AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02214

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02214.t1 MTR_2g009810 97.183 355 10 0 1 355 52 406 0.0 716
MS.gene02214.t1 MTR_2g009820 89.113 248 23 2 112 355 7 254 1.70e-149 422
MS.gene02214.t1 MTR_5g031190 53.582 349 160 2 8 355 11 358 3.39e-124 362
MS.gene02214.t1 MTR_5g031360 54.155 349 158 2 8 355 13 360 1.34e-123 360
MS.gene02214.t1 MTR_5g031230 54.310 348 153 4 11 355 14 358 7.52e-120 350
MS.gene02214.t1 MTR_5g031310 53.314 347 158 3 11 355 15 359 7.02e-119 348
MS.gene02214.t1 MTR_5g031305 53.602 347 157 3 11 355 15 359 2.04e-118 347
MS.gene02214.t1 MTR_5g031390 52.450 347 161 3 11 355 14 358 3.92e-118 346
MS.gene02214.t1 MTR_5g031210 54.335 346 156 2 11 355 14 358 6.47e-118 345
MS.gene02214.t1 MTR_5g031250 52.312 346 163 2 11 355 14 358 5.07e-117 343
MS.gene02214.t1 MTR_4g115300 53.026 347 159 3 11 355 14 358 5.91e-117 343
MS.gene02214.t1 MTR_5g031180 54.913 346 154 2 11 355 14 358 1.76e-116 342
MS.gene02214.t1 MTR_5g031300 51.576 349 167 2 8 355 12 359 6.01e-116 340
MS.gene02214.t1 MTR_8g068190 54.335 346 156 2 11 355 14 358 5.98e-115 338
MS.gene02214.t1 MTR_8g092630 55.362 345 151 3 11 354 71 413 7.78e-114 337
MS.gene02214.t1 MTR_5g031430 51.437 348 163 3 11 355 14 358 1.58e-111 329
MS.gene02214.t1 MTR_1g025950 52.478 343 160 3 13 353 15 356 7.71e-110 325
MS.gene02214.t1 MTR_1g107425 45.070 355 192 3 1 353 1 354 7.15e-101 302
MS.gene02214.t1 MTR_1g107425 45.791 297 158 3 1 295 1 296 2.16e-80 248
MS.gene02214.t1 MTR_5g031250 51.653 242 112 2 11 248 14 254 1.19e-74 231
MS.gene02214.t1 MTR_5g031290 55.357 112 49 1 245 355 80 191 1.09e-30 115
MS.gene02214.t1 MTR_5g031290 52.857 70 32 1 8 76 11 80 7.74e-18 80.9
MS.gene02214.t1 MTR_8g095220 24.062 320 209 8 16 305 60 375 3.06e-17 82.8
MS.gene02214.t1 MTR_8g095220 25.911 247 161 5 16 242 60 304 3.44e-16 78.6
MS.gene02214.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.05e-13 71.2
MS.gene02214.t1 MTR_3g089940 23.919 347 229 10 36 351 37 379 2.35e-13 70.9
MS.gene02214.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.70e-13 70.1
MS.gene02214.t1 MTR_3g089940 24.573 293 195 7 36 304 37 327 3.87e-13 70.1
MS.gene02214.t1 MTR_0001s0660 26.280 293 190 9 34 304 34 322 3.96e-13 70.5
MS.gene02214.t1 MTR_3g089977 23.973 292 198 7 36 304 37 327 7.61e-13 69.3
MS.gene02214.t1 MTR_3g089970 23.698 384 256 11 1 351 1 380 8.00e-13 69.3
MS.gene02214.t1 MTR_8g064610 30.102 196 107 5 57 251 128 294 9.22e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02214.t1 AT1G72680 75.706 354 85 1 2 355 3 355 0.0 555
MS.gene02214.t1 AT4G37970 50.144 347 171 2 9 354 13 358 9.23e-114 335
MS.gene02214.t1 AT2G21890 52.874 348 157 4 11 354 8 352 1.63e-113 335
MS.gene02214.t1 AT4G39330 51.884 345 164 2 11 354 14 357 2.86e-113 334
MS.gene02214.t1 AT2G21730 52.738 347 160 4 11 354 8 353 2.67e-112 332
MS.gene02214.t1 AT4G37980 49.284 349 175 2 8 355 7 354 5.94e-104 310
MS.gene02214.t1 AT4G37990 48.138 349 179 2 8 355 7 354 1.40e-98 296
MS.gene02214.t1 AT3G19450 45.070 355 192 3 1 353 1 354 1.83e-91 278
MS.gene02214.t1 AT4G39330 51.034 290 140 2 11 299 14 302 1.79e-89 271
MS.gene02214.t1 AT4G34230 45.906 342 183 2 13 353 13 353 2.08e-88 270
MS.gene02214.t1 AT4G34230 45.906 342 183 2 13 353 13 353 2.70e-88 270
MS.gene02214.t1 AT4G37970 49.291 282 141 2 9 290 13 292 9.24e-87 264
MS.gene02214.t1 AT4G37980 49.817 273 136 1 8 280 7 278 1.18e-79 246
MS.gene02214.t1 AT4G37970 51.293 232 112 1 9 240 13 243 3.44e-75 233
MS.gene02214.t1 AT4G37970 51.293 232 112 1 9 240 13 243 3.87e-75 233
MS.gene02214.t1 AT5G63620 26.506 249 157 6 16 242 62 306 6.30e-17 80.9
MS.gene02214.t1 AT5G63620 25.225 333 215 9 16 318 62 390 8.35e-17 81.6
MS.gene02214.t1 AT5G63620 25.225 333 215 9 16 318 62 390 8.91e-17 81.6
MS.gene02214.t1 AT5G43940 26.222 225 135 4 36 238 36 251 9.33e-14 72.0
MS.gene02214.t1 AT5G43940 26.222 225 135 4 36 238 48 263 9.41e-14 72.4
MS.gene02214.t1 AT1G32780 25.552 317 188 10 36 312 37 345 3.44e-13 70.5
MS.gene02214.t1 AT1G77120 25.589 297 187 9 36 304 36 326 3.79e-12 67.4

Find 105 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCATATGGCAGTAAAATT+TGG 0.150937 2.1:+73341211 MS.gene02214:CDS
ATTGGAATCAGTTCTATTTC+TGG 0.200827 2.1:-73341806 None:intergenic
ATCATAATCAACACCATTAA+AGG 0.217231 2.1:-73340827 None:intergenic
GGATCAGTTTACACCTTTAA+TGG 0.243928 2.1:+73340814 MS.gene02214:CDS
TTTGTTATGCTGATGTAATT+TGG 0.289646 2.1:+73340513 MS.gene02214:CDS
GAGGAGGCGCTGAGCCTTCT+TGG 0.291889 2.1:+73341286 MS.gene02214:CDS
TCTGGTGTTCTGGTCTTAGT+TGG 0.292846 2.1:+73341554 MS.gene02214:CDS
TTACCTAAGTTAAGGCTTGC+AGG 0.313929 2.1:-73341602 None:intergenic
GTAAAATTTGGGAAGGCATT+TGG 0.314204 2.1:+73341223 MS.gene02214:CDS
TCTTGAGAGGGTCGTAAATA+AGG 0.324188 2.1:+73341847 MS.gene02214:CDS
TCTGAAGATATCTGGTGTTC+TGG 0.326227 2.1:+73341544 MS.gene02214:CDS
TTGTTATGCTGATGTAATTT+GGG 0.354641 2.1:+73340514 MS.gene02214:CDS
TGTACCACCTGCAACACTTC+CGG 0.359100 2.1:-73341735 None:intergenic
ACCTTGAAACGCTGGACATT+AGG 0.363094 2.1:-73340703 None:intergenic
CTTATCAGACACGAGATTGC+TGG 0.363214 2.1:+73340661 MS.gene02214:intron
GTCATATGGCAGTAAAATTT+GGG 0.370755 2.1:+73341212 MS.gene02214:CDS
TTGGAATCAGTTCTATTTCT+GGG 0.372653 2.1:-73341805 None:intergenic
GCAGGTCCTTTGCTTTGTGC+TGG 0.376092 2.1:+73341106 MS.gene02214:CDS
GATTAGATGAAACAACAAAT+TGG 0.376372 2.1:-73341315 None:intergenic
GAAATAGAACTGATTCCAAT+TGG 0.379523 2.1:+73341809 MS.gene02214:CDS
TCTCTTGGAGTGGTTGGTCT+TGG 0.380973 2.1:+73341181 MS.gene02214:CDS
CTGGTGTTCTGGTCTTAGTT+GGG 0.395199 2.1:+73341555 MS.gene02214:CDS
GTCGACTTCTGTGCTGCAAA+TGG 0.396290 2.1:+73341776 MS.gene02214:CDS
CTTGGAGTGGTTGGTCTTGG+TGG 0.399951 2.1:+73341184 MS.gene02214:CDS
TCTTGGTGGCCTCGGTCATA+TGG 0.406264 2.1:+73341198 MS.gene02214:CDS
GCAGGCGTTGGCTAAATCAT+TGG 0.409444 2.1:+73341466 MS.gene02214:intron
ATGACACTTCTGAAGATATC+TGG 0.409523 2.1:+73341536 MS.gene02214:CDS
ATGCAACATTACCTAAGTTA+AGG 0.414851 2.1:-73341610 None:intergenic
TTTATAATTGACACGGCATC+GGG 0.416518 2.1:+73341491 MS.gene02214:CDS
TCAGGATCAAGAACTGTTGC+CGG 0.416934 2.1:+73341716 MS.gene02214:intron
GCTTCATTTGAGTACCCAAT+TGG 0.417911 2.1:-73341824 None:intergenic
TAAATAAGGACGTGAAATAT+CGG 0.419184 2.1:+73341861 MS.gene02214:CDS
TACCTGCGATTGAATTTGTA+AGG 0.426854 2.1:-73339984 None:intergenic
GGGCTGAATTTGACTTCACT+TGG 0.427549 2.1:-73341581 None:intergenic
ACTGTTGCCGGAAGTGTTGC+AGG 0.432996 2.1:+73341728 MS.gene02214:CDS
ACAACTTACCCCGGCACAAC+AGG 0.440053 2.1:-73340566 None:intergenic
TCCTAATGTCCAGCGTTTCA+AGG 0.445977 2.1:+73340702 MS.gene02214:CDS
GTGGTTGGTCTTGGTGGCCT+CGG 0.451789 2.1:+73341190 MS.gene02214:CDS
TTCAAAGTATCCTGTTGTGC+CGG 0.454354 2.1:+73340556 MS.gene02214:CDS
AATCAACCTGGTAAGTCTCT+TGG 0.459390 2.1:+73341166 MS.gene02214:CDS
TCAAAGTATCCTGTTGTGCC+GGG 0.460698 2.1:+73340557 MS.gene02214:CDS
AGCTATCCTTTAGCTTCAGC+AGG 0.468468 2.1:+73341088 MS.gene02214:CDS
ACACGAGATTGCTGGGGTTG+TGG 0.469150 2.1:+73340669 MS.gene02214:CDS
GCTGGGGTTGTGGCAAAGGT+TGG 0.470558 2.1:+73340679 MS.gene02214:CDS
ATTCATATTATGTCGCATCA+TGG 0.471650 2.1:-73341146 None:intergenic
TTATCAGACACGAGATTGCT+GGG 0.475272 2.1:+73340662 MS.gene02214:intron
GGCATTTGGTTTGCGTGTAA+CGG 0.491251 2.1:+73341237 MS.gene02214:CDS
CGCCTTACAAATTCAATCGC+AGG 0.493938 2.1:+73339982 MS.gene02214:CDS
ATCTTCAGAAGTGTCATGTA+AGG 0.496050 2.1:-73341530 None:intergenic
AGGTGGTACAAAAGAAATAC+AGG 0.497345 2.1:+73341748 MS.gene02214:CDS
GGTAAGTCTCTTGGAGTGGT+TGG 0.503337 2.1:+73341175 MS.gene02214:CDS
TGGTGATTGCTAAGGGAGCT+CGG 0.505418 2.1:+73340452 MS.gene02214:intron
TATGATGGTACAATTACAAA+AGG 0.507275 2.1:+73340844 MS.gene02214:CDS
TGGGCAGCAAGAGATGCATC+TGG 0.511542 2.1:+73339951 MS.gene02214:CDS
TTTAATGGTGTTGATTATGA+TGG 0.517784 2.1:+73340829 MS.gene02214:CDS
TCGACTTCTGTGCTGCAAAT+GGG 0.518039 2.1:+73341777 MS.gene02214:CDS
GGTGATTGCTAAGGGAGCTC+GGG 0.518767 2.1:+73340453 MS.gene02214:intron
CATCTCTTGCTGCCCATGCA+AGG 0.525887 2.1:-73339944 None:intergenic
TTGGTGAAGATTGCCTTGCA+TGG 0.528700 2.1:+73339931 MS.gene02214:CDS
TTCAAGGTTGGTGACCATGT+TGG 0.531246 2.1:+73340718 MS.gene02214:CDS
AGTGTCATGTAAGGATCAAA+CGG 0.531817 2.1:-73341521 None:intergenic
TTCATGTACTACAATGGAAG+TGG 0.531892 2.1:-73340875 None:intergenic
CACTAGCATTTCAAAGAAAG+AGG 0.533511 2.1:+73341267 MS.gene02214:CDS
AAATAGAACTGATTCCAATT+GGG 0.533719 2.1:+73341810 MS.gene02214:CDS
AAAGGACCTGCTGAAGCTAA+AGG 0.536529 2.1:-73341094 None:intergenic
AATGTCCAGCGTTTCAAGGT+TGG 0.539954 2.1:+73340706 MS.gene02214:CDS
GATTGCTGGGGTTGTGGCAA+AGG 0.541441 2.1:+73340675 MS.gene02214:CDS
GGCTGAATTTGACTTCACTT+GGG 0.543028 2.1:-73341580 None:intergenic
TTCATATTATGTCGCATCAT+GGG 0.544385 2.1:-73341145 None:intergenic
TACCTAAGTTAAGGCTTGCA+GGG 0.544423 2.1:-73341601 None:intergenic
TGGTGAAGATTGCCTTGCAT+GGG 0.548223 2.1:+73339932 MS.gene02214:CDS
TACAAAAGAAATACAGGAGA+TGG 0.550321 2.1:+73341754 MS.gene02214:CDS
ATTAGAAGTTCATTGTGTGA+AGG 0.552235 2.1:+73340792 MS.gene02214:CDS
TTAGAAGTTCATTGTGTGAA+GGG 0.558615 2.1:+73340793 MS.gene02214:CDS
GTACTTACATCAACTCATGC+AGG 0.564363 2.1:+73340746 MS.gene02214:CDS
GTGTCATGTAAGGATCAAAC+GGG 0.564881 2.1:-73341520 None:intergenic
CGACATAATATGAATCAACC+TGG 0.568407 2.1:+73341154 MS.gene02214:CDS
AGCCCTGCAAGCCTTAACTT+AGG 0.570562 2.1:+73341599 MS.gene02214:CDS
TTACCTTTCATGTACTACAA+TGG 0.576358 2.1:-73340881 None:intergenic
CATCTAATCAAGAAGAGATG+AGG 0.579627 2.1:+73341329 MS.gene02214:CDS
CAAATTGGTCTGCACCAAGA+AGG 0.580444 2.1:-73341300 None:intergenic
CTTCCATTGTAGTACATGAA+AGG 0.582389 2.1:+73340878 MS.gene02214:CDS
GTGATTCCAGCACAAAGCAA+AGG 0.590358 2.1:-73341112 None:intergenic
GGTCACCAACCTTGAAACGC+TGG 0.591766 2.1:-73340711 None:intergenic
ATCTAATCAAGAAGAGATGA+GGG 0.596139 2.1:+73341330 MS.gene02214:CDS
ACTCAAATGAAGCTCTTGAG+AGG 0.606095 2.1:+73341834 MS.gene02214:CDS
TTTGGGCAAGGAATAAACAT+GGG 0.615486 2.1:+73340531 MS.gene02214:CDS
ACCTGGTAAGTCTCTTGGAG+TGG 0.618165 2.1:+73341171 MS.gene02214:CDS
TATGGCAGTAAAATTTGGGA+AGG 0.622661 2.1:+73341216 MS.gene02214:CDS
CTCAAATGAAGCTCTTGAGA+GGG 0.622942 2.1:+73341835 MS.gene02214:CDS
GATGGTACAATTACAAAAGG+AGG 0.626222 2.1:+73340847 MS.gene02214:CDS
ATTTGGGCAAGGAATAAACA+TGG 0.627500 2.1:+73340530 MS.gene02214:CDS
ATGCTGATGTAATTTGGGCA+AGG 0.629018 2.1:+73340519 MS.gene02214:CDS
TACTTACATCAACTCATGCA+GGG 0.634667 2.1:+73340747 MS.gene02214:CDS
CAAAGTATCCTGTTGTGCCG+GGG 0.639115 2.1:+73340558 MS.gene02214:CDS
GGTTGGTGACCATGTTGGAG+TGG 0.639841 2.1:+73340723 MS.gene02214:CDS
TTGGGCAAGGAATAAACATG+GGG 0.641841 2.1:+73340532 MS.gene02214:CDS
TATGTTAAAATTACACACTG+TGG 0.642017 2.1:+73340488 MS.gene02214:CDS
GTTGGTGACCATGTTGGAGT+GGG 0.658549 2.1:+73340724 MS.gene02214:CDS
TGTAAGTACCCACTCCAACA+TGG 0.660381 2.1:-73340732 None:intergenic
ACCACTCCAAGAGACTTACC+AGG 0.671497 2.1:-73341172 None:intergenic
TAGCATTTCAAAGAAAGAGG+AGG 0.681888 2.1:+73341270 MS.gene02214:CDS
TATCAGACACGAGATTGCTG+GGG 0.696618 2.1:+73340663 MS.gene02214:intron
GCTATGAGTTCTGAAAGTGT+TGG 0.742932 2.1:+73339912 None:intergenic
GTTGCCGGAAGTGTTGCAGG+TGG 0.789484 2.1:+73341731 MS.gene02214:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATAACTTCTTTTATATA+TGG + chr2.1:73341029-73341048 MS.gene02214:intron 15.0%
!!! AAATGTTTCTAATGACTTAA+CGG - chr2.1:73340125-73340144 None:intergenic 20.0%
!!! ATGAGATTTTACTTAAAACT+CGG - chr2.1:73340248-73340267 None:intergenic 20.0%
!!! ATTGGATTTTATAATTGACA+CGG + chr2.1:73341484-73341503 MS.gene02214:CDS 20.0%
!!! CTTCTTTGATTTCATAATAA+TGG + chr2.1:73340991-73341010 MS.gene02214:intron 20.0%
!!! TGTCAGGATTTAATTTTTTT+TGG - chr2.1:73340423-73340442 None:intergenic 20.0%
!!! TGTTTTTTTAACTAAACTGA+TGG + chr2.1:73341433-73341452 MS.gene02214:intron 20.0%
!!! TTTTTTGGAAAAATGTAACA+TGG - chr2.1:73340408-73340427 None:intergenic 20.0%
! AAAGAAAAACACAGATAAGT+AGG - chr2.1:73340638-73340657 None:intergenic 25.0%
! AAATCCTGACAATATGATAA+TGG + chr2.1:73340432-73340451 MS.gene02214:intron 25.0%
! AATGCATACATTCTTATATG+TGG - chr2.1:73340368-73340387 None:intergenic 25.0%
! ATATATACTGTCAGTGTAAA+GGG + chr2.1:73340073-73340092 MS.gene02214:intron 25.0%
! ATCATAATCAACACCATTAA+AGG - chr2.1:73340830-73340849 None:intergenic 25.0%
! ATGCATACATTCTTATATGT+GGG - chr2.1:73340367-73340386 None:intergenic 25.0%
! GATTAGATGAAACAACAAAT+TGG - chr2.1:73341318-73341337 None:intergenic 25.0%
! TAAATAAGGACGTGAAATAT+CGG + chr2.1:73341861-73341880 MS.gene02214:CDS 25.0%
! TAATAAATTTCAGCAAGAGA+AGG - chr2.1:73340956-73340975 None:intergenic 25.0%
! TATATATACTGTCAGTGTAA+AGG + chr2.1:73340072-73340091 MS.gene02214:intron 25.0%
! TATGATGGTACAATTACAAA+AGG + chr2.1:73340844-73340863 MS.gene02214:CDS 25.0%
! TATGTTAAAATTACACACTG+TGG + chr2.1:73340488-73340507 MS.gene02214:CDS 25.0%
! TGAAAGGTAAACTTAATAGA+TGG + chr2.1:73340894-73340913 MS.gene02214:intron 25.0%
! TTGTTATGCTGATGTAATTT+GGG + chr2.1:73340514-73340533 MS.gene02214:CDS 25.0%
!! AAATAGAACTGATTCCAATT+GGG + chr2.1:73341810-73341829 MS.gene02214:CDS 25.0%
!! CTTTGATTTCATAATAATGG+AGG + chr2.1:73340994-73341013 MS.gene02214:intron 25.0%
!! TAGAATGGAGTAATTCTTTT+AGG + chr2.1:73340037-73340056 MS.gene02214:intron 25.0%
!! TTTGTTATGCTGATGTAATT+TGG + chr2.1:73340513-73340532 MS.gene02214:CDS 25.0%
!!! TTTAATGGTGTTGATTATGA+TGG + chr2.1:73340829-73340848 MS.gene02214:CDS 25.0%
AATTACTCCATTCTACAAAG+CGG - chr2.1:73340032-73340051 None:intergenic 30.0%
AGAAAATGCTATGGTATAGA+TGG - chr2.1:73341371-73341390 None:intergenic 30.0%
ATCACCATTATCATATTGTC+AGG - chr2.1:73340439-73340458 None:intergenic 30.0%
ATCTAATCAAGAAGAGATGA+GGG + chr2.1:73341330-73341349 MS.gene02214:CDS 30.0%
ATGCAACATTACCTAAGTTA+AGG - chr2.1:73341613-73341632 None:intergenic 30.0%
ATGGTTTGAAGAAAATGCTA+TGG - chr2.1:73341380-73341399 None:intergenic 30.0%
ATTAGAAGTTCATTGTGTGA+AGG + chr2.1:73340792-73340811 MS.gene02214:CDS 30.0%
ATTCATATTATGTCGCATCA+TGG - chr2.1:73341149-73341168 None:intergenic 30.0%
ATTGGAATCAGTTCTATTTC+TGG - chr2.1:73341809-73341828 None:intergenic 30.0%
GTCATATGGCAGTAAAATTT+GGG + chr2.1:73341212-73341231 MS.gene02214:CDS 30.0%
TACAAAAGAAATACAGGAGA+TGG + chr2.1:73341754-73341773 MS.gene02214:CDS 30.0%
TGAATGAAGGATATTACTTC+TGG - chr2.1:73341689-73341708 None:intergenic 30.0%
TTACCTTTCATGTACTACAA+TGG - chr2.1:73340884-73340903 None:intergenic 30.0%
TTAGAAGTTCATTGTGTGAA+GGG + chr2.1:73340793-73340812 MS.gene02214:CDS 30.0%
TTCATATTATGTCGCATCAT+GGG - chr2.1:73341148-73341167 None:intergenic 30.0%
! GAAATAGAACTGATTCCAAT+TGG + chr2.1:73341809-73341828 MS.gene02214:CDS 30.0%
! TTGGAATCAGTTCTATTTCT+GGG - chr2.1:73341808-73341827 None:intergenic 30.0%
! TTTTATAATTGACACGGCAT+CGG + chr2.1:73341490-73341509 MS.gene02214:CDS 30.0%
!! ATGATAATGGTGATTGCTAA+GGG + chr2.1:73340445-73340464 MS.gene02214:intron 30.0%
!! CTCATTTTCCTTTTGTTTCA+AGG + chr2.1:73340263-73340282 MS.gene02214:intron 30.0%
!! TATGATAATGGTGATTGCTA+AGG + chr2.1:73340444-73340463 MS.gene02214:intron 30.0%
AGGTGGTACAAAAGAAATAC+AGG + chr2.1:73341748-73341767 MS.gene02214:CDS 35.0%
AGTGTCATGTAAGGATCAAA+CGG - chr2.1:73341524-73341543 None:intergenic 35.0%
ATTTGGGCAAGGAATAAACA+TGG + chr2.1:73340530-73340549 MS.gene02214:CDS 35.0%
CACTAGCATTTCAAAGAAAG+AGG + chr2.1:73341267-73341286 MS.gene02214:CDS 35.0%
CAGTATCAGTATTCTCTTCA+TGG - chr2.1:73341399-73341418 None:intergenic 35.0%
CATCTAATCAAGAAGAGATG+AGG + chr2.1:73341329-73341348 MS.gene02214:CDS 35.0%
CATGAAGAGAATACTGATAC+TGG + chr2.1:73341397-73341416 MS.gene02214:intron 35.0%
CGACATAATATGAATCAACC+TGG + chr2.1:73341154-73341173 MS.gene02214:CDS 35.0%
CTTCCATTGTAGTACATGAA+AGG + chr2.1:73340878-73340897 MS.gene02214:CDS 35.0%
GATGGTACAATTACAAAAGG+AGG + chr2.1:73340847-73340866 MS.gene02214:CDS 35.0%
GGATCAGTTTACACCTTTAA+TGG + chr2.1:73340814-73340833 MS.gene02214:CDS 35.0%
GGTAAACTTAATAGATGGCT+TGG + chr2.1:73340899-73340918 MS.gene02214:intron 35.0%
GGTCATATGGCAGTAAAATT+TGG + chr2.1:73341211-73341230 MS.gene02214:CDS 35.0%
TACCTGCGATTGAATTTGTA+AGG - chr2.1:73339987-73340006 None:intergenic 35.0%
TACTTACATCAACTCATGCA+GGG + chr2.1:73340747-73340766 MS.gene02214:CDS 35.0%
TAGCATTTCAAAGAAAGAGG+AGG + chr2.1:73341270-73341289 MS.gene02214:CDS 35.0%
TATGGCAGTAAAATTTGGGA+AGG + chr2.1:73341216-73341235 MS.gene02214:CDS 35.0%
TCCTGAAAACAGATGAATGA+AGG - chr2.1:73341702-73341721 None:intergenic 35.0%
TTCATGTACTACAATGGAAG+TGG - chr2.1:73340878-73340897 None:intergenic 35.0%
TTTATAATTGACACGGCATC+GGG + chr2.1:73341491-73341510 MS.gene02214:CDS 35.0%
TTTGGGCAAGGAATAAACAT+GGG + chr2.1:73340531-73340550 MS.gene02214:CDS 35.0%
! AGCTATTCTTGTTGTGTGTA+TGG + chr2.1:73340590-73340609 MS.gene02214:intron 35.0%
! ATATATGGTCTTGCATATGC+AGG + chr2.1:73341044-73341063 MS.gene02214:intron 35.0%
! ATGACACTTCTGAAGATATC+TGG + chr2.1:73341536-73341555 MS.gene02214:CDS 35.0%
! CTGCAATAACCAGTAACTTT+TGG - chr2.1:73340300-73340319 None:intergenic 35.0%
! GAAGCTAAAGGATAGCTTTT+AGG - chr2.1:73341085-73341104 None:intergenic 35.0%
! TCCTTCATTCATCTGTTTTC+AGG + chr2.1:73341698-73341717 MS.gene02214:intron 35.0%
! TGAAAGTGTAGCATCAAACT+AGG - chr2.1:73340325-73340344 None:intergenic 35.0%
! TGCATTTATGTATGACTTGC+TGG + chr2.1:73340382-73340401 MS.gene02214:intron 35.0%
!! ATCTTCAGAAGTGTCATGTA+AGG - chr2.1:73341533-73341552 None:intergenic 35.0%
!! GTAAAATTTGGGAAGGCATT+TGG + chr2.1:73341223-73341242 MS.gene02214:CDS 35.0%
AATCAACCTGGTAAGTCTCT+TGG + chr2.1:73341166-73341185 MS.gene02214:CDS 40.0%
ACCTTGCTACCAAAAGTTAC+TGG + chr2.1:73340288-73340307 MS.gene02214:intron 40.0%
ACTCAAATGAAGCTCTTGAG+AGG + chr2.1:73341834-73341853 MS.gene02214:CDS 40.0%
ATGCTGATGTAATTTGGGCA+AGG + chr2.1:73340519-73340538 MS.gene02214:CDS 40.0%
ATTCTACAAAGCGGAATGTG+AGG - chr2.1:73340023-73340042 None:intergenic 40.0%
CAACTCGTTCTTTATATCGC+TGG + chr2.1:73341661-73341680 MS.gene02214:intron 40.0%
CAAGGTTGCCTTGAAACAAA+AGG - chr2.1:73340274-73340293 None:intergenic 40.0%
CTCAAATGAAGCTCTTGAGA+GGG + chr2.1:73341835-73341854 MS.gene02214:CDS 40.0%
GCTTCATTTGAGTACCCAAT+TGG - chr2.1:73341827-73341846 None:intergenic 40.0%
GTACTTACATCAACTCATGC+AGG + chr2.1:73340746-73340765 MS.gene02214:CDS 40.0%
GTGTCATGTAAGGATCAAAC+GGG - chr2.1:73341523-73341542 None:intergenic 40.0%
TACCTAAGTTAAGGCTTGCA+GGG - chr2.1:73341604-73341623 None:intergenic 40.0%
TCACATTCCGCTTTGTAGAA+TGG + chr2.1:73340022-73340041 MS.gene02214:intron 40.0%
TCTGAAGATATCTGGTGTTC+TGG + chr2.1:73341544-73341563 MS.gene02214:CDS 40.0%
TCTTGAGAGGGTCGTAAATA+AGG + chr2.1:73341847-73341866 MS.gene02214:CDS 40.0%
TTACCTAAGTTAAGGCTTGC+AGG - chr2.1:73341605-73341624 None:intergenic 40.0%
TTATCAGACACGAGATTGCT+GGG + chr2.1:73340662-73340681 MS.gene02214:intron 40.0%
TTGGGCAAGGAATAAACATG+GGG + chr2.1:73340532-73340551 MS.gene02214:CDS 40.0%
! ATTTTACTGCCATATGACCG+AGG - chr2.1:73341210-73341229 None:intergenic 40.0%
! GGCTGAATTTGACTTCACTT+GGG - chr2.1:73341583-73341602 None:intergenic 40.0%
! TCTAAAGTCACACACATGCT+TGG + chr2.1:73340164-73340183 MS.gene02214:intron 40.0%
! TTCAAAGTATCCTGTTGTGC+CGG + chr2.1:73340556-73340575 MS.gene02214:CDS 40.0%
!! ACCAGTAACTTTTGGTAGCA+AGG - chr2.1:73340292-73340311 None:intergenic 40.0%
AAAGGACCTGCTGAAGCTAA+AGG - chr2.1:73341097-73341116 None:intergenic 45.0%
AATGTCCAGCGTTTCAAGGT+TGG + chr2.1:73340706-73340725 MS.gene02214:CDS 45.0%
ACCTTGAAACGCTGGACATT+AGG - chr2.1:73340706-73340725 None:intergenic 45.0%
CGCCTTACAAATTCAATCGC+AGG + chr2.1:73339982-73340001 MS.gene02214:CDS 45.0%
CTTATCAGACACGAGATTGC+TGG + chr2.1:73340661-73340680 MS.gene02214:intron 45.0%
GAATAGCTCACAACTTACCC+CGG - chr2.1:73340578-73340597 None:intergenic 45.0%
GTGATTCCAGCACAAAGCAA+AGG - chr2.1:73341115-73341134 None:intergenic 45.0%
TATCAGACACGAGATTGCTG+GGG + chr2.1:73340663-73340682 MS.gene02214:intron 45.0%
TCAGGATCAAGAACTGTTGC+CGG + chr2.1:73341716-73341735 MS.gene02214:intron 45.0%
TCCTAATGTCCAGCGTTTCA+AGG + chr2.1:73340702-73340721 MS.gene02214:CDS 45.0%
TCGACTTCTGTGCTGCAAAT+GGG + chr2.1:73341777-73341796 MS.gene02214:CDS 45.0%
TGGTGAAGATTGCCTTGCAT+GGG + chr2.1:73339932-73339951 MS.gene02214:CDS 45.0%
TGTAAGTACCCACTCCAACA+TGG - chr2.1:73340735-73340754 None:intergenic 45.0%
TTGGTGAAGATTGCCTTGCA+TGG + chr2.1:73339931-73339950 MS.gene02214:CDS 45.0%
! ACTGATGGTGACTTCTATGC+AGG + chr2.1:73341448-73341467 MS.gene02214:intron 45.0%
! AGCTATCCTTTAGCTTCAGC+AGG + chr2.1:73341088-73341107 MS.gene02214:CDS 45.0%
! CAAATTGGTCTGCACCAAGA+AGG - chr2.1:73341303-73341322 None:intergenic 45.0%
! GGGCTGAATTTGACTTCACT+TGG - chr2.1:73341584-73341603 None:intergenic 45.0%
! TCAAAGTATCCTGTTGTGCC+GGG + chr2.1:73340557-73340576 MS.gene02214:CDS 45.0%
!! CTGGTGTTCTGGTCTTAGTT+GGG + chr2.1:73341555-73341574 MS.gene02214:CDS 45.0%
!! GGCATTTGGTTTGCGTGTAA+CGG + chr2.1:73341237-73341256 MS.gene02214:CDS 45.0%
!! TCTGGTGTTCTGGTCTTAGT+TGG + chr2.1:73341554-73341573 MS.gene02214:CDS 45.0%
!! TTCAAGGTTGGTGACCATGT+TGG + chr2.1:73340718-73340737 MS.gene02214:CDS 45.0%
ACCACTCCAAGAGACTTACC+AGG - chr2.1:73341175-73341194 None:intergenic 50.0%
ACCTGGTAAGTCTCTTGGAG+TGG + chr2.1:73341171-73341190 MS.gene02214:CDS 50.0%
AGCCCTGCAAGCCTTAACTT+AGG + chr2.1:73341599-73341618 MS.gene02214:CDS 50.0%
GGTAAGTCTCTTGGAGTGGT+TGG + chr2.1:73341175-73341194 MS.gene02214:CDS 50.0%
TGGTGATTGCTAAGGGAGCT+CGG + chr2.1:73340452-73340471 MS.gene02214:intron 50.0%
TGTACCACCTGCAACACTTC+CGG - chr2.1:73341738-73341757 None:intergenic 50.0%
! CAAAGTATCCTGTTGTGCCG+GGG + chr2.1:73340558-73340577 MS.gene02214:CDS 50.0%
! GTCGACTTCTGTGCTGCAAA+TGG + chr2.1:73341776-73341795 MS.gene02214:CDS 50.0%
!! GCAGGCGTTGGCTAAATCAT+TGG + chr2.1:73341466-73341485 MS.gene02214:intron 50.0%
!! GTTGGTGACCATGTTGGAGT+GGG + chr2.1:73340724-73340743 MS.gene02214:CDS 50.0%
!! TCTCTTGGAGTGGTTGGTCT+TGG + chr2.1:73341181-73341200 MS.gene02214:CDS 50.0%
ACAACTTACCCCGGCACAAC+AGG - chr2.1:73340569-73340588 None:intergenic 55.0%
ACACGAGATTGCTGGGGTTG+TGG + chr2.1:73340669-73340688 MS.gene02214:CDS 55.0%
CATCTCTTGCTGCCCATGCA+AGG - chr2.1:73339947-73339966 None:intergenic 55.0%
GATTGCTGGGGTTGTGGCAA+AGG + chr2.1:73340675-73340694 MS.gene02214:CDS 55.0%
GCAGGTCCTTTGCTTTGTGC+TGG + chr2.1:73341106-73341125 MS.gene02214:CDS 55.0%
GGTCACCAACCTTGAAACGC+TGG - chr2.1:73340714-73340733 None:intergenic 55.0%
GGTGACTTCTATGCAGGCGT+TGG + chr2.1:73341454-73341473 MS.gene02214:intron 55.0%
GGTGATTGCTAAGGGAGCTC+GGG + chr2.1:73340453-73340472 MS.gene02214:intron 55.0%
TGGGCAGCAAGAGATGCATC+TGG + chr2.1:73339951-73339970 MS.gene02214:CDS 55.0%
! TCTTGGTGGCCTCGGTCATA+TGG + chr2.1:73341198-73341217 MS.gene02214:CDS 55.0%
!! ACTGTTGCCGGAAGTGTTGC+AGG + chr2.1:73341728-73341747 MS.gene02214:CDS 55.0%
!! CTTGGAGTGGTTGGTCTTGG+TGG + chr2.1:73341184-73341203 MS.gene02214:CDS 55.0%
!! GGTTGGTGACCATGTTGGAG+TGG + chr2.1:73340723-73340742 MS.gene02214:CDS 55.0%
GCTGGGGTTGTGGCAAAGGT+TGG + chr2.1:73340679-73340698 MS.gene02214:CDS 60.0%
!! GTGGTTGGTCTTGGTGGCCT+CGG + chr2.1:73341190-73341209 MS.gene02214:CDS 60.0%
!! GTTGCCGGAAGTGTTGCAGG+TGG + chr2.1:73341731-73341750 MS.gene02214:CDS 60.0%
!! GAGGAGGCGCTGAGCCTTCT+TGG + chr2.1:73341286-73341305 MS.gene02214:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 73339915 73341916 73339915 ID=MS.gene02214
chr2.1 mRNA 73339915 73341916 73339915 ID=MS.gene02214.t1;Parent=MS.gene02214
chr2.1 exon 73339915 73340003 73339915 ID=MS.gene02214.t1.exon1;Parent=MS.gene02214.t1
chr2.1 CDS 73339915 73340003 73339915 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1 exon 73340466 73340579 73340466 ID=MS.gene02214.t1.exon2;Parent=MS.gene02214.t1
chr2.1 CDS 73340466 73340579 73340466 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1 exon 73340669 73340899 73340669 ID=MS.gene02214.t1.exon3;Parent=MS.gene02214.t1
chr2.1 CDS 73340669 73340899 73340669 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1 exon 73341066 73341351 73341066 ID=MS.gene02214.t1.exon4;Parent=MS.gene02214.t1
chr2.1 CDS 73341066 73341351 73341066 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1 exon 73341470 73341620 73341470 ID=MS.gene02214.t1.exon5;Parent=MS.gene02214.t1
chr2.1 CDS 73341470 73341620 73341470 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1 exon 73341720 73341916 73341720 ID=MS.gene02214.t1.exon6;Parent=MS.gene02214.t1
chr2.1 CDS 73341720 73341916 73341720 ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
Gene Sequence

>MS.gene02214

ATGAGTTCTGAAAGTGTTGGTGAAGATTGCCTTGCATGGGCAGCAAGAGATGCATCTGGAGTTCTGTCGCCTTACAAATTCAATCGCAGGTATATTTGTTATGTACCTCACATTCCGCTTTGTAGAATGGAGTAATTCTTTTAGGATTTAATTTTTATATATATACTGTCAGTGTAAAGGGTTTTTACACTTGTCACTCAATCATAACCGTTAAGTCATTAGAAACATTTGACTTTTATCATAATCGCTTCTAAAGTCACACACATGCTTGGCAGTGATGTGTTTGTTGACAGTGTAAAACTTATACAATCAGTGTATGAAAGTTAAACTCCGAGTTTTAAGTAAAATCTCATTTTCCTTTTGTTTCAAGGCAACCTTGCTACCAAAAGTTACTGGTTATTGCAGTTCCTAGTTTGATGCTACACTTTCATATCATATCAATTCAAATGCCCACATATAAGAATGTATGCATTTATGTATGACTTGCTGGCCATGTTACATTTTTCCAAAAAAAATTAAATCCTGACAATATGATAATGGTGATTGCTAAGGGAGCTCGGGAGTGAAGATGTTTATGTTAAAATTACACACTGTGGCGTTTGTTATGCTGATGTAATTTGGGCAAGGAATAAACATGGGGATTCAAAGTATCCTGTTGTGCCGGGGTAAGTTGTGAGCTATTCTTGTTGTGTGTATGGTTGAGAATGTTATATACATAAGCCTACTTATCTGTGTTTTTCTTTTTTCTTATCAGACACGAGATTGCTGGGGTTGTGGCAAAGGTTGGTCCTAATGTCCAGCGTTTCAAGGTTGGTGACCATGTTGGAGTGGGTACTTACATCAACTCATGCAGGGAATGTGAGTATTGTAACGATCGATTAGAAGTTCATTGTGTGAAGGGATCAGTTTACACCTTTAATGGTGTTGATTATGATGGTACAATTACAAAAGGAGGATACTCCACTTCCATTGTAGTACATGAAAGGTAAACTTAATAGATGGCTTGGTTCTGATTATGTTTTATGTGTTTTTATGAGACCTTCTCTTGCTGAAATTTATTATAAGCACTAAAACAACTTCTTTGATTTCATAATAATGGAGGTTGTAATTTCAGTTCATAACTTCTTTTATATATGGTCTTGCATATGCAGGTATTGCTTCTTGATACCTAAAAGCTATCCTTTAGCTTCAGCAGGTCCTTTGCTTTGTGCTGGAATCACTGTTTATTCTCCCATGATGCGACATAATATGAATCAACCTGGTAAGTCTCTTGGAGTGGTTGGTCTTGGTGGCCTCGGTCATATGGCAGTAAAATTTGGGAAGGCATTTGGTTTGCGTGTAACGGTTTTCAGCACTAGCATTTCAAAGAAAGAGGAGGCGCTGAGCCTTCTTGGTGCAGACCAATTTGTTGTTTCATCTAATCAAGAAGAGATGAGGGTAAGTATTATATGAACCATCTATACCATAGCATTTTCTTCAAACCATGAAGAGAATACTGATACTGGTGACATGTGCTTCTGTTTTTTTAACTAAACTGATGGTGACTTCTATGCAGGCGTTGGCTAAATCATTGGATTTTATAATTGACACGGCATCGGGTGATCACCCGTTTGATCCTTACATGACACTTCTGAAGATATCTGGTGTTCTGGTCTTAGTTGGGTTCCCAAGTGAAGTCAAATTCAGCCCTGCAAGCCTTAACTTAGGTAATGTTGCATTTGAAGTATAGAAGTTTTGTTTGAATAACAACTCGTTCTTTATATCGCTGGAACCAGAAGTAATATCCTTCATTCATCTGTTTTCAGGATCAAGAACTGTTGCCGGAAGTGTTGCAGGTGGTACAAAAGAAATACAGGAGATGGTCGACTTCTGTGCTGCAAATGGGATTCACCCAGAAATAGAACTGATTCCAATTGGGTACTCAAATGAAGCTCTTGAGAGGGTCGTAAATAAGGACGTGAAATATCGGTTTGTAATAGACATTGAAAATTCTCTCAAGTAG
Protein sequence

>MS.gene02214.t1

MSSESVGEDCLAWAARDASGVLSPYKFNRRELGSEDVYVKITHCGVCYADVIWARNKHGDSKYPVVPGHEIAGVVAKVGPNVQRFKVGDHVGVGTYINSCRECEYCNDRLEVHCVKGSVYTFNGVDYDGTITKGGYSTSIVVHERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSISKKEEALSLLGADQFVVSSNQEEMRALAKSLDFIIDTASGDHPFDPYMTLLKISGVLVLVGFPSEVKFSPASLNLGSRTVAGSVAGGTKEIQEMVDFCAANGIHPEIELIPIGYSNEALERVVNKDVKYRFVIDIENSLK