AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47573


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47573.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene47573.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene47573.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene47573.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene47573.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene47573.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene47573.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene47573.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene47573.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene47573.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene47573.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene47573.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene47573.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene47573.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene47573.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene47573.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene47573.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene47573.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene47573.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene47573.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene47573.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene47573.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene47573.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene47573.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47573.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene47573.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene47573.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene47573.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene47573.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene47573.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene47573.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene47573.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene47573.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene47573.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene47573.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene47573.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene47573.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene47573.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene47573.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene47573.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAACCTCACCGTTTC+AGG 0.224781 4.3:-25315894 MS.gene47573:CDS
TCACGGAGAGCGACGGTTCC+TGG 0.319730 4.3:+25315871 None:intergenic
TGAGGAGCTCAGTGCTCTTC+TGG 0.339681 4.3:+25315836 None:intergenic
AAGCCGACACGGCGGAGCTC+TGG 0.377718 4.3:+25315746 None:intergenic
TTCTTCACTCCGCCGGTGGC+CGG 0.393192 4.3:+25315910 None:intergenic
TTTGTGGCGAGTTGCTTCCT+TGG 0.400731 4.3:+25315952 None:intergenic
TTATCTCGTCGGTCTCTTTG+AGG 0.447224 4.3:-25315704 MS.gene47573:CDS
GGAAGGGGAGTTTGCGTATG+AGG 0.450631 4.3:+25315818 None:intergenic
CTTCCTTGGAGCTTTGCCAC+CGG 0.456245 4.3:+25315966 None:intergenic
CGCAAATCTGCTCCGGCCAC+CGG 0.457456 4.3:-25315922 MS.gene47573:CDS
AGTGACACGCTTAGCATGAA+TGG 0.474744 4.3:+25315666 None:intergenic
CCTTGGAGCTTTGCCACCGG+TGG 0.488901 4.3:+25315969 None:intergenic
CGCTTCTTGCAAAGCCGACA+CGG 0.492590 4.3:+25315735 None:intergenic
CGACGGTTCCTGGCCTGAAA+CGG 0.503357 4.3:+25315881 None:intergenic
GGCTTTGCAAGAAGCGGCCG+AGG 0.504571 4.3:-25315728 MS.gene47573:CDS
GGATTTGCGAGCGGTTTGCT+TGG 0.506530 4.3:+25315990 None:intergenic
CTAGCGAGCTGGATATCCTT+AGG 0.510191 4.3:+25315637 None:intergenic
AAACCTCACCGTTTCAGGCC+AGG 0.512079 4.3:-25315889 MS.gene47573:CDS
CACGGATACGCCTAGCGAGC+TGG 0.513784 4.3:+25315626 None:intergenic
TTTCCAGAGCTCCGCCGTGT+CGG 0.519570 4.3:-25315749 MS.gene47573:CDS
ACACGGCGGAGCTCTGGAAA+CGG 0.525377 4.3:+25315752 None:intergenic
AGCCGCTCGCAAATCTGCTC+CGG 0.526992 4.3:-25315929 MS.gene47573:CDS
CACGGACAAGACGCTGGAAG+GGG 0.529109 4.3:+25315803 None:intergenic
CTCACGGACAAGACGCTGGA+AGG 0.539901 4.3:+25315801 None:intergenic
GTGAGGTTTCTTCACTCCGC+CGG 0.553823 4.3:+25315903 None:intergenic
CTAAGGATATCCAGCTCGCT+AGG 0.554905 4.3:-25315636 MS.gene47573:CDS
GCGTGTCACTATCATGCCTA+AGG 0.557496 4.3:-25315653 MS.gene47573:CDS
TGAAATCCTGAGCGATCTCA+CGG 0.570370 4.3:+25315785 None:intergenic
TCTGGTACTTGCGGATCTCA+CGG 0.571857 4.3:+25315854 None:intergenic
CCACCGGTGGCAAAGCTCCA+AGG 0.572773 4.3:-25315969 MS.gene47573:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.579006 4.3:+25315845 None:intergenic
TCACGGACAAGACGCTGGAA+GGG 0.583031 4.3:+25315802 None:intergenic
AAATCTGCTCCGGCCACCGG+CGG 0.584127 4.3:-25315919 MS.gene47573:CDS
CGATCTCACGGACAAGACGC+TGG 0.585935 4.3:+25315797 None:intergenic
AGGTTTCTTCACTCCGCCGG+TGG 0.590490 4.3:+25315906 None:intergenic
TCTTGTCCGTGAGATCGCTC+AGG 0.603921 4.3:-25315791 MS.gene47573:CDS
GCGGCCGAGGCTTATCTCGT+CGG 0.608232 4.3:-25315715 MS.gene47573:CDS
CAAACCGCTCGCAAATCCAC+CGG 0.613586 4.3:-25315985 MS.gene47573:CDS
ACCGCTCGCAAATCCACCGG+TGG 0.632751 4.3:-25315982 MS.gene47573:CDS
AAGATCAAGCACGCTCGCCA+CGG 0.639365 4.3:+25315608 None:intergenic
GGCCGGAGCAGATTTGCGAG+CGG 0.640066 4.3:+25315927 None:intergenic
GCCACCGGTGGATTTGCGAG+CGG 0.642552 4.3:+25315981 None:intergenic
CGTGTCGGCTTTGCAAGAAG+CGG 0.647453 4.3:-25315734 MS.gene47573:CDS
GAGACCGACGAGATAAGCCT+CGG 0.653052 4.3:+25315711 None:intergenic
GCGGATCTCACGGAGAGCGA+CGG 0.662183 4.3:+25315864 None:intergenic
GTTCCTGGCCTGAAACGGTG+AGG 0.663103 4.3:+25315886 None:intergenic
CAGCTCGCTAGGCGTATCCG+TGG 0.667707 4.3:-25315625 MS.gene47573:CDS
GCTTAGCATGAATGGCGCAG+AGG 0.694649 4.3:+25315674 None:intergenic
TTCTTGCAAAGCCGACACGG+CGG 0.736453 4.3:+25315738 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTGACACGCTTAGCATGAA+TGG + chr4.3:25315949-25315968 None:intergenic 45.0%
TGAAATCCTGAGCGATCTCA+CGG + chr4.3:25315830-25315849 None:intergenic 45.0%
TGAAGAAACCTCACCGTTTC+AGG - chr4.3:25315718-25315737 MS.gene47573:CDS 45.0%
TTATCTCGTCGGTCTCTTTG+AGG - chr4.3:25315908-25315927 MS.gene47573:CDS 45.0%
CAGTGCTCTTCTGGTACTTG+CGG + chr4.3:25315770-25315789 None:intergenic 50.0%
CTAAGGATATCCAGCTCGCT+AGG - chr4.3:25315976-25315995 MS.gene47573:CDS 50.0%
CTAGCGAGCTGGATATCCTT+AGG + chr4.3:25315978-25315997 None:intergenic 50.0%
TCTGGTACTTGCGGATCTCA+CGG + chr4.3:25315761-25315780 None:intergenic 50.0%
TTTGTGGCGAGTTGCTTCCT+TGG + chr4.3:25315663-25315682 None:intergenic 50.0%
! AGATTTGCGAGCGGCTTTTG+TGG + chr4.3:25315679-25315698 None:intergenic 50.0%
! GCGTGTCACTATCATGCCTA+AGG - chr4.3:25315959-25315978 MS.gene47573:CDS 50.0%
AAACCTCACCGTTTCAGGCC+AGG - chr4.3:25315723-25315742 MS.gene47573:CDS 55.0%
CAAACCGCTCGCAAATCCAC+CGG - chr4.3:25315627-25315646 MS.gene47573:CDS 55.0%
CGCTTCTTGCAAAGCCGACA+CGG + chr4.3:25315880-25315899 None:intergenic 55.0%
CTTCCTTGGAGCTTTGCCAC+CGG + chr4.3:25315649-25315668 None:intergenic 55.0%
GAGACCGACGAGATAAGCCT+CGG + chr4.3:25315904-25315923 None:intergenic 55.0%
GCTTAGCATGAATGGCGCAG+AGG + chr4.3:25315941-25315960 None:intergenic 55.0%
GGAAGGGGAGTTTGCGTATG+AGG + chr4.3:25315797-25315816 None:intergenic 55.0%
GTGAGGTTTCTTCACTCCGC+CGG + chr4.3:25315712-25315731 None:intergenic 55.0%
TCACGGACAAGACGCTGGAA+GGG + chr4.3:25315813-25315832 None:intergenic 55.0%
TCTTGTCCGTGAGATCGCTC+AGG - chr4.3:25315821-25315840 MS.gene47573:CDS 55.0%
TGAGGAGCTCAGTGCTCTTC+TGG + chr4.3:25315779-25315798 None:intergenic 55.0%
TTCTTGCAAAGCCGACACGG+CGG + chr4.3:25315877-25315896 None:intergenic 55.0%
! GGATTTGCGAGCGGTTTGCT+TGG + chr4.3:25315625-25315644 None:intergenic 55.0%
!! CGTGTCGGCTTTGCAAGAAG+CGG - chr4.3:25315878-25315897 MS.gene47573:CDS 55.0%
AGCCGCTCGCAAATCTGCTC+CGG - chr4.3:25315683-25315702 MS.gene47573:CDS 60.0%
AGGTTTCTTCACTCCGCCGG+TGG + chr4.3:25315709-25315728 None:intergenic 60.0%
CACGGACAAGACGCTGGAAG+GGG + chr4.3:25315812-25315831 None:intergenic 60.0%
CGACGGTTCCTGGCCTGAAA+CGG + chr4.3:25315734-25315753 None:intergenic 60.0%
CGATCTCACGGACAAGACGC+TGG + chr4.3:25315818-25315837 None:intergenic 60.0%
CTCACGGACAAGACGCTGGA+AGG + chr4.3:25315814-25315833 None:intergenic 60.0%
GTTCCTGGCCTGAAACGGTG+AGG + chr4.3:25315729-25315748 None:intergenic 60.0%
TTTCCAGAGCTCCGCCGTGT+CGG - chr4.3:25315863-25315882 MS.gene47573:CDS 60.0%
!! ACACGGCGGAGCTCTGGAAA+CGG + chr4.3:25315863-25315882 None:intergenic 60.0%
AAATCTGCTCCGGCCACCGG+CGG - chr4.3:25315693-25315712 MS.gene47573:CDS 65.0%
ACCGCTCGCAAATCCACCGG+TGG - chr4.3:25315630-25315649 MS.gene47573:CDS 65.0%
CACGGATACGCCTAGCGAGC+TGG + chr4.3:25315989-25316008 None:intergenic 65.0%
CAGCTCGCTAGGCGTATCCG+TGG - chr4.3:25315987-25316006 MS.gene47573:CDS 65.0%
CCACCGGTGGCAAAGCTCCA+AGG - chr4.3:25315643-25315662 MS.gene47573:CDS 65.0%
CCTTGGAGCTTTGCCACCGG+TGG + chr4.3:25315646-25315665 None:intergenic 65.0%
CGCAAATCTGCTCCGGCCAC+CGG - chr4.3:25315690-25315709 MS.gene47573:CDS 65.0%
GCGGATCTCACGGAGAGCGA+CGG + chr4.3:25315751-25315770 None:intergenic 65.0%
GCGGCCGAGGCTTATCTCGT+CGG - chr4.3:25315897-25315916 MS.gene47573:CDS 65.0%
GGCCGGAGCAGATTTGCGAG+CGG + chr4.3:25315688-25315707 None:intergenic 65.0%
GGCTTTGCAAGAAGCGGCCG+AGG - chr4.3:25315884-25315903 MS.gene47573:CDS 65.0%
TCACGGAGAGCGACGGTTCC+TGG + chr4.3:25315744-25315763 None:intergenic 65.0%
TTCTTCACTCCGCCGGTGGC+CGG + chr4.3:25315705-25315724 None:intergenic 65.0%
! GCCACCGGTGGATTTGCGAG+CGG + chr4.3:25315634-25315653 None:intergenic 65.0%
AAGCCGACACGGCGGAGCTC+TGG + chr4.3:25315869-25315888 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 25315612 25316022 25315612 ID=MS.gene47573
chr4.3 mRNA 25315612 25316022 25315612 ID=MS.gene47573.t1;Parent=MS.gene47573
chr4.3 exon 25315612 25316022 25315612 ID=MS.gene47573.t1.exon1;Parent=MS.gene47573.t1
chr4.3 CDS 25315612 25316022 25315612 ID=cds.MS.gene47573.t1;Parent=MS.gene47573.t1
Gene Sequence

>MS.gene47573

ATGGCACGTACCAAGCAAACCGCTCGCAAATCCACCGGTGGCAAAGCTCCAAGGAAGCAACTCGCCACAAAAGCCGCTCGCAAATCTGCTCCGGCCACCGGCGGAGTGAAGAAACCTCACCGTTTCAGGCCAGGAACCGTCGCTCTCCGTGAGATCCGCAAGTACCAGAAGAGCACTGAGCTCCTCATACGCAAACTCCCCTTCCAGCGTCTTGTCCGTGAGATCGCTCAGGATTTCAAGACTGATCTCCGTTTCCAGAGCTCCGCCGTGTCGGCTTTGCAAGAAGCGGCCGAGGCTTATCTCGTCGGTCTCTTTGAGGATACTAACCTCTGCGCCATTCATGCTAAGCGTGTCACTATCATGCCTAAGGATATCCAGCTCGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

Protein sequence

>MS.gene47573.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA