AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062042


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062042.t1 MTR_2g046150 98.780 164 2 0 1 164 1 164 5.01e-118 330
MS.gene062042.t1 MTR_7g111900 46.479 142 75 1 6 146 2 143 5.66e-38 128
MS.gene062042.t1 MTR_1g084200 43.137 153 86 1 6 157 2 154 1.49e-36 124
MS.gene062042.t1 MTR_7g103200 43.382 136 76 1 6 140 2 137 2.05e-36 123
MS.gene062042.t1 MTR_2g019480 38.129 139 86 0 15 153 16 154 4.71e-32 112
MS.gene062042.t1 MTR_2g091215 40.909 132 78 0 11 142 3 134 9.45e-28 100
MS.gene062042.t1 MTR_2g091210 38.462 130 80 0 11 140 3 132 7.39e-27 98.6
MS.gene062042.t1 MTR_4g122930 36.986 146 85 4 7 147 3 146 5.99e-24 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062042.t1 AT4G24130 68.667 150 46 1 1 149 1 150 5.38e-72 214
MS.gene062042.t1 AT1G09310 46.207 145 76 2 7 150 3 146 1.29e-34 119
MS.gene062042.t1 AT1G56580 44.595 148 79 3 7 151 3 150 3.13e-34 118
MS.gene062042.t1 AT5G46230 37.121 132 83 0 9 140 8 139 1.61e-31 110
MS.gene062042.t1 AT1G30020 33.846 130 82 1 7 136 6 131 1.02e-25 96.3
MS.gene062042.t1 AT5G49600 34.694 147 85 4 7 143 1 146 1.38e-20 83.6

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTGTTCTTCCCTTAA+AGG 0.146605 2.3:+44606877 MS.gene062042:CDS
ACATTCAACAAGATCCTTTA+AGG 0.179628 2.3:-44606891 None:intergenic
AGAGTAAGCAATTGTTTGTT+TGG 0.287883 2.3:+44607062 MS.gene062042:CDS
TGTGGAAGAGTTAGAGAAAC+TGG 0.316514 2.3:+44606911 MS.gene062042:CDS
TCAATACTCATTTCAACTAT+AGG 0.386964 2.3:-44607088 None:intergenic
CTTTAAGGGAAGAACACCTT+TGG 0.399056 2.3:-44606876 None:intergenic
GAGTAAGCAATTGTTTGTTT+GGG 0.413681 2.3:+44607063 MS.gene062042:CDS
GAAGTTGCAATTGAAGGATA+AGG 0.421440 2.3:+44607213 MS.gene062042:CDS
CATTCAACAAGATCCTTTAA+GGG 0.423103 2.3:-44606890 None:intergenic
TTTAAGGGAAGAACACCTTT+GGG 0.434347 2.3:-44606875 None:intergenic
ATACAACTCTTAGAAGAGCT+TGG 0.448905 2.3:+44606848 MS.gene062042:CDS
TTTAAGACTCCTATGGGGAT+TGG 0.453850 2.3:+44607136 MS.gene062042:CDS
GTATGAGAAGTTGCAATTGA+AGG 0.453990 2.3:+44607207 MS.gene062042:CDS
AATTGAAGTTACTGCTTATG+TGG 0.459578 2.3:+44607006 MS.gene062042:CDS
ATAGTTGAAATGAGTATTGA+GGG 0.460794 2.3:+44607091 MS.gene062042:CDS
AAGCTCTTCTAAGAGTTGTA+TGG 0.469293 2.3:-44606846 None:intergenic
CCTTCAAAATAATGTTCACT+TGG 0.482969 2.3:-44606959 None:intergenic
TATAGTTGAAATGAGTATTG+AGG 0.501110 2.3:+44607090 MS.gene062042:CDS
TTAAAGGATCTTGTTGAATG+TGG 0.521582 2.3:+44606893 MS.gene062042:CDS
CTTCTAAGAGTTGTATGGAA+TGG 0.521965 2.3:-44606841 None:intergenic
GTTGCAATTGAAGGATAAGG+AGG 0.546376 2.3:+44607216 MS.gene062042:CDS
GCAGGTGCAGAAATAGTGTA+TGG 0.551940 2.3:+44606800 MS.gene062042:CDS
TACTTCATTTATGAGTGTTG+AGG 0.556318 2.3:+44607174 MS.gene062042:CDS
TTCAAGATGAAGAAAATGAG+TGG 0.567086 2.3:+44607034 MS.gene062042:CDS
ACAAAGACTCTTGTGAGCTA+CGG 0.601286 2.3:+44606983 MS.gene062042:CDS
GAAGAGCTTGGATTTCCCAA+AGG 0.601476 2.3:+44606860 MS.gene062042:CDS
ATGGTAACAAATGAACGTGC+AGG 0.612544 2.3:+44606782 MS.gene062042:CDS
AAAGACTTACCAATCCCCAT+AGG 0.653062 2.3:-44607145 None:intergenic
AATGGCTAGCAACAGCACAA+TGG 0.699467 2.3:+44606763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAATACTCATTTCAACTAT+AGG - chr2.3:44607091-44607110 None:intergenic 25.0%
!! TATAGTTGAAATGAGTATTG+AGG + chr2.3:44607090-44607109 MS.gene062042:CDS 25.0%
!!! ATAGTTGAAATGAGTATTGA+GGG + chr2.3:44607091-44607110 MS.gene062042:CDS 25.0%
!!! ATGTATTTTAAGACTCCTAT+GGG + chr2.3:44607130-44607149 MS.gene062042:CDS 25.0%
!!! TTTTGTTTGGATGAAACAAA+AGG + chr2.3:44606934-44606953 MS.gene062042:CDS 25.0%
AATTGAAGTTACTGCTTATG+TGG + chr2.3:44607006-44607025 MS.gene062042:CDS 30.0%
ACATTCAACAAGATCCTTTA+AGG - chr2.3:44606894-44606913 None:intergenic 30.0%
AGAGTAAGCAATTGTTTGTT+TGG + chr2.3:44607062-44607081 MS.gene062042:CDS 30.0%
CATTCAACAAGATCCTTTAA+GGG - chr2.3:44606893-44606912 None:intergenic 30.0%
CCTTCAAAATAATGTTCACT+TGG - chr2.3:44606962-44606981 None:intergenic 30.0%
GAGTAAGCAATTGTTTGTTT+GGG + chr2.3:44607063-44607082 MS.gene062042:CDS 30.0%
TTAAAGGATCTTGTTGAATG+TGG + chr2.3:44606893-44606912 MS.gene062042:CDS 30.0%
TTCAAGATGAAGAAAATGAG+TGG + chr2.3:44607034-44607053 MS.gene062042:CDS 30.0%
! TACTTCATTTATGAGTGTTG+AGG + chr2.3:44607174-44607193 MS.gene062042:CDS 30.0%
!!! ATGAGTATTGAGGGTTTTAA+TGG + chr2.3:44607100-44607119 MS.gene062042:CDS 30.0%
!!! CCAAGTGAACATTATTTTGA+AGG + chr2.3:44606959-44606978 MS.gene062042:CDS 30.0%
!!! GATGTATTTTAAGACTCCTA+TGG + chr2.3:44607129-44607148 MS.gene062042:CDS 30.0%
!!! TGTATTTTAAGACTCCTATG+GGG + chr2.3:44607131-44607150 MS.gene062042:CDS 30.0%
!!! TTAGAGAAACTGGTTTTGTT+TGG + chr2.3:44606921-44606940 MS.gene062042:CDS 30.0%
ATACAACTCTTAGAAGAGCT+TGG + chr2.3:44606848-44606867 MS.gene062042:CDS 35.0%
GAAGTTGCAATTGAAGGATA+AGG + chr2.3:44607213-44607232 MS.gene062042:CDS 35.0%
GTATGAGAAGTTGCAATTGA+AGG + chr2.3:44607207-44607226 MS.gene062042:CDS 35.0%
TTTAAGGGAAGAACACCTTT+GGG - chr2.3:44606878-44606897 None:intergenic 35.0%
! AAGCTCTTCTAAGAGTTGTA+TGG - chr2.3:44606849-44606868 None:intergenic 35.0%
! CTTCTAAGAGTTGTATGGAA+TGG - chr2.3:44606844-44606863 None:intergenic 35.0%
AAAGACTTACCAATCCCCAT+AGG - chr2.3:44607148-44607167 None:intergenic 40.0%
ACAAAGACTCTTGTGAGCTA+CGG + chr2.3:44606983-44607002 MS.gene062042:CDS 40.0%
ATGGTAACAAATGAACGTGC+AGG + chr2.3:44606782-44606801 MS.gene062042:CDS 40.0%
GTTGCAATTGAAGGATAAGG+AGG + chr2.3:44607216-44607235 MS.gene062042:CDS 40.0%
TGTGGAAGAGTTAGAGAAAC+TGG + chr2.3:44606911-44606930 MS.gene062042:CDS 40.0%
! CTTTAAGGGAAGAACACCTT+TGG - chr2.3:44606879-44606898 None:intergenic 40.0%
! TTTAAGACTCCTATGGGGAT+TGG + chr2.3:44607136-44607155 MS.gene062042:CDS 40.0%
!! CAAAGGTGTTCTTCCCTTAA+AGG + chr2.3:44606877-44606896 MS.gene062042:CDS 40.0%
GAAGAGCTTGGATTTCCCAA+AGG + chr2.3:44606860-44606879 MS.gene062042:CDS 45.0%
GCAGGTGCAGAAATAGTGTA+TGG + chr2.3:44606800-44606819 MS.gene062042:CDS 45.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 44606764 44607258 44606764 ID=MS.gene062042
chr2.3 mRNA 44606764 44607258 44606764 ID=MS.gene062042.t1;Parent=MS.gene062042
chr2.3 exon 44606764 44607258 44606764 ID=MS.gene062042.t1.exon1;Parent=MS.gene062042.t1
chr2.3 CDS 44606764 44607258 44606764 ID=cds.MS.gene062042.t1;Parent=MS.gene062042.t1
Gene Sequence

>MS.gene062042

ATGGCTAGCAACAGCACAATGGTAACAAATGAACGTGCAGGTGCAGAAATAGTGTATGGTTCTGAAGAATGTTTCAACCATTCCATACAACTCTTAGAAGAGCTTGGATTTCCCAAAGGTGTTCTTCCCTTAAAGGATCTTGTTGAATGTGGAAGAGTTAGAGAAACTGGTTTTGTTTGGATGAAACAAAAGGCACCAAGTGAACATTATTTTGAAGGTACAAAGACTCTTGTGAGCTACGGAATTGAAGTTACTGCTTATGTGGAGAAATTCAAGATGAAGAAAATGAGTGGAATTAAGAGTAAGCAATTGTTTGTTTGGGTGCCTATAGTTGAAATGAGTATTGAGGGTTTTAATGGAAAGAAGATGTATTTTAAGACTCCTATGGGGATTGGTAAGTCTTTTCATGTTACTTCATTTATGAGTGTTGAGGAAAAAGAAAAGTATGAGAAGTTGCAATTGAAGGATAAGGAGGTTGAGATCAAAGAAAATTAA

Protein sequence

>MS.gene062042.t1

MASNSTMVTNERAGAEIVYGSEECFNHSIQLLEELGFPKGVLPLKDLVECGRVRETGFVWMKQKAPSEHYFEGTKTLVSYGIEVTAYVEKFKMKKMSGIKSKQLFVWVPIVEMSIEGFNGKKMYFKTPMGIGKSFHVTSFMSVEEKEKYEKLQLKDKEVEIKEN