AlfalfaGEDB | MS.gene027904

M. sativa cultivar XinJiangDaYe / MS.gene027904

KEGG: K11253
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027904.t1	XP_004512510.2	100	136	0	0	1	136	104	239	5.80E-64	253.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027904.t1	P68429	100.0	136	0	0	1	136	1	136	1.4e-66	253.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027904.t1	A0A1S3TJX1	100.0	136	0	0	1	136	1	136	4.2e-64	253.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene011711	MS.gene027904	PPI
MS.gene027904	MS.gene066536	PPI
MS.gene027904	MS.gene072431	PPI
MS.gene58116	MS.gene027904	PPI
MS.gene027904	MS.gene91631	PPI
MS.gene61192	MS.gene027904	PPI
MS.gene027904	MS.gene03849	PPI
MS.gene027904	MS.gene04748	PPI
MS.gene28432	MS.gene027904	PPI
MS.gene027904	MS.gene50129	PPI
MS.gene027904	MS.gene034255	PPI
MS.gene88233	MS.gene027904	PPI
MS.gene027904	MS.gene49838	PPI
MS.gene97703	MS.gene027904	PPI
MS.gene82109	MS.gene027904	PPI
MS.gene06236	MS.gene027904	PPI
MS.gene95244	MS.gene027904	PPI
MS.gene027904	MS.gene32513	PPI
MS.gene94307	MS.gene027904	PPI
MS.gene027904	MS.gene64164	PPI
MS.gene009155	MS.gene027904	PPI
MS.gene027904	MS.gene41679	PPI
MS.gene78924	MS.gene027904	PPI
MS.gene25357	MS.gene027904	PPI
MS.gene07616	MS.gene027904	PPI
MS.gene027904	MS.gene81646	PPI
MS.gene070538	MS.gene027904	PPI
MS.gene98552	MS.gene027904	PPI
MS.gene027904	MS.gene66752	PPI
MS.gene027904	MS.gene26406	PPI
MS.gene027904	MS.gene70990	PPI
MS.gene027904	MS.gene24196	PPI
MS.gene027904	MS.gene068179	PPI
MS.gene027904	MS.gene027909	PPI
MS.gene027904	MS.gene70261	PPI
MS.gene027904	MS.gene009155	PPI
MS.gene28434	MS.gene027904	PPI
MS.gene016712	MS.gene027904	PPI
MS.gene027904	MS.gene018762	PPI
MS.gene014301	MS.gene027904	PPI
MS.gene000604	MS.gene027904	PPI
MS.gene027904	MS.gene97703	PPI
MS.gene027904	MS.gene64998	PPI
MS.gene030661	MS.gene027904	PPI
MS.gene027904	MS.gene78323	PPI
MS.gene027904	MS.gene58114	PPI
MS.gene027904	MS.gene030664	PPI
MS.gene79799	MS.gene027904	PPI
MS.gene027904	MS.gene66836	PPI
MS.gene059054	MS.gene027904	PPI
MS.gene027904	MS.gene60200	PPI
MS.gene027904	MS.gene55626	PPI
MS.gene027904	MS.gene05905	PPI
MS.gene027904	MS.gene024064	PPI
MS.gene60199	MS.gene027904	PPI
MS.gene021887	MS.gene027904	PPI
MS.gene027904	MS.gene36496	PPI
MS.gene52605	MS.gene027904	PPI
MS.gene013163	MS.gene027904	PPI
MS.gene020313	MS.gene027904	PPI
MS.gene027904	MS.gene057458	PPI
MS.gene027904	MS.gene29521	PPI
MS.gene32514	MS.gene027904	PPI
MS.gene064876	MS.gene027904	PPI
MS.gene44458	MS.gene027904	PPI
MS.gene028296	MS.gene027904	PPI
MS.gene39538	MS.gene027904	PPI
MS.gene027904	MS.gene056335	PPI
MS.gene027904	MS.gene58112	PPI
MS.gene027904	MS.gene29300	PPI
MS.gene042871	MS.gene027904	PPI
MS.gene78922	MS.gene027904	PPI
MS.gene071851	MS.gene027904	PPI
MS.gene027904	MS.gene072432	PPI
MS.gene030666	MS.gene027904	PPI
MS.gene027904	MS.gene89203	PPI
MS.gene027904	MS.gene00233	PPI
MS.gene027904	MS.gene63118	PPI
MS.gene027904	MS.gene68716	PPI
MS.gene97701	MS.gene027904	PPI
MS.gene027904	MS.gene068178	PPI
MS.gene027904	MS.gene47573	PPI
MS.gene027904	MS.gene94315	PPI
MS.gene027904	MS.gene059054	PPI
MS.gene027904	MS.gene47284	PPI
MS.gene014181	MS.gene027904	PPI
MS.gene027904	MS.gene60196	PPI
MS.gene027904	MS.gene61193	PPI
MS.gene027904	MS.gene069975	PPI
MS.gene027904	MS.gene34797	PPI
MS.gene027904	MS.gene78324	PPI
MS.gene068178	MS.gene027904	PPI
MS.gene44457	MS.gene027904	PPI
MS.gene027904	MS.gene26084	PPI
MS.gene68113	MS.gene027904	PPI
MS.gene053230	MS.gene027904	PPI
MS.gene027904	MS.gene017431	PPI
MS.gene027904	MS.gene78227	PPI
MS.gene027904	MS.gene70258	PPI
MS.gene66051	MS.gene027904	PPI
MS.gene027904	MS.gene066537	PPI
MS.gene027904	MS.gene65125	PPI
MS.gene87898	MS.gene027904	PPI
MS.gene027904	MS.gene011710	PPI
MS.gene04820	MS.gene027904	PPI
MS.gene072433	MS.gene027904	PPI
MS.gene027904	MS.gene23886	PPI
MS.gene027904	MS.gene29299	PPI
MS.gene027904	MS.gene015760	PPI
MS.gene046235	MS.gene027904	PPI
MS.gene027904	MS.gene35163	PPI
MS.gene027904	MS.gene066535	PPI
MS.gene027904	MS.gene29445	PPI
MS.gene027904	MS.gene78224	PPI
MS.gene027904	MS.gene038612	PPI
MS.gene055512	MS.gene027904	PPI
MS.gene027904	MS.gene033505	PPI
MS.gene049111	MS.gene027904	PPI
MS.gene70263	MS.gene027904	PPI
MS.gene58285	MS.gene027904	PPI
MS.gene027904	MS.gene29298	PPI
MS.gene027904	MS.gene46303	PPI
MS.gene027904	MS.gene28438	PPI
MS.gene027904	MS.gene64996	PPI
MS.gene027907	MS.gene027904	PPI
MS.gene027904	MS.gene066960	PPI
MS.gene067742	MS.gene027904	PPI
MS.gene027904	MS.gene063067	PPI
MS.gene78322	MS.gene027904	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027904.t1	MTR_7g059070	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_5g029820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_5g029770	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_4g065990	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_4g088150	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_2g035230	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_8g092720	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_8g063500	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_8g103245	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_8g092820	100.000	136	0	0	1	136	1	136	4.79e-96	272
MS.gene027904.t1	MTR_7g013610	100.000	136	0	0	1	136	100	235	1.10e-95	275
MS.gene027904.t1	MTR_4g097175	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene027904.t1	MTR_1g023630	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene027904.t1	MTR_8g061940	97.059	136	4	0	1	136	1	136	3.29e-93	265
MS.gene027904.t1	MTR_8g099430	96.324	136	5	0	1	136	1	136	1.38e-92	264
MS.gene027904.t1	MTR_4g097170	96.324	136	5	0	1	136	42	177	2.29e-91	262
MS.gene027904.t1	MTR_2g082310	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene027904.t1	MTR_2g082340	89.706	136	14	0	1	136	1	136	1.38e-86	248
MS.gene027904.t1	MTR_2g082370	85.616	146	11	1	1	136	1	146	1.74e-82	238
MS.gene027904.t1	MTR_2g082400	84.559	136	19	2	1	135	1	135	6.38e-75	219
MS.gene027904.t1	MTR_7g013600	86.607	112	15	0	25	136	2	113	1.01e-65	195
MS.gene027904.t1	MTR_7g023830	67.164	134	43	1	1	134	1	133	6.48e-56	171
MS.gene027904.t1	MTR_1g023630	73.504	117	15	3	1	111	1	107	3.81e-52	160
MS.gene027904.t1	MTR_5g024630	67.241	116	35	2	1	116	1	113	1.63e-45	144
MS.gene027904.t1	MTR_7g023880	58.647	133	41	3	3	134	18	137	5.46e-43	139
MS.gene027904.t1	MTR_5g024530	68.182	110	12	2	19	128	68	154	4.56e-42	137
MS.gene027904.t1	MTR_0197s0100	100.000	66	0	0	1	66	1	66	1.45e-40	132
MS.gene027904.t1	MTR_8g027840	55.102	98	43	1	37	133	28	125	4.83e-30	105
MS.gene027904.t1	MTR_8g105150	53.846	91	10	1	37	127	11	69	1.43e-25	92.4
MS.gene027904.t1	MTR_8g092740	97.368	38	1	0	99	136	118	155	4.60e-20	80.9
MS.gene027904.t1	MTR_5g089120	76.190	42	10	0	82	123	6	47	6.07e-18	73.6
MS.gene027904.t1	MTR_5g024600	62.963	54	20	0	1	54	1	54	1.76e-15	66.2
MS.gene027904.t1	MTR_4g128040	75.676	37	9	0	79	115	20	56	3.38e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027904.t1	AT5G65360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene027904.t1	AT5G10390	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene027904.t1	AT5G10400	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene027904.t1	AT3G27360	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene027904.t1	AT1G09200	99.265	136	1	0	1	136	1	136	1.55e-95	271
MS.gene027904.t1	AT5G10980	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40040	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40030	97.059	136	4	0	1	136	1	136	3.44e-93	265
MS.gene027904.t1	AT4G40030	97.059	136	4	0	1	136	29	164	6.76e-93	266
MS.gene027904.t1	AT5G65350	94.853	136	7	0	1	136	1	136	5.09e-91	260
MS.gene027904.t1	AT1G75600	93.382	136	9	0	1	136	1	136	1.20e-89	256
MS.gene027904.t1	AT1G13370	93.382	136	9	0	1	136	1	136	5.32e-89	254
MS.gene027904.t1	AT1G19890	89.781	137	13	1	1	136	1	137	1.03e-83	241
MS.gene027904.t1	AT5G12910	71.111	135	34	2	1	135	1	130	9.51e-67	198
MS.gene027904.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene027904.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene027904.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110
MS.gene027904.t1	AT1G01370	53.982	113	49	2	24	134	63	174	2.86e-31	110

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTGACTCCACCCGTTGC+CGG	0.305872	5.2:+18712831	None:intergenic
TGAGAAGCTCGGTGCTCTTC+TGG	0.307615	5.2:+18712757	None:intergenic
AGCAGATTTGCGAGCAGCTT+TGG	0.335283	5.2:+18712854	None:intergenic
CGCAAATCTGCTCCGGCAAC+GGG	0.354795	5.2:-18712843	MS.gene027904:CDS
GCTGCTGAAGCTTATCTTGT+TGG	0.357332	5.2:-18712636	MS.gene027904:CDS
TCACGAAGTGCAACAGTTCC+AGG	0.377831	5.2:+18712792	None:intergenic
TCGCAAATCTGCTCCGGCAA+CGG	0.410102	5.2:-18712844	MS.gene027904:CDS
TTGGTGGCTAGCTGCTTCCT+TGG	0.412728	5.2:+18712873	None:intergenic
GAGCGGAAACAGCACTGCTT+TGG	0.422995	5.2:+18712667	None:intergenic
TTATCTTGTTGGTTTGTTTG+AGG	0.428809	5.2:-18712625	MS.gene027904:CDS
CGATTTCACGAACAAGTCTC+TGG	0.432729	5.2:+18712718	None:intergenic
AAACAGCACTGCTTTGGAAT+CGG	0.472990	5.2:+18712673	None:intergenic
CGAGCGAGTTGAATATCCTT+AGG	0.499677	5.2:+18712558	None:intergenic
AGATTTGCGAGCAGCTTTGG+TGG	0.517744	5.2:+18712857	None:intergenic
AGCTGCTCGCAAATCTGCTC+CGG	0.521796	5.2:-18712850	MS.gene027904:CDS
AGAGCACCGAGCTTCTCATA+AGG	0.522726	5.2:-18712752	MS.gene027904:CDS
GGATTTGCGAGCAGTTTGCT+TGG	0.522741	5.2:+18712911	None:intergenic
AGTTTGCTTGGTACGAGCCA+TGG	0.544971	5.2:+18712923	None:intergenic
GAGAGTTACTATCATGCCTA+AGG	0.550012	5.2:-18712574	MS.gene027904:CDS
ACTTGTTCGTGAAATCGCTC+AGG	0.551065	5.2:-18712712	MS.gene027904:CDS
CAACTCGCTCGCAGAATCAG+AGG	0.556058	5.2:-18712546	MS.gene027904:CDS
CGGTGCTCTTCTGGTACTTG+CGG	0.565845	5.2:+18712766	None:intergenic
CAAACTGCTCGCAAATCCAC+CGG	0.571388	5.2:-18712906	MS.gene027904:CDS
TTCAGCAGCTTCTTGAAGAG+CGG	0.576635	5.2:+18712650	None:intergenic
TCACGAACAAGTCTCTGGAA+TGG	0.590006	5.2:+18712723	None:intergenic
AAGTTTCCTTATGAGAAGCT+CGG	0.601334	5.2:+18712746	None:intergenic
AAATCTGCTCCGGCAACGGG+TGG	0.604476	5.2:-18712840	MS.gene027904:CDS
AAGCCACACAGATTCCGTCC+TGG	0.622196	5.2:-18712810	MS.gene027904:CDS
TCTTAGCATGAATAGCGCAA+AGG	0.644849	5.2:+18712595	None:intergenic
CCACCGGCGGAAAAGCACCA+AGG	0.683879	5.2:-18712890	MS.gene027904:CDS
ACTGCTCGCAAATCCACCGG+CGG	0.685762	5.2:-18712903	MS.gene027904:CDS
GAAGTGCAACAGTTCCAGGA+CGG	0.690156	5.2:+18712796	None:intergenic
GTTCCAGGACGGAATCTGTG+TGG	0.699556	5.2:+18712807	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATCTTGTTGGTTTGTTTG+AGG	-	chr5.2:18712829-18712848	MS.gene027904:CDS	30.0%
	AAGTTTCCTTATGAGAAGCT+CGG	+	chr5.2:18712711-18712730	None:intergenic	35.0%
	GAGAGTTACTATCATGCCTA+AGG	-	chr5.2:18712880-18712899	MS.gene027904:CDS	40.0%
	TCTTAGCATGAATAGCGCAA+AGG	+	chr5.2:18712862-18712881	None:intergenic	40.0%
!!	AAACAGCACTGCTTTGGAAT+CGG	+	chr5.2:18712784-18712803	None:intergenic	40.0%
	ACTTGTTCGTGAAATCGCTC+AGG	-	chr5.2:18712742-18712761	MS.gene027904:CDS	45.0%
	CGATTTCACGAACAAGTCTC+TGG	+	chr5.2:18712739-18712758	None:intergenic	45.0%
	GCTGCTGAAGCTTATCTTGT+TGG	-	chr5.2:18712818-18712837	MS.gene027904:CDS	45.0%
	TTCAGCAGCTTCTTGAAGAG+CGG	+	chr5.2:18712807-18712826	None:intergenic	45.0%
!	CGAGCGAGTTGAATATCCTT+AGG	+	chr5.2:18712899-18712918	None:intergenic	45.0%
!	TCACGAACAAGTCTCTGGAA+TGG	+	chr5.2:18712734-18712753	None:intergenic	45.0%
	AGCAGATTTGCGAGCAGCTT+TGG	+	chr5.2:18712603-18712622	None:intergenic	50.0%
	CAAACTGCTCGCAAATCCAC+CGG	-	chr5.2:18712548-18712567	MS.gene027904:CDS	50.0%
	GAAGTGCAACAGTTCCAGGA+CGG	+	chr5.2:18712661-18712680	None:intergenic	50.0%
	TCACGAAGTGCAACAGTTCC+AGG	+	chr5.2:18712665-18712684	None:intergenic	50.0%
!	AGATTTGCGAGCAGCTTTGG+TGG	+	chr5.2:18712600-18712619	None:intergenic	50.0%
!	GGATTTGCGAGCAGTTTGCT+TGG	+	chr5.2:18712546-18712565	None:intergenic	50.0%
!!	AGAGCACCGAGCTTCTCATA+AGG	-	chr5.2:18712702-18712721	MS.gene027904:CDS	50.0%
	AAGCCACACAGATTCCGTCC+TGG	-	chr5.2:18712644-18712663	MS.gene027904:CDS	55.0%
	AGCTGCTCGCAAATCTGCTC+CGG	-	chr5.2:18712604-18712623	MS.gene027904:CDS	55.0%
	CAACTCGCTCGCAGAATCAG+AGG	-	chr5.2:18712908-18712927	MS.gene027904:CDS	55.0%
	CGGTGCTCTTCTGGTACTTG+CGG	+	chr5.2:18712691-18712710	None:intergenic	55.0%
	GAGCGGAAACAGCACTGCTT+TGG	+	chr5.2:18712790-18712809	None:intergenic	55.0%
	GTTCCAGGACGGAATCTGTG+TGG	+	chr5.2:18712650-18712669	None:intergenic	55.0%
	TCGCAAATCTGCTCCGGCAA+CGG	-	chr5.2:18712610-18712629	MS.gene027904:CDS	55.0%
	TTCTTGACTCCACCCGTTGC+CGG	+	chr5.2:18712626-18712645	None:intergenic	55.0%
!	TTGGTGGCTAGCTGCTTCCT+TGG	+	chr5.2:18712584-18712603	None:intergenic	55.0%
!!	TGAGAAGCTCGGTGCTCTTC+TGG	+	chr5.2:18712700-18712719	None:intergenic	55.0%
!!!	CTTCCTTGGTGCTTTTCCGC+CGG	+	chr5.2:18712570-18712589	None:intergenic	55.0%
	AAATCTGCTCCGGCAACGGG+TGG	-	chr5.2:18712614-18712633	MS.gene027904:CDS	60.0%
	ACTGCTCGCAAATCCACCGG+CGG	-	chr5.2:18712551-18712570	MS.gene027904:CDS	60.0%
	CGCAAATCTGCTCCGGCAAC+GGG	-	chr5.2:18712611-18712630	MS.gene027904:CDS	60.0%
!!	CCACCGGCGGAAAAGCACCA+AGG	-	chr5.2:18712564-18712583	MS.gene027904:CDS	65.0%
!!!	CCTTGGTGCTTTTCCGCCGG+TGG	+	chr5.2:18712567-18712586	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	18712533	18712943	18712533	ID=MS.gene027904
chr5.2	mRNA	18712533	18712943	18712533	ID=MS.gene027904.t1;Parent=MS.gene027904
chr5.2	exon	18712533	18712943	18712533	ID=MS.gene027904.t1.exon1;Parent=MS.gene027904.t1
chr5.2	CDS	18712533	18712943	18712533	ID=cds.MS.gene027904.t1;Parent=MS.gene027904.t1

Gene Sequence

>MS.gene027904

ATGGCTCGTACCAAGCAAACTGCTCGCAAATCCACCGGCGGAAAAGCACCAAGGAAGCAGCTAGCCACCAAAGCTGCTCGCAAATCTGCTCCGGCAACGGGTGGAGTCAAGAAGCCACACAGATTCCGTCCTGGAACTGTTGCACTTCGTGAGATCCGCAAGTACCAGAAGAGCACCGAGCTTCTCATAAGGAAACTTCCATTCCAGAGACTTGTTCGTGAAATCGCTCAGGATTTCAAAACAGATCTCCGATTCCAAAGCAGTGCTGTTTCCGCTCTTCAAGAAGCTGCTGAAGCTTATCTTGTTGGTTTGTTTGAGGATACTAACCTTTGCGCTATTCATGCTAAGAGAGTTACTATCATGCCTAAGGATATTCAACTCGCTCGCAGAATCAGAGGCGAGAGAGCTTAG

Protein sequence

>MS.gene027904.t1

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA