AlfalfaGEDB | MS.gene29445

M. sativa cultivar XinJiangDaYe / MS.gene29445

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29445.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29445.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29445.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene29445	MS.gene89203	PPI
MS.gene29445	MS.gene63118	PPI
MS.gene29445	MS.gene68716	PPI
MS.gene046235	MS.gene29445	PPI
MS.gene29445	MS.gene78224	PPI
MS.gene29445	MS.gene038612	PPI
MS.gene29445	MS.gene033505	PPI
MS.gene60200	MS.gene29445	PPI
MS.gene049111	MS.gene29445	PPI
MS.gene29445	MS.gene60196	PPI
MS.gene29445	MS.gene29298	PPI
MS.gene70263	MS.gene29445	PPI
MS.gene58285	MS.gene29445	PPI
MS.gene29445	MS.gene46303	PPI
MS.gene29445	MS.gene28438	PPI
MS.gene29445	MS.gene64996	PPI
MS.gene29445	MS.gene26084	PPI
MS.gene29445	MS.gene066960	PPI
MS.gene29445	MS.gene78227	PPI
MS.gene29445	MS.gene063067	PPI
MS.gene29445	MS.gene066536	PPI
MS.gene067742	MS.gene29445	PPI
MS.gene78322	MS.gene29445	PPI
MS.gene011711	MS.gene29445	PPI
MS.gene87898	MS.gene29445	PPI
MS.gene29445	MS.gene03849	PPI
MS.gene61192	MS.gene29445	PPI
MS.gene29445	MS.gene35163	PPI
MS.gene28432	MS.gene29445	PPI
MS.gene29445	MS.gene49838	PPI
MS.gene88233	MS.gene29445	PPI
MS.gene82109	MS.gene29445	PPI
MS.gene06236	MS.gene29445	PPI
MS.gene95244	MS.gene29445	PPI
MS.gene055512	MS.gene29445	PPI
MS.gene94307	MS.gene29445	PPI
MS.gene29445	MS.gene81646	PPI
MS.gene29445	MS.gene66752	PPI
MS.gene78924	MS.gene29445	PPI
MS.gene25357	MS.gene29445	PPI
MS.gene07616	MS.gene29445	PPI
MS.gene98552	MS.gene29445	PPI
MS.gene29445	MS.gene70990	PPI
MS.gene29445	MS.gene24196	PPI
MS.gene29445	MS.gene068179	PPI
MS.gene29445	MS.gene027909	PPI
MS.gene29445	MS.gene70261	PPI
MS.gene066535	MS.gene29445	PPI
MS.gene027907	MS.gene29445	PPI
MS.gene28434	MS.gene29445	PPI
MS.gene29445	MS.gene072431	PPI
MS.gene014301	MS.gene29445	PPI
MS.gene29445	MS.gene64998	PPI
MS.gene000604	MS.gene29445	PPI
MS.gene29445	MS.gene78323	PPI
MS.gene58116	MS.gene29445	PPI
MS.gene29445	MS.gene91631	PPI
MS.gene29445	MS.gene58114	PPI
MS.gene29445	MS.gene04748	PPI
MS.gene030661	MS.gene29445	PPI
MS.gene29445	MS.gene50129	PPI
MS.gene79799	MS.gene29445	PPI
MS.gene29445	MS.gene60200	PPI
MS.gene97703	MS.gene29445	PPI
MS.gene29445	MS.gene32513	PPI
MS.gene059054	MS.gene29445	PPI
MS.gene29445	MS.gene05905	PPI
MS.gene29445	MS.gene024064	PPI
MS.gene29445	MS.gene64164	PPI
MS.gene29445	MS.gene41679	PPI
MS.gene29445	MS.gene36496	PPI
MS.gene60199	MS.gene29445	PPI
MS.gene29445	MS.gene057458	PPI
MS.gene021887	MS.gene29445	PPI
MS.gene29445	MS.gene29521	PPI
MS.gene009155	MS.gene29445	PPI
MS.gene52605	MS.gene29445	PPI
MS.gene020313	MS.gene29445	PPI
MS.gene29445	MS.gene26406	PPI
MS.gene070538	MS.gene29445	PPI
MS.gene44458	MS.gene29445	PPI
MS.gene39538	MS.gene29445	PPI
MS.gene29445	MS.gene072432	PPI
MS.gene042871	MS.gene29445	PPI
MS.gene78922	MS.gene29445	PPI
MS.gene071851	MS.gene29445	PPI
MS.gene29445	MS.gene018762	PPI
MS.gene016712	MS.gene29445	PPI
MS.gene29445	MS.gene00233	PPI
MS.gene030666	MS.gene29445	PPI
MS.gene29445	MS.gene034255	PPI
MS.gene29445	MS.gene030664	PPI
MS.gene066537	MS.gene29445	PPI
MS.gene97701	MS.gene29445	PPI
MS.gene29445	MS.gene47573	PPI
MS.gene29445	MS.gene94315	PPI
MS.gene29445	MS.gene66836	PPI
MS.gene29445	MS.gene47284	PPI
MS.gene29445	MS.gene55626	PPI
MS.gene027904	MS.gene29445	PPI
MS.gene29445	MS.gene61193	PPI
MS.gene014181	MS.gene29445	PPI
MS.gene29445	MS.gene069975	PPI
MS.gene29445	MS.gene34797	PPI
MS.gene29445	MS.gene78324	PPI
MS.gene068178	MS.gene29445	PPI
MS.gene44457	MS.gene29445	PPI
MS.gene013163	MS.gene29445	PPI
MS.gene29445	MS.gene017431	PPI
MS.gene29445	MS.gene70258	PPI
MS.gene32514	MS.gene29445	PPI
MS.gene29445	MS.gene65125	PPI
MS.gene68113	MS.gene29445	PPI
MS.gene053230	MS.gene29445	PPI
MS.gene064876	MS.gene29445	PPI
MS.gene29445	MS.gene056335	PPI
MS.gene29445	MS.gene58112	PPI
MS.gene29445	MS.gene011710	PPI
MS.gene29445	MS.gene29300	PPI
MS.gene66051	MS.gene29445	PPI
MS.gene028296	MS.gene29445	PPI
MS.gene29445	MS.gene23886	PPI
MS.gene04820	MS.gene29445	PPI
MS.gene29445	MS.gene29299	PPI
MS.gene29445	MS.gene015760	PPI
MS.gene072433	MS.gene29445	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29445.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene29445.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene29445.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene29445.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene29445.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene29445.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene29445.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene29445.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene29445.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene29445.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene29445.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene29445.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene29445.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene29445.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene29445.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene29445.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene29445.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene29445.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene29445.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene29445.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene29445.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene29445.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene29445.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene29445.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29445.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene29445.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene29445.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene29445.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene29445.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene29445.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene29445.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene29445.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene29445.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene29445.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene29445.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene29445.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene29445.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene29445.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene29445.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene29445.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATTTCTCTAACCAATCTT+TGG	0.234850	5.3:+64004672	None:intergenic
TCACGAAGAGCGACTGTTCC+TGG	0.301860	5.3:+64004746	None:intergenic
GCAGCTGAAGCTTATCTTGT+TGG	0.349802	5.3:-64004590	MS.gene29445:CDS
AGCTGATTTCCGAGCGGCTT+TGG	0.356003	5.3:+64004808	None:intergenic
GAAGCTTCCTTTCCAAAGAT+TGG	0.376805	5.3:-64004684	MS.gene29445:CDS
CGGAAATCAGCTCCGGCGAC+TGG	0.383526	5.3:-64004797	MS.gene29445:CDS
GAGCGGAAACAGCGCTACTC+TGG	0.433957	5.3:+64004621	None:intergenic
TTGGTGGCGAGCTGTTTCCT+TGG	0.436325	5.3:+64004827	None:intergenic
TTCTTCACTCCTCCAGTCGC+CGG	0.441465	5.3:+64004785	None:intergenic
AAGCCCTCTCGCCTCTGATT+CGG	0.461144	5.3:+64004489	None:intergenic
AGCATGAATAGCACAAAGAT+TGG	0.492949	5.3:+64004553	None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG	0.495094	5.3:+64004811	None:intergenic
CGAGCAAGTTGAATATCCTT+AGG	0.498424	5.3:+64004512	None:intergenic
AGCCGCTCGGAAATCAGCTC+CGG	0.498921	5.3:-64004804	MS.gene29445:CDS
AAACAGCGCTACTCTGGAAT+CGG	0.503171	5.3:+64004627	None:intergenic
GAGAGTTACTATTATGCCTA+AGG	0.517685	5.3:-64004528	MS.gene29445:CDS
AGAGCACAGAGCTTCTCATA+AGG	0.533669	5.3:-64004706	MS.gene29445:CDS
CAACTTGCTCGCCGAATCAG+AGG	0.534605	5.3:-64004500	MS.gene29445:CDS
TCTCTAACCAATCTTTGGAA+AGG	0.542941	5.3:+64004677	None:intergenic
AGCTCGCCACCAAAGCCGCT+CGG	0.547091	5.3:-64004817	MS.gene29445:CDS
TTCAGCTGCTTCTTGAAGAG+CGG	0.576635	5.3:+64004604	None:intergenic
TCGCCGAATCAGAGGCGAGA+GGG	0.598725	5.3:-64004492	MS.gene29445:CDS
ATTGGTTAGAGAAATCGCTC+AGG	0.600201	5.3:-64004666	MS.gene29445:CDS
CAAACAGCAAGAAAATCCAC+CGG	0.620184	5.3:-64004860	MS.gene29445:CDS
AAATCAGCTCCGGCGACTGG+AGG	0.630058	5.3:-64004794	MS.gene29445:CDS
AAGCCACACAGATTCCGTCC+AGG	0.632383	5.3:-64004764	MS.gene29445:CDS
CTCGCCGAATCAGAGGCGAG+AGG	0.633846	5.3:-64004493	MS.gene29445:CDS
GAAGAGCGACTGTTCCTGGA+CGG	0.668213	5.3:+64004750	None:intergenic
GTTCCTGGACGGAATCTGTG+TGG	0.677012	5.3:+64004761	None:intergenic
CCACCGGAGGAAAAGCACCA+AGG	0.683879	5.3:-64004844	MS.gene29445:CDS
ACAGCAAGAAAATCCACCGG+AGG	0.684797	5.3:-64004857	MS.gene29445:CDS
CGCCGGAGCTGATTTCCGAG+CGG	0.685230	5.3:+64004802	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCATGAATAGCACAAAGAT+TGG	+	chr5.3:64004812-64004831	None:intergenic	35.0%
	CGATTTCTCTAACCAATCTT+TGG	+	chr5.3:64004693-64004712	None:intergenic	35.0%
	GAGAGTTACTATTATGCCTA+AGG	-	chr5.3:64004834-64004853	MS.gene29445:CDS	35.0%
!	TCTCTAACCAATCTTTGGAA+AGG	+	chr5.3:64004688-64004707	None:intergenic	35.0%
!!!	GGATTTTCTTGCTGTTTGTT+TGG	+	chr5.3:64004500-64004519	None:intergenic	35.0%
	ATTGGTTAGAGAAATCGCTC+AGG	-	chr5.3:64004696-64004715	MS.gene29445:CDS	40.0%
	CAAACAGCAAGAAAATCCAC+CGG	-	chr5.3:64004502-64004521	MS.gene29445:CDS	40.0%
	GAAGCTTCCTTTCCAAAGAT+TGG	-	chr5.3:64004678-64004697	MS.gene29445:CDS	40.0%
!	CGAGCAAGTTGAATATCCTT+AGG	+	chr5.3:64004853-64004872	None:intergenic	40.0%
	AGAGCACAGAGCTTCTCATA+AGG	-	chr5.3:64004656-64004675	MS.gene29445:CDS	45.0%
	GCAGCTGAAGCTTATCTTGT+TGG	-	chr5.3:64004772-64004791	MS.gene29445:CDS	45.0%
	TTCAGCTGCTTCTTGAAGAG+CGG	+	chr5.3:64004761-64004780	None:intergenic	45.0%
!!	AAACAGCGCTACTCTGGAAT+CGG	+	chr5.3:64004738-64004757	None:intergenic	45.0%
!!!	TTTCCTTGGTGCTTTTCCTC+CGG	+	chr5.3:64004524-64004543	None:intergenic	45.0%
	ACAGCAAGAAAATCCACCGG+AGG	-	chr5.3:64004505-64004524	MS.gene29445:CDS	50.0%
	AAGCCACACAGATTCCGTCC+AGG	-	chr5.3:64004598-64004617	MS.gene29445:CDS	55.0%
	AAGCCCTCTCGCCTCTGATT+CGG	+	chr5.3:64004876-64004895	None:intergenic	55.0%
	GAAGAGCGACTGTTCCTGGA+CGG	+	chr5.3:64004615-64004634	None:intergenic	55.0%
	GTTCCTGGACGGAATCTGTG+TGG	+	chr5.3:64004604-64004623	None:intergenic	55.0%
	TCACGAAGAGCGACTGTTCC+TGG	+	chr5.3:64004619-64004638	None:intergenic	55.0%
	TTCTTCACTCCTCCAGTCGC+CGG	+	chr5.3:64004580-64004599	None:intergenic	55.0%
!	AGCTGATTTCCGAGCGGCTT+TGG	+	chr5.3:64004557-64004576	None:intergenic	55.0%
!	CAACTTGCTCGCCGAATCAG+AGG	-	chr5.3:64004862-64004881	MS.gene29445:CDS	55.0%
!	TGATTTCCGAGCGGCTTTGG+TGG	+	chr5.3:64004554-64004573	None:intergenic	55.0%
!!	TTGGTGGCGAGCTGTTTCCT+TGG	+	chr5.3:64004538-64004557	None:intergenic	55.0%
	AAATCAGCTCCGGCGACTGG+AGG	-	chr5.3:64004568-64004587	MS.gene29445:CDS	60.0%
	AGCCGCTCGGAAATCAGCTC+CGG	-	chr5.3:64004558-64004577	MS.gene29445:CDS	60.0%
	GAGCGGAAACAGCGCTACTC+TGG	+	chr5.3:64004744-64004763	None:intergenic	60.0%
	TCGCCGAATCAGAGGCGAGA+GGG	-	chr5.3:64004870-64004889	MS.gene29445:CDS	60.0%
!!	CCACCGGAGGAAAAGCACCA+AGG	-	chr5.3:64004518-64004537	MS.gene29445:CDS	60.0%
!!!	CCTTGGTGCTTTTCCTCCGG+TGG	+	chr5.3:64004521-64004540	None:intergenic	60.0%
	AGCTCGCCACCAAAGCCGCT+CGG	-	chr5.3:64004545-64004564	MS.gene29445:CDS	65.0%
	CGGAAATCAGCTCCGGCGAC+TGG	-	chr5.3:64004565-64004584	MS.gene29445:CDS	65.0%
	CTCGCCGAATCAGAGGCGAG+AGG	-	chr5.3:64004869-64004888	MS.gene29445:CDS	65.0%
!	CGCCGGAGCTGATTTCCGAG+CGG	+	chr5.3:64004563-64004582	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	64004487	64004897	64004487	ID=MS.gene29445
chr5.3	mRNA	64004487	64004897	64004487	ID=MS.gene29445.t1;Parent=MS.gene29445
chr5.3	exon	64004487	64004897	64004487	ID=MS.gene29445.t1.exon1;Parent=MS.gene29445.t1
chr5.3	CDS	64004487	64004897	64004487	ID=cds.MS.gene29445.t1;Parent=MS.gene29445.t1

Gene Sequence

>MS.gene29445

ATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCACCAAGGAAACAGCTCGCCACCAAAGCCGCTCGGAAATCAGCTCCGGCGACTGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTCGCTCTTCGTGAAATCAGAAAGTATCAGAAGAGCACAGAGCTTCTCATAAGGAAGCTTCCTTTCCAAAGATTGGTTAGAGAAATCGCTCAGGATTTCAAAACTGATCTCCGATTCCAGAGTAGCGCTGTTTCCGCTCTTCAAGAAGCAGCTGAAGCTTATCTTGTTGGCTTGTTTGAAGATACCAATCTTTGTGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCTCGCCGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MS.gene29445.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA