AlfalfaGEDB | MS.gene26084

M. sativa cultivar XinJiangDaYe / MS.gene26084

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26084.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26084.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26084.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene28438	MS.gene26084	PPI
MS.gene027907	MS.gene26084	PPI
MS.gene26084	MS.gene027909	PPI
MS.gene067742	MS.gene26084	PPI
MS.gene78322	MS.gene26084	PPI
MS.gene034255	MS.gene26084	PPI
MS.gene011711	MS.gene26084	PPI
MS.gene87898	MS.gene26084	PPI
MS.gene58116	MS.gene26084	PPI
MS.gene64998	MS.gene26084	PPI
MS.gene61192	MS.gene26084	PPI
MS.gene26084	MS.gene030664	PPI
MS.gene28432	MS.gene26084	PPI
MS.gene29445	MS.gene26084	PPI
MS.gene88233	MS.gene26084	PPI
MS.gene55626	MS.gene26084	PPI
MS.gene89203	MS.gene26084	PPI
MS.gene056335	MS.gene26084	PPI
MS.gene068179	MS.gene26084	PPI
MS.gene82109	MS.gene26084	PPI
MS.gene063067	MS.gene26084	PPI
MS.gene26084	MS.gene47284	PPI
MS.gene06236	MS.gene26084	PPI
MS.gene95244	MS.gene26084	PPI
MS.gene26084	MS.gene61193	PPI
MS.gene41679	MS.gene26084	PPI
MS.gene66752	MS.gene26084	PPI
MS.gene58114	MS.gene26084	PPI
MS.gene94307	MS.gene26084	PPI
MS.gene024064	MS.gene26084	PPI
MS.gene26084	MS.gene069975	PPI
MS.gene26084	MS.gene78324	PPI
MS.gene009155	MS.gene26084	PPI
MS.gene78924	MS.gene26084	PPI
MS.gene25357	MS.gene26084	PPI
MS.gene07616	MS.gene26084	PPI
MS.gene070538	MS.gene26084	PPI
MS.gene98552	MS.gene26084	PPI
MS.gene26084	MS.gene017431	PPI
MS.gene26084	MS.gene70258	PPI
MS.gene066535	MS.gene26084	PPI
MS.gene038612	MS.gene26084	PPI
MS.gene26084	MS.gene58112	PPI
MS.gene26084	MS.gene011710	PPI
MS.gene26084	MS.gene29300	PPI
MS.gene26084	MS.gene072432	PPI
MS.gene64164	MS.gene26084	PPI
MS.gene28434	MS.gene26084	PPI
MS.gene014301	MS.gene26084	PPI
MS.gene000604	MS.gene26084	PPI
MS.gene24196	MS.gene26084	PPI
MS.gene26084	MS.gene63118	PPI
MS.gene030661	MS.gene26084	PPI
MS.gene017431	MS.gene26084	PPI
MS.gene79799	MS.gene26084	PPI
MS.gene97703	MS.gene26084	PPI
MS.gene059054	MS.gene26084	PPI
MS.gene70990	MS.gene26084	PPI
MS.gene26084	MS.gene60196	PPI
MS.gene26084	MS.gene29298	PPI
MS.gene91631	MS.gene26084	PPI
MS.gene26084	MS.gene34797	PPI
MS.gene60199	MS.gene26084	PPI
MS.gene021887	MS.gene26084	PPI
MS.gene26084	MS.gene46303	PPI
MS.gene52605	MS.gene26084	PPI
MS.gene013163	MS.gene26084	PPI
MS.gene020313	MS.gene26084	PPI
MS.gene26084	MS.gene64996	PPI
MS.gene49838	MS.gene26084	PPI
MS.gene32514	MS.gene26084	PPI
MS.gene78323	MS.gene26084	PPI
MS.gene44458	MS.gene26084	PPI
MS.gene26084	MS.gene78227	PPI
MS.gene028296	MS.gene26084	PPI
MS.gene39538	MS.gene26084	PPI
MS.gene29299	MS.gene26084	PPI
MS.gene26084	MS.gene066536	PPI
MS.gene042871	MS.gene26084	PPI
MS.gene78922	MS.gene26084	PPI
MS.gene66836	MS.gene26084	PPI
MS.gene033505	MS.gene26084	PPI
MS.gene071851	MS.gene26084	PPI
MS.gene47573	MS.gene26084	PPI
MS.gene50129	MS.gene26084	PPI
MS.gene32513	MS.gene26084	PPI
MS.gene016712	MS.gene26084	PPI
MS.gene68716	MS.gene26084	PPI
MS.gene030666	MS.gene26084	PPI
MS.gene26084	MS.gene35163	PPI
MS.gene066537	MS.gene26084	PPI
MS.gene97701	MS.gene26084	PPI
MS.gene057458	MS.gene26084	PPI
MS.gene94315	MS.gene26084	PPI
MS.gene26084	MS.gene78224	PPI
MS.gene36496	MS.gene26084	PPI
MS.gene027904	MS.gene26084	PPI
MS.gene00233	MS.gene26084	PPI
MS.gene29521	MS.gene26084	PPI
MS.gene014181	MS.gene26084	PPI
MS.gene26084	MS.gene81646	PPI
MS.gene018762	MS.gene26084	PPI
MS.gene05905	MS.gene26084	PPI
MS.gene068178	MS.gene26084	PPI
MS.gene44457	MS.gene26084	PPI
MS.gene26084	MS.gene70990	PPI
MS.gene26084	MS.gene28438	PPI
MS.gene68113	MS.gene26084	PPI
MS.gene26084	MS.gene70261	PPI
MS.gene053230	MS.gene26084	PPI
MS.gene064876	MS.gene26084	PPI
MS.gene26084	MS.gene066960	PPI
MS.gene66051	MS.gene26084	PPI
MS.gene04820	MS.gene26084	PPI
MS.gene26084	MS.gene072431	PPI
MS.gene072433	MS.gene26084	PPI
MS.gene65125	MS.gene26084	PPI
MS.gene26084	MS.gene03849	PPI
MS.gene26084	MS.gene04748	PPI
MS.gene23886	MS.gene26084	PPI
MS.gene046235	MS.gene26084	PPI
MS.gene26406	MS.gene26084	PPI
MS.gene60200	MS.gene26084	PPI
MS.gene015760	MS.gene26084	PPI
MS.gene055512	MS.gene26084	PPI
MS.gene26084	MS.gene05905	PPI
MS.gene049111	MS.gene26084	PPI
MS.gene70263	MS.gene26084	PPI
MS.gene34797	MS.gene26084	PPI
MS.gene58285	MS.gene26084	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26084.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene26084.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene26084.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene26084.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene26084.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene26084.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene26084.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene26084.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene26084.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene26084.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene26084.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene26084.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene26084.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene26084.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene26084.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene26084.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene26084.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene26084.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene26084.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene26084.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene26084.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene26084.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene26084.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene26084.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26084.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene26084.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene26084.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene26084.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene26084.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene26084.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene26084.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene26084.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene26084.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene26084.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene26084.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene26084.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene26084.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene26084.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene26084.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene26084.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACGAAGAGCGACTGTTCC+TGG	0.301860	5.4:-56272117	None:intergenic
CGATCTCTCTAACCAATCTT+TGG	0.311682	5.4:-56272191	None:intergenic
GCAGCTGAAGCTTATCTTGT+TGG	0.346066	5.4:+56272273	MS.gene26084:CDS
AGCTGATTTCCGAGCGGCTT+TGG	0.356003	5.4:-56272055	None:intergenic
GAAGCTTCCTTTCCAAAGAT+TGG	0.376805	5.4:+56272179	MS.gene26084:CDS
CGGAAATCAGCTCCGGCGAC+TGG	0.383526	5.4:+56272066	MS.gene26084:CDS
GAGCAGAAACAGCGCTACTC+TGG	0.433957	5.4:-56272242	None:intergenic
TTGGTGGCGAGCTGTTTCCT+TGG	0.436325	5.4:-56272036	None:intergenic
TTCTTCACTCCTCCAGTCGC+CGG	0.441465	5.4:-56272078	None:intergenic
AAGCCCTCTCGCCTCTGATT+CGG	0.461144	5.4:-56272374	None:intergenic
AAACAGCGCTACTCTGGAAT+CGG	0.489842	5.4:-56272236	None:intergenic
AGCATGAATAGCACAAAGAT+TGG	0.492949	5.4:-56272310	None:intergenic
TGATTTCCGAGCGGCTTTGG+TGG	0.495094	5.4:-56272052	None:intergenic
CGAGCAAGTTGAATATCCTT+AGG	0.498424	5.4:-56272351	None:intergenic
AGCCGCTCGGAAATCAGCTC+CGG	0.498921	5.4:+56272059	MS.gene26084:CDS
GAGAGTTACTATTATGCCTA+AGG	0.517685	5.4:+56272335	MS.gene26084:CDS
AGAGCACAGAGCTTCTCATA+AGG	0.533669	5.4:+56272157	MS.gene26084:CDS
CAACTTGCTCGCCGAATCAG+AGG	0.534605	5.4:+56272363	MS.gene26084:CDS
AGCTCGCCACCAAAGCCGCT+CGG	0.547091	5.4:+56272046	MS.gene26084:CDS
TCTCTAACCAATCTTTGGAA+AGG	0.575485	5.4:-56272186	None:intergenic
GATCGCTCAGGATTTCAAAA+CGG	0.577579	5.4:+56272209	MS.gene26084:CDS
TCGCCGAATCAGAGGCGAGA+GGG	0.598725	5.4:+56272371	MS.gene26084:CDS
CAAACAGCAAGAAAATCCAC+CGG	0.620184	5.4:+56272003	MS.gene26084:CDS
ATTGGTTAGAGAGATCGCTC+AGG	0.627716	5.4:+56272197	MS.gene26084:CDS
AAATCAGCTCCGGCGACTGG+AGG	0.630058	5.4:+56272069	MS.gene26084:CDS
AAGCCACACAGATTCCGTCC+AGG	0.632383	5.4:+56272099	MS.gene26084:CDS
CTCGCCGAATCAGAGGCGAG+AGG	0.633846	5.4:+56272370	MS.gene26084:CDS
GAAGAGCGACTGTTCCTGGA+CGG	0.668213	5.4:-56272113	None:intergenic
GTTCCTGGACGGAATCTGTG+TGG	0.677012	5.4:-56272102	None:intergenic
CCACCGGAGGAAAAGCACCA+AGG	0.683879	5.4:+56272019	MS.gene26084:CDS
ACAGCAAGAAAATCCACCGG+AGG	0.684797	5.4:+56272006	MS.gene26084:CDS
CGCCGGAGCTGATTTCCGAG+CGG	0.685230	5.4:-56272061	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGCATGAATAGCACAAAGAT+TGG	-	chr5.4:56272313-56272332	None:intergenic	35.0%
	GAGAGTTACTATTATGCCTA+AGG	+	chr5.4:56272335-56272354	MS.gene26084:CDS	35.0%
!	TCTCTAACCAATCTTTGGAA+AGG	-	chr5.4:56272189-56272208	None:intergenic	35.0%
!!!	GGATTTTCTTGCTGTTTGTT+TGG	-	chr5.4:56272001-56272020	None:intergenic	35.0%
	CAAACAGCAAGAAAATCCAC+CGG	+	chr5.4:56272003-56272022	MS.gene26084:CDS	40.0%
	CGATCTCTCTAACCAATCTT+TGG	-	chr5.4:56272194-56272213	None:intergenic	40.0%
	GAAGCTTCCTTTCCAAAGAT+TGG	+	chr5.4:56272179-56272198	MS.gene26084:CDS	40.0%
	GATCGCTCAGGATTTCAAAA+CGG	+	chr5.4:56272209-56272228	MS.gene26084:CDS	40.0%
!	CGAGCAAGTTGAATATCCTT+AGG	-	chr5.4:56272354-56272373	None:intergenic	40.0%
	AGAGCACAGAGCTTCTCATA+AGG	+	chr5.4:56272157-56272176	MS.gene26084:CDS	45.0%
	ATTGGTTAGAGAGATCGCTC+AGG	+	chr5.4:56272197-56272216	MS.gene26084:CDS	45.0%
	GCAGCTGAAGCTTATCTTGT+TGG	+	chr5.4:56272273-56272292	MS.gene26084:CDS	45.0%
!!	AAACAGCGCTACTCTGGAAT+CGG	-	chr5.4:56272239-56272258	None:intergenic	45.0%
!!!	TTTCCTTGGTGCTTTTCCTC+CGG	-	chr5.4:56272025-56272044	None:intergenic	45.0%
	ACAGCAAGAAAATCCACCGG+AGG	+	chr5.4:56272006-56272025	MS.gene26084:CDS	50.0%
	AAGCCACACAGATTCCGTCC+AGG	+	chr5.4:56272099-56272118	MS.gene26084:CDS	55.0%
	AAGCCCTCTCGCCTCTGATT+CGG	-	chr5.4:56272377-56272396	None:intergenic	55.0%
	GAAGAGCGACTGTTCCTGGA+CGG	-	chr5.4:56272116-56272135	None:intergenic	55.0%
	GAGCAGAAACAGCGCTACTC+TGG	-	chr5.4:56272245-56272264	None:intergenic	55.0%
	GTTCCTGGACGGAATCTGTG+TGG	-	chr5.4:56272105-56272124	None:intergenic	55.0%
	TCACGAAGAGCGACTGTTCC+TGG	-	chr5.4:56272120-56272139	None:intergenic	55.0%
	TTCTTCACTCCTCCAGTCGC+CGG	-	chr5.4:56272081-56272100	None:intergenic	55.0%
!	AGCTGATTTCCGAGCGGCTT+TGG	-	chr5.4:56272058-56272077	None:intergenic	55.0%
!	CAACTTGCTCGCCGAATCAG+AGG	+	chr5.4:56272363-56272382	MS.gene26084:CDS	55.0%
!	TGATTTCCGAGCGGCTTTGG+TGG	-	chr5.4:56272055-56272074	None:intergenic	55.0%
!!	TTGGTGGCGAGCTGTTTCCT+TGG	-	chr5.4:56272039-56272058	None:intergenic	55.0%
	AAATCAGCTCCGGCGACTGG+AGG	+	chr5.4:56272069-56272088	MS.gene26084:CDS	60.0%
	AGCCGCTCGGAAATCAGCTC+CGG	+	chr5.4:56272059-56272078	MS.gene26084:CDS	60.0%
	TCGCCGAATCAGAGGCGAGA+GGG	+	chr5.4:56272371-56272390	MS.gene26084:CDS	60.0%
!!	CCACCGGAGGAAAAGCACCA+AGG	+	chr5.4:56272019-56272038	MS.gene26084:CDS	60.0%
!!!	CCTTGGTGCTTTTCCTCCGG+TGG	-	chr5.4:56272022-56272041	None:intergenic	60.0%
	AGCTCGCCACCAAAGCCGCT+CGG	+	chr5.4:56272046-56272065	MS.gene26084:CDS	65.0%
	CGGAAATCAGCTCCGGCGAC+TGG	+	chr5.4:56272066-56272085	MS.gene26084:CDS	65.0%
	CTCGCCGAATCAGAGGCGAG+AGG	+	chr5.4:56272370-56272389	MS.gene26084:CDS	65.0%
!	CGCCGGAGCTGATTTCCGAG+CGG	-	chr5.4:56272064-56272083	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	56271988	56272398	56271988	ID=MS.gene26084
chr5.4	mRNA	56271988	56272398	56271988	ID=MS.gene26084.t1;Parent=MS.gene26084
chr5.4	exon	56271988	56272398	56271988	ID=MS.gene26084.t1.exon1;Parent=MS.gene26084.t1
chr5.4	CDS	56271988	56272398	56271988	ID=cds.MS.gene26084.t1;Parent=MS.gene26084.t1

Gene Sequence

>MS.gene26084

ATGGCACGTACCAAACAAACAGCAAGAAAATCCACCGGAGGAAAAGCACCAAGGAAACAGCTCGCCACCAAAGCCGCTCGGAAATCAGCTCCGGCGACTGGAGGAGTGAAGAAGCCACACAGATTCCGTCCAGGAACAGTCGCTCTTCGTGAAATCAGAAAGTATCAGAAGAGCACAGAGCTTCTCATAAGGAAGCTTCCTTTCCAAAGATTGGTTAGAGAGATCGCTCAGGATTTCAAAACGGATCTCCGATTCCAGAGTAGCGCTGTTTCTGCTCTTCAAGAAGCAGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACCAATCTTTGTGCTATTCATGCTAAGAGAGTTACTATTATGCCTAAGGATATTCAACTTGCTCGCCGAATCAGAGGCGAGAGGGCTTAG

Protein sequence

>MS.gene26084.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA