Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44583.t1 | XP_003619416.1 | 98.9 | 524 | 6 | 0 | 1 | 524 | 1 | 524 | 9.70E-269 | 935.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44583.t1 | Q0WMN5 | 50.3 | 457 | 207 | 6 | 65 | 517 | 59 | 499 | 1.1e-87 | 325.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44583.t1 | G7KLG5 | 98.9 | 524 | 6 | 0 | 1 | 524 | 1 | 524 | 7.0e-269 | 935.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene44583 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene44583 | 0.852355 | 4.87E-61 | -1.69E-46 |
| MS.gene049222 | MS.gene44583 | 0.837516 | 4.96E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene44583 | 0.815078 | 1.11E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene44583 | 0.817019 | 4.10E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene44583 | 0.86529 | 6.58E-65 | -1.69E-46 |
| MS.gene049609 | MS.gene44583 | 0.810137 | 1.34E-50 | -1.69E-46 |
| MS.gene049615 | MS.gene44583 | 0.812008 | 5.26E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene44583 | 0.844757 | 6.20E-59 | -1.69E-46 |
| MS.gene050165 | MS.gene44583 | 0.824482 | 7.88E-54 | -1.69E-46 |
| MS.gene050333 | MS.gene44583 | 0.820637 | 6.17E-53 | -1.69E-46 |
| MS.gene050353 | MS.gene44583 | -0.806866 | 6.68E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene44583 | 0.811352 | 7.31E-51 | -1.69E-46 |
| MS.gene050518 | MS.gene44583 | 0.845524 | 3.85E-59 | -1.69E-46 |
| MS.gene050538 | MS.gene44583 | 0.80396 | 2.72E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene44583 | 0.836318 | 1.00E-56 | -1.69E-46 |
| MS.gene050675 | MS.gene44583 | 0.834689 | 2.59E-56 | -1.69E-46 |
| MS.gene050699 | MS.gene44583 | 0.830973 | 2.17E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene44583 | 0.825003 | 5.94E-54 | -1.69E-46 |
| MS.gene050767 | MS.gene44583 | 0.813661 | 2.29E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene44583 | 0.814673 | 1.37E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene44583 | 0.819042 | 1.43E-52 | -1.69E-46 |
| MS.gene050781 | MS.gene44583 | 0.817514 | 3.17E-52 | -1.69E-46 |
| MS.gene050783 | MS.gene44583 | 0.832672 | 8.27E-56 | -1.69E-46 |
| MS.gene050950 | MS.gene44583 | 0.830291 | 3.19E-55 | -1.69E-46 |
| MS.gene050986 | MS.gene44583 | 0.822134 | 2.79E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene44583 | 0.85688 | 2.38E-62 | -1.69E-46 |
| MS.gene051666 | MS.gene44583 | 0.805002 | 1.65E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene44583 | 0.819201 | 1.32E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene44583 | 0.832011 | 1.21E-55 | -1.69E-46 |
| MS.gene052379 | MS.gene44583 | 0.83522 | 1.90E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene44583 | 0.816763 | 4.68E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene44583 | 0.807578 | 4.72E-50 | -1.69E-46 |
| MS.gene053107 | MS.gene44583 | 0.850942 | 1.23E-60 | -1.69E-46 |
| MS.gene053203 | MS.gene44583 | 0.800618 | 1.32E-48 | -1.69E-46 |
| MS.gene053207 | MS.gene44583 | 0.849067 | 4.10E-60 | -1.69E-46 |
| MS.gene053213 | MS.gene44583 | 0.84609 | 2.70E-59 | -1.69E-46 |
| MS.gene053286 | MS.gene44583 | 0.838667 | 2.51E-57 | -1.69E-46 |
| MS.gene053322 | MS.gene44583 | 0.828696 | 7.79E-55 | -1.69E-46 |
| MS.gene053574 | MS.gene44583 | 0.805237 | 1.47E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene44583 | 0.817842 | 2.67E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene44583 | 0.830293 | 3.19E-55 | -1.69E-46 |
| MS.gene054600 | MS.gene44583 | 0.806856 | 6.71E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene44583 | 0.82906 | 6.36E-55 | -1.69E-46 |
| MS.gene054802 | MS.gene44583 | 0.829824 | 4.15E-55 | -1.69E-46 |
| MS.gene054803 | MS.gene44583 | 0.833405 | 5.43E-56 | -1.69E-46 |
| MS.gene054804 | MS.gene44583 | 0.83338 | 5.51E-56 | -1.69E-46 |
| MS.gene055206 | MS.gene44583 | 0.808755 | 2.65E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene44583 | 0.844729 | 6.31E-59 | -1.69E-46 |
| MS.gene055499 | MS.gene44583 | 0.846151 | 2.60E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene44583 | 0.80934 | 1.99E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene44583 | 0.805131 | 1.55E-49 | -1.69E-46 |
| MS.gene055636 | MS.gene44583 | 0.81837 | 2.03E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene44583 | 0.884311 | 2.14E-71 | -1.69E-46 |
| MS.gene055722 | MS.gene44583 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene44583 | 0.816764 | 4.67E-52 | -1.69E-46 |
| MS.gene056083 | MS.gene44583 | 0.823384 | 1.43E-53 | -1.69E-46 |
| MS.gene056374 | MS.gene44583 | 0.816058 | 6.73E-52 | -1.69E-46 |
| MS.gene056549 | MS.gene44583 | 0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene44583 | 0.822227 | 2.65E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene44583 | 0.853355 | 2.52E-61 | -1.69E-46 |
| MS.gene057129 | MS.gene44583 | 0.829727 | 4.38E-55 | -1.69E-46 |
| MS.gene057146 | MS.gene44583 | 0.84101 | 6.16E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene44583 | 0.834039 | 3.77E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene44583 | 0.85766 | 1.40E-62 | -1.69E-46 |
| MS.gene057831 | MS.gene44583 | 0.831147 | 1.97E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene44583 | 0.846166 | 2.58E-59 | -1.69E-46 |
| MS.gene058323 | MS.gene44583 | 0.808416 | 3.13E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene44583 | 0.815034 | 1.14E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene44583 | 0.81153 | 6.69E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene44583 | 0.820186 | 7.83E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene44583 | 0.807134 | 5.86E-50 | -1.69E-46 |
| MS.gene05883 | MS.gene44583 | 0.856297 | 3.54E-62 | -1.69E-46 |
| MS.gene058993 | MS.gene44583 | 0.817159 | 3.81E-52 | -1.69E-46 |
| MS.gene059029 | MS.gene44583 | 0.830523 | 2.80E-55 | -1.69E-46 |
| MS.gene059181 | MS.gene44583 | 0.810495 | 1.12E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene44583 | 0.832918 | 7.19E-56 | -1.69E-46 |
| MS.gene059536 | MS.gene44583 | 0.828342 | 9.48E-55 | -1.69E-46 |
| MS.gene059676 | MS.gene44583 | 0.829466 | 5.07E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene44583 | 0.816934 | 4.28E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene44583 | 0.835324 | 1.79E-56 | -1.69E-46 |
| MS.gene059756 | MS.gene44583 | 0.817413 | 3.34E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene44583 | 0.86572 | 4.81E-65 | -1.69E-46 |
| MS.gene060804 | MS.gene44583 | 0.81781 | 2.72E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene44583 | 0.806441 | 8.21E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene44583 | 0.804273 | 2.34E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene44583 | 0.817996 | 2.47E-52 | -1.69E-46 |
| MS.gene061134 | MS.gene44583 | 0.805053 | 1.61E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene44583 | 0.834502 | 2.89E-56 | -1.69E-46 |
| MS.gene061171 | MS.gene44583 | 0.807825 | 4.18E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene44583 | 0.871996 | 4.46E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44583.t1 | MTR_6g052360 | 98.855 | 524 | 6 | 0 | 1 | 524 | 1 | 524 | 0.0 | 1077 |
| MS.gene44583.t1 | MTR_6g052360 | 98.690 | 458 | 6 | 0 | 1 | 458 | 1 | 458 | 0.0 | 935 |
| MS.gene44583.t1 | MTR_6g052360 | 99.385 | 325 | 2 | 0 | 200 | 524 | 1 | 325 | 0.0 | 664 |
| MS.gene44583.t1 | MTR_5g090150 | 29.306 | 447 | 241 | 13 | 80 | 522 | 65 | 440 | 8.02e-38 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44583.t1 | AT5G24060 | 45.545 | 505 | 231 | 13 | 32 | 523 | 20 | 493 | 2.12e-133 | 396 |
| MS.gene44583.t1 | AT5G24060 | 46.623 | 459 | 217 | 8 | 71 | 523 | 27 | 463 | 4.23e-133 | 394 |
| MS.gene44583.t1 | AT5G24060 | 47.020 | 453 | 212 | 8 | 77 | 523 | 50 | 480 | 1.76e-132 | 394 |
| MS.gene44583.t1 | AT3G49140 | 41.450 | 538 | 253 | 12 | 3 | 517 | 1 | 499 | 8.34e-117 | 354 |
| MS.gene44583.t1 | AT3G59300 | 30.843 | 415 | 221 | 9 | 104 | 515 | 97 | 448 | 2.40e-51 | 182 |
| MS.gene44583.t1 | AT3G59300 | 30.843 | 415 | 221 | 9 | 104 | 515 | 103 | 454 | 3.07e-51 | 182 |
| MS.gene44583.t1 | AT3G59300 | 29.612 | 412 | 197 | 11 | 104 | 515 | 97 | 415 | 5.16e-44 | 161 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43115 | gene | 25328 | 33198 | 25328 | ID=MS.gene44583 |
| 43115 | mRNA | 25328 | 33198 | 25328 | ID=MS.gene44583.t1;Parent=MS.gene44583 |
| 43115 | exon | 33126 | 33198 | 33126 | ID=MS.gene44583.t1.exon1;Parent=MS.gene44583.t1 |
| 43115 | CDS | 33126 | 33198 | 33126 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 32702 | 32807 | 32702 | ID=MS.gene44583.t1.exon2;Parent=MS.gene44583.t1 |
| 43115 | CDS | 32702 | 32807 | 32702 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 29782 | 30004 | 29782 | ID=MS.gene44583.t1.exon3;Parent=MS.gene44583.t1 |
| 43115 | CDS | 29782 | 30004 | 29782 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 28868 | 28970 | 28868 | ID=MS.gene44583.t1.exon4;Parent=MS.gene44583.t1 |
| 43115 | CDS | 28868 | 28970 | 28868 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 28707 | 28774 | 28707 | ID=MS.gene44583.t1.exon5;Parent=MS.gene44583.t1 |
| 43115 | CDS | 28707 | 28774 | 28707 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 27984 | 28160 | 27984 | ID=MS.gene44583.t1.exon6;Parent=MS.gene44583.t1 |
| 43115 | CDS | 27984 | 28160 | 27984 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 27739 | 27867 | 27739 | ID=MS.gene44583.t1.exon7;Parent=MS.gene44583.t1 |
| 43115 | CDS | 27739 | 27867 | 27739 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 27278 | 27613 | 27278 | ID=MS.gene44583.t1.exon8;Parent=MS.gene44583.t1 |
| 43115 | CDS | 27278 | 27613 | 27278 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 26566 | 26724 | 26566 | ID=MS.gene44583.t1.exon9;Parent=MS.gene44583.t1 |
| 43115 | CDS | 26566 | 26724 | 26566 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 26037 | 26132 | 26037 | ID=MS.gene44583.t1.exon10;Parent=MS.gene44583.t1 |
| 43115 | CDS | 26037 | 26132 | 26037 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| 43115 | exon | 25328 | 25432 | 25328 | ID=MS.gene44583.t1.exon11;Parent=MS.gene44583.t1 |
| 43115 | CDS | 25328 | 25432 | 25328 | ID=cds.MS.gene44583.t1;Parent=MS.gene44583.t1 |
| Gene Sequence |
| Protein sequence |
