AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65125


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65125.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene65125.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene65125.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene65125.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene65125.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene65125.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene65125.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene65125.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene65125.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene65125.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene65125.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene65125.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene65125.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene65125.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene65125.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene65125.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene65125.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene65125.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene65125.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene65125.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene65125.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene65125.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene65125.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene65125.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65125.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene65125.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene65125.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene65125.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene65125.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene65125.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene65125.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene65125.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene65125.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene65125.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene65125.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene65125.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene65125.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene65125.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene65125.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene65125.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 35 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGATTTCCGTGCGGCTT+TGG 0.327301 7.1:+42449885 None:intergenic
TTCACGAAGCGCGACGGTTC+CGG 0.341493 7.1:+42449822 None:intergenic
TCACGAAGCGCGACGGTTCC+GGG 0.353522 7.1:+42449823 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 7.1:-42449667 MS.gene65125:CDS
GGATTTGCGTGCTGTTTGTT+TGG 0.431176 7.1:+42449942 None:intergenic
TGATTTCCGTGCGGCTTTGG+TGG 0.441149 7.1:+42449888 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 7.1:+42449862 None:intergenic
CTTGCAAGTTGAATATCCTT+AGG 0.450286 7.1:+42449589 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.469854 7.1:-42449874 MS.gene65125:CDS
CAGTGCTCTTCTGATACTTA+CGG 0.470671 7.1:+42449797 None:intergenic
TGGTGGCGAGTTGTTTGCGT+GGG 0.485319 7.1:+42449905 None:intergenic
CAAGGAGAATTCGTGGTGAA+AGG 0.485929 7.1:-42449570 MS.gene65125:CDS
AGCCGCACGGAAATCAGCTC+CGG 0.497667 7.1:-42449881 MS.gene65125:CDS
CGACGGTTCCGGGACGGAAA+CGG 0.513929 7.1:+42449833 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 7.1:-42449605 MS.gene65125:CDS
AAGGAGAATTCGTGGTGAAA+GGG 0.519150 7.1:-42449569 MS.gene65125:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.526132 7.1:+42449855 None:intergenic
CAACTTGCAAGGAGAATTCG+TGG 0.532409 7.1:-42449577 MS.gene65125:CDS
AGAGCACTGAGCTTCTGATA+AGG 0.532730 7.1:-42449783 MS.gene65125:CDS
AAGCCACACCGTTTCCGTCC+CGG 0.544193 7.1:-42449841 MS.gene65125:CDS
CAAACAGCACGCAAATCCAC+CGG 0.549355 7.1:-42449937 MS.gene65125:CDS
TCTTGTTCGTGAAATCGCTC+AGG 0.549771 7.1:-42449743 MS.gene65125:CDS
CTAAGGATATTCAACTTGCA+AGG 0.586821 7.1:-42449588 MS.gene65125:CDS
TTGGTGGCGAGTTGTTTGCG+TGG 0.598004 7.1:+42449904 None:intergenic
TCACGAACAAGACGTTGGAA+TGG 0.598115 7.1:+42449754 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.606043 7.1:-42449871 MS.gene65125:CDS
ACTGCTCTGAAATCTGAGAT+CGG 0.610183 7.1:+42449711 None:intergenic
CGATTTCACGAACAAGACGT+TGG 0.613448 7.1:+42449749 None:intergenic
ACGGATTTCACGAAGCGCGA+CGG 0.624291 7.1:+42449816 None:intergenic
AACTCGCCACCAAAGCCGCA+CGG 0.635817 7.1:-42449894 MS.gene65125:CDS
GAAGCGCGACGGTTCCGGGA+CGG 0.640203 7.1:+42449827 None:intergenic
GTTCCGGGACGGAAACGGTG+TGG 0.652674 7.1:+42449838 None:intergenic
ACAGCACGCAAATCCACCGG+CGG 0.691035 7.1:-42449934 MS.gene65125:CDS
GGTGGCGAGTTGTTTGCGTG+GGG 0.692652 7.1:+42449906 None:intergenic
CGCCGGAGCTGATTTCCGTG+CGG 0.701307 7.1:+42449879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTAAGGATATTCAACTTGCA+AGG - chr7.1:42449928-42449947 MS.gene65125:CDS 35.0%
GAGAGTTACTATTATGCCTA+AGG - chr7.1:42449911-42449930 MS.gene65125:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG + chr7.1:42449930-42449949 None:intergenic 35.0%
AAGGAGAATTCGTGGTGAAA+GGG - chr7.1:42449947-42449966 MS.gene65125:CDS 40.0%
CAGTGCTCTTCTGATACTTA+CGG + chr7.1:42449722-42449741 None:intergenic 40.0%
!! ACTGCTCTGAAATCTGAGAT+CGG + chr7.1:42449808-42449827 None:intergenic 40.0%
CAACTTGCAAGGAGAATTCG+TGG - chr7.1:42449939-42449958 MS.gene65125:CDS 45.0%
CAAGGAGAATTCGTGGTGAA+AGG - chr7.1:42449946-42449965 MS.gene65125:CDS 45.0%
CGATTTCACGAACAAGACGT+TGG + chr7.1:42449770-42449789 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG - chr7.1:42449849-42449868 MS.gene65125:CDS 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr7.1:42449765-42449784 None:intergenic 45.0%
TCTTGTTCGTGAAATCGCTC+AGG - chr7.1:42449773-42449792 MS.gene65125:CDS 45.0%
!! AGAGCACTGAGCTTCTGATA+AGG - chr7.1:42449733-42449752 MS.gene65125:CDS 45.0%
!! GGATTTGCGTGCTGTTTGTT+TGG + chr7.1:42449577-42449596 None:intergenic 45.0%
CAAACAGCACGCAAATCCAC+CGG - chr7.1:42449579-42449598 MS.gene65125:CDS 50.0%
ACGGATTTCACGAAGCGCGA+CGG + chr7.1:42449703-42449722 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr7.1:42449664-42449683 None:intergenic 55.0%
! AGCTGATTTCCGTGCGGCTT+TGG + chr7.1:42449634-42449653 None:intergenic 55.0%
! TGATTTCCGTGCGGCTTTGG+TGG + chr7.1:42449631-42449650 None:intergenic 55.0%
! TGGTGGCGAGTTGTTTGCGT+GGG + chr7.1:42449614-42449633 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTGCG+TGG + chr7.1:42449615-42449634 None:intergenic 55.0%
AACTCGCCACCAAAGCCGCA+CGG - chr7.1:42449622-42449641 MS.gene65125:CDS 60.0%
AAGCCACACCGTTTCCGTCC+CGG - chr7.1:42449675-42449694 MS.gene65125:CDS 60.0%
ACAGCACGCAAATCCACCGG+CGG - chr7.1:42449582-42449601 MS.gene65125:CDS 60.0%
AGCCGCACGGAAATCAGCTC+CGG - chr7.1:42449635-42449654 MS.gene65125:CDS 60.0%
GGTGGCGAGTTGTTTGCGTG+GGG + chr7.1:42449613-42449632 None:intergenic 60.0%
TTCACGAAGCGCGACGGTTC+CGG + chr7.1:42449697-42449716 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr7.1:42449657-42449676 None:intergenic 60.0%
! TTTGCGTGGGGCTTTTCCGC+CGG + chr7.1:42449601-42449620 None:intergenic 60.0%
AAATCAGCTCCGGCGACCGG+AGG - chr7.1:42449645-42449664 MS.gene65125:CDS 65.0%
CGACGGTTCCGGGACGGAAA+CGG + chr7.1:42449686-42449705 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+CGG - chr7.1:42449642-42449661 MS.gene65125:CDS 65.0%
GTTCCGGGACGGAAACGGTG+TGG + chr7.1:42449681-42449700 None:intergenic 65.0%
TCACGAAGCGCGACGGTTCC+GGG + chr7.1:42449696-42449715 None:intergenic 65.0%
! CGCCGGAGCTGATTTCCGTG+CGG + chr7.1:42449640-42449659 None:intergenic 65.0%
GAAGCGCGACGGTTCCGGGA+CGG + chr7.1:42449692-42449711 None:intergenic 70.0%
! GCGTGGGGCTTTTCCGCCGG+TGG + chr7.1:42449598-42449617 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 42449564 42449974 42449564 ID=MS.gene65125
chr7.1 mRNA 42449564 42449974 42449564 ID=MS.gene65125.t1;Parent=MS.gene65125
chr7.1 exon 42449564 42449974 42449564 ID=MS.gene65125.t1.exon1;Parent=MS.gene65125.t1
chr7.1 CDS 42449564 42449974 42449564 ID=cds.MS.gene65125.t1;Parent=MS.gene65125.t1
Gene Sequence

>MS.gene65125

ATGGCACGTACCAAACAAACAGCACGCAAATCCACCGGCGGAAAAGCCCCACGCAAACAACTCGCCACCAAAGCCGCACGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACCGTTTCCGTCCCGGAACCGTCGCGCTTCGTGAAATCCGTAAGTATCAGAAGAGCACTGAGCTTCTGATAAGGAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAGGATTTCAAAACCGATCTCAGATTTCAGAGCAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACAAATCTTTGCGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGGAGAATTCGTGGTGAAAGGGCTTAG

Protein sequence

>MS.gene65125.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA