AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009155


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009155.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene009155.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene009155.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene009155.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene009155.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene009155.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene009155.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene009155.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene009155.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene009155.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene009155.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene009155.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene009155.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene009155.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene009155.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene009155.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene009155.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene009155.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene009155.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene009155.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene009155.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene009155.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene009155.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene009155.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009155.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene009155.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene009155.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene009155.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene009155.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene009155.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene009155.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene009155.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene009155.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene009155.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene009155.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene009155.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene009155.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene009155.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene009155.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene009155.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAGCCACACCGTTTC+AGG 0.224781 8.1:-39945516 MS.gene009155:CDS
TCACGGAGTGCGACGGTTCC+TGG 0.249130 8.1:+39945493 None:intergenic
TGAGTAGTTCGGTGCTCTTC+TGG 0.344215 8.1:+39945458 None:intergenic
GCGGCGGAGGCTTATCTTGT+TGG 0.386513 8.1:-39945337 MS.gene009155:CDS
CTTCCTTGGTGCTTTGCCGC+CGG 0.391014 8.1:+39945588 None:intergenic
TTTGTTGCGAGTTGCTTCCT+TGG 0.401679 8.1:+39945574 None:intergenic
TTCTTCACTCCGCCGGTCGC+CGG 0.410833 8.1:+39945532 None:intergenic
CGGAAATCCGCTCCGGCGAC+CGG 0.410851 8.1:-39945544 MS.gene009155:CDS
CCTTGGTGCTTTGCCGCCGG+TGG 0.443251 8.1:+39945591 None:intergenic
TTATCTTGTTGGATTGTTTG+AGG 0.444671 8.1:-39945326 MS.gene009155:CDS
GGCGCTGCAAGAAGCGGCGG+AGG 0.459160 8.1:-39945350 MS.gene009155:CDS
CGACGGTTCCTGGCCTGAAA+CGG 0.480580 8.1:+39945503 None:intergenic
GACGCTGGAAAGGAAGCTTC+CGG 0.481198 8.1:+39945434 None:intergenic
CTAGCAAGCTGAATATCCTT+AGG 0.499598 8.1:+39945259 None:intergenic
AAGCCACACCGTTTCAGGCC+AGG 0.512079 8.1:-39945511 MS.gene009155:CDS
CTAAGGATATTCAGCTTGCT+AGG 0.512436 8.1:-39945258 MS.gene009155:CDS
AGCCGCTCGGAAATCCGCTC+CGG 0.515753 8.1:-39945551 MS.gene009155:CDS
AAGCTTCCGGATGAGTAGTT+CGG 0.529555 8.1:+39945447 None:intergenic
ACACAGCAGAGCTCTGAAAA+CGG 0.532576 8.1:+39945374 None:intergenic
CACTCCGCCGGTCGCCGGAG+CGG 0.534210 8.1:+39945537 None:intergenic
GTGTGGCTTCTTCACTCCGC+CGG 0.542673 8.1:+39945525 None:intergenic
CGGTGCTCTTCTGGTACTTG+CGG 0.546004 8.1:+39945467 None:intergenic
GCGTGTTACTATCATGCCTA+AGG 0.550012 8.1:-39945275 MS.gene009155:CDS
TCTGGTACTTGCGGATCTCA+CGG 0.559013 8.1:+39945476 None:intergenic
AGAGCACCGAACTACTCATC+CGG 0.565108 8.1:-39945453 MS.gene009155:CDS
AAATCCGCTCCGGCGACCGG+CGG 0.570605 8.1:-39945541 MS.gene009155:CDS
TCTTGTTCGTGAGATCGCTC+AGG 0.579084 8.1:-39945413 MS.gene009155:CDS
TCACGAACAAGACGCTGGAA+AGG 0.582899 8.1:+39945424 None:intergenic
AACTCGCAACAAAAGCCGCT+CGG 0.592966 8.1:-39945564 MS.gene009155:CDS
CGATCTCACGAACAAGACGC+TGG 0.602860 8.1:+39945419 None:intergenic
TGTGTCGGCGCTGCAAGAAG+CGG 0.603200 8.1:-39945356 MS.gene009155:CDS
GTCGGCGCTGCAAGAAGCGG+CGG 0.613578 8.1:-39945353 MS.gene009155:CDS
ACCGCACGAAAATCCACCGG+CGG 0.621620 8.1:-39945604 MS.gene009155:CDS
AAGATCAAGCACGCTCGCCA+CGG 0.626947 8.1:+39945230 None:intergenic
CCACCGGCGGCAAAGCACCA+AGG 0.632902 8.1:-39945591 MS.gene009155:CDS
GCGGATCTCACGGAGTGCGA+CGG 0.634415 8.1:+39945486 None:intergenic
GTTCCTGGCCTGAAACGGTG+TGG 0.654320 8.1:+39945508 None:intergenic
CAGCTTGCTAGGCGTATCCG+TGG 0.666195 8.1:-39945247 MS.gene009155:CDS
CGCCGGAGCGGATTTCCGAG+CGG 0.670325 8.1:+39945549 None:intergenic
GATCGCTCAGGATTTCAAGA+CGG 0.679659 8.1:-39945401 MS.gene009155:CDS
CAAACCGCACGAAAATCCAC+CGG 0.685560 8.1:-39945607 MS.gene009155:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTATCTTGTTGGATTGTTTG+AGG - chr8.1:39945530-39945549 MS.gene009155:CDS 30.0%
CTAGCAAGCTGAATATCCTT+AGG + chr8.1:39945600-39945619 None:intergenic 40.0%
! CTAAGGATATTCAGCTTGCT+AGG - chr8.1:39945598-39945617 MS.gene009155:CDS 40.0%
AAGCTTCCGGATGAGTAGTT+CGG + chr8.1:39945412-39945431 None:intergenic 45.0%
GATCGCTCAGGATTTCAAGA+CGG - chr8.1:39945455-39945474 MS.gene009155:CDS 45.0%
TTTGTTGCGAGTTGCTTCCT+TGG + chr8.1:39945285-39945304 None:intergenic 45.0%
! GCGTGTTACTATCATGCCTA+AGG - chr8.1:39945581-39945600 MS.gene009155:CDS 45.0%
! TTTTCAGAGCTCTGCTGTGT+CGG - chr8.1:39945485-39945504 MS.gene009155:CDS 45.0%
!! ACACAGCAGAGCTCTGAAAA+CGG + chr8.1:39945485-39945504 None:intergenic 45.0%
AACTCGCAACAAAAGCCGCT+CGG - chr8.1:39945292-39945311 MS.gene009155:CDS 50.0%
CAAACCGCACGAAAATCCAC+CGG - chr8.1:39945249-39945268 MS.gene009155:CDS 50.0%
TCACGAACAAGACGCTGGAA+AGG + chr8.1:39945435-39945454 None:intergenic 50.0%
TCTGGTACTTGCGGATCTCA+CGG + chr8.1:39945383-39945402 None:intergenic 50.0%
TCTTGTTCGTGAGATCGCTC+AGG - chr8.1:39945443-39945462 MS.gene009155:CDS 50.0%
TGAAGAAGCCACACCGTTTC+AGG - chr8.1:39945340-39945359 MS.gene009155:CDS 50.0%
!! AGAGCACCGAACTACTCATC+CGG - chr8.1:39945403-39945422 MS.gene009155:CDS 50.0%
!! TGAGTAGTTCGGTGCTCTTC+TGG + chr8.1:39945401-39945420 None:intergenic 50.0%
CGATCTCACGAACAAGACGC+TGG + chr8.1:39945440-39945459 None:intergenic 55.0%
CGGTGCTCTTCTGGTACTTG+CGG + chr8.1:39945392-39945411 None:intergenic 55.0%
GACGCTGGAAAGGAAGCTTC+CGG + chr8.1:39945425-39945444 None:intergenic 55.0%
AAGCCACACCGTTTCAGGCC+AGG - chr8.1:39945345-39945364 MS.gene009155:CDS 60.0%
ACCGCACGAAAATCCACCGG+CGG - chr8.1:39945252-39945271 MS.gene009155:CDS 60.0%
CGACGGTTCCTGGCCTGAAA+CGG + chr8.1:39945356-39945375 None:intergenic 60.0%
GCGGCGGAGGCTTATCTTGT+TGG - chr8.1:39945519-39945538 MS.gene009155:CDS 60.0%
GTGTGGCTTCTTCACTCCGC+CGG + chr8.1:39945334-39945353 None:intergenic 60.0%
GTTCCTGGCCTGAAACGGTG+TGG + chr8.1:39945351-39945370 None:intergenic 60.0%
! TGTGTCGGCGCTGCAAGAAG+CGG - chr8.1:39945500-39945519 MS.gene009155:CDS 60.0%
!! CAGCTTGCTAGGCGTATCCG+TGG - chr8.1:39945609-39945628 MS.gene009155:CDS 60.0%
!! CTTCCTTGGTGCTTTGCCGC+CGG + chr8.1:39945271-39945290 None:intergenic 60.0%
AGCCGCTCGGAAATCCGCTC+CGG - chr8.1:39945305-39945324 MS.gene009155:CDS 65.0%
GCGGATCTCACGGAGTGCGA+CGG + chr8.1:39945373-39945392 None:intergenic 65.0%
TCACGGAGTGCGACGGTTCC+TGG + chr8.1:39945366-39945385 None:intergenic 65.0%
TTCTTCACTCCGCCGGTCGC+CGG + chr8.1:39945327-39945346 None:intergenic 65.0%
!! GCCGCCGGTGGATTTTCGTG+CGG + chr8.1:39945256-39945275 None:intergenic 65.0%
AAATCCGCTCCGGCGACCGG+CGG - chr8.1:39945315-39945334 MS.gene009155:CDS 70.0%
CGGAAATCCGCTCCGGCGAC+CGG - chr8.1:39945312-39945331 MS.gene009155:CDS 70.0%
GTCGGCGCTGCAAGAAGCGG+CGG - chr8.1:39945503-39945522 MS.gene009155:CDS 70.0%
! CCACCGGCGGCAAAGCACCA+AGG - chr8.1:39945265-39945284 MS.gene009155:CDS 70.0%
! CGCCGGAGCGGATTTCCGAG+CGG + chr8.1:39945310-39945329 None:intergenic 70.0%
!! CCTTGGTGCTTTGCCGCCGG+TGG + chr8.1:39945268-39945287 None:intergenic 70.0%
GGCGCTGCAAGAAGCGGCGG+AGG - chr8.1:39945506-39945525 MS.gene009155:CDS 75.0%
! CACTCCGCCGGTCGCCGGAG+CGG + chr8.1:39945322-39945341 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 39945234 39945644 39945234 ID=MS.gene009155
chr8.1 mRNA 39945234 39945644 39945234 ID=MS.gene009155.t1;Parent=MS.gene009155
chr8.1 exon 39945234 39945644 39945234 ID=MS.gene009155.t1.exon1;Parent=MS.gene009155.t1
chr8.1 CDS 39945234 39945644 39945234 ID=cds.MS.gene009155.t1;Parent=MS.gene009155.t1
Gene Sequence

>MS.gene009155

ATGGCACGTACTAAGCAAACCGCACGAAAATCCACCGGCGGCAAAGCACCAAGGAAGCAACTCGCAACAAAAGCCGCTCGGAAATCCGCTCCGGCGACCGGCGGAGTGAAGAAGCCACACCGTTTCAGGCCAGGAACCGTCGCACTCCGTGAGATCCGCAAGTACCAGAAGAGCACCGAACTACTCATCCGGAAGCTTCCTTTCCAGCGTCTTGTTCGTGAGATCGCTCAGGATTTCAAGACGGATCTCCGTTTTCAGAGCTCTGCTGTGTCGGCGCTGCAAGAAGCGGCGGAGGCTTATCTTGTTGGATTGTTTGAGGATACTAATCTTTGCGCTATTCATGCTAAGCGTGTTACTATCATGCCTAAGGATATTCAGCTTGCTAGGCGTATCCGTGGCGAGCGTGCTTGA

Protein sequence

>MS.gene009155.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA