AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78224


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78224.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene78224.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene78224.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene78224.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene78224.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene78224.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene78224.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene78224.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene78224.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene78224.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene78224.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene78224.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene78224.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene78224.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene78224.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene78224.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene78224.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene78224.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene78224.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene78224.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene78224.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene78224.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene78224.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene78224.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78224.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene78224.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene78224.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene78224.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene78224.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene78224.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene78224.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene78224.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene78224.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene78224.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene78224.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene78224.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene78224.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene78224.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene78224.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene78224.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAGCTCTGTGCTCTTC+TGG 0.285934 7.4:+44409969 None:intergenic
AGCTGATTTCCGTGCGGCTT+TGG 0.327301 7.4:+44410066 None:intergenic
TTCACGAAGCGCGACGGTTC+CGG 0.341493 7.4:+44410003 None:intergenic
TCACGAAGCGCGACGGTTCC+GGG 0.353522 7.4:+44410004 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 7.4:-44409848 MS.gene78224:CDS
CTTGCAAGTTGAATATCCTT+AGG 0.413826 7.4:+44409770 None:intergenic
GAGCAGAAACAGCACTGCTC+TGG 0.422418 7.4:+44409879 None:intergenic
GGATTTGCGTGCTGTTTGTT+TGG 0.426439 7.4:+44410123 None:intergenic
CAAGAAGAATTCGCGGTGAA+AGG 0.426749 7.4:-44409751 MS.gene78224:CDS
CTGTGCTCTTCTGGTACTTA+CGG 0.435668 7.4:+44409978 None:intergenic
TGATTTCCGTGCGGCTTTGG+TGG 0.441149 7.4:+44410069 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 7.4:+44410043 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.469854 7.4:-44410055 MS.gene78224:CDS
TGGTGGCGAGTTGTTTGCGT+GGG 0.485319 7.4:+44410086 None:intergenic
AAGAAGAATTCGCGGTGAAA+GGG 0.496217 7.4:-44409750 MS.gene78224:CDS
AGCCGCACGGAAATCAGCTC+CGG 0.497667 7.4:-44410062 MS.gene78224:CDS
AGAGCACAGAGCTTCTCATA+AGG 0.512945 7.4:-44409964 MS.gene78224:CDS
CGACGGTTCCGGGACGGAAA+CGG 0.513929 7.4:+44410014 None:intergenic
GAGAGTTACTATTATGCCTA+AGG 0.517685 7.4:-44409786 MS.gene78224:CDS
CAACTTGCAAGAAGAATTCG+CGG 0.524653 7.4:-44409758 MS.gene78224:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.526132 7.4:+44410036 None:intergenic
AAGCCACACCGTTTCCGTCC+CGG 0.544193 7.4:-44410022 MS.gene78224:CDS
CAAACAGCACGCAAATCCAC+CGG 0.549355 7.4:-44410118 MS.gene78224:CDS
TCTTGTTCGTGAAATCGCTC+AGG 0.549771 7.4:-44409924 MS.gene78224:CDS
TTGGTGGCGAGTTGTTTGCG+TGG 0.598004 7.4:+44410085 None:intergenic
TCACGAACAAGACGTTGGAA+TGG 0.598115 7.4:+44409935 None:intergenic
ACTGCTCTGGAATCTGAGAT+CGG 0.602457 7.4:+44409892 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.606043 7.4:-44410052 MS.gene78224:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 7.4:+44409930 None:intergenic
ACGGATTTCACGAAGCGCGA+CGG 0.624291 7.4:+44409997 None:intergenic
AACTCGCCACCAAAGCCGCA+CGG 0.635817 7.4:-44410075 MS.gene78224:CDS
GAAGCGCGACGGTTCCGGGA+CGG 0.640203 7.4:+44410008 None:intergenic
GTTCCGGGACGGAAACGGTG+TGG 0.652674 7.4:+44410019 None:intergenic
ACAGCACGCAAATCCACCGG+CGG 0.691035 7.4:-44410115 MS.gene78224:CDS
GGTGGCGAGTTGTTTGCGTG+GGG 0.692652 7.4:+44410087 None:intergenic
CGCCGGAGCTGATTTCCGTG+CGG 0.701307 7.4:+44410060 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAGAGTTACTATTATGCCTA+AGG - chr7.4:44410092-44410111 MS.gene78224:CDS 35.0%
! CTTGCAAGTTGAATATCCTT+AGG + chr7.4:44410111-44410130 None:intergenic 35.0%
AAGAAGAATTCGCGGTGAAA+GGG - chr7.4:44410128-44410147 MS.gene78224:CDS 40.0%
CAACTTGCAAGAAGAATTCG+CGG - chr7.4:44410120-44410139 MS.gene78224:CDS 40.0%
AGAGCACAGAGCTTCTCATA+AGG - chr7.4:44409914-44409933 MS.gene78224:CDS 45.0%
CAAGAAGAATTCGCGGTGAA+AGG - chr7.4:44410127-44410146 MS.gene78224:CDS 45.0%
CGATTTCACGAACAAGACGT+TGG + chr7.4:44409951-44409970 None:intergenic 45.0%
CTGTGCTCTTCTGGTACTTA+CGG + chr7.4:44409903-44409922 None:intergenic 45.0%
GCTGCTGAAGCTTATCTTGT+TGG - chr7.4:44410030-44410049 MS.gene78224:CDS 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr7.4:44409946-44409965 None:intergenic 45.0%
TCTTGTTCGTGAAATCGCTC+AGG - chr7.4:44409954-44409973 MS.gene78224:CDS 45.0%
!! ACTGCTCTGGAATCTGAGAT+CGG + chr7.4:44409989-44410008 None:intergenic 45.0%
!! GGATTTGCGTGCTGTTTGTT+TGG + chr7.4:44409758-44409777 None:intergenic 45.0%
CAAACAGCACGCAAATCCAC+CGG - chr7.4:44409760-44409779 MS.gene78224:CDS 50.0%
TGAGAAGCTCTGTGCTCTTC+TGG + chr7.4:44409912-44409931 None:intergenic 50.0%
ACGGATTTCACGAAGCGCGA+CGG + chr7.4:44409884-44409903 None:intergenic 55.0%
GAGCAGAAACAGCACTGCTC+TGG + chr7.4:44410002-44410021 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr7.4:44409845-44409864 None:intergenic 55.0%
! AGCTGATTTCCGTGCGGCTT+TGG + chr7.4:44409815-44409834 None:intergenic 55.0%
! TGATTTCCGTGCGGCTTTGG+TGG + chr7.4:44409812-44409831 None:intergenic 55.0%
! TGGTGGCGAGTTGTTTGCGT+GGG + chr7.4:44409795-44409814 None:intergenic 55.0%
!! TTGGTGGCGAGTTGTTTGCG+TGG + chr7.4:44409796-44409815 None:intergenic 55.0%
AACTCGCCACCAAAGCCGCA+CGG - chr7.4:44409803-44409822 MS.gene78224:CDS 60.0%
AAGCCACACCGTTTCCGTCC+CGG - chr7.4:44409856-44409875 MS.gene78224:CDS 60.0%
ACAGCACGCAAATCCACCGG+CGG - chr7.4:44409763-44409782 MS.gene78224:CDS 60.0%
AGCCGCACGGAAATCAGCTC+CGG - chr7.4:44409816-44409835 MS.gene78224:CDS 60.0%
GGTGGCGAGTTGTTTGCGTG+GGG + chr7.4:44409794-44409813 None:intergenic 60.0%
TTCACGAAGCGCGACGGTTC+CGG + chr7.4:44409878-44409897 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr7.4:44409838-44409857 None:intergenic 60.0%
! TTTGCGTGGGGCTTTTCCGC+CGG + chr7.4:44409782-44409801 None:intergenic 60.0%
AAATCAGCTCCGGCGACCGG+AGG - chr7.4:44409826-44409845 MS.gene78224:CDS 65.0%
CGACGGTTCCGGGACGGAAA+CGG + chr7.4:44409867-44409886 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+CGG - chr7.4:44409823-44409842 MS.gene78224:CDS 65.0%
GTTCCGGGACGGAAACGGTG+TGG + chr7.4:44409862-44409881 None:intergenic 65.0%
TCACGAAGCGCGACGGTTCC+GGG + chr7.4:44409877-44409896 None:intergenic 65.0%
! CGCCGGAGCTGATTTCCGTG+CGG + chr7.4:44409821-44409840 None:intergenic 65.0%
GAAGCGCGACGGTTCCGGGA+CGG + chr7.4:44409873-44409892 None:intergenic 70.0%
! GCGTGGGGCTTTTCCGCCGG+TGG + chr7.4:44409779-44409798 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 44409745 44410155 44409745 ID=MS.gene78224
chr7.4 mRNA 44409745 44410155 44409745 ID=MS.gene78224.t1;Parent=MS.gene78224
chr7.4 exon 44409745 44410155 44409745 ID=MS.gene78224.t1.exon1;Parent=MS.gene78224.t1
chr7.4 CDS 44409745 44410155 44409745 ID=cds.MS.gene78224.t1;Parent=MS.gene78224.t1
Gene Sequence

>MS.gene78224

ATGGCACGAACCAAACAAACAGCACGCAAATCCACCGGCGGAAAAGCCCCACGCAAACAACTCGCCACCAAAGCCGCACGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACCGTTTCCGTCCCGGAACCGTCGCGCTTCGTGAAATCCGTAAGTACCAGAAGAGCACAGAGCTTCTCATAAGGAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAGGATTTCAAAACCGATCTCAGATTCCAGAGCAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGCGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCAAGAAGAATTCGCGGTGAAAGGGCCTAG

Protein sequence

>MS.gene78224.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA