Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027907.t1 | XP_004512510.2 | 100 | 136 | 0 | 0 | 1 | 136 | 104 | 239 | 5.80E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027907.t1 | P68429 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 1.4e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027907.t1 | A0A1S3TJX1 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.2e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027907.t1 | MTR_7g059070 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_5g029820 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_5g029770 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_4g065990 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_4g088150 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_2g035230 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_8g092720 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_8g063500 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_8g103245 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_8g092820 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene027907.t1 | MTR_7g013610 | 100.000 | 136 | 0 | 0 | 1 | 136 | 100 | 235 | 1.10e-95 | 275 |
| MS.gene027907.t1 | MTR_4g097175 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene027907.t1 | MTR_1g023630 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene027907.t1 | MTR_8g061940 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene027907.t1 | MTR_8g099430 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.38e-92 | 264 |
| MS.gene027907.t1 | MTR_4g097170 | 96.324 | 136 | 5 | 0 | 1 | 136 | 42 | 177 | 2.29e-91 | 262 |
| MS.gene027907.t1 | MTR_2g082310 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.38e-86 | 248 |
| MS.gene027907.t1 | MTR_2g082340 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.38e-86 | 248 |
| MS.gene027907.t1 | MTR_2g082370 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.74e-82 | 238 |
| MS.gene027907.t1 | MTR_2g082400 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 6.38e-75 | 219 |
| MS.gene027907.t1 | MTR_7g013600 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 1.01e-65 | 195 |
| MS.gene027907.t1 | MTR_7g023830 | 67.164 | 134 | 43 | 1 | 1 | 134 | 1 | 133 | 6.48e-56 | 171 |
| MS.gene027907.t1 | MTR_1g023630 | 73.504 | 117 | 15 | 3 | 1 | 111 | 1 | 107 | 3.81e-52 | 160 |
| MS.gene027907.t1 | MTR_5g024630 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.63e-45 | 144 |
| MS.gene027907.t1 | MTR_7g023880 | 58.647 | 133 | 41 | 3 | 3 | 134 | 18 | 137 | 5.46e-43 | 139 |
| MS.gene027907.t1 | MTR_5g024530 | 68.182 | 110 | 12 | 2 | 19 | 128 | 68 | 154 | 4.56e-42 | 137 |
| MS.gene027907.t1 | MTR_0197s0100 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 1.45e-40 | 132 |
| MS.gene027907.t1 | MTR_8g027840 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 4.83e-30 | 105 |
| MS.gene027907.t1 | MTR_8g105150 | 53.846 | 91 | 10 | 1 | 37 | 127 | 11 | 69 | 1.43e-25 | 92.4 |
| MS.gene027907.t1 | MTR_8g092740 | 97.368 | 38 | 1 | 0 | 99 | 136 | 118 | 155 | 4.60e-20 | 80.9 |
| MS.gene027907.t1 | MTR_5g089120 | 76.190 | 42 | 10 | 0 | 82 | 123 | 6 | 47 | 6.07e-18 | 73.6 |
| MS.gene027907.t1 | MTR_5g024600 | 62.963 | 54 | 20 | 0 | 1 | 54 | 1 | 54 | 1.76e-15 | 66.2 |
| MS.gene027907.t1 | MTR_4g128040 | 75.676 | 37 | 9 | 0 | 79 | 115 | 20 | 56 | 3.38e-15 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027907.t1 | AT5G65360 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene027907.t1 | AT5G10390 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene027907.t1 | AT5G10400 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene027907.t1 | AT3G27360 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene027907.t1 | AT1G09200 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene027907.t1 | AT5G10980 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene027907.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 6.76e-93 | 266 |
| MS.gene027907.t1 | AT5G65350 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.09e-91 | 260 |
| MS.gene027907.t1 | AT1G75600 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.20e-89 | 256 |
| MS.gene027907.t1 | AT1G13370 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.32e-89 | 254 |
| MS.gene027907.t1 | AT1G19890 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.03e-83 | 241 |
| MS.gene027907.t1 | AT5G12910 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.51e-67 | 198 |
| MS.gene027907.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene027907.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene027907.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene027907.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
Find 37 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTTGCCGGAGCAGATTTC+CGG | 0.243304 | 5.2:-18686189 | None:intergenic |
| TTGGTGGCTAGCTGCTTTCT+TGG | 0.277587 | 5.2:-18686160 | None:intergenic |
| TCACGAAGTGCAACGGTTCC+TGG | 0.291776 | 5.2:-18686241 | None:intergenic |
| TTCTTGACTCCTCCGGTTGC+CGG | 0.293014 | 5.2:-18686202 | None:intergenic |
| TGAGAAGCTCAGTGCTCTTC+TGG | 0.304985 | 5.2:-18686276 | None:intergenic |
| GCTGCTGAAGCTTATCTTGT+TGG | 0.357332 | 5.2:+18686397 | MS.gene027907:CDS |
| AGCAGATTTCCGGGCAGCTT+TGG | 0.390197 | 5.2:-18686179 | None:intergenic |
| CGGAAATCTGCTCCGGCAAC+CGG | 0.392471 | 5.2:+18686190 | MS.gene027907:CDS |
| GGTTGCCGGAGCAGATTTCC+GGG | 0.407337 | 5.2:-18686188 | None:intergenic |
| CTTTCTTGGTGCCTTTCCTC+CGG | 0.425692 | 5.2:-18686146 | None:intergenic |
| TTATCTTGTTGGTTTGTTTG+AGG | 0.428809 | 5.2:+18686408 | MS.gene027907:CDS |
| CGATTTCACGAACAAGTCTC+TGG | 0.432729 | 5.2:-18686315 | None:intergenic |
| AAACAGCGCTGCTTTGAAAT+CGG | 0.457546 | 5.2:-18686360 | None:intergenic |
| AGATTTCCGGGCAGCTTTGG+TGG | 0.468887 | 5.2:-18686176 | None:intergenic |
| AGCTAGCCACCAAAGCTGCC+CGG | 0.491500 | 5.2:+18686170 | MS.gene027907:CDS |
| CGAGCAAGTTGAATATCCTT+AGG | 0.499677 | 5.2:-18686475 | None:intergenic |
| GTGTGGCTTCTTGACTCCTC+CGG | 0.501667 | 5.2:-18686209 | None:intergenic |
| AGCTGCCCGGAAATCTGCTC+CGG | 0.505877 | 5.2:+18686183 | MS.gene027907:CDS |
| AGTAACTCTCTTAGCATGAA+TGG | 0.521941 | 5.2:-18686446 | None:intergenic |
| AGAGCACTGAGCTTCTCATA+AGG | 0.531497 | 5.2:+18686281 | MS.gene027907:CDS |
| TCGCAAATCCACCGGAGGAA+AGG | 0.532804 | 5.2:+18686135 | MS.gene027907:CDS |
| TGTTTGCTTTGTACGAGCCA+TGG | 0.537743 | 5.2:-18686110 | None:intergenic |
| GAGAGTTACTATCATGCCTA+AGG | 0.550012 | 5.2:+18686459 | MS.gene027907:CDS |
| ACTTGTTCGTGAAATCGCTC+AGG | 0.551065 | 5.2:+18686321 | MS.gene027907:CDS |
| TCTTGGTGCCTTTCCTCCGG+TGG | 0.556160 | 5.2:-18686143 | None:intergenic |
| CAGTGCTCTTCTGGTACTTG+CGG | 0.579006 | 5.2:-18686267 | None:intergenic |
| CAAACAGCTCGCAAATCCAC+CGG | 0.586123 | 5.2:+18686127 | MS.gene027907:CDS |
| TCACGAACAAGTCTCTGGAA+TGG | 0.590006 | 5.2:-18686310 | None:intergenic |
| CAACTTGCTCGCAGAATCAG+AGG | 0.592233 | 5.2:+18686487 | MS.gene027907:CDS |
| AAATCTGCTCCGGCAACCGG+AGG | 0.607496 | 5.2:+18686193 | MS.gene027907:CDS |
| TCGCAGAATCAGAGGCGAGA+GGG | 0.615698 | 5.2:+18686495 | MS.gene027907:CDS |
| CTCGCAGAATCAGAGGCGAG+AGG | 0.629911 | 5.2:+18686494 | MS.gene027907:CDS |
| AAGCCACACAGATTCCGTCC+AGG | 0.632383 | 5.2:+18686223 | MS.gene027907:CDS |
| GCGGATCTCACGAAGTGCAA+CGG | 0.633057 | 5.2:-18686248 | None:intergenic |
| GAAGTGCAACGGTTCCTGGA+CGG | 0.655834 | 5.2:-18686237 | None:intergenic |
| GTTCCTGGACGGAATCTGTG+TGG | 0.669194 | 5.2:-18686226 | None:intergenic |
| ACAGCTCGCAAATCCACCGG+AGG | 0.704783 | 5.2:+18686130 | MS.gene027907:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCTTGTTGGTTTGTTTG+AGG | + | chr5.2:18686408-18686427 | MS.gene027907:CDS | 30.0% |
| AGTAACTCTCTTAGCATGAA+TGG | - | chr5.2:18686449-18686468 | None:intergenic | 35.0% | |
| GAGAGTTACTATCATGCCTA+AGG | + | chr5.2:18686459-18686478 | MS.gene027907:CDS | 40.0% | |
| ! | CGAGCAAGTTGAATATCCTT+AGG | - | chr5.2:18686478-18686497 | None:intergenic | 40.0% |
| !! | AAACAGCGCTGCTTTGAAAT+CGG | - | chr5.2:18686363-18686382 | None:intergenic | 40.0% |
| ACTTGTTCGTGAAATCGCTC+AGG | + | chr5.2:18686321-18686340 | MS.gene027907:CDS | 45.0% | |
| CGATTTCACGAACAAGTCTC+TGG | - | chr5.2:18686318-18686337 | None:intergenic | 45.0% | |
| GCTGCTGAAGCTTATCTTGT+TGG | + | chr5.2:18686397-18686416 | MS.gene027907:CDS | 45.0% | |
| ! | TCACGAACAAGTCTCTGGAA+TGG | - | chr5.2:18686313-18686332 | None:intergenic | 45.0% |
| !! | AGAGCACTGAGCTTCTCATA+AGG | + | chr5.2:18686281-18686300 | MS.gene027907:CDS | 45.0% |
| CAAACAGCTCGCAAATCCAC+CGG | + | chr5.2:18686127-18686146 | MS.gene027907:CDS | 50.0% | |
| CAGTGCTCTTCTGGTACTTG+CGG | - | chr5.2:18686270-18686289 | None:intergenic | 50.0% | |
| TGAGAAGCTCAGTGCTCTTC+TGG | - | chr5.2:18686279-18686298 | None:intergenic | 50.0% | |
| ! | CAACTTGCTCGCAGAATCAG+AGG | + | chr5.2:18686487-18686506 | MS.gene027907:CDS | 50.0% |
| ! | TTGGTGGCTAGCTGCTTTCT+TGG | - | chr5.2:18686163-18686182 | None:intergenic | 50.0% |
| !! | CTTTCTTGGTGCCTTTCCTC+CGG | - | chr5.2:18686149-18686168 | None:intergenic | 50.0% |
| AAGCCACACAGATTCCGTCC+AGG | + | chr5.2:18686223-18686242 | MS.gene027907:CDS | 55.0% | |
| AGCAGATTTCCGGGCAGCTT+TGG | - | chr5.2:18686182-18686201 | None:intergenic | 55.0% | |
| GAAGTGCAACGGTTCCTGGA+CGG | - | chr5.2:18686240-18686259 | None:intergenic | 55.0% | |
| GCGGATCTCACGAAGTGCAA+CGG | - | chr5.2:18686251-18686270 | None:intergenic | 55.0% | |
| GTGTGGCTTCTTGACTCCTC+CGG | - | chr5.2:18686212-18686231 | None:intergenic | 55.0% | |
| GTTCCTGGACGGAATCTGTG+TGG | - | chr5.2:18686229-18686248 | None:intergenic | 55.0% | |
| TCACGAAGTGCAACGGTTCC+TGG | - | chr5.2:18686244-18686263 | None:intergenic | 55.0% | |
| TCGCAAATCCACCGGAGGAA+AGG | + | chr5.2:18686135-18686154 | MS.gene027907:CDS | 55.0% | |
| TCGCAGAATCAGAGGCGAGA+GGG | + | chr5.2:18686495-18686514 | MS.gene027907:CDS | 55.0% | |
| TTCTTGACTCCTCCGGTTGC+CGG | - | chr5.2:18686205-18686224 | None:intergenic | 55.0% | |
| ! | AGATTTCCGGGCAGCTTTGG+TGG | - | chr5.2:18686179-18686198 | None:intergenic | 55.0% |
| AAATCTGCTCCGGCAACCGG+AGG | + | chr5.2:18686193-18686212 | MS.gene027907:CDS | 60.0% | |
| ACAGCTCGCAAATCCACCGG+AGG | + | chr5.2:18686130-18686149 | MS.gene027907:CDS | 60.0% | |
| AGCTAGCCACCAAAGCTGCC+CGG | + | chr5.2:18686170-18686189 | MS.gene027907:CDS | 60.0% | |
| AGCTGCCCGGAAATCTGCTC+CGG | + | chr5.2:18686183-18686202 | MS.gene027907:CDS | 60.0% | |
| CGGAAATCTGCTCCGGCAAC+CGG | + | chr5.2:18686190-18686209 | MS.gene027907:CDS | 60.0% | |
| CGGTTGCCGGAGCAGATTTC+CGG | - | chr5.2:18686192-18686211 | None:intergenic | 60.0% | |
| CTCGCAGAATCAGAGGCGAG+AGG | + | chr5.2:18686494-18686513 | MS.gene027907:CDS | 60.0% | |
| GGTTGCCGGAGCAGATTTCC+GGG | - | chr5.2:18686191-18686210 | None:intergenic | 60.0% | |
| !! | TCTTGGTGCCTTTCCTCCGG+TGG | - | chr5.2:18686146-18686165 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 18686112 | 18686522 | 18686112 | ID=MS.gene027907 |
| chr5.2 | mRNA | 18686112 | 18686522 | 18686112 | ID=MS.gene027907.t1;Parent=MS.gene027907 |
| chr5.2 | exon | 18686112 | 18686522 | 18686112 | ID=MS.gene027907.t1.exon1;Parent=MS.gene027907.t1 |
| chr5.2 | CDS | 18686112 | 18686522 | 18686112 | ID=cds.MS.gene027907.t1;Parent=MS.gene027907.t1 |
| Gene Sequence |
| Protein sequence |