Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064072.t1 | XP_003626648.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 6.30E-29 | 136 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064072.t1 | Q9C9Z5 | 77.4 | 62 | 14 | 0 | 1 | 62 | 1 | 62 | 2.0e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064072.t1 | Q1STQ6 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 4.6e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene064072 | 0.864984 | 8.20E-65 | -1.69E-46 |
| MS.gene049341 | MS.gene064072 | 0.849907 | 2.39E-60 | -1.69E-46 |
| MS.gene049382 | MS.gene064072 | 0.811014 | 8.65E-51 | -1.69E-46 |
| MS.gene049756 | MS.gene064072 | 0.837442 | 5.19E-57 | -1.69E-46 |
| MS.gene04984 | MS.gene064072 | 0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene064072 | 0.808575 | 2.90E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene064072 | 0.905564 | 3.76E-80 | -1.69E-46 |
| MS.gene050303 | MS.gene064072 | 0.810401 | 1.17E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene064072 | 0.809744 | 1.63E-50 | -1.69E-46 |
| MS.gene050320 | MS.gene064072 | 0.817493 | 3.20E-52 | -1.69E-46 |
| MS.gene050437 | MS.gene064072 | 0.866562 | 2.60E-65 | -1.69E-46 |
| MS.gene050689 | MS.gene064072 | 0.828907 | 6.93E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene064072 | 0.828624 | 8.10E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene064072 | 0.859014 | 5.54E-63 | -1.69E-46 |
| MS.gene050838 | MS.gene064072 | 0.842379 | 2.68E-58 | -1.69E-46 |
| MS.gene050955 | MS.gene064072 | 0.801986 | 6.95E-49 | -1.69E-46 |
| MS.gene051063 | MS.gene064072 | 0.852343 | 4.91E-61 | -1.69E-46 |
| MS.gene051241 | MS.gene064072 | 0.838271 | 3.18E-57 | -1.69E-46 |
| MS.gene051243 | MS.gene064072 | 0.850744 | 1.39E-60 | -1.69E-46 |
| MS.gene051245 | MS.gene064072 | 0.876907 | 9.61E-69 | -1.69E-46 |
| MS.gene051328 | MS.gene064072 | 0.859253 | 4.70E-63 | -1.69E-46 |
| MS.gene051330 | MS.gene064072 | 0.801397 | 9.18E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene064072 | 0.806932 | 6.47E-50 | -1.69E-46 |
| MS.gene05134 | MS.gene064072 | 0.850451 | 1.68E-60 | -1.69E-46 |
| MS.gene051451 | MS.gene064072 | 0.829636 | 4.61E-55 | -1.69E-46 |
| MS.gene051633 | MS.gene064072 | 0.839529 | 1.50E-57 | -1.69E-46 |
| MS.gene051746 | MS.gene064072 | 0.858406 | 8.42E-63 | -1.69E-46 |
| MS.gene051748 | MS.gene064072 | 0.856245 | 3.66E-62 | -1.69E-46 |
| MS.gene051756 | MS.gene064072 | 0.833068 | 6.59E-56 | -1.69E-46 |
| MS.gene051757 | MS.gene064072 | 0.858173 | 9.88E-63 | -1.69E-46 |
| MS.gene051789 | MS.gene064072 | 0.808707 | 2.71E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene064072 | 0.824395 | 8.26E-54 | -1.69E-46 |
| MS.gene051903 | MS.gene064072 | 0.863156 | 3.04E-64 | -1.69E-46 |
| MS.gene052133 | MS.gene064072 | 0.819773 | 9.74E-53 | -1.69E-46 |
| MS.gene052202 | MS.gene064072 | 0.810774 | 9.75E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene064072 | 0.860776 | 1.63E-63 | -1.69E-46 |
| MS.gene052302 | MS.gene064072 | 0.821101 | 4.83E-53 | -1.69E-46 |
| MS.gene052376 | MS.gene064072 | 0.815346 | 9.69E-52 | -1.69E-46 |
| MS.gene052856 | MS.gene064072 | 0.810946 | 8.95E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene064072 | 0.852801 | 3.64E-61 | -1.69E-46 |
| MS.gene053008 | MS.gene064072 | 0.809265 | 2.06E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene064072 | 0.8713 | 7.59E-67 | -1.69E-46 |
| MS.gene053216 | MS.gene064072 | 0.803949 | 2.73E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene064072 | 0.802177 | 6.35E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene064072 | 0.818097 | 2.34E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene064072 | 0.821747 | 3.43E-53 | -1.69E-46 |
| MS.gene055133 | MS.gene064072 | 0.834657 | 2.64E-56 | -1.69E-46 |
| MS.gene055162 | MS.gene064072 | 0.867494 | 1.31E-65 | -1.69E-46 |
| MS.gene055424 | MS.gene064072 | 0.807171 | 5.76E-50 | -1.69E-46 |
| MS.gene055441 | MS.gene064072 | 0.820855 | 5.50E-53 | -1.69E-46 |
| MS.gene055623 | MS.gene064072 | 0.810064 | 1.39E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene064072 | -0.814219 | 1.72E-51 | -1.69E-46 |
| MS.gene056432 | MS.gene064072 | 0.851022 | 1.16E-60 | -1.69E-46 |
| MS.gene056580 | MS.gene064072 | 0.83632 | 1.00E-56 | -1.69E-46 |
| MS.gene056601 | MS.gene064072 | 0.860684 | 1.74E-63 | -1.69E-46 |
| MS.gene056631 | MS.gene064072 | 0.879193 | 1.52E-69 | -1.69E-46 |
| MS.gene056760 | MS.gene064072 | 0.871307 | 7.55E-67 | -1.69E-46 |
| MS.gene056787 | MS.gene064072 | 0.801323 | 9.50E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene064072 | 0.883484 | 4.31E-71 | -1.69E-46 |
| MS.gene057094 | MS.gene064072 | 0.827194 | 1.79E-54 | -1.69E-46 |
| MS.gene057140 | MS.gene064072 | 0.873884 | 1.04E-67 | -1.69E-46 |
| MS.gene057234 | MS.gene064072 | 0.911047 | 9.46E-83 | -1.69E-46 |
| MS.gene057705 | MS.gene064072 | 0.825893 | 3.66E-54 | -1.69E-46 |
| MS.gene05795 | MS.gene064072 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene058054 | MS.gene064072 | 0.831864 | 1.31E-55 | -1.69E-46 |
| MS.gene058055 | MS.gene064072 | 0.88503 | 1.15E-71 | -1.69E-46 |
| MS.gene058084 | MS.gene064072 | 0.816559 | 5.20E-52 | -1.69E-46 |
| MS.gene05809 | MS.gene064072 | 0.817294 | 3.55E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene064072 | 0.826279 | 2.96E-54 | -1.69E-46 |
| MS.gene058238 | MS.gene064072 | 0.871273 | 7.75E-67 | -1.69E-46 |
| MS.gene058646 | MS.gene064072 | 0.818633 | 1.77E-52 | -1.69E-46 |
| MS.gene058654 | MS.gene064072 | 0.827436 | 1.57E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene064072 | 0.921295 | 4.30E-88 | -1.69E-46 |
| MS.gene059151 | MS.gene064072 | 0.801259 | 9.80E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene064072 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene064072 | 0.879109 | 1.63E-69 | -1.69E-46 |
| MS.gene06001 | MS.gene064072 | 0.871825 | 5.09E-67 | -1.69E-46 |
| MS.gene060165 | MS.gene064072 | 0.810664 | 1.03E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene064072 | 0.802929 | 4.44E-49 | -1.69E-46 |
| MS.gene060463 | MS.gene064072 | 0.825282 | 5.10E-54 | -1.69E-46 |
| MS.gene060465 | MS.gene064072 | 0.828548 | 8.46E-55 | -1.69E-46 |
| MS.gene060586 | MS.gene064072 | 0.802437 | 5.61E-49 | -1.69E-46 |
| MS.gene060702 | MS.gene064072 | 0.83552 | 1.60E-56 | -1.69E-46 |
| MS.gene061145 | MS.gene064072 | 0.845539 | 3.81E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064072.t1 | MTR_8g005360 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.66e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064072.t1 | AT3G08610 | 75.000 | 64 | 16 | 0 | 1 | 64 | 1 | 64 | 2.40e-33 | 108 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTACCTGTTTGTTAAT+TGG | 0.273208 | 8.2:-84150550 | None:intergenic |
| TGCTGGAATGCTTTGTGTTA+TGG | 0.308874 | 8.2:+84150313 | MS.gene064072:CDS |
| GCTGGAATGCTTTGTGTTAT+GGG | 0.333642 | 8.2:+84150314 | MS.gene064072:CDS |
| TTACCTTTGGGAATAATTGC+TGG | 0.341296 | 8.2:+84150296 | MS.gene064072:CDS |
| GTTGGAAGCTATGTTACCTT+TGG | 0.352532 | 8.2:+84150283 | MS.gene064072:CDS |
| TTGGAAGCTATGTTACCTTT+GGG | 0.406774 | 8.2:+84150284 | MS.gene064072:CDS |
| CTTCTTGTCCCTACGTTCCA+TGG | 0.446698 | 8.2:-84150505 | None:intergenic |
| AAATCATTGCCGATGTGCTT+AGG | 0.473520 | 8.2:-84150471 | None:intergenic |
| AGCACATCGGCAATGATTTG+TGG | 0.486432 | 8.2:+84150475 | MS.gene064072:CDS |
| AGAGATGGCGTTGCATTGGT+TGG | 0.487344 | 8.2:+84150265 | None:intergenic |
| GCACATCGGCAATGATTTGT+GGG | 0.508503 | 8.2:+84150476 | MS.gene064072:CDS |
| AGAGAGAGATGGCGTTGCAT+TGG | 0.509492 | 8.2:+84150261 | None:intergenic |
| CGGCAATGATTTGTGGGACG+TGG | 0.531999 | 8.2:+84150482 | MS.gene064072:CDS |
| TGATTTGTGGGACGTGGCCA+TGG | 0.553811 | 8.2:+84150488 | MS.gene064072:CDS |
| GGACGTGGCCATGGAACGTA+GGG | 0.570234 | 8.2:+84150497 | MS.gene064072:CDS |
| ATTCCAGCAATTATTCCCAA+AGG | 0.601922 | 8.2:-84150299 | None:intergenic |
| TGTGGTTAGCCTAAGCACAT+CGG | 0.628887 | 8.2:+84150462 | MS.gene064072:intron |
| CTCGTCCGTGAGCAGCTCTG+TGG | 0.632697 | 8.2:-84150355 | None:intergenic |
| TACATCCACAGAGCTGCTCA+CGG | 0.640107 | 8.2:+84150350 | MS.gene064072:CDS |
| GGGACGTGGCCATGGAACGT+AGG | 0.664354 | 8.2:+84150496 | MS.gene064072:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAAATTGAATTGAAT+TGG | + | chr8.2:84150430-84150449 | MS.gene064072:intron | 10.0% |
| !!! | TTGAATTGGATCTATTTTTG+TGG | + | chr8.2:84150444-84150463 | MS.gene064072:intron | 25.0% |
| ATTCCAGCAATTATTCCCAA+AGG | - | chr8.2:84150302-84150321 | None:intergenic | 35.0% | |
| TTGGAAGCTATGTTACCTTT+GGG | + | chr8.2:84150284-84150303 | MS.gene064072:CDS | 35.0% | |
| ! | TTACCTTTGGGAATAATTGC+TGG | + | chr8.2:84150296-84150315 | MS.gene064072:CDS | 35.0% |
| AAATCATTGCCGATGTGCTT+AGG | - | chr8.2:84150474-84150493 | None:intergenic | 40.0% | |
| GCTGGAATGCTTTGTGTTAT+GGG | + | chr8.2:84150314-84150333 | MS.gene064072:CDS | 40.0% | |
| GTTGGAAGCTATGTTACCTT+TGG | + | chr8.2:84150283-84150302 | MS.gene064072:CDS | 40.0% | |
| TGCTGGAATGCTTTGTGTTA+TGG | + | chr8.2:84150313-84150332 | MS.gene064072:CDS | 40.0% | |
| AGCACATCGGCAATGATTTG+TGG | + | chr8.2:84150475-84150494 | MS.gene064072:CDS | 45.0% | |
| ! | GCACATCGGCAATGATTTGT+GGG | + | chr8.2:84150476-84150495 | MS.gene064072:CDS | 45.0% |
| !! | TGTGGTTAGCCTAAGCACAT+CGG | + | chr8.2:84150462-84150481 | MS.gene064072:intron | 45.0% |
| CTTCTTGTCCCTACGTTCCA+TGG | - | chr8.2:84150508-84150527 | None:intergenic | 50.0% | |
| TACATCCACAGAGCTGCTCA+CGG | + | chr8.2:84150350-84150369 | MS.gene064072:CDS | 50.0% | |
| ! | CGGCAATGATTTGTGGGACG+TGG | + | chr8.2:84150482-84150501 | MS.gene064072:CDS | 55.0% |
| ! | TGATTTGTGGGACGTGGCCA+TGG | + | chr8.2:84150488-84150507 | MS.gene064072:CDS | 55.0% |
| GGACGTGGCCATGGAACGTA+GGG | + | chr8.2:84150497-84150516 | MS.gene064072:CDS | 60.0% | |
| CTCGTCCGTGAGCAGCTCTG+TGG | - | chr8.2:84150358-84150377 | None:intergenic | 65.0% | |
| GGGACGTGGCCATGGAACGT+AGG | + | chr8.2:84150496-84150515 | MS.gene064072:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 84150269 | 84150557 | 84150269 | ID=MS.gene064072 |
| chr8.2 | mRNA | 84150269 | 84150557 | 84150269 | ID=MS.gene064072.t1;Parent=MS.gene064072 |
| chr8.2 | exon | 84150269 | 84150376 | 84150269 | ID=MS.gene064072.t1.exon1;Parent=MS.gene064072.t1 |
| chr8.2 | CDS | 84150269 | 84150376 | 84150269 | ID=cds.MS.gene064072.t1;Parent=MS.gene064072.t1 |
| chr8.2 | exon | 84150471 | 84150557 | 84150471 | ID=MS.gene064072.t1.exon2;Parent=MS.gene064072.t1 |
| chr8.2 | CDS | 84150471 | 84150557 | 84150471 | ID=cds.MS.gene064072.t1;Parent=MS.gene064072.t1 |
| Gene Sequence |
| Protein sequence |
