AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91631


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91631.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene91631.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene91631.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene91631.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene91631.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene91631.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene91631.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene91631.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene91631.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene91631.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene91631.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene91631.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene91631.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene91631.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene91631.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene91631.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene91631.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene91631.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene91631.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene91631.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene91631.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene91631.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene91631.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene91631.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91631.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene91631.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene91631.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene91631.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene91631.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene91631.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene91631.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene91631.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene91631.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene91631.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene91631.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene91631.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene91631.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene91631.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene91631.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene91631.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCGCAATGCGACGGTTCC+TGG 0.246474 7.4:-85491330 None:intergenic
CGATTTCACGAACCAATCTT+TGG 0.247437 7.4:-85491404 None:intergenic
TTCTTCACTCCTCCGGTTGC+CGG 0.280449 7.4:-85491291 None:intergenic
GAAACTTCCATTCCAAAGAT+TGG 0.376853 7.4:+85491392 MS.gene91631:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 7.4:-85491564 None:intergenic
AGAAAATCAGCTCCGGCAAC+CGG 0.420148 7.4:+85491279 MS.gene91631:CDS
GCTGCTGAAGCGTATCTTGT+TGG 0.422267 7.4:+85491486 MS.gene91631:CDS
GAGCAGAAACAGCACTGCTC+TGG 0.422418 7.4:-85491455 None:intergenic
CTGCTGAAGCGTATCTTGTT+GGG 0.422762 7.4:+85491487 MS.gene91631:CDS
AGAGCACCGAGCTTCTCATT+AGG 0.438663 7.4:+85491370 MS.gene91631:CDS
TGGTGGCGAGTTGTTTCCGT+GGG 0.469293 7.4:-85491248 None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG 0.469362 7.4:-85491298 None:intergenic
TCTGATTTCGCGCAATGCGA+CGG 0.516040 7.4:-85491337 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 7.4:+85491548 MS.gene91631:CDS
TCACGAACCAATCTTTGGAA+TGG 0.535409 7.4:-85491399 None:intergenic
AGCCGCAAGAAAATCAGCTC+CGG 0.539637 7.4:+85491272 MS.gene91631:CDS
AAGAAGAATCAGAGGCGAAA+GGG 0.540261 7.4:+85491584 MS.gene91631:CDS
CAAGAAGAATCAGAGGCGAA+AGG 0.575448 7.4:+85491583 MS.gene91631:CDS
TTGGTGGCGAGTTGTTTCCG+TGG 0.577381 7.4:-85491249 None:intergenic
CAAACAGCAAGAAAATCCAC+CGG 0.620184 7.4:+85491216 MS.gene91631:CDS
AAATCAGCTCCGGCAACCGG+AGG 0.628601 7.4:+85491282 MS.gene91631:CDS
AAGTTTCCTAATGAGAAGCT+CGG 0.631897 7.4:-85491376 None:intergenic
CCACCGGAGGAAAAGCCCCA+CGG 0.631926 7.4:+85491232 MS.gene91631:CDS
AAGCCACACAGATTCCGTCC+AGG 0.632383 7.4:+85491312 MS.gene91631:CDS
GCAATGCGACGGTTCCTGGA+CGG 0.638461 7.4:-85491326 None:intergenic
CAACTTGCAAGAAGAATCAG+AGG 0.660238 7.4:+85491576 MS.gene91631:CDS
GTTCCTGGACGGAATCTGTG+TGG 0.669194 7.4:-85491315 None:intergenic
ACAGCAAGAAAATCCACCGG+AGG 0.684797 7.4:+85491219 MS.gene91631:CDS
GGTGGCGAGTTGTTTCCGTG+GGG 0.739791 7.4:-85491247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGTTTCCTAATGAGAAGCT+CGG - chr7.4:85491379-85491398 None:intergenic 35.0%
GAAACTTCCATTCCAAAGAT+TGG + chr7.4:85491392-85491411 MS.gene91631:CDS 35.0%
GAGAGTTACTATTATGCCTA+AGG + chr7.4:85491548-85491567 MS.gene91631:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG - chr7.4:85491567-85491586 None:intergenic 35.0%
!!! GGATTTTCTTGCTGTTTGTT+TGG - chr7.4:85491214-85491233 None:intergenic 35.0%
AAGAAGAATCAGAGGCGAAA+GGG + chr7.4:85491584-85491603 MS.gene91631:CDS 40.0%
CAAACAGCAAGAAAATCCAC+CGG + chr7.4:85491216-85491235 MS.gene91631:CDS 40.0%
CAACTTGCAAGAAGAATCAG+AGG + chr7.4:85491576-85491595 MS.gene91631:CDS 40.0%
CGATTTCACGAACCAATCTT+TGG - chr7.4:85491407-85491426 None:intergenic 40.0%
! TCACGAACCAATCTTTGGAA+TGG - chr7.4:85491402-85491421 None:intergenic 40.0%
CAAGAAGAATCAGAGGCGAA+AGG + chr7.4:85491583-85491602 MS.gene91631:CDS 45.0%
CTGCTGAAGCGTATCTTGTT+GGG + chr7.4:85491487-85491506 MS.gene91631:CDS 45.0%
!! AGCTGATTTTCTTGCGGCTT+TGG - chr7.4:85491271-85491290 None:intergenic 45.0%
!! TGATTTTCTTGCGGCTTTGG+TGG - chr7.4:85491268-85491287 None:intergenic 45.0%
ACAGCAAGAAAATCCACCGG+AGG + chr7.4:85491219-85491238 MS.gene91631:CDS 50.0%
AGAAAATCAGCTCCGGCAAC+CGG + chr7.4:85491279-85491298 MS.gene91631:CDS 50.0%
AGCCGCAAGAAAATCAGCTC+CGG + chr7.4:85491272-85491291 MS.gene91631:CDS 50.0%
GCTGCTGAAGCGTATCTTGT+TGG + chr7.4:85491486-85491505 MS.gene91631:CDS 50.0%
TCTGATTTCGCGCAATGCGA+CGG - chr7.4:85491340-85491359 None:intergenic 50.0%
!! AGAGCACCGAGCTTCTCATT+AGG + chr7.4:85491370-85491389 MS.gene91631:CDS 50.0%
!! TGCCGGAGCTGATTTTCTTG+CGG - chr7.4:85491277-85491296 None:intergenic 50.0%
AAGCCACACAGATTCCGTCC+AGG + chr7.4:85491312-85491331 MS.gene91631:CDS 55.0%
GAGCAGAAACAGCACTGCTC+TGG - chr7.4:85491458-85491477 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG - chr7.4:85491301-85491320 None:intergenic 55.0%
GTTCCTGGACGGAATCTGTG+TGG - chr7.4:85491318-85491337 None:intergenic 55.0%
TTCTTCACTCCTCCGGTTGC+CGG - chr7.4:85491294-85491313 None:intergenic 55.0%
! TGGTGGCGAGTTGTTTCCGT+GGG - chr7.4:85491251-85491270 None:intergenic 55.0%
! TTTCCGTGGGGCTTTTCCTC+CGG - chr7.4:85491238-85491257 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTCCG+TGG - chr7.4:85491252-85491271 None:intergenic 55.0%
AAATCAGCTCCGGCAACCGG+AGG + chr7.4:85491282-85491301 MS.gene91631:CDS 60.0%
GCAATGCGACGGTTCCTGGA+CGG - chr7.4:85491329-85491348 None:intergenic 60.0%
! GGTGGCGAGTTGTTTCCGTG+GGG - chr7.4:85491250-85491269 None:intergenic 60.0%
CCACCGGAGGAAAAGCCCCA+CGG + chr7.4:85491232-85491251 MS.gene91631:CDS 65.0%
TCGCGCAATGCGACGGTTCC+TGG - chr7.4:85491333-85491352 None:intergenic 65.0%
! CCGTGGGGCTTTTCCTCCGG+TGG - chr7.4:85491235-85491254 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 85491201 85491611 85491201 ID=MS.gene91631
chr7.4 mRNA 85491201 85491611 85491201 ID=MS.gene91631.t1;Parent=MS.gene91631
chr7.4 exon 85491201 85491611 85491201 ID=MS.gene91631.t1.exon1;Parent=MS.gene91631.t1
chr7.4 CDS 85491201 85491611 85491201 ID=cds.MS.gene91631.t1;Parent=MS.gene91631.t1
Gene Sequence

>MS.gene91631

ATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCCCCACGGAAACAACTCGCCACCAAAGCCGCAAGAAAATCAGCTCCGGCAACCGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACCGTCGCATTGCGCGAAATCAGAAAGTATCAGAAGAGCACCGAGCTTCTCATTAGGAAACTTCCATTCCAAAGATTGGTTCGTGAAATCGCTCAAGATTTCAAAACTGATCTCAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCGTATCTTGTTGGGTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>MS.gene91631.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA