AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39538


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39538.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene39538.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene39538.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene39538.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene39538.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene39538.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene39538.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene39538.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene39538.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene39538.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene39538.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene39538.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene39538.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene39538.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene39538.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene39538.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene39538.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene39538.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene39538.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene39538.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene39538.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene39538.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene39538.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene39538.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39538.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene39538.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene39538.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene39538.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene39538.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene39538.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene39538.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene39538.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene39538.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene39538.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene39538.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene39538.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene39538.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene39538.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene39538.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene39538.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAACCTCACCGTTTC+AGG 0.224781 4.2:-23184335 MS.gene39538:CDS
TCACGGAGAGCGACGGTTCC+TGG 0.319730 4.2:+23184312 None:intergenic
TGAGGAGCTCAGTGCTCTTC+TGG 0.339681 4.2:+23184277 None:intergenic
AAGCCGACACGGCGGAGCTC+TGG 0.377718 4.2:+23184187 None:intergenic
TTCTTCACTCCGCCGGTGGC+CGG 0.393192 4.2:+23184351 None:intergenic
TTTGTGGCGAGTTGCTTCCT+TGG 0.400731 4.2:+23184393 None:intergenic
GGAAGGGGAGTTTGCGGATG+AGG 0.429699 4.2:+23184259 None:intergenic
TTATCTCGTCGGTCTCTTTG+AGG 0.447224 4.2:-23184145 MS.gene39538:CDS
CTTCCTTGGAGCTTTGCCAC+CGG 0.456245 4.2:+23184407 None:intergenic
CGCAAATCTGCTCCGGCCAC+CGG 0.457456 4.2:-23184363 MS.gene39538:CDS
AGTGACACGCTTAGCATGAA+TGG 0.474744 4.2:+23184107 None:intergenic
CCTTGGAGCTTTGCCACCGG+TGG 0.488901 4.2:+23184410 None:intergenic
CGCTTCTTGCAAAGCCGACA+CGG 0.492590 4.2:+23184176 None:intergenic
CGACGGTTCCTGGCCTGAAA+CGG 0.503357 4.2:+23184322 None:intergenic
GGCTTTGCAAGAAGCGGCCG+AGG 0.504571 4.2:-23184169 MS.gene39538:CDS
GGATTTGCGAGCGGTTTGCT+TGG 0.506530 4.2:+23184431 None:intergenic
CTAGCGAGCTGGATATCCTT+AGG 0.510191 4.2:+23184078 None:intergenic
AAACCTCACCGTTTCAGGCC+AGG 0.512079 4.2:-23184330 MS.gene39538:CDS
CACGGATACGCCTAGCGAGC+TGG 0.513784 4.2:+23184067 None:intergenic
TTTCCAGAGCTCCGCCGTGT+CGG 0.519570 4.2:-23184190 MS.gene39538:CDS
GACGCTGGAAGGGGAGTTTG+CGG 0.521996 4.2:+23184253 None:intergenic
ACACGGCGGAGCTCTGGAAA+CGG 0.525377 4.2:+23184193 None:intergenic
AGCCGCTCGCAAATCTGCTC+CGG 0.526992 4.2:-23184370 MS.gene39538:CDS
CACGGACAAGACGCTGGAAG+GGG 0.529109 4.2:+23184244 None:intergenic
CTCACGGACAAGACGCTGGA+AGG 0.539901 4.2:+23184242 None:intergenic
GTGAGGTTTCTTCACTCCGC+CGG 0.553823 4.2:+23184344 None:intergenic
CTAAGGATATCCAGCTCGCT+AGG 0.554905 4.2:-23184077 MS.gene39538:CDS
GCGTGTCACTATCATGCCTA+AGG 0.557496 4.2:-23184094 MS.gene39538:CDS
TGAAATCCTGAGCGATCTCA+CGG 0.570370 4.2:+23184226 None:intergenic
TCTGGTACTTGCGGATCTCA+CGG 0.571857 4.2:+23184295 None:intergenic
CCACCGGTGGCAAAGCTCCA+AGG 0.572773 4.2:-23184410 MS.gene39538:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 4.2:+23184286 None:intergenic
TCACGGACAAGACGCTGGAA+GGG 0.583031 4.2:+23184243 None:intergenic
AAATCTGCTCCGGCCACCGG+CGG 0.584127 4.2:-23184360 MS.gene39538:CDS
CGATCTCACGGACAAGACGC+TGG 0.585935 4.2:+23184238 None:intergenic
AGGTTTCTTCACTCCGCCGG+TGG 0.590490 4.2:+23184347 None:intergenic
TCTTGTCCGTGAGATCGCTC+AGG 0.603921 4.2:-23184232 MS.gene39538:CDS
GCGGCCGAGGCTTATCTCGT+CGG 0.608232 4.2:-23184156 MS.gene39538:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.613586 4.2:-23184426 MS.gene39538:CDS
ACCGCTCGCAAATCCACCGG+TGG 0.632751 4.2:-23184423 MS.gene39538:CDS
AAGATCAAGCACGCTCGCCA+CGG 0.639365 4.2:+23184049 None:intergenic
GGCCGGAGCAGATTTGCGAG+CGG 0.640066 4.2:+23184368 None:intergenic
GCCACCGGTGGATTTGCGAG+CGG 0.642552 4.2:+23184422 None:intergenic
CGTGTCGGCTTTGCAAGAAG+CGG 0.647453 4.2:-23184175 MS.gene39538:CDS
GAGACCGACGAGATAAGCCT+CGG 0.653052 4.2:+23184152 None:intergenic
GCGGATCTCACGGAGAGCGA+CGG 0.662183 4.2:+23184305 None:intergenic
GTTCCTGGCCTGAAACGGTG+AGG 0.663103 4.2:+23184327 None:intergenic
CAGCTCGCTAGGCGTATCCG+TGG 0.667707 4.2:-23184066 MS.gene39538:CDS
GCTTAGCATGAATGGCGCAG+AGG 0.694649 4.2:+23184115 None:intergenic
TTCTTGCAAAGCCGACACGG+CGG 0.736453 4.2:+23184179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTGACACGCTTAGCATGAA+TGG + chr4.2:23184390-23184409 None:intergenic 45.0%
TGAAATCCTGAGCGATCTCA+CGG + chr4.2:23184271-23184290 None:intergenic 45.0%
TGAAGAAACCTCACCGTTTC+AGG - chr4.2:23184159-23184178 MS.gene39538:CDS 45.0%
TTATCTCGTCGGTCTCTTTG+AGG - chr4.2:23184349-23184368 MS.gene39538:CDS 45.0%
CAGTGCTCTTCTGGTACTTG+CGG + chr4.2:23184211-23184230 None:intergenic 50.0%
CTAAGGATATCCAGCTCGCT+AGG - chr4.2:23184417-23184436 MS.gene39538:CDS 50.0%
CTAGCGAGCTGGATATCCTT+AGG + chr4.2:23184419-23184438 None:intergenic 50.0%
TCTGGTACTTGCGGATCTCA+CGG + chr4.2:23184202-23184221 None:intergenic 50.0%
TTTGTGGCGAGTTGCTTCCT+TGG + chr4.2:23184104-23184123 None:intergenic 50.0%
! AGATTTGCGAGCGGCTTTTG+TGG + chr4.2:23184120-23184139 None:intergenic 50.0%
! GCGTGTCACTATCATGCCTA+AGG - chr4.2:23184400-23184419 MS.gene39538:CDS 50.0%
AAACCTCACCGTTTCAGGCC+AGG - chr4.2:23184164-23184183 MS.gene39538:CDS 55.0%
CAAACCGCTCGCAAATCCAC+CGG - chr4.2:23184068-23184087 MS.gene39538:CDS 55.0%
CGCTTCTTGCAAAGCCGACA+CGG + chr4.2:23184321-23184340 None:intergenic 55.0%
CTTCCTTGGAGCTTTGCCAC+CGG + chr4.2:23184090-23184109 None:intergenic 55.0%
GAGACCGACGAGATAAGCCT+CGG + chr4.2:23184345-23184364 None:intergenic 55.0%
GCTTAGCATGAATGGCGCAG+AGG + chr4.2:23184382-23184401 None:intergenic 55.0%
GTGAGGTTTCTTCACTCCGC+CGG + chr4.2:23184153-23184172 None:intergenic 55.0%
TCACGGACAAGACGCTGGAA+GGG + chr4.2:23184254-23184273 None:intergenic 55.0%
TCTTGTCCGTGAGATCGCTC+AGG - chr4.2:23184262-23184281 MS.gene39538:CDS 55.0%
TGAGGAGCTCAGTGCTCTTC+TGG + chr4.2:23184220-23184239 None:intergenic 55.0%
TTCTTGCAAAGCCGACACGG+CGG + chr4.2:23184318-23184337 None:intergenic 55.0%
! GGATTTGCGAGCGGTTTGCT+TGG + chr4.2:23184066-23184085 None:intergenic 55.0%
!! CGTGTCGGCTTTGCAAGAAG+CGG - chr4.2:23184319-23184338 MS.gene39538:CDS 55.0%
AGCCGCTCGCAAATCTGCTC+CGG - chr4.2:23184124-23184143 MS.gene39538:CDS 60.0%
AGGTTTCTTCACTCCGCCGG+TGG + chr4.2:23184150-23184169 None:intergenic 60.0%
CACGGACAAGACGCTGGAAG+GGG + chr4.2:23184253-23184272 None:intergenic 60.0%
CGACGGTTCCTGGCCTGAAA+CGG + chr4.2:23184175-23184194 None:intergenic 60.0%
CGATCTCACGGACAAGACGC+TGG + chr4.2:23184259-23184278 None:intergenic 60.0%
CTCACGGACAAGACGCTGGA+AGG + chr4.2:23184255-23184274 None:intergenic 60.0%
GACGCTGGAAGGGGAGTTTG+CGG + chr4.2:23184244-23184263 None:intergenic 60.0%
GGAAGGGGAGTTTGCGGATG+AGG + chr4.2:23184238-23184257 None:intergenic 60.0%
GTTCCTGGCCTGAAACGGTG+AGG + chr4.2:23184170-23184189 None:intergenic 60.0%
TTTCCAGAGCTCCGCCGTGT+CGG - chr4.2:23184304-23184323 MS.gene39538:CDS 60.0%
!! ACACGGCGGAGCTCTGGAAA+CGG + chr4.2:23184304-23184323 None:intergenic 60.0%
AAATCTGCTCCGGCCACCGG+CGG - chr4.2:23184134-23184153 MS.gene39538:CDS 65.0%
ACCGCTCGCAAATCCACCGG+TGG - chr4.2:23184071-23184090 MS.gene39538:CDS 65.0%
CACGGATACGCCTAGCGAGC+TGG + chr4.2:23184430-23184449 None:intergenic 65.0%
CAGCTCGCTAGGCGTATCCG+TGG - chr4.2:23184428-23184447 MS.gene39538:CDS 65.0%
CCACCGGTGGCAAAGCTCCA+AGG - chr4.2:23184084-23184103 MS.gene39538:CDS 65.0%
CCTTGGAGCTTTGCCACCGG+TGG + chr4.2:23184087-23184106 None:intergenic 65.0%
CGCAAATCTGCTCCGGCCAC+CGG - chr4.2:23184131-23184150 MS.gene39538:CDS 65.0%
GCGGATCTCACGGAGAGCGA+CGG + chr4.2:23184192-23184211 None:intergenic 65.0%
GCGGCCGAGGCTTATCTCGT+CGG - chr4.2:23184338-23184357 MS.gene39538:CDS 65.0%
GGCCGGAGCAGATTTGCGAG+CGG + chr4.2:23184129-23184148 None:intergenic 65.0%
GGCTTTGCAAGAAGCGGCCG+AGG - chr4.2:23184325-23184344 MS.gene39538:CDS 65.0%
TCACGGAGAGCGACGGTTCC+TGG + chr4.2:23184185-23184204 None:intergenic 65.0%
TTCTTCACTCCGCCGGTGGC+CGG + chr4.2:23184146-23184165 None:intergenic 65.0%
! GCCACCGGTGGATTTGCGAG+CGG + chr4.2:23184075-23184094 None:intergenic 65.0%
AAGCCGACACGGCGGAGCTC+TGG + chr4.2:23184310-23184329 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23184053 23184463 23184053 ID=MS.gene39538
chr4.2 mRNA 23184053 23184463 23184053 ID=MS.gene39538.t1;Parent=MS.gene39538
chr4.2 exon 23184053 23184463 23184053 ID=MS.gene39538.t1.exon1;Parent=MS.gene39538.t1
chr4.2 CDS 23184053 23184463 23184053 ID=cds.MS.gene39538.t1;Parent=MS.gene39538.t1
Gene Sequence

>MS.gene39538

ATGGCACGTACCAAGCAAACCGCTCGCAAATCCACCGGTGGCAAAGCTCCAAGGAAGCAACTCGCCACAAAAGCCGCTCGCAAATCTGCTCCGGCCACCGGCGGAGTGAAGAAACCTCACCGTTTCAGGCCAGGAACCGTCGCTCTCCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTCCTCATCCGCAAACTCCCCTTCCAGCGTCTTGTCCGTGAGATCGCTCAGGATTTCAAGACTGATCTCCGTTTCCAGAGCTCCGCCGTGTCGGCTTTGCAAGAAGCGGCCGAGGCTTATCTCGTCGGTCTCTTTGAGGATACTAACCTCTGCGCCATTCATGCTAAGCGTGTCACTATCATGCCTAAGGATATCCAGCTCGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

Protein sequence

>MS.gene39538.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA