AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39496


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39496.t1 MTR_4g088515 92.164 268 7 1 1 254 1 268 1.28e-178 505
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39496.t1 AT4G26415 47.727 220 62 9 3 178 7 217 3.52e-45 154

Find 59 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCCTCAAGAACTCTT+TGG 0.228130 4.2:+22656108 MS.gene39496:CDS
TCAATGAATATAGTGGAATA+AGG 0.272127 4.2:+22656377 MS.gene39496:CDS
TGGTGTTGTGGCAAAGTTAA+TGG 0.280238 4.2:+22656633 MS.gene39496:CDS
GCCATTTGAGAGGCATGATT+TGG 0.298125 4.2:+22656753 MS.gene39496:CDS
TCCATGTATAGGTCATCTTT+TGG 0.313265 4.2:+22656493 MS.gene39496:CDS
TCTTATGCCTTTCTTCATCT+TGG 0.319608 4.2:-22656134 None:intergenic
TTTCTCCTCAAGAACTCTTT+GGG 0.349291 4.2:+22656109 MS.gene39496:CDS
AACCAACAAAATAGTACTAT+TGG 0.366556 4.2:+22656193 MS.gene39496:CDS
TGGATATTGTATTATGAAAC+CGG 0.367697 4.2:+22656858 MS.gene39496:CDS
GGATGAGTAGTGTTGATTGT+AGG 0.385703 4.2:+22656710 MS.gene39496:CDS
CAATGAATATAGTGGAATAA+GGG 0.392950 4.2:+22656378 MS.gene39496:CDS
AGAACCAAAGCTTGATGAAA+AGG 0.407802 4.2:+22656558 MS.gene39496:CDS
GCCATAGCTTCTAGAGAAAC+CGG 0.424661 4.2:-22656877 None:intergenic
GGTGTTGTGGCAAAGTTAAT+GGG 0.432813 4.2:+22656634 MS.gene39496:CDS
TGTGGCAAAGTTAATGGGTT+TGG 0.433784 4.2:+22656639 MS.gene39496:CDS
TCTTATACTACAATTAGAAA+TGG 0.443850 4.2:+22656327 MS.gene39496:CDS
CTCCTTGGTTGCCAACTACT+AGG 0.453422 4.2:-22656907 None:intergenic
GTTAGAAGAAGAAATAGGTC+TGG 0.472977 4.2:+22656820 MS.gene39496:CDS
AGTATAAGATCTTCTAATGT+TGG 0.477524 4.2:-22656313 None:intergenic
TCCAAATCATGCCTCTCAAA+TGG 0.478781 4.2:-22656754 None:intergenic
TGCTGCTTGTGATGTCAACT+TGG 0.485632 4.2:-22656237 None:intergenic
AGTACTATTGGCAATGGCAT+TGG 0.489373 4.2:+22656205 MS.gene39496:CDS
AAAACTAGCAATGGACATGC+AGG 0.491726 4.2:+22656780 MS.gene39496:CDS
CCCAAAAGATGACCTATACA+TGG 0.498041 4.2:-22656494 None:intergenic
CTAATCTAAACATATCTCCT+TGG 0.500292 4.2:-22656922 None:intergenic
TTGTGATGTCAACTTGGCTA+TGG 0.503039 4.2:-22656231 None:intergenic
TTCTCCCCAGCAACTGTTGG+TGG 0.503702 4.2:-22656592 None:intergenic
GTAGGAGACAAAATATAAGA+AGG 0.511223 4.2:+22656728 MS.gene39496:CDS
ATGGAAGAGATTCCATGTAT+AGG 0.512387 4.2:+22656482 MS.gene39496:CDS
ATTAACTTTGCCACAACACC+AGG 0.513967 4.2:-22656631 None:intergenic
TGGCAGGAGGTCCTAGTAGT+TGG 0.521625 4.2:+22656896 MS.gene39496:CDS
ACCGGTTTCTCTAGAAGCTA+TGG 0.522607 4.2:+22656876 MS.gene39496:CDS
TTGCTGGGGAGAACGTGGTT+TGG 0.527912 4.2:+22656602 MS.gene39496:CDS
ACATTGTTAGAAGAAGAAAT+AGG 0.529346 4.2:+22656815 MS.gene39496:CDS
ATGCTTTGAATCAATATCCA+AGG 0.531501 4.2:+22656449 MS.gene39496:CDS
AAATGGAGGAAGAGATGGCA+AGG 0.533678 4.2:+22656344 MS.gene39496:CDS
ATTAGAAATGGAGGAAGAGA+TGG 0.541356 4.2:+22656339 MS.gene39496:CDS
GAGTTCTTGAGGAGAAAGAA+TGG 0.549925 4.2:-22656103 None:intergenic
TTGGGAGCCAAGATGAAGAA+AGG 0.561593 4.2:+22656127 MS.gene39496:CDS
CAAAATAGTACTATTGGCAA+TGG 0.563916 4.2:+22656199 MS.gene39496:CDS
CTTCCATCAAGTGAAAACCT+TGG 0.575355 4.2:-22656466 None:intergenic
GTCCTAGTAGTTGGCAACCA+AGG 0.577078 4.2:+22656905 MS.gene39496:CDS
AATGAATATAGTGGAATAAG+GGG 0.579962 4.2:+22656379 MS.gene39496:CDS
TATACTACAATTAGAAATGG+AGG 0.596710 4.2:+22656330 MS.gene39496:CDS
TGGCTCCCAAAGAGTTCTTG+AGG 0.597986 4.2:-22656114 None:intergenic
GAAGCTATTCCAGTGTCAGT+AGG 0.605402 4.2:+22656661 MS.gene39496:CDS
TTTGGAGAGAAAACTAGCAA+TGG 0.605568 4.2:+22656771 MS.gene39496:CDS
AACGTGGTTTGGTGTAAACC+TGG 0.608504 4.2:+22656613 MS.gene39496:CDS
GTTTCTCTAGAAGCTATGGC+AGG 0.610709 4.2:+22656880 MS.gene39496:CDS
AACAGTTGCTGGGGAGAACG+TGG 0.612915 4.2:+22656597 MS.gene39496:CDS
AATATAGTGGAATAAGGGGA+AGG 0.620558 4.2:+22656383 MS.gene39496:CDS
GGAGACAAAATATAAGAAGG+AGG 0.626425 4.2:+22656731 MS.gene39496:CDS
TAAGAAGGAGGCCATTTGAG+AGG 0.627512 4.2:+22656743 MS.gene39496:CDS
CTGACATATTAAGATCTGCA+AGG 0.639107 4.2:+22656425 MS.gene39496:CDS
AGTTGACATCACAAGCAGCA+AGG 0.656859 4.2:+22656240 MS.gene39496:CDS
ACGTTCTCCCCAGCAACTGT+TGG 0.657823 4.2:-22656595 None:intergenic
TCTCTAGAAGCTATGGCAGG+AGG 0.665560 4.2:+22656883 MS.gene39496:CDS
TTGGTGTAAACCTGGTGTTG+TGG 0.669247 4.2:+22656621 MS.gene39496:CDS
AAACTAGCAATGGACATGCA+GGG 0.676573 4.2:+22656781 MS.gene39496:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTATACTACAATTAGAAA+TGG + chr4.2:22656327-22656346 MS.gene39496:CDS 20.0%
! AACCAACAAAATAGTACTAT+TGG + chr4.2:22656193-22656212 MS.gene39496:CDS 25.0%
! ACATTGTTAGAAGAAGAAAT+AGG + chr4.2:22656815-22656834 MS.gene39496:CDS 25.0%
! AGTATAAGATCTTCTAATGT+TGG - chr4.2:22656316-22656335 None:intergenic 25.0%
! GAAAAACTCAATGAATATAG+TGG + chr4.2:22656370-22656389 MS.gene39496:CDS 25.0%
! TATACTACAATTAGAAATGG+AGG + chr4.2:22656330-22656349 MS.gene39496:CDS 25.0%
! TGGATATTGTATTATGAAAC+CGG + chr4.2:22656858-22656877 MS.gene39496:CDS 25.0%
!! AATGAATATAGTGGAATAAG+GGG + chr4.2:22656379-22656398 MS.gene39496:CDS 25.0%
!! CAATGAATATAGTGGAATAA+GGG + chr4.2:22656378-22656397 MS.gene39496:CDS 25.0%
!! TCAATGAATATAGTGGAATA+AGG + chr4.2:22656377-22656396 MS.gene39496:CDS 25.0%
AAAGATCTGACAAAAAAGAG+AGG + chr4.2:22656689-22656708 MS.gene39496:CDS 30.0%
AGTTGTCACATTGCAAAAAA+GGG - chr4.2:22656273-22656292 None:intergenic 30.0%
CAAAATAGTACTATTGGCAA+TGG + chr4.2:22656199-22656218 MS.gene39496:CDS 30.0%
GTAGGAGACAAAATATAAGA+AGG + chr4.2:22656728-22656747 MS.gene39496:CDS 30.0%
! ATAACCTTTTCATCAAGCTT+TGG - chr4.2:22656565-22656584 None:intergenic 30.0%
! ATGCTTTGAATCAATATCCA+AGG + chr4.2:22656449-22656468 MS.gene39496:CDS 30.0%
! TGCCAATAGTACTATTTTGT+TGG - chr4.2:22656198-22656217 None:intergenic 30.0%
!!! ATTTTGTTGGTTTAGAGTTG+AGG - chr4.2:22656185-22656204 None:intergenic 30.0%
!!! TCTGGTTTTTGCTCTAAAAA+TGG + chr4.2:22656838-22656857 MS.gene39496:CDS 30.0%
AGAACCAAAGCTTGATGAAA+AGG + chr4.2:22656558-22656577 MS.gene39496:CDS 35.0%
ATGGAAGAGATTCCATGTAT+AGG + chr4.2:22656482-22656501 MS.gene39496:CDS 35.0%
ATTAGAAATGGAGGAAGAGA+TGG + chr4.2:22656339-22656358 MS.gene39496:CDS 35.0%
CTGACATATTAAGATCTGCA+AGG + chr4.2:22656425-22656444 MS.gene39496:CDS 35.0%
GAGTTGTCACATTGCAAAAA+AGG - chr4.2:22656274-22656293 None:intergenic 35.0%
GGAGACAAAATATAAGAAGG+AGG + chr4.2:22656731-22656750 MS.gene39496:CDS 35.0%
GTTAGAAGAAGAAATAGGTC+TGG + chr4.2:22656820-22656839 MS.gene39496:CDS 35.0%
GTTGTCACATTGCAAAAAAG+GGG - chr4.2:22656272-22656291 None:intergenic 35.0%
TCTTATGCCTTTCTTCATCT+TGG - chr4.2:22656137-22656156 None:intergenic 35.0%
TTTCTCCTCAAGAACTCTTT+GGG + chr4.2:22656109-22656128 MS.gene39496:CDS 35.0%
TTTGGAGAGAAAACTAGCAA+TGG + chr4.2:22656771-22656790 MS.gene39496:CDS 35.0%
! AATATAGTGGAATAAGGGGA+AGG + chr4.2:22656383-22656402 MS.gene39496:CDS 35.0%
! CCATGTATAGGTCATCTTTT+GGG + chr4.2:22656494-22656513 MS.gene39496:CDS 35.0%
! TATCCAAGGTTTTCACTTGA+TGG + chr4.2:22656463-22656482 MS.gene39496:CDS 35.0%
! TCCATGTATAGGTCATCTTT+TGG + chr4.2:22656493-22656512 MS.gene39496:CDS 35.0%
!!! TCATCTTTTGGGACTATAGA+AGG + chr4.2:22656505-22656524 MS.gene39496:CDS 35.0%
AAAACTAGCAATGGACATGC+AGG + chr4.2:22656780-22656799 MS.gene39496:CDS 40.0%
AAACTAGCAATGGACATGCA+GGG + chr4.2:22656781-22656800 MS.gene39496:CDS 40.0%
AGTACTATTGGCAATGGCAT+TGG + chr4.2:22656205-22656224 MS.gene39496:CDS 40.0%
ATTAACTTTGCCACAACACC+AGG - chr4.2:22656634-22656653 None:intergenic 40.0%
CCCAAAAGATGACCTATACA+TGG - chr4.2:22656497-22656516 None:intergenic 40.0%
CTTCCATCAAGTGAAAACCT+TGG - chr4.2:22656469-22656488 None:intergenic 40.0%
CTTTCTCCTCAAGAACTCTT+TGG + chr4.2:22656108-22656127 MS.gene39496:CDS 40.0%
GAGTTCTTGAGGAGAAAGAA+TGG - chr4.2:22656106-22656125 None:intergenic 40.0%
TCCAAATCATGCCTCTCAAA+TGG - chr4.2:22656757-22656776 None:intergenic 40.0%
TTGTGATGTCAACTTGGCTA+TGG - chr4.2:22656234-22656253 None:intergenic 40.0%
! CTTTTTCTACCTACTGACAC+TGG - chr4.2:22656673-22656692 None:intergenic 40.0%
! GGTGTTGTGGCAAAGTTAAT+GGG + chr4.2:22656634-22656653 MS.gene39496:CDS 40.0%
!! GGATGAGTAGTGTTGATTGT+AGG + chr4.2:22656710-22656729 MS.gene39496:CDS 40.0%
!! TGGTGTTGTGGCAAAGTTAA+TGG + chr4.2:22656633-22656652 MS.gene39496:CDS 40.0%
!! TGTGGCAAAGTTAATGGGTT+TGG + chr4.2:22656639-22656658 MS.gene39496:CDS 40.0%
AAATGGAGGAAGAGATGGCA+AGG + chr4.2:22656344-22656363 MS.gene39496:CDS 45.0%
AGTTGACATCACAAGCAGCA+AGG + chr4.2:22656240-22656259 MS.gene39496:CDS 45.0%
GAAGCTATTCCAGTGTCAGT+AGG + chr4.2:22656661-22656680 MS.gene39496:CDS 45.0%
TAAGAAGGAGGCCATTTGAG+AGG + chr4.2:22656743-22656762 MS.gene39496:CDS 45.0%
TTGGGAGCCAAGATGAAGAA+AGG + chr4.2:22656127-22656146 MS.gene39496:CDS 45.0%
! ACCGGTTTCTCTAGAAGCTA+TGG + chr4.2:22656876-22656895 MS.gene39496:CDS 45.0%
! GCCATAGCTTCTAGAGAAAC+CGG - chr4.2:22656880-22656899 None:intergenic 45.0%
! GCCATTTGAGAGGCATGATT+TGG + chr4.2:22656753-22656772 MS.gene39496:CDS 45.0%
! GTTTCTCTAGAAGCTATGGC+AGG + chr4.2:22656880-22656899 MS.gene39496:CDS 45.0%
! GTTTTCCACCAACAGTTGCT+GGG + chr4.2:22656587-22656606 MS.gene39496:CDS 45.0%
! TGCTGCTTGTGATGTCAACT+TGG - chr4.2:22656240-22656259 None:intergenic 45.0%
! TGTTTTCCACCAACAGTTGC+TGG + chr4.2:22656586-22656605 MS.gene39496:CDS 45.0%
! TTGGTGTAAACCTGGTGTTG+TGG + chr4.2:22656621-22656640 MS.gene39496:CDS 45.0%
! TTTTCCACCAACAGTTGCTG+GGG + chr4.2:22656588-22656607 MS.gene39496:CDS 45.0%
!! AACGTGGTTTGGTGTAAACC+TGG + chr4.2:22656613-22656632 MS.gene39496:CDS 45.0%
CTCCTTGGTTGCCAACTACT+AGG - chr4.2:22656910-22656929 None:intergenic 50.0%
GTCCTAGTAGTTGGCAACCA+AGG + chr4.2:22656905-22656924 MS.gene39496:CDS 50.0%
TGGCTCCCAAAGAGTTCTTG+AGG - chr4.2:22656117-22656136 None:intergenic 50.0%
! TCTCTAGAAGCTATGGCAGG+AGG + chr4.2:22656883-22656902 MS.gene39496:CDS 50.0%
AACAGTTGCTGGGGAGAACG+TGG + chr4.2:22656597-22656616 MS.gene39496:CDS 55.0%
ACGTTCTCCCCAGCAACTGT+TGG - chr4.2:22656598-22656617 None:intergenic 55.0%
TGGCAGGAGGTCCTAGTAGT+TGG + chr4.2:22656896-22656915 MS.gene39496:CDS 55.0%
TTCTCCCCAGCAACTGTTGG+TGG - chr4.2:22656595-22656614 None:intergenic 55.0%
TTGCTGGGGAGAACGTGGTT+TGG + chr4.2:22656602-22656621 MS.gene39496:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22656100 22656939 22656100 ID=MS.gene39496
chr4.2 mRNA 22656100 22656939 22656100 ID=MS.gene39496.t1;Parent=MS.gene39496
chr4.2 exon 22656100 22656261 22656100 ID=MS.gene39496.t1.exon1;Parent=MS.gene39496.t1
chr4.2 CDS 22656100 22656261 22656100 ID=cds.MS.gene39496.t1;Parent=MS.gene39496.t1
chr4.2 exon 22656304 22656939 22656304 ID=MS.gene39496.t1.exon2;Parent=MS.gene39496.t1
chr4.2 CDS 22656304 22656939 22656304 ID=cds.MS.gene39496.t1;Parent=MS.gene39496.t1
Gene Sequence

>MS.gene39496

ATGCCATTCTTTCTCCTCAAGAACTCTTTGGGAGCCAAGATGAAGAAAGGCATAAGAACTTTCTGCAACACTGATGTGTCAACCTCAACTCTAAACCAACAAAATAGTACTATTGGCAATGGCATTGGCAACCATAGCCAAGTTGACATCACAAGCAGCAAGGTAAGCTCCCCTTTTTTGCAATGTGACAACTCAAATTCAAAGCAAAGCACACCAACATTAGAAGATCTTATACTACAATTAGAAATGGAGGAAGAGATGGCAAGGAAAGAAAAACTCAATGAATATAGTGGAATAAGGGGAAGGATGTCATGTGTTAACAACTCTGACATATTAAGATCTGCAAGGAATGCTTTGAATCAATATCCAAGGTTTTCACTTGATGGAAGAGATTCCATGTATAGGTCATCTTTTGGGACTATAGAAGGAAGAAGATCAGTTTGTAGTGAGATAAGTTTAGAACCAAAGCTTGATGAAAAGGTTATGTGTTTTCCACCAACAGTTGCTGGGGAGAACGTGGTTTGGTGTAAACCTGGTGTTGTGGCAAAGTTAATGGGTTTGGAAGCTATTCCAGTGTCAGTAGGTAGAAAAAGATCTGACAAAAAAGAGAGGATGAGTAGTGTTGATTGTAGGAGACAAAATATAAGAAGGAGGCCATTTGAGAGGCATGATTTGGAGAGAAAACTAGCAATGGACATGCAGGGTTATCGTGATCACATTGTTAGAAGAAGAAATAGGTCTGGTTTTTGCTCTAAAAATGGATATTGTATTATGAAACCGGTTTCTCTAGAAGCTATGGCAGGAGGTCCTAGTAGTTGGCAACCAAGGAGATATGTTTAG

Protein sequence

>MS.gene39496.t1

MPFFLLKNSLGAKMKKGIRTFCNTDVSTSTLNQQNSTIGNGIGNHSQVDITSSKQSTPTLEDLILQLEMEEEMARKEKLNEYSGIRGRMSCVNNSDILRSARNALNQYPRFSLDGRDSMYRSSFGTIEGRRSVCSEISLEPKLDEKVMCFPPTVAGENVVWCKPGVVAKLMGLEAIPVSVGRKRSDKKERMSSVDCRRQNIRRRPFERHDLERKLAMDMQGYRDHIVRRRNRSGFCSKNGYCIMKPVSLEAMAGGPSSWQPRRYV