AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49281


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49281.t1 MTR_1g069910 94.737 76 4 0 34 109 1125 1200 2.15e-43 150
MS.gene49281.t1 MTR_0003s0460 76.000 75 18 0 34 108 1117 1191 3.20e-32 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49281.t1 AT3G54670 85.526 76 11 0 34 109 1127 1202 2.26e-38 136

Find 35 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAATTTATTACTTTATA+CGG 0.210014 1.1:+52049545 None:intergenic
AAGCGTTAGTTGGGGTTTAC+AGG 0.284648 1.1:-52049031 MS.gene49281:CDS
TTGAATGTTGCAAAGGTTGC+TGG 0.339404 1.1:-52049167 MS.gene49281:CDS
GGGAATAGAAGGGGAAGAAG+AGG 0.361180 1.1:-52049575 MS.gene49281:CDS
TAATGACGGTGACGGGCAAT+TGG 0.366212 1.1:-52049609 MS.gene49281:CDS
GGGCAATTGGGGTGTGGTGA+TGG 0.388931 1.1:-52049596 MS.gene49281:CDS
CAGGATGCTGATGCCGGATC+TGG 0.418635 1.1:-52049104 MS.gene49281:CDS
GGAATAGAAGGGGAAGAAGA+GGG 0.421335 1.1:-52049574 MS.gene49281:CDS
ACAAAGCTGAAGCGTTAGTT+GGG 0.423474 1.1:-52049040 MS.gene49281:CDS
TGAAGGAGCAAGGACTAATC+AGG 0.426310 1.1:-52049123 MS.gene49281:CDS
AATGACGGTGACGGGCAATT+GGG 0.442569 1.1:-52049608 MS.gene49281:CDS
GACAAAGCTGAAGCGTTAGT+TGG 0.453493 1.1:-52049041 MS.gene49281:CDS
ATAAACATGGTCACTGAGAT+TGG 0.460733 1.1:-52049643 None:intergenic
GGCAATTGGGGTGTGGTGAT+GGG 0.462881 1.1:-52049595 MS.gene49281:CDS
GGTGTGGTGATGGGAATAGA+AGG 0.482363 1.1:-52049586 MS.gene49281:CDS
TGTGGTGATGGGAATAGAAG+GGG 0.501049 1.1:-52049584 MS.gene49281:CDS
GGTGACGGGCAATTGGGGTG+TGG 0.517394 1.1:-52049602 MS.gene49281:CDS
ACTAATCAGGATGCTGATGC+CGG 0.528971 1.1:-52049110 MS.gene49281:CDS
GGATGAAGTTGATGCTGCAT+TGG 0.534054 1.1:-52049195 MS.gene49281:CDS
GAATAGAAGGGGAAGAAGAG+GGG 0.538833 1.1:-52049573 MS.gene49281:CDS
AATACTCTGAAAACCAGATC+CGG 0.539223 1.1:+52049091 None:intergenic
CAAAATCTTGTGAAGGAGCA+AGG 0.545040 1.1:-52049133 MS.gene49281:CDS
ATTCGTTCAAAATCTTGTGA+AGG 0.547590 1.1:-52049140 MS.gene49281:CDS
GTTTACAGGGACTCTGAAAG+AGG 0.559697 1.1:-52049017 MS.gene49281:CDS
AGCGTTAGTTGGGGTTTACA+GGG 0.560955 1.1:-52049030 MS.gene49281:CDS
GGTTGTGTAATGACGGTGAC+GGG 0.561095 1.1:-52049616 MS.gene49281:CDS
TGGTGGTGGTTGTGTAATGA+CGG 0.578928 1.1:-52049623 MS.gene49281:CDS
GTGTGGTGATGGGAATAGAA+GGG 0.581151 1.1:-52049585 MS.gene49281:CDS
ATGACGGTGACGGGCAATTG+GGG 0.590556 1.1:-52049607 MS.gene49281:CDS
GGACAACTTGAATGTTGCAA+AGG 0.602237 1.1:-52049174 MS.gene49281:CDS
CAAAGCTGAAGCGTTAGTTG+GGG 0.618753 1.1:-52049039 MS.gene49281:CDS
TTTACAGGGACTCTGAAAGA+GGG 0.636295 1.1:-52049016 None:intergenic
ATGGTCACTGAGATTGGTGG+TGG 0.660935 1.1:-52049637 MS.gene49281:CDS
TGGTTGTGTAATGACGGTGA+CGG 0.698634 1.1:-52049617 MS.gene49281:CDS
AACATGGTCACTGAGATTGG+TGG 0.714202 1.1:-52049640 MS.gene49281:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATTTATTACTTTATA+CGG + chr1.1:52049112-52049131 None:intergenic 10.0%
!!! TTGAACAATAATATTTTAGA+TGG - chr1.1:52049286-52049305 MS.gene49281:intron 15.0%
!!! TAGATGGTTGTTTAAATTTT+GGG - chr1.1:52049302-52049321 MS.gene49281:intron 20.0%
!!! TTAGATGGTTGTTTAAATTT+TGG - chr1.1:52049301-52049320 MS.gene49281:intron 20.0%
!!! TTGCTTATATTTTCAGTTTT+AGG - chr1.1:52049416-52049435 MS.gene49281:intron 20.0%
! AAAATAGTTTGATGGAGATT+TGG - chr1.1:52049365-52049384 MS.gene49281:intron 25.0%
! AAACTGAAAATATAAGCAAG+TGG + chr1.1:52049415-52049434 None:intergenic 25.0%
! AGAAAACAAGGTAAAAACAA+TGG + chr1.1:52049225-52049244 None:intergenic 25.0%
! TTTGCCTAAAAATAGTTTGA+TGG - chr1.1:52049357-52049376 MS.gene49281:intron 25.0%
!! ATCTTTGAAAACAGAAAACA+AGG + chr1.1:52049237-52049256 None:intergenic 25.0%
!!! ATCTCCATCAAACTATTTTT+AGG + chr1.1:52049364-52049383 None:intergenic 25.0%
AACAAGGTAAAAACAATGGA+TGG + chr1.1:52049221-52049240 None:intergenic 30.0%
ACTTCATCCAGTTTGAAAAA+TGG + chr1.1:52049448-52049467 None:intergenic 30.0%
ATTCGTTCAAAATCTTGTGA+AGG - chr1.1:52049514-52049533 MS.gene49281:intron 30.0%
!!! ACAATGGATGGTTTTATGAT+TGG + chr1.1:52049209-52049228 None:intergenic 30.0%
!!! CAATGGATGGTTTTATGATT+GGG + chr1.1:52049208-52049227 None:intergenic 30.0%
AAAAACCAGTTGGCACATTA+AGG + chr1.1:52049137-52049156 None:intergenic 35.0%
TCCAGTTTGAAAAATGGTGA+AGG + chr1.1:52049442-52049461 None:intergenic 35.0%
! AAACCATGTTAATGGACATG+TGG + chr1.1:52049392-52049411 None:intergenic 35.0%
! AATACTCTGAAAACCAGATC+CGG + chr1.1:52049566-52049585 None:intergenic 35.0%
AATATGCACCCGTTAGAGTA+AGG + chr1.1:52049178-52049197 None:intergenic 40.0%
ACCCATGAACCTTACTCTAA+CGG - chr1.1:52049166-52049185 MS.gene49281:CDS 40.0%
CAAAATCTTGTGAAGGAGCA+AGG - chr1.1:52049521-52049540 MS.gene49281:intron 40.0%
GCAAGTGGAAACCATGTTAA+TGG + chr1.1:52049400-52049419 None:intergenic 40.0%
GGACAACTTGAATGTTGCAA+AGG - chr1.1:52049480-52049499 MS.gene49281:intron 40.0%
TGGACCATTGAAAAACCAGT+TGG + chr1.1:52049147-52049166 None:intergenic 40.0%
TTGAATGTTGCAAAGGTTGC+TGG - chr1.1:52049487-52049506 MS.gene49281:intron 40.0%
! ACAAAGCTGAAGCGTTAGTT+GGG - chr1.1:52049614-52049633 MS.gene49281:CDS 40.0%
! ACTCTAACGGGTGCATATTA+GGG - chr1.1:52049179-52049198 MS.gene49281:CDS 40.0%
! CTTTTCCTTAATGTGCCAAC+TGG - chr1.1:52049129-52049148 MS.gene49281:CDS 40.0%
! TACTCTAACGGGTGCATATT+AGG - chr1.1:52049178-52049197 MS.gene49281:CDS 40.0%
!! GCCTTCACCATTTTTCAAAC+TGG - chr1.1:52049438-52049457 MS.gene49281:intron 40.0%
!!! TGTGCCAACTGGTTTTTCAA+TGG - chr1.1:52049140-52049159 MS.gene49281:CDS 40.0%
AAGCGTTAGTTGGGGTTTAC+AGG - chr1.1:52049623-52049642 MS.gene49281:CDS 45.0%
ACCCGTTAGAGTAAGGTTCA+TGG + chr1.1:52049171-52049190 None:intergenic 45.0%
ACTAATCAGGATGCTGATGC+CGG - chr1.1:52049544-52049563 MS.gene49281:intron 45.0%
AGCGTTAGTTGGGGTTTACA+GGG - chr1.1:52049624-52049643 MS.gene49281:CDS 45.0%
CCCATGAACCTTACTCTAAC+GGG - chr1.1:52049167-52049186 MS.gene49281:CDS 45.0%
CCCGTTAGAGTAAGGTTCAT+GGG + chr1.1:52049170-52049189 None:intergenic 45.0%
GAATAGAAGGGGAAGAAGAG+GGG - chr1.1:52049081-52049100 MS.gene49281:CDS 45.0%
GGAATAGAAGGGGAAGAAGA+GGG - chr1.1:52049080-52049099 MS.gene49281:CDS 45.0%
GGTCCACATGTCCATTAACA+TGG - chr1.1:52049386-52049405 MS.gene49281:intron 45.0%
GTTAGAGTAAGGTTCATGGG+TGG + chr1.1:52049167-52049186 None:intergenic 45.0%
TGAAGGAGCAAGGACTAATC+AGG - chr1.1:52049531-52049550 MS.gene49281:intron 45.0%
TGGTTGTGTAATGACGGTGA+CGG - chr1.1:52049037-52049056 MS.gene49281:CDS 45.0%
! CAAAGCTGAAGCGTTAGTTG+GGG - chr1.1:52049615-52049634 MS.gene49281:CDS 45.0%
! CTCTAACGGGTGCATATTAG+GGG - chr1.1:52049180-52049199 MS.gene49281:CDS 45.0%
! GACAAAGCTGAAGCGTTAGT+TGG - chr1.1:52049613-52049632 MS.gene49281:CDS 45.0%
! GTTTACAGGGACTCTGAAAG+AGG - chr1.1:52049637-52049656 MS.gene49281:CDS 45.0%
!! GGATGAAGTTGATGCTGCAT+TGG - chr1.1:52049459-52049478 MS.gene49281:intron 45.0%
!! GTGTGGTGATGGGAATAGAA+GGG - chr1.1:52049069-52049088 MS.gene49281:CDS 45.0%
!! TGGTGGTGGTTGTGTAATGA+CGG - chr1.1:52049031-52049050 MS.gene49281:CDS 45.0%
!! TGTGGTGATGGGAATAGAAG+GGG - chr1.1:52049070-52049089 MS.gene49281:CDS 45.0%
ATGGTCACTGAGATTGGTGG+TGG - chr1.1:52049017-52049036 MS.gene49281:CDS 50.0%
GGGAATAGAAGGGGAAGAAG+AGG - chr1.1:52049079-52049098 MS.gene49281:CDS 50.0%
GGTTGTGTAATGACGGTGAC+GGG - chr1.1:52049038-52049057 MS.gene49281:CDS 50.0%
!! AATGACGGTGACGGGCAATT+GGG - chr1.1:52049046-52049065 MS.gene49281:CDS 50.0%
!! GGTGTGGTGATGGGAATAGA+AGG - chr1.1:52049068-52049087 MS.gene49281:CDS 50.0%
!! TAATGACGGTGACGGGCAAT+TGG - chr1.1:52049045-52049064 MS.gene49281:CDS 50.0%
GGCAATTGGGGTGTGGTGAT+GGG - chr1.1:52049059-52049078 MS.gene49281:CDS 55.0%
!! ATGACGGTGACGGGCAATTG+GGG - chr1.1:52049047-52049066 MS.gene49281:CDS 55.0%
CAGGATGCTGATGCCGGATC+TGG - chr1.1:52049550-52049569 MS.gene49281:intron 60.0%
GGGCAATTGGGGTGTGGTGA+TGG - chr1.1:52049058-52049077 MS.gene49281:CDS 60.0%
!! GGTGACGGGCAATTGGGGTG+TGG - chr1.1:52049052-52049071 MS.gene49281:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 52049017 52049659 52049017 ID=MS.gene49281
chr1.1 mRNA 52049017 52049659 52049017 ID=MS.gene49281.t1;Parent=MS.gene49281
chr1.1 exon 52049559 52049659 52049559 ID=MS.gene49281.t1.exon1;Parent=MS.gene49281.t1
chr1.1 CDS 52049559 52049659 52049559 ID=cds.MS.gene49281.t1;Parent=MS.gene49281.t1
chr1.1 exon 52049017 52049244 52049017 ID=MS.gene49281.t1.exon2;Parent=MS.gene49281.t1
chr1.1 CDS 52049017 52049244 52049017 ID=cds.MS.gene49281.t1;Parent=MS.gene49281.t1
Gene Sequence

>MS.gene49281

ATGGTCACTGAGATTGGTGGTGGTTGTGTAATGACGGTGACGGGCAATTGGGGTGTGGTGATGGGAATAGAAGGGGAAGAAGAGGGGTACATCCGTATAAAGTAATAAATTTCTTTTCCTTAATGTGCCAACTGGTTTTTCAATGGTCCACCCATGAACCTTACTCTAACGGGTGCATATTAGGGGCTCCCAATCATAAAACCATCCATTGTTTTTACCTTGTTTTCTGTTTTCAAAGATTTGTATATGCAAACATGAAGACAATATGTTTGAACAATAATATTTTAGATGGTTGTTTAAATTTTGGGAAGAGTTCATTCAAGTAATAGACTGACACATCTTTGCCTAAAAATAGTTTGATGGAGATTTGGTCCACATGTCCATTAACATGGTTTCCACTTGCTTATATTTTCAGTTTTAGGCCTTCACCATTTTTCAAACTGGATGAAGTTGATGCTGCATTGGACAACTTGAATGTTGCAAAGGTTGCTGGCTTTATTCGTTCAAAATCTTGTGAAGGAGCAAGGACTAATCAGGATGCTGATGCCGGATCTGGTTTTCAGAGTATTGTAATATCTCTTAAAGACAGCTTCTATGACAAAGCTGAAGCGTTAGTTGGGGTTTACAGGGACTCTGAAAGAGG

Protein sequence

>MS.gene49281.t1

MVTEIGGGCVMTVTGNWGVVMGIEGEEEGYIRINFRPSPFFKLDEVDAALDNLNVAKVAGFIRSKSCEGARTNQDADAGSGFQSIVISLKDSFYDKAEALVGVYRDSER