AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20740.t1 MTR_1g088450 98.319 119 2 0 1 119 1 119 5.80e-83 238
MS.gene20740.t1 MTR_1g083430 89.076 119 12 1 2 119 7 125 1.68e-63 189
MS.gene20740.t1 MTR_7g112770 94.444 108 6 0 12 119 17 124 1.15e-61 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20740.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene20740.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene20740.t1 AT3G05560 87.931 116 14 0 4 119 9 124 5.82e-63 187
MS.gene20740.t1 AT5G27770 86.735 98 13 0 11 108 16 113 1.42e-49 154
MS.gene20740.t1 AT1G02830 75.229 109 27 0 2 110 9 117 9.39e-46 144

Find 27 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTGGAGAAGTTTCTTC+AGG 0.314117 1.2:-66193983 MS.gene20740:CDS
GCCAGTTGAGGATAAGATTA+TGG 0.328511 1.2:-66194013 MS.gene20740:CDS
ATAGATATTGCGGTCCTTGT+TGG 0.377160 1.2:+66192951 None:intergenic
TCCATAATCTTATCCTCAAC+TGG 0.404476 1.2:+66194012 None:intergenic
CGGAGTGAAGGGTAAGAAGA+AGG 0.409364 1.2:-66194064 MS.gene20740:CDS
ATCGGAGGTAACAGTGATCT+TGG 0.422181 1.2:+66193894 None:intergenic
GATTATGGACATCGCTTCCC+TGG 0.434039 1.2:-66193998 MS.gene20740:CDS
CGCGAAAATGAGCGGAGTGA+AGG 0.462475 1.2:-66194076 None:intergenic
TCTCCTGAAGAAACTTCTCC+AGG 0.463361 1.2:+66193980 None:intergenic
AAAAGCACAATGTGAGGGAT+TGG 0.474548 1.2:-66192990 MS.gene20740:CDS
CTTCAACATTGCTGAGAATG+AGG 0.480373 1.2:-66192917 MS.gene20740:CDS
CCGATAGCAATTTCTCCAAA+AGG 0.482446 1.2:-66193876 MS.gene20740:intron
ATTAAAGTTGGTGGTAAAGC+TGG 0.482585 1.2:-66193955 MS.gene20740:CDS
GCGAAAATGAGCGGAGTGAA+GGG 0.509201 1.2:-66194075 None:intergenic
CTTCAGGAGAGGATTAAAGT+TGG 0.530224 1.2:-66193967 MS.gene20740:CDS
CAGGAGAGGATTAAAGTTGG+TGG 0.545199 1.2:-66193964 MS.gene20740:CDS
GGTGGTAAAGCTGGTGCTCT+CGG 0.555561 1.2:-66193946 MS.gene20740:CDS
CGATTGCGCGAAGCCAGTTG+AGG 0.561050 1.2:-66194025 MS.gene20740:CDS
TGGAGAAGTTTCTTCAGGAG+AGG 0.565476 1.2:-66193978 MS.gene20740:CDS
GGAGTGAAGGGTAAGAAGAA+GGG 0.572006 1.2:-66194063 MS.gene20740:CDS
ATCTCAACTCATAGATATTG+CGG 0.582807 1.2:+66192941 None:intergenic
AGTCTCAACGCGAAAATGAG+CGG 0.596462 1.2:-66194084 None:intergenic
TTTGGAGAAATTGCTATCGG+AGG 0.603460 1.2:+66193879 None:intergenic
TCGTGTTATTGCATCCAACA+AGG 0.613064 1.2:-66192965 MS.gene20740:CDS
TTCAACATTGCTGAGAATGA+GGG 0.613248 1.2:-66192916 MS.gene20740:CDS
CTCCTGAAGAAACTTCTCCA+GGG 0.621359 1.2:+66193981 None:intergenic
TTTGTCACGAGTAACAGTAA+CGG 0.687075 1.2:+66193918 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTATGTCTTAATTTTTTT+TGG - chr1.2:66193845-66193864 MS.gene20740:intron 15.0%
!! ATTTGACATTATTATGATTC+TGG - chr1.2:66193725-66193744 MS.gene20740:intron 20.0%
!!! TATTTGTTGATTAATGTGTT+AGG - chr1.2:66193132-66193151 MS.gene20740:intron 20.0%
! AAAAATAGTTAAACTGAGCT+GGG + chr1.2:66193776-66193795 None:intergenic 25.0%
! ATATGCTTCATGTAATTGTA+TGG - chr1.2:66193281-66193300 MS.gene20740:intron 25.0%
! ATATTTAGTGTATGTTTGGA+TGG - chr1.2:66193332-66193351 MS.gene20740:intron 25.0%
! TAAAAATAGTTAAACTGAGC+TGG + chr1.2:66193777-66193796 None:intergenic 25.0%
! TATGCTTCATGTAATTGTAT+GGG - chr1.2:66193282-66193301 MS.gene20740:intron 25.0%
! TATTTAGTGTATGTTTGGAT+GGG - chr1.2:66193333-66193352 MS.gene20740:intron 25.0%
! TCGTATATTTAGTGTATGTT+TGG - chr1.2:66193328-66193347 MS.gene20740:intron 25.0%
ATACAAGTCTTCTTCTTCTT+TGG - chr1.2:66193868-66193887 MS.gene20740:intron 30.0%
ATCTCAACTCATAGATATTG+CGG + chr1.2:66194031-66194050 None:intergenic 30.0%
CATGTGAACATTTCATTTAC+AGG + chr1.2:66193188-66193207 None:intergenic 30.0%
CATGTGTTTGAGTAATTATG+TGG - chr1.2:66193204-66193223 MS.gene20740:intron 30.0%
GTCAAATTCTATTCACATAC+AGG + chr1.2:66193712-66193731 None:intergenic 30.0%
! AAGATGAAGAAGTTACCTTT+TGG + chr1.2:66193111-66193130 None:intergenic 30.0%
! ACTGTAAGTCACCATAATTT+TGG + chr1.2:66193407-66193426 None:intergenic 30.0%
! CTGTAAGTCACCATAATTTT+GGG + chr1.2:66193406-66193425 None:intergenic 30.0%
!!! TTTTGTTATTTTCTACCGTG+AGG - chr1.2:66193514-66193533 MS.gene20740:intron 30.0%
ACAGGAATACTAGACAATAG+TGG + chr1.2:66193694-66193713 None:intergenic 35.0%
AGTTGACTACACACATCAAT+TGG + chr1.2:66193448-66193467 None:intergenic 35.0%
ATTGAATATTGAGACAGTGC+TGG - chr1.2:66193252-66193271 MS.gene20740:intron 35.0%
GGACAAAAATAATCACACGA+AGG + chr1.2:66193905-66193924 None:intergenic 35.0%
TCCATAATCTTATCCTCAAC+TGG + chr1.2:66192960-66192979 None:intergenic 35.0%
TGTCATGAGAATCCTTAAGT+TGG + chr1.2:66193623-66193642 None:intergenic 35.0%
TTCAACATTGCTGAGAATGA+GGG - chr1.2:66194053-66194072 MS.gene20740:CDS 35.0%
TTTGTCACGAGTAACAGTAA+CGG + chr1.2:66193054-66193073 None:intergenic 35.0%
! ACTTGAAAAAGCACAATGTG+AGG - chr1.2:66193973-66193992 MS.gene20740:CDS 35.0%
! ATGATTTGTGTAAATGGTCC+TGG - chr1.2:66193665-66193684 MS.gene20740:intron 35.0%
! CGTAGGTTTTCCCAAAATTA+TGG - chr1.2:66193393-66193412 MS.gene20740:intron 35.0%
! CTTGAAAAAGCACAATGTGA+GGG - chr1.2:66193974-66193993 MS.gene20740:CDS 35.0%
! GCGAGTATGATTTGTGTAAA+TGG - chr1.2:66193659-66193678 MS.gene20740:intron 35.0%
!! ATGTGGCTTTGAAATGTTGA+TGG - chr1.2:66193221-66193240 MS.gene20740:intron 35.0%
!! ATTAAAGTTGGTGGTAAAGC+TGG - chr1.2:66193014-66193033 MS.gene20740:CDS 35.0%
!! CCTTTTGGAGAAATTGCTAT+CGG + chr1.2:66193096-66193115 None:intergenic 35.0%
!!! GTGCTTTTTCAAGTACTTCT+TGG + chr1.2:66193967-66193986 None:intergenic 35.0%
AGGTCTAGTTGTTGCTGTAT+GGG - chr1.2:66193534-66193553 MS.gene20740:intron 40.0%
ATAGATATTGCGGTCCTTGT+TGG + chr1.2:66194021-66194040 None:intergenic 40.0%
CCGATAGCAATTTCTCCAAA+AGG - chr1.2:66193093-66193112 MS.gene20740:intron 40.0%
CTTCAACATTGCTGAGAATG+AGG - chr1.2:66194052-66194071 MS.gene20740:CDS 40.0%
CTTCAGGAGAGGATTAAAGT+TGG - chr1.2:66193002-66193021 MS.gene20740:CDS 40.0%
GAAAATGCCATTACCAGTCT+TGG - chr1.2:66193570-66193589 MS.gene20740:intron 40.0%
GCCAGTTGAGGATAAGATTA+TGG - chr1.2:66192956-66192975 MS.gene20740:CDS 40.0%
GGTCAAGTACTTCAAATACC+TGG + chr1.2:66193946-66193965 None:intergenic 40.0%
GTACTTCAAATACCTGGCAA+AGG + chr1.2:66193940-66193959 None:intergenic 40.0%
TAATCAACACTACCTTTGCC+AGG - chr1.2:66193925-66193944 MS.gene20740:CDS 40.0%
TGGGAAAACCTACGAATTCA+GGG + chr1.2:66193387-66193406 None:intergenic 40.0%
TTAGTGTATGTTTGGATGGG+TGG - chr1.2:66193336-66193355 MS.gene20740:intron 40.0%
TTGGGAAAACCTACGAATTC+AGG + chr1.2:66193388-66193407 None:intergenic 40.0%
TTTGGAGAAATTGCTATCGG+AGG + chr1.2:66193093-66193112 None:intergenic 40.0%
! AAAAGCACAATGTGAGGGAT+TGG - chr1.2:66193979-66193998 MS.gene20740:CDS 40.0%
! TCGTGTTATTGCATCCAACA+AGG - chr1.2:66194004-66194023 MS.gene20740:CDS 40.0%
!! TGAGAATCCTTAAGTTGGCA+CGG + chr1.2:66193618-66193637 None:intergenic 40.0%
!! TGGCAAAGGTAGTGTTGATT+AGG + chr1.2:66193926-66193945 None:intergenic 40.0%
ACAGCAACAACTAGACCTCA+CGG + chr1.2:66193532-66193551 None:intergenic 45.0%
ATCGGAGGTAACAGTGATCT+TGG + chr1.2:66193078-66193097 None:intergenic 45.0%
CAAACATCACAAGCCAAGAC+TGG + chr1.2:66193586-66193605 None:intergenic 45.0%
CAGGAGAGGATTAAAGTTGG+TGG - chr1.2:66193005-66193024 MS.gene20740:CDS 45.0%
CTCCTGAAGAAACTTCTCCA+GGG + chr1.2:66192991-66193010 None:intergenic 45.0%
GAGGTCTAGTTGTTGCTGTA+TGG - chr1.2:66193533-66193552 MS.gene20740:intron 45.0%
GGAGTGAAGGGTAAGAAGAA+GGG - chr1.2:66192906-66192925 MS.gene20740:CDS 45.0%
TCACAAGCCAAGACTGGTAA+TGG + chr1.2:66193580-66193599 None:intergenic 45.0%
TCTCCTGAAGAAACTTCTCC+AGG + chr1.2:66192992-66193011 None:intergenic 45.0%
TGGAGAAGTTTCTTCAGGAG+AGG - chr1.2:66192991-66193010 MS.gene20740:CDS 45.0%
TTCCCTGGAGAAGTTTCTTC+AGG - chr1.2:66192986-66193005 MS.gene20740:CDS 45.0%
TTCTGGTGCAGTTACTGAAC+TGG - chr1.2:66193742-66193761 MS.gene20740:intron 45.0%
!! ACTAGACAATAGTGGACACC+AGG + chr1.2:66193686-66193705 None:intergenic 45.0%
!! GAGAATCCTTAAGTTGGCAC+GGG + chr1.2:66193617-66193636 None:intergenic 45.0%
CGGAGTGAAGGGTAAGAAGA+AGG - chr1.2:66192905-66192924 MS.gene20740:CDS 50.0%
CGTTAGCACCCTGAATTCGT+AGG - chr1.2:66193376-66193395 MS.gene20740:intron 50.0%
GATTATGGACATCGCTTCCC+TGG - chr1.2:66192971-66192990 MS.gene20740:CDS 50.0%
TGCATTCCCGTGCCAACTTA+AGG - chr1.2:66193608-66193627 MS.gene20740:intron 50.0%
GGTGGTAAAGCTGGTGCTCT+CGG - chr1.2:66193023-66193042 MS.gene20740:CDS 55.0%
CGATTGCGCGAAGCCAGTTG+AGG - chr1.2:66192944-66192963 MS.gene20740:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66192900 66194091 66192900 ID=MS.gene20740
chr1.2 mRNA 66192900 66194091 66192900 ID=MS.gene20740.t1;Parent=MS.gene20740
chr1.2 exon 66193877 66194091 66193877 ID=MS.gene20740.t1.exon1;Parent=MS.gene20740.t1
chr1.2 CDS 66193877 66194091 66193877 ID=cds.MS.gene20740.t1;Parent=MS.gene20740.t1
chr1.2 exon 66192900 66193044 66192900 ID=MS.gene20740.t1.exon2;Parent=MS.gene20740.t1
chr1.2 CDS 66192900 66193044 66192900 ID=cds.MS.gene20740.t1;Parent=MS.gene20740.t1
Gene Sequence

>MS.gene20740

ATGAGCGGAGTGAAGGGTAAGAAGAAGGGAACAAGCTTCGTGATCGATTGCGCGAAGCCAGTTGAGGATAAGATTATGGACATCGCTTCCCTGGAGAAGTTTCTTCAGGAGAGGATTAAAGTTGGTGGTAAAGCTGGTGCTCTCGGTGATTCCGTTACTGTTACTCGTGACAAATCCAAGATCACTGTTACCTCCGATAGCAATTTCTCCAAAAGGTAACTTCTTCATCTTTTATTTGTTGATTAATGTGTTAGGTCAAATATTTGTAAGAGTTAGATCTAGTATCCTGTAAATGAAATGTTCACATGTGTTTGAGTAATTATGTGGCTTTGAAATGTTGATGGAGAATTCGATTGAATATTGAGACAGTGCTGGAATGAAATATGCTTCATGTAATTGTATGGGTTAGATTCTTTGAATTTAACTACTCGTATATTTAGTGTATGTTTGGATGGGTGGTGTGTTTGTCATAATCTCGTTAGCACCCTGAATTCGTAGGTTTTCCCAAAATTATGGTGACTTACAGTGATTTTGAAATTTCACTGCCAATTGATGTGTGTAGTCAACTCTTCAAAAGTGCACTTTTCAACATAAAATCAACATCTATGTGTTTCTTTTGTTATTTTCTACCGTGAGGTCTAGTTGTTGCTGTATGGGCACACTGATGTAAGAAAATGCCATTACCAGTCTTGGCTTGTGATGTTTGCATGCATTCCCGTGCCAACTTAAGGATTCTCATGACAATTTTTACATTATTGAGCGAGTATGATTTGTGTAAATGGTCCTGGTGTCCACTATTGTCTAGTATTCCTGTATGTGAATAGAATTTGACATTATTATGATTCTGGTGCAGTTACTGAACTGGATTCTTGTCCCAGCTCAGTTTAACTATTTTTAGTAACTGTTTGTTCAAATCTGTATGCAATCAATTGAAATCATTTTTTTCTTTATGTCTTAATTTTTTTTGGATACAAGTCTTCTTCTTCTTTGGTTTTTCTATTGCCTTCGTGTGATTATTTTTGTCCTAATCAACACTACCTTTGCCAGGTATTTGAAGTACTTGACCAAGAAGTACTTGAAAAAGCACAATGTGAGGGATTGGCTTCGTGTTATTGCATCCAACAAGGACCGCAATATCTATGAGTTGAGATACTTCAACATTGCTGAGAATGAGGGTGAAGATGAAGACTAA

Protein sequence

>MS.gene20740.t1

MSGVKGKKKGTSFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVTRDKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNIYELRYFNIAENEGEDED