AlfalfaGEDB | MS.gene88233

M. sativa cultivar XinJiangDaYe / MS.gene88233

KEGG: K02326
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88233.t1	APC23631.1	100	81	0	0	21	101	54	134	1.30E-33	152.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88233.t1	A0A1J0F5C9	100.0	81	0	0	21	101	54	134	9.7e-34	152.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene88233	MS.gene89203	PPI
MS.gene88233	MS.gene52605	PPI
MS.gene88233	MS.gene00233	PPI
MS.gene88233	MS.gene068178	PPI
MS.gene58285	MS.gene88233	PPI
MS.gene88233	MS.gene47573	PPI
MS.gene88233	MS.gene41343	PPI
MS.gene88233	MS.gene027904	PPI
MS.gene88233	MS.gene53708	PPI
MS.gene88233	MS.gene059054	PPI
MS.gene027907	MS.gene88233	PPI
MS.gene88233	MS.gene26084	PPI
MS.gene88233	MS.gene017431	PPI
MS.gene88233	MS.gene78227	PPI
MS.gene88233	MS.gene70258	PPI
MS.gene58116	MS.gene88233	PPI
MS.gene88233	MS.gene65125	PPI
MS.gene88233	MS.gene021887	PPI
MS.gene88233	MS.gene23886	PPI
MS.gene28432	MS.gene88233	PPI
MS.gene88233	MS.gene015760	PPI
MS.gene88233	MS.gene29445	PPI
MS.gene88233	MS.gene78224	PPI
MS.gene88233	MS.gene038612	PPI
MS.gene78924	MS.gene88233	PPI
MS.gene88233	MS.gene033505	PPI
MS.gene39538	MS.gene88233	PPI
MS.gene78922	MS.gene88233	PPI
MS.gene071851	MS.gene88233	PPI
MS.gene88233	MS.gene28438	PPI
MS.gene28434	MS.gene88233	PPI
MS.gene88233	MS.gene055778	PPI
MS.gene97701	MS.gene88233	PPI
MS.gene88233	MS.gene91631	PPI
MS.gene88233	MS.gene04748	PPI
MS.gene88233	MS.gene030661	PPI
MS.gene88233	MS.gene26406	PPI
MS.gene028868	MS.gene88233	PPI
MS.gene88233	MS.gene70990	PPI
MS.gene88233	MS.gene068179	PPI
MS.gene88233	MS.gene027909	PPI
MS.gene88233	MS.gene70261	PPI
MS.gene88233	MS.gene009155	PPI
MS.gene000365	MS.gene88233	PPI
MS.gene88233	MS.gene97703	PPI
MS.gene049111	MS.gene88233	PPI
MS.gene88233	MS.gene58114	PPI
MS.gene88233	MS.gene030664	PPI
MS.gene70263	MS.gene88233	PPI
MS.gene88233	MS.gene030666	PPI
MS.gene88233	MS.gene043455	PPI
MS.gene88233	MS.gene05905	PPI
MS.gene88233	MS.gene024064	PPI
MS.gene88233	MS.gene31434	PPI
MS.gene88233	MS.gene64164	PPI
MS.gene88233	MS.gene43369	PPI
MS.gene66051	MS.gene88233	PPI
MS.gene88233	MS.gene057458	PPI
MS.gene88233	MS.gene056335	PPI
MS.gene88233	MS.gene58112	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88233.t1	MTR_4g052950	77.885	104	8	2	4	101	46	140	4.43e-48	150

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88233.t1	AT2G27470	47.541	61	23	1	4	64	50	101	7.55e-12	59.7

Find 20 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCATCTCCCTTTCCTTT+TGG	0.216918	4.4:-49257193	None:intergenic
GAAGGCGAGCCGTCTGATAA+AGG	0.396478	4.4:+49257235	MS.gene88233:CDS
CAGAGTTTACTGAGTTTGTT+GGG	0.413182	4.4:+49256434	MS.gene88233:CDS
CCTCTAAAAGATTCTCTTGA+AGG	0.437256	4.4:+49256457	MS.gene88233:CDS
ACAGAGTTTACTGAGTTTGT+TGG	0.451694	4.4:+49256433	MS.gene88233:CDS
AGCGGCAGTGCCAAAAGGAA+AGG	0.481373	4.4:+49257183	MS.gene88233:CDS
GCAGGGCTAATGATATATGT+AGG	0.482928	4.4:+49256356	MS.gene88233:intron
TGAGAAGGGAAAGAAAAGAA+AGG	0.540607	4.4:+49257210	MS.gene88233:CDS
AAAAGGAAAGGGAGATGAGA+AGG	0.549945	4.4:+49257195	MS.gene88233:CDS
CAGGGCTAATGATATATGTA+GGG	0.556080	4.4:+49256357	MS.gene88233:intron
AAGAAAGCGGCAGTGCCAAA+AGG	0.559929	4.4:+49257178	MS.gene88233:CDS
GCGGCAGTGCCAAAAGGAAA+GGG	0.560436	4.4:+49257184	MS.gene88233:CDS
CCGTCTGATAAAGGCGATGG+TGG	0.589063	4.4:+49257244	MS.gene88233:CDS
AAAGGAAAGGGAGATGAGAA+GGG	0.589156	4.4:+49257196	MS.gene88233:CDS
GGAAAGAAAAGAAAGGCGGA+AGG	0.603937	4.4:+49257217	MS.gene88233:CDS
GAAGGGAAAGAAAAGAAAGG+CGG	0.609576	4.4:+49257213	MS.gene88233:CDS
CCACCATCGCCTTTATCAGA+CGG	0.623150	4.4:-49257244	None:intergenic
GAGCCGTCTGATAAAGGCGA+TGG	0.623654	4.4:+49257241	MS.gene88233:CDS
AAAGAATGCTGGGAAGAAAG+CGG	0.668115	4.4:+49257165	MS.gene88233:CDS
ATATATGTAGGGAGTCAAAG+AGG	0.682246	4.4:+49256368	MS.gene88233:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTTCTTTTCATTGCAA+TGG	+	chr4.4:49256625-49256644	MS.gene88233:intron	20.0%
!!	ATAAAAATGGGAACTTTTTG+AGG	+	chr4.4:49256294-49256313	MS.gene88233:intron	25.0%
!!!	CATTTTAGAGTTACAATTCA+TGG	-	chr4.4:49256764-49256783	None:intergenic	25.0%
	AAGAGGAATGAAACTATTTG+TGG	+	chr4.4:49256959-49256978	MS.gene88233:intron	30.0%
	AATCCCCATTTAGTTGAAAA+TGG	+	chr4.4:49256695-49256714	MS.gene88233:intron	30.0%
	AGAGGAATGAAACTATTTGT+GGG	+	chr4.4:49256960-49256979	MS.gene88233:intron	30.0%
	AGTTTAGGAAAAAGAATGCT+GGG	+	chr4.4:49257155-49257174	MS.gene88233:CDS	30.0%
	ATCCCCATTTAGTTGAAAAT+GGG	+	chr4.4:49256696-49256715	MS.gene88233:intron	30.0%
	TCCTGGGTGATTATAAAAAT+GGG	+	chr4.4:49256282-49256301	MS.gene88233:intron	30.0%
	TGCAATGGAAGTTCAAAAAA+AGG	+	chr4.4:49256640-49256659	MS.gene88233:intron	30.0%
	TTATGCTACATAAACAAGTG+TGG	+	chr4.4:49256898-49256917	MS.gene88233:intron	30.0%
	TTCCTGGGTGATTATAAAAA+TGG	+	chr4.4:49256281-49256300	MS.gene88233:intron	30.0%
!	AACCCATTTTCAACTAAATG+GGG	-	chr4.4:49256701-49256720	None:intergenic	30.0%
!	CAACCCATTTTCAACTAAAT+GGG	-	chr4.4:49256702-49256721	None:intergenic	30.0%
!	GAAAATTTTGCATTGGGTTT+TGG	+	chr4.4:49256209-49256228	MS.gene88233:CDS	30.0%
!	GCATAGAATAACTCAGTTTT+AGG	-	chr4.4:49256804-49256823	None:intergenic	30.0%
!	TCTTTTTCCTAAACTCTGAT+AGG	-	chr4.4:49257150-49257169	None:intergenic	30.0%
!	TGTCATTTGAGCAATCATTA+TGG	+	chr4.4:49256602-49256621	MS.gene88233:intron	30.0%
!	TTTTTGACCTATCAGAGTTT+AGG	+	chr4.4:49257140-49257159	MS.gene88233:intron	30.0%
!!	GTCAAAAAGAAAAAGTGTCA+AGG	-	chr4.4:49257128-49257147	None:intergenic	30.0%
!!	TTGTGTTGTTTGTATTTGCA+GGG	+	chr4.4:49256339-49256358	MS.gene88233:intron	30.0%
!!	TTTGTGTTGTTTGTATTTGC+AGG	+	chr4.4:49256338-49256357	MS.gene88233:intron	30.0%
!!!	CAGTTTTAGGCTTTTAACAA+TGG	-	chr4.4:49256791-49256810	None:intergenic	30.0%
!!!	TGTTATGACTGCTTTGTTTT+CGG	+	chr4.4:49256497-49256516	MS.gene88233:intron	30.0%
	AAACAAAGCAGTCATAACAC+AGG	-	chr4.4:49256496-49256515	None:intergenic	35.0%
	AACAAAGCAGTCATAACACA+GGG	-	chr4.4:49256495-49256514	None:intergenic	35.0%
	AACTGAGTTATTCTATGCTC+TGG	+	chr4.4:49256806-49256825	MS.gene88233:intron	35.0%
	ACAGAGTTTACTGAGTTTGT+TGG	+	chr4.4:49256433-49256452	MS.gene88233:CDS	35.0%
	ATATATGTAGGGAGTCAAAG+AGG	+	chr4.4:49256368-49256387	MS.gene88233:CDS	35.0%
	ATGGAAGATATGATCTTGCT+TGG	+	chr4.4:49256859-49256878	MS.gene88233:intron	35.0%
	CAGGGCTAATGATATATGTA+GGG	+	chr4.4:49256357-49256376	MS.gene88233:intron	35.0%
	CCATTTAGTTGAAAATGGGT+TGG	+	chr4.4:49256700-49256719	MS.gene88233:intron	35.0%
	GAGTTTAGGAAAAAGAATGC+TGG	+	chr4.4:49257154-49257173	MS.gene88233:intron	35.0%
	GCTAGTGAGTAATATTTGGT+TGG	+	chr4.4:49256924-49256943	MS.gene88233:intron	35.0%
	GTCAGCTAGTGAGTAATATT+TGG	+	chr4.4:49256920-49256939	MS.gene88233:intron	35.0%
	TGAGAAGGGAAAGAAAAGAA+AGG	+	chr4.4:49257210-49257229	MS.gene88233:CDS	35.0%
!	CAGAGTTTACTGAGTTTGTT+GGG	+	chr4.4:49256434-49256453	MS.gene88233:CDS	35.0%
!	CCAACCCATTTTCAACTAAA+TGG	-	chr4.4:49256703-49256722	None:intergenic	35.0%
!	CCTCTAAAAGATTCTCTTGA+AGG	+	chr4.4:49256457-49256476	MS.gene88233:CDS	35.0%
!	CCTTCAAGAGAATCTTTTAG+AGG	-	chr4.4:49256460-49256479	None:intergenic	35.0%
!!	AGAGTGAAGTGAAAGTATCA+TGG	+	chr4.4:49256571-49256590	MS.gene88233:intron	35.0%
!!	TCCCATTTTTATAATCACCC+AGG	-	chr4.4:49256286-49256305	None:intergenic	35.0%
!!	TGGCTATTTTGGAGGAAATT+GGG	+	chr4.4:49256229-49256248	MS.gene88233:CDS	35.0%
!!	TTGGCTATTTTGGAGGAAAT+TGG	+	chr4.4:49256228-49256247	MS.gene88233:CDS	35.0%
!!!	AAATTGGGGAAGTTTTGTTG+TGG	+	chr4.4:49256244-49256263	MS.gene88233:intron	35.0%
!!!	AATTGGGGAAGTTTTGTTGT+GGG	+	chr4.4:49256245-49256264	MS.gene88233:intron	35.0%
	AAAAGGAAAGGGAGATGAGA+AGG	+	chr4.4:49257195-49257214	MS.gene88233:CDS	40.0%
	AAAGAATGCTGGGAAGAAAG+CGG	+	chr4.4:49257165-49257184	MS.gene88233:CDS	40.0%
	AAAGGAAAGGGAGATGAGAA+GGG	+	chr4.4:49257196-49257215	MS.gene88233:CDS	40.0%
	GAAGGGAAAGAAAAGAAAGG+CGG	+	chr4.4:49257213-49257232	MS.gene88233:CDS	40.0%
	GCAGGGCTAATGATATATGT+AGG	+	chr4.4:49256356-49256375	MS.gene88233:intron	40.0%
	TCTTCCATCATGCTTAGAGT+TGG	-	chr4.4:49256847-49256866	None:intergenic	40.0%
	TGAACCAACTCTAAGCATGA+TGG	+	chr4.4:49256840-49256859	MS.gene88233:intron	40.0%
!	GTTGGTTCATAGCTACAAAG+AGG	+	chr4.4:49256942-49256961	MS.gene88233:intron	40.0%
!	TTCTCATCTCCCTTTCCTTT+TGG	-	chr4.4:49257196-49257215	None:intergenic	40.0%
!!	GGCTATTTTGGAGGAAATTG+GGG	+	chr4.4:49256230-49256249	MS.gene88233:CDS	40.0%
!!!	GCATTGGGTTTTGGCTATTT+TGG	+	chr4.4:49256218-49256237	MS.gene88233:CDS	40.0%
!!!	GGAAGTTTTGTTGTGGGTTT+TGG	+	chr4.4:49256251-49256270	MS.gene88233:intron	40.0%
!!!	GGTTTTGGAGATTGATTCCT+GGG	+	chr4.4:49256266-49256285	MS.gene88233:intron	40.0%
!!!	TTGGGTTTTGGCTATTTTGG+AGG	+	chr4.4:49256221-49256240	MS.gene88233:CDS	40.0%
	GACCAATATCTGCCCTAACT+TGG	+	chr4.4:49257098-49257117	MS.gene88233:intron	45.0%
	GGAAAGAAAAGAAAGGCGGA+AGG	+	chr4.4:49257217-49257236	MS.gene88233:CDS	45.0%
	TGCCAAGTTAGGGCAGATAT+TGG	-	chr4.4:49257103-49257122	None:intergenic	45.0%
	TGTCAAGGTCTGCCAAGTTA+GGG	-	chr4.4:49257113-49257132	None:intergenic	45.0%
!!!	GGGTTTTGGAGATTGATTCC+TGG	+	chr4.4:49256265-49256284	MS.gene88233:intron	45.0%
	AAGAAAGCGGCAGTGCCAAA+AGG	+	chr4.4:49257178-49257197	MS.gene88233:CDS	50.0%
	CCACCATCGCCTTTATCAGA+CGG	-	chr4.4:49257247-49257266	None:intergenic	50.0%
	GTGTCAAGGTCTGCCAAGTT+AGG	-	chr4.4:49257114-49257133	None:intergenic	50.0%
	AGCGGCAGTGCCAAAAGGAA+AGG	+	chr4.4:49257183-49257202	MS.gene88233:CDS	55.0%
	CCGTCTGATAAAGGCGATGG+TGG	+	chr4.4:49257244-49257263	MS.gene88233:CDS	55.0%
	GAAGGCGAGCCGTCTGATAA+AGG	+	chr4.4:49257235-49257254	MS.gene88233:CDS	55.0%
	GAGCCGTCTGATAAAGGCGA+TGG	+	chr4.4:49257241-49257260	MS.gene88233:CDS	55.0%
	GCGGCAGTGCCAAAAGGAAA+GGG	+	chr4.4:49257184-49257203	MS.gene88233:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	49256204	49257282	49256204	ID=MS.gene88233
chr4.4	mRNA	49256204	49257282	49256204	ID=MS.gene88233.t1;Parent=MS.gene88233
chr4.4	exon	49256204	49256262	49256204	ID=MS.gene88233.t1.exon1;Parent=MS.gene88233.t1
chr4.4	CDS	49256204	49256262	49256204	ID=cds.MS.gene88233.t1;Parent=MS.gene88233.t1
chr4.4	exon	49256360	49256478	49256360	ID=MS.gene88233.t1.exon2;Parent=MS.gene88233.t1
chr4.4	CDS	49256360	49256478	49256360	ID=cds.MS.gene88233.t1;Parent=MS.gene88233.t1
chr4.4	exon	49257155	49257282	49257155	ID=MS.gene88233.t1.exon3;Parent=MS.gene88233.t1
chr4.4	CDS	49257155	49257282	49257155	ID=cds.MS.gene88233.t1;Parent=MS.gene88233.t1

Gene Sequence

>MS.gene88233

ATGGGGAAAATTTTGCATTGGGTTTTGGCTATTTTGGAGGAAATTGGGGAAGTTTTGTTGTGGGTTTTGGAGATTGATTCCTGGGTGATTATAAAAATGGGAACTTTTTGAGGTAGAAGAATGTTAAAGAGTGATTTGTGTTGTTTGTATTTGCAGGGCTAATGATATATGTAGGGAGTCAAAGAGGCAAATCATCAATGCTGAAGATGTGTTCAAAGCTCTTGAAGAAACAGAGTTTACTGAGTTTGTTGGGCCTCTAAAAGATTCTCTTGAAGGTCACTTTTACTTCCCTGTGTTATGACTGCTTTGTTTTCGGATTAGCTGTGATTTATTTTTACTTTTTTGTTAGTATTAATTTGTTAGCTGAAGAGTGAAGTGAAAGTATCATGGTGAAGTGTTGTCATTTGAGCAATCATTATGGTATTTTTCTTTTCATTGCAATGGAAGTTCAAAAAAAGGATTACAATTTTGTGAACTTTAAAATTAGACGTAATCCCCATTTAGTTGAAAATGGGTTGGTTATTATTGTTAGTGAAAAGTTAGAGTAATCGAAGTGTCCATGAATTGTAACTCTAAAATGTCTTCCATTGTTAAAAGCCTAAAACTGAGTTATTCTATGCTCTGGTGTGTACATTGTGAACCAACTCTAAGCATGATGGAAGATATGATCTTGCTTGGTGTTCACAGTTCACTGTTATGCTACATAAACAAGTGTGGTCAGCTAGTGAGTAATATTTGGTTGGTTCATAGCTACAAAGAGGAATGAAACTATTTGTGGGAGTATGTTTTTTGAATTACACATGAAACTACTTCGTAATAATTTGCATGAAAATTTACTTCATTTTATGATTTCATTCACATTAAGAAAAGAGATATGTGTAATTGTTTAGATTTGACCAATATCTGCCCTAACTTGGCAGACCTTGACACTTTTTCTTTTTGACCTATCAGAGTTTAGGAAAAAGAATGCTGGGAAGAAAGCGGCAGTGCCAAAAGGAAAGGGAGATGAGAAGGGAAAGAAAAGAAAGGCGGAAGGCGAGCCGTCTGATAAAGGCGATGGTGGTGATGATGACCAATGA

Protein sequence

>MS.gene88233.t1

MGKILHWVLAILEEIGEVLLANDICRESKRQIINAEDVFKALEETEFTEFVGPLKDSLEEFRKKNAGKKAAVPKGKGDEKGKKRKAEGEPSDKGDGGDDDQ