AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66051


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66051.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene66051.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene66051.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene66051.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene66051.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene66051.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene66051.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene66051.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene66051.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene66051.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene66051.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene66051.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene66051.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene66051.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene66051.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene66051.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene66051.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene66051.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene66051.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene66051.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene66051.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene66051.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene66051.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene66051.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66051.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene66051.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene66051.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene66051.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene66051.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene66051.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene66051.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene66051.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene66051.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene66051.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene66051.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene66051.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene66051.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene66051.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene66051.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene66051.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACGAAGCGCGACAGTTCC+AGG 0.362532 7.3:+45764339 None:intergenic
GCTGCTGAAGCGTATCTTGT+TGG 0.411498 7.3:-45764183 MS.gene66051:CDS
GGATTTGCGTGCTGTTTGTT+TGG 0.426439 7.3:+45764458 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 7.3:+45764378 None:intergenic
CTTGCAAGTTGAATATCCTT+AGG 0.450286 7.3:+45764105 None:intergenic
CGAAAATCAGCTCCGGCGAC+CGG 0.463917 7.3:-45764390 MS.gene66051:CDS
TGGTGGCGAGTTGTTTGCGT+GGG 0.485319 7.3:+45764421 None:intergenic
CAAGGAGAATTCGTGGTGAA+AGG 0.485929 7.3:-45764086 MS.gene66051:CDS
AAGCCACACCGTTTCCGTCC+TGG 0.490087 7.3:-45764357 MS.gene66051:CDS
CGACAGTTCCAGGACGGAAA+CGG 0.513929 7.3:+45764349 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 7.3:-45764121 MS.gene66051:CDS
AAGGAGAATTCGTGGTGAAA+GGG 0.519150 7.3:-45764085 MS.gene66051:CDS
AGAGCACTGAGCTTCTCATA+AGG 0.520360 7.3:-45764299 MS.gene66051:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.526132 7.3:+45764371 None:intergenic
AGCCGCTCGAAAATCAGCTC+CGG 0.532014 7.3:-45764397 MS.gene66051:CDS
CAACTTGCAAGGAGAATTCG+TGG 0.532409 7.3:-45764093 MS.gene66051:CDS
CAAACAGCACGCAAATCCAC+CGG 0.549355 7.3:-45764453 MS.gene66051:CDS
CAGTGCTCTTCTGATACTTG+CGG 0.560252 7.3:+45764313 None:intergenic
ACTGCTTTGAAATCTGAGAT+CGG 0.560988 7.3:+45764227 None:intergenic
CTAAGGATATTCAACTTGCA+AGG 0.586821 7.3:-45764104 MS.gene66051:CDS
TCTTGTTCGTGAAATCGCAC+AGG 0.597491 7.3:-45764259 MS.gene66051:CDS
TTGGTGGCGAGTTGTTTGCG+TGG 0.598004 7.3:+45764420 None:intergenic
TCACGAACAAGACGTTGGAA+TGG 0.598115 7.3:+45764270 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 7.3:-45764387 MS.gene66051:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 7.3:+45764265 None:intergenic
GTTCCAGGACGGAAACGGTG+TGG 0.690959 7.3:+45764354 None:intergenic
ACAGCACGCAAATCCACCGG+CGG 0.691035 7.3:-45764450 MS.gene66051:CDS
GGTGGCGAGTTGTTTGCGTG+GGG 0.692652 7.3:+45764422 None:intergenic
GAAGCGCGACAGTTCCAGGA+CGG 0.694526 7.3:+45764343 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTAAGGATATTCAACTTGCA+AGG - chr7.3:45764444-45764463 MS.gene66051:CDS 35.0%
GAGAGTTACTATTATGCCTA+AGG - chr7.3:45764427-45764446 MS.gene66051:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG + chr7.3:45764446-45764465 None:intergenic 35.0%
!! ACTGCTTTGAAATCTGAGAT+CGG + chr7.3:45764324-45764343 None:intergenic 35.0%
AAGGAGAATTCGTGGTGAAA+GGG - chr7.3:45764463-45764482 MS.gene66051:CDS 40.0%
CAACTTGCAAGGAGAATTCG+TGG - chr7.3:45764455-45764474 MS.gene66051:CDS 45.0%
CAAGGAGAATTCGTGGTGAA+AGG - chr7.3:45764462-45764481 MS.gene66051:CDS 45.0%
CAGTGCTCTTCTGATACTTG+CGG + chr7.3:45764238-45764257 None:intergenic 45.0%
CGATTTCACGAACAAGACGT+TGG + chr7.3:45764286-45764305 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr7.3:45764281-45764300 None:intergenic 45.0%
TCTTGTTCGTGAAATCGCAC+AGG - chr7.3:45764289-45764308 MS.gene66051:CDS 45.0%
!! AGAGCACTGAGCTTCTCATA+AGG - chr7.3:45764249-45764268 MS.gene66051:CDS 45.0%
!! GGATTTGCGTGCTGTTTGTT+TGG + chr7.3:45764093-45764112 None:intergenic 45.0%
CAAACAGCACGCAAATCCAC+CGG - chr7.3:45764095-45764114 MS.gene66051:CDS 50.0%
GCTGCTGAAGCGTATCTTGT+TGG - chr7.3:45764365-45764384 MS.gene66051:CDS 50.0%
!! AGCTGATTTTCGAGCGGCTT+TGG + chr7.3:45764150-45764169 None:intergenic 50.0%
!! TGATTTTCGAGCGGCTTTGG+TGG + chr7.3:45764147-45764166 None:intergenic 50.0%
AGCCGCTCGAAAATCAGCTC+CGG - chr7.3:45764151-45764170 MS.gene66051:CDS 55.0%
CGACAGTTCCAGGACGGAAA+CGG + chr7.3:45764202-45764221 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr7.3:45764180-45764199 None:intergenic 55.0%
! TGGTGGCGAGTTGTTTGCGT+GGG + chr7.3:45764130-45764149 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTGCG+TGG + chr7.3:45764131-45764150 None:intergenic 55.0%
AAGCCACACCGTTTCCGTCC+TGG - chr7.3:45764191-45764210 MS.gene66051:CDS 60.0%
ACAGCACGCAAATCCACCGG+CGG - chr7.3:45764098-45764117 MS.gene66051:CDS 60.0%
CGAAAATCAGCTCCGGCGAC+CGG - chr7.3:45764158-45764177 MS.gene66051:CDS 60.0%
GAAGCGCGACAGTTCCAGGA+CGG + chr7.3:45764208-45764227 None:intergenic 60.0%
GGTGGCGAGTTGTTTGCGTG+GGG + chr7.3:45764129-45764148 None:intergenic 60.0%
GTTCCAGGACGGAAACGGTG+TGG + chr7.3:45764197-45764216 None:intergenic 60.0%
TCACGAAGCGCGACAGTTCC+AGG + chr7.3:45764212-45764231 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr7.3:45764173-45764192 None:intergenic 60.0%
! TTTGCGTGGGGCTTTTCCGC+CGG + chr7.3:45764117-45764136 None:intergenic 60.0%
!! CGCCGGAGCTGATTTTCGAG+CGG + chr7.3:45764156-45764175 None:intergenic 60.0%
AAATCAGCTCCGGCGACCGG+AGG - chr7.3:45764161-45764180 MS.gene66051:CDS 65.0%
! GCGTGGGGCTTTTCCGCCGG+TGG + chr7.3:45764114-45764133 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 45764080 45764490 45764080 ID=MS.gene66051
chr7.3 mRNA 45764080 45764490 45764080 ID=MS.gene66051.t1;Parent=MS.gene66051
chr7.3 exon 45764080 45764490 45764080 ID=MS.gene66051.t1.exon1;Parent=MS.gene66051.t1
chr7.3 CDS 45764080 45764490 45764080 ID=cds.MS.gene66051.t1;Parent=MS.gene66051.t1
Gene Sequence

>MS.gene66051

ATGGCACGAACCAAACAAACAGCACGCAAATCCACCGGCGGAAAAGCCCCACGCAAACAACTCGCCACCAAAGCCGCTCGAAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACCGTTTCCGTCCTGGAACTGTCGCGCTTCGTGAAATCCGCAAGTATCAGAAGAGCACTGAGCTTCTCATAAGGAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCACAGGATTTCAAAACCGATCTCAGATTTCAAAGCAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCGTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGCGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGGAGAATTCGTGGTGAAAGGGCTTAG

Protein sequence

>MS.gene66051.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA