AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98394


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98394.t1 MTR_2g046150 98.780 164 2 0 1 164 1 164 5.01e-118 330
MS.gene98394.t1 MTR_7g111900 46.479 142 75 1 6 146 2 143 5.66e-38 128
MS.gene98394.t1 MTR_1g084200 43.137 153 86 1 6 157 2 154 1.49e-36 124
MS.gene98394.t1 MTR_7g103200 43.382 136 76 1 6 140 2 137 2.05e-36 123
MS.gene98394.t1 MTR_2g019480 38.129 139 86 0 15 153 16 154 4.71e-32 112
MS.gene98394.t1 MTR_2g091215 40.909 132 78 0 11 142 3 134 9.45e-28 100
MS.gene98394.t1 MTR_2g091210 38.462 130 80 0 11 140 3 132 7.39e-27 98.6
MS.gene98394.t1 MTR_4g122930 36.986 146 85 4 7 147 3 146 5.99e-24 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98394.t1 AT4G24130 68.667 150 46 1 1 149 1 150 5.38e-72 214
MS.gene98394.t1 AT1G09310 46.207 145 76 2 7 150 3 146 1.29e-34 119
MS.gene98394.t1 AT1G56580 44.595 148 79 3 7 151 3 150 3.13e-34 118
MS.gene98394.t1 AT5G46230 37.121 132 83 0 9 140 8 139 1.61e-31 110
MS.gene98394.t1 AT1G30020 33.846 130 82 1 7 136 6 131 1.02e-25 96.3
MS.gene98394.t1 AT5G49600 34.694 147 85 4 7 143 1 146 1.38e-20 83.6

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTGTTCTTCCCTTAA+AGG 0.146605 2.4:+44870095 MS.gene98394:CDS
ACATTCAACAAGATCCTTTA+AGG 0.179628 2.4:-44870109 None:intergenic
AGAGTAAGCAATTGTTTGTT+TGG 0.287883 2.4:+44870280 MS.gene98394:CDS
TGTGGAAGAGTTAGAGAAAC+TGG 0.316514 2.4:+44870129 MS.gene98394:CDS
TCAATACTCATTTCAACTAT+AGG 0.386964 2.4:-44870306 None:intergenic
CTTTAAGGGAAGAACACCTT+TGG 0.399056 2.4:-44870094 None:intergenic
GAGTAAGCAATTGTTTGTTT+GGG 0.413681 2.4:+44870281 MS.gene98394:CDS
GAAGTTGCAATTGAAGGATA+AGG 0.421440 2.4:+44870431 MS.gene98394:CDS
CATTCAACAAGATCCTTTAA+GGG 0.423103 2.4:-44870108 None:intergenic
TTTAAGGGAAGAACACCTTT+GGG 0.434347 2.4:-44870093 None:intergenic
ATACAACTCTTAGAAGAGCT+TGG 0.448905 2.4:+44870066 MS.gene98394:CDS
TTTAAGACTCCTATGGGGAT+TGG 0.453850 2.4:+44870354 MS.gene98394:CDS
GTATGAGAAGTTGCAATTGA+AGG 0.453990 2.4:+44870425 MS.gene98394:CDS
AATTGAAGTTACTGCTTATG+TGG 0.459578 2.4:+44870224 MS.gene98394:CDS
ATAGTTGAAATGAGTATTGA+GGG 0.460794 2.4:+44870309 MS.gene98394:CDS
AAGCTCTTCTAAGAGTTGTA+TGG 0.469293 2.4:-44870064 None:intergenic
CCTTCAAAATAATGTTCACT+TGG 0.482969 2.4:-44870177 None:intergenic
TATAGTTGAAATGAGTATTG+AGG 0.501110 2.4:+44870308 MS.gene98394:CDS
TTAAAGGATCTTGTTGAATG+TGG 0.521582 2.4:+44870111 MS.gene98394:CDS
CTTCTAAGAGTTGTATGGAA+TGG 0.521965 2.4:-44870059 None:intergenic
GTTGCAATTGAAGGATAAGG+AGG 0.546376 2.4:+44870434 MS.gene98394:CDS
GCAGGTGCAGAAATAGTGTA+TGG 0.551940 2.4:+44870018 MS.gene98394:CDS
TACTTCATTTATGAGTGTTG+AGG 0.556318 2.4:+44870392 MS.gene98394:CDS
TTCAAGATGAAGAAAATGAG+TGG 0.567086 2.4:+44870252 MS.gene98394:CDS
ACAAAGACTCTTGTGAGCTA+CGG 0.601286 2.4:+44870201 MS.gene98394:CDS
GAAGAGCTTGGATTTCCCAA+AGG 0.601476 2.4:+44870078 MS.gene98394:CDS
ATGGTAACAAATGAACGTGC+AGG 0.612544 2.4:+44870000 MS.gene98394:CDS
AAAGACTTACCAATCCCCAT+AGG 0.653062 2.4:-44870363 None:intergenic
AATGGCTAGCAACAGCACAA+TGG 0.699467 2.4:+44869981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAATACTCATTTCAACTAT+AGG - chr2.4:44870309-44870328 None:intergenic 25.0%
!! TATAGTTGAAATGAGTATTG+AGG + chr2.4:44870308-44870327 MS.gene98394:CDS 25.0%
!!! ATAGTTGAAATGAGTATTGA+GGG + chr2.4:44870309-44870328 MS.gene98394:CDS 25.0%
!!! ATGTATTTTAAGACTCCTAT+GGG + chr2.4:44870348-44870367 MS.gene98394:CDS 25.0%
!!! TTTTGTTTGGATGAAACAAA+AGG + chr2.4:44870152-44870171 MS.gene98394:CDS 25.0%
AATTGAAGTTACTGCTTATG+TGG + chr2.4:44870224-44870243 MS.gene98394:CDS 30.0%
ACATTCAACAAGATCCTTTA+AGG - chr2.4:44870112-44870131 None:intergenic 30.0%
AGAGTAAGCAATTGTTTGTT+TGG + chr2.4:44870280-44870299 MS.gene98394:CDS 30.0%
CATTCAACAAGATCCTTTAA+GGG - chr2.4:44870111-44870130 None:intergenic 30.0%
CCTTCAAAATAATGTTCACT+TGG - chr2.4:44870180-44870199 None:intergenic 30.0%
GAGTAAGCAATTGTTTGTTT+GGG + chr2.4:44870281-44870300 MS.gene98394:CDS 30.0%
TTAAAGGATCTTGTTGAATG+TGG + chr2.4:44870111-44870130 MS.gene98394:CDS 30.0%
TTCAAGATGAAGAAAATGAG+TGG + chr2.4:44870252-44870271 MS.gene98394:CDS 30.0%
! TACTTCATTTATGAGTGTTG+AGG + chr2.4:44870392-44870411 MS.gene98394:CDS 30.0%
!!! ATGAGTATTGAGGGTTTTAA+TGG + chr2.4:44870318-44870337 MS.gene98394:CDS 30.0%
!!! CCAAGTGAACATTATTTTGA+AGG + chr2.4:44870177-44870196 MS.gene98394:CDS 30.0%
!!! GATGTATTTTAAGACTCCTA+TGG + chr2.4:44870347-44870366 MS.gene98394:CDS 30.0%
!!! TGTATTTTAAGACTCCTATG+GGG + chr2.4:44870349-44870368 MS.gene98394:CDS 30.0%
!!! TTAGAGAAACTGGTTTTGTT+TGG + chr2.4:44870139-44870158 MS.gene98394:CDS 30.0%
ATACAACTCTTAGAAGAGCT+TGG + chr2.4:44870066-44870085 MS.gene98394:CDS 35.0%
GAAGTTGCAATTGAAGGATA+AGG + chr2.4:44870431-44870450 MS.gene98394:CDS 35.0%
GTATGAGAAGTTGCAATTGA+AGG + chr2.4:44870425-44870444 MS.gene98394:CDS 35.0%
TTTAAGGGAAGAACACCTTT+GGG - chr2.4:44870096-44870115 None:intergenic 35.0%
! AAGCTCTTCTAAGAGTTGTA+TGG - chr2.4:44870067-44870086 None:intergenic 35.0%
! CTTCTAAGAGTTGTATGGAA+TGG - chr2.4:44870062-44870081 None:intergenic 35.0%
AAAGACTTACCAATCCCCAT+AGG - chr2.4:44870366-44870385 None:intergenic 40.0%
ACAAAGACTCTTGTGAGCTA+CGG + chr2.4:44870201-44870220 MS.gene98394:CDS 40.0%
ATGGTAACAAATGAACGTGC+AGG + chr2.4:44870000-44870019 MS.gene98394:CDS 40.0%
GTTGCAATTGAAGGATAAGG+AGG + chr2.4:44870434-44870453 MS.gene98394:CDS 40.0%
TGTGGAAGAGTTAGAGAAAC+TGG + chr2.4:44870129-44870148 MS.gene98394:CDS 40.0%
! CTTTAAGGGAAGAACACCTT+TGG - chr2.4:44870097-44870116 None:intergenic 40.0%
! TTTAAGACTCCTATGGGGAT+TGG + chr2.4:44870354-44870373 MS.gene98394:CDS 40.0%
!! CAAAGGTGTTCTTCCCTTAA+AGG + chr2.4:44870095-44870114 MS.gene98394:CDS 40.0%
GAAGAGCTTGGATTTCCCAA+AGG + chr2.4:44870078-44870097 MS.gene98394:CDS 45.0%
GCAGGTGCAGAAATAGTGTA+TGG + chr2.4:44870018-44870037 MS.gene98394:CDS 45.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 44869982 44870476 44869982 ID=MS.gene98394
chr2.4 mRNA 44869982 44870476 44869982 ID=MS.gene98394.t1;Parent=MS.gene98394
chr2.4 exon 44869982 44870476 44869982 ID=MS.gene98394.t1.exon1;Parent=MS.gene98394.t1
chr2.4 CDS 44869982 44870476 44869982 ID=cds.MS.gene98394.t1;Parent=MS.gene98394.t1
Gene Sequence

>MS.gene98394

ATGGCTAGCAACAGCACAATGGTAACAAATGAACGTGCAGGTGCAGAAATAGTGTATGGTTCTGAAGAATGTTTCAACCATTCCATACAACTCTTAGAAGAGCTTGGATTTCCCAAAGGTGTTCTTCCCTTAAAGGATCTTGTTGAATGTGGAAGAGTTAGAGAAACTGGTTTTGTTTGGATGAAACAAAAGGCACCAAGTGAACATTATTTTGAAGGTACAAAGACTCTTGTGAGCTACGGAATTGAAGTTACTGCTTATGTGGAGAAATTCAAGATGAAGAAAATGAGTGGAATTAAGAGTAAGCAATTGTTTGTTTGGGTGCCTATAGTTGAAATGAGTATTGAGGGTTTTAATGGAAAGAAGATGTATTTTAAGACTCCTATGGGGATTGGTAAGTCTTTTCATGTTACTTCATTTATGAGTGTTGAGGAAAAAGAAAAGTATGAGAAGTTGCAATTGAAGGATAAGGAGGTTGAGATCAAAGAAAATTAA

Protein sequence

>MS.gene98394.t1

MASNSTMVTNERAGAEIVYGSEECFNHSIQLLEELGFPKGVLPLKDLVECGRVRETGFVWMKQKAPSEHYFEGTKTLVSYGIEVTAYVEKFKMKKMSGIKSKQLFVWVPIVEMSIEGFNGKKMYFKTPMGIGKSFHVTSFMSVEEKEKYEKLQLKDKEVEIKEN