Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72008.t1 | XP_013457586.1 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 1.40E-30 | 141.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72008.t1 | Q13395 | 47.2 | 72 | 35 | 1 | 8 | 79 | 1443 | 1511 | 2.5e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72008.t1 | A0A072UPJ3 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 1.0e-30 | 141.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene72008 | 0.801522 | 8.65E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene72008 | 0.8119 | 5.56E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene72008 | 0.844704 | 6.41E-59 | -1.69E-46 |
| MS.gene049271 | MS.gene72008 | 0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene72008 | 0.810386 | 1.18E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene72008 | 0.820612 | 6.25E-53 | -1.69E-46 |
| MS.gene049501 | MS.gene72008 | 0.81483 | 1.26E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene72008 | 0.814024 | 1.90E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene72008 | 0.826959 | 2.04E-54 | -1.69E-46 |
| MS.gene049824 | MS.gene72008 | 0.837851 | 4.07E-57 | -1.69E-46 |
| MS.gene049825 | MS.gene72008 | 0.832424 | 9.53E-56 | -1.69E-46 |
| MS.gene049850 | MS.gene72008 | 0.854518 | 1.17E-61 | -1.69E-46 |
| MS.gene049873 | MS.gene72008 | 0.84537 | 4.24E-59 | -1.69E-46 |
| MS.gene050020 | MS.gene72008 | 0.801093 | 1.06E-48 | -1.69E-46 |
| MS.gene050026 | MS.gene72008 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene050273 | MS.gene72008 | 0.832558 | 8.83E-56 | -1.69E-46 |
| MS.gene050355 | MS.gene72008 | 0.807735 | 4.37E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene72008 | 0.821079 | 4.89E-53 | -1.69E-46 |
| MS.gene050561 | MS.gene72008 | 0.815828 | 7.57E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene72008 | 0.807374 | 5.21E-50 | -1.69E-46 |
| MS.gene050759 | MS.gene72008 | 0.811557 | 6.60E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene72008 | 0.808786 | 2.61E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene72008 | 0.801548 | 8.55E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene72008 | 0.801584 | 8.40E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene72008 | 0.804324 | 2.28E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene72008 | 0.857662 | 1.40E-62 | -1.69E-46 |
| MS.gene051279 | MS.gene72008 | 0.843215 | 1.61E-58 | -1.69E-46 |
| MS.gene051388 | MS.gene72008 | 0.800132 | 1.66E-48 | -1.69E-46 |
| MS.gene051574 | MS.gene72008 | 0.812895 | 3.37E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene72008 | 0.842007 | 3.36E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene72008 | 0.822805 | 1.95E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene72008 | 0.847922 | 8.51E-60 | -1.69E-46 |
| MS.gene051720 | MS.gene72008 | 0.878512 | 2.65E-69 | -1.69E-46 |
| MS.gene051744 | MS.gene72008 | 0.855127 | 7.77E-62 | -1.69E-46 |
| MS.gene051957 | MS.gene72008 | 0.83668 | 8.12E-57 | -1.69E-46 |
| MS.gene052516 | MS.gene72008 | 0.806717 | 7.18E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene72008 | 0.87094 | 9.98E-67 | -1.69E-46 |
| MS.gene052582 | MS.gene72008 | 0.859825 | 3.16E-63 | -1.69E-46 |
| MS.gene052584 | MS.gene72008 | 0.871106 | 8.80E-67 | -1.69E-46 |
| MS.gene052773 | MS.gene72008 | 0.825965 | 3.52E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene72008 | 0.811384 | 7.19E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene72008 | 0.81102 | 8.63E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene72008 | 0.804669 | 1.93E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene72008 | 0.804748 | 1.86E-49 | -1.69E-46 |
| MS.gene053122 | MS.gene72008 | 0.810255 | 1.26E-50 | -1.69E-46 |
| MS.gene053221 | MS.gene72008 | 0.805608 | 1.23E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene72008 | 0.868198 | 7.80E-66 | -1.69E-46 |
| MS.gene053296 | MS.gene72008 | 0.814606 | 1.41E-51 | -1.69E-46 |
| MS.gene053599 | MS.gene72008 | 0.826159 | 3.16E-54 | -1.69E-46 |
| MS.gene05364 | MS.gene72008 | 0.804078 | 2.57E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene72008 | 0.801114 | 1.05E-48 | -1.69E-46 |
| MS.gene053883 | MS.gene72008 | 0.828195 | 1.03E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene72008 | 0.803628 | 3.18E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene72008 | 0.824853 | 6.44E-54 | -1.69E-46 |
| MS.gene054236 | MS.gene72008 | 0.829148 | 6.05E-55 | -1.69E-46 |
| MS.gene05423 | MS.gene72008 | 0.850782 | 1.36E-60 | -1.69E-46 |
| MS.gene054291 | MS.gene72008 | -0.813671 | 2.27E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene72008 | 0.826703 | 2.34E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene72008 | 0.873782 | 1.13E-67 | -1.69E-46 |
| MS.gene054775 | MS.gene72008 | 0.841973 | 3.43E-58 | -1.69E-46 |
| MS.gene054967 | MS.gene72008 | 0.806136 | 9.52E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene72008 | 0.818991 | 1.47E-52 | -1.69E-46 |
| MS.gene055166 | MS.gene72008 | 0.810265 | 1.26E-50 | -1.69E-46 |
| MS.gene055222 | MS.gene72008 | 0.802042 | 6.77E-49 | -1.69E-46 |
| MS.gene055227 | MS.gene72008 | 0.805092 | 1.58E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene72008 | 0.820153 | 7.97E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene72008 | 0.828876 | 7.05E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene72008 | 0.857151 | 1.98E-62 | -1.69E-46 |
| MS.gene055468 | MS.gene72008 | 0.823195 | 1.58E-53 | -1.69E-46 |
| MS.gene055512 | MS.gene72008 | 0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene72008 | 0.808765 | 2.64E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene72008 | -0.818583 | 1.82E-52 | -1.69E-46 |
| MS.gene055586 | MS.gene72008 | 0.813424 | 2.58E-51 | -1.69E-46 |
| MS.gene055589 | MS.gene72008 | 0.815581 | 8.59E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene72008 | 0.834665 | 2.63E-56 | -1.69E-46 |
| MS.gene055808 | MS.gene72008 | 0.814782 | 1.29E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene72008 | 0.834192 | 3.45E-56 | -1.69E-46 |
| MS.gene056050 | MS.gene72008 | 0.823771 | 1.16E-53 | -1.69E-46 |
| MS.gene056173 | MS.gene72008 | 0.801787 | 7.64E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene72008 | 0.830209 | 3.34E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene72008 | 0.840272 | 9.61E-58 | -1.69E-46 |
| MS.gene056308 | MS.gene72008 | 0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene72008 | 0.84305 | 1.78E-58 | -1.69E-46 |
| MS.gene056595 | MS.gene72008 | 0.801576 | 8.44E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene72008 | 0.82306 | 1.70E-53 | -1.69E-46 |
| MS.gene056948 | MS.gene72008 | 0.809192 | 2.14E-50 | -1.69E-46 |
| MS.gene057002 | MS.gene72008 | 0.809062 | 2.28E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene72008 | 0.827157 | 1.83E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene72008 | 0.811463 | 6.92E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene72008 | 0.814019 | 1.91E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene72008 | 0.816432 | 5.55E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene72008 | 0.816781 | 4.63E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene72008 | 0.826698 | 2.35E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene72008 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene72008 | 0.848133 | 7.44E-60 | -1.69E-46 |
| MS.gene058625 | MS.gene72008 | 0.870726 | 1.17E-66 | -1.69E-46 |
| MS.gene058979 | MS.gene72008 | 0.822125 | 2.80E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene72008 | 0.805601 | 1.23E-49 | -1.69E-46 |
| MS.gene059084 | MS.gene72008 | 0.816618 | 5.04E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene72008 | 0.803231 | 3.85E-49 | -1.69E-46 |
| MS.gene05950 | MS.gene72008 | 0.827347 | 1.65E-54 | -1.69E-46 |
| MS.gene05953 | MS.gene72008 | 0.815509 | 8.91E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene72008 | 0.862321 | 5.51E-64 | -1.69E-46 |
| MS.gene059584 | MS.gene72008 | 0.808516 | 2.98E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene72008 | 0.854411 | 1.25E-61 | -1.69E-46 |
| MS.gene059842 | MS.gene72008 | 0.807006 | 6.24E-50 | -1.69E-46 |
| MS.gene059935 | MS.gene72008 | 0.801542 | 8.57E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene72008 | 0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene72008 | 0.835135 | 2.00E-56 | -1.69E-46 |
| MS.gene060519 | MS.gene72008 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene060521 | MS.gene72008 | 0.844602 | 6.83E-59 | -1.69E-46 |
| MS.gene060587 | MS.gene72008 | 0.834083 | 3.68E-56 | -1.69E-46 |
| MS.gene060634 | MS.gene72008 | -0.829104 | 6.21E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene72008 | 0.809819 | 1.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72008.t1 | MTR_4g099300 | 98.684 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 8.74e-44 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72008.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1641 | 1714 | 6.74e-28 | 105 |
| MS.gene72008.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1642 | 1715 | 6.81e-28 | 105 |
| MS.gene72008.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1649 | 1722 | 7.01e-28 | 105 |
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCAGGTGTTTAAAGCATC+AGG | 0.355325 | 8.1:-22949833 | MS.gene72008:intron |
| AGAAAGGGATGATCTTCTTC+TGG | 0.363171 | 8.1:-22950076 | MS.gene72008:CDS |
| CATACCCAATCTTGCTGGTT+TGG | 0.366415 | 8.1:-22949965 | MS.gene72008:CDS |
| GACCGCATACCCAATCTTGC+TGG | 0.373769 | 8.1:-22949970 | MS.gene72008:CDS |
| ACAAACAGTTCCAACTAATC+AGG | 0.386510 | 8.1:-22949778 | MS.gene72008:CDS |
| ATCTTCTTGATTGCAAAACT+TGG | 0.400232 | 8.1:+22950052 | None:intergenic |
| ATCATGAAAACCTGATTAGT+TGG | 0.403453 | 8.1:+22949768 | None:intergenic |
| ACGTGCCAAACCAGCAAGAT+TGG | 0.437473 | 8.1:+22949960 | None:intergenic |
| TCAACATGACAGAGATAGAA+AGG | 0.467247 | 8.1:-22950092 | None:intergenic |
| CGTGCCAAACCAGCAAGATT+GGG | 0.501211 | 8.1:+22949961 | None:intergenic |
| ATCGTCTCTAGATCAACAAA+AGG | 0.502025 | 8.1:-22950031 | MS.gene72008:CDS |
| GGTGTTTAAAGCATCAGGGC+TGG | 0.579563 | 8.1:-22949828 | MS.gene72008:CDS |
| GGTCAATAAGTGATGCTACA+AGG | 0.590453 | 8.1:+22949989 | None:intergenic |
| CAACATGACAGAGATAGAAA+GGG | 0.597624 | 8.1:-22950091 | None:intergenic |
| AACCAGCAAGATTGGGTATG+CGG | 0.598882 | 8.1:+22949968 | None:intergenic |
| TGCAGGTGTTTAAAGCATCA+GGG | 0.599927 | 8.1:-22949832 | MS.gene72008:intron |
| TGGTTTGGCACGTACGTGTG+AGG | 0.670114 | 8.1:-22949950 | MS.gene72008:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATATAGAAAAGAT+TGG | + | chr8.1:22949957-22949976 | None:intergenic | 10.0% |
| !! | ATAATAATATAGAAAAGATT+GGG | + | chr8.1:22949956-22949975 | None:intergenic | 10.0% |
| ATCTTCTTGATTGCAAAACT+TGG | + | chr8.1:22949807-22949826 | None:intergenic | 30.0% | |
| TTCACTTCTATTGTTAATGC+AGG | - | chr8.1:22950007-22950026 | MS.gene72008:CDS | 30.0% | |
| ! | AAGTTTGTTTGGAGAGATAA+AGG | + | chr8.1:22949989-22950008 | None:intergenic | 30.0% |
| ! | CAATAGAAGTGAAGTTTGTT+TGG | + | chr8.1:22950000-22950019 | None:intergenic | 30.0% |
| ACAAACAGTTCCAACTAATC+AGG | - | chr8.1:22950078-22950097 | MS.gene72008:CDS | 35.0% | |
| ATCGTCTCTAGATCAACAAA+AGG | - | chr8.1:22949825-22949844 | MS.gene72008:CDS | 35.0% | |
| AGAAAGGGATGATCTTCTTC+TGG | - | chr8.1:22949780-22949799 | MS.gene72008:CDS | 40.0% | |
| GGTCAATAAGTGATGCTACA+AGG | + | chr8.1:22949870-22949889 | None:intergenic | 40.0% | |
| ! | ATGCAGGTGTTTAAAGCATC+AGG | - | chr8.1:22950023-22950042 | MS.gene72008:CDS | 40.0% |
| ! | TGCAGGTGTTTAAAGCATCA+GGG | - | chr8.1:22950024-22950043 | MS.gene72008:CDS | 40.0% |
| AACCAGCAAGATTGGGTATG+CGG | + | chr8.1:22949891-22949910 | None:intergenic | 45.0% | |
| ! | CATACCCAATCTTGCTGGTT+TGG | - | chr8.1:22949891-22949910 | MS.gene72008:intron | 45.0% |
| ACGTGCCAAACCAGCAAGAT+TGG | + | chr8.1:22949899-22949918 | None:intergenic | 50.0% | |
| CGTGCCAAACCAGCAAGATT+GGG | + | chr8.1:22949898-22949917 | None:intergenic | 50.0% | |
| ! | GGTGTTTAAAGCATCAGGGC+TGG | - | chr8.1:22950028-22950047 | MS.gene72008:CDS | 50.0% |
| GACCGCATACCCAATCTTGC+TGG | - | chr8.1:22949886-22949905 | MS.gene72008:intron | 55.0% | |
| ! | TGGTTTGGCACGTACGTGTG+AGG | - | chr8.1:22949906-22949925 | MS.gene72008:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 22949769 | 22950109 | 22949769 | ID=MS.gene72008 |
| chr8.1 | mRNA | 22949769 | 22950109 | 22949769 | ID=MS.gene72008.t1;Parent=MS.gene72008 |
| chr8.1 | exon | 22949951 | 22950109 | 22949951 | ID=MS.gene72008.t1.exon1;Parent=MS.gene72008.t1 |
| chr8.1 | CDS | 22949951 | 22950109 | 22949951 | ID=cds.MS.gene72008.t1;Parent=MS.gene72008.t1 |
| chr8.1 | exon | 22949769 | 22949849 | 22949769 | ID=MS.gene72008.t1.exon2;Parent=MS.gene72008.t1 |
| chr8.1 | CDS | 22949769 | 22949849 | 22949769 | ID=cds.MS.gene72008.t1;Parent=MS.gene72008.t1 |
| Gene Sequence |
| Protein sequence |
