AlfalfaGEDB | MS.gene02214

M. sativa cultivar XinJiangDaYe / MS.gene02214

KEGG: K00083
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0008270 GO:0016491 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0055114
PFAM: PF00107.29 PF08240.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02214.t1	XP_024632469.1	97.2	355	10	0	1	355	1	355	1.30E-200	708.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02214.t1	Q9CAI3	75.7	354	85	1	2	355	3	355	3.5e-162	572.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02214.t1	G7IS81	97.2	355	10	0	1	355	52	406	9.4e-201	708.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene008956	MS.gene02214	PPI
MS.gene02214	MS.gene069153	PPI
MS.gene02214	MS.gene027823	PPI
MS.gene02214	MS.gene070869	PPI
MS.gene02214	MS.gene069152	PPI
MS.gene02214	MS.gene90766	PPI
MS.gene02214	MS.gene070872	PPI
MS.gene02214	MS.gene036724	PPI
MS.gene02214	MS.gene46316	PPI
MS.gene02214	MS.gene029589	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02214.t1	MTR_2g009810	97.183	355	10	0	1	355	52	406	0.0	716
MS.gene02214.t1	MTR_2g009820	89.113	248	23	2	112	355	7	254	1.70e-149	422
MS.gene02214.t1	MTR_5g031190	53.582	349	160	2	8	355	11	358	3.39e-124	362
MS.gene02214.t1	MTR_5g031360	54.155	349	158	2	8	355	13	360	1.34e-123	360
MS.gene02214.t1	MTR_5g031230	54.310	348	153	4	11	355	14	358	7.52e-120	350
MS.gene02214.t1	MTR_5g031310	53.314	347	158	3	11	355	15	359	7.02e-119	348
MS.gene02214.t1	MTR_5g031305	53.602	347	157	3	11	355	15	359	2.04e-118	347
MS.gene02214.t1	MTR_5g031390	52.450	347	161	3	11	355	14	358	3.92e-118	346
MS.gene02214.t1	MTR_5g031210	54.335	346	156	2	11	355	14	358	6.47e-118	345
MS.gene02214.t1	MTR_5g031250	52.312	346	163	2	11	355	14	358	5.07e-117	343

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02214.t1	AT1G72680	75.706	354	85	1	2	355	3	355	0.0	555
MS.gene02214.t1	AT4G37970	50.144	347	171	2	9	354	13	358	9.23e-114	335
MS.gene02214.t1	AT2G21890	52.874	348	157	4	11	354	8	352	1.63e-113	335
MS.gene02214.t1	AT4G39330	51.884	345	164	2	11	354	14	357	2.86e-113	334
MS.gene02214.t1	AT2G21730	52.738	347	160	4	11	354	8	353	2.67e-112	332
MS.gene02214.t1	AT4G37980	49.284	349	175	2	8	355	7	354	5.94e-104	310
MS.gene02214.t1	AT4G37990	48.138	349	179	2	8	355	7	354	1.40e-98	296
MS.gene02214.t1	AT3G19450	45.070	355	192	3	1	353	1	354	1.83e-91	278
MS.gene02214.t1	AT4G39330	51.034	290	140	2	11	299	14	302	1.79e-89	271
MS.gene02214.t1	AT4G34230	45.906	342	183	2	13	353	13	353	2.08e-88	270

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Find 105 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGACCTGCTGAAGCTAA+AGG	0.536529	2.1:-73341094	None:intergenic
AAATAGAACTGATTCCAATT+GGG	0.533719	2.1:+73341810	MS.gene02214:CDS
AATCAACCTGGTAAGTCTCT+TGG	0.459390	2.1:+73341166	MS.gene02214:CDS
AATGTCCAGCGTTTCAAGGT+TGG	0.539954	2.1:+73340706	MS.gene02214:CDS
ACAACTTACCCCGGCACAAC+AGG	0.440053	2.1:-73340566	None:intergenic
ACACGAGATTGCTGGGGTTG+TGG	0.469150	2.1:+73340669	MS.gene02214:CDS
ACCACTCCAAGAGACTTACC+AGG	0.671497	2.1:-73341172	None:intergenic
ACCTGGTAAGTCTCTTGGAG+TGG	0.618165	2.1:+73341171	MS.gene02214:CDS
ACCTTGAAACGCTGGACATT+AGG	0.363094	2.1:-73340703	None:intergenic
ACTCAAATGAAGCTCTTGAG+AGG	0.606095	2.1:+73341834	MS.gene02214:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTACTCCATTCTACAAAG+CGG	-	chr2.1:73340032-73340051	None:intergenic	30.0%
AGAAAATGCTATGGTATAGA+TGG	-	chr2.1:73341371-73341390	None:intergenic	30.0%
ATCACCATTATCATATTGTC+AGG	-	chr2.1:73340439-73340458	None:intergenic	30.0%
ATCTAATCAAGAAGAGATGA+GGG	+	chr2.1:73341330-73341349	MS.gene02214:CDS	30.0%
ATGCAACATTACCTAAGTTA+AGG	-	chr2.1:73341613-73341632	None:intergenic	30.0%
ATGGTTTGAAGAAAATGCTA+TGG	-	chr2.1:73341380-73341399	None:intergenic	30.0%
ATTAGAAGTTCATTGTGTGA+AGG	+	chr2.1:73340792-73340811	MS.gene02214:CDS	30.0%
ATTCATATTATGTCGCATCA+TGG	-	chr2.1:73341149-73341168	None:intergenic	30.0%
ATTGGAATCAGTTCTATTTC+TGG	-	chr2.1:73341809-73341828	None:intergenic	30.0%
GTCATATGGCAGTAAAATTT+GGG	+	chr2.1:73341212-73341231	MS.gene02214:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	73339915	73341916	73339915	ID=MS.gene02214
chr2.1	mRNA	73339915	73341916	73339915	ID=MS.gene02214.t1;Parent=MS.gene02214
chr2.1	exon	73339915	73340003	73339915	ID=MS.gene02214.t1.exon1;Parent=MS.gene02214.t1
chr2.1	CDS	73339915	73340003	73339915	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1	exon	73340466	73340579	73340466	ID=MS.gene02214.t1.exon2;Parent=MS.gene02214.t1
chr2.1	CDS	73340466	73340579	73340466	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1	exon	73340669	73340899	73340669	ID=MS.gene02214.t1.exon3;Parent=MS.gene02214.t1
chr2.1	CDS	73340669	73340899	73340669	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1	exon	73341066	73341351	73341066	ID=MS.gene02214.t1.exon4;Parent=MS.gene02214.t1
chr2.1	CDS	73341066	73341351	73341066	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1	exon	73341470	73341620	73341470	ID=MS.gene02214.t1.exon5;Parent=MS.gene02214.t1
chr2.1	CDS	73341470	73341620	73341470	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1
chr2.1	exon	73341720	73341916	73341720	ID=MS.gene02214.t1.exon6;Parent=MS.gene02214.t1
chr2.1	CDS	73341720	73341916	73341720	ID=cds.MS.gene02214.t1;Parent=MS.gene02214.t1

Gene Sequence

>MS.gene02214

ATGAGTTCTGAAAGTGTTGGTGAAGATTGCCTTGCATGGGCAGCAAGAGATGCATCTGGAGTTCTGTCGCCTTACAAATTCAATCGCAGGTATATTTGTTATGTACCTCACATTCCGCTTTGTAGAATGGAGTAATTCTTTTAGGATTTAATTTTTATATATATACTGTCAGTGTAAAGGGTTTTTACACTTGTCACTCAATCATAACCGTTAAGTCATTAGAAACATTTGACTTTTATCATAATCGCTTCTAAAGTCACACACATGCTTGGCAGTGATGTGTTTGTTGACAGTGTAAAACTTATACAATCAGTGTATGAAAGTTAAACTCCGAGTTTTAAGTAAAATCTCATTTTCCTTTTGTTTCAAGGCAACCTTGCTACCAAAAGTTACTGGTTATTGCAGTTCCTAGTTTGATGCTACACTTTCATATCATATCAATTCAAATGCCCACATATAAGAATGTATGCATTTATGTATGACTTGCTGGCCATGTTACATTTTTCCAAAAAAAATTAAATCCTGACAATATGATAATGGTGATTGCTAAGGGAGCTCGGGAGTGAAGATGTTTATGTTAAAATTACACACTGTGGCGTTTGTTATGCTGATGTAATTTGGGCAAGGAATAAACATGGGGATTCAAAGTATCCTGTTGTGCCGGGGTAAGTTGTGAGCTATTCTTGTTGTGTGTATGGTTGAGAATGTTATATACATAAGCCTACTTATCTGTGTTTTTCTTTTTTCTTATCAGACACGAGATTGCTGGGGTTGTGGCAAAGGTTGGTCCTAATGTCCAGCGTTTCAAGGTTGGTGACCATGTTGGAGTGGGTACTTACATCAACTCATGCAGGGAATGTGAGTATTGTAACGATCGATTAGAAGTTCATTGTGTGAAGGGATCAGTTTACACCTTTAATGGTGTTGATTATGATGGTACAATTACAAAAGGAGGATACTCCACTTCCATTGTAGTACATGAAAGGTAAACTTAATAGATGGCTTGGTTCTGATTATGTTTTATGTGTTTTTATGAGACCTTCTCTTGCTGAAATTTATTATAAGCACTAAAACAACTTCTTTGATTTCATAATAATGGAGGTTGTAATTTCAGTTCATAACTTCTTTTATATATGGTCTTGCATATGCAGGTATTGCTTCTTGATACCTAAAAGCTATCCTTTAGCTTCAGCAGGTCCTTTGCTTTGTGCTGGAATCACTGTTTATTCTCCCATGATGCGACATAATATGAATCAACCTGGTAAGTCTCTTGGAGTGGTTGGTCTTGGTGGCCTCGGTCATATGGCAGTAAAATTTGGGAAGGCATTTGGTTTGCGTGTAACGGTTTTCAGCACTAGCATTTCAAAGAAAGAGGAGGCGCTGAGCCTTCTTGGTGCAGACCAATTTGTTGTTTCATCTAATCAAGAAGAGATGAGGGTAAGTATTATATGAACCATCTATACCATAGCATTTTCTTCAAACCATGAAGAGAATACTGATACTGGTGACATGTGCTTCTGTTTTTTTAACTAAACTGATGGTGACTTCTATGCAGGCGTTGGCTAAATCATTGGATTTTATAATTGACACGGCATCGGGTGATCACCCGTTTGATCCTTACATGACACTTCTGAAGATATCTGGTGTTCTGGTCTTAGTTGGGTTCCCAAGTGAAGTCAAATTCAGCCCTGCAAGCCTTAACTTAGGTAATGTTGCATTTGAAGTATAGAAGTTTTGTTTGAATAACAACTCGTTCTTTATATCGCTGGAACCAGAAGTAATATCCTTCATTCATCTGTTTTCAGGATCAAGAACTGTTGCCGGAAGTGTTGCAGGTGGTACAAAAGAAATACAGGAGATGGTCGACTTCTGTGCTGCAAATGGGATTCACCCAGAAATAGAACTGATTCCAATTGGGTACTCAAATGAAGCTCTTGAGAGGGTCGTAAATAAGGACGTGAAATATCGGTTTGTAATAGACATTGAAAATTCTCTCAAGTAG

Protein sequence

>MS.gene02214.t1

MSSESVGEDCLAWAARDASGVLSPYKFNRRELGSEDVYVKITHCGVCYADVIWARNKHGDSKYPVVPGHEIAGVVAKVGPNVQRFKVGDHVGVGTYINSCRECEYCNDRLEVHCVKGSVYTFNGVDYDGTITKGGYSTSIVVHERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSISKKEEALSLLGADQFVVSSNQEEMRALAKSLDFIIDTASGDHPFDPYMTLLKISGVLVLVGFPSEVKFSPASLNLGSRTVAGSVAGGTKEIQEMVDFCAANGIHPEIELIPIGYSNEALERVVNKDVKYRFVIDIENSLK