AlfalfaGEDB | MS.gene030666

M. sativa cultivar XinJiangDaYe / MS.gene030666

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030666.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030666.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030666.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene030666	MS.gene014181	PPI
MS.gene030666	MS.gene49838	PPI
MS.gene030666	MS.gene32513	PPI
MS.gene030666	MS.gene64164	PPI
MS.gene030666	MS.gene41679	PPI
MS.gene030666	MS.gene81646	PPI
MS.gene030666	MS.gene030661	PPI
MS.gene030666	MS.gene66752	PPI
MS.gene030666	MS.gene26406	PPI
MS.gene030666	MS.gene70990	PPI
MS.gene030666	MS.gene24196	PPI
MS.gene030666	MS.gene068179	PPI
MS.gene60199	MS.gene030666	PPI
MS.gene021887	MS.gene030666	PPI
MS.gene030666	MS.gene027909	PPI
MS.gene030666	MS.gene70261	PPI
MS.gene030666	MS.gene009155	PPI
MS.gene030666	MS.gene25357	PPI
MS.gene32514	MS.gene030666	PPI
MS.gene030666	MS.gene018762	PPI
MS.gene053230	MS.gene030666	PPI
MS.gene064876	MS.gene030666	PPI
MS.gene44458	MS.gene030666	PPI
MS.gene030666	MS.gene97703	PPI
MS.gene028296	MS.gene030666	PPI
MS.gene030666	MS.gene64998	PPI
MS.gene04820	MS.gene030666	PPI
MS.gene030666	MS.gene78323	PPI
MS.gene030666	MS.gene58114	PPI
MS.gene030666	MS.gene030664	PPI
MS.gene030666	MS.gene66836	PPI
MS.gene030666	MS.gene60200	PPI
MS.gene030666	MS.gene55626	PPI
MS.gene97701	MS.gene030666	PPI
MS.gene030666	MS.gene82109	PPI
MS.gene030666	MS.gene05905	PPI
MS.gene030666	MS.gene024064	PPI
MS.gene030666	MS.gene36496	PPI
MS.gene049111	MS.gene030666	PPI
MS.gene030666	MS.gene057458	PPI
MS.gene030666	MS.gene29521	PPI
MS.gene70263	MS.gene030666	PPI
MS.gene030666	MS.gene07616	PPI
MS.gene030666	MS.gene055512	PPI
MS.gene030666	MS.gene056335	PPI
MS.gene030666	MS.gene58112	PPI
MS.gene030666	MS.gene29300	PPI
MS.gene030666	MS.gene070538	PPI
MS.gene44457	MS.gene030666	PPI
MS.gene030666	MS.gene072432	PPI
MS.gene68113	MS.gene030666	PPI
MS.gene030666	MS.gene89203	PPI
MS.gene66051	MS.gene030666	PPI
MS.gene011711	MS.gene030666	PPI
MS.gene87898	MS.gene030666	PPI
MS.gene030666	MS.gene00233	PPI
MS.gene030666	MS.gene63118	PPI
MS.gene030666	MS.gene68716	PPI
MS.gene030666	MS.gene068178	PPI
MS.gene030666	MS.gene47573	PPI
MS.gene030666	MS.gene94315	PPI
MS.gene030666	MS.gene027904	PPI
MS.gene030666	MS.gene059054	PPI
MS.gene046235	MS.gene030666	PPI
MS.gene030666	MS.gene47284	PPI
MS.gene030666	MS.gene60196	PPI
MS.gene030666	MS.gene61193	PPI
MS.gene030666	MS.gene069975	PPI
MS.gene030666	MS.gene34797	PPI
MS.gene030666	MS.gene78324	PPI
MS.gene030666	MS.gene072433	PPI
MS.gene030666	MS.gene26084	PPI
MS.gene030666	MS.gene017431	PPI
MS.gene030666	MS.gene78227	PPI
MS.gene030666	MS.gene70258	PPI
MS.gene58285	MS.gene030666	PPI
MS.gene030666	MS.gene066537	PPI
MS.gene030666	MS.gene65125	PPI
MS.gene030666	MS.gene011710	PPI
MS.gene030666	MS.gene021887	PPI
MS.gene030666	MS.gene23886	PPI
MS.gene030666	MS.gene013163	PPI
MS.gene030666	MS.gene29299	PPI
MS.gene030666	MS.gene015760	PPI
MS.gene027907	MS.gene030666	PPI
MS.gene067742	MS.gene030666	PPI
MS.gene014301	MS.gene030666	PPI
MS.gene030666	MS.gene35163	PPI
MS.gene030666	MS.gene066535	PPI
MS.gene000604	MS.gene030666	PPI
MS.gene030666	MS.gene29445	PPI
MS.gene58116	MS.gene030666	PPI
MS.gene030666	MS.gene78224	PPI
MS.gene030666	MS.gene038612	PPI
MS.gene61192	MS.gene030666	PPI
MS.gene030666	MS.gene033505	PPI
MS.gene28432	MS.gene030666	PPI
MS.gene79799	MS.gene030666	PPI
MS.gene88233	MS.gene030666	PPI
MS.gene030666	MS.gene29298	PPI
MS.gene030666	MS.gene042871	PPI
MS.gene06236	MS.gene030666	PPI
MS.gene030666	MS.gene78322	PPI
MS.gene030666	MS.gene46303	PPI
MS.gene95244	MS.gene030666	PPI
MS.gene030666	MS.gene28438	PPI
MS.gene94307	MS.gene030666	PPI
MS.gene030666	MS.gene64996	PPI
MS.gene030666	MS.gene066960	PPI
MS.gene78924	MS.gene030666	PPI
MS.gene52605	MS.gene030666	PPI
MS.gene030666	MS.gene063067	PPI
MS.gene020313	MS.gene030666	PPI
MS.gene98552	MS.gene030666	PPI
MS.gene030666	MS.gene066536	PPI
MS.gene030666	MS.gene072431	PPI
MS.gene39538	MS.gene030666	PPI
MS.gene78922	MS.gene030666	PPI
MS.gene030666	MS.gene91631	PPI
MS.gene071851	MS.gene030666	PPI
MS.gene030666	MS.gene03849	PPI
MS.gene030666	MS.gene04748	PPI
MS.gene030666	MS.gene50129	PPI
MS.gene28434	MS.gene030666	PPI
MS.gene016712	MS.gene030666	PPI
MS.gene030666	MS.gene034255	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030666.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene030666.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene030666.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene030666.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene030666.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene030666.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene030666.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene030666.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene030666.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene030666.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene030666.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene030666.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene030666.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene030666.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene030666.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene030666.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene030666.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene030666.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene030666.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene030666.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene030666.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene030666.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene030666.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene030666.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030666.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene030666.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene030666.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene030666.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene030666.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene030666.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene030666.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene030666.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene030666.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene030666.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene030666.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene030666.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene030666.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene030666.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene030666.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene030666.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 31 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGGTTGCCAGTTGCTTC+CGG	0.239809	4.3:+8749915	None:intergenic
CAGTGCTGTTTCTGCTCTTC+AGG	0.258870	4.3:-8749705	MS.gene030666:CDS
TTCTTCACTCCTCCGGTTGC+CGG	0.280449	4.3:+8749875	None:intergenic
TTTGGTTGCCAGTTGCTTCC+GGG	0.293951	4.3:+8749916	None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG	0.333816	4.3:+8749836	None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG	0.369838	4.3:-8749684	MS.gene030666:CDS
CAAGGCTCCCCGGAAGCAAC+TGG	0.376550	4.3:-8749924	MS.gene030666:CDS
CGAAAATCAGCTCCGGCAAC+CGG	0.399036	4.3:-8749887	MS.gene030666:CDS
GAGCAGAAACAGCACTGCTC+TGG	0.422418	4.3:+8749711	None:intergenic
GACGTTGGAATGGAAGTTTA+CGG	0.458530	4.3:+8749777	None:intergenic
CTTGCAAGCTGAATGTCCTT+AGG	0.462646	4.3:+8749602	None:intergenic
GTGTGGCTTCTTCACTCCTC+CGG	0.469362	4.3:+8749868	None:intergenic
GCAGCTGAAGCTTACTTGGT+TGG	0.472209	4.3:-8749680	MS.gene030666:CDS
CCACCGGCGGCAAGGCTCCC+CGG	0.480154	4.3:-8749934	MS.gene030666:CDS
TCGCAAGTCCACCGGCGGCA+AGG	0.488583	4.3:-8749942	MS.gene030666:CDS
TTGGTTGCCAGTTGCTTCCG+GGG	0.522282	4.3:+8749917	None:intergenic
AGCCGCCCGAAAATCAGCTC+CGG	0.530760	4.3:-8749894	MS.gene030666:CDS
CTTCCGGGGAGCCTTGCCGC+CGG	0.543290	4.3:+8749931	None:intergenic
CCGGGGAGCCTTGCCGCCGG+TGG	0.548377	4.3:+8749934	None:intergenic
GGACTTGCGAGCAGTCTGCT+TGG	0.551577	4.3:+8749955	None:intergenic
CAGACTGCTCGCAAGTCCAC+CGG	0.562052	4.3:-8749950	MS.gene030666:CDS
GAGAGTTACAATCATGCCTA+AGG	0.570792	4.3:-8749618	MS.gene030666:CDS
AAGAAGAATCAGAGGAGAGA+GGG	0.573886	4.3:-8749582	MS.gene030666:CDS
TCACGAACAAGACGTTGGAA+TGG	0.598115	4.3:+8749767	None:intergenic
CAAGAAGAATCAGAGGAGAG+AGG	0.603703	4.3:-8749583	MS.gene030666:CDS
CGATTTCACGAACAAGACGT+TGG	0.613448	4.3:+8749762	None:intergenic
CAGCTTGCAAGAAGAATCAG+AGG	0.621034	4.3:-8749590	MS.gene030666:CDS
AAATCAGCTCCGGCAACCGG+AGG	0.622487	4.3:-8749884	MS.gene030666:CDS
ACTGCTCGCAAGTCCACCGG+CGG	0.647164	4.3:-8749947	MS.gene030666:CDS
AAGCCACACAGATTCAGACC+TGG	0.672238	4.3:-8749854	MS.gene030666:CDS
GTTCCAGGTCTGAATCTGTG+TGG	0.688220	4.3:+8749851	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGAATCAGAGGAGAGA+GGG	-	chr4.3:8749960-8749979	MS.gene030666:CDS	40.0%
	GACGTTGGAATGGAAGTTTA+CGG	+	chr4.3:8749768-8749787	None:intergenic	40.0%
	GAGAGTTACAATCATGCCTA+AGG	-	chr4.3:8749924-8749943	MS.gene030666:CDS	40.0%
	CAAGAAGAATCAGAGGAGAG+AGG	-	chr4.3:8749959-8749978	MS.gene030666:CDS	45.0%
	CAGCTTGCAAGAAGAATCAG+AGG	-	chr4.3:8749952-8749971	MS.gene030666:CDS	45.0%
	CGATTTCACGAACAAGACGT+TGG	+	chr4.3:8749783-8749802	None:intergenic	45.0%
	CTTGCAAGCTGAATGTCCTT+AGG	+	chr4.3:8749943-8749962	None:intergenic	45.0%
	TCACGAACAAGACGTTGGAA+TGG	+	chr4.3:8749778-8749797	None:intergenic	45.0%
	TCTCTCAAAGCAACTGTTCC+AGG	+	chr4.3:8749709-8749728	None:intergenic	45.0%
	AAGCCACACAGATTCAGACC+TGG	-	chr4.3:8749688-8749707	MS.gene030666:CDS	50.0%
	CTTTGGTTGCCAGTTGCTTC+CGG	+	chr4.3:8749630-8749649	None:intergenic	50.0%
	GCAGCTGAAGCTTACTTGGT+TGG	-	chr4.3:8749862-8749881	MS.gene030666:CDS	50.0%
	GGAAGCAGCTGAAGCTTACT+TGG	-	chr4.3:8749858-8749877	MS.gene030666:CDS	50.0%
	GTTCCAGGTCTGAATCTGTG+TGG	+	chr4.3:8749694-8749713	None:intergenic	50.0%
	TTTGGTTGCCAGTTGCTTCC+GGG	+	chr4.3:8749629-8749648	None:intergenic	50.0%
!!	CAGTGCTGTTTCTGCTCTTC+AGG	-	chr4.3:8749837-8749856	MS.gene030666:CDS	50.0%
	CGAAAATCAGCTCCGGCAAC+CGG	-	chr4.3:8749655-8749674	MS.gene030666:CDS	55.0%
	GAGCAGAAACAGCACTGCTC+TGG	+	chr4.3:8749834-8749853	None:intergenic	55.0%
	GTGTGGCTTCTTCACTCCTC+CGG	+	chr4.3:8749677-8749696	None:intergenic	55.0%
	TTCTTCACTCCTCCGGTTGC+CGG	+	chr4.3:8749670-8749689	None:intergenic	55.0%
	TTGGTTGCCAGTTGCTTCCG+GGG	+	chr4.3:8749628-8749647	None:intergenic	55.0%
!!	AGCTGATTTTCGGGCGGCTT+TGG	+	chr4.3:8749647-8749666	None:intergenic	55.0%
!!	CGGTTGCCGGAGCTGATTTT+CGG	+	chr4.3:8749657-8749676	None:intergenic	55.0%
!!	GGTTGCCGGAGCTGATTTTC+GGG	+	chr4.3:8749656-8749675	None:intergenic	55.0%
	AAATCAGCTCCGGCAACCGG+AGG	-	chr4.3:8749658-8749677	MS.gene030666:CDS	60.0%
	AGCCGCCCGAAAATCAGCTC+CGG	-	chr4.3:8749648-8749667	MS.gene030666:CDS	60.0%
	CAGACTGCTCGCAAGTCCAC+CGG	-	chr4.3:8749592-8749611	MS.gene030666:CDS	60.0%
	GGACTTGCGAGCAGTCTGCT+TGG	+	chr4.3:8749590-8749609	None:intergenic	60.0%
!!	TGCCGGAGCTGATTTTCGGG+CGG	+	chr4.3:8749653-8749672	None:intergenic	60.0%
	ACTGCTCGCAAGTCCACCGG+CGG	-	chr4.3:8749595-8749614	MS.gene030666:CDS	65.0%
	CAAGGCTCCCCGGAAGCAAC+TGG	-	chr4.3:8749618-8749637	MS.gene030666:CDS	65.0%
	TCGCAAGTCCACCGGCGGCA+AGG	-	chr4.3:8749600-8749619	MS.gene030666:CDS	70.0%
	CTTCCGGGGAGCCTTGCCGC+CGG	+	chr4.3:8749614-8749633	None:intergenic	75.0%
!	CCACCGGCGGCAAGGCTCCC+CGG	-	chr4.3:8749608-8749627	MS.gene030666:CDS	80.0%
!	CCGGGGAGCCTTGCCGCCGG+TGG	+	chr4.3:8749611-8749630	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	8749577	8749987	8749577	ID=MS.gene030666
chr4.3	mRNA	8749577	8749987	8749577	ID=MS.gene030666.t1;Parent=MS.gene030666
chr4.3	exon	8749577	8749987	8749577	ID=MS.gene030666.t1.exon1;Parent=MS.gene030666.t1
chr4.3	CDS	8749577	8749987	8749577	ID=cds.MS.gene030666.t1;Parent=MS.gene030666.t1

Gene Sequence

>MS.gene030666

ATGGCACGTACCAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAACTGGCAACCAAAGCCGCCCGAAAATCAGCTCCGGCAACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAATCTGTGCGCAATTCATGCTAAGAGAGTTACAATCATGCCTAAGGACATTCAGCTTGCAAGAAGAATCAGAGGAGAGAGGGCTTAG

Protein sequence

>MS.gene030666.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA