Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009155.t1 | XP_004512510.2 | 100 | 136 | 0 | 0 | 1 | 136 | 104 | 239 | 5.80E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009155.t1 | P68429 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 1.4e-66 | 253.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009155.t1 | A0A1S3TJX1 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.2e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009155.t1 | MTR_7g059070 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_5g029820 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_5g029770 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_4g065990 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_4g088150 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_2g035230 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_8g092720 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_8g063500 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_8g103245 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_8g092820 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 4.79e-96 | 272 |
| MS.gene009155.t1 | MTR_7g013610 | 100.000 | 136 | 0 | 0 | 1 | 136 | 100 | 235 | 1.10e-95 | 275 |
| MS.gene009155.t1 | MTR_4g097175 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene009155.t1 | MTR_1g023630 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene009155.t1 | MTR_8g061940 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.29e-93 | 265 |
| MS.gene009155.t1 | MTR_8g099430 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.38e-92 | 264 |
| MS.gene009155.t1 | MTR_4g097170 | 96.324 | 136 | 5 | 0 | 1 | 136 | 42 | 177 | 2.29e-91 | 262 |
| MS.gene009155.t1 | MTR_2g082310 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.38e-86 | 248 |
| MS.gene009155.t1 | MTR_2g082340 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.38e-86 | 248 |
| MS.gene009155.t1 | MTR_2g082370 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.74e-82 | 238 |
| MS.gene009155.t1 | MTR_2g082400 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 6.38e-75 | 219 |
| MS.gene009155.t1 | MTR_7g013600 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 1.01e-65 | 195 |
| MS.gene009155.t1 | MTR_7g023830 | 67.164 | 134 | 43 | 1 | 1 | 134 | 1 | 133 | 6.48e-56 | 171 |
| MS.gene009155.t1 | MTR_1g023630 | 73.504 | 117 | 15 | 3 | 1 | 111 | 1 | 107 | 3.81e-52 | 160 |
| MS.gene009155.t1 | MTR_5g024630 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.63e-45 | 144 |
| MS.gene009155.t1 | MTR_7g023880 | 58.647 | 133 | 41 | 3 | 3 | 134 | 18 | 137 | 5.46e-43 | 139 |
| MS.gene009155.t1 | MTR_5g024530 | 68.182 | 110 | 12 | 2 | 19 | 128 | 68 | 154 | 4.56e-42 | 137 |
| MS.gene009155.t1 | MTR_0197s0100 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 1.45e-40 | 132 |
| MS.gene009155.t1 | MTR_8g027840 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 4.83e-30 | 105 |
| MS.gene009155.t1 | MTR_8g105150 | 53.846 | 91 | 10 | 1 | 37 | 127 | 11 | 69 | 1.43e-25 | 92.4 |
| MS.gene009155.t1 | MTR_8g092740 | 97.368 | 38 | 1 | 0 | 99 | 136 | 118 | 155 | 4.60e-20 | 80.9 |
| MS.gene009155.t1 | MTR_5g089120 | 76.190 | 42 | 10 | 0 | 82 | 123 | 6 | 47 | 6.07e-18 | 73.6 |
| MS.gene009155.t1 | MTR_5g024600 | 62.963 | 54 | 20 | 0 | 1 | 54 | 1 | 54 | 1.76e-15 | 66.2 |
| MS.gene009155.t1 | MTR_4g128040 | 75.676 | 37 | 9 | 0 | 79 | 115 | 20 | 56 | 3.38e-15 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009155.t1 | AT5G65360 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene009155.t1 | AT5G10390 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene009155.t1 | AT5G10400 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene009155.t1 | AT3G27360 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene009155.t1 | AT1G09200 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.55e-95 | 271 |
| MS.gene009155.t1 | AT5G10980 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40040 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.44e-93 | 265 |
| MS.gene009155.t1 | AT4G40030 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 6.76e-93 | 266 |
| MS.gene009155.t1 | AT5G65350 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.09e-91 | 260 |
| MS.gene009155.t1 | AT1G75600 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.20e-89 | 256 |
| MS.gene009155.t1 | AT1G13370 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.32e-89 | 254 |
| MS.gene009155.t1 | AT1G19890 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.03e-83 | 241 |
| MS.gene009155.t1 | AT5G12910 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.51e-67 | 198 |
| MS.gene009155.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene009155.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene009155.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
| MS.gene009155.t1 | AT1G01370 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.86e-31 | 110 |
Find 41 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAAGCCACACCGTTTC+AGG | 0.224781 | 8.1:-39945516 | MS.gene009155:CDS |
| TCACGGAGTGCGACGGTTCC+TGG | 0.249130 | 8.1:+39945493 | None:intergenic |
| TGAGTAGTTCGGTGCTCTTC+TGG | 0.344215 | 8.1:+39945458 | None:intergenic |
| GCGGCGGAGGCTTATCTTGT+TGG | 0.386513 | 8.1:-39945337 | MS.gene009155:CDS |
| CTTCCTTGGTGCTTTGCCGC+CGG | 0.391014 | 8.1:+39945588 | None:intergenic |
| TTTGTTGCGAGTTGCTTCCT+TGG | 0.401679 | 8.1:+39945574 | None:intergenic |
| TTCTTCACTCCGCCGGTCGC+CGG | 0.410833 | 8.1:+39945532 | None:intergenic |
| CGGAAATCCGCTCCGGCGAC+CGG | 0.410851 | 8.1:-39945544 | MS.gene009155:CDS |
| CCTTGGTGCTTTGCCGCCGG+TGG | 0.443251 | 8.1:+39945591 | None:intergenic |
| TTATCTTGTTGGATTGTTTG+AGG | 0.444671 | 8.1:-39945326 | MS.gene009155:CDS |
| GGCGCTGCAAGAAGCGGCGG+AGG | 0.459160 | 8.1:-39945350 | MS.gene009155:CDS |
| CGACGGTTCCTGGCCTGAAA+CGG | 0.480580 | 8.1:+39945503 | None:intergenic |
| GACGCTGGAAAGGAAGCTTC+CGG | 0.481198 | 8.1:+39945434 | None:intergenic |
| CTAGCAAGCTGAATATCCTT+AGG | 0.499598 | 8.1:+39945259 | None:intergenic |
| AAGCCACACCGTTTCAGGCC+AGG | 0.512079 | 8.1:-39945511 | MS.gene009155:CDS |
| CTAAGGATATTCAGCTTGCT+AGG | 0.512436 | 8.1:-39945258 | MS.gene009155:CDS |
| AGCCGCTCGGAAATCCGCTC+CGG | 0.515753 | 8.1:-39945551 | MS.gene009155:CDS |
| AAGCTTCCGGATGAGTAGTT+CGG | 0.529555 | 8.1:+39945447 | None:intergenic |
| ACACAGCAGAGCTCTGAAAA+CGG | 0.532576 | 8.1:+39945374 | None:intergenic |
| CACTCCGCCGGTCGCCGGAG+CGG | 0.534210 | 8.1:+39945537 | None:intergenic |
| GTGTGGCTTCTTCACTCCGC+CGG | 0.542673 | 8.1:+39945525 | None:intergenic |
| CGGTGCTCTTCTGGTACTTG+CGG | 0.546004 | 8.1:+39945467 | None:intergenic |
| GCGTGTTACTATCATGCCTA+AGG | 0.550012 | 8.1:-39945275 | MS.gene009155:CDS |
| TCTGGTACTTGCGGATCTCA+CGG | 0.559013 | 8.1:+39945476 | None:intergenic |
| AGAGCACCGAACTACTCATC+CGG | 0.565108 | 8.1:-39945453 | MS.gene009155:CDS |
| AAATCCGCTCCGGCGACCGG+CGG | 0.570605 | 8.1:-39945541 | MS.gene009155:CDS |
| TCTTGTTCGTGAGATCGCTC+AGG | 0.579084 | 8.1:-39945413 | MS.gene009155:CDS |
| TCACGAACAAGACGCTGGAA+AGG | 0.582899 | 8.1:+39945424 | None:intergenic |
| AACTCGCAACAAAAGCCGCT+CGG | 0.592966 | 8.1:-39945564 | MS.gene009155:CDS |
| CGATCTCACGAACAAGACGC+TGG | 0.602860 | 8.1:+39945419 | None:intergenic |
| TGTGTCGGCGCTGCAAGAAG+CGG | 0.603200 | 8.1:-39945356 | MS.gene009155:CDS |
| GTCGGCGCTGCAAGAAGCGG+CGG | 0.613578 | 8.1:-39945353 | MS.gene009155:CDS |
| ACCGCACGAAAATCCACCGG+CGG | 0.621620 | 8.1:-39945604 | MS.gene009155:CDS |
| AAGATCAAGCACGCTCGCCA+CGG | 0.626947 | 8.1:+39945230 | None:intergenic |
| CCACCGGCGGCAAAGCACCA+AGG | 0.632902 | 8.1:-39945591 | MS.gene009155:CDS |
| GCGGATCTCACGGAGTGCGA+CGG | 0.634415 | 8.1:+39945486 | None:intergenic |
| GTTCCTGGCCTGAAACGGTG+TGG | 0.654320 | 8.1:+39945508 | None:intergenic |
| CAGCTTGCTAGGCGTATCCG+TGG | 0.666195 | 8.1:-39945247 | MS.gene009155:CDS |
| CGCCGGAGCGGATTTCCGAG+CGG | 0.670325 | 8.1:+39945549 | None:intergenic |
| GATCGCTCAGGATTTCAAGA+CGG | 0.679659 | 8.1:-39945401 | MS.gene009155:CDS |
| CAAACCGCACGAAAATCCAC+CGG | 0.685560 | 8.1:-39945607 | MS.gene009155:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTATCTTGTTGGATTGTTTG+AGG | - | chr8.1:39945530-39945549 | MS.gene009155:CDS | 30.0% | |
| CTAGCAAGCTGAATATCCTT+AGG | + | chr8.1:39945600-39945619 | None:intergenic | 40.0% | |
| ! | CTAAGGATATTCAGCTTGCT+AGG | - | chr8.1:39945598-39945617 | MS.gene009155:CDS | 40.0% |
| AAGCTTCCGGATGAGTAGTT+CGG | + | chr8.1:39945412-39945431 | None:intergenic | 45.0% | |
| GATCGCTCAGGATTTCAAGA+CGG | - | chr8.1:39945455-39945474 | MS.gene009155:CDS | 45.0% | |
| TTTGTTGCGAGTTGCTTCCT+TGG | + | chr8.1:39945285-39945304 | None:intergenic | 45.0% | |
| ! | GCGTGTTACTATCATGCCTA+AGG | - | chr8.1:39945581-39945600 | MS.gene009155:CDS | 45.0% |
| ! | TTTTCAGAGCTCTGCTGTGT+CGG | - | chr8.1:39945485-39945504 | MS.gene009155:CDS | 45.0% |
| !! | ACACAGCAGAGCTCTGAAAA+CGG | + | chr8.1:39945485-39945504 | None:intergenic | 45.0% |
| AACTCGCAACAAAAGCCGCT+CGG | - | chr8.1:39945292-39945311 | MS.gene009155:CDS | 50.0% | |
| CAAACCGCACGAAAATCCAC+CGG | - | chr8.1:39945249-39945268 | MS.gene009155:CDS | 50.0% | |
| TCACGAACAAGACGCTGGAA+AGG | + | chr8.1:39945435-39945454 | None:intergenic | 50.0% | |
| TCTGGTACTTGCGGATCTCA+CGG | + | chr8.1:39945383-39945402 | None:intergenic | 50.0% | |
| TCTTGTTCGTGAGATCGCTC+AGG | - | chr8.1:39945443-39945462 | MS.gene009155:CDS | 50.0% | |
| TGAAGAAGCCACACCGTTTC+AGG | - | chr8.1:39945340-39945359 | MS.gene009155:CDS | 50.0% | |
| !! | AGAGCACCGAACTACTCATC+CGG | - | chr8.1:39945403-39945422 | MS.gene009155:CDS | 50.0% |
| !! | TGAGTAGTTCGGTGCTCTTC+TGG | + | chr8.1:39945401-39945420 | None:intergenic | 50.0% |
| CGATCTCACGAACAAGACGC+TGG | + | chr8.1:39945440-39945459 | None:intergenic | 55.0% | |
| CGGTGCTCTTCTGGTACTTG+CGG | + | chr8.1:39945392-39945411 | None:intergenic | 55.0% | |
| GACGCTGGAAAGGAAGCTTC+CGG | + | chr8.1:39945425-39945444 | None:intergenic | 55.0% | |
| AAGCCACACCGTTTCAGGCC+AGG | - | chr8.1:39945345-39945364 | MS.gene009155:CDS | 60.0% | |
| ACCGCACGAAAATCCACCGG+CGG | - | chr8.1:39945252-39945271 | MS.gene009155:CDS | 60.0% | |
| CGACGGTTCCTGGCCTGAAA+CGG | + | chr8.1:39945356-39945375 | None:intergenic | 60.0% | |
| GCGGCGGAGGCTTATCTTGT+TGG | - | chr8.1:39945519-39945538 | MS.gene009155:CDS | 60.0% | |
| GTGTGGCTTCTTCACTCCGC+CGG | + | chr8.1:39945334-39945353 | None:intergenic | 60.0% | |
| GTTCCTGGCCTGAAACGGTG+TGG | + | chr8.1:39945351-39945370 | None:intergenic | 60.0% | |
| ! | TGTGTCGGCGCTGCAAGAAG+CGG | - | chr8.1:39945500-39945519 | MS.gene009155:CDS | 60.0% |
| !! | CAGCTTGCTAGGCGTATCCG+TGG | - | chr8.1:39945609-39945628 | MS.gene009155:CDS | 60.0% |
| !! | CTTCCTTGGTGCTTTGCCGC+CGG | + | chr8.1:39945271-39945290 | None:intergenic | 60.0% |
| AGCCGCTCGGAAATCCGCTC+CGG | - | chr8.1:39945305-39945324 | MS.gene009155:CDS | 65.0% | |
| GCGGATCTCACGGAGTGCGA+CGG | + | chr8.1:39945373-39945392 | None:intergenic | 65.0% | |
| TCACGGAGTGCGACGGTTCC+TGG | + | chr8.1:39945366-39945385 | None:intergenic | 65.0% | |
| TTCTTCACTCCGCCGGTCGC+CGG | + | chr8.1:39945327-39945346 | None:intergenic | 65.0% | |
| !! | GCCGCCGGTGGATTTTCGTG+CGG | + | chr8.1:39945256-39945275 | None:intergenic | 65.0% |
| AAATCCGCTCCGGCGACCGG+CGG | - | chr8.1:39945315-39945334 | MS.gene009155:CDS | 70.0% | |
| CGGAAATCCGCTCCGGCGAC+CGG | - | chr8.1:39945312-39945331 | MS.gene009155:CDS | 70.0% | |
| GTCGGCGCTGCAAGAAGCGG+CGG | - | chr8.1:39945503-39945522 | MS.gene009155:CDS | 70.0% | |
| ! | CCACCGGCGGCAAAGCACCA+AGG | - | chr8.1:39945265-39945284 | MS.gene009155:CDS | 70.0% |
| ! | CGCCGGAGCGGATTTCCGAG+CGG | + | chr8.1:39945310-39945329 | None:intergenic | 70.0% |
| !! | CCTTGGTGCTTTGCCGCCGG+TGG | + | chr8.1:39945268-39945287 | None:intergenic | 70.0% |
| GGCGCTGCAAGAAGCGGCGG+AGG | - | chr8.1:39945506-39945525 | MS.gene009155:CDS | 75.0% | |
| ! | CACTCCGCCGGTCGCCGGAG+CGG | + | chr8.1:39945322-39945341 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 39945234 | 39945644 | 39945234 | ID=MS.gene009155 |
| chr8.1 | mRNA | 39945234 | 39945644 | 39945234 | ID=MS.gene009155.t1;Parent=MS.gene009155 |
| chr8.1 | exon | 39945234 | 39945644 | 39945234 | ID=MS.gene009155.t1.exon1;Parent=MS.gene009155.t1 |
| chr8.1 | CDS | 39945234 | 39945644 | 39945234 | ID=cds.MS.gene009155.t1;Parent=MS.gene009155.t1 |
| Gene Sequence |
| Protein sequence |