 5.2:+21538704 MS.gene027824:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATATAAAAAATAC+AGG + chr5.2:21538469-21538488 MS.gene027824:intron 10.0%
!! AATTAGTTCATAAATTGATT+GGG - chr5.2:21539060-21539079 None:intergenic 15.0%
!! TAATTAGTTCATAAATTGAT+TGG - chr5.2:21539061-21539080 None:intergenic 15.0%
!! TAGTTCAATATTCTATTATA+CGG + chr5.2:21539017-21539036 MS.gene027824:intron 15.0%
!! ACTTATATGCTAACAATTAT+CGG + chr5.2:21539322-21539341 MS.gene027824:intron 20.0%
!! CAAATCAAAATAGTAGAAAA+TGG - chr5.2:21538446-21538465 None:intergenic 20.0%
!! TAATTGAAAAAAAAGTGAAG+TGG - chr5.2:21538168-21538187 None:intergenic 20.0%
!! TTAATCATATAAGTGATCTA+TGG - chr5.2:21539284-21539303 None:intergenic 20.0%
!!! TTAAGAGTAAAATTGTTGTT+AGG + chr5.2:21538224-21538243 MS.gene027824:intron 20.0%
! AAAGTAGAAAAGTTCAATGT+TGG + chr5.2:21538528-21538547 MS.gene027824:CDS 25.0%
! GCATTCAAAATATTGTATTG+TGG + chr5.2:21538308-21538327 MS.gene027824:CDS 25.0%
!! TTTATACCTGCATTTTATCT+TGG - chr5.2:21538992-21539011 None:intergenic 25.0%
AATTTAATTAGAGCATACCC+CGG - chr5.2:21538398-21538417 None:intergenic 30.0%
ACCCAAAATTAAAGGAAAGA+CGG - chr5.2:21539234-21539253 None:intergenic 30.0%
AGAAACAAGTGAAAAAGATG+TGG + chr5.2:21538286-21538305 MS.gene027824:CDS 30.0%
TGAATTTGAACATCCAAACA+AGG + chr5.2:21538068-21538087 MS.gene027824:CDS 30.0%
TTGTTTGGATGTTCAAATTC+AGG - chr5.2:21538069-21538088 None:intergenic 30.0%
!! ATGTTTTTATAGTCTGCACT+AGG + chr5.2:21539086-21539105 MS.gene027824:intron 30.0%
!! TGAAAGTTTTGACACATCAA+AGG - chr5.2:21538265-21538284 None:intergenic 30.0%
AAATACAGGCATGAGATTGT+TGG + chr5.2:21538483-21538502 MS.gene027824:intron 35.0%
AACCTTCTTGAGAAGTTGAA+AGG - chr5.2:21538135-21538154 None:intergenic 35.0%
AAGGAAGCAAAAGAACACTT+AGG + chr5.2:21538939-21538958 MS.gene027824:CDS 35.0%
ACCTTCTTGAGAAGTTGAAA+GGG - chr5.2:21538134-21538153 None:intergenic 35.0%
ATATGCTAACAATTATCGGC+AGG + chr5.2:21539326-21539345 MS.gene027824:intron 35.0%
CATGGAAAACTTGTAATGGT+TGG + chr5.2:21539182-21539201 MS.gene027824:CDS 35.0%
CCAAAATTAAAGGAAAGACG+GGG - chr5.2:21539232-21539251 None:intergenic 35.0%
CCCAAAATTAAAGGAAAGAC+GGG - chr5.2:21539233-21539252 None:intergenic 35.0%
GATATTTGACATCTGCTTTG+AGG - chr5.2:21539490-21539509 None:intergenic 35.0%
GATTGTTGGTATAGTGACAA+AGG + chr5.2:21538497-21538516 MS.gene027824:CDS 35.0%
GCTAGCAGATGTACTAATTA+CGG - chr5.2:21538913-21538932 None:intergenic 35.0%
GTCTCATGGAAAACTTGTAA+TGG + chr5.2:21539178-21539197 MS.gene027824:CDS 35.0%
TACAGTCCTCTTAGATACTT+TGG + chr5.2:21538792-21538811 MS.gene027824:CDS 35.0%
TACATCTGCTAGCAAAGAAA+AGG + chr5.2:21538920-21538939 MS.gene027824:CDS 35.0%
TATGCTAACAATTATCGGCA+GGG + chr5.2:21539327-21539346 MS.gene027824:intron 35.0%
TTCAATGTTGGAGACAAAGT+AGG + chr5.2:21538540-21538559 MS.gene027824:CDS 35.0%
! GCTAGCTTACCCAAAATTAA+AGG - chr5.2:21539242-21539261 None:intergenic 35.0%
! GTGAAATTTGCCAAAGCTTT+TGG + chr5.2:21538876-21538895 MS.gene027824:CDS 35.0%
! TTATTCTGACTCATTGGTTG+CGG + chr5.2:21538689-21538708 MS.gene027824:CDS 35.0%
!! CCCGTCTTTCCTTTAATTTT+GGG + chr5.2:21539230-21539249 MS.gene027824:CDS 35.0%
!! GATAGGATGATTTGCTGAAA+CGG - chr5.2:21539135-21539154 None:intergenic 35.0%
AATCCAAGTAGTGGCAAGAT+AGG - chr5.2:21539152-21539171 None:intergenic 40.0%
ACCGATTGTAGTAGTGTATG+TGG - chr5.2:21538634-21538653 None:intergenic 40.0%
AGAATAACCTCCGTATGTGA+TGG - chr5.2:21538676-21538695 None:intergenic 40.0%
ATAGTCTGCACTAGGTACTT+TGG + chr5.2:21539094-21539113 MS.gene027824:intron 40.0%
ATCAACCTATCCACTAGTTC+CGG + chr5.2:21538376-21538395 MS.gene027824:CDS 40.0%
CACATTAGCACCAAAAGCTT+TGG - chr5.2:21538889-21538908 None:intergenic 40.0%
CCCACATACACTACTACAAT+CGG + chr5.2:21538630-21538649 MS.gene027824:CDS 40.0%
CCGATTGTAGTAGTGTATGT+GGG - chr5.2:21538633-21538652 None:intergenic 40.0%
CCTTCTTGAGAAGTTGAAAG+GGG - chr5.2:21538133-21538152 None:intergenic 40.0%
CGATTGTAGTAGTGTATGTG+GGG - chr5.2:21538632-21538651 None:intergenic 40.0%
CTTGGATTACTGAAGTCTCA+TGG + chr5.2:21539164-21539183 MS.gene027824:CDS 40.0%
GACTTCAGTAATCCAAGTAG+TGG - chr5.2:21539161-21539180 None:intergenic 40.0%
GTTGGTATAGTGACAAAGGT+GGG + chr5.2:21538501-21538520 MS.gene027824:CDS 40.0%
TCGTGACCAAGATAAAATGC+AGG + chr5.2:21538983-21539002 MS.gene027824:CDS 40.0%
TGTTGGTATAGTGACAAAGG+TGG + chr5.2:21538500-21538519 MS.gene027824:CDS 40.0%
! GACAAACCTGGTCTTCATAT+CGG + chr5.2:21538819-21538838 MS.gene027824:CDS 40.0%
! TCGAGTCCAAAGTATCTAAG+AGG - chr5.2:21538801-21538820 None:intergenic 40.0%
!! AAGTGGAATTGGAAGCATCA+AGG + chr5.2:21539367-21539386 MS.gene027824:CDS 40.0%
!! CCCCGTCTTTCCTTTAATTT+TGG + chr5.2:21539229-21539248 MS.gene027824:CDS 40.0%
!! TATCGATTTGTGCTCGACAT+TGG + chr5.2:21539506-21539525 MS.gene027824:CDS 40.0%
ACAACACCGATATGAAGACC+AGG - chr5.2:21538828-21538847 None:intergenic 45.0%
ACAGGAACGACTTCAATGTC+AGG - chr5.2:21539437-21539456 None:intergenic 45.0%
ATCCAAACAAGGCCTTTGGT+TGG + chr5.2:21538079-21538098 MS.gene027824:CDS 45.0%
ATGTCACTCAGATGTCTGCA+TGG + chr5.2:21538334-21538353 MS.gene027824:CDS 45.0%
CATCCTATCTTGCCACTACT+TGG + chr5.2:21539146-21539165 MS.gene027824:CDS 45.0%
CCCCTTTCAACTTCTCAAGA+AGG + chr5.2:21538130-21538149 MS.gene027824:CDS 45.0%
CGGAGGTTATTCTGACTCAT+TGG + chr5.2:21538683-21538702 MS.gene027824:CDS 45.0%
GAACATCCAAACAAGGCCTT+TGG + chr5.2:21538075-21538094 MS.gene027824:CDS 45.0%
GCTGTGTTCACATAATCCAC+AGG - chr5.2:21539455-21539474 None:intergenic 45.0%
GTTGGAGACAAAGTAGGTGT+TGG + chr5.2:21538546-21538565 MS.gene027824:CDS 45.0%
TACGTGGTTCACATTCCTGA+TGG + chr5.2:21538720-21538739 MS.gene027824:CDS 45.0%
TACTTTGGACTCGACAAACC+TGG + chr5.2:21538807-21538826 MS.gene027824:CDS 45.0%
TCAACCTATCCACTAGTTCC+GGG + chr5.2:21538377-21538396 MS.gene027824:CDS 45.0%
TCCAAACAAGGCCTTTGGTT+GGG + chr5.2:21538080-21538099 MS.gene027824:CDS 45.0%
TCTGCACTAGGTACTTTGGA+TGG + chr5.2:21539098-21539117 MS.gene027824:CDS 45.0%
TGACATTGAAGTCGTTCCTG+TGG + chr5.2:21539436-21539455 MS.gene027824:CDS 45.0%
! CGTGATGGAACCATCACATA+CGG + chr5.2:21538663-21538682 MS.gene027824:CDS 45.0%
!! CATATCGGTGTTGTTGGTCT+TGG + chr5.2:21538834-21538853 MS.gene027824:CDS 45.0%
!! GGTCTTCATATCGGTGTTGT+TGG + chr5.2:21538828-21538847 MS.gene027824:CDS 45.0%
ACACAGTGATCCCAGCACAA+AGG - chr5.2:21538773-21538792 None:intergenic 50.0%
AGAAAGCTGGTAGCTGGAAG+TGG + chr5.2:21539350-21539369 MS.gene027824:CDS 50.0%
ATTATCGGCAGGGAGAAAGC+TGG + chr5.2:21539337-21539356 MS.gene027824:intron 50.0%
CAACCTATCCACTAGTTCCG+GGG + chr5.2:21538378-21538397 MS.gene027824:CDS 50.0%
CTGCATGGCGAAGAACGAAT+GGG + chr5.2:21538349-21538368 MS.gene027824:CDS 50.0%
CTGGTAGCTGGAAGTGGAAT+TGG + chr5.2:21539356-21539375 MS.gene027824:CDS 50.0%
GATGGAACCATCACATACGG+AGG + chr5.2:21538666-21538685 MS.gene027824:CDS 50.0%
GATGGTTCCATCACGATGCT+TGG - chr5.2:21538658-21538677 None:intergenic 50.0%
GCTTCAAGTGGTAAGCCATC+AGG - chr5.2:21538738-21538757 None:intergenic 50.0%
TCTGCATGGCGAAGAACGAA+TGG + chr5.2:21538348-21538367 MS.gene027824:CDS 50.0%
TGCATGGCGAAGAACGAATG+GGG + chr5.2:21538350-21538369 MS.gene027824:CDS 50.0%
! ATACCCCGGAACTAGTGGAT+AGG - chr5.2:21538384-21538403 None:intergenic 50.0%
!! ATCGGTGTTGTTGGTCTTGG+TGG + chr5.2:21538837-21538856 MS.gene027824:CDS 50.0%
!! TCGGTGTTGTTGGTCTTGGT+GGG + chr5.2:21538838-21538857 MS.gene027824:CDS 50.0%
AGAGCATACCCCGGAACTAG+TGG - chr5.2:21538389-21538408 None:intergenic 55.0%
CTGCACCTCTCCTTTGTGCT+GGG + chr5.2:21538760-21538779 MS.gene027824:CDS 55.0%
GGTTGCGGATGAGAACTACG+TGG + chr5.2:21538704-21538723 MS.gene027824:CDS 55.0%
GTCTCTAGCTGCCCAACCAA+AGG - chr5.2:21538094-21538113 None:intergenic 55.0%
GTGATCCCAGCACAAAGGAG+AGG - chr5.2:21538768-21538787 None:intergenic 55.0%
TGGGCAGCTAGAGACACATC+TGG + chr5.2:21538099-21538118 MS.gene027824:CDS 55.0%
TGGTGTACCTGACAAGCCTC+TGG + chr5.2:21539202-21539221 MS.gene027824:CDS 55.0%
! AGAGGTGCAGCAGCTTCAAG+TGG - chr5.2:21538750-21538769 None:intergenic 55.0%
!! ATCGGTGCCAAGCATCGTGA+TGG + chr5.2:21538648-21538667 MS.gene027824:CDS 55.0%
!! GCCCAACCAAAGGCCTTGTT+TGG - chr5.2:21538084-21538103 None:intergenic 55.0%
!! GTTGTTGGTCTTGGTGGGCT+TGG + chr5.2:21538843-21538862 MS.gene027824:CDS 55.0%
!! TCTTGGTGGGCTTGGTCACA+TGG + chr5.2:21538851-21538870 MS.gene027824:CDS 55.0%
GCAGGGAGAAAGCTGGTAGC+TGG + chr5.2:21539344-21539363 MS.gene027824:intron 60.0%
GCTGCACCTCTCCTTTGTGC+TGG + chr5.2:21538759-21538778 MS.gene027824:CDS 60.0%
GGAAAGACGGGGATCTCCAG+AGG - chr5.2:21539221-21539240 None:intergenic 60.0%
GGGATCTCCAGAGGCTTGTC+AGG - chr5.2:21539212-21539231 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 21538054 21539553 21538054 ID=MS.gene027824
chr5.2 mRNA 21538054 21539553 21538054 ID=MS.gene027824.t1;Parent=MS.gene027824
chr5.2 exon 21538054 21538151 21538054 ID=MS.gene027824.t1.exon1;Parent=MS.gene027824.t1
chr5.2 CDS 21538054 21538151 21538054 ID=cds.MS.gene027824.t1;Parent=MS.gene027824.t1
chr5.2 exon 21538286 21538399 21538286 ID=MS.gene027824.t1.exon2;Parent=MS.gene027824.t1
chr5.2 CDS 21538286 21538399 21538286 ID=cds.MS.gene027824.t1;Parent=MS.gene027824.t1
chr5.2 exon 21538491 21539004 21538491 ID=MS.gene027824.t1.exon3;Parent=MS.gene027824.t1
chr5.2 CDS 21538491 21539004 21538491 ID=cds.MS.gene027824.t1;Parent=MS.gene027824.t1
chr5.2 exon 21539098 21539251 21539098 ID=MS.gene027824.t1.exon4;Parent=MS.gene027824.t1
chr5.2 CDS 21539098 21539251 21539098 ID=cds.MS.gene027824.t1;Parent=MS.gene027824.t1
chr5.2 exon 21539348 21539553 21539348 ID=MS.gene027824.t1.exon5;Parent=MS.gene027824.t1
chr5.2 CDS 21539348 21539553 21539348 ID=cds.MS.gene027824.t1;Parent=MS.gene027824.t1
Gene Sequence

>MS.gene027824

ATGGCAACACAACCTGAATTTGAACATCCAAACAAGGCCTTTGGTTGGGCAGCTAGAGACACATCTGGTGTTCTCTCCCCTTTCAACTTCTCAAGAAGGTTCATTCATACACCACTTCACTTTTTTTTCAATTAATTTATTTTTTACACTTTGATGCATCATATATAGTGTTAAGAGTAAAATTGTTGTTAGGAAAATATATAACTCACCTTTGATGTGTCAAAACTTTCAGAGAAACAAGTGAAAAAGATGTGGCATTCAAAATATTGTATTGTGGAATATGTCACTCAGATGTCTGCATGGCGAAGAACGAATGGGGCATATCAACCTATCCACTAGTTCCGGGGTATGCTCTAATTAAATTATCTTAGAAAAGTCTTAAAGTCTTACCATTTTCTACTATTTTGATTTGATTCTAATTTATATAAAAAATACAGGCATGAGATTGTTGGTATAGTGACAAAGGTGGGAAGCAAAGTAGAAAAGTTCAATGTTGGAGACAAAGTAGGTGTTGGCTACATAATTGATTCATGTCGATCGTGTCAAAATTGTGTTGACAATCTTGAAAATTACTGCCCCACATACACTACTACAATCGGTGCCAAGCATCGTGATGGAACCATCACATACGGAGGTTATTCTGACTCATTGGTTGCGGATGAGAACTACGTGGTTCACATTCCTGATGGCTTACCACTTGAAGCTGCTGCACCTCTCCTTTGTGCTGGGATCACTGTGTACAGTCCTCTTAGATACTTTGGACTCGACAAACCTGGTCTTCATATCGGTGTTGTTGGTCTTGGTGGGCTTGGTCACATGGCTGTGAAATTTGCCAAAGCTTTTGGTGCTAATGTGACCGTAATTAGTACATCTGCTAGCAAAGAAAAGGAAGCAAAAGAACACTTAGGAGCTGATTCATTTCTGATAAGTCGTGACCAAGATAAAATGCAGGTATAAAAACAATAGTTCAATATTCTATTATACGGAAATTTACAATGCTATTCCCAATCAATTTATGAACTAATTATGTTAATGTTTTTATAGTCTGCACTAGGTACTTTGGATGGTATCATTGATACCGTTTCAGCAAATCATCCTATCTTGCCACTACTTGGATTACTGAAGTCTCATGGAAAACTTGTAATGGTTGGTGTACCTGACAAGCCTCTGGAGATCCCCGTCTTTCCTTTAATTTTGGGTAAGCTAGCATATATATGAACTACGTTTCCATAGATCACTTATATGATTAAAAGCTACAAGTCATATATACTTATATGCTAACAATTATCGGCAGGGAGAAAGCTGGTAGCTGGAAGTGGAATTGGAAGCATCAAGGAGACTCAAGAGATGCTTGACTTTGCTGCTAAACACAATGTAAAACCTGACATTGAAGTCGTTCCTGTGGATTATGTGAACACAGCAATTGAGCGACTCCTCAAAGCAGATGTCAAATATCGATTTGTGCTCGACATTGGAAACACTCTGAAACCAAGTTCTTAA
Protein sequence

>MS.gene027824.t1

MATQPEFEHPNKAFGWAARDTSGVLSPFNFSRRETSEKDVAFKILYCGICHSDVCMAKNEWGISTYPLVPGHEIVGIVTKVGSKVEKFNVGDKVGVGYIIDSCRSCQNCVDNLENYCPTYTTTIGAKHRDGTITYGGYSDSLVADENYVVHIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGLHIGVVGLGGLGHMAVKFAKAFGANVTVISTSASKEKEAKEHLGADSFLISRDQDKMQSALGTLDGIIDTVSANHPILPLLGLLKSHGKLVMVGVPDKPLEIPVFPLILGRKLVAGSGIGSIKETQEMLDFAAKHNVKPDIEVVPVDYVNTAIERLLKADVKYRFVLDIGNTLKPSS