AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07222


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07222.t1 MTR_1g088450 98.319 119 2 0 1 119 1 119 5.80e-83 238
MS.gene07222.t1 MTR_1g083430 89.076 119 12 1 2 119 7 125 1.68e-63 189
MS.gene07222.t1 MTR_7g112770 94.444 108 6 0 12 119 17 124 1.15e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07222.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene07222.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene07222.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene07222.t1 AT5G27770 86.735 98 13 0 11 108 16 113 1.42e-49 154
MS.gene07222.t1 AT1G02830 75.229 109 27 0 2 110 9 117 9.39e-46 144

Find 27 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTGGAGAAGTTTCTTC+AGG 0.314117 1.4:-70241835 MS.gene07222:CDS
GCCAGTTGAGGATAAGATTA+TGG 0.328511 1.4:-70241865 MS.gene07222:CDS
ATAGATATTGCGGTCCTTGT+TGG 0.377160 1.4:+70240709 None:intergenic
TCCATAATCTTATCCTCAAC+TGG 0.404476 1.4:+70241864 None:intergenic
CGGAGTGAAGGGTAAGAAGA+AGG 0.409364 1.4:-70241916 MS.gene07222:CDS
ATCGGAGGTAACAGTGATCT+TGG 0.422181 1.4:+70241746 None:intergenic
GATTATGGACATCGCTTCCC+TGG 0.434039 1.4:-70241850 MS.gene07222:CDS
CGCGAAAATGAGCGGAGTGA+AGG 0.462475 1.4:-70241928 None:intergenic
TCTCCTGAAGAAACTTCTCC+AGG 0.463361 1.4:+70241832 None:intergenic
AAAAGCACAATGTGAGGGAT+TGG 0.474548 1.4:-70240748 MS.gene07222:CDS
CTTCAACATTGCTGAGAATG+AGG 0.480373 1.4:-70240675 MS.gene07222:CDS
CCGATAGCAATTTCTCCAAA+AGG 0.482446 1.4:-70241728 MS.gene07222:intron
ATTAAAGTTGGTGGTAAAGC+TGG 0.482585 1.4:-70241807 MS.gene07222:CDS
GCGAAAATGAGCGGAGTGAA+GGG 0.509201 1.4:-70241927 None:intergenic
CTTCAGGAGAGGATTAAAGT+TGG 0.530224 1.4:-70241819 MS.gene07222:CDS
CAGGAGAGGATTAAAGTTGG+TGG 0.545199 1.4:-70241816 MS.gene07222:CDS
GGTGGTAAAGCTGGTGCTCT+CGG 0.555561 1.4:-70241798 MS.gene07222:CDS
CGATTGCGCGAAGCCAGTTG+AGG 0.561050 1.4:-70241877 MS.gene07222:CDS
TGGAGAAGTTTCTTCAGGAG+AGG 0.565476 1.4:-70241830 MS.gene07222:CDS
GGAGTGAAGGGTAAGAAGAA+GGG 0.572006 1.4:-70241915 MS.gene07222:CDS
ATCTCAACTCATAGATATTG+CGG 0.582807 1.4:+70240699 None:intergenic
AGTCTCAACGCGAAAATGAG+CGG 0.596462 1.4:-70241936 None:intergenic
TTTGGAGAAATTGCTATCGG+AGG 0.603460 1.4:+70241731 None:intergenic
TCGTGTTATTGCATCCAACA+AGG 0.613064 1.4:-70240723 MS.gene07222:CDS
TTCAACATTGCTGAGAATGA+GGG 0.613248 1.4:-70240674 MS.gene07222:CDS
CTCCTGAAGAAACTTCTCCA+GGG 0.621359 1.4:+70241833 None:intergenic
TTTGTCACGAGTAACAGTAA+CGG 0.687075 1.4:+70241770 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTATGTCTTAATTTTTTT+TGG - chr1.4:70241697-70241716 MS.gene07222:intron 15.0%
!! AAAATTATGTTGTTAGTGAA+TGG - chr1.4:70241103-70241122 MS.gene07222:intron 20.0%
!! AAATTATGTTGTTAGTGAAT+GGG - chr1.4:70241104-70241123 MS.gene07222:intron 20.0%
!! ATTTGACATTATTATGATTC+TGG - chr1.4:70241577-70241596 MS.gene07222:intron 20.0%
!!! TATTTGTTGATTAATGTGTT+AGG - chr1.4:70240890-70240909 MS.gene07222:intron 20.0%
! AAAAATAGTTAAACTGAGCT+GGG + chr1.4:70241628-70241647 None:intergenic 25.0%
! ATATTTAGTGTATGTTTGGA+TGG - chr1.4:70241154-70241173 MS.gene07222:intron 25.0%
! TAAAAATAGTTAAACTGAGC+TGG + chr1.4:70241629-70241648 None:intergenic 25.0%
! TATTTAGTGTATGTTTGGAT+GGG - chr1.4:70241155-70241174 MS.gene07222:intron 25.0%
! TCGTATATTTAGTGTATGTT+TGG - chr1.4:70241150-70241169 MS.gene07222:intron 25.0%
ATACAAGTCTTCTTCTTCTT+TGG - chr1.4:70241720-70241739 MS.gene07222:intron 30.0%
ATCTCAACTCATAGATATTG+CGG + chr1.4:70241883-70241902 None:intergenic 30.0%
CATGTGAACATTTCATTTAC+AGG + chr1.4:70240946-70240965 None:intergenic 30.0%
CATGTGTTTGAGTAATTATG+TGG - chr1.4:70240962-70240981 MS.gene07222:intron 30.0%
GTCAAATTCTATTCACATAC+AGG + chr1.4:70241564-70241583 None:intergenic 30.0%
! AAGATGAAGAAGTTACCTTT+TGG + chr1.4:70240869-70240888 None:intergenic 30.0%
! ACTGTAAGTCACCATAATTT+TGG + chr1.4:70241229-70241248 None:intergenic 30.0%
! CTGTAAGTCACCATAATTTT+GGG + chr1.4:70241228-70241247 None:intergenic 30.0%
!!! TTTTGTTATTTTCTACCGTG+AGG - chr1.4:70241349-70241368 MS.gene07222:intron 30.0%
ACAGGAATACTAGACAATAG+TGG + chr1.4:70241546-70241565 None:intergenic 35.0%
ATTGAATATTGAGACAGTGC+TGG - chr1.4:70241010-70241029 MS.gene07222:intron 35.0%
CATCAATTGCAGGTTTCAAT+TGG + chr1.4:70241270-70241289 None:intergenic 35.0%
GGACAAAAATAATCACACGA+AGG + chr1.4:70241757-70241776 None:intergenic 35.0%
TCCATAATCTTATCCTCAAC+TGG + chr1.4:70240718-70240737 None:intergenic 35.0%
TGTCATGAGAATCCTTAAGT+TGG + chr1.4:70241475-70241494 None:intergenic 35.0%
TTCAACATTGCTGAGAATGA+GGG - chr1.4:70241905-70241924 MS.gene07222:CDS 35.0%
TTTGTCACGAGTAACAGTAA+CGG + chr1.4:70240812-70240831 None:intergenic 35.0%
! ACTTGAAAAAGCACAATGTG+AGG - chr1.4:70241825-70241844 MS.gene07222:CDS 35.0%
! ATGATTTGTGTAAATGGTCC+TGG - chr1.4:70241517-70241536 MS.gene07222:intron 35.0%
! CGTAGGTTTTCCCAAAATTA+TGG - chr1.4:70241215-70241234 MS.gene07222:intron 35.0%
! CTTGAAAAAGCACAATGTGA+GGG - chr1.4:70241826-70241845 MS.gene07222:CDS 35.0%
! GCGAGTATGATTTGTGTAAA+TGG - chr1.4:70241511-70241530 MS.gene07222:intron 35.0%
!! ACACTATGCTTGTTGTGAAA+GGG - chr1.4:70241393-70241412 MS.gene07222:intron 35.0%
!! ATGTGGCTTTGAAATGTTGA+TGG - chr1.4:70240979-70240998 MS.gene07222:intron 35.0%
!! ATTAAAGTTGGTGGTAAAGC+TGG - chr1.4:70240772-70240791 MS.gene07222:CDS 35.0%
!! CCTTTTGGAGAAATTGCTAT+CGG + chr1.4:70240854-70240873 None:intergenic 35.0%
!!! GTGCTTTTTCAAGTACTTCT+TGG + chr1.4:70241819-70241838 None:intergenic 35.0%
AGGTCTAGTTGTTGCTGTAT+GGG - chr1.4:70241369-70241388 MS.gene07222:intron 40.0%
ATAGATATTGCGGTCCTTGT+TGG + chr1.4:70241873-70241892 None:intergenic 40.0%
CCGATAGCAATTTCTCCAAA+AGG - chr1.4:70240851-70240870 MS.gene07222:intron 40.0%
CTTCAACATTGCTGAGAATG+AGG - chr1.4:70241904-70241923 MS.gene07222:CDS 40.0%
CTTCAGGAGAGGATTAAAGT+TGG - chr1.4:70240760-70240779 MS.gene07222:CDS 40.0%
GAAAATGCCATTACCAGTCT+TGG - chr1.4:70241422-70241441 MS.gene07222:intron 40.0%
GCCAGTTGAGGATAAGATTA+TGG - chr1.4:70240714-70240733 MS.gene07222:CDS 40.0%
GGTCAAGTACTTCAAATACC+TGG + chr1.4:70241798-70241817 None:intergenic 40.0%
GTACTTCAAATACCTGGCAA+AGG + chr1.4:70241792-70241811 None:intergenic 40.0%
TAATCAACACTACCTTTGCC+AGG - chr1.4:70241777-70241796 MS.gene07222:CDS 40.0%
TGACTACACACATCAATTGC+AGG + chr1.4:70241280-70241299 None:intergenic 40.0%
TGGGAAAACCTACGAATTCA+GGG + chr1.4:70241209-70241228 None:intergenic 40.0%
TTAGTGTATGTTTGGATGGG+TGG - chr1.4:70241158-70241177 MS.gene07222:intron 40.0%
TTGGGAAAACCTACGAATTC+AGG + chr1.4:70241210-70241229 None:intergenic 40.0%
TTTGGAGAAATTGCTATCGG+AGG + chr1.4:70240851-70240870 None:intergenic 40.0%
! AAAAGCACAATGTGAGGGAT+TGG - chr1.4:70241831-70241850 MS.gene07222:CDS 40.0%
! TCGTGTTATTGCATCCAACA+AGG - chr1.4:70241856-70241875 MS.gene07222:CDS 40.0%
!! CACACTATGCTTGTTGTGAA+AGG - chr1.4:70241392-70241411 MS.gene07222:intron 40.0%
!! TGAGAATCCTTAAGTTGGCA+CGG + chr1.4:70241470-70241489 None:intergenic 40.0%
!! TGGCAAAGGTAGTGTTGATT+AGG + chr1.4:70241778-70241797 None:intergenic 40.0%
ACAGCAACAACTAGACCTCA+CGG + chr1.4:70241367-70241386 None:intergenic 45.0%
ATCGGAGGTAACAGTGATCT+TGG + chr1.4:70240836-70240855 None:intergenic 45.0%
CAAACATCACAAGCCAAGAC+TGG + chr1.4:70241438-70241457 None:intergenic 45.0%
CAGGAGAGGATTAAAGTTGG+TGG - chr1.4:70240763-70240782 MS.gene07222:CDS 45.0%
CTCCTGAAGAAACTTCTCCA+GGG + chr1.4:70240749-70240768 None:intergenic 45.0%
GAGGTCTAGTTGTTGCTGTA+TGG - chr1.4:70241368-70241387 MS.gene07222:intron 45.0%
GGAGTGAAGGGTAAGAAGAA+GGG - chr1.4:70240664-70240683 MS.gene07222:CDS 45.0%
TCACAAGCCAAGACTGGTAA+TGG + chr1.4:70241432-70241451 None:intergenic 45.0%
TCTCCTGAAGAAACTTCTCC+AGG + chr1.4:70240750-70240769 None:intergenic 45.0%
TGGAGAAGTTTCTTCAGGAG+AGG - chr1.4:70240749-70240768 MS.gene07222:CDS 45.0%
TTCCCTGGAGAAGTTTCTTC+AGG - chr1.4:70240744-70240763 MS.gene07222:CDS 45.0%
TTCTGGTGCAGTTACTGAAC+TGG - chr1.4:70241594-70241613 MS.gene07222:intron 45.0%
!! ACTAGACAATAGTGGACACC+AGG + chr1.4:70241538-70241557 None:intergenic 45.0%
!! GAGAATCCTTAAGTTGGCAC+GGG + chr1.4:70241469-70241488 None:intergenic 45.0%
CGGAGTGAAGGGTAAGAAGA+AGG - chr1.4:70240663-70240682 MS.gene07222:CDS 50.0%
CGTTAGCACCCTGAATTCGT+AGG - chr1.4:70241198-70241217 MS.gene07222:intron 50.0%
GATTATGGACATCGCTTCCC+TGG - chr1.4:70240729-70240748 MS.gene07222:CDS 50.0%
TGCATTCCCGTGCCAACTTA+AGG - chr1.4:70241460-70241479 MS.gene07222:intron 50.0%
GGTGGTAAAGCTGGTGCTCT+CGG - chr1.4:70240781-70240800 MS.gene07222:CDS 55.0%
CGATTGCGCGAAGCCAGTTG+AGG - chr1.4:70240702-70240721 MS.gene07222:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70240658 70241943 70240658 ID=MS.gene07222
chr1.4 mRNA 70240658 70241943 70240658 ID=MS.gene07222.t1;Parent=MS.gene07222
chr1.4 exon 70241729 70241943 70241729 ID=MS.gene07222.t1.exon1;Parent=MS.gene07222.t1
chr1.4 CDS 70241729 70241943 70241729 ID=cds.MS.gene07222.t1;Parent=MS.gene07222.t1
chr1.4 exon 70240658 70240802 70240658 ID=MS.gene07222.t1.exon2;Parent=MS.gene07222.t1
chr1.4 CDS 70240658 70240802 70240658 ID=cds.MS.gene07222.t1;Parent=MS.gene07222.t1
Gene Sequence

>MS.gene07222

ATGAGCGGAGTGAAGGGTAAGAAGAAGGGAACAAGCTTCGTGATCGATTGCGCGAAGCCAGTTGAGGATAAGATTATGGACATCGCTTCCCTGGAGAAGTTTCTTCAGGAGAGGATTAAAGTTGGTGGTAAAGCTGGTGCTCTCGGTGATTCCGTTACTGTTACTCGTGACAAATCCAAGATCACTGTTACCTCCGATAGCAATTTCTCCAAAAGGTAACTTCTTCATCTTTTATTTGTTGATTAATGTGTTAGGTCAAATATTTGTAAGAGTTAGATCTAGTATCCTGTAAATGAAATGTTCACATGTGTTTGAGTAATTATGTGGCTTTGAAATGTTGATGGAGAATTCGATTGAATATTGAGACAGTGCTGGAATGAAATATGCTTCATGTAATTGTATTGATTATATCATGTGCGATTTACTATTTTTTCTGTTTCATTTTAAAATTATGTTGTTAGTGAATGGGTTAGATTCTTTGAATTTAACTACTCGTATATTTAGTGTATGTTTGGATGGGTGGTGTGTTTGTCATAATCTCGTTAGCACCCTGAATTCGTAGGTTTTCCCAAAATTATGGTGACTTACAGTGATTTTGAAATTTCACTGCCAATTGAAACCTGCAATTGATGTGTGTAGTCAACTCTTCAAAAGTGCACTTTTCAACATAAAATCAACATCTATGTGTTTCTTTTGTTATTTTCTACCGTGAGGTCTAGTTGTTGCTGTATGGGCACACTATGCTTGTTGTGAAAGGGATGTAAGAAAATGCCATTACCAGTCTTGGCTTGTGATGTTTGCATGCATTCCCGTGCCAACTTAAGGATTCTCATGACAATTTTTACATTATTGAGCGAGTATGATTTGTGTAAATGGTCCTGGTGTCCACTATTGTCTAGTATTCCTGTATGTGAATAGAATTTGACATTATTATGATTCTGGTGCAGTTACTGAACTGGATTCTTGTCCCAGCTCAGTTTAACTATTTTTAGTAACTGTTTGTTCAAATCTGTATGCAATCAATTGAAATCATTTTTTTCTTTATGTCTTAATTTTTTTTGGATACAAGTCTTCTTCTTCTTTGGTTTTTCTATTGCCTTCGTGTGATTATTTTTGTCCTAATCAACACTACCTTTGCCAGGTATTTGAAGTACTTGACCAAGAAGTACTTGAAAAAGCACAATGTGAGGGATTGGCTTCGTGTTATTGCATCCAACAAGGACCGCAATATCTATGAGTTGAGATACTTCAACATTGCTGAGAATGAGGGTGAAGATGAAGACTAA

Protein sequence

>MS.gene07222.t1

MSGVKGKKKGTSFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVTRDKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNIYELRYFNIAENEGEDED