AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97701


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97701.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene97701.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene97701.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97701.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97701.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene97701.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene97701.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene97701.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene97701.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene97701.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene97701.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene97701.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene97701.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene97701.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene97701.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene97701.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene97701.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene97701.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene97701.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene97701.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene97701.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene97701.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene97701.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene97701.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97701.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97701.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97701.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97701.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97701.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene97701.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene97701.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene97701.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene97701.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene97701.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene97701.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene97701.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene97701.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97701.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97701.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene97701.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTGCCGGAGCAGATTTC+CGG 0.243304 5.1:+20421288 None:intergenic
TTCTTGACTCCTCCGGTTGC+CGG 0.293014 5.1:+20421275 None:intergenic
TGAGAAGTTCAGTGCTCTTC+TGG 0.315268 5.1:+20421201 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 5.1:-20421080 MS.gene97701:CDS
TCACGAAGTGCAACAGTTCC+AGG 0.377831 5.1:+20421236 None:intergenic
AGCAGATTTCCGGGCAGCTT+TGG 0.390197 5.1:+20421298 None:intergenic
CGGAAATCTGCTCCGGCAAC+CGG 0.392471 5.1:-20421287 MS.gene97701:CDS
CTTCCTTGGTGCCTTTCCTC+CGG 0.399049 5.1:+20421331 None:intergenic
GGTTGCCGGAGCAGATTTCC+GGG 0.407337 5.1:+20421289 None:intergenic
TTGGTGGCTAGCTGCTTCCT+TGG 0.412728 5.1:+20421317 None:intergenic
GAGCAGAAACAGCACTGCTT+TGG 0.422995 5.1:+20421111 None:intergenic
TTATCTTGTTGGTTTGTTTG+AGG 0.428809 5.1:-20421069 MS.gene97701:CDS
CGATTTCACGAACAAGTCTC+TGG 0.432729 5.1:+20421162 None:intergenic
AGATTTCCGGGCAGCTTTGG+TGG 0.468887 5.1:+20421301 None:intergenic
AAACAGCACTGCTTTGGAAT+CGG 0.472990 5.1:+20421117 None:intergenic
AGCTAGCCACCAAAGCTGCC+CGG 0.491500 5.1:-20421307 MS.gene97701:CDS
CGAGCGAGTTGAATATCCTT+AGG 0.499677 5.1:+20421002 None:intergenic
GTGTGGCTTCTTGACTCCTC+CGG 0.501667 5.1:+20421268 None:intergenic
AGCTGCCCGGAAATCTGCTC+CGG 0.505877 5.1:-20421294 MS.gene97701:CDS
TCGCAAATCCACCGGAGGAA+AGG 0.508505 5.1:-20421342 MS.gene97701:CDS
CCTTGGTGCCTTTCCTCCGG+TGG 0.517647 5.1:+20421334 None:intergenic
AGAGCACTGAACTTCTCATA+AGG 0.521842 5.1:-20421196 MS.gene97701:CDS
AGTAACTCTCTTAGCATGAA+TGG 0.521941 5.1:+20421031 None:intergenic
GAGAGTTACTATCATGCCTA+AGG 0.550012 5.1:-20421018 MS.gene97701:CDS
ACTTGTTCGTGAAATCGCTC+AGG 0.551065 5.1:-20421156 MS.gene97701:CDS
CAACTCGCTCGCAGAATCAG+AGG 0.556058 5.1:-20420990 MS.gene97701:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.559165 5.1:+20421210 None:intergenic
TCACGAACAAGTCTCTGGAA+TGG 0.590006 5.1:+20421167 None:intergenic
AAATCTGCTCCGGCAACCGG+AGG 0.607496 5.1:-20421284 MS.gene97701:CDS
AAGCCACACAGATTCCGTCC+TGG 0.622196 5.1:-20421254 MS.gene97701:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.629023 5.1:-20421350 MS.gene97701:CDS
TCCTCCGGTGGATTTGCGAG+CGG 0.642208 5.1:+20421346 None:intergenic
CCACCGGAGGAAAGGCACCA+AGG 0.647774 5.1:-20421334 MS.gene97701:CDS
ACCGCTCGCAAATCCACCGG+AGG 0.676829 5.1:-20421347 MS.gene97701:CDS
GAAGTGCAACAGTTCCAGGA+CGG 0.690156 5.1:+20421240 None:intergenic
GTTCCAGGACGGAATCTGTG+TGG 0.699556 5.1:+20421251 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCTTGTTGGTTTGTTTG+AGG - chr5.1:20421273-20421292 MS.gene97701:CDS 30.0%
AGTAACTCTCTTAGCATGAA+TGG + chr5.1:20421314-20421333 None:intergenic 35.0%
GAGAGTTACTATCATGCCTA+AGG - chr5.1:20421324-20421343 MS.gene97701:CDS 40.0%
!! AAACAGCACTGCTTTGGAAT+CGG + chr5.1:20421228-20421247 None:intergenic 40.0%
!! AGAGCACTGAACTTCTCATA+AGG - chr5.1:20421146-20421165 MS.gene97701:CDS 40.0%
ACTTGTTCGTGAAATCGCTC+AGG - chr5.1:20421186-20421205 MS.gene97701:CDS 45.0%
CGATTTCACGAACAAGTCTC+TGG + chr5.1:20421183-20421202 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG - chr5.1:20421262-20421281 MS.gene97701:CDS 45.0%
TGAGAAGTTCAGTGCTCTTC+TGG + chr5.1:20421144-20421163 None:intergenic 45.0%
! CGAGCGAGTTGAATATCCTT+AGG + chr5.1:20421343-20421362 None:intergenic 45.0%
! TCACGAACAAGTCTCTGGAA+TGG + chr5.1:20421178-20421197 None:intergenic 45.0%
CAGTGCTCTTCTGGTACTTG+CGG + chr5.1:20421135-20421154 None:intergenic 50.0%
GAAGTGCAACAGTTCCAGGA+CGG + chr5.1:20421105-20421124 None:intergenic 50.0%
GAGCAGAAACAGCACTGCTT+TGG + chr5.1:20421234-20421253 None:intergenic 50.0%
TCACGAAGTGCAACAGTTCC+AGG + chr5.1:20421109-20421128 None:intergenic 50.0%
AAGCCACACAGATTCCGTCC+TGG - chr5.1:20421088-20421107 MS.gene97701:CDS 55.0%
AGCAGATTTCCGGGCAGCTT+TGG + chr5.1:20421047-20421066 None:intergenic 55.0%
CAAACCGCTCGCAAATCCAC+CGG - chr5.1:20420992-20421011 MS.gene97701:CDS 55.0%
CAACTCGCTCGCAGAATCAG+AGG - chr5.1:20421352-20421371 MS.gene97701:CDS 55.0%
GTGTGGCTTCTTGACTCCTC+CGG + chr5.1:20421077-20421096 None:intergenic 55.0%
GTTCCAGGACGGAATCTGTG+TGG + chr5.1:20421094-20421113 None:intergenic 55.0%
TCGCAAATCCACCGGAGGAA+AGG - chr5.1:20421000-20421019 MS.gene97701:CDS 55.0%
TTCTTGACTCCTCCGGTTGC+CGG + chr5.1:20421070-20421089 None:intergenic 55.0%
! AGATTTCCGGGCAGCTTTGG+TGG + chr5.1:20421044-20421063 None:intergenic 55.0%
! TTGGTGGCTAGCTGCTTCCT+TGG + chr5.1:20421028-20421047 None:intergenic 55.0%
!! CTTCCTTGGTGCCTTTCCTC+CGG + chr5.1:20421014-20421033 None:intergenic 55.0%
AAATCTGCTCCGGCAACCGG+AGG - chr5.1:20421058-20421077 MS.gene97701:CDS 60.0%
AGCTAGCCACCAAAGCTGCC+CGG - chr5.1:20421035-20421054 MS.gene97701:CDS 60.0%
AGCTGCCCGGAAATCTGCTC+CGG - chr5.1:20421048-20421067 MS.gene97701:CDS 60.0%
CGGAAATCTGCTCCGGCAAC+CGG - chr5.1:20421055-20421074 MS.gene97701:CDS 60.0%
CGGTTGCCGGAGCAGATTTC+CGG + chr5.1:20421057-20421076 None:intergenic 60.0%
GGTTGCCGGAGCAGATTTCC+GGG + chr5.1:20421056-20421075 None:intergenic 60.0%
! TCCTCCGGTGGATTTGCGAG+CGG + chr5.1:20420999-20421018 None:intergenic 60.0%
ACCGCTCGCAAATCCACCGG+AGG - chr5.1:20420995-20421014 MS.gene97701:CDS 65.0%
!! CCACCGGAGGAAAGGCACCA+AGG - chr5.1:20421008-20421027 MS.gene97701:CDS 65.0%
!! CCTTGGTGCCTTTCCTCCGG+TGG + chr5.1:20421011-20421030 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20420977 20421387 20420977 ID=MS.gene97701
chr5.1 mRNA 20420977 20421387 20420977 ID=MS.gene97701.t1;Parent=MS.gene97701
chr5.1 exon 20420977 20421387 20420977 ID=MS.gene97701.t1.exon1;Parent=MS.gene97701.t1
chr5.1 CDS 20420977 20421387 20420977 ID=cds.MS.gene97701.t1;Parent=MS.gene97701.t1
Gene Sequence

>MS.gene97701

ATGGCTCGTACAAAACAAACCGCTCGCAAATCCACCGGAGGAAAGGCACCAAGGAAGCAGCTAGCCACCAAAGCTGCCCGGAAATCTGCTCCGGCAACCGGAGGAGTCAAGAAGCCACACAGATTCCGTCCTGGAACTGTTGCACTTCGTGAGATCCGCAAGTACCAGAAGAGCACTGAACTTCTCATAAGGAAGCTTCCATTCCAGAGACTTGTTCGTGAAATCGCTCAGGATTTCAAAACAGATCTCCGATTCCAAAGCAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAGGATACTAATCTCTGTGCCATTCATGCTAAGAGAGTTACTATCATGCCTAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGAGCTTAG

Protein sequence

>MS.gene97701.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA