AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97703


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97703.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97703.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene97703.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97703.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97703.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97703.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene97703.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene97703.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene97703.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene97703.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene97703.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene97703.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene97703.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene97703.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene97703.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene97703.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene97703.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene97703.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene97703.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene97703.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene97703.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene97703.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene97703.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene97703.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97703.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97703.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97703.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97703.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97703.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97703.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97703.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene97703.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene97703.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene97703.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene97703.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene97703.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene97703.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97703.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97703.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97703.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGCCGGAGCAGATTTC+CGG 0.243304 5.1:-20404343 None:intergenic
TTGGTGGCTAGCTGCTTTCT+TGG 0.277587 5.1:-20404314 None:intergenic
TCACGAAGTGCAACGGTTCC+TGG 0.291776 5.1:-20404395 None:intergenic
TTCTTGACTCCTCCGGTTGC+CGG 0.293014 5.1:-20404356 None:intergenic
TGAGAAGCTCAGTGCTCTTC+TGG 0.304985 5.1:-20404430 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 5.1:+20404551 MS.gene97703:CDS
GAGCAGAAACAGCGCTGCTT+TGG 0.363298 5.1:-20404520 None:intergenic
AGCAGATTTCCGGGCAGCTT+TGG 0.390197 5.1:-20404333 None:intergenic
CGGAAATCTGCTCCGGCAAC+CGG 0.392471 5.1:+20404344 MS.gene97703:CDS
GGTTGCCGGAGCAGATTTCC+GGG 0.407337 5.1:-20404342 None:intergenic
CTTTCTTGGTGCCTTTCCTC+CGG 0.425692 5.1:-20404300 None:intergenic
TTATCTTGTTGGTTTGTTTG+AGG 0.428809 5.1:+20404562 MS.gene97703:CDS
CGATTTCACGAACAAGTCTC+TGG 0.432729 5.1:-20404469 None:intergenic
AGATTTCCGGGCAGCTTTGG+TGG 0.468887 5.1:-20404330 None:intergenic
AAACAGCGCTGCTTTGGAAT+CGG 0.473836 5.1:-20404514 None:intergenic
AGCTAGCCACCAAAGCTGCC+CGG 0.491500 5.1:+20404324 MS.gene97703:CDS
AGTAACTCTCTTGGCATGAA+TGG 0.491789 5.1:-20404600 None:intergenic
CGAGCGAGTTGAATATCCTT+AGG 0.499677 5.1:-20404629 None:intergenic
GTGTGGCTTCTTGACTCCTC+CGG 0.501667 5.1:-20404363 None:intergenic
AGCTGCCCGGAAATCTGCTC+CGG 0.505877 5.1:+20404337 MS.gene97703:CDS
TCGCAAATCCACCGGAGGAA+AGG 0.532804 5.1:+20404289 MS.gene97703:CDS
TGTTTGCTTTGTACGAGCCA+TGG 0.537743 5.1:-20404264 None:intergenic
AGGCATGATAGTAACTCTCT+TGG 0.540277 5.1:-20404609 None:intergenic
ACTTGTTCGTGAAATCGCTC+AGG 0.551065 5.1:+20404475 MS.gene97703:CDS
GAGAGTTACTATCATGCCTA+AGG 0.552184 5.1:+20404613 MS.gene97703:CDS
CAACTCGCTCGCAGAATCAG+AGG 0.556058 5.1:+20404641 MS.gene97703:CDS
TCTTGGTGCCTTTCCTCCGG+TGG 0.556160 5.1:-20404297 None:intergenic
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 5.1:-20404421 None:intergenic
TCGCAGAATCAGAGGCGAGA+GGG 0.584947 5.1:+20404649 MS.gene97703:CDS
CAAACAGCTCGCAAATCCAC+CGG 0.586123 5.1:+20404281 MS.gene97703:CDS
TCACGAACAAGTCTCTGGAA+TGG 0.590006 5.1:-20404464 None:intergenic
CTCGCAGAATCAGAGGCGAG+AGG 0.599896 5.1:+20404648 MS.gene97703:CDS
AAATCTGCTCCGGCAACCGG+AGG 0.607496 5.1:+20404347 MS.gene97703:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 5.1:+20404377 MS.gene97703:CDS
GCGGATCTCACGAAGTGCAA+CGG 0.633057 5.1:-20404402 None:intergenic
GAAGTGCAACGGTTCCTGGA+CGG 0.655834 5.1:-20404391 None:intergenic
GTTCCTGGACGGAATCTGTG+TGG 0.669194 5.1:-20404380 None:intergenic
ACAGCTCGCAAATCCACCGG+AGG 0.704783 5.1:+20404284 MS.gene97703:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCTTGTTGGTTTGTTTG+AGG + chr5.1:20404562-20404581 MS.gene97703:CDS 30.0%
AGGCATGATAGTAACTCTCT+TGG - chr5.1:20404612-20404631 None:intergenic 40.0%
AGTAACTCTCTTGGCATGAA+TGG - chr5.1:20404603-20404622 None:intergenic 40.0%
GAGAGTTACTATCATGCCTA+AGG + chr5.1:20404613-20404632 MS.gene97703:CDS 40.0%
ACTTGTTCGTGAAATCGCTC+AGG + chr5.1:20404475-20404494 MS.gene97703:CDS 45.0%
CGATTTCACGAACAAGTCTC+TGG - chr5.1:20404472-20404491 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG + chr5.1:20404551-20404570 MS.gene97703:CDS 45.0%
! CGAGCGAGTTGAATATCCTT+AGG - chr5.1:20404632-20404651 None:intergenic 45.0%
! TCACGAACAAGTCTCTGGAA+TGG - chr5.1:20404467-20404486 None:intergenic 45.0%
!! AAACAGCGCTGCTTTGGAAT+CGG - chr5.1:20404517-20404536 None:intergenic 45.0%
CAAACAGCTCGCAAATCCAC+CGG + chr5.1:20404281-20404300 MS.gene97703:CDS 50.0%
CAGTGCTCTTCTGGTACTTG+CGG - chr5.1:20404424-20404443 None:intergenic 50.0%
TGAGAAGCTCAGTGCTCTTC+TGG - chr5.1:20404433-20404452 None:intergenic 50.0%
! TTGGTGGCTAGCTGCTTTCT+TGG - chr5.1:20404317-20404336 None:intergenic 50.0%
!! CTTTCTTGGTGCCTTTCCTC+CGG - chr5.1:20404303-20404322 None:intergenic 50.0%
AAGCCACACAGATTCCGTCC+AGG + chr5.1:20404377-20404396 MS.gene97703:CDS 55.0%
AGCAGATTTCCGGGCAGCTT+TGG - chr5.1:20404336-20404355 None:intergenic 55.0%
CAACTCGCTCGCAGAATCAG+AGG + chr5.1:20404641-20404660 MS.gene97703:CDS 55.0%
GAAGTGCAACGGTTCCTGGA+CGG - chr5.1:20404394-20404413 None:intergenic 55.0%
GAGCAGAAACAGCGCTGCTT+TGG - chr5.1:20404523-20404542 None:intergenic 55.0%
GCGGATCTCACGAAGTGCAA+CGG - chr5.1:20404405-20404424 None:intergenic 55.0%
GTGTGGCTTCTTGACTCCTC+CGG - chr5.1:20404366-20404385 None:intergenic 55.0%
GTTCCTGGACGGAATCTGTG+TGG - chr5.1:20404383-20404402 None:intergenic 55.0%
TCACGAAGTGCAACGGTTCC+TGG - chr5.1:20404398-20404417 None:intergenic 55.0%
TCGCAAATCCACCGGAGGAA+AGG + chr5.1:20404289-20404308 MS.gene97703:CDS 55.0%
TCGCAGAATCAGAGGCGAGA+GGG + chr5.1:20404649-20404668 MS.gene97703:CDS 55.0%
TTCTTGACTCCTCCGGTTGC+CGG - chr5.1:20404359-20404378 None:intergenic 55.0%
! AGATTTCCGGGCAGCTTTGG+TGG - chr5.1:20404333-20404352 None:intergenic 55.0%
AAATCTGCTCCGGCAACCGG+AGG + chr5.1:20404347-20404366 MS.gene97703:CDS 60.0%
ACAGCTCGCAAATCCACCGG+AGG + chr5.1:20404284-20404303 MS.gene97703:CDS 60.0%
AGCTAGCCACCAAAGCTGCC+CGG + chr5.1:20404324-20404343 MS.gene97703:CDS 60.0%
AGCTGCCCGGAAATCTGCTC+CGG + chr5.1:20404337-20404356 MS.gene97703:CDS 60.0%
CGGAAATCTGCTCCGGCAAC+CGG + chr5.1:20404344-20404363 MS.gene97703:CDS 60.0%
CGGTTGCCGGAGCAGATTTC+CGG - chr5.1:20404346-20404365 None:intergenic 60.0%
CTCGCAGAATCAGAGGCGAG+AGG + chr5.1:20404648-20404667 MS.gene97703:CDS 60.0%
GGTTGCCGGAGCAGATTTCC+GGG - chr5.1:20404345-20404364 None:intergenic 60.0%
!! TCTTGGTGCCTTTCCTCCGG+TGG - chr5.1:20404300-20404319 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20404266 20404676 20404266 ID=MS.gene97703
chr5.1 mRNA 20404266 20404676 20404266 ID=MS.gene97703.t1;Parent=MS.gene97703
chr5.1 exon 20404266 20404676 20404266 ID=MS.gene97703.t1.exon1;Parent=MS.gene97703.t1
chr5.1 CDS 20404266 20404676 20404266 ID=cds.MS.gene97703.t1;Parent=MS.gene97703.t1
Gene Sequence

>MS.gene97703

ATGGCTCGTACAAAGCAAACAGCTCGCAAATCCACCGGAGGAAAGGCACCAAGAAAGCAGCTAGCCACCAAAGCTGCCCGGAAATCTGCTCCGGCAACCGGAGGAGTCAAGAAGCCACACAGATTCCGTCCAGGAACCGTTGCACTTCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGAAAGCTTCCATTCCAGAGACTTGTTCGTGAAATCGCTCAGGATTTCAAAACAGATCTCCGATTCCAAAGCAGCGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAGGATACTAATCTCTGTGCCATTCATGCCAAGAGAGTTACTATCATGCCTAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MS.gene97703.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA