Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51836.t1 | XP_024634430.1 | 96.9 | 161 | 4 | 1 | 1 | 161 | 178 | 337 | 3.70E-86 | 327.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51836.t1 | A0A1S3E695 | 84.5 | 161 | 24 | 1 | 1 | 161 | 253 | 412 | 1.4e-74 | 288.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene51836 | 0.851058 | 1.14E-60 | -1.69E-46 |
| MS.gene049343 | MS.gene51836 | 0.800019 | 1.75E-48 | -1.69E-46 |
| MS.gene049404 | MS.gene51836 | 0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene51836 | 0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene049669 | MS.gene51836 | 0.819285 | 1.26E-52 | -1.69E-46 |
| MS.gene049873 | MS.gene51836 | 0.822963 | 1.79E-53 | -1.69E-46 |
| MS.gene050026 | MS.gene51836 | 0.813898 | 2.03E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene51836 | 0.836932 | 7.00E-57 | -1.69E-46 |
| MS.gene050201 | MS.gene51836 | 0.830242 | 3.28E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene51836 | 0.80522 | 1.48E-49 | -1.69E-46 |
| MS.gene050355 | MS.gene51836 | 0.836904 | 7.12E-57 | -1.69E-46 |
| MS.gene050442 | MS.gene51836 | 0.819744 | 9.89E-53 | -1.69E-46 |
| MS.gene050559 | MS.gene51836 | 0.832302 | 1.02E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene51836 | 0.830721 | 2.50E-55 | -1.69E-46 |
| MS.gene050562 | MS.gene51836 | 0.813215 | 2.86E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene51836 | 0.832729 | 8.01E-56 | -1.69E-46 |
| MS.gene050799 | MS.gene51836 | 0.835958 | 1.24E-56 | -1.69E-46 |
| MS.gene050953 | MS.gene51836 | 0.842803 | 2.07E-58 | -1.69E-46 |
| MS.gene051247 | MS.gene51836 | 0.827648 | 1.39E-54 | -1.69E-46 |
| MS.gene051594 | MS.gene51836 | 0.807293 | 5.43E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene51836 | 0.807035 | 6.15E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene51836 | 0.815223 | 1.03E-51 | -1.69E-46 |
| MS.gene051957 | MS.gene51836 | 0.802826 | 4.67E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene51836 | 0.815942 | 7.14E-52 | -1.69E-46 |
| MS.gene052208 | MS.gene51836 | 0.815191 | 1.05E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene51836 | 0.809757 | 1.62E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene51836 | -0.808997 | 2.35E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene51836 | 0.849653 | 2.82E-60 | -1.69E-46 |
| MS.gene052582 | MS.gene51836 | 0.843007 | 1.83E-58 | -1.69E-46 |
| MS.gene052584 | MS.gene51836 | 0.836901 | 7.13E-57 | -1.69E-46 |
| MS.gene052773 | MS.gene51836 | 0.818305 | 2.10E-52 | -1.69E-46 |
| MS.gene052829 | MS.gene51836 | 0.807355 | 5.26E-50 | -1.69E-46 |
| MS.gene052843 | MS.gene51836 | 0.81181 | 5.81E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene51836 | 0.801495 | 8.77E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene51836 | 0.81307 | 3.08E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene51836 | 0.80931 | 2.02E-50 | -1.69E-46 |
| MS.gene053145 | MS.gene51836 | 0.816698 | 4.84E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene51836 | 0.802357 | 5.83E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene51836 | 0.839154 | 1.88E-57 | -1.69E-46 |
| MS.gene053900 | MS.gene51836 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene053953 | MS.gene51836 | 0.80494 | 1.70E-49 | -1.69E-46 |
| MS.gene053966 | MS.gene51836 | 0.805825 | 1.11E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene51836 | 0.844378 | 7.85E-59 | -1.69E-46 |
| MS.gene054236 | MS.gene51836 | 0.818934 | 1.51E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene51836 | -0.817664 | 2.93E-52 | -1.69E-46 |
| MS.gene054405 | MS.gene51836 | 0.836615 | 8.44E-57 | -1.69E-46 |
| MS.gene054472 | MS.gene51836 | 0.803558 | 3.29E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene51836 | 0.80708 | 6.02E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene51836 | 0.823344 | 1.46E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene51836 | 0.815843 | 7.51E-52 | -1.69E-46 |
| MS.gene055586 | MS.gene51836 | 0.800862 | 1.18E-48 | -1.69E-46 |
| MS.gene055589 | MS.gene51836 | 0.800224 | 1.59E-48 | -1.69E-46 |
| MS.gene055778 | MS.gene51836 | 0.804145 | 2.49E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene51836 | 0.807076 | 6.03E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene51836 | 0.857584 | 1.48E-62 | -1.69E-46 |
| MS.gene05604 | MS.gene51836 | 0.82799 | 1.15E-54 | -1.69E-46 |
| MS.gene056050 | MS.gene51836 | 0.811799 | 5.84E-51 | -1.69E-46 |
| MS.gene056252 | MS.gene51836 | 0.800876 | 1.17E-48 | -1.69E-46 |
| MS.gene056366 | MS.gene51836 | 0.840054 | 1.10E-57 | -1.69E-46 |
| MS.gene056498 | MS.gene51836 | 0.8333 | 5.77E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene51836 | 0.8148 | 1.28E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene51836 | 0.839118 | 1.92E-57 | -1.69E-46 |
| MS.gene056776 | MS.gene51836 | 0.806108 | 9.65E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene51836 | 0.847375 | 1.20E-59 | -1.69E-46 |
| MS.gene057027 | MS.gene51836 | 0.805497 | 1.30E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene51836 | 0.838393 | 2.95E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene51836 | 0.822548 | 2.23E-53 | -1.69E-46 |
| MS.gene057314 | MS.gene51836 | 0.809988 | 1.44E-50 | -1.69E-46 |
| MS.gene057414 | MS.gene51836 | 0.811734 | 6.04E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene51836 | 0.852073 | 5.86E-61 | -1.69E-46 |
| MS.gene057693 | MS.gene51836 | 0.809626 | 1.72E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene51836 | 0.802449 | 5.58E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene51836 | 0.816874 | 4.42E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene51836 | 0.833341 | 5.64E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene51836 | 0.83936 | 1.66E-57 | -1.69E-46 |
| MS.gene058829 | MS.gene51836 | 0.82315 | 1.62E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene51836 | 0.84969 | 2.75E-60 | -1.69E-46 |
| MS.gene059272 | MS.gene51836 | 0.82801 | 1.14E-54 | -1.69E-46 |
| MS.gene05954 | MS.gene51836 | 0.838589 | 2.63E-57 | -1.69E-46 |
| MS.gene059584 | MS.gene51836 | 0.814149 | 1.78E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene51836 | 0.816262 | 6.06E-52 | -1.69E-46 |
| MS.gene05979 | MS.gene51836 | 0.809 | 2.35E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene51836 | 0.841022 | 6.11E-58 | -1.69E-46 |
| MS.gene060429 | MS.gene51836 | 0.82702 | 1.97E-54 | -1.69E-46 |
| MS.gene060521 | MS.gene51836 | 0.838933 | 2.14E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene51836 | 0.863525 | 2.34E-64 | -1.69E-46 |
| MS.gene060634 | MS.gene51836 | -0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene060781 | MS.gene51836 | 0.825933 | 3.58E-54 | -1.69E-46 |
| MS.gene060879 | MS.gene51836 | 0.812044 | 5.17E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene51836 | -0.826614 | 2.46E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTTTATCTTGTTGATTCT+GGG | - | 42748:6533-6552 | MS.gene51836:CDS | 25.0% |
| !!! | GAAGGCATTAACTTTAAAAT+AGG | + | 42748:6730-6749 | MS.gene51836:intergenic | 25.0% |
| !!! | TCATTTTATCTTGTTGATTC+TGG | - | 42748:6532-6551 | MS.gene51836:CDS | 25.0% |
| AATGGATGATATAGAACTTG+AGG | - | 42748:6855-6874 | MS.gene51836:CDS | 30.0% | |
| ATAGGAATCATCTTAGAGAT+AGG | - | 42748:6971-6990 | MS.gene51836:CDS | 30.0% | |
| ATATCTTTCACCTCTATAAG+GGG | + | 42748:6584-6603 | MS.gene51836:intergenic | 30.0% | |
| CGAATGTTGAAAACTTAACA+AGG | - | 42748:6914-6933 | MS.gene51836:CDS | 30.0% | |
| CTGTAGTTAAATAACTCTTC+CGG | + | 42748:6649-6668 | MS.gene51836:intergenic | 30.0% | |
| GAAAACTTAACAAGGTTATC+TGG | - | 42748:6922-6941 | MS.gene51836:CDS | 30.0% | |
| GATATCTTTCACCTCTATAA+GGG | + | 42748:6585-6604 | MS.gene51836:intergenic | 30.0% | |
| TGATATCTTTCACCTCTATA+AGG | + | 42748:6586-6605 | MS.gene51836:intergenic | 30.0% | |
| ! | TGATGAGTTGTTTAGGATAT+GGG | - | 42748:6828-6847 | MS.gene51836:CDS | 30.0% |
| ATCATCTTAGAGATAGGATG+TGG | - | 42748:6977-6996 | MS.gene51836:CDS | 35.0% | |
| ATTTGCCTGATGAGTTGTTT+AGG | - | 42748:6821-6840 | MS.gene51836:CDS | 35.0% | |
| CATATCCTAAACAACTCATC+AGG | + | 42748:6829-6848 | MS.gene51836:intergenic | 35.0% | |
| CGGAAGAGTTATTTAACTAC+AGG | - | 42748:6647-6666 | MS.gene51836:CDS | 35.0% | |
| TATCATGCACAAGAATATCG+AGG | - | 42748:6601-6620 | MS.gene51836:CDS | 35.0% | |
| TATCTTTCACCTCTATAAGG+GGG | + | 42748:6583-6602 | MS.gene51836:intergenic | 35.0% | |
| TCATCTTAGAGATAGGATGT+GGG | - | 42748:6978-6997 | MS.gene51836:CDS | 35.0% | |
| TTAACAAGGTTATCTGGTGA+AGG | - | 42748:6928-6947 | MS.gene51836:CDS | 35.0% | |
| TTCACAATTACATACGCAAG+TGG | - | 42748:6797-6816 | MS.gene51836:CDS | 35.0% | |
| ! | AATAACTCTTCCGGACTTTT+TGG | + | 42748:6640-6659 | MS.gene51836:intergenic | 35.0% |
| ! | CTGATGAGTTGTTTAGGATA+TGG | - | 42748:6827-6846 | MS.gene51836:CDS | 35.0% |
| ! | GTTTAGGATATGGGAAGAAA+TGG | - | 42748:6837-6856 | MS.gene51836:CDS | 35.0% |
| !! | ATGACAATTGAGCGTTGTTT+TGG | - | 42748:6685-6704 | MS.gene51836:CDS | 35.0% |
| !! | TGACAATTGAGCGTTGTTTT+GGG | - | 42748:6686-6705 | MS.gene51836:CDS | 35.0% |
| !!! | TGTTTTGGGGTGTTGAAAAA+CGG | - | 42748:6700-6719 | MS.gene51836:CDS | 35.0% |
| ACTATGAGTCTTTGCCTTGA+AGG | + | 42748:6760-6779 | MS.gene51836:intergenic | 40.0% | |
| GGATGATATAGAACTTGAGG+CGG | - | 42748:6858-6877 | MS.gene51836:CDS | 40.0% | |
| TCTTTGCCTTGAAGGTTTGT+AGG | + | 42748:6752-6771 | MS.gene51836:intergenic | 40.0% | |
| !! | GACAATTGAGCGTTGTTTTG+GGG | - | 42748:6687-6706 | MS.gene51836:CDS | 40.0% |
| !!! | GTTTTGGGGTGTTGAAAAAC+GGG | - | 42748:6701-6720 | MS.gene51836:CDS | 40.0% |
| AACGAGGTTCCTAACCTTGA+AGG | - | 42748:6883-6902 | MS.gene51836:CDS | 45.0% | |
| ACGAGGTTCCTAACCTTGAA+GGG | - | 42748:6884-6903 | MS.gene51836:CDS | 45.0% | |
| ACTACAGGCACTCGTCTTTA+AGG | - | 42748:6662-6681 | MS.gene51836:CDS | 45.0% | |
| AGGTAGACAGCCAAAAAGTC+CGG | - | 42748:6627-6646 | MS.gene51836:CDS | 45.0% | |
| ATAAGGGGGAAGAAAACCTC+CGG | + | 42748:6569-6588 | MS.gene51836:intergenic | 45.0% | |
| GATTCTGGGTATCCATGTAC+CGG | - | 42748:6547-6566 | MS.gene51836:CDS | 45.0% | |
| GCACAAGAATATCGAGGTCA+AGG | - | 42748:6607-6626 | MS.gene51836:CDS | 45.0% | |
| TGCCTTCCTACAAACCTTCA+AGG | - | 42748:6743-6762 | MS.gene51836:CDS | 45.0% | |
| TGCCTTGAAGGTTTGTAGGA+AGG | + | 42748:6748-6767 | MS.gene51836:intergenic | 45.0% | |
| ! | GGTTTTCTTCCCCCTTATAG+AGG | - | 42748:6571-6590 | MS.gene51836:CDS | 45.0% |
| CAGCTGAGATGGCAATGGAT+AGG | - | 42748:6953-6972 | MS.gene51836:CDS | 50.0% | |
| CATTCGAGCTCATCCCTTCA+AGG | + | 42748:6900-6919 | MS.gene51836:intergenic | 50.0% | |
| GAGCTCATCCCTTCAAGGTT+AGG | + | 42748:6895-6914 | MS.gene51836:intergenic | 50.0% | |
| GGAAGAAAACCTCCGGTACA+TGG | + | 42748:6562-6581 | MS.gene51836:intergenic | 50.0% | |
| AGAACTTGAGGCGGTGAACG+AGG | - | 42748:6867-6886 | MS.gene51836:CDS | 55.0% | |
| AGGTGCAGCTGAGATGGCAA+TGG | - | 42748:6948-6967 | MS.gene51836:CDS | 55.0% | |
| TCTGGGTATCCATGTACCGG+AGG | - | 42748:6550-6569 | MS.gene51836:CDS | 55.0% | |
| ! | TGGTGAAGGTGCAGCTGAGA+TGG | - | 42748:6942-6961 | MS.gene51836:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42748 | gene | 6530 | 7017 | 6530 | ID=MS.gene51836 |
| 42748 | mRNA | 6530 | 7017 | 6530 | ID=MS.gene51836.t1;Parent=MS.gene51836 |
| 42748 | exon | 6530 | 7017 | 6530 | ID=MS.gene51836.t1.exon1;Parent=MS.gene51836.t1 |
| 42748 | CDS | 6530 | 7017 | 6530 | ID=cds.MS.gene51836.t1;Parent=MS.gene51836.t1 |
| Gene Sequence |
| Protein sequence |
