AlfalfaGEDB | MS.gene068179

M. sativa cultivar XinJiangDaYe / MS.gene068179

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068179.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068179.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068179.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene60199	MS.gene068179	PPI
MS.gene068179	MS.gene89203	PPI
MS.gene021887	MS.gene068179	PPI
MS.gene013163	MS.gene068179	PPI
MS.gene020313	MS.gene068179	PPI
MS.gene068179	MS.gene00233	PPI
MS.gene068179	MS.gene63118	PPI
MS.gene32514	MS.gene068179	PPI
MS.gene053230	MS.gene068179	PPI
MS.gene064876	MS.gene068179	PPI
MS.gene44458	MS.gene068179	PPI
MS.gene028296	MS.gene068179	PPI
MS.gene068179	MS.gene47573	PPI
MS.gene042871	MS.gene068179	PPI
MS.gene04820	MS.gene068179	PPI
MS.gene66836	MS.gene068179	PPI
MS.gene072433	MS.gene068179	PPI
MS.gene068179	MS.gene47284	PPI
MS.gene068179	MS.gene60196	PPI
MS.gene68716	MS.gene068179	PPI
MS.gene068179	MS.gene61193	PPI
MS.gene030666	MS.gene068179	PPI
MS.gene068179	MS.gene34797	PPI
MS.gene066537	MS.gene068179	PPI
MS.gene97701	MS.gene068179	PPI
MS.gene057458	MS.gene068179	PPI
MS.gene068179	MS.gene26084	PPI
MS.gene068179	MS.gene78227	PPI
MS.gene068179	MS.gene063067	PPI
MS.gene36496	MS.gene068179	PPI
MS.gene068179	MS.gene65125	PPI
MS.gene049111	MS.gene068179	PPI
MS.gene027904	MS.gene068179	PPI
MS.gene00233	MS.gene068179	PPI
MS.gene29521	MS.gene068179	PPI
MS.gene014181	MS.gene068179	PPI
MS.gene068179	MS.gene23886	PPI
MS.gene068179	MS.gene29299	PPI
MS.gene068179	MS.gene015760	PPI
MS.gene068178	MS.gene068179	PPI
MS.gene44457	MS.gene068179	PPI
MS.gene068179	MS.gene35163	PPI
MS.gene68113	MS.gene068179	PPI
MS.gene068179	MS.gene78224	PPI
MS.gene66051	MS.gene068179	PPI
MS.gene034255	MS.gene068179	PPI
MS.gene068179	MS.gene038612	PPI
MS.gene011711	MS.gene068179	PPI
MS.gene87898	MS.gene068179	PPI
MS.gene068179	MS.gene033505	PPI
MS.gene068179	MS.gene29298	PPI
MS.gene068179	MS.gene66752	PPI
MS.gene29445	MS.gene068179	PPI
MS.gene046235	MS.gene068179	PPI
MS.gene068179	MS.gene46303	PPI
MS.gene068179	MS.gene28438	PPI
MS.gene068179	MS.gene64996	PPI
MS.gene82109	MS.gene068179	PPI
MS.gene068179	MS.gene066960	PPI
MS.gene60200	MS.gene068179	PPI
MS.gene015760	MS.gene068179	PPI
MS.gene055512	MS.gene068179	PPI
MS.gene068179	MS.gene066536	PPI
MS.gene024064	MS.gene068179	PPI
MS.gene70263	MS.gene068179	PPI
MS.gene068179	MS.gene072431	PPI
MS.gene58285	MS.gene068179	PPI
MS.gene068179	MS.gene78323	PPI
MS.gene068179	MS.gene91631	PPI
MS.gene068179	MS.gene58114	PPI
MS.gene068179	MS.gene03849	PPI
MS.gene068179	MS.gene04748	PPI
MS.gene068179	MS.gene50129	PPI
MS.gene038612	MS.gene068179	PPI
MS.gene068179	MS.gene49838	PPI
MS.gene027907	MS.gene068179	PPI
MS.gene067742	MS.gene068179	PPI
MS.gene78322	MS.gene068179	PPI
MS.gene014301	MS.gene068179	PPI
MS.gene068179	MS.gene32513	PPI
MS.gene068179	MS.gene64164	PPI
MS.gene58116	MS.gene068179	PPI
MS.gene068179	MS.gene41679	PPI
MS.gene64998	MS.gene068179	PPI
MS.gene030661	MS.gene068179	PPI
MS.gene068179	MS.gene81646	PPI
MS.gene61192	MS.gene068179	PPI
MS.gene28432	MS.gene068179	PPI
MS.gene068179	MS.gene26406	PPI
MS.gene068179	MS.gene70990	PPI
MS.gene068179	MS.gene24196	PPI
MS.gene88233	MS.gene068179	PPI
MS.gene97703	MS.gene068179	PPI
MS.gene068179	MS.gene027909	PPI
MS.gene068179	MS.gene70261	PPI
MS.gene06236	MS.gene068179	PPI
MS.gene95244	MS.gene068179	PPI
MS.gene94307	MS.gene068179	PPI
MS.gene068179	MS.gene018762	PPI
MS.gene91631	MS.gene068179	PPI
MS.gene009155	MS.gene068179	PPI
MS.gene78924	MS.gene068179	PPI
MS.gene52605	MS.gene068179	PPI
MS.gene068179	MS.gene64998	PPI
MS.gene25357	MS.gene068179	PPI
MS.gene07616	MS.gene068179	PPI
MS.gene070538	MS.gene068179	PPI
MS.gene98552	MS.gene068179	PPI
MS.gene068179	MS.gene030664	PPI
MS.gene39538	MS.gene068179	PPI
MS.gene066535	MS.gene068179	PPI
MS.gene78922	MS.gene068179	PPI
MS.gene068179	MS.gene94315	PPI
MS.gene071851	MS.gene068179	PPI
MS.gene28434	MS.gene068179	PPI
MS.gene068179	MS.gene55626	PPI
MS.gene016712	MS.gene068179	PPI
MS.gene068179	MS.gene05905	PPI
MS.gene068179	MS.gene069975	PPI
MS.gene000604	MS.gene068179	PPI
MS.gene068179	MS.gene78324	PPI
MS.gene068179	MS.gene057458	PPI
MS.gene068179	MS.gene017431	PPI
MS.gene068179	MS.gene70258	PPI
MS.gene79799	MS.gene068179	PPI
MS.gene068179	MS.gene056335	PPI
MS.gene068179	MS.gene58112	PPI
MS.gene068179	MS.gene011710	PPI
MS.gene068179	MS.gene29300	PPI
MS.gene059054	MS.gene068179	PPI
MS.gene068179	MS.gene072432	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068179.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene068179.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene068179.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene068179.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene068179.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene068179.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene068179.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene068179.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene068179.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene068179.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene068179.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene068179.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene068179.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene068179.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene068179.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene068179.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene068179.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene068179.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene068179.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene068179.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene068179.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene068179.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene068179.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene068179.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068179.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene068179.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene068179.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene068179.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene068179.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene068179.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene068179.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene068179.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene068179.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene068179.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene068179.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene068179.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene068179.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene068179.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene068179.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene068179.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 0 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATCGCTCAGGATTTCAAAA+CGG	-	15173:673-692	MS.gene068179:CDS	35.0%
	GAAGCTTCCATTTCAAAGAT+TGG	-	15173:643-662	MS.gene068179:CDS	35.0%
!	TCTCGAACCAATCTTTGAAA+TGG	+	15173:653-672	MS.gene068179:intergenic	35.0%
!	TTGTGCTATTCATGCTAAGA+GGG	-	15173:781-800	MS.gene068179:CDS	35.0%
!	TTTGTGCTATTCATGCTAAG+AGG	-	15173:780-799	MS.gene068179:CDS	35.0%
	GAGGGTTACTATTATGCCTA+AGG	-	15173:799-818	MS.gene068179:CDS	40.0%
!	AAACAGCACTACTCTGGAAA+CGG	+	15173:703-722	MS.gene068179:intergenic	40.0%
!	CTCGCAAGTTGAATATCCTT+AGG	+	15173:818-837	MS.gene068179:intergenic	40.0%
	CAACTTGCGAGAAGAATCAG+AGG	-	15173:827-846	MS.gene068179:CDS	45.0%
	GAGAAGAATCAGAGGCGAAA+GGG	-	15173:835-854	MS.gene068179:CDS	45.0%
	GCTGCTGAAGCTTATCTTGT+TGG	-	15173:737-756	MS.gene068179:CDS	45.0%
!!	AGAGCACTGAGCTTCTCATA+AGG	-	15173:621-640	MS.gene068179:CDS	45.0%
!!	ATTGGTTCGAGAAATCGCTC+AGG	-	15173:661-680	MS.gene068179:CDS	45.0%
	CAGTGCTCTTCTGGTACTTG+CGG	+	15173:610-629	MS.gene068179:intergenic	50.0%
	CGAGAAGAATCAGAGGCGAA+AGG	-	15173:834-853	MS.gene068179:CDS	50.0%
	GAGCAGAAACAGCACTACTC+TGG	+	15173:709-728	MS.gene068179:intergenic	50.0%
	TGAGAAGCTCAGTGCTCTTC+TGG	+	15173:619-638	MS.gene068179:intergenic	50.0%
	TTTGTGGCGAGTTGTTTGCG+TGG	+	15173:503-522	MS.gene068179:intergenic	50.0%
!!	CTTTTCCGCCGGTGGATTTT+CGG	+	15173:478-497	MS.gene068179:intergenic	50.0%
!!	GGATTTTCGGGCGGTTTGTT+TGG	+	15173:465-484	MS.gene068179:intergenic	50.0%
!!	TTTTCCGCCGGTGGATTTTC+GGG	+	15173:477-496	MS.gene068179:intergenic	50.0%
	AACTCGCCACAAAAGCCGCT+CGG	-	15173:510-529	MS.gene068179:CDS	55.0%
	CAAACCGCCCGAAAATCCAC+CGG	-	15173:467-486	MS.gene068179:CDS	55.0%
	GCGGATTTCACGAAGTGCGA+CGG	+	15173:591-610	MS.gene068179:intergenic	55.0%
!	GGATTTCCGAGCGGCTTTTG+TGG	+	15173:519-538	MS.gene068179:intergenic	55.0%
!	TTTGCGTGGTGCTTTTCCGC+CGG	+	15173:489-508	MS.gene068179:intergenic	55.0%
	CGACGGTTCCTGGACGGAAA+CGG	+	15173:574-593	MS.gene068179:intergenic	60.0%
	GAAGTGCGACGGTTCCTGGA+CGG	+	15173:580-599	MS.gene068179:intergenic	60.0%
	GGTTCCTGGACGGAAACGGT+GGG	+	15173:570-589	MS.gene068179:intergenic	60.0%
	GTGGGGCTTCTTCACTCCTC+CGG	+	15173:552-571	MS.gene068179:intergenic	60.0%
	GTTCCTGGACGGAAACGGTG+GGG	+	15173:569-588	MS.gene068179:intergenic	60.0%
	TCACGAAGTGCGACGGTTCC+TGG	+	15173:584-603	MS.gene068179:intergenic	60.0%
	TTCTTCACTCCTCCGGTCGC+CGG	+	15173:545-564	MS.gene068179:intergenic	60.0%
	AAGCCCCACCGTTTCCGTCC+AGG	-	15173:563-582	MS.gene068179:CDS	65.0%
	ACCGCCCGAAAATCCACCGG+CGG	-	15173:470-489	MS.gene068179:CDS	65.0%
	AGCCGCTCGGAAATCCGCTC+CGG	-	15173:523-542	MS.gene068179:CDS	65.0%
	CGGTTCCTGGACGGAAACGG+TGG	+	15173:571-590	MS.gene068179:intergenic	65.0%
!!	TCCGCCGGTGGATTTTCGGG+CGG	+	15173:474-493	MS.gene068179:intergenic	65.0%
	AAATCCGCTCCGGCGACCGG+AGG	-	15173:533-552	MS.gene068179:CDS	70.0%
	CGGAAATCCGCTCCGGCGAC+CGG	-	15173:530-549	MS.gene068179:CDS	70.0%
!	CGCCGGAGCGGATTTCCGAG+CGG	+	15173:528-547	MS.gene068179:intergenic	70.0%
!	GCGTGGTGCTTTTCCGCCGG+TGG	+	15173:486-505	MS.gene068179:intergenic	70.0%
	CACTCCTCCGGTCGCCGGAG+CGG	+	15173:540-559	MS.gene068179:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
15173	gene	452	862	452	ID=MS.gene068179
15173	mRNA	452	862	452	ID=MS.gene068179.t1;Parent=MS.gene068179
15173	exon	452	862	452	ID=MS.gene068179.t1.exon1;Parent=MS.gene068179.t1
15173	CDS	452	862	452	ID=cds.MS.gene068179.t1;Parent=MS.gene068179.t1

Gene Sequence

>MS.gene068179

ATGGCACGTACCAAACAAACCGCCCGAAAATCCACCGGCGGAAAAGCACCACGCAAACAACTCGCCACAAAAGCCGCTCGGAAATCCGCTCCGGCGACCGGAGGAGTGAAGAAGCCCCACCGTTTCCGTCCAGGAACCGTCGCACTTCGTGAAATCCGCAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCATTTCAAAGATTGGTTCGAGAAATCGCTCAGGATTTCAAAACGGATCTCCGTTTCCAGAGTAGTGCTGTTTCTGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAAGATACGAATCTTTGTGCTATTCATGCTAAGAGGGTTACTATTATGCCTAAGGATATTCAACTTGCGAGAAGAATCAGAGGCGAAAGGGCTTAG

Protein sequence

>MS.gene068179.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA