AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071851


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071851.t1 MTR_7g059070 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_5g029820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_5g029770 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_4g065990 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_4g088150 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_2g035230 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_8g092720 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_8g063500 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_8g103245 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_8g092820 100.000 136 0 0 1 136 1 136 4.79e-96 272
MS.gene071851.t1 MTR_7g013610 100.000 136 0 0 1 136 100 235 1.10e-95 275
MS.gene071851.t1 MTR_4g097175 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene071851.t1 MTR_1g023630 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene071851.t1 MTR_8g061940 97.059 136 4 0 1 136 1 136 3.29e-93 265
MS.gene071851.t1 MTR_8g099430 96.324 136 5 0 1 136 1 136 1.38e-92 264
MS.gene071851.t1 MTR_4g097170 96.324 136 5 0 1 136 42 177 2.29e-91 262
MS.gene071851.t1 MTR_2g082310 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene071851.t1 MTR_2g082340 89.706 136 14 0 1 136 1 136 1.38e-86 248
MS.gene071851.t1 MTR_2g082370 85.616 146 11 1 1 136 1 146 1.74e-82 238
MS.gene071851.t1 MTR_2g082400 84.559 136 19 2 1 135 1 135 6.38e-75 219
MS.gene071851.t1 MTR_7g013600 86.607 112 15 0 25 136 2 113 1.01e-65 195
MS.gene071851.t1 MTR_7g023830 67.164 134 43 1 1 134 1 133 6.48e-56 171
MS.gene071851.t1 MTR_1g023630 73.504 117 15 3 1 111 1 107 3.81e-52 160
MS.gene071851.t1 MTR_5g024630 67.241 116 35 2 1 116 1 113 1.63e-45 144
MS.gene071851.t1 MTR_7g023880 58.647 133 41 3 3 134 18 137 5.46e-43 139
MS.gene071851.t1 MTR_5g024530 68.182 110 12 2 19 128 68 154 4.56e-42 137
MS.gene071851.t1 MTR_0197s0100 100.000 66 0 0 1 66 1 66 1.45e-40 132
MS.gene071851.t1 MTR_8g027840 55.102 98 43 1 37 133 28 125 4.83e-30 105
MS.gene071851.t1 MTR_8g105150 53.846 91 10 1 37 127 11 69 1.43e-25 92.4
MS.gene071851.t1 MTR_8g092740 97.368 38 1 0 99 136 118 155 4.60e-20 80.9
MS.gene071851.t1 MTR_5g089120 76.190 42 10 0 82 123 6 47 6.07e-18 73.6
MS.gene071851.t1 MTR_5g024600 62.963 54 20 0 1 54 1 54 1.76e-15 66.2
MS.gene071851.t1 MTR_4g128040 75.676 37 9 0 79 115 20 56 3.38e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071851.t1 AT5G65360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene071851.t1 AT5G10390 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene071851.t1 AT5G10400 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene071851.t1 AT3G27360 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene071851.t1 AT1G09200 99.265 136 1 0 1 136 1 136 1.55e-95 271
MS.gene071851.t1 AT5G10980 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40040 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40030 97.059 136 4 0 1 136 1 136 3.44e-93 265
MS.gene071851.t1 AT4G40030 97.059 136 4 0 1 136 29 164 6.76e-93 266
MS.gene071851.t1 AT5G65350 94.853 136 7 0 1 136 1 136 5.09e-91 260
MS.gene071851.t1 AT1G75600 93.382 136 9 0 1 136 1 136 1.20e-89 256
MS.gene071851.t1 AT1G13370 93.382 136 9 0 1 136 1 136 5.32e-89 254
MS.gene071851.t1 AT1G19890 89.781 137 13 1 1 136 1 137 1.03e-83 241
MS.gene071851.t1 AT5G12910 71.111 135 34 2 1 135 1 130 9.51e-67 198
MS.gene071851.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene071851.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene071851.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110
MS.gene071851.t1 AT1G01370 53.982 113 49 2 24 134 63 174 2.86e-31 110

Find 35 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAGCTCAGTGCTCTTC+TGG 0.304985 2.2:+53585440 None:intergenic
TCACGAAGTGCGACGGTTCC+TGG 0.318785 2.2:+53585475 None:intergenic
GCTGCTGAAGCTTATCTTGT+TGG 0.357332 2.2:-53585319 MS.gene071851:CDS
GAAGCTTCCGTTTCAAAGAT+TGG 0.362056 2.2:-53585413 MS.gene071851:CDS
GGATTTGCGGGCTGTTTGTT+TGG 0.425629 2.2:+53585594 None:intergenic
CGGAAATCCGCTCCGGCGAC+CGG 0.429737 2.2:-53585526 MS.gene071851:CDS
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 2.2:+53585514 None:intergenic
GAGCAGAAACAGCACTACTC+TGG 0.498444 2.2:+53585350 None:intergenic
TCTCGAACCAATCTTTGAAA+CGG 0.505611 2.2:+53585406 None:intergenic
CGACGGTTCCTGGACGGAAA+CGG 0.511490 2.2:+53585485 None:intergenic
TTGTGCTATTCATGCTAAGA+GGG 0.513784 2.2:-53585275 MS.gene071851:CDS
CACTCCTCCGGTCGCCGGAG+CGG 0.515017 2.2:+53585519 None:intergenic
AGCCGCTCGGAAATCCGCTC+CGG 0.515753 2.2:-53585533 MS.gene071851:CDS
CAAACAGCCCGCAAATCCAC+CGG 0.524480 2.2:-53585589 MS.gene071851:CDS
AAGCCACACCGTTTCCGTCC+AGG 0.524906 2.2:-53585493 MS.gene071851:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.526132 2.2:+53585507 None:intergenic
AATTGCTCAGGATTTCAAAA+CGG 0.529430 2.2:-53585383 MS.gene071851:CDS
ATTGGTTCGAGAAATTGCTC+AGG 0.529480 2.2:-53585395 MS.gene071851:CDS
AGAGCACTGAGCTTCTCATA+AGG 0.531497 2.2:-53585435 MS.gene071851:CDS
AAACAGCACTACTCTGGAAA+CGG 0.532210 2.2:+53585356 None:intergenic
GAGGGTTACTATTATGCCTA+AGG 0.533362 2.2:-53585257 MS.gene071851:CDS
CTCGCAAGCTGAATATCCTT+AGG 0.533935 2.2:+53585241 None:intergenic
TTTGTGCTATTCATGCTAAG+AGG 0.537531 2.2:-53585276 MS.gene071851:CDS
CAGTGCTCTTCTGGTACTTG+CGG 0.559165 2.2:+53585449 None:intergenic
GAGAAGAATCAGAGGCGAAA+GGG 0.568256 2.2:-53585221 MS.gene071851:CDS
CGAGAAGAATCAGAGGCGAA+AGG 0.575054 2.2:-53585222 MS.gene071851:CDS
GCGGATTTCACGAAGTGCGA+CGG 0.598942 2.2:+53585468 None:intergenic
AACTCGCCACAAAAGCCGCT+CGG 0.605384 2.2:-53585546 MS.gene071851:CDS
AAATCCGCTCCGGCGACCGG+AGG 0.609902 2.2:-53585523 MS.gene071851:CDS
GTTCCTGGACGGAAACGGTG+TGG 0.651488 2.2:+53585490 None:intergenic
GAAGTGCGACGGTTCCTGGA+CGG 0.655834 2.2:+53585479 None:intergenic
ACAGCCCGCAAATCCACCGG+CGG 0.662314 2.2:-53585586 MS.gene071851:CDS
CAGCTTGCGAGAAGAATCAG+AGG 0.668283 2.2:-53585229 MS.gene071851:CDS
CGCCGGAGCGGATTTCCGAG+CGG 0.670325 2.2:+53585531 None:intergenic
TTTGTGGCGAGTTGTTTGCG+TGG 0.711662 2.2:+53585556 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATTGCTCAGGATTTCAAAA+CGG - chr2.2:53585437-53585456 MS.gene071851:CDS 30.0%
! TCTCGAACCAATCTTTGAAA+CGG + chr2.2:53585417-53585436 None:intergenic 35.0%
! TTGTGCTATTCATGCTAAGA+GGG - chr2.2:53585545-53585564 MS.gene071851:CDS 35.0%
! TTTGTGCTATTCATGCTAAG+AGG - chr2.2:53585544-53585563 MS.gene071851:CDS 35.0%
GAAGCTTCCGTTTCAAAGAT+TGG - chr2.2:53585407-53585426 MS.gene071851:CDS 40.0%
GAGGGTTACTATTATGCCTA+AGG - chr2.2:53585563-53585582 MS.gene071851:CDS 40.0%
! AAACAGCACTACTCTGGAAA+CGG + chr2.2:53585467-53585486 None:intergenic 40.0%
!! ATTGGTTCGAGAAATTGCTC+AGG - chr2.2:53585425-53585444 MS.gene071851:CDS 40.0%
CTCGCAAGCTGAATATCCTT+AGG + chr2.2:53585582-53585601 None:intergenic 45.0%
GAGAAGAATCAGAGGCGAAA+GGG - chr2.2:53585599-53585618 MS.gene071851:CDS 45.0%
GCTGCTGAAGCTTATCTTGT+TGG - chr2.2:53585501-53585520 MS.gene071851:CDS 45.0%
!! AGAGCACTGAGCTTCTCATA+AGG - chr2.2:53585385-53585404 MS.gene071851:CDS 45.0%
CAGCTTGCGAGAAGAATCAG+AGG - chr2.2:53585591-53585610 MS.gene071851:CDS 50.0%
CAGTGCTCTTCTGGTACTTG+CGG + chr2.2:53585374-53585393 None:intergenic 50.0%
CGAGAAGAATCAGAGGCGAA+AGG - chr2.2:53585598-53585617 MS.gene071851:CDS 50.0%
GAGCAGAAACAGCACTACTC+TGG + chr2.2:53585473-53585492 None:intergenic 50.0%
TGAGAAGCTCAGTGCTCTTC+TGG + chr2.2:53585383-53585402 None:intergenic 50.0%
TTTGTGGCGAGTTGTTTGCG+TGG + chr2.2:53585267-53585286 None:intergenic 50.0%
! GGATTTGCGGGCTGTTTGTT+TGG + chr2.2:53585229-53585248 None:intergenic 50.0%
AACTCGCCACAAAAGCCGCT+CGG - chr2.2:53585274-53585293 MS.gene071851:CDS 55.0%
CAAACAGCCCGCAAATCCAC+CGG - chr2.2:53585231-53585250 MS.gene071851:CDS 55.0%
GCGGATTTCACGAAGTGCGA+CGG + chr2.2:53585355-53585374 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG + chr2.2:53585316-53585335 None:intergenic 55.0%
! GGATTTCCGAGCGGCTTTTG+TGG + chr2.2:53585283-53585302 None:intergenic 55.0%
! TTTGCGTGGTGCTTTTCCGC+CGG + chr2.2:53585253-53585272 None:intergenic 55.0%
!! CTTTTCCGCCGGTGGATTTG+CGG + chr2.2:53585242-53585261 None:intergenic 55.0%
!! TTTTCCGCCGGTGGATTTGC+GGG + chr2.2:53585241-53585260 None:intergenic 55.0%
AAGCCACACCGTTTCCGTCC+AGG - chr2.2:53585327-53585346 MS.gene071851:CDS 60.0%
CGACGGTTCCTGGACGGAAA+CGG + chr2.2:53585338-53585357 None:intergenic 60.0%
GAAGTGCGACGGTTCCTGGA+CGG + chr2.2:53585344-53585363 None:intergenic 60.0%
GTTCCTGGACGGAAACGGTG+TGG + chr2.2:53585333-53585352 None:intergenic 60.0%
TCACGAAGTGCGACGGTTCC+TGG + chr2.2:53585348-53585367 None:intergenic 60.0%
TTCTTCACTCCTCCGGTCGC+CGG + chr2.2:53585309-53585328 None:intergenic 60.0%
ACAGCCCGCAAATCCACCGG+CGG - chr2.2:53585234-53585253 MS.gene071851:CDS 65.0%
AGCCGCTCGGAAATCCGCTC+CGG - chr2.2:53585287-53585306 MS.gene071851:CDS 65.0%
AAATCCGCTCCGGCGACCGG+AGG - chr2.2:53585297-53585316 MS.gene071851:CDS 70.0%
CGGAAATCCGCTCCGGCGAC+CGG - chr2.2:53585294-53585313 MS.gene071851:CDS 70.0%
! CGCCGGAGCGGATTTCCGAG+CGG + chr2.2:53585292-53585311 None:intergenic 70.0%
! GCGTGGTGCTTTTCCGCCGG+TGG + chr2.2:53585250-53585269 None:intergenic 70.0%
CACTCCTCCGGTCGCCGGAG+CGG + chr2.2:53585304-53585323 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 53585216 53585626 53585216 ID=MS.gene071851
chr2.2 mRNA 53585216 53585626 53585216 ID=MS.gene071851.t1;Parent=MS.gene071851
chr2.2 exon 53585216 53585626 53585216 ID=MS.gene071851.t1.exon1;Parent=MS.gene071851.t1
chr2.2 CDS 53585216 53585626 53585216 ID=cds.MS.gene071851.t1;Parent=MS.gene071851.t1
Gene Sequence

>MS.gene071851

ATGGCACGTACCAAACAAACAGCCCGCAAATCCACCGGCGGAAAAGCACCACGCAAACAACTCGCCACAAAAGCCGCTCGGAAATCCGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACCGTTTCCGTCCAGGAACCGTCGCACTTCGTGAAATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCGTTTCAAAGATTGGTTCGAGAAATTGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAGCTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>MS.gene071851.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA