Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09032.t1 | XP_013446572.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.30E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09032.t1 | I3SCJ8 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 2.4e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene09032 | 0.829789 | 4.23E-55 | -1.69E-46 |
| MS.gene049341 | MS.gene09032 | 0.820013 | 8.59E-53 | -1.69E-46 |
| MS.gene049756 | MS.gene09032 | 0.819406 | 1.18E-52 | -1.69E-46 |
| MS.gene04984 | MS.gene09032 | 0.807615 | 4.64E-50 | -1.69E-46 |
| MS.gene049857 | MS.gene09032 | 0.802496 | 5.46E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene09032 | 0.824215 | 9.11E-54 | -1.69E-46 |
| MS.gene050114 | MS.gene09032 | 0.807588 | 4.70E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene09032 | 0.905384 | 4.54E-80 | -1.69E-46 |
| MS.gene050288 | MS.gene09032 | 0.808484 | 3.03E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene09032 | 0.832025 | 1.20E-55 | -1.69E-46 |
| MS.gene050320 | MS.gene09032 | 0.864585 | 1.09E-64 | -1.69E-46 |
| MS.gene050437 | MS.gene09032 | 0.828393 | 9.22E-55 | -1.69E-46 |
| MS.gene050689 | MS.gene09032 | 0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene09032 | 0.830267 | 3.23E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene09032 | 0.809913 | 1.50E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene09032 | 0.875716 | 2.48E-68 | -1.69E-46 |
| MS.gene051003 | MS.gene09032 | 0.819808 | 9.56E-53 | -1.69E-46 |
| MS.gene051063 | MS.gene09032 | 0.871018 | 9.41E-67 | -1.69E-46 |
| MS.gene051233 | MS.gene09032 | 0.80634 | 8.62E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene09032 | 0.815014 | 1.15E-51 | -1.69E-46 |
| MS.gene051245 | MS.gene09032 | 0.881585 | 2.12E-70 | -1.69E-46 |
| MS.gene051328 | MS.gene09032 | 0.83703 | 6.61E-57 | -1.69E-46 |
| MS.gene051330 | MS.gene09032 | 0.825951 | 3.54E-54 | -1.69E-46 |
| MS.gene05134 | MS.gene09032 | 0.816876 | 4.41E-52 | -1.69E-46 |
| MS.gene051451 | MS.gene09032 | 0.811886 | 5.59E-51 | -1.69E-46 |
| MS.gene051633 | MS.gene09032 | 0.84164 | 4.20E-58 | -1.69E-46 |
| MS.gene051746 | MS.gene09032 | 0.865731 | 4.77E-65 | -1.69E-46 |
| MS.gene051748 | MS.gene09032 | 0.882659 | 8.66E-71 | -1.69E-46 |
| MS.gene051756 | MS.gene09032 | 0.877538 | 5.80E-69 | -1.69E-46 |
| MS.gene051757 | MS.gene09032 | 0.860328 | 2.23E-63 | -1.69E-46 |
| MS.gene051850 | MS.gene09032 | 0.860179 | 2.47E-63 | -1.69E-46 |
| MS.gene051903 | MS.gene09032 | 0.901834 | 1.79E-78 | -1.69E-46 |
| MS.gene052133 | MS.gene09032 | 0.813913 | 2.01E-51 | -1.69E-46 |
| MS.gene052202 | MS.gene09032 | 0.834678 | 2.61E-56 | -1.69E-46 |
| MS.gene052286 | MS.gene09032 | 0.835961 | 1.24E-56 | -1.69E-46 |
| MS.gene052302 | MS.gene09032 | 0.817759 | 2.79E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene09032 | 0.808454 | 3.07E-50 | -1.69E-46 |
| MS.gene052856 | MS.gene09032 | 0.813494 | 2.49E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene09032 | 0.861783 | 8.05E-64 | -1.69E-46 |
| MS.gene053058 | MS.gene09032 | 0.813687 | 2.26E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene09032 | 0.884162 | 2.43E-71 | -1.69E-46 |
| MS.gene053381 | MS.gene09032 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene09032 | 0.804836 | 1.78E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene09032 | 0.816987 | 4.16E-52 | -1.69E-46 |
| MS.gene05380 | MS.gene09032 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene053952 | MS.gene09032 | 0.837965 | 3.81E-57 | -1.69E-46 |
| MS.gene054403 | MS.gene09032 | 0.824463 | 7.96E-54 | -1.69E-46 |
| MS.gene054823 | MS.gene09032 | 0.806861 | 6.70E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene09032 | 0.808087 | 3.68E-50 | -1.69E-46 |
| MS.gene055162 | MS.gene09032 | 0.809054 | 2.29E-50 | -1.69E-46 |
| MS.gene055174 | MS.gene09032 | 0.819747 | 9.87E-53 | -1.69E-46 |
| MS.gene055311 | MS.gene09032 | 0.812283 | 4.58E-51 | -1.69E-46 |
| MS.gene055424 | MS.gene09032 | 0.844456 | 7.48E-59 | -1.69E-46 |
| MS.gene055441 | MS.gene09032 | 0.828723 | 7.67E-55 | -1.69E-46 |
| MS.gene055591 | MS.gene09032 | -0.810095 | 1.37E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene09032 | 0.805003 | 1.65E-49 | -1.69E-46 |
| MS.gene056432 | MS.gene09032 | 0.840098 | 1.07E-57 | -1.69E-46 |
| MS.gene056580 | MS.gene09032 | 0.848164 | 7.29E-60 | -1.69E-46 |
| MS.gene056601 | MS.gene09032 | 0.848991 | 4.31E-60 | -1.69E-46 |
| MS.gene056631 | MS.gene09032 | 0.889941 | 1.54E-73 | -1.69E-46 |
| MS.gene056752 | MS.gene09032 | 0.808432 | 3.11E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene09032 | 0.835205 | 1.92E-56 | -1.69E-46 |
| MS.gene056787 | MS.gene09032 | 0.813337 | 2.69E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene09032 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene09032 | 0.880505 | 5.20E-70 | -1.69E-46 |
| MS.gene057094 | MS.gene09032 | 0.892905 | 1.03E-74 | -1.69E-46 |
| MS.gene057140 | MS.gene09032 | 0.856557 | 2.97E-62 | -1.69E-46 |
| MS.gene057234 | MS.gene09032 | 0.913499 | 5.73E-84 | -1.69E-46 |
| MS.gene057451 | MS.gene09032 | -0.818406 | 1.99E-52 | -1.69E-46 |
| MS.gene057452 | MS.gene09032 | -0.830272 | 3.22E-55 | -1.69E-46 |
| MS.gene057705 | MS.gene09032 | 0.815369 | 9.58E-52 | -1.69E-46 |
| MS.gene05781 | MS.gene09032 | 0.803819 | 2.91E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene09032 | 0.835321 | 1.80E-56 | -1.69E-46 |
| MS.gene058054 | MS.gene09032 | 0.839889 | 1.21E-57 | -1.69E-46 |
| MS.gene058055 | MS.gene09032 | 0.88359 | 3.95E-71 | -1.69E-46 |
| MS.gene058084 | MS.gene09032 | 0.801692 | 7.99E-49 | -1.69E-46 |
| MS.gene05809 | MS.gene09032 | 0.826269 | 2.98E-54 | -1.69E-46 |
| MS.gene058182 | MS.gene09032 | 0.836356 | 9.82E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene09032 | 0.890799 | 7.10E-74 | -1.69E-46 |
| MS.gene058646 | MS.gene09032 | 0.821702 | 3.51E-53 | -1.69E-46 |
| MS.gene058654 | MS.gene09032 | 0.845446 | 4.04E-59 | -1.69E-46 |
| MS.gene058906 | MS.gene09032 | 0.917355 | 5.87E-86 | -1.69E-46 |
| MS.gene059171 | MS.gene09032 | 0.829133 | 6.10E-55 | -1.69E-46 |
| MS.gene059898 | MS.gene09032 | 0.866681 | 2.39E-65 | -1.69E-46 |
| MS.gene06001 | MS.gene09032 | 0.892692 | 1.26E-74 | -1.69E-46 |
| MS.gene06011 | MS.gene09032 | 0.824551 | 7.59E-54 | -1.69E-46 |
| MS.gene060297 | MS.gene09032 | 0.81271 | 3.70E-51 | -1.69E-46 |
| MS.gene060463 | MS.gene09032 | 0.844558 | 7.02E-59 | -1.69E-46 |
| MS.gene060465 | MS.gene09032 | 0.847701 | 9.79E-60 | -1.69E-46 |
| MS.gene060702 | MS.gene09032 | 0.829745 | 4.33E-55 | -1.69E-46 |
| MS.gene060869 | MS.gene09032 | 0.810098 | 1.36E-50 | -1.69E-46 |
| MS.gene061145 | MS.gene09032 | 0.852082 | 5.83E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09032.t1 | MTR_8g080630 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.22e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09032.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
| MS.gene09032.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
Find 29 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTACCCTATGCGACCTTA+TGG | 0.310546 | 4.2:+14453820 | MS.gene09032:CDS |
| AATCTACGATCCCTTTCATT+TGG | 0.347988 | 4.2:-14453742 | None:intergenic |
| CTATGCGACCTTATGGGTTC+TGG | 0.350279 | 4.2:+14453827 | MS.gene09032:CDS |
| AGTGAAAATGGCTTTCATAA+TGG | 0.354869 | 4.2:+14453681 | None:intergenic |
| ATGAAAGGGATCGTAGATTC+AGG | 0.366060 | 4.2:+14453746 | MS.gene09032:CDS |
| AATGGAATTCGCTGAGAATC+TGG | 0.378487 | 4.2:+14453699 | MS.gene09032:CDS |
| GTTACCCTATGCGACCTTAT+GGG | 0.431260 | 4.2:+14453821 | MS.gene09032:CDS |
| CCTCATTTCGACGACGAGGC+AGG | 0.443390 | 4.2:-14453946 | None:intergenic |
| GAATCTGGTTCTGAAATTGA+TGG | 0.457082 | 4.2:+14453714 | MS.gene09032:CDS |
| CCCAAGCAAGCTGAGAGTTA+TGG | 0.460911 | 4.2:-14453861 | None:intergenic |
| GCCATAACTCTCAGCTTGCT+TGG | 0.460971 | 4.2:+14453860 | MS.gene09032:CDS |
| CCATAACTCTCAGCTTGCTT+GGG | 0.463504 | 4.2:+14453861 | MS.gene09032:CDS |
| GCTCAGATTAGTCAAGTTGC+CGG | 0.484929 | 4.2:+14453886 | MS.gene09032:CDS |
| TGTGAAAGGATTTCATCATA+AGG | 0.496680 | 4.2:-14453919 | None:intergenic |
| CCTGCCTCGTCGTCGAAATG+AGG | 0.497822 | 4.2:+14453946 | MS.gene09032:CDS |
| CTCATTTCGACGACGAGGCA+GGG | 0.505092 | 4.2:-14453945 | None:intergenic |
| TCAAATGTCCAGAACCCATA+AGG | 0.513007 | 4.2:-14453835 | None:intergenic |
| TAGTCAAGTTGCCGGTCGTA+GGG | 0.521121 | 4.2:+14453894 | MS.gene09032:CDS |
| GATGGAAGATCCAAATGAAA+GGG | 0.524216 | 4.2:+14453732 | MS.gene09032:CDS |
| TGATGGAAGATCCAAATGAA+AGG | 0.535018 | 4.2:+14453731 | MS.gene09032:CDS |
| AGATAGATGCGCGAAGACGA+AGG | 0.541002 | 4.2:+14453789 | MS.gene09032:CDS |
| TGAAAGGGATCGTAGATTCA+GGG | 0.568833 | 4.2:+14453747 | MS.gene09032:CDS |
| CAGAACCCATAAGGTCGCAT+AGG | 0.571457 | 4.2:-14453826 | None:intergenic |
| GCGCGAAGACGAAGGAGATG+TGG | 0.571625 | 4.2:+14453797 | MS.gene09032:CDS |
| TTAGTCAAGTTGCCGGTCGT+AGG | 0.572671 | 4.2:+14453893 | MS.gene09032:CDS |
| ATCATAAGGATCCCTACGAC+CGG | 0.573881 | 4.2:-14453905 | None:intergenic |
| AGAACCCATAAGGTCGCATA+GGG | 0.582926 | 4.2:-14453825 | None:intergenic |
| ACGAGGCAGGGTAATGTGAA+AGG | 0.598363 | 4.2:-14453933 | None:intergenic |
| AAAACCTCATTTCGACGACG+AGG | 0.717022 | 4.2:-14453950 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTGAAAGGATTTCATCATA+AGG | - | chr4.2:14453922-14453941 | None:intergenic | 30.0% | |
| AATCTACGATCCCTTTCATT+TGG | - | chr4.2:14453745-14453764 | None:intergenic | 35.0% | |
| GATGGAAGATCCAAATGAAA+GGG | + | chr4.2:14453732-14453751 | MS.gene09032:CDS | 35.0% | |
| TGATGGAAGATCCAAATGAA+AGG | + | chr4.2:14453731-14453750 | MS.gene09032:CDS | 35.0% | |
| !! | GAATCTGGTTCTGAAATTGA+TGG | + | chr4.2:14453714-14453733 | MS.gene09032:CDS | 35.0% |
| AATGGAATTCGCTGAGAATC+TGG | + | chr4.2:14453699-14453718 | MS.gene09032:CDS | 40.0% | |
| ATGAAAGGGATCGTAGATTC+AGG | + | chr4.2:14453746-14453765 | MS.gene09032:CDS | 40.0% | |
| TCAAATGTCCAGAACCCATA+AGG | - | chr4.2:14453838-14453857 | None:intergenic | 40.0% | |
| TGAAAGGGATCGTAGATTCA+GGG | + | chr4.2:14453747-14453766 | MS.gene09032:CDS | 40.0% | |
| AGAACCCATAAGGTCGCATA+GGG | - | chr4.2:14453828-14453847 | None:intergenic | 45.0% | |
| AGTTACCCTATGCGACCTTA+TGG | + | chr4.2:14453820-14453839 | MS.gene09032:CDS | 45.0% | |
| ATCATAAGGATCCCTACGAC+CGG | - | chr4.2:14453908-14453927 | None:intergenic | 45.0% | |
| GTTACCCTATGCGACCTTAT+GGG | + | chr4.2:14453821-14453840 | MS.gene09032:CDS | 45.0% | |
| ! | CCATAACTCTCAGCTTGCTT+GGG | + | chr4.2:14453861-14453880 | MS.gene09032:CDS | 45.0% |
| ! | GCTCAGATTAGTCAAGTTGC+CGG | + | chr4.2:14453886-14453905 | MS.gene09032:CDS | 45.0% |
| ACGAGGCAGGGTAATGTGAA+AGG | - | chr4.2:14453936-14453955 | None:intergenic | 50.0% | |
| AGATAGATGCGCGAAGACGA+AGG | + | chr4.2:14453789-14453808 | MS.gene09032:CDS | 50.0% | |
| CAGAACCCATAAGGTCGCAT+AGG | - | chr4.2:14453829-14453848 | None:intergenic | 50.0% | |
| CCCAAGCAAGCTGAGAGTTA+TGG | - | chr4.2:14453864-14453883 | None:intergenic | 50.0% | |
| CTATGCGACCTTATGGGTTC+TGG | + | chr4.2:14453827-14453846 | MS.gene09032:CDS | 50.0% | |
| ! | GCCATAACTCTCAGCTTGCT+TGG | + | chr4.2:14453860-14453879 | MS.gene09032:CDS | 50.0% |
| ! | TAGTCAAGTTGCCGGTCGTA+GGG | + | chr4.2:14453894-14453913 | MS.gene09032:CDS | 50.0% |
| ! | TTAGTCAAGTTGCCGGTCGT+AGG | + | chr4.2:14453893-14453912 | MS.gene09032:CDS | 50.0% |
| GCGCGAAGACGAAGGAGATG+TGG | + | chr4.2:14453797-14453816 | MS.gene09032:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 14453688 | 14453966 | 14453688 | ID=MS.gene09032 |
| chr4.2 | mRNA | 14453688 | 14453966 | 14453688 | ID=MS.gene09032.t1;Parent=MS.gene09032 |
| chr4.2 | exon | 14453688 | 14453966 | 14453688 | ID=MS.gene09032.t1.exon1;Parent=MS.gene09032.t1 |
| chr4.2 | CDS | 14453688 | 14453966 | 14453688 | ID=cds.MS.gene09032.t1;Parent=MS.gene09032.t1 |
| Gene Sequence |
| Protein sequence |
