AlfalfaGEDB | MS.gene27991

M. sativa cultivar XinJiangDaYe / MS.gene27991

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27991.t1	XP_013446710.1	100	64	0	0	1	64	1	64	9.40E-25	122.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27991.t1	A0A072TTH9	100.0	64	0	0	1	64	1	64	6.8e-25	122.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene27991	0.836128	1.12E-56	-1.69E-46
MS.gene049340	MS.gene27991	0.807909	4.02E-50	-1.69E-46
MS.gene049341	MS.gene27991	0.856543	3.00E-62	-1.69E-46
MS.gene049382	MS.gene27991	0.824364	8.40E-54	-1.69E-46
MS.gene049501	MS.gene27991	0.80247	5.52E-49	-1.69E-46
MS.gene049756	MS.gene27991	0.83709	6.38E-57	-1.69E-46
MS.gene04984	MS.gene27991	0.812141	4.92E-51	-1.69E-46
MS.gene049887	MS.gene27991	0.830016	3.72E-55	-1.69E-46
MS.gene049979	MS.gene27991	0.816775	4.65E-52	-1.69E-46
MS.gene050277	MS.gene27991	0.882637	8.81E-71	-1.69E-46
MS.gene050303	MS.gene27991	0.822415	2.40E-53	-1.69E-46
MS.gene050438	MS.gene27991	0.846408	2.21E-59	-1.69E-46
MS.gene050586	MS.gene27991	0.803496	3.39E-49	-1.69E-46
MS.gene050719	MS.gene27991	0.85659	2.90E-62	-1.69E-46
MS.gene050721	MS.gene27991	0.862075	6.55E-64	-1.69E-46
MS.gene050724	MS.gene27991	0.843383	1.45E-58	-1.69E-46
MS.gene050780	MS.gene27991	0.82416	9.38E-54	-1.69E-46
MS.gene050807	MS.gene27991	0.823636	1.25E-53	-1.69E-46
MS.gene050815	MS.gene27991	0.873674	1.22E-67	-1.69E-46
MS.gene050838	MS.gene27991	0.827643	1.40E-54	-1.69E-46
MS.gene051063	MS.gene27991	0.872757	2.49E-67	-1.69E-46
MS.gene051088	MS.gene27991	0.809492	1.84E-50	-1.69E-46
MS.gene051243	MS.gene27991	0.838865	2.23E-57	-1.69E-46
MS.gene051245	MS.gene27991	0.866272	3.22E-65	-1.69E-46
MS.gene051247	MS.gene27991	0.812202	4.77E-51	-1.69E-46
MS.gene051328	MS.gene27991	0.856818	2.49E-62	-1.69E-46
MS.gene051330	MS.gene27991	0.817938	2.54E-52	-1.69E-46
MS.gene051349	MS.gene27991	0.820296	7.39E-53	-1.69E-46
MS.gene05134	MS.gene27991	0.822853	1.90E-53	-1.69E-46
MS.gene051577	MS.gene27991	0.816178	6.32E-52	-1.69E-46
MS.gene051626	MS.gene27991	0.801942	7.10E-49	-1.69E-46
MS.gene051633	MS.gene27991	0.849588	2.94E-60	-1.69E-46
MS.gene051746	MS.gene27991	0.851671	7.62E-61	-1.69E-46
MS.gene051748	MS.gene27991	0.846946	1.58E-59	-1.69E-46
MS.gene051756	MS.gene27991	0.829007	6.55E-55	-1.69E-46
MS.gene051757	MS.gene27991	0.893617	5.32E-75	-1.69E-46
MS.gene051789	MS.gene27991	0.81536	9.62E-52	-1.69E-46
MS.gene051850	MS.gene27991	0.807724	4.40E-50	-1.69E-46
MS.gene051903	MS.gene27991	0.85353	2.25E-61	-1.69E-46
MS.gene052012	MS.gene27991	0.806722	7.17E-50	-1.69E-46
MS.gene052020	MS.gene27991	0.802193	6.30E-49	-1.69E-46
MS.gene052133	MS.gene27991	0.827853	1.24E-54	-1.69E-46
MS.gene052202	MS.gene27991	0.817594	3.04E-52	-1.69E-46
MS.gene052286	MS.gene27991	0.84793	8.46E-60	-1.69E-46
MS.gene052302	MS.gene27991	0.858093	1.04E-62	-1.69E-46
MS.gene052376	MS.gene27991	0.857093	2.06E-62	-1.69E-46
MS.gene052665	MS.gene27991	0.828644	8.02E-55	-1.69E-46
MS.gene052856	MS.gene27991	0.83712	6.27E-57	-1.69E-46
MS.gene052879	MS.gene27991	0.821383	4.16E-53	-1.69E-46
MS.gene052880	MS.gene27991	0.80832	3.28E-50	-1.69E-46
MS.gene052896	MS.gene27991	0.819134	1.36E-52	-1.69E-46
MS.gene052947	MS.gene27991	0.841002	6.19E-58	-1.69E-46
MS.gene053008	MS.gene27991	0.801307	9.58E-49	-1.69E-46
MS.gene053074	MS.gene27991	0.811223	7.80E-51	-1.69E-46
MS.gene053109	MS.gene27991	0.825102	5.63E-54	-1.69E-46
MS.gene053132	MS.gene27991	0.807351	5.27E-50	-1.69E-46
MS.gene053437	MS.gene27991	0.828535	8.52E-55	-1.69E-46
MS.gene053630	MS.gene27991	0.814125	1.81E-51	-1.69E-46
MS.gene053952	MS.gene27991	0.800331	1.52E-48	-1.69E-46
MS.gene054185	MS.gene27991	0.807785	4.27E-50	-1.69E-46
MS.gene054250	MS.gene27991	0.800116	1.68E-48	-1.69E-46
MS.gene054472	MS.gene27991	0.816934	4.28E-52	-1.69E-46
MS.gene055133	MS.gene27991	0.826123	3.22E-54	-1.69E-46
MS.gene055162	MS.gene27991	0.84474	6.27E-59	-1.69E-46
MS.gene055424	MS.gene27991	0.802076	6.66E-49	-1.69E-46
MS.gene055441	MS.gene27991	0.802206	6.26E-49	-1.69E-46
MS.gene055466	MS.gene27991	0.804149	2.48E-49	-1.69E-46
MS.gene055468	MS.gene27991	0.812927	3.31E-51	-1.69E-46
MS.gene055512	MS.gene27991	0.821477	3.96E-53	-1.69E-46
MS.gene055580	MS.gene27991	-0.807786	4.26E-50	-1.69E-46
MS.gene055591	MS.gene27991	-0.834884	2.32E-56	-1.69E-46
MS.gene055623	MS.gene27991	0.809734	1.63E-50	-1.69E-46
MS.gene056043	MS.gene27991	-0.822448	2.36E-53	-1.69E-46
MS.gene056069	MS.gene27991	-0.836677	8.13E-57	-1.69E-46
MS.gene056432	MS.gene27991	0.866635	2.47E-65	-1.69E-46
MS.gene056580	MS.gene27991	0.81862	1.78E-52	-1.69E-46
MS.gene056601	MS.gene27991	0.825767	3.92E-54	-1.69E-46
MS.gene056631	MS.gene27991	0.892473	1.54E-74	-1.69E-46
MS.gene056760	MS.gene27991	0.86819	7.85E-66	-1.69E-46
MS.gene057057	MS.gene27991	0.864685	1.02E-64	-1.69E-46
MS.gene057094	MS.gene27991	0.844005	9.88E-59	-1.69E-46
MS.gene057140	MS.gene27991	0.884709	1.52E-71	-1.69E-46
MS.gene057234	MS.gene27991	0.879601	1.09E-69	-1.69E-46
MS.gene057260	MS.gene27991	0.836811	7.52E-57	-1.69E-46
MS.gene057705	MS.gene27991	0.808875	2.50E-50	-1.69E-46
MS.gene05795	MS.gene27991	0.814302	1.65E-51	-1.69E-46
MS.gene058055	MS.gene27991	0.854477	1.20E-61	-1.69E-46
MS.gene058084	MS.gene27991	0.856133	3.95E-62	-1.69E-46
MS.gene05809	MS.gene27991	0.835276	1.84E-56	-1.69E-46
MS.gene058168	MS.gene27991	0.826161	3.16E-54	-1.69E-46
MS.gene058182	MS.gene27991	0.865239	6.82E-65	-1.69E-46
MS.gene058238	MS.gene27991	0.851009	1.17E-60	-1.69E-46
MS.gene058798	MS.gene27991	0.815776	7.77E-52	-1.69E-46
MS.gene058851	MS.gene27991	0.825045	5.81E-54	-1.69E-46
MS.gene058906	MS.gene27991	0.99532	4.12E-215	-1.69E-46
MS.gene058982	MS.gene27991	0.813929	2.00E-51	-1.69E-46
MS.gene059171	MS.gene27991	0.805887	1.07E-49	-1.69E-46
MS.gene059249	MS.gene27991	0.834421	3.03E-56	-1.69E-46
MS.gene059713	MS.gene27991	0.812564	3.98E-51	-1.69E-46
MS.gene059898	MS.gene27991	0.871023	9.37E-67	-1.69E-46
MS.gene059937	MS.gene27991	0.823275	1.51E-53	-1.69E-46
MS.gene06001	MS.gene27991	0.904349	1.35E-79	-1.69E-46
MS.gene060165	MS.gene27991	0.815435	9.26E-52	-1.69E-46
MS.gene060429	MS.gene27991	0.841714	4.02E-58	-1.69E-46
MS.gene060586	MS.gene27991	0.810376	1.19E-50	-1.69E-46
MS.gene060619	MS.gene27991	0.841189	5.53E-58	-1.69E-46
MS.gene060907	MS.gene27991	0.803912	2.78E-49	-1.69E-46
MS.gene060917	MS.gene27991	0.802656	5.06E-49	-1.69E-46
MS.gene061145	MS.gene27991	0.821371	4.18E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27991.t1	MTR_8g089640	100.000	64	0	0	1	64	1	64	5.64e-42	130

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27991.t1	AT1G67785	77.419	62	13	1	1	62	1	61	2.22e-30	101

Find 19 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTCAGAGAACAATTAATT+CGG	0.374447	4.4:+11674890	MS.gene27991:CDS
CGCTCTTCATCAGGACGAAA+AGG	0.423832	4.4:+11674502	MS.gene27991:CDS
GTGAAGGTTGCAACGTACTT+TGG	0.431142	4.4:+11674422	MS.gene27991:CDS
CCATGGACAAAGTCCATGTC+TGG	0.443394	4.4:+11674477	MS.gene27991:CDS
TGTCTGGATCGCTCTTCATC+AGG	0.451814	4.4:+11674493	MS.gene27991:CDS
GGTCAGAGAACAATTAATTC+GGG	0.478951	4.4:+11674891	MS.gene27991:CDS
AACGTACTTTGGAATGTCCT+TGG	0.500806	4.4:+11674433	MS.gene27991:CDS
AGAAGGAAGCGGAGATCAGA+AGG	0.504547	4.4:+11674869	MS.gene27991:CDS
CCAGACATGGACTTTGTCCA+TGG	0.515687	4.4:-11674477	None:intergenic
TGTGTTGCAGCAAGAGAGGT+TGG	0.535566	4.4:+11674846	MS.gene27991:intron
AGAGAGGTTGGAGAAGGAAG+CGG	0.542879	4.4:+11674858	MS.gene27991:CDS
CCAGAAAACGAATGCTGCCA+AGG	0.569490	4.4:-11674450	None:intergenic
ATATGAAGAATCCTTCTGAC+TGG	0.574630	4.4:-11674920	None:intergenic
ATAAAGATTCAAAATGGTGA+AGG	0.596896	4.4:+11674406	None:intergenic
GCAGCAAGAGAGGTTGGAGA+AGG	0.597767	4.4:+11674852	MS.gene27991:intron
GATGAAGAGCGATCCAGACA+TGG	0.613241	4.4:-11674490	None:intergenic
TCGGGAGCAAGCCAGTCAGA+AGG	0.625525	4.4:+11674909	MS.gene27991:CDS
GAAGGAAGCGGAGATCAGAA+GGG	0.632205	4.4:+11674870	MS.gene27991:CDS
GTAATGTGTTGCAGCAAGAG+AGG	0.669103	4.4:+11674842	MS.gene27991:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATATTTTGTGTTATTTAAT+TGG	+	chr4.4:11674818-11674837	MS.gene27991:intron	10.0%
!!	AGTGAAATTGAAATTAAATA+TGG	-	chr4.4:11674604-11674623	None:intergenic	15.0%
!!	ATTAATTTCACTCATTTAAG+AGG	+	chr4.4:11674641-11674660	MS.gene27991:intron	20.0%
!	AATATGGAAATTATCCGAAA+AGG	-	chr4.4:11674588-11674607	None:intergenic	25.0%
!	TCGTTATAAGATGAAATCTA+GGG	-	chr4.4:11674683-11674702	None:intergenic	25.0%
!	TTCGTTATAAGATGAAATCT+AGG	-	chr4.4:11674684-11674703	None:intergenic	25.0%
	ATGAAGAATTCGAAGAAGTA+AGG	-	chr4.4:11674536-11674555	None:intergenic	30.0%
	TGAAGAATTCGAAGAAGTAA+GGG	-	chr4.4:11674535-11674554	None:intergenic	30.0%
!	TGTGGTGAATTTGTTCTTTA+AGG	+	chr4.4:11674747-11674766	MS.gene27991:intron	30.0%
!!!	CAATTCTTGCTATTCCTTTT+CGG	+	chr4.4:11674571-11674590	MS.gene27991:intron	30.0%
	ATATGAAGAATCCTTCTGAC+TGG	-	chr4.4:11674923-11674942	None:intergenic	35.0%
	GAAGAATTCGAAGAAGTAAG+GGG	-	chr4.4:11674534-11674553	None:intergenic	35.0%
	GGGTCAGAGAACAATTAATT+CGG	+	chr4.4:11674890-11674909	MS.gene27991:CDS	35.0%
	GGTCAGAGAACAATTAATTC+GGG	+	chr4.4:11674891-11674910	MS.gene27991:CDS	35.0%
	TACTTATGTGTGCAATGTTG+AGG	+	chr4.4:11674708-11674727	MS.gene27991:intron	35.0%
!	GGTAACTGAGTTATTGACTG+TGG	+	chr4.4:11674729-11674748	MS.gene27991:intron	40.0%
!!	AACGTACTTTGGAATGTCCT+TGG	+	chr4.4:11674433-11674452	MS.gene27991:CDS	40.0%
!!	ATTCGTTTTCTGGCAATCCA+TGG	+	chr4.4:11674460-11674479	MS.gene27991:CDS	40.0%
	GTAATGTGTTGCAGCAAGAG+AGG	+	chr4.4:11674842-11674861	MS.gene27991:intron	45.0%
	GTGAAGGTTGCAACGTACTT+TGG	+	chr4.4:11674422-11674441	MS.gene27991:CDS	45.0%
	AGAAGGAAGCGGAGATCAGA+AGG	+	chr4.4:11674869-11674888	MS.gene27991:CDS	50.0%
	AGAGAGGTTGGAGAAGGAAG+CGG	+	chr4.4:11674858-11674877	MS.gene27991:CDS	50.0%
	CCAGAAAACGAATGCTGCCA+AGG	-	chr4.4:11674453-11674472	None:intergenic	50.0%
	CCAGACATGGACTTTGTCCA+TGG	-	chr4.4:11674480-11674499	None:intergenic	50.0%
	CCATGGACAAAGTCCATGTC+TGG	+	chr4.4:11674477-11674496	MS.gene27991:CDS	50.0%
	CGCTCTTCATCAGGACGAAA+AGG	+	chr4.4:11674502-11674521	MS.gene27991:CDS	50.0%
	GAAGGAAGCGGAGATCAGAA+GGG	+	chr4.4:11674870-11674889	MS.gene27991:CDS	50.0%
	GATGAAGAGCGATCCAGACA+TGG	-	chr4.4:11674493-11674512	None:intergenic	50.0%
	TGTCTGGATCGCTCTTCATC+AGG	+	chr4.4:11674493-11674512	MS.gene27991:CDS	50.0%
	TGTGTTGCAGCAAGAGAGGT+TGG	+	chr4.4:11674846-11674865	MS.gene27991:intron	50.0%
!	CCTTGGCAGCATTCGTTTTC+TGG	+	chr4.4:11674450-11674469	MS.gene27991:CDS	50.0%
	GCAGCAAGAGAGGTTGGAGA+AGG	+	chr4.4:11674852-11674871	MS.gene27991:intron	55.0%
	TCGGGAGCAAGCCAGTCAGA+AGG	+	chr4.4:11674909-11674928	MS.gene27991:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	11674419	11674945	11674419	ID=MS.gene27991
chr4.4	mRNA	11674419	11674945	11674419	ID=MS.gene27991.t1;Parent=MS.gene27991
chr4.4	exon	11674419	11674523	11674419	ID=MS.gene27991.t1.exon1;Parent=MS.gene27991.t1
chr4.4	CDS	11674419	11674523	11674419	ID=cds.MS.gene27991.t1;Parent=MS.gene27991.t1
chr4.4	exon	11674856	11674945	11674856	ID=MS.gene27991.t1.exon2;Parent=MS.gene27991.t1
chr4.4	CDS	11674856	11674945	11674856	ID=cds.MS.gene27991.t1;Parent=MS.gene27991.t1

Gene Sequence

>MS.gene27991

ATGGTGAAGGTTGCAACGTACTTTGGAATGTCCTTGGCAGCATTCGTTTTCTGGCAATCCATGGACAAAGTCCATGTCTGGATCGCTCTTCATCAGGACGAAAAGGTTCTTACCCCTTACTTCTTCGAATTCTTCATCTTCTAATCAAAATTCAATTCTTGCTATTCCTTTTCGGATAATTTCCATATTTAATTTCAATTTCACTATATTTGTTTTGTATTGATTAATTTCACTCATTTAAGAGGAAACAAAAATTTCAAACCCTAGATTTCATCTTATAACGAATTGTTACTTATGTGTGCAATGTTGAGGTAACTGAGTTATTGACTGTGGTGAATTTGTTCTTTAAGGATTTTGATTCGTATATTCATTCTGCAGTATTATTATGAATATTTGATCAATATTTTGTGTTATTTAATTGGTGTAATGTGTTGCAGCAAGAGAGGTTGGAGAAGGAAGCGGAGATCAGAAGGGTCAGAGAACAATTAATTCGGGAGCAAGCCAGTCAGAAGGATTCTTCATATTGA

Protein sequence

>MS.gene27991.t1

MVKVATYFGMSLAAFVFWQSMDKVHVWIALHQDEKQERLEKEAEIRRVREQLIREQASQKDSSY