AlfalfaGEDB | MS.gene58116

M. sativa cultivar XinJiangDaYe / MS.gene58116

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58116.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58116.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58116.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054662	MS.gene58116	0.833448	5.30E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene58116	MS.gene47573	PPI
MS.gene58116	MS.gene94315	PPI
MS.gene58116	MS.gene027904	PPI
MS.gene58116	MS.gene059054	PPI
MS.gene58116	MS.gene47284	PPI
MS.gene58116	MS.gene55626	PPI
MS.gene58116	MS.gene60196	PPI
MS.gene58116	MS.gene61193	PPI
MS.gene58116	MS.gene04820	PPI
MS.gene58116	MS.gene069975	PPI
MS.gene58116	MS.gene34797	PPI
MS.gene58116	MS.gene78324	PPI
MS.gene58116	MS.gene072433	PPI
MS.gene70263	MS.gene58116	PPI
MS.gene58116	MS.gene26084	PPI
MS.gene58285	MS.gene58116	PPI
MS.gene58116	MS.gene017431	PPI
MS.gene58116	MS.gene78227	PPI
MS.gene58116	MS.gene70258	PPI
MS.gene58116	MS.gene066537	PPI
MS.gene58116	MS.gene65125	PPI
MS.gene58116	MS.gene056335	PPI
MS.gene58116	MS.gene011710	PPI
MS.gene58116	MS.gene021887	PPI
MS.gene58116	MS.gene23886	PPI
MS.gene58116	MS.gene013163	PPI
MS.gene58116	MS.gene88233	PPI
MS.gene58116	MS.gene046235	PPI
MS.gene58116	MS.gene29299	PPI
MS.gene58116	MS.gene32514	PPI
MS.gene027907	MS.gene58116	PPI
MS.gene58116	MS.gene015760	PPI
MS.gene58116	MS.gene94307	PPI
MS.gene067742	MS.gene58116	PPI
MS.gene58116	MS.gene89203	PPI
MS.gene58116	MS.gene63118	PPI
MS.gene58116	MS.gene066535	PPI
MS.gene58116	MS.gene78224	PPI
MS.gene58116	MS.gene038612	PPI
MS.gene61192	MS.gene58116	PPI
MS.gene58116	MS.gene033505	PPI
MS.gene28432	MS.gene58116	PPI
MS.gene58116	MS.gene29298	PPI
MS.gene58116	MS.gene44458	PPI
MS.gene58116	MS.gene042871	PPI
MS.gene95244	MS.gene58116	PPI
MS.gene58116	MS.gene78322	PPI
MS.gene58116	MS.gene46303	PPI
MS.gene58116	MS.gene98552	PPI
MS.gene58116	MS.gene28438	PPI
MS.gene58116	MS.gene64996	PPI
MS.gene78924	MS.gene58116	PPI
MS.gene58116	MS.gene066960	PPI
MS.gene58116	MS.gene063067	PPI
MS.gene58116	MS.gene06236	PPI
MS.gene58116	MS.gene066536	PPI
MS.gene58116	MS.gene072431	PPI
MS.gene39538	MS.gene58116	PPI
MS.gene78922	MS.gene58116	PPI
MS.gene58116	MS.gene78924	PPI
MS.gene071851	MS.gene58116	PPI
MS.gene58116	MS.gene03849	PPI
MS.gene58116	MS.gene04748	PPI
MS.gene28434	MS.gene58116	PPI
MS.gene016712	MS.gene58116	PPI
MS.gene014301	MS.gene58116	PPI
MS.gene58116	MS.gene35163	PPI
MS.gene000604	MS.gene58116	PPI
MS.gene58116	MS.gene49838	PPI
MS.gene58116	MS.gene29445	PPI
MS.gene58116	MS.gene32513	PPI
MS.gene79799	MS.gene58116	PPI
MS.gene58116	MS.gene81646	PPI
MS.gene58116	MS.gene030661	PPI
MS.gene58116	MS.gene66752	PPI
MS.gene58116	MS.gene26406	PPI
MS.gene58116	MS.gene70990	PPI
MS.gene58116	MS.gene24196	PPI
MS.gene58116	MS.gene068179	PPI
MS.gene60199	MS.gene58116	PPI
MS.gene58116	MS.gene027909	PPI
MS.gene58116	MS.gene70261	PPI
MS.gene58116	MS.gene009155	PPI
MS.gene020313	MS.gene58116	PPI
MS.gene58116	MS.gene25357	PPI
MS.gene58116	MS.gene018762	PPI
MS.gene064876	MS.gene58116	PPI
MS.gene44458	MS.gene58116	PPI
MS.gene58116	MS.gene97703	PPI
MS.gene58116	MS.gene64998	PPI
MS.gene58116	MS.gene78323	PPI
MS.gene58116	MS.gene91631	PPI
MS.gene58116	MS.gene58114	PPI
MS.gene58116	MS.gene030664	PPI
MS.gene58116	MS.gene50129	PPI
MS.gene58116	MS.gene034255	PPI
MS.gene58116	MS.gene014181	PPI
MS.gene58116	MS.gene030666	PPI
MS.gene58116	MS.gene66836	PPI
MS.gene58116	MS.gene60200	PPI
MS.gene97701	MS.gene58116	PPI
MS.gene58116	MS.gene82109	PPI
MS.gene58116	MS.gene053230	PPI
MS.gene58116	MS.gene05905	PPI
MS.gene58116	MS.gene66051	PPI
MS.gene58116	MS.gene024064	PPI
MS.gene58116	MS.gene64164	PPI
MS.gene58116	MS.gene41679	PPI
MS.gene58116	MS.gene36496	PPI
MS.gene049111	MS.gene58116	PPI
MS.gene58116	MS.gene057458	PPI
MS.gene58116	MS.gene29521	PPI
MS.gene58116	MS.gene028296	PPI
MS.gene58116	MS.gene07616	PPI
MS.gene58116	MS.gene055512	PPI
MS.gene58116	MS.gene39538	PPI
MS.gene58116	MS.gene58112	PPI
MS.gene58116	MS.gene29300	PPI
MS.gene58116	MS.gene070538	PPI
MS.gene44457	MS.gene58116	PPI
MS.gene58116	MS.gene071851	PPI
MS.gene58116	MS.gene072432	PPI
MS.gene68113	MS.gene58116	PPI
MS.gene66051	MS.gene58116	PPI
MS.gene011711	MS.gene58116	PPI
MS.gene58116	MS.gene52605	PPI
MS.gene87898	MS.gene58116	PPI
MS.gene58116	MS.gene00233	PPI
MS.gene58116	MS.gene68716	PPI
MS.gene58116	MS.gene068178	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58116.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene58116.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene58116.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene58116.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene58116.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene58116.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene58116.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene58116.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene58116.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene58116.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene58116.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene58116.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene58116.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene58116.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene58116.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene58116.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene58116.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene58116.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene58116.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene58116.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene58116.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene58116.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene58116.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene58116.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58116.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene58116.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene58116.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene58116.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene58116.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene58116.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene58116.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene58116.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene58116.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene58116.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene58116.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene58116.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene58116.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene58116.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene58116.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene58116.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 31 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGGTTGCCAGTTGCTTC+CGG	0.239809	4.2:+9571741	None:intergenic
CAGTGCTGTTTCTGCTCTTC+AGG	0.258870	4.2:-9571531	MS.gene58116:CDS
TTCTTCACTCCTCCGGTTGC+CGG	0.280449	4.2:+9571701	None:intergenic
TTTGGTTGCCAGTTGCTTCC+GGG	0.293951	4.2:+9571742	None:intergenic
TCTCTCAAAGCAACAGTTCC+AGG	0.333816	4.2:+9571662	None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG	0.369838	4.2:-9571510	MS.gene58116:CDS
CAAGGCTCCCCGGAAGCAAC+TGG	0.376550	4.2:-9571750	MS.gene58116:CDS
CGAAAATCAGCTCCGGCAAC+CGG	0.399036	4.2:-9571713	MS.gene58116:CDS
GAGCAGAAACAGCACTGCTC+TGG	0.422418	4.2:+9571537	None:intergenic
GACGTTGGAATGGAAGTTTA+CGG	0.458530	4.2:+9571603	None:intergenic
CTTGCAAGCTGAATGTCCTT+AGG	0.462646	4.2:+9571428	None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG	0.469362	4.2:+9571694	None:intergenic
GCAGCTGAAGCTTACTTGGT+TGG	0.472209	4.2:-9571506	MS.gene58116:CDS
CCACCGGCGGCAAGGCTCCC+CGG	0.480154	4.2:-9571760	MS.gene58116:CDS
TCGCAAGTCCACCGGCGGCA+AGG	0.488583	4.2:-9571768	MS.gene58116:CDS
TTGGTTGCCAGTTGCTTCCG+GGG	0.522282	4.2:+9571743	None:intergenic
AGCCGCCCGAAAATCAGCTC+CGG	0.530760	4.2:-9571720	MS.gene58116:CDS
CTTCCGGGGAGCCTTGCCGC+CGG	0.543290	4.2:+9571757	None:intergenic
CCGGGGAGCCTTGCCGCCGG+TGG	0.548377	4.2:+9571760	None:intergenic
GGACTTGCGAGCAGTCTGCT+TGG	0.551577	4.2:+9571781	None:intergenic
CAGACTGCTCGCAAGTCCAC+CGG	0.562052	4.2:-9571776	MS.gene58116:CDS
GAGAGTTACAATCATGCCTA+AGG	0.570792	4.2:-9571444	MS.gene58116:CDS
AAGAAGAATCAGAGGAGAGA+GGG	0.573886	4.2:-9571408	MS.gene58116:CDS
TCACGAACAAGACGTTGGAA+TGG	0.598115	4.2:+9571593	None:intergenic
CAAGAAGAATCAGAGGAGAG+AGG	0.603703	4.2:-9571409	MS.gene58116:CDS
CGATTTCACGAACAAGACGT+TGG	0.613448	4.2:+9571588	None:intergenic
CAGCTTGCAAGAAGAATCAG+AGG	0.621034	4.2:-9571416	MS.gene58116:CDS
AAATCAGCTCCGGCAACCGG+AGG	0.622487	4.2:-9571710	MS.gene58116:CDS
ACTGCTCGCAAGTCCACCGG+CGG	0.647164	4.2:-9571773	MS.gene58116:CDS
AAGCCACACAGATTCAGACC+TGG	0.672238	4.2:-9571680	MS.gene58116:CDS
GTTCCAGGTCTGAATCTGTG+TGG	0.712872	4.2:+9571677	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGAATCAGAGGAGAGA+GGG	-	chr4.2:9571786-9571805	MS.gene58116:CDS	40.0%
	GACGTTGGAATGGAAGTTTA+CGG	+	chr4.2:9571594-9571613	None:intergenic	40.0%
	GAGAGTTACAATCATGCCTA+AGG	-	chr4.2:9571750-9571769	MS.gene58116:CDS	40.0%
	CAAGAAGAATCAGAGGAGAG+AGG	-	chr4.2:9571785-9571804	MS.gene58116:CDS	45.0%
	CAGCTTGCAAGAAGAATCAG+AGG	-	chr4.2:9571778-9571797	MS.gene58116:CDS	45.0%
	CGATTTCACGAACAAGACGT+TGG	+	chr4.2:9571609-9571628	None:intergenic	45.0%
	CTTGCAAGCTGAATGTCCTT+AGG	+	chr4.2:9571769-9571788	None:intergenic	45.0%
	TCACGAACAAGACGTTGGAA+TGG	+	chr4.2:9571604-9571623	None:intergenic	45.0%
	TCTCTCAAAGCAACAGTTCC+AGG	+	chr4.2:9571535-9571554	None:intergenic	45.0%
	AAGCCACACAGATTCAGACC+TGG	-	chr4.2:9571514-9571533	MS.gene58116:CDS	50.0%
	CTTTGGTTGCCAGTTGCTTC+CGG	+	chr4.2:9571456-9571475	None:intergenic	50.0%
	GCAGCTGAAGCTTACTTGGT+TGG	-	chr4.2:9571688-9571707	MS.gene58116:CDS	50.0%
	GGAAGCAGCTGAAGCTTACT+TGG	-	chr4.2:9571684-9571703	MS.gene58116:CDS	50.0%
	GTTCCAGGTCTGAATCTGTG+TGG	+	chr4.2:9571520-9571539	None:intergenic	50.0%
	TTTGGTTGCCAGTTGCTTCC+GGG	+	chr4.2:9571455-9571474	None:intergenic	50.0%
!!	CAGTGCTGTTTCTGCTCTTC+AGG	-	chr4.2:9571663-9571682	MS.gene58116:CDS	50.0%
	CGAAAATCAGCTCCGGCAAC+CGG	-	chr4.2:9571481-9571500	MS.gene58116:CDS	55.0%
	GAGCAGAAACAGCACTGCTC+TGG	+	chr4.2:9571660-9571679	None:intergenic	55.0%
	GTGTGGCTTCTTCACTCCTC+CGG	+	chr4.2:9571503-9571522	None:intergenic	55.0%
	TTCTTCACTCCTCCGGTTGC+CGG	+	chr4.2:9571496-9571515	None:intergenic	55.0%
	TTGGTTGCCAGTTGCTTCCG+GGG	+	chr4.2:9571454-9571473	None:intergenic	55.0%
!!	AGCTGATTTTCGGGCGGCTT+TGG	+	chr4.2:9571473-9571492	None:intergenic	55.0%
!!	CGGTTGCCGGAGCTGATTTT+CGG	+	chr4.2:9571483-9571502	None:intergenic	55.0%
!!	GGTTGCCGGAGCTGATTTTC+GGG	+	chr4.2:9571482-9571501	None:intergenic	55.0%
	AAATCAGCTCCGGCAACCGG+AGG	-	chr4.2:9571484-9571503	MS.gene58116:CDS	60.0%
	AGCCGCCCGAAAATCAGCTC+CGG	-	chr4.2:9571474-9571493	MS.gene58116:CDS	60.0%
	CAGACTGCTCGCAAGTCCAC+CGG	-	chr4.2:9571418-9571437	MS.gene58116:CDS	60.0%
	GGACTTGCGAGCAGTCTGCT+TGG	+	chr4.2:9571416-9571435	None:intergenic	60.0%
!!	TGCCGGAGCTGATTTTCGGG+CGG	+	chr4.2:9571479-9571498	None:intergenic	60.0%
	ACTGCTCGCAAGTCCACCGG+CGG	-	chr4.2:9571421-9571440	MS.gene58116:CDS	65.0%
	CAAGGCTCCCCGGAAGCAAC+TGG	-	chr4.2:9571444-9571463	MS.gene58116:CDS	65.0%
	TCGCAAGTCCACCGGCGGCA+AGG	-	chr4.2:9571426-9571445	MS.gene58116:CDS	70.0%
	CTTCCGGGGAGCCTTGCCGC+CGG	+	chr4.2:9571440-9571459	None:intergenic	75.0%
!	CCACCGGCGGCAAGGCTCCC+CGG	-	chr4.2:9571434-9571453	MS.gene58116:CDS	80.0%
!	CCGGGGAGCCTTGCCGCCGG+TGG	+	chr4.2:9571437-9571456	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	9571403	9571813	9571403	ID=MS.gene58116
chr4.2	mRNA	9571403	9571813	9571403	ID=MS.gene58116.t1;Parent=MS.gene58116
chr4.2	exon	9571403	9571813	9571403	ID=MS.gene58116.t1.exon1;Parent=MS.gene58116.t1
chr4.2	CDS	9571403	9571813	9571403	ID=cds.MS.gene58116.t1;Parent=MS.gene58116.t1

Gene Sequence

>MS.gene58116

ATGGCACGTACCAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAACTGGCAACCAAAGCCGCCCGAAAATCAGCTCCGGCAACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACTGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAATCTGTGCGCAATTCATGCTAAGAGAGTTACAATCATGCCTAAGGACATTCAGCTTGCAAGAAGAATCAGAGGAGAGAGGGCTTAG

Protein sequence

>MS.gene58116.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA