AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01230.t1 MTR_2g009810 97.183 355 10 0 1 355 52 406 0.0 716
MS.gene01230.t1 MTR_2g009820 89.113 248 23 2 112 355 7 254 1.70e-149 422
MS.gene01230.t1 MTR_5g031190 53.582 349 160 2 8 355 11 358 3.39e-124 362
MS.gene01230.t1 MTR_5g031360 54.155 349 158 2 8 355 13 360 1.34e-123 360
MS.gene01230.t1 MTR_5g031230 54.310 348 153 4 11 355 14 358 7.52e-120 350
MS.gene01230.t1 MTR_5g031310 53.314 347 158 3 11 355 15 359 7.02e-119 348
MS.gene01230.t1 MTR_5g031305 53.602 347 157 3 11 355 15 359 2.04e-118 347
MS.gene01230.t1 MTR_5g031390 52.450 347 161 3 11 355 14 358 3.92e-118 346
MS.gene01230.t1 MTR_5g031210 54.335 346 156 2 11 355 14 358 6.47e-118 345
MS.gene01230.t1 MTR_5g031250 52.312 346 163 2 11 355 14 358 5.07e-117 343
MS.gene01230.t1 MTR_4g115300 53.026 347 159 3 11 355 14 358 5.91e-117 343
MS.gene01230.t1 MTR_5g031180 54.913 346 154 2 11 355 14 358 1.76e-116 342
MS.gene01230.t1 MTR_5g031300 51.576 349 167 2 8 355 12 359 6.01e-116 340
MS.gene01230.t1 MTR_8g068190 54.335 346 156 2 11 355 14 358 5.98e-115 338
MS.gene01230.t1 MTR_8g092630 55.362 345 151 3 11 354 71 413 7.78e-114 337
MS.gene01230.t1 MTR_5g031430 51.437 348 163 3 11 355 14 358 1.58e-111 329
MS.gene01230.t1 MTR_1g025950 52.478 343 160 3 13 353 15 356 7.71e-110 325
MS.gene01230.t1 MTR_1g107425 45.070 355 192 3 1 353 1 354 7.15e-101 302
MS.gene01230.t1 MTR_1g107425 45.791 297 158 3 1 295 1 296 2.16e-80 248
MS.gene01230.t1 MTR_5g031250 51.653 242 112 2 11 248 14 254 1.19e-74 231
MS.gene01230.t1 MTR_5g031290 55.357 112 49 1 245 355 80 191 1.09e-30 115
MS.gene01230.t1 MTR_5g031290 52.857 70 32 1 8 76 11 80 7.74e-18 80.9
MS.gene01230.t1 MTR_8g095220 24.062 320 209 8 16 305 60 375 3.06e-17 82.8
MS.gene01230.t1 MTR_8g095220 25.911 247 161 5 16 242 60 304 3.44e-16 78.6
MS.gene01230.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.05e-13 71.2
MS.gene01230.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.35e-13 70.9
MS.gene01230.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.70e-13 70.1
MS.gene01230.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.87e-13 70.1
MS.gene01230.t1 MTR_0001s0660 26.280 293 190 9 34 304 34 322 3.96e-13 70.5
MS.gene01230.t1 MTR_3g089977 23.973 292 198 7 36 304 37 327 7.61e-13 69.3
MS.gene01230.t1 MTR_3g089970 23.698 384 256 11 1 351 1 380 8.00e-13 69.3
MS.gene01230.t1 MTR_8g064610 30.102 196 107 5 57 251 128 294 9.22e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01230.t1 AT1G72680 75.706 354 85 1 2 355 3 355 0.0 555
MS.gene01230.t1 AT4G37970 50.144 347 171 2 9 354 13 358 9.23e-114 335
MS.gene01230.t1 AT2G21890 52.874 348 157 4 11 354 8 352 1.63e-113 335
MS.gene01230.t1 AT4G39330 51.884 345 164 2 11 354 14 357 2.86e-113 334
MS.gene01230.t1 AT2G21730 52.738 347 160 4 11 354 8 353 2.67e-112 332
MS.gene01230.t1 AT4G37980 49.284 349 175 2 8 355 7 354 5.94e-104 310
MS.gene01230.t1 AT4G37990 48.138 349 179 2 8 355 7 354 1.40e-98 296
MS.gene01230.t1 AT3G19450 45.070 355 192 3 1 353 1 354 1.83e-91 278
MS.gene01230.t1 AT4G39330 51.034 290 140 2 11 299 14 302 1.79e-89 271
MS.gene01230.t1 AT4G34230 45.906 342 183 2 13 353 13 353 2.08e-88 270
MS.gene01230.t1 AT4G34230 45.906 342 183 2 13 353 13 353 2.70e-88 270
MS.gene01230.t1 AT4G37970 49.291 282 141 2 9 290 13 292 9.24e-87 264
MS.gene01230.t1 AT4G37980 49.817 273 136 1 8 280 7 278 1.18e-79 246
MS.gene01230.t1 AT4G37970 51.293 232 112 1 9 240 13 243 3.44e-75 233
MS.gene01230.t1 AT4G37970 51.293 232 112 1 9 240 13 243 3.87e-75 233
MS.gene01230.t1 AT5G63620 26.506 249 157 6 16 242 62 306 6.30e-17 80.9
MS.gene01230.t1 AT5G63620 25.225 333 215 9 16 318 62 390 8.35e-17 81.6
MS.gene01230.t1 AT5G63620 25.225 333 215 9 16 318 62 390 8.91e-17 81.6
MS.gene01230.t1 AT5G43940 26.222 225 135 4 36 238 36 251 9.33e-14 72.0
MS.gene01230.t1 AT5G43940 26.222 225 135 4 36 238 48 263 9.41e-14 72.4
MS.gene01230.t1 AT1G32780 25.552 317 188 10 36 312 37 345 3.44e-13 70.5
MS.gene01230.t1 AT1G77120 25.589 297 187 9 36 304 36 326 3.79e-12 67.4

Find 106 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCATATGGCAGTAAAATT+TGG 0.150937 2.4:+73368746 MS.gene01230:CDS
ATTGGAATCAGTTCTATTTC+TGG 0.200827 2.4:-73369341 None:intergenic
ATCATAATCAACACCATTAA+AGG 0.217231 2.4:-73368358 None:intergenic
GGATCAGTTTACACCTTTAA+TGG 0.243928 2.4:+73368345 MS.gene01230:CDS
TTTGTTATGCTGATGTAATT+TGG 0.289646 2.4:+73368044 MS.gene01230:CDS
GAGGAGGCGCTGAGCCTTCT+TGG 0.291889 2.4:+73368821 MS.gene01230:CDS
TCTGGTGTTCTGGTCTTAGT+TGG 0.292846 2.4:+73369089 MS.gene01230:CDS
TTACCTAAGTTAAGGCTTGC+AGG 0.313929 2.4:-73369137 None:intergenic
GTAAAATTTGGGAAGGCATT+TGG 0.314204 2.4:+73368758 MS.gene01230:CDS
TCTTGAGAGGGTCGTAAATA+AGG 0.324188 2.4:+73369382 MS.gene01230:CDS
TCTGAAGATATCTGGTGTTC+TGG 0.326227 2.4:+73369079 MS.gene01230:CDS
TTGTTATGCTGATGTAATTT+GGG 0.354641 2.4:+73368045 MS.gene01230:CDS
TGTACCACCTGCAACACTTC+CGG 0.359100 2.4:-73369270 None:intergenic
ACCTTGAAACGCTGGACATT+AGG 0.363094 2.4:-73368234 None:intergenic
CTTATCAGACACGAGATTGC+TGG 0.363214 2.4:+73368192 MS.gene01230:intron
GTCATATGGCAGTAAAATTT+GGG 0.370755 2.4:+73368747 MS.gene01230:CDS
TTGGAATCAGTTCTATTTCT+GGG 0.372653 2.4:-73369340 None:intergenic
GCAGGTCCTTTGCTTTGTGC+TGG 0.376092 2.4:+73368641 MS.gene01230:CDS
GATTAGATGAAACAACAAAT+TGG 0.376372 2.4:-73368850 None:intergenic
GAAATAGAACTGATTCCAAT+TGG 0.379523 2.4:+73369344 MS.gene01230:CDS
TCTCTTGGAGTGGTTGGTCT+TGG 0.380973 2.4:+73368716 MS.gene01230:CDS
CTGGTGTTCTGGTCTTAGTT+GGG 0.395199 2.4:+73369090 MS.gene01230:CDS
GTCGACTTCTGTGCTGCAAA+TGG 0.396290 2.4:+73369311 MS.gene01230:CDS
CTTGGAGTGGTTGGTCTTGG+TGG 0.399951 2.4:+73368719 MS.gene01230:CDS
TCTTGGTGGCCTCGGTCATA+TGG 0.406264 2.4:+73368733 MS.gene01230:CDS
GCAGGCGTTGGCTAAATCAT+TGG 0.409444 2.4:+73369001 MS.gene01230:intron
ATGACACTTCTGAAGATATC+TGG 0.409523 2.4:+73369071 MS.gene01230:CDS
ATGCAACATTACCTAAGTTA+AGG 0.414851 2.4:-73369145 None:intergenic
TTTATAATTGACACGGCATC+GGG 0.416518 2.4:+73369026 MS.gene01230:CDS
TCAGGATCAAGAACTGTTGC+CGG 0.416934 2.4:+73369251 MS.gene01230:intron
GCTTCATTTGAGTACCCAAT+TGG 0.417911 2.4:-73369359 None:intergenic
TAAATAAGGACGTGAAATAT+CGG 0.419184 2.4:+73369396 MS.gene01230:CDS
TACCTGCGATTGAATTTGTA+AGG 0.426854 2.4:-73367517 None:intergenic
GGGCTGAATTTGACTTCACT+TGG 0.427549 2.4:-73369116 None:intergenic
ACTGTTGCCGGAAGTGTTGC+AGG 0.432996 2.4:+73369263 MS.gene01230:CDS
ACAACTTACCCCGGCACAAC+AGG 0.440053 2.4:-73368097 None:intergenic
TCCTAATGTCCAGCGTTTCA+AGG 0.445977 2.4:+73368233 MS.gene01230:CDS
ATTTCTCTAATTCTATTTGA+GGG 0.446898 2.4:-73369441 None:intergenic
TATTTCTCTAATTCTATTTG+AGG 0.449373 2.4:-73369442 None:intergenic
GTGGTTGGTCTTGGTGGCCT+CGG 0.451789 2.4:+73368725 MS.gene01230:CDS
TTCAAAGTATCCTGTTGTGC+CGG 0.454354 2.4:+73368087 MS.gene01230:CDS
AATCAACCTGGTAAGTCTCT+TGG 0.459390 2.4:+73368701 MS.gene01230:CDS
TCAAAGTATCCTGTTGTGCC+GGG 0.460698 2.4:+73368088 MS.gene01230:CDS
AGCTATCCTTTAGCTTCAGC+AGG 0.468468 2.4:+73368623 MS.gene01230:CDS
ACACGAGATTGCTGGGGTTG+TGG 0.469150 2.4:+73368200 MS.gene01230:CDS
GCTGGGGTTGTGGCAAAGGT+TGG 0.470558 2.4:+73368210 MS.gene01230:CDS
ATTCATATTATGTCGCATCA+TGG 0.471650 2.4:-73368681 None:intergenic
TTATCAGACACGAGATTGCT+GGG 0.475272 2.4:+73368193 MS.gene01230:intron
GGCATTTGGTTTGCGTGTAA+CGG 0.491251 2.4:+73368772 MS.gene01230:CDS
CGCCTTACAAATTCAATCGC+AGG 0.493938 2.4:+73367515 MS.gene01230:CDS
ATCTTCAGAAGTGTCATGTA+AGG 0.496050 2.4:-73369065 None:intergenic
AGGTGGTACAAAAGAAATAC+AGG 0.497345 2.4:+73369283 MS.gene01230:CDS
GGTAAGTCTCTTGGAGTGGT+TGG 0.503337 2.4:+73368710 MS.gene01230:CDS
TGGTGATTGCTAAGGGAGCT+CGG 0.505418 2.4:+73367983 MS.gene01230:intron
TATGATGGTACAATTACAAA+AGG 0.507275 2.4:+73368375 MS.gene01230:CDS
TGGGCAGCAAGAGATGCATC+TGG 0.511542 2.4:+73367484 MS.gene01230:CDS
TTTAATGGTGTTGATTATGA+TGG 0.517784 2.4:+73368360 MS.gene01230:CDS
TCGACTTCTGTGCTGCAAAT+GGG 0.518039 2.4:+73369312 MS.gene01230:CDS
GGTGATTGCTAAGGGAGCTC+GGG 0.518767 2.4:+73367984 MS.gene01230:intron
CATCTCTTGCTGCCCATGCA+AGG 0.525887 2.4:-73367477 None:intergenic
TTGGTGAAGATTGCCTTGCA+TGG 0.528700 2.4:+73367464 MS.gene01230:CDS
TTCAAGGTTGGTGACCATGT+TGG 0.531246 2.4:+73368249 MS.gene01230:CDS
AGTGTCATGTAAGGATCAAA+CGG 0.531817 2.4:-73369056 None:intergenic
CACTAGCATTTCAAAGAAAG+AGG 0.533511 2.4:+73368802 MS.gene01230:CDS
AAATAGAACTGATTCCAATT+GGG 0.533719 2.4:+73369345 MS.gene01230:CDS
AAAGGACCTGCTGAAGCTAA+AGG 0.536529 2.4:-73368629 None:intergenic
AATGTCCAGCGTTTCAAGGT+TGG 0.539954 2.4:+73368237 MS.gene01230:CDS
GATTGCTGGGGTTGTGGCAA+AGG 0.541441 2.4:+73368206 MS.gene01230:CDS
GGCTGAATTTGACTTCACTT+GGG 0.543028 2.4:-73369115 None:intergenic
TTCATATTATGTCGCATCAT+GGG 0.544385 2.4:-73368680 None:intergenic
TACCTAAGTTAAGGCTTGCA+GGG 0.544423 2.4:-73369136 None:intergenic
TGGTGAAGATTGCCTTGCAT+GGG 0.548223 2.4:+73367465 MS.gene01230:CDS
TACAAAAGAAATACAGGAGA+TGG 0.550321 2.4:+73369289 MS.gene01230:CDS
ATTAGAAGTTCATTGTGTGA+AGG 0.552235 2.4:+73368323 MS.gene01230:CDS
TTAGAAGTTCATTGTGTGAA+GGG 0.558615 2.4:+73368324 MS.gene01230:CDS
GTACTTACATCAACTCATGC+AGG 0.564363 2.4:+73368277 MS.gene01230:CDS
GTGTCATGTAAGGATCAAAC+GGG 0.564881 2.4:-73369055 None:intergenic
CGACATAATATGAATCAACC+TGG 0.568407 2.4:+73368689 MS.gene01230:CDS
AGCCCTGCAAGCCTTAACTT+AGG 0.570562 2.4:+73369134 MS.gene01230:CDS
TTACCTTTCATGTACTACAA+TGG 0.576358 2.4:-73368412 None:intergenic
CATCTAATCAAGAAGAGATG+AGG 0.579627 2.4:+73368864 MS.gene01230:CDS
CTTCCATTGTAGTACATGAA+AGG 0.579906 2.4:+73368409 MS.gene01230:CDS
CAAATTGGTCTGCACCAAGA+AGG 0.580444 2.4:-73368835 None:intergenic
GTGATTCCAGCACAAAGCAA+AGG 0.590358 2.4:-73368647 None:intergenic
GGTCACCAACCTTGAAACGC+TGG 0.591766 2.4:-73368242 None:intergenic
ATCTAATCAAGAAGAGATGA+GGG 0.596139 2.4:+73368865 MS.gene01230:CDS
ACTCAAATGAAGCTCTTGAG+AGG 0.606095 2.4:+73369369 MS.gene01230:CDS
TTTGGGCAAGGAATAAACAT+GGG 0.615486 2.4:+73368062 MS.gene01230:CDS
ACCTGGTAAGTCTCTTGGAG+TGG 0.618165 2.4:+73368706 MS.gene01230:CDS
TATGGCAGTAAAATTTGGGA+AGG 0.622661 2.4:+73368751 MS.gene01230:CDS
CTCAAATGAAGCTCTTGAGA+GGG 0.622942 2.4:+73369370 MS.gene01230:CDS
GATGGTACAATTACAAAAGG+AGG 0.626222 2.4:+73368378 MS.gene01230:CDS
ATTTGGGCAAGGAATAAACA+TGG 0.627500 2.4:+73368061 MS.gene01230:CDS
ATGCTGATGTAATTTGGGCA+AGG 0.629018 2.4:+73368050 MS.gene01230:CDS
TACTTACATCAACTCATGCA+GGG 0.634667 2.4:+73368278 MS.gene01230:CDS
CAAAGTATCCTGTTGTGCCG+GGG 0.639115 2.4:+73368089 MS.gene01230:CDS
GGTTGGTGACCATGTTGGAG+TGG 0.639841 2.4:+73368254 MS.gene01230:CDS
TTGGGCAAGGAATAAACATG+GGG 0.641841 2.4:+73368063 MS.gene01230:CDS
TATGTTAAAATTACACACTG+TGG 0.642017 2.4:+73368019 MS.gene01230:CDS
GTTGGTGACCATGTTGGAGT+GGG 0.658549 2.4:+73368255 MS.gene01230:CDS
TGTAAGTACCCACTCCAACA+TGG 0.660381 2.4:-73368263 None:intergenic
ACCACTCCAAGAGACTTACC+AGG 0.671497 2.4:-73368707 None:intergenic
TAGCATTTCAAAGAAAGAGG+AGG 0.681888 2.4:+73368805 MS.gene01230:CDS
TATCAGACACGAGATTGCTG+GGG 0.696618 2.4:+73368194 MS.gene01230:intron
GCTATGAGTTCTGAAAGTGT+TGG 0.742932 2.4:+73367445 None:intergenic
GTTGCCGGAAGTGTTGCAGG+TGG 0.789484 2.4:+73369266 MS.gene01230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAGGATTTAATTTTTTTTT+TGG - chr2.4:73367952-73367971 None:intergenic 15.0%
!!! TTCATAACTTCTTTTATATA+TGG + chr2.4:73368564-73368583 MS.gene01230:intron 15.0%
!!! AAATGTTTCTAATGACTTAA+CGG - chr2.4:73367658-73367677 None:intergenic 20.0%
!!! ATGAGATTTTACTTAAAACT+CGG - chr2.4:73367777-73367796 None:intergenic 20.0%
!!! ATTGGATTTTATAATTGACA+CGG + chr2.4:73369019-73369038 MS.gene01230:CDS 20.0%
!!! CTTCTTTGATTTCATAATAA+TGG + chr2.4:73368522-73368541 MS.gene01230:intron 20.0%
!!! TGTTTTTTTAACTAAACTGA+CGG + chr2.4:73368968-73368987 MS.gene01230:intron 20.0%
!!! TTTTTTGGAAAAATGTAACA+TGG - chr2.4:73367937-73367956 None:intergenic 20.0%
! AAAGAAAAACACAGATAAGT+AGG - chr2.4:73368169-73368188 None:intergenic 25.0%
! AAATCCTGACAATATGATAA+TGG + chr2.4:73367963-73367982 MS.gene01230:intron 25.0%
! AATGCATACATTCTTATATG+TGG - chr2.4:73367897-73367916 None:intergenic 25.0%
! ATATATACTGTCAGTGTAAA+GGG + chr2.4:73367606-73367625 MS.gene01230:intron 25.0%
! ATCATAATCAACACCATTAA+AGG - chr2.4:73368361-73368380 None:intergenic 25.0%
! ATGCATACATTCTTATATGT+GGG - chr2.4:73367896-73367915 None:intergenic 25.0%
! GATTAGATGAAACAACAAAT+TGG - chr2.4:73368853-73368872 None:intergenic 25.0%
! TAAATAAGGACGTGAAATAT+CGG + chr2.4:73369396-73369415 MS.gene01230:CDS 25.0%
! TAATAAATTTCAGCAAGAGA+AGG - chr2.4:73368487-73368506 None:intergenic 25.0%
! TATATATACTGTCAGTGTAA+AGG + chr2.4:73367605-73367624 MS.gene01230:intron 25.0%
! TATGATGGTACAATTACAAA+AGG + chr2.4:73368375-73368394 MS.gene01230:CDS 25.0%
! TATGTTAAAATTACACACTG+TGG + chr2.4:73368019-73368038 MS.gene01230:CDS 25.0%
! TGAAAGGTAAACTTAATAGA+TGG + chr2.4:73368425-73368444 MS.gene01230:intron 25.0%
! TTGTTATGCTGATGTAATTT+GGG + chr2.4:73368045-73368064 MS.gene01230:CDS 25.0%
!! AAATAGAACTGATTCCAATT+GGG + chr2.4:73369345-73369364 MS.gene01230:CDS 25.0%
!! CTTTGATTTCATAATAATGG+AGG + chr2.4:73368525-73368544 MS.gene01230:intron 25.0%
!! TTGAATGGAGTAATTCTTTT+AGG + chr2.4:73367570-73367589 MS.gene01230:intron 25.0%
!! TTTGTTATGCTGATGTAATT+TGG + chr2.4:73368044-73368063 MS.gene01230:CDS 25.0%
!!! TTTAATGGTGTTGATTATGA+TGG + chr2.4:73368360-73368379 MS.gene01230:CDS 25.0%
AGAAAATGCTATGGTATAGA+TGG - chr2.4:73368906-73368925 None:intergenic 30.0%
ATCACCATTATCATATTGTC+AGG - chr2.4:73367970-73367989 None:intergenic 30.0%
ATCTAATCAAGAAGAGATGA+GGG + chr2.4:73368865-73368884 MS.gene01230:CDS 30.0%
ATGCAACATTACCTAAGTTA+AGG - chr2.4:73369148-73369167 None:intergenic 30.0%
ATGGTTTGAAGAAAATGCTA+TGG - chr2.4:73368915-73368934 None:intergenic 30.0%
ATTAGAAGTTCATTGTGTGA+AGG + chr2.4:73368323-73368342 MS.gene01230:CDS 30.0%
ATTCATATTATGTCGCATCA+TGG - chr2.4:73368684-73368703 None:intergenic 30.0%
ATTGGAATCAGTTCTATTTC+TGG - chr2.4:73369344-73369363 None:intergenic 30.0%
GTCATATGGCAGTAAAATTT+GGG + chr2.4:73368747-73368766 MS.gene01230:CDS 30.0%
TACAAAAGAAATACAGGAGA+TGG + chr2.4:73369289-73369308 MS.gene01230:CDS 30.0%
TGAATGAAGGATATTACTTC+TGG - chr2.4:73369224-73369243 None:intergenic 30.0%
TTACCTTTCATGTACTACAA+TGG - chr2.4:73368415-73368434 None:intergenic 30.0%
TTAGAAGTTCATTGTGTGAA+GGG + chr2.4:73368324-73368343 MS.gene01230:CDS 30.0%
TTCATATTATGTCGCATCAT+GGG - chr2.4:73368683-73368702 None:intergenic 30.0%
! GAAATAGAACTGATTCCAAT+TGG + chr2.4:73369344-73369363 MS.gene01230:CDS 30.0%
! TTGGAATCAGTTCTATTTCT+GGG - chr2.4:73369343-73369362 None:intergenic 30.0%
! TTTTATAATTGACACGGCAT+CGG + chr2.4:73369025-73369044 MS.gene01230:CDS 30.0%
!! ATGATAATGGTGATTGCTAA+GGG + chr2.4:73367976-73367995 MS.gene01230:intron 30.0%
!! CTCATTTTCCTTTTGTTTCA+AGG + chr2.4:73367792-73367811 MS.gene01230:intron 30.0%
!! TATGATAATGGTGATTGCTA+AGG + chr2.4:73367975-73367994 MS.gene01230:intron 30.0%
AGGTGGTACAAAAGAAATAC+AGG + chr2.4:73369283-73369302 MS.gene01230:CDS 35.0%
AGTGTCATGTAAGGATCAAA+CGG - chr2.4:73369059-73369078 None:intergenic 35.0%
ATTCAACAAAGCAGAATGAG+AGG - chr2.4:73367556-73367575 None:intergenic 35.0%
ATTTGGGCAAGGAATAAACA+TGG + chr2.4:73368061-73368080 MS.gene01230:CDS 35.0%
CACTAGCATTTCAAAGAAAG+AGG + chr2.4:73368802-73368821 MS.gene01230:CDS 35.0%
CAGTATCAGTATTCTCTTCA+TGG - chr2.4:73368934-73368953 None:intergenic 35.0%
CATCTAATCAAGAAGAGATG+AGG + chr2.4:73368864-73368883 MS.gene01230:CDS 35.0%
CGACATAATATGAATCAACC+TGG + chr2.4:73368689-73368708 MS.gene01230:CDS 35.0%
CTTCCATTGTAGTACATGAA+AGG + chr2.4:73368409-73368428 MS.gene01230:CDS 35.0%
GATGGTACAATTACAAAAGG+AGG + chr2.4:73368378-73368397 MS.gene01230:CDS 35.0%
GGATCAGTTTACACCTTTAA+TGG + chr2.4:73368345-73368364 MS.gene01230:CDS 35.0%
GGTAAACTTAATAGATGGCT+TGG + chr2.4:73368430-73368449 MS.gene01230:intron 35.0%
GGTCATATGGCAGTAAAATT+TGG + chr2.4:73368746-73368765 MS.gene01230:CDS 35.0%
TACCTGCGATTGAATTTGTA+AGG - chr2.4:73367520-73367539 None:intergenic 35.0%
TACTTACATCAACTCATGCA+GGG + chr2.4:73368278-73368297 MS.gene01230:CDS 35.0%
TAGCATTTCAAAGAAAGAGG+AGG + chr2.4:73368805-73368824 MS.gene01230:CDS 35.0%
TATGGCAGTAAAATTTGGGA+AGG + chr2.4:73368751-73368770 MS.gene01230:CDS 35.0%
TCCTGAAAACAGATGAATGA+AGG - chr2.4:73369237-73369256 None:intergenic 35.0%
TTTATAATTGACACGGCATC+GGG + chr2.4:73369026-73369045 MS.gene01230:CDS 35.0%
TTTGGGCAAGGAATAAACAT+GGG + chr2.4:73368062-73368081 MS.gene01230:CDS 35.0%
! ATATATGGTCTTGCATATGC+AGG + chr2.4:73368579-73368598 MS.gene01230:intron 35.0%
! ATGACACTTCTGAAGATATC+TGG + chr2.4:73369071-73369090 MS.gene01230:CDS 35.0%
! CTGCAATAACCAGTAACTTT+TGG - chr2.4:73367829-73367848 None:intergenic 35.0%
! GAAGCTAAAGGATAGCTTTT+AGG - chr2.4:73368620-73368639 None:intergenic 35.0%
! TCCTTCATTCATCTGTTTTC+AGG + chr2.4:73369233-73369252 MS.gene01230:intron 35.0%
! TCTCATTCTGCTTTGTTGAA+TGG + chr2.4:73367555-73367574 MS.gene01230:intron 35.0%
! TGAAAGTGTAGCATCAAACT+AGG - chr2.4:73367854-73367873 None:intergenic 35.0%
! TGCATTTATGTATGACTTGC+TGG + chr2.4:73367911-73367930 MS.gene01230:intron 35.0%
! TTTAAAGTCACACACATGCT+TGG + chr2.4:73367697-73367716 MS.gene01230:intron 35.0%
!! ATCTTCAGAAGTGTCATGTA+AGG - chr2.4:73369068-73369087 None:intergenic 35.0%
!! GTAAAATTTGGGAAGGCATT+TGG + chr2.4:73368758-73368777 MS.gene01230:CDS 35.0%
AATCAACCTGGTAAGTCTCT+TGG + chr2.4:73368701-73368720 MS.gene01230:CDS 40.0%
ACCTTGCTACCAAAAGTTAC+TGG + chr2.4:73367817-73367836 MS.gene01230:intron 40.0%
ACTCAAATGAAGCTCTTGAG+AGG + chr2.4:73369369-73369388 MS.gene01230:CDS 40.0%
ATGCTGATGTAATTTGGGCA+AGG + chr2.4:73368050-73368069 MS.gene01230:CDS 40.0%
CAACTCGTTCTTTATATCGC+TGG + chr2.4:73369196-73369215 MS.gene01230:intron 40.0%
CAAGGTTGCCTTGAAACAAA+AGG - chr2.4:73367803-73367822 None:intergenic 40.0%
CTCAAATGAAGCTCTTGAGA+GGG + chr2.4:73369370-73369389 MS.gene01230:CDS 40.0%
GCTTCATTTGAGTACCCAAT+TGG - chr2.4:73369362-73369381 None:intergenic 40.0%
GTACTTACATCAACTCATGC+AGG + chr2.4:73368277-73368296 MS.gene01230:CDS 40.0%
GTGTCATGTAAGGATCAAAC+GGG - chr2.4:73369058-73369077 None:intergenic 40.0%
TACCTAAGTTAAGGCTTGCA+GGG - chr2.4:73369139-73369158 None:intergenic 40.0%
TCTGAAGATATCTGGTGTTC+TGG + chr2.4:73369079-73369098 MS.gene01230:CDS 40.0%
TCTTGAGAGGGTCGTAAATA+AGG + chr2.4:73369382-73369401 MS.gene01230:CDS 40.0%
TTACCTAAGTTAAGGCTTGC+AGG - chr2.4:73369140-73369159 None:intergenic 40.0%
TTATCAGACACGAGATTGCT+GGG + chr2.4:73368193-73368212 MS.gene01230:intron 40.0%
TTGGGCAAGGAATAAACATG+GGG + chr2.4:73368063-73368082 MS.gene01230:CDS 40.0%
! AGCTATTCTTGCTGTGTGTA+TGG + chr2.4:73368121-73368140 MS.gene01230:intron 40.0%
! ATTTTACTGCCATATGACCG+AGG - chr2.4:73368745-73368764 None:intergenic 40.0%
! GGCTGAATTTGACTTCACTT+GGG - chr2.4:73369118-73369137 None:intergenic 40.0%
! TTCAAAGTATCCTGTTGTGC+CGG + chr2.4:73368087-73368106 MS.gene01230:CDS 40.0%
!! ACCAGTAACTTTTGGTAGCA+AGG - chr2.4:73367821-73367840 None:intergenic 40.0%
AAAGGACCTGCTGAAGCTAA+AGG - chr2.4:73368632-73368651 None:intergenic 45.0%
AATGTCCAGCGTTTCAAGGT+TGG + chr2.4:73368237-73368256 MS.gene01230:CDS 45.0%
ACCTTGAAACGCTGGACATT+AGG - chr2.4:73368237-73368256 None:intergenic 45.0%
CGCCTTACAAATTCAATCGC+AGG + chr2.4:73367515-73367534 MS.gene01230:CDS 45.0%
CTTATCAGACACGAGATTGC+TGG + chr2.4:73368192-73368211 MS.gene01230:intron 45.0%
GAATAGCTCACAACTTACCC+CGG - chr2.4:73368109-73368128 None:intergenic 45.0%
GTGATTCCAGCACAAAGCAA+AGG - chr2.4:73368650-73368669 None:intergenic 45.0%
TATCAGACACGAGATTGCTG+GGG + chr2.4:73368194-73368213 MS.gene01230:intron 45.0%
TCAGGATCAAGAACTGTTGC+CGG + chr2.4:73369251-73369270 MS.gene01230:intron 45.0%
TCCTAATGTCCAGCGTTTCA+AGG + chr2.4:73368233-73368252 MS.gene01230:CDS 45.0%
TCGACTTCTGTGCTGCAAAT+GGG + chr2.4:73369312-73369331 MS.gene01230:CDS 45.0%
TGGTGAAGATTGCCTTGCAT+GGG + chr2.4:73367465-73367484 MS.gene01230:CDS 45.0%
TGTAAGTACCCACTCCAACA+TGG - chr2.4:73368266-73368285 None:intergenic 45.0%
TTGGTGAAGATTGCCTTGCA+TGG + chr2.4:73367464-73367483 MS.gene01230:CDS 45.0%
! AGCTATCCTTTAGCTTCAGC+AGG + chr2.4:73368623-73368642 MS.gene01230:CDS 45.0%
! CAAATTGGTCTGCACCAAGA+AGG - chr2.4:73368838-73368857 None:intergenic 45.0%
! GGGCTGAATTTGACTTCACT+TGG - chr2.4:73369119-73369138 None:intergenic 45.0%
! TCAAAGTATCCTGTTGTGCC+GGG + chr2.4:73368088-73368107 MS.gene01230:CDS 45.0%
!! CTGGTGTTCTGGTCTTAGTT+GGG + chr2.4:73369090-73369109 MS.gene01230:CDS 45.0%
!! GGCATTTGGTTTGCGTGTAA+CGG + chr2.4:73368772-73368791 MS.gene01230:CDS 45.0%
!! TCTGGTGTTCTGGTCTTAGT+TGG + chr2.4:73369089-73369108 MS.gene01230:CDS 45.0%
!! TTCAAGGTTGGTGACCATGT+TGG + chr2.4:73368249-73368268 MS.gene01230:CDS 45.0%
ACCACTCCAAGAGACTTACC+AGG - chr2.4:73368710-73368729 None:intergenic 50.0%
ACCTGGTAAGTCTCTTGGAG+TGG + chr2.4:73368706-73368725 MS.gene01230:CDS 50.0%
ACTGACGGTGACTTCTATGC+AGG + chr2.4:73368983-73369002 MS.gene01230:intron 50.0%
AGCCCTGCAAGCCTTAACTT+AGG + chr2.4:73369134-73369153 MS.gene01230:CDS 50.0%
GGTAAGTCTCTTGGAGTGGT+TGG + chr2.4:73368710-73368729 MS.gene01230:CDS 50.0%
TGGTGATTGCTAAGGGAGCT+CGG + chr2.4:73367983-73368002 MS.gene01230:intron 50.0%
TGTACCACCTGCAACACTTC+CGG - chr2.4:73369273-73369292 None:intergenic 50.0%
! CAAAGTATCCTGTTGTGCCG+GGG + chr2.4:73368089-73368108 MS.gene01230:CDS 50.0%
! GTCGACTTCTGTGCTGCAAA+TGG + chr2.4:73369311-73369330 MS.gene01230:CDS 50.0%
!! GCAGGCGTTGGCTAAATCAT+TGG + chr2.4:73369001-73369020 MS.gene01230:intron 50.0%
!! GTTGGTGACCATGTTGGAGT+GGG + chr2.4:73368255-73368274 MS.gene01230:CDS 50.0%
!! TCTCTTGGAGTGGTTGGTCT+TGG + chr2.4:73368716-73368735 MS.gene01230:CDS 50.0%
ACAACTTACCCCGGCACAAC+AGG - chr2.4:73368100-73368119 None:intergenic 55.0%
ACACGAGATTGCTGGGGTTG+TGG + chr2.4:73368200-73368219 MS.gene01230:CDS 55.0%
CATCTCTTGCTGCCCATGCA+AGG - chr2.4:73367480-73367499 None:intergenic 55.0%
GATTGCTGGGGTTGTGGCAA+AGG + chr2.4:73368206-73368225 MS.gene01230:CDS 55.0%
GCAGGTCCTTTGCTTTGTGC+TGG + chr2.4:73368641-73368660 MS.gene01230:CDS 55.0%
GGTCACCAACCTTGAAACGC+TGG - chr2.4:73368245-73368264 None:intergenic 55.0%
GGTGACTTCTATGCAGGCGT+TGG + chr2.4:73368989-73369008 MS.gene01230:intron 55.0%
GGTGATTGCTAAGGGAGCTC+GGG + chr2.4:73367984-73368003 MS.gene01230:intron 55.0%
TGGGCAGCAAGAGATGCATC+TGG + chr2.4:73367484-73367503 MS.gene01230:CDS 55.0%
! TCTTGGTGGCCTCGGTCATA+TGG + chr2.4:73368733-73368752 MS.gene01230:CDS 55.0%
!! ACTGTTGCCGGAAGTGTTGC+AGG + chr2.4:73369263-73369282 MS.gene01230:CDS 55.0%
!! CTTGGAGTGGTTGGTCTTGG+TGG + chr2.4:73368719-73368738 MS.gene01230:CDS 55.0%
!! GGTTGGTGACCATGTTGGAG+TGG + chr2.4:73368254-73368273 MS.gene01230:CDS 55.0%
GCTGGGGTTGTGGCAAAGGT+TGG + chr2.4:73368210-73368229 MS.gene01230:CDS 60.0%
!! GTGGTTGGTCTTGGTGGCCT+CGG + chr2.4:73368725-73368744 MS.gene01230:CDS 60.0%
!! GTTGCCGGAAGTGTTGCAGG+TGG + chr2.4:73369266-73369285 MS.gene01230:CDS 60.0%
!! GAGGAGGCGCTGAGCCTTCT+TGG + chr2.4:73368821-73368840 MS.gene01230:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 73367448 73369451 73367448 ID=MS.gene01230
chr2.4 mRNA 73367448 73369451 73367448 ID=MS.gene01230.t1;Parent=MS.gene01230
chr2.4 exon 73367448 73367536 73367448 ID=MS.gene01230.t1.exon1;Parent=MS.gene01230.t1
chr2.4 CDS 73367448 73367536 73367448 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
chr2.4 exon 73367997 73368110 73367997 ID=MS.gene01230.t1.exon2;Parent=MS.gene01230.t1
chr2.4 CDS 73367997 73368110 73367997 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
chr2.4 exon 73368200 73368430 73368200 ID=MS.gene01230.t1.exon3;Parent=MS.gene01230.t1
chr2.4 CDS 73368200 73368430 73368200 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
chr2.4 exon 73368601 73368886 73368601 ID=MS.gene01230.t1.exon4;Parent=MS.gene01230.t1
chr2.4 CDS 73368601 73368886 73368601 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
chr2.4 exon 73369005 73369155 73369005 ID=MS.gene01230.t1.exon5;Parent=MS.gene01230.t1
chr2.4 CDS 73369005 73369155 73369005 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
chr2.4 exon 73369255 73369451 73369255 ID=MS.gene01230.t1.exon6;Parent=MS.gene01230.t1
chr2.4 CDS 73369255 73369451 73369255 ID=cds.MS.gene01230.t1;Parent=MS.gene01230.t1
Gene Sequence

>MS.gene01230

ATGAGTTCTGAAAGTGTTGGTGAAGATTGCCTTGCATGGGCAGCAAGAGATGCATCTGGAGTTCTGTCGCCTTACAAATTCAATCGCAGGTATATTTGTTATGTACCTCTCATTCTGCTTTGTTGAATGGAGTAATTCTTTTAGGATTTAATTTTTATATATATACTGTCAGTGTAAAGGGTTTTTAGACTTGTCACTCAATCATAACCGTTAAGTCATTAGAAACATTTGACTTTTATCATAATCGCTTTTAAAGTCACACACATGCTTGGTAGTGATGTGTTGACAGTGTAAAACTTATACAATCAGTGTATGAAAGTTAAACTCCGAGTTTTAAGTAAAATCTCATTTTCCTTTTGTTTCAAGGCAACCTTGCTACCAAAAGTTACTGGTTATTGCAGTTCCTAGTTTGATGCTACACTTTCATATCATATCAATTCAAATGCCCACATATAAGAATGTATGCATTTATGTATGACTTGCTGGCCATGTTACATTTTTCCAAAAAAAAAATTAAATCCTGACAATATGATAATGGTGATTGCTAAGGGAGCTCGGGAGTGAAGATGTTTATGTTAAAATTACACACTGTGGCGTTTGTTATGCTGATGTAATTTGGGCAAGGAATAAACATGGGGATTCAAAGTATCCTGTTGTGCCGGGGTAAGTTGTGAGCTATTCTTGCTGTGTGTATGGTTGAGAATGTTATATACATAAGCCTACTTATCTGTGTTTTTCTTTTTTCTTATCAGACACGAGATTGCTGGGGTTGTGGCAAAGGTTGGTCCTAATGTCCAGCGTTTCAAGGTTGGTGACCATGTTGGAGTGGGTACTTACATCAACTCATGCAGGGAATGTGAGTATTGTAACGATCGATTAGAAGTTCATTGTGTGAAGGGATCAGTTTACACCTTTAATGGTGTTGATTATGATGGTACAATTACAAAAGGAGGATACTCTACTTCCATTGTAGTACATGAAAGGTAAACTTAATAGATGGCTTGGTTCTGATTATGTTTTATGTGTTTTTATGAGACCTTCTCTTGCTGAAATTTATTATAAGCACTAAAACAACTTCTTTGATTTCATAATAATGGAGGTTGTAATTTCATTCAGTTCATAACTTCTTTTATATATGGTCTTGCATATGCAGGTATTGCTTCTTGATACCTAAAAGCTATCCTTTAGCTTCAGCAGGTCCTTTGCTTTGTGCTGGAATCACTGTTTATTCTCCCATGATGCGACATAATATGAATCAACCTGGTAAGTCTCTTGGAGTGGTTGGTCTTGGTGGCCTCGGTCATATGGCAGTAAAATTTGGGAAGGCATTTGGTTTGCGTGTAACGGTTTTCAGCACTAGCATTTCAAAGAAAGAGGAGGCGCTGAGCCTTCTTGGTGCAGACCAATTTGTTGTTTCATCTAATCAAGAAGAGATGAGGGTAAGTATTATATGAACCATCTATACCATAGCATTTTCTTCAAACCATGAAGAGAATACTGATACTGATGACATGTGCTTCTGTTTTTTTAACTAAACTGACGGTGACTTCTATGCAGGCGTTGGCTAAATCATTGGATTTTATAATTGACACGGCATCGGGTGATCACCCGTTTGATCCTTACATGACACTTCTGAAGATATCTGGTGTTCTGGTCTTAGTTGGGTTCCCAAGTGAAGTCAAATTCAGCCCTGCAAGCCTTAACTTAGGTAATGTTGCATTTGAAGTATAGAAGTTTTGTTTGAATAACAACTCGTTCTTTATATCGCTGGAACCAGAAGTAATATCCTTCATTCATCTGTTTTCAGGATCAAGAACTGTTGCCGGAAGTGTTGCAGGTGGTACAAAAGAAATACAGGAGATGGTCGACTTCTGTGCTGCAAATGGGATTCACCCAGAAATAGAACTGATTCCAATTGGGTACTCAAATGAAGCTCTTGAGAGGGTCGTAAATAAGGACGTGAAATATCGGTTTGTAATAGACATTGAAAATTCCCTCAAATAG
Protein sequence

>MS.gene01230.t1

MSSESVGEDCLAWAARDASGVLSPYKFNRRELGSEDVYVKITHCGVCYADVIWARNKHGDSKYPVVPGHEIAGVVAKVGPNVQRFKVGDHVGVGTYINSCRECEYCNDRLEVHCVKGSVYTFNGVDYDGTITKGGYSTSIVVHERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSISKKEEALSLLGADQFVVSSNQEEMRALAKSLDFIIDTASGDHPFDPYMTLLKISGVLVLVGFPSEVKFSPASLNLGSRTVAGSVAGGTKEIQEMVDFCAANGIHPEIELIPIGYSNEALERVVNKDVKYRFVIDIENSLK