Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07042.t1 | XP_003625958.1 | 94.9 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 9.10E-103 | 382.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07042.t1 | G7KSS5 | 94.9 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 6.6e-103 | 382.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene07042 | -0.830016 | 3.72E-55 | -1.69E-46 |
| MS.gene04921 | MS.gene07042 | 0.830714 | 2.51E-55 | -1.69E-46 |
| MS.gene049633 | MS.gene07042 | -0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene07042 | -0.85646 | 3.17E-62 | -1.69E-46 |
| MS.gene050323 | MS.gene07042 | -0.813011 | 3.18E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene07042 | -0.831088 | 2.04E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene07042 | -0.836862 | 7.30E-57 | -1.69E-46 |
| MS.gene050577 | MS.gene07042 | -0.842013 | 3.35E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene07042 | -0.828259 | 9.93E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene07042 | -0.822512 | 2.28E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene07042 | -0.820455 | 6.80E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene07042 | -0.841549 | 4.44E-58 | -1.69E-46 |
| MS.gene051044 | MS.gene07042 | 0.808632 | 2.82E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene07042 | 0.819881 | 9.20E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene07042 | 0.826607 | 2.47E-54 | -1.69E-46 |
| MS.gene051317 | MS.gene07042 | -0.822915 | 1.83E-53 | -1.69E-46 |
| MS.gene051364 | MS.gene07042 | 0.808159 | 3.55E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene07042 | 0.80307 | 4.16E-49 | -1.69E-46 |
| MS.gene051422 | MS.gene07042 | 0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene051440 | MS.gene07042 | 0.814139 | 1.79E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene07042 | 0.864667 | 1.03E-64 | -1.69E-46 |
| MS.gene051973 | MS.gene07042 | 0.81067 | 1.03E-50 | -1.69E-46 |
| MS.gene052015 | MS.gene07042 | 0.824895 | 6.30E-54 | -1.69E-46 |
| MS.gene052486 | MS.gene07042 | -0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene052494 | MS.gene07042 | 0.854732 | 1.01E-61 | -1.69E-46 |
| MS.gene052606 | MS.gene07042 | 0.834189 | 3.46E-56 | -1.69E-46 |
| MS.gene053097 | MS.gene07042 | 0.840362 | 9.10E-58 | -1.69E-46 |
| MS.gene053203 | MS.gene07042 | -0.816148 | 6.42E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene07042 | 0.806246 | 9.03E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene07042 | -0.812919 | 3.33E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene07042 | 0.803746 | 3.01E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene07042 | 0.805785 | 1.13E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene07042 | -0.850431 | 1.71E-60 | -1.69E-46 |
| MS.gene055251 | MS.gene07042 | 0.80264 | 5.10E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene07042 | -0.827164 | 1.82E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene07042 | -0.811731 | 6.05E-51 | -1.69E-46 |
| MS.gene055731 | MS.gene07042 | 0.802885 | 4.54E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene07042 | -0.827114 | 1.87E-54 | -1.69E-46 |
| MS.gene055750 | MS.gene07042 | -0.813269 | 2.79E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene07042 | -0.830015 | 3.73E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene07042 | -0.880251 | 6.40E-70 | -1.69E-46 |
| MS.gene056198 | MS.gene07042 | 0.80252 | 5.40E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene07042 | 0.805561 | 1.26E-49 | -1.69E-46 |
| MS.gene056266 | MS.gene07042 | -0.811074 | 8.40E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene07042 | -0.857069 | 2.10E-62 | -1.69E-46 |
| MS.gene056694 | MS.gene07042 | 0.843585 | 1.28E-58 | -1.69E-46 |
| MS.gene056748 | MS.gene07042 | 0.812811 | 3.51E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene07042 | 0.812633 | 3.84E-51 | -1.69E-46 |
| MS.gene056988 | MS.gene07042 | 0.810547 | 1.09E-50 | -1.69E-46 |
| MS.gene057499 | MS.gene07042 | 0.800246 | 1.58E-48 | -1.69E-46 |
| MS.gene057768 | MS.gene07042 | 0.864404 | 1.25E-64 | -1.69E-46 |
| MS.gene058111 | MS.gene07042 | 0.840368 | 9.07E-58 | -1.69E-46 |
| MS.gene058171 | MS.gene07042 | 0.84465 | 6.63E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene07042 | 0.837501 | 5.01E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene07042 | 0.847682 | 9.91E-60 | -1.69E-46 |
| MS.gene058334 | MS.gene07042 | 0.835185 | 1.94E-56 | -1.69E-46 |
| MS.gene05837 | MS.gene07042 | -0.830199 | 3.36E-55 | -1.69E-46 |
| MS.gene058382 | MS.gene07042 | 0.803442 | 3.48E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene07042 | -0.81176 | 5.96E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene07042 | 0.802329 | 5.91E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene07042 | -0.807239 | 5.57E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene07042 | -0.816805 | 4.58E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene07042 | -0.82122 | 4.53E-53 | -1.69E-46 |
| MS.gene05960 | MS.gene07042 | 0.825931 | 3.58E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene07042 | -0.826406 | 2.76E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene07042 | -0.83907 | 1.97E-57 | -1.69E-46 |
| MS.gene060076 | MS.gene07042 | 0.803239 | 3.83E-49 | -1.69E-46 |
| MS.gene060187 | MS.gene07042 | 0.805584 | 1.24E-49 | -1.69E-46 |
| MS.gene06027 | MS.gene07042 | -0.803304 | 3.72E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene07042 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene06042 | MS.gene07042 | 0.81197 | 5.36E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene07042 | 0.812541 | 4.03E-51 | -1.69E-46 |
| MS.gene060595 | MS.gene07042 | 0.835539 | 1.58E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene07042 | -0.81923 | 1.30E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene07042 | -0.839803 | 1.27E-57 | -1.69E-46 |
| MS.gene060823 | MS.gene07042 | 0.828231 | 1.01E-54 | -1.69E-46 |
| MS.gene06084 | MS.gene07042 | 0.819932 | 8.96E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene07042 | -0.851825 | 6.89E-61 | -1.69E-46 |
| MS.gene061044 | MS.gene07042 | 0.8955 | 9.03E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07042.t1 | MTR_7g109410 | 94.924 | 197 | 10 | 0 | 1 | 197 | 1 | 197 | 1.96e-141 | 392 |
| MS.gene07042.t1 | MTR_1g087230 | 56.853 | 197 | 75 | 5 | 1 | 192 | 1 | 192 | 4.54e-72 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07042.t1 | AT4G02090 | 38.579 | 197 | 83 | 9 | 9 | 183 | 16 | 196 | 4.17e-28 | 105 |
Find 39 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTTTCCTCCATCATTTC+TGG | 0.096966 | 7.2:+5545400 | None:intergenic |
| ATAAGCTCTGCTACTGTTAT+TGG | 0.153548 | 7.2:+5545760 | None:intergenic |
| ACCATCATAGTAAATGATTT+TGG | 0.206418 | 7.2:+5545804 | None:intergenic |
| AGAGCTCGATAGATAATTAT+TGG | 0.225759 | 7.2:+5545540 | None:intergenic |
| AACTCAACAACAACATGTTT+TGG | 0.228014 | 7.2:+5545727 | None:intergenic |
| TTGTACCAATCAAACTTCTA+AGG | 0.282032 | 7.2:+5545272 | None:intergenic |
| AATTTGAGTAGGCAATTATC+AGG | 0.284221 | 7.2:-5545367 | MS.gene07042:CDS |
| GCCTAGCTTGGATACTATTA+AGG | 0.302250 | 7.2:-5545332 | MS.gene07042:CDS |
| GCTCTCAAGGCTTTGTTCCT+TGG | 0.316149 | 7.2:-5545522 | MS.gene07042:CDS |
| GCTTTGTTCCTTGGCTTGTA+AGG | 0.325698 | 7.2:-5545513 | MS.gene07042:CDS |
| ACGGGCTTCCCTGGTTTCAT+TGG | 0.343712 | 7.2:+5545622 | None:intergenic |
| ACCAAAATCATTTACTATGA+TGG | 0.388071 | 7.2:-5545805 | MS.gene07042:CDS |
| AAGAATTCGACGACAATCTT+CGG | 0.416240 | 7.2:+5545588 | None:intergenic |
| ATTTCTAGGTTCTTGTCAGC+TGG | 0.436018 | 7.2:+5545667 | None:intergenic |
| TTGTGGCATGAAATGAGATA+TGG | 0.438798 | 7.2:+5545827 | None:intergenic |
| CTTAATCCGACGGGCTTCCC+TGG | 0.445311 | 7.2:+5545613 | None:intergenic |
| AGGTTCTTGTCAGCTGGTAG+TGG | 0.455725 | 7.2:+5545673 | None:intergenic |
| TTGTCAGCTGGTAGTGGTGT+TGG | 0.459537 | 7.2:+5545679 | None:intergenic |
| GTATTCCTTAGAAGTTTGAT+TGG | 0.462601 | 7.2:-5545277 | MS.gene07042:CDS |
| AAGGTTAAGAGAAAGATATC+TGG | 0.480587 | 7.2:-5545304 | MS.gene07042:CDS |
| GGATACTATTAAGGAAAAGA+AGG | 0.488586 | 7.2:-5545323 | MS.gene07042:CDS |
| CAGAGTATTTGAATTTGAGT+AGG | 0.490159 | 7.2:-5545378 | MS.gene07042:CDS |
| GAAAGGGTGGAAGCCTAGCT+TGG | 0.506451 | 7.2:-5545344 | MS.gene07042:CDS |
| TAAGAGAAAGATATCTGGTT+GGG | 0.509583 | 7.2:-5545299 | MS.gene07042:CDS |
| AGTAGGCAATTATCAGGGAA+AGG | 0.530002 | 7.2:-5545361 | MS.gene07042:CDS |
| TGCAGATAAATGAAGAAGAG+AGG | 0.538383 | 7.2:-5545444 | MS.gene07042:CDS |
| TCCTTAATAGTATCCAAGCT+AGG | 0.540949 | 7.2:+5545331 | None:intergenic |
| GGCAATTATCAGGGAAAGGG+TGG | 0.561848 | 7.2:-5545357 | MS.gene07042:CDS |
| AATTATCTATCGAGCTCTCA+AGG | 0.562278 | 7.2:-5545535 | MS.gene07042:CDS |
| AAAGAAAGTTGAAATTGGAG+AGG | 0.565965 | 7.2:-5545479 | MS.gene07042:CDS |
| GTCATGGTTCCAATGAAACC+AGG | 0.568862 | 7.2:-5545631 | MS.gene07042:CDS |
| TTAAGAGAAAGATATCTGGT+TGG | 0.583864 | 7.2:-5545300 | MS.gene07042:CDS |
| ATCTTCGGCACTTAATCCGA+CGG | 0.584491 | 7.2:+5545603 | None:intergenic |
| GTAGGCAATTATCAGGGAAA+GGG | 0.585684 | 7.2:-5545360 | MS.gene07042:CDS |
| ATTAAAAGATGAATTAACGA+TGG | 0.589712 | 7.2:+5545564 | None:intergenic |
| TCTTCGGCACTTAATCCGAC+GGG | 0.598400 | 7.2:+5545604 | None:intergenic |
| ATTTGAGTAGGCAATTATCA+GGG | 0.600835 | 7.2:-5545366 | MS.gene07042:CDS |
| TCATGGTTCCAATGAAACCA+GGG | 0.644756 | 7.2:-5545630 | MS.gene07042:CDS |
| ATGAAACCAGGGAAGCCCGT+CGG | 0.678146 | 7.2:-5545619 | MS.gene07042:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAAGAAAGTTGAAAT+TGG | - | chr7.2:5545615-5545634 | MS.gene07042:CDS | 20.0% |
| !! | AATGAACAAAAAATATGTCA+TGG | - | chr7.2:5545452-5545471 | MS.gene07042:CDS | 20.0% |
| !! | ATTAAAAGATGAATTAACGA+TGG | + | chr7.2:5545538-5545557 | None:intergenic | 20.0% |
| !!! | CATATTTTTTGTTCATTTCT+AGG | + | chr7.2:5545449-5545468 | None:intergenic | 20.0% |
| ! | ACCAAAATCATTTACTATGA+TGG | - | chr7.2:5545294-5545313 | MS.gene07042:CDS | 25.0% |
| !! | ACCATCATAGTAAATGATTT+TGG | + | chr7.2:5545298-5545317 | None:intergenic | 25.0% |
| !!! | ATAGTAAATGATTTTGGTTG+TGG | + | chr7.2:5545292-5545311 | None:intergenic | 25.0% |
| AAAGAAAGTTGAAATTGGAG+AGG | - | chr7.2:5545620-5545639 | MS.gene07042:CDS | 30.0% | |
| AAGGTTAAGAGAAAGATATC+TGG | - | chr7.2:5545795-5545814 | MS.gene07042:CDS | 30.0% | |
| AATTTGAGTAGGCAATTATC+AGG | - | chr7.2:5545732-5545751 | MS.gene07042:CDS | 30.0% | |
| AGAGCTCGATAGATAATTAT+TGG | + | chr7.2:5545562-5545581 | None:intergenic | 30.0% | |
| ATTTGAGTAGGCAATTATCA+GGG | - | chr7.2:5545733-5545752 | MS.gene07042:CDS | 30.0% | |
| GGATACTATTAAGGAAAAGA+AGG | - | chr7.2:5545776-5545795 | MS.gene07042:CDS | 30.0% | |
| GTATTCCTTAGAAGTTTGAT+TGG | - | chr7.2:5545822-5545841 | MS.gene07042:CDS | 30.0% | |
| TAAGAGAAAGATATCTGGTT+GGG | - | chr7.2:5545800-5545819 | MS.gene07042:CDS | 30.0% | |
| TTAAGAGAAAGATATCTGGT+TGG | - | chr7.2:5545799-5545818 | MS.gene07042:CDS | 30.0% | |
| TTGTACCAATCAAACTTCTA+AGG | + | chr7.2:5545830-5545849 | None:intergenic | 30.0% | |
| ! | AACTCAACAACAACATGTTT+TGG | + | chr7.2:5545375-5545394 | None:intergenic | 30.0% |
| ! | ACTCAACAACAACATGTTTT+GGG | + | chr7.2:5545374-5545393 | None:intergenic | 30.0% |
| ! | CAGAGTATTTGAATTTGAGT+AGG | - | chr7.2:5545721-5545740 | MS.gene07042:CDS | 30.0% |
| AAGAATTCGACGACAATCTT+CGG | + | chr7.2:5545514-5545533 | None:intergenic | 35.0% | |
| AATTATCTATCGAGCTCTCA+AGG | - | chr7.2:5545564-5545583 | MS.gene07042:CDS | 35.0% | |
| ATAAGCTCTGCTACTGTTAT+TGG | + | chr7.2:5545342-5545361 | None:intergenic | 35.0% | |
| TCCTTAATAGTATCCAAGCT+AGG | + | chr7.2:5545771-5545790 | None:intergenic | 35.0% | |
| TGCAGATAAATGAAGAAGAG+AGG | - | chr7.2:5545655-5545674 | MS.gene07042:CDS | 35.0% | |
| TTCAAAAACCTTACAAGCCA+AGG | + | chr7.2:5545597-5545616 | None:intergenic | 35.0% | |
| TTGTGGCATGAAATGAGATA+TGG | + | chr7.2:5545275-5545294 | None:intergenic | 35.0% | |
| ! | TGAGTTTTTGCCAGAAATGA+TGG | - | chr7.2:5545689-5545708 | MS.gene07042:CDS | 35.0% |
| AGTAGGCAATTATCAGGGAA+AGG | - | chr7.2:5545738-5545757 | MS.gene07042:CDS | 40.0% | |
| GCCTAGCTTGGATACTATTA+AGG | - | chr7.2:5545767-5545786 | MS.gene07042:CDS | 40.0% | |
| GTAGGCAATTATCAGGGAAA+GGG | - | chr7.2:5545739-5545758 | MS.gene07042:CDS | 40.0% | |
| TCATGGTTCCAATGAAACCA+GGG | - | chr7.2:5545469-5545488 | MS.gene07042:CDS | 40.0% | |
| ! | ATTTCTAGGTTCTTGTCAGC+TGG | + | chr7.2:5545435-5545454 | None:intergenic | 40.0% |
| ! | GTTTTTGCCAGAAATGATGG+AGG | - | chr7.2:5545692-5545711 | MS.gene07042:CDS | 40.0% |
| !!! | GTAGTGGTGTTGGTTTTTTC+TGG | + | chr7.2:5545413-5545432 | None:intergenic | 40.0% |
| ATCTTCGGCACTTAATCCGA+CGG | + | chr7.2:5545499-5545518 | None:intergenic | 45.0% | |
| GCTCTTTCCTCCATCATTTC+TGG | + | chr7.2:5545702-5545721 | None:intergenic | 45.0% | |
| GTCATGGTTCCAATGAAACC+AGG | - | chr7.2:5545468-5545487 | MS.gene07042:CDS | 45.0% | |
| !! | GCTTTGTTCCTTGGCTTGTA+AGG | - | chr7.2:5545586-5545605 | MS.gene07042:CDS | 45.0% |
| AGGTTCTTGTCAGCTGGTAG+TGG | + | chr7.2:5545429-5545448 | None:intergenic | 50.0% | |
| GGCAATTATCAGGGAAAGGG+TGG | - | chr7.2:5545742-5545761 | MS.gene07042:CDS | 50.0% | |
| TCTTCGGCACTTAATCCGAC+GGG | + | chr7.2:5545498-5545517 | None:intergenic | 50.0% | |
| !! | GCTCTCAAGGCTTTGTTCCT+TGG | - | chr7.2:5545577-5545596 | MS.gene07042:CDS | 50.0% |
| !! | TTGTCAGCTGGTAGTGGTGT+TGG | + | chr7.2:5545423-5545442 | None:intergenic | 50.0% |
| ACGGGCTTCCCTGGTTTCAT+TGG | + | chr7.2:5545480-5545499 | None:intergenic | 55.0% | |
| ATGAAACCAGGGAAGCCCGT+CGG | - | chr7.2:5545480-5545499 | MS.gene07042:CDS | 55.0% | |
| GAAAGGGTGGAAGCCTAGCT+TGG | - | chr7.2:5545755-5545774 | MS.gene07042:CDS | 55.0% | |
| ! | CTTAATCCGACGGGCTTCCC+TGG | + | chr7.2:5545489-5545508 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 5545264 | 5545857 | 5545264 | ID=MS.gene07042 |
| chr7.2 | mRNA | 5545264 | 5545857 | 5545264 | ID=MS.gene07042.t1;Parent=MS.gene07042 |
| chr7.2 | exon | 5545264 | 5545857 | 5545264 | ID=MS.gene07042.t1.exon1;Parent=MS.gene07042.t1 |
| chr7.2 | CDS | 5545264 | 5545857 | 5545264 | ID=cds.MS.gene07042.t1;Parent=MS.gene07042.t1 |
| Gene Sequence |
| Protein sequence |
