AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58114


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58114.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene58114.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene58114.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene58114.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene58114.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene58114.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene58114.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene58114.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene58114.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene58114.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene58114.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene58114.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene58114.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene58114.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene58114.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene58114.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene58114.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene58114.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene58114.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene58114.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene58114.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene58114.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene58114.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene58114.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58114.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene58114.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene58114.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene58114.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene58114.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene58114.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene58114.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene58114.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene58114.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene58114.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene58114.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene58114.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene58114.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene58114.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene58114.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene58114.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTGGCGAGCTGCTTC+CGG 0.229871 4.2:-9550557 None:intergenic
CTTTGTGGCGAGCTGCTTCC+GGG 0.231058 4.2:-9550556 None:intergenic
CAGTGCTGTTTCTGCTCTTC+AGG 0.258870 4.2:+9550767 MS.gene58114:CDS
TCTTGCAAGTTGAATGTCCT+TGG 0.320782 4.2:-9550871 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4.2:-9550636 None:intergenic
CTTGCAAGTTGAATGTCCTT+GGG 0.355623 4.2:-9550870 None:intergenic
GGAAGCAGCTGAAGCTTACT+TGG 0.369838 4.2:+9550788 MS.gene58114:CDS
GAGCAGAAACAGCACTGCTC+TGG 0.422418 4.2:-9550761 None:intergenic
TGATTTCCGAGCGGCCTTTG+TGG 0.424857 4.2:-9550571 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 4.2:-9550597 None:intergenic
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4.2:-9550695 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.461897 4.2:+9550585 MS.gene58114:CDS
GCAGCTGAAGCTTACTTGGT+TGG 0.472209 4.2:+9550792 MS.gene58114:CDS
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4.2:+9550538 MS.gene58114:CDS
AGCTCGCCACAAAGGCCGCT+CGG 0.491073 4.2:+9550565 MS.gene58114:CDS
GGCCGCTCGGAAATCAGCTC+CGG 0.498921 4.2:+9550578 MS.gene58114:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.499925 4.2:-9550604 None:intergenic
CTTCCGGGGAGCCTTGCCGC+CGG 0.507682 4.2:-9550541 None:intergenic
GGACTTGCGAGCTGTTTGCT+TGG 0.512299 4.2:-9550517 None:intergenic
TCGCAAGTCCACCGGCGGCA+AGG 0.530180 4.2:+9550530 MS.gene58114:CDS
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4.2:-9550538 None:intergenic
CAAACAGCTCGCAAGTCCAC+CGG 0.573307 4.2:+9550522 MS.gene58114:CDS
TTTGTGGCGAGCTGCTTCCG+GGG 0.576886 4.2:-9550555 None:intergenic
AAGAAGAATCAGAGGCGAGA+GGG 0.597241 4.2:+9550890 MS.gene58114:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4.2:-9550705 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 4.2:+9550588 MS.gene58114:CDS
CCGGAAGCAGCTCGCCACAA+AGG 0.611340 4.2:+9550557 MS.gene58114:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4.2:-9550710 None:intergenic
GAGAGTTACAATCATGCCCA+AGG 0.637894 4.2:+9550854 MS.gene58114:CDS
TCTTAGCATGAATTGCACAA+AGG 0.643725 4.2:-9550833 None:intergenic
CAAGAAGAATCAGAGGCGAG+AGG 0.648850 4.2:+9550889 MS.gene58114:CDS
ACAGCTCGCAAGTCCACCGG+CGG 0.655092 4.2:+9550525 MS.gene58114:CDS
CAACTTGCAAGAAGAATCAG+AGG 0.655500 4.2:+9550882 MS.gene58114:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4.2:+9550618 MS.gene58114:CDS
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4.2:-9550621 None:intergenic
CGCCGGAGCTGATTTCCGAG+CGG 0.728257 4.2:-9550580 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCTTAGCATGAATTGCACAA+AGG - chr4.2:9550836-9550855 None:intergenic 35.0%
CAACTTGCAAGAAGAATCAG+AGG + chr4.2:9550882-9550901 MS.gene58114:CDS 40.0%
GACGTTGGAATGGAAGTTTA+CGG - chr4.2:9550698-9550717 None:intergenic 40.0%
! CTTGCAAGTTGAATGTCCTT+GGG - chr4.2:9550873-9550892 None:intergenic 40.0%
! TCTTGCAAGTTGAATGTCCT+TGG - chr4.2:9550874-9550893 None:intergenic 40.0%
AAGAAGAATCAGAGGCGAGA+GGG + chr4.2:9550890-9550909 MS.gene58114:CDS 45.0%
CGATTTCACGAACAAGACGT+TGG - chr4.2:9550713-9550732 None:intergenic 45.0%
GAGAGTTACAATCATGCCCA+AGG + chr4.2:9550854-9550873 MS.gene58114:CDS 45.0%
TCACGAACAAGACGTTGGAA+TGG - chr4.2:9550708-9550727 None:intergenic 45.0%
TCTCTCAAAGCAACTGTTCC+AGG - chr4.2:9550639-9550658 None:intergenic 45.0%
AAGCCACACAGATTCAGACC+TGG + chr4.2:9550618-9550637 MS.gene58114:CDS 50.0%
CAAGAAGAATCAGAGGCGAG+AGG + chr4.2:9550889-9550908 MS.gene58114:CDS 50.0%
GCAGCTGAAGCTTACTTGGT+TGG + chr4.2:9550792-9550811 MS.gene58114:CDS 50.0%
GGAAGCAGCTGAAGCTTACT+TGG + chr4.2:9550788-9550807 MS.gene58114:CDS 50.0%
GTTCCAGGTCTGAATCTGTG+TGG - chr4.2:9550624-9550643 None:intergenic 50.0%
!! CAGTGCTGTTTCTGCTCTTC+AGG + chr4.2:9550767-9550786 MS.gene58114:CDS 50.0%
CAAACAGCTCGCAAGTCCAC+CGG + chr4.2:9550522-9550541 MS.gene58114:CDS 55.0%
GAGCAGAAACAGCACTGCTC+TGG - chr4.2:9550764-9550783 None:intergenic 55.0%
GGACTTGCGAGCTGTTTGCT+TGG - chr4.2:9550520-9550539 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG - chr4.2:9550607-9550626 None:intergenic 55.0%
TGATTTCCGAGCGGCCTTTG+TGG - chr4.2:9550574-9550593 None:intergenic 55.0%
CCTTTGTGGCGAGCTGCTTC+CGG - chr4.2:9550560-9550579 None:intergenic 60.0%
CTTTGTGGCGAGCTGCTTCC+GGG - chr4.2:9550559-9550578 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG - chr4.2:9550600-9550619 None:intergenic 60.0%
TTTGTGGCGAGCTGCTTCCG+GGG - chr4.2:9550558-9550577 None:intergenic 60.0%
AAATCAGCTCCGGCGACCGG+AGG + chr4.2:9550588-9550607 MS.gene58114:CDS 65.0%
ACAGCTCGCAAGTCCACCGG+CGG + chr4.2:9550525-9550544 MS.gene58114:CDS 65.0%
AGCTCGCCACAAAGGCCGCT+CGG + chr4.2:9550565-9550584 MS.gene58114:CDS 65.0%
CCGGAAGCAGCTCGCCACAA+AGG + chr4.2:9550557-9550576 MS.gene58114:CDS 65.0%
CGGAAATCAGCTCCGGCGAC+CGG + chr4.2:9550585-9550604 MS.gene58114:CDS 65.0%
GGCCGCTCGGAAATCAGCTC+CGG + chr4.2:9550578-9550597 MS.gene58114:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG - chr4.2:9550583-9550602 None:intergenic 65.0%
TCGCAAGTCCACCGGCGGCA+AGG + chr4.2:9550530-9550549 MS.gene58114:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG - chr4.2:9550544-9550563 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG + chr4.2:9550538-9550557 MS.gene58114:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG - chr4.2:9550541-9550560 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 9550507 9550917 9550507 ID=MS.gene58114
chr4.2 mRNA 9550507 9550917 9550507 ID=MS.gene58114.t1;Parent=MS.gene58114
chr4.2 exon 9550507 9550917 9550507 ID=MS.gene58114.t1.exon1;Parent=MS.gene58114.t1
chr4.2 CDS 9550507 9550917 9550507 ID=cds.MS.gene58114.t1;Parent=MS.gene58114.t1
Gene Sequence

>MS.gene58114

ATGGCACGTACCAAGCAAACAGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAGCTCGCCACAAAGGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTTAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAGGAAGCAGCTGAAGCTTACTTGGTTGGACTCTTTGAAGACACAAACCTTTGTGCAATTCATGCTAAGAGAGTTACAATCATGCCCAAGGACATTCAACTTGCAAGAAGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MS.gene58114.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA