AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82568


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82568.t1 MTR_6g084440 99.526 211 1 0 1 211 313 523 1.78e-156 446
MS.gene82568.t1 MTR_6g084440 98.578 211 1 1 1 211 313 521 2.53e-153 438
MS.gene82568.t1 MTR_8g104270 75.814 215 46 3 2 211 268 481 2.08e-103 309
MS.gene82568.t1 MTR_4g005350 40.722 194 99 5 1 180 129 320 6.03e-35 128
MS.gene82568.t1 MTR_7g010160 35.583 163 98 4 51 208 496 656 3.54e-19 85.5
MS.gene82568.t1 MTR_5g040600 30.220 182 102 6 12 179 622 792 1.42e-16 78.2
MS.gene82568.t1 MTR_8g467380 30.435 161 105 3 53 208 587 745 6.68e-14 70.5
MS.gene82568.t1 MTR_8g467390 29.008 131 88 3 53 179 206 335 1.60e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82568.t1 AT1G73850 63.063 222 65 4 1 205 325 546 3.96e-87 270
MS.gene82568.t1 AT1G73850 63.063 222 65 4 1 205 325 546 3.96e-87 270
MS.gene82568.t1 AT3G20260 39.698 199 99 6 1 181 131 326 3.04e-31 118
MS.gene82568.t1 AT5G39785 39.259 135 71 4 53 178 349 481 4.31e-18 82.4
MS.gene82568.t1 AT5G39785 39.259 135 71 4 53 178 349 481 4.31e-18 82.4
MS.gene82568.t1 AT5G39785 39.259 135 71 4 53 178 349 481 4.31e-18 82.4
MS.gene82568.t1 AT5G39785 39.259 135 71 4 53 178 349 481 4.44e-18 82.4
MS.gene82568.t1 AT5G39785 39.259 135 71 4 53 178 349 481 4.89e-18 82.4
MS.gene82568.t1 AT1G69610 33.846 130 82 3 53 180 346 473 1.24e-15 75.5
MS.gene82568.t1 AT1G69610 33.846 130 82 3 53 180 391 518 1.62e-15 75.1
MS.gene82568.t1 AT1G69610 33.846 130 82 3 53 180 390 517 1.64e-15 75.1

Find 40 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACATGGGAAGCACTTAAT+TGG 0.198782 6.3:+78741403 MS.gene82568:CDS
ATTCTTGTACCTCGATATTC+AGG 0.218566 6.3:-78741615 None:intergenic
GAGAATGAACCTTATGAATT+TGG 0.221238 6.3:+78741534 MS.gene82568:CDS
TCAGGGACTAGGAGCAATTT+TGG 0.222327 6.3:-78741597 None:intergenic
AGCTTCATTTCTTGTGATAA+TGG 0.291025 6.3:+78742305 MS.gene82568:CDS
GATGATCAGAAGGAGAATAT+TGG 0.307291 6.3:+78742261 MS.gene82568:CDS
GTAGCTCCAAGGTCAATTTC+AGG 0.324497 6.3:+78741279 MS.gene82568:CDS
GCAGCCTCCAATTCACAATA+TGG 0.342594 6.3:-78741360 None:intergenic
ATGATCAGAAGGAGAATATT+GGG 0.345161 6.3:+78742262 MS.gene82568:CDS
GGATTGTGACTTATATCTTC+TGG 0.349354 6.3:-78741339 None:intergenic
ATCAACATTTGATGCTCGTT+TGG 0.350427 6.3:-78741445 None:intergenic
AACTGTTGCGCAATTGTTGC+TGG 0.354425 6.3:-78741477 None:intergenic
GCACAAAAGCTTCATGAAAC+TGG 0.382594 6.3:+78740563 MS.gene82568:CDS
TCACAATCCATATTGTGAAT+TGG 0.401197 6.3:+78741353 MS.gene82568:CDS
AAGAAATGTCATTTCTAGAA+AGG 0.415445 6.3:+78740534 None:intergenic
TTTAAAAGACGGATTAATGT+TGG 0.441489 6.3:-78742432 None:intergenic
TTTAAAGAAAGTTAATCAAA+AGG 0.445739 6.3:+78742449 MS.gene82568:CDS
TTTCTTGTGATAATGGAAGA+TGG 0.449843 6.3:+78742312 MS.gene82568:CDS
CTCATTCTAGCATAAATCTC+TGG 0.472752 6.3:-78741564 None:intergenic
TTGCACAAATTTGCTTAACA+TGG 0.498853 6.3:+78741388 MS.gene82568:CDS
ATTCTCCCTGAAATTGACCT+TGG 0.509048 6.3:-78741285 None:intergenic
GGTCTCCTACCAAATTCATA+AGG 0.517370 6.3:-78741543 None:intergenic
GATTAACTTTCTTTAAAAGA+CGG 0.520863 6.3:-78742443 None:intergenic
CAATCCATATTGTGAATTGG+AGG 0.524173 6.3:+78741356 MS.gene82568:CDS
TCGTAGAAATCAAAGAGGCT+TGG 0.527566 6.3:+78742404 MS.gene82568:CDS
TTATGCTAGAATGAGACATA+TGG 0.527586 6.3:+78741572 MS.gene82568:CDS
TAGCTCCAAGGTCAATTTCA+GGG 0.532390 6.3:+78741280 MS.gene82568:CDS
TTCTTGTACCTCGATATTCA+GGG 0.536989 6.3:-78741614 None:intergenic
ATGAACCTTATGAATTTGGT+AGG 0.537040 6.3:+78741538 MS.gene82568:CDS
AGAATCAGATGATGATCAGA+AGG 0.565291 6.3:+78742251 MS.gene82568:intron
AACATTCATGAATTTCCTCA+AGG 0.579977 6.3:+78742341 MS.gene82568:CDS
CGAGCATCAAATGTTGATGT+TGG 0.585634 6.3:+78741450 MS.gene82568:CDS
TGCACAAATTTGCTTAACAT+GGG 0.586140 6.3:+78741389 MS.gene82568:CDS
GTTTCTCTTTATCAGCCTTG+AGG 0.587313 6.3:-78742356 None:intergenic
TTCTACGAAAGTAAGATGCA+AGG 0.609967 6.3:-78742389 None:intergenic
TACTTTCGTAGAAATCAAAG+AGG 0.610424 6.3:+78742399 MS.gene82568:CDS
TACCTCGATATTCAGGGACT+AGG 0.612708 6.3:-78741608 None:intergenic
GATCTATCAAAGTAGCTCCA+AGG 0.637964 6.3:+78741268 MS.gene82568:CDS
CTCCTAGTCCCTGAATATCG+AGG 0.672700 6.3:+78741606 MS.gene82568:CDS
TAAGATGCAAGGATCTGACA+TGG 0.678566 6.3:-78742378 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTACTTAGATTATTAT+AGG + chr6.3:78741714-78741733 MS.gene82568:intron 10.0%
!! AAATATCAGAAAATTAAGAA+AGG - chr6.3:78740600-78740619 None:intergenic 15.0%
!! GAAGAAATAATATTATAATG+TGG - chr6.3:78740947-78740966 None:intergenic 15.0%
!! TCTTCAAATATATTTGTATT+TGG + chr6.3:78740962-78740981 MS.gene82568:intron 15.0%
!! TTTAAAGAAAGTTAATCAAA+AGG + chr6.3:78742449-78742468 MS.gene82568:CDS 15.0%
!! AAAAATTGTAACTAAGTAGT+TGG + chr6.3:78741058-78741077 MS.gene82568:intron 20.0%
!! AGCATTATTAAACAATTTGT+AGG + chr6.3:78741977-78741996 MS.gene82568:intron 20.0%
!! ATTGCAAAACTATTTAATTG+TGG - chr6.3:78741891-78741910 None:intergenic 20.0%
!! CATTATTAAACAATTTGTAG+GGG + chr6.3:78741979-78741998 MS.gene82568:intron 20.0%
!! GCATTATTAAACAATTTGTA+GGG + chr6.3:78741978-78741997 MS.gene82568:intron 20.0%
!!! ACAATTAAATAGTTTTGCAA+TGG + chr6.3:78741890-78741909 MS.gene82568:intron 20.0%
!!! GATTAACTTTCTTTAAAAGA+CGG - chr6.3:78742446-78742465 None:intergenic 20.0%
! CTAACGTTCAAGATATATTA+TGG + chr6.3:78740692-78740711 MS.gene82568:intron 25.0%
! TCAATAATAGAGAATTTGAC+CGG - chr6.3:78740666-78740685 None:intergenic 25.0%
!!! TAGATATGTGGTTTTTTGTT+TGG + chr6.3:78741831-78741850 MS.gene82568:intron 25.0%
!!! TTTAAAAGACGGATTAATGT+TGG - chr6.3:78742435-78742454 None:intergenic 25.0%
AACATTCATGAATTTCCTCA+AGG + chr6.3:78742341-78742360 MS.gene82568:CDS 30.0%
AGCTTCATTTCTTGTGATAA+TGG + chr6.3:78742305-78742324 MS.gene82568:CDS 30.0%
ATATCTAGCAATCATGTTCT+TGG - chr6.3:78741818-78741837 None:intergenic 30.0%
ATGAACCTTATGAATTTGGT+AGG + chr6.3:78741538-78741557 MS.gene82568:CDS 30.0%
ATGATCAGAAGGAGAATATT+GGG + chr6.3:78742262-78742281 MS.gene82568:CDS 30.0%
GAGAATGAACCTTATGAATT+TGG + chr6.3:78741534-78741553 MS.gene82568:CDS 30.0%
TAATAATGCTTCACACTGAT+GGG - chr6.3:78741967-78741986 None:intergenic 30.0%
TACTTTCGTAGAAATCAAAG+AGG + chr6.3:78742399-78742418 MS.gene82568:CDS 30.0%
TCACAATCCATATTGTGAAT+TGG + chr6.3:78741353-78741372 MS.gene82568:CDS 30.0%
TTAATAATGCTTCACACTGA+TGG - chr6.3:78741968-78741987 None:intergenic 30.0%
TTATGCTAGAATGAGACATA+TGG + chr6.3:78741572-78741591 MS.gene82568:CDS 30.0%
TTTATATATTGCCAACCCAT+TGG + chr6.3:78741923-78741942 MS.gene82568:intron 30.0%
! TGCACAAATTTGCTTAACAT+GGG + chr6.3:78741389-78741408 MS.gene82568:CDS 30.0%
! TTGCACAAATTTGCTTAACA+TGG + chr6.3:78741388-78741407 MS.gene82568:CDS 30.0%
!! TTTCTTGTGATAATGGAAGA+TGG + chr6.3:78742312-78742331 MS.gene82568:CDS 30.0%
!! TTTTTGTTTGGAGGAAAAGA+TGG + chr6.3:78741843-78741862 MS.gene82568:intron 30.0%
!!! ATATGTGGTTTTTTGTTTGG+AGG + chr6.3:78741834-78741853 MS.gene82568:intron 30.0%
AATAATGCTTCACACTGATG+GGG - chr6.3:78741966-78741985 None:intergenic 35.0%
ACTTAAGAACGAAACTTCAG+GGG + chr6.3:78741103-78741122 MS.gene82568:intron 35.0%
AGAATCAGATGATGATCAGA+AGG + chr6.3:78742251-78742270 MS.gene82568:intron 35.0%
ATCAACATTTGATGCTCGTT+TGG - chr6.3:78741448-78741467 None:intergenic 35.0%
ATTCTTGTACCTCGATATTC+AGG - chr6.3:78741618-78741637 None:intergenic 35.0%
CAATCCATATTGTGAATTGG+AGG + chr6.3:78741356-78741375 MS.gene82568:CDS 35.0%
CACTTAAGAACGAAACTTCA+GGG + chr6.3:78741102-78741121 MS.gene82568:intron 35.0%
CTCATTCTAGCATAAATCTC+TGG - chr6.3:78741567-78741586 None:intergenic 35.0%
GAACATGATTGCTAGATATG+TGG + chr6.3:78741819-78741838 MS.gene82568:intron 35.0%
GATGATCAGAAGGAGAATAT+TGG + chr6.3:78742261-78742280 MS.gene82568:CDS 35.0%
GGATTGTGACTTATATCTTC+TGG - chr6.3:78741342-78741361 None:intergenic 35.0%
TCACTTAAGAACGAAACTTC+AGG + chr6.3:78741101-78741120 MS.gene82568:intron 35.0%
TTAATAAGCCAAATCTCTCG+CGG + chr6.3:78740631-78740650 MS.gene82568:intron 35.0%
TTCAACAACCGAAGTTTCAT+TGG - chr6.3:78740845-78740864 None:intergenic 35.0%
TTCTACGAAAGTAAGATGCA+AGG - chr6.3:78742392-78742411 None:intergenic 35.0%
TTCTTGTACCTCGATATTCA+GGG - chr6.3:78741617-78741636 None:intergenic 35.0%
TTGTCTCTCCAATGAAACTT+CGG + chr6.3:78740834-78740853 MS.gene82568:intron 35.0%
! TGTCGACAAGTTGAACATTA+TGG - chr6.3:78740800-78740819 None:intergenic 35.0%
! TTAATCCAACGACTGAGTTT+TGG + chr6.3:78740722-78740741 MS.gene82568:intron 35.0%
!! TAACATGGGAAGCACTTAAT+TGG + chr6.3:78741403-78741422 MS.gene82568:CDS 35.0%
!!! TTGAGAGTGTTTTATCTCGA+CGG + chr6.3:78740877-78740896 MS.gene82568:intron 35.0%
ATTCTCCCTGAAATTGACCT+TGG - chr6.3:78741288-78741307 None:intergenic 40.0%
CGAGCATCAAATGTTGATGT+TGG + chr6.3:78741450-78741469 MS.gene82568:CDS 40.0%
GATCTATCAAAGTAGCTCCA+AGG + chr6.3:78741268-78741287 MS.gene82568:CDS 40.0%
GCACAAAAGCTTCATGAAAC+TGG + chr6.3:78740563-78740582 MS.gene82568:CDS 40.0%
GGTCTCCTACCAAATTCATA+AGG - chr6.3:78741546-78741565 None:intergenic 40.0%
GTTTCTCTTTATCAGCCTTG+AGG - chr6.3:78742359-78742378 None:intergenic 40.0%
TAAGATGCAAGGATCTGACA+TGG - chr6.3:78742381-78742400 None:intergenic 40.0%
TAGCTCCAAGGTCAATTTCA+GGG + chr6.3:78741280-78741299 MS.gene82568:CDS 40.0%
TCGTAGAAATCAAAGAGGCT+TGG + chr6.3:78742404-78742423 MS.gene82568:CDS 40.0%
! ATATTGCCAACCCATTGGTT+AGG + chr6.3:78741928-78741947 MS.gene82568:intron 40.0%
! TATTGCCAACCCATTGGTTA+GGG + chr6.3:78741929-78741948 MS.gene82568:intron 40.0%
AACTGTTGCGCAATTGTTGC+TGG - chr6.3:78741480-78741499 None:intergenic 45.0%
ATGCATGGACCCTAACCAAT+GGG - chr6.3:78741941-78741960 None:intergenic 45.0%
GAGAATTTGACCGGAGATTG+AGG - chr6.3:78740657-78740676 None:intergenic 45.0%
GCAGCCTCCAATTCACAATA+TGG - chr6.3:78741363-78741382 None:intergenic 45.0%
GTAGCTCCAAGGTCAATTTC+AGG + chr6.3:78741279-78741298 MS.gene82568:CDS 45.0%
TACCTCGATATTCAGGGACT+AGG - chr6.3:78741611-78741630 None:intergenic 45.0%
TATGCATGGACCCTAACCAA+TGG - chr6.3:78741942-78741961 None:intergenic 45.0%
TATTGCCTCTCGAGTTTCTC+CGG - chr6.3:78740748-78740767 None:intergenic 45.0%
! TCAGGGACTAGGAGCAATTT+TGG - chr6.3:78741600-78741619 None:intergenic 45.0%
!! ACTAAGTAGTTGGCTTGAGC+TGG + chr6.3:78741068-78741087 MS.gene82568:intron 45.0%
!! AAAAAAATTAGTTATTATTA+AGG + chr6.3:78741144-78741163 MS.gene82568:intron 5.0%
ATGGACCCTAACCAATGGGT+TGG - chr6.3:78741937-78741956 None:intergenic 50.0%
CTCCTAGTCCCTGAATATCG+AGG + chr6.3:78741606-78741625 MS.gene82568:CDS 50.0%
!! TCCAACGACTGAGTTTTGGC+CGG + chr6.3:78740726-78740745 MS.gene82568:intron 50.0%
CACACTGATGGGGCTATGCA+TGG - chr6.3:78741956-78741975 None:intergenic 55.0%
GATTGAGGCCGCGAGAGATT+TGG - chr6.3:78740642-78740661 None:intergenic 55.0%
TCCGGCCAAAACTCAGTCGT+TGG - chr6.3:78740730-78740749 None:intergenic 55.0%
TTTGGCCGGAGAAACTCGAG+AGG + chr6.3:78740740-78740759 MS.gene82568:intron 55.0%
TCTCTCGCGGCCTCAATCTC+CGG + chr6.3:78740644-78740663 MS.gene82568:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 78740539 78742482 78740539 ID=MS.gene82568
chr6.3 mRNA 78740539 78742482 78740539 ID=MS.gene82568.t1;Parent=MS.gene82568
chr6.3 exon 78740539 78740584 78740539 ID=MS.gene82568.t1.exon1;Parent=MS.gene82568.t1
chr6.3 CDS 78740539 78740584 78740539 ID=cds.MS.gene82568.t1;Parent=MS.gene82568.t1
chr6.3 exon 78741259 78741627 78741259 ID=MS.gene82568.t1.exon2;Parent=MS.gene82568.t1
chr6.3 CDS 78741259 78741627 78741259 ID=cds.MS.gene82568.t1;Parent=MS.gene82568.t1
chr6.3 exon 78742253 78742482 78742253 ID=MS.gene82568.t1.exon3;Parent=MS.gene82568.t1
chr6.3 CDS 78742253 78742482 78742253 ID=cds.MS.gene82568.t1;Parent=MS.gene82568.t1
Gene Sequence

>MS.gene82568

ATGTCATTTCTAGAAAGGATTAGTGCACAAAAGCTTCATGAAACTGGTAACATATACACCTTTCTTAATTTTCTGATATTTAATTGCTCAAATTAATAAGCCAAATCTCTCGCGGCCTCAATCTCCGGTCAAATTCTCTATTATTGAATTAATCTAACGTTCAAGATATATTATGGAGTTAGATTAATCCAACGACTGAGTTTTGGCCGGAGAAACTCGAGAGGCAATAAAAGATTCAAGTTGTTTAATAAGTATATACCATAATGTTCAACTTGTCGACAATTTGAATCTTCTCTTGTCTCTCCAATGAAACTTCGGTTGTTGAATTAATCTAATAGTTGAGAGTGTTTTATCTCGACGGTTAGATTAATTCAATTGCTAACACGAAATATTTCATAATAATAGCCACATTATAATATTATTTCTTCAAATATATTTGTATTTGGAAACTTTTTCTTTTTGTTGCTTAAAATATTTGTGTTTTGAAACAATAATAAAAAATAGTTTTAATCAAATTCAAAAAATTGTAACTAAGTAGTTGGCTTGAGCTGGTTAACAAGCTTCACTTAAGAACGAAACTTCAGGGGTAAAGATAAAAAAAAAAAAAAAAAATTAGTTATTATTAAGGATTGATTTTGTTAGACATTATTAGTGACACTTTTATTGTTAAGAGTTGATTTTGTGACACATTATTATTAGTAACACTTAAATTAATTTCAGAATCATTGAGATCTATCAAAGTAGCTCCAAGGTCAATTTCAGGGAGAATTGTGTACAAACTTTCAAGTATGAACAAAAAACCAGAAGATATAAGTCACAATCCATATTGTGAATTGGAGGCTGCATATGTTGCACAAATTTGCTTAACATGGGAAGCACTTAATTGGAACTACAAGAATTTTCAAACCAAACGAGCATCAAATGTTGATGTTGGTTGTCCAGCAACAATTGCGCAACAGTTTCAACAATTTCAAGTTTTGTTGCAAAGATATGTAGAGAATGAACCTTATGAATTTGGTAGGAGACCAGAGATTTATGCTAGAATGAGACATATGGCACCAAAATTGCTCCTAGTCCCTGAATATCGAGGTACAAGAATTATTTTATTATCAAATTTTTTAAAAATAAATTATTTAAATTTTATACAATTCATTTTTTATATGATTTTAAATTAATTTATTACTTAGATTATTATAGGTTACAGAACAAATATTTAAATGTTACTTATTAAAAAAAATAAAACAAAATTTATCACAGATTCTTTTATAAATCATGACCAAGAACATGATTGCTAGATATGTGGTTTTTTGTTTGGAGGAAAAGATGGTGTAAGTTTTAAAATGTATATTCCACAATTAAATAGTTTTGCAATGGTGTATTTCTTTTTATATATTGCCAACCCATTGGTTAGGGTCCATGCATAGCCCCATCAGTGTGAAGCATTATTAAACAATTTGTAGGGGAATTAAAGAGTATATAAATATAATTAATTGAATATTTAAAATCAATTTTAACAAAGACTTTTTTATATTTTTATTTCTCACTTAATTATATTTTAATTTTTTATTCATAGTGTTTAGTGTACAAATAATATTGTATTCATACAAAATTTACTTGCTGTGATATTAATTATGACTATATTTATTTTTTTCTTGTTTGTGTTAATTAACTAAAGAATCAGTATTTTTTTCTTGTTTGTGTTAATTAACTAAAGAATCAGATGATGATCAGAAGGAGAATATTGGGTTTAACACTAAAATATCATCAGCTTCATTTCTTGTGATAATGGAAGATGGAATCAGAACATTCATGAATTTCCTCAAGGCTGATAAAGAGAAACCATGTCAGATCCTTGCATCTTACTTTCGTAGAAATCAAAGAGGCTTGGTTGATCCAACATTAATCCGTCTTTTAAAGAAAGTTAATCAAAAGGCAAGTTCCTAA

Protein sequence

>MS.gene82568.t1

MSFLERISAQKLHETESLRSIKVAPRSISGRIVYKLSSMNKKPEDISHNPYCELEAAYVAQICLTWEALNWNYKNFQTKRASNVDVGCPATIAQQFQQFQVLLQRYVENEPYEFGRRPEIYARMRHMAPKLLLVPEYRESDDDQKENIGFNTKISSASFLVIMEDGIRTFMNFLKADKEKPCQILASYFRRNQRGLVDPTLIRLLKKVNQKASS