AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43874


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43874.t1 MTR_2g095130 84.066 182 26 1 8 186 39 220 4.50e-105 301
MS.gene43874.t1 MTR_2g095060 76.243 181 41 1 8 186 39 219 1.84e-92 269
MS.gene43874.t1 MTR_2g095080 76.630 184 38 2 8 186 39 222 7.55e-92 268
MS.gene43874.t1 MTR_2g095170 93.966 116 7 0 5 120 36 151 1.57e-74 222
MS.gene43874.t1 MTR_4g036290 57.639 144 57 3 44 184 44 186 8.16e-51 162
MS.gene43874.t1 MTR_7g077050 58.108 148 57 4 40 184 39 184 1.11e-50 162
MS.gene43874.t1 MTR_7g077110 58.108 148 57 4 40 184 41 186 1.33e-50 162
MS.gene43874.t1 MTR_7g077000 60.156 128 49 2 58 184 2 128 6.26e-49 155
MS.gene43874.t1 MTR_8g031170 60.305 131 50 2 55 184 61 190 1.31e-48 157
MS.gene43874.t1 MTR_7g076990 56.757 148 59 4 40 184 41 186 2.72e-48 156
MS.gene43874.t1 MTR_4g036300 56.757 148 59 4 40 184 41 186 2.72e-48 156
MS.gene43874.t1 MTR_7g012350 56.081 148 60 4 40 184 41 186 7.39e-48 155
MS.gene43874.t1 MTR_7g076770 60.938 128 48 2 58 184 61 187 8.58e-48 155
MS.gene43874.t1 MTR_7g076920 56.081 148 60 4 40 184 41 186 9.09e-48 154
MS.gene43874.t1 MTR_7g077010 57.576 132 54 2 54 184 57 187 4.58e-47 153
MS.gene43874.t1 MTR_7g077090 53.247 154 64 4 34 184 39 187 5.10e-47 153
MS.gene43874.t1 MTR_7g012340 54.730 148 62 4 40 184 41 186 7.40e-47 152
MS.gene43874.t1 MTR_7g076960 55.102 147 62 4 40 184 39 183 1.61e-46 151
MS.gene43874.t1 MTR_4g036410 57.252 131 54 2 55 184 39 168 7.36e-46 149
MS.gene43874.t1 MTR_7g076970 57.252 131 54 2 55 184 58 187 1.09e-45 149
MS.gene43874.t1 MTR_8g031120 51.754 114 43 3 72 184 34 136 1.29e-30 109
MS.gene43874.t1 MTR_8g031150 51.754 114 43 3 72 184 42 144 1.05e-29 107
MS.gene43874.t1 MTR_8g031160 49.107 112 42 2 72 183 24 120 5.57e-27 99.8
MS.gene43874.t1 MTR_7g077040 43.966 116 38 2 55 170 36 124 5.47e-23 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCTGTGTGGAATGCTTT+AGG 0.246178 2.2:+6485907 MS.gene43874:CDS
TAGCTGTGTGGAATGCTTTA+GGG 0.269715 2.2:+6485908 MS.gene43874:CDS
TGTCGTAACAATATTGTTGA+TGG 0.347026 2.2:+6485880 MS.gene43874:CDS
TGATTCTCCGTCGCACTCTA+AGG 0.363482 2.2:-6485566 None:intergenic
CGTTGTACCATCCGGTGGAC+AGG 0.400359 2.2:-6485732 None:intergenic
CATATGTTGGTCCCAACGTC+GGG 0.409197 2.2:-6485490 None:intergenic
CCAGTTACATGGTCTATGTC+TGG 0.410397 2.2:+6485955 MS.gene43874:CDS
GGCGACAAGTGTAAATCTTC+CGG 0.412697 2.2:+6485541 MS.gene43874:CDS
TTAGAATCACCGCGAGGAAC+GGG 0.413213 2.2:+6485779 MS.gene43874:CDS
TTGTCGCCACTTGGTGGTGG+AGG 0.433015 2.2:-6485526 None:intergenic
CCACTTGGTGGTGGAGGGGA+CGG 0.436308 2.2:-6485520 None:intergenic
CCAGACATAGACCATGTAAC+TGG 0.445756 2.2:-6485955 None:intergenic
GCAACGATGACCCCGACGTT+GGG 0.448979 2.2:+6485479 MS.gene43874:CDS
TGGTGGTGGAGGGGACGGTT+TGG 0.450078 2.2:-6485515 None:intergenic
CTACTCTTTCTGAGTTGCTA+TGG 0.454282 2.2:-6485705 None:intergenic
GTGGAGAGCACTTGAATTGA+GGG 0.454649 2.2:-6485591 None:intergenic
AGAGTAGTGGCCCTGTCCAC+CGG 0.488313 2.2:+6485721 MS.gene43874:CDS
CACTTGTCGCCACTTGGTGG+TGG 0.492715 2.2:-6485529 None:intergenic
GATTTCAGCGAGGGTGGCGA+TGG 0.507805 2.2:+6485652 MS.gene43874:CDS
GGTGGAGAGCACTTGAATTG+AGG 0.508261 2.2:-6485592 None:intergenic
TGTCGCCACTTGGTGGTGGA+GGG 0.508955 2.2:-6485525 None:intergenic
ATTAGAATCACCGCGAGGAA+CGG 0.523253 2.2:+6485778 MS.gene43874:CDS
GGACGGTTTGGTGCATATGT+TGG 0.523330 2.2:-6485503 None:intergenic
GTCGCCACTTGGTGGTGGAG+GGG 0.524109 2.2:-6485524 None:intergenic
GACCAGTGTGATTCAGTGAA+TGG 0.526043 2.2:+6485826 MS.gene43874:CDS
GATTTACACTTGTCGCCACT+TGG 0.526065 2.2:-6485535 None:intergenic
TTCAGCGAGGGTGGCGATGG+AGG 0.531365 2.2:+6485655 MS.gene43874:CDS
AATTGCTCGTCGCACTGAGA+TGG 0.531537 2.2:-6485682 None:intergenic
TTACACTTGTCGCCACTTGG+TGG 0.536650 2.2:-6485532 None:intergenic
TGAACCTCCGTTGTACCATC+CGG 0.538055 2.2:-6485740 None:intergenic
GGTCAACTCATTTGCATCAA+CGG 0.540764 2.2:+6485451 MS.gene43874:CDS
TTAGGGTTGGATACTGATGA+TGG 0.540804 2.2:+6485925 MS.gene43874:CDS
TCCGTCGCACTCTAAGGTGC+CGG 0.545674 2.2:-6485560 None:intergenic
GCTTGTGTGGAAGATGAAAC+TGG 0.550048 2.2:-6485613 None:intergenic
GAGGAGTGTGATGGCGAAAG+TGG 0.551844 2.2:+6485801 MS.gene43874:CDS
GCATATGTTGGTCCCAACGT+CGG 0.558869 2.2:-6485491 None:intergenic
GTTGTACCATCCGGTGGACA+GGG 0.562020 2.2:-6485731 None:intergenic
TGCAACGATGACCCCGACGT+TGG 0.562513 2.2:+6485478 MS.gene43874:CDS
TTGAATGATTTCAGCGAGGG+TGG 0.573268 2.2:+6485646 MS.gene43874:CDS
ATGGTGTGGTGCCAGTTACA+TGG 0.574649 2.2:+6485944 MS.gene43874:CDS
GAGGAACGGGAGGAGTGTGA+TGG 0.583244 2.2:+6485792 MS.gene43874:CDS
GAATCACCGCGAGGAACGGG+AGG 0.586047 2.2:+6485782 MS.gene43874:CDS
AAACTCTCAAACGACTGCGC+TGG 0.587411 2.2:+6485430 MS.gene43874:CDS
ACGTTGAATGATTTCAGCGA+GGG 0.598557 2.2:+6485643 MS.gene43874:CDS
CTGTCCACCGGATGGTACAA+CGG 0.625342 2.2:+6485733 MS.gene43874:CDS
GAGTGTGATGGCGAAAGTGG+TGG 0.626341 2.2:+6485804 MS.gene43874:CDS
GTTGGATACTGATGATGGTG+TGG 0.638034 2.2:+6485930 MS.gene43874:CDS
TACAACGGAGGTTCAAGATG+TGG 0.638491 2.2:+6485748 MS.gene43874:CDS
CACACTCCTCCCGTTCCTCG+CGG 0.641101 2.2:-6485788 None:intergenic
TAGTGGCCCTGTCCACCGGA+TGG 0.641578 2.2:+6485725 MS.gene43874:CDS
GGATGTGATGAGGAACATGC+AGG 0.645551 2.2:+6485847 MS.gene43874:CDS
TGTGTGGAATGCTTTAGGGT+TGG 0.646152 2.2:+6485912 MS.gene43874:CDS
CCGTCCCCTCCACCACCAAG+TGG 0.646637 2.2:+6485520 MS.gene43874:CDS
ATCCATTCACTGAATCACAC+TGG 0.661311 2.2:-6485828 None:intergenic
CAACGTGAGTAATGCTTGTG+TGG 0.662007 2.2:-6485626 None:intergenic
ACCTCCGTTGTACCATCCGG+TGG 0.666402 2.2:-6485737 None:intergenic
TCCGGCACCTTAGAGTGCGA+CGG 0.673293 2.2:+6485559 MS.gene43874:CDS
TAGCAACTCAGAAAGAGTAG+TGG 0.673721 2.2:+6485708 MS.gene43874:CDS
CACGTTGAATGATTTCAGCG+AGG 0.674472 2.2:+6485642 MS.gene43874:CDS
TTCAGTGAATGGATGTGATG+AGG 0.675998 2.2:+6485837 MS.gene43874:CDS
ATATGTTGGTCCCAACGTCG+GGG 0.692316 2.2:-6485489 None:intergenic
ACAATATTGTTACGACAAGG+TGG 0.698524 2.2:-6485874 None:intergenic
TCAACAATATTGTTACGACA+AGG 0.725534 2.2:-6485877 None:intergenic
TTGATGGATCTGTAGCTGTG+TGG 0.729799 2.2:+6485896 MS.gene43874:CDS
TCCACCGGATGGTACAACGG+AGG 0.763995 2.2:+6485736 MS.gene43874:CDS
TGTGTGGAAGATGAAACTGG+TGG 0.770461 2.2:-6485610 None:intergenic
AAATGATTAGAATCACCGCG+AGG 0.806861 2.2:+6485773 MS.gene43874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCAACAATATTGTTACGACA+AGG - chr2.2:6485880-6485899 None:intergenic 30.0%
TGTCGTAACAATATTGTTGA+TGG + chr2.2:6485880-6485899 MS.gene43874:CDS 30.0%
ACAATATTGTTACGACAAGG+TGG - chr2.2:6485877-6485896 None:intergenic 35.0%
! AGTCGTTTGAGAGTTTTACA+AGG - chr2.2:6485426-6485445 None:intergenic 35.0%
AAATGATTAGAATCACCGCG+AGG + chr2.2:6485773-6485792 MS.gene43874:CDS 40.0%
ACGTTGAATGATTTCAGCGA+GGG + chr2.2:6485643-6485662 MS.gene43874:CDS 40.0%
ATCCATTCACTGAATCACAC+TGG - chr2.2:6485831-6485850 None:intergenic 40.0%
CTACTCTTTCTGAGTTGCTA+TGG - chr2.2:6485708-6485727 None:intergenic 40.0%
GGTCAACTCATTTGCATCAA+CGG + chr2.2:6485451-6485470 MS.gene43874:CDS 40.0%
TAGCAACTCAGAAAGAGTAG+TGG + chr2.2:6485708-6485727 MS.gene43874:CDS 40.0%
TAGCTGTGTGGAATGCTTTA+GGG + chr2.2:6485908-6485927 MS.gene43874:CDS 40.0%
TTAGGGTTGGATACTGATGA+TGG + chr2.2:6485925-6485944 MS.gene43874:CDS 40.0%
TTCAGTGAATGGATGTGATG+AGG + chr2.2:6485837-6485856 MS.gene43874:CDS 40.0%
ATTAGAATCACCGCGAGGAA+CGG + chr2.2:6485778-6485797 MS.gene43874:CDS 45.0%
CAACGTGAGTAATGCTTGTG+TGG - chr2.2:6485629-6485648 None:intergenic 45.0%
CACGTTGAATGATTTCAGCG+AGG + chr2.2:6485642-6485661 MS.gene43874:CDS 45.0%
CCAGACATAGACCATGTAAC+TGG - chr2.2:6485958-6485977 None:intergenic 45.0%
CCAGTTACATGGTCTATGTC+TGG + chr2.2:6485955-6485974 MS.gene43874:CDS 45.0%
GACCAGTGTGATTCAGTGAA+TGG + chr2.2:6485826-6485845 MS.gene43874:CDS 45.0%
GATTTACACTTGTCGCCACT+TGG - chr2.2:6485538-6485557 None:intergenic 45.0%
GCTTGTGTGGAAGATGAAAC+TGG - chr2.2:6485616-6485635 None:intergenic 45.0%
GGCGACAAGTGTAAATCTTC+CGG + chr2.2:6485541-6485560 MS.gene43874:CDS 45.0%
GTAGCTGTGTGGAATGCTTT+AGG + chr2.2:6485907-6485926 MS.gene43874:CDS 45.0%
TACAACGGAGGTTCAAGATG+TGG + chr2.2:6485748-6485767 MS.gene43874:CDS 45.0%
TGTGTGGAAGATGAAACTGG+TGG - chr2.2:6485613-6485632 None:intergenic 45.0%
TTGAATGATTTCAGCGAGGG+TGG + chr2.2:6485646-6485665 MS.gene43874:CDS 45.0%
! TTGATGGATCTGTAGCTGTG+TGG + chr2.2:6485896-6485915 MS.gene43874:CDS 45.0%
!! GTGGAGAGCACTTGAATTGA+GGG - chr2.2:6485594-6485613 None:intergenic 45.0%
!! GTTGGATACTGATGATGGTG+TGG + chr2.2:6485930-6485949 MS.gene43874:CDS 45.0%
!! TGTGTGGAATGCTTTAGGGT+TGG + chr2.2:6485912-6485931 MS.gene43874:CDS 45.0%
AAACTCTCAAACGACTGCGC+TGG + chr2.2:6485430-6485449 MS.gene43874:CDS 50.0%
AATTGCTCGTCGCACTGAGA+TGG - chr2.2:6485685-6485704 None:intergenic 50.0%
ATATGTTGGTCCCAACGTCG+GGG - chr2.2:6485492-6485511 None:intergenic 50.0%
CATATGTTGGTCCCAACGTC+GGG - chr2.2:6485493-6485512 None:intergenic 50.0%
GCATATGTTGGTCCCAACGT+CGG - chr2.2:6485494-6485513 None:intergenic 50.0%
GGATGTGATGAGGAACATGC+AGG + chr2.2:6485847-6485866 MS.gene43874:CDS 50.0%
TGAACCTCCGTTGTACCATC+CGG - chr2.2:6485743-6485762 None:intergenic 50.0%
TGATTCTCCGTCGCACTCTA+AGG - chr2.2:6485569-6485588 None:intergenic 50.0%
TTACACTTGTCGCCACTTGG+TGG - chr2.2:6485535-6485554 None:intergenic 50.0%
TTAGAATCACCGCGAGGAAC+GGG + chr2.2:6485779-6485798 MS.gene43874:CDS 50.0%
! ATGGTGTGGTGCCAGTTACA+TGG + chr2.2:6485944-6485963 MS.gene43874:CDS 50.0%
!! GGACGGTTTGGTGCATATGT+TGG - chr2.2:6485506-6485525 None:intergenic 50.0%
!! GGTGGAGAGCACTTGAATTG+AGG - chr2.2:6485595-6485614 None:intergenic 50.0%
CTGTCCACCGGATGGTACAA+CGG + chr2.2:6485733-6485752 MS.gene43874:CDS 55.0%
GTTGTACCATCCGGTGGACA+GGG - chr2.2:6485734-6485753 None:intergenic 55.0%
! GAGGAGTGTGATGGCGAAAG+TGG + chr2.2:6485801-6485820 MS.gene43874:CDS 55.0%
! GAGTGTGATGGCGAAAGTGG+TGG + chr2.2:6485804-6485823 MS.gene43874:CDS 55.0%
ACCTCCGTTGTACCATCCGG+TGG - chr2.2:6485740-6485759 None:intergenic 60.0%
AGAGTAGTGGCCCTGTCCAC+CGG + chr2.2:6485721-6485740 MS.gene43874:CDS 60.0%
CGTTGTACCATCCGGTGGAC+AGG - chr2.2:6485735-6485754 None:intergenic 60.0%
GATTTCAGCGAGGGTGGCGA+TGG + chr2.2:6485652-6485671 MS.gene43874:CDS 60.0%
GCAACGATGACCCCGACGTT+GGG + chr2.2:6485479-6485498 MS.gene43874:CDS 60.0%
TCCACCGGATGGTACAACGG+AGG + chr2.2:6485736-6485755 MS.gene43874:CDS 60.0%
TCCGGCACCTTAGAGTGCGA+CGG + chr2.2:6485559-6485578 MS.gene43874:CDS 60.0%
TGCAACGATGACCCCGACGT+TGG + chr2.2:6485478-6485497 MS.gene43874:CDS 60.0%
! CACTTGTCGCCACTTGGTGG+TGG - chr2.2:6485532-6485551 None:intergenic 60.0%
! GAGGAACGGGAGGAGTGTGA+TGG + chr2.2:6485792-6485811 MS.gene43874:CDS 60.0%
!! TCCGTCGCACTCTAAGGTGC+CGG - chr2.2:6485563-6485582 None:intergenic 60.0%
!! TGTCGCCACTTGGTGGTGGA+GGG - chr2.2:6485528-6485547 None:intergenic 60.0%
!! TTGTCGCCACTTGGTGGTGG+AGG - chr2.2:6485529-6485548 None:intergenic 60.0%
CACACTCCTCCCGTTCCTCG+CGG - chr2.2:6485791-6485810 None:intergenic 65.0%
GAATCACCGCGAGGAACGGG+AGG + chr2.2:6485782-6485801 MS.gene43874:CDS 65.0%
TAGTGGCCCTGTCCACCGGA+TGG + chr2.2:6485725-6485744 MS.gene43874:CDS 65.0%
TTCAGCGAGGGTGGCGATGG+AGG + chr2.2:6485655-6485674 MS.gene43874:CDS 65.0%
!! CCACTTGGTGGTGGAGGGGA+CGG - chr2.2:6485523-6485542 None:intergenic 65.0%
!! GTCGCCACTTGGTGGTGGAG+GGG - chr2.2:6485527-6485546 None:intergenic 65.0%
!! TGGTGGTGGAGGGGACGGTT+TGG - chr2.2:6485518-6485537 None:intergenic 65.0%
CCGTCCCCTCCACCACCAAG+TGG + chr2.2:6485520-6485539 MS.gene43874:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 6485418 6486011 6485418 ID=MS.gene43874
chr2.2 mRNA 6485418 6486011 6485418 ID=MS.gene43874.t1;Parent=MS.gene43874
chr2.2 exon 6485418 6486011 6485418 ID=MS.gene43874.t1.exon1;Parent=MS.gene43874.t1
chr2.2 CDS 6485418 6486011 6485418 ID=cds.MS.gene43874.t1;Parent=MS.gene43874.t1
Gene Sequence

>MS.gene43874

ATGGACCTTGTAAAACTCTCAAACGACTGCGCTGGTCAACTCATTTGCATCAACGGAAAATGCAACGATGACCCCGACGTTGGGACCAACATATGCACCAAACCGTCCCCTCCACCACCAAGTGGCGACAAGTGTAAATCTTCCGGCACCTTAGAGTGCGACGGAGAATCATTCCCTCAATTCAAGTGCTCTCCACCAGTTTCATCTTCCACACAAGCATTACTCACGTTGAATGATTTCAGCGAGGGTGGCGATGGAGGAGCTCCATCTCAGTGCGACGAGCAATTCCATAGCAACTCAGAAAGAGTAGTGGCCCTGTCCACCGGATGGTACAACGGAGGTTCAAGATGTGGAAAAATGATTAGAATCACCGCGAGGAACGGGAGGAGTGTGATGGCGAAAGTGGTGGACCAGTGTGATTCAGTGAATGGATGTGATGAGGAACATGCAGGTCAGCCACCTTGTCGTAACAATATTGTTGATGGATCTGTAGCTGTGTGGAATGCTTTAGGGTTGGATACTGATGATGGTGTGGTGCCAGTTACATGGTCTATGTCTGGATCTAATGAAGCTTATGAAACATTTAATGCCTAA

Protein sequence

>MS.gene43874.t1

MDLVKLSNDCAGQLICINGKCNDDPDVGTNICTKPSPPPPSGDKCKSSGTLECDGESFPQFKCSPPVSSSTQALLTLNDFSEGGDGGAPSQCDEQFHSNSERVVALSTGWYNGGSRCGKMIRITARNGRSVMAKVVDQCDSVNGCDEEHAGQPPCRNNIVDGSVAVWNALGLDTDDGVVPVTWSMSGSNEAYETFNA