AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92401


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92401.t1 MTR_8g037933 94.054 185 5 1 1 185 1 179 8.77e-115 323
MS.gene92401.t1 MTR_0695s0010 88.889 36 4 0 35 70 18 53 8.57e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCATTATGTCCTTCT+AGG 0.088496 8.4:-60297820 None:intergenic
TTTGGAACAGGCTTGTAAAT+TGG 0.196645 8.4:-60298061 None:intergenic
TTTGGAATAGGTTTGTAAAT+TGG 0.202275 8.4:-60298157 None:intergenic
CTTTGGAATAGGCTTGTAAA+TGG 0.212935 8.4:-60298014 None:intergenic
TTATAGACTGGAACGTATTT+TGG 0.231955 8.4:-60297971 None:intergenic
TTTGGAATAGGCTTGTAAAT+TGG 0.232414 8.4:-60298109 None:intergenic
GGAATTGGTTTCTTGAATAT+TGG 0.237384 8.4:-60297902 None:intergenic
TTGTGAATAGGTTTATAAAA+TGG 0.240012 8.4:-60297923 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.4:-60298043 None:intergenic
ATTGGAACAGGCTTGTAAAT+TGG 0.276520 8.4:-60298091 None:intergenic
TTTGGTATAGGTTTGTAAAT+TGG 0.283056 8.4:-60297953 None:intergenic
ATTGGAATAGGCTTGTAAAT+TGG 0.290762 8.4:-60298139 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.4:-60298025 None:intergenic
ATTGGAATTACCTTTGGAAT+AGG 0.316743 8.4:-60298121 None:intergenic
ATTGGAATGTCTTTGTGAAT+AGG 0.322130 8.4:-60297935 None:intergenic
CAAAAGTGGCCTTTGCAAAT+TGG 0.325296 8.4:-60296897 None:intergenic
CAATTTGCAAAGGCCACTTT+TGG 0.327118 8.4:+60296898 MS.gene92401:CDS
ATAGGTTTGTAAATTGGAAT+AGG 0.345184 8.4:-60298151 None:intergenic
ATGGGAATTTCCTTTGGAAT+AGG 0.348694 8.4:-60298169 None:intergenic
ATAGGCTTGTAAATGGGAAT+TGG 0.370906 8.4:-60298007 None:intergenic
TTTACAATGGGAATTTCCTT+TGG 0.389183 8.4:-60298175 None:intergenic
GTAGCAAGAAATGATCCTTC+AGG 0.392442 8.4:+60296974 MS.gene92401:CDS
ATAGGTTTATAAAATGGAAT+TGG 0.398934 8.4:-60297917 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.4:-60298031 None:intergenic
TTGTAAATTGGAATTACCTT+TGG 0.416670 8.4:-60298127 None:intergenic
TTGTAAATTGGAATAACCTT+TGG 0.416881 8.4:-60298079 None:intergenic
ATTGGAATAACCTTTGGAAC+AGG 0.418441 8.4:-60298073 None:intergenic
TAATGGATTGGCTTTACAAT+GGG 0.423378 8.4:-60298187 None:intergenic
ATAGGCTTGTAAATTGGAAC+AGG 0.440807 8.4:-60298103 None:intergenic
ACAGGCTTGTAAATTGGAAC+AGG 0.447839 8.4:-60298055 None:intergenic
ATGGGAATTGGTTTGTGGAT+AGG 0.448083 8.4:-60297995 None:intergenic
GCTTCCCATCTGTGTTCACT+TGG 0.449323 8.4:-60297769 None:intergenic
GCATGATAATCTTAATGGAT+TGG 0.454921 8.4:-60298199 None:intergenic
GCAACAAAGACAAACCTAGA+AGG 0.464842 8.4:+60297806 MS.gene92401:CDS
TGTAAATGGGAATTGGTTTG+TGG 0.491139 8.4:-60298000 None:intergenic
TTTGGAATAGGCTTGTAAAT+GGG 0.493642 8.4:-60298013 None:intergenic
AACAGAAGTAGCATAACAAA+AGG 0.510420 8.4:-60296951 None:intergenic
TTAATGGATTGGCTTTACAA+TGG 0.537191 8.4:-60298188 None:intergenic
TTGTGGATAGGCTTATAGAC+TGG 0.538000 8.4:-60297983 None:intergenic
GGAAACCAAGTGAACACAGA+TGG 0.546430 8.4:+60297764 MS.gene92401:intron
CATCTAAAACACAAACTCAA+AGG 0.559830 8.4:+60297863 MS.gene92401:CDS
AATTTACAAACCTATTCCAA+AGG 0.560996 8.4:+60298159 MS.gene92401:CDS
AATTTACAAGCCTGTTCCAA+AGG 0.571317 8.4:+60298063 MS.gene92401:CDS
CATTTACAAGCCTATTCCAA+AGG 0.578514 8.4:+60298015 MS.gene92401:CDS
GAAACCAAGTGAACACAGAT+GGG 0.582541 8.4:+60297765 MS.gene92401:CDS
GGAACCCAGAAGGCCAAAAG+TGG 0.583318 8.4:-60296911 None:intergenic
AATTTACAAGCCTATTCCAA+AGG 0.584966 8.4:+60298111 MS.gene92401:CDS
GAAAAGCTTCCAATTTGCAA+AGG 0.597703 8.4:+60296888 MS.gene92401:CDS
AAGGTGACAAGGAGCAACAA+AGG 0.600910 8.4:-60296932 None:intergenic
TGGGAAGCTTGCAAATGAAG+AGG 0.610090 8.4:+60297784 MS.gene92401:CDS
GAGCAACAAAGGAACCCAGA+AGG 0.618628 8.4:-60296921 None:intergenic
TAGCATAACAAAAGGTGACA+AGG 0.637763 8.4:-60296943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATAAAATTTTATTTGA+AGG + chr8.4:60297404-60297423 MS.gene92401:intron 10.0%
!! AAAAAAGAGAGAGAAATTTA+TGG + chr8.4:60297149-60297168 MS.gene92401:intron 20.0%
!! AAACAAAACAAACATAAACT+TGG + chr8.4:60297685-60297704 MS.gene92401:intron 20.0%
!! AATGAAGAAGAAAAATTCAA+AGG + chr8.4:60297833-60297852 MS.gene92401:CDS 20.0%
!! ACATTTATATTTCCCTTAAT+TGG + chr8.4:60297125-60297144 MS.gene92401:intron 20.0%
!! ATAGGTTTATAAAATGGAAT+TGG - chr8.4:60297920-60297939 None:intergenic 20.0%
!! GTTCATAAAATATTGTTTGT+TGG + chr8.4:60297205-60297224 MS.gene92401:intron 20.0%
!! TTCTATGACTAATTTCTAAT+AGG - chr8.4:60297627-60297646 None:intergenic 20.0%
!! TTGTGAATAGGTTTATAAAA+TGG - chr8.4:60297926-60297945 None:intergenic 20.0%
! AATTTACAAACCTATTCCAA+AGG + chr8.4:60298159-60298178 MS.gene92401:CDS 25.0%
! ACCATTTAAACATGACTTTA+AGG - chr8.4:60297261-60297280 None:intergenic 25.0%
! ATAGGTTTGTAAATTGGAAT+AGG - chr8.4:60297950-60297969 None:intergenic 25.0%
! TCCTTAAAGTCATGTTTAAA+TGG + chr8.4:60297257-60297276 MS.gene92401:intron 25.0%
! TTGTAAATTGGAATAACCTT+TGG - chr8.4:60298082-60298101 None:intergenic 25.0%
! TTGTAAATTGGAATTACCTT+TGG - chr8.4:60298034-60298053 None:intergenic 25.0%
! TTTGGTATAGGTTTGTAAAT+TGG - chr8.4:60297956-60297975 None:intergenic 25.0%
!! TTTGGAATAGGTTTGTAAAT+TGG - chr8.4:60298160-60298179 None:intergenic 25.0%
!!! CTCTCTTTTTTACCAATTAA+GGG - chr8.4:60297140-60297159 None:intergenic 25.0%
!!! GTTTCTTGAATATTGGTTTT+TGG - chr8.4:60297898-60297917 None:intergenic 25.0%
!!! TCTCTCTTTTTTACCAATTA+AGG - chr8.4:60297141-60297160 None:intergenic 25.0%
AACAGAAGTAGCATAACAAA+AGG - chr8.4:60296954-60296973 None:intergenic 30.0%
AATTTACAAGCCTATTCCAA+AGG + chr8.4:60298111-60298130 MS.gene92401:CDS 30.0%
ACTATCATCATAATCACACA+AGG - chr8.4:60297305-60297324 None:intergenic 30.0%
AGTAGTACATATTCATAGAC+TGG + chr8.4:60297075-60297094 MS.gene92401:intron 30.0%
ATTGGAATAGGCTTGTAAAT+TGG - chr8.4:60298142-60298161 None:intergenic 30.0%
ATTGGAATGTCTTTGTGAAT+AGG - chr8.4:60297938-60297957 None:intergenic 30.0%
GTAGTACATATTCATAGACT+GGG + chr8.4:60297076-60297095 MS.gene92401:intron 30.0%
TTGCTCAAGTTATAAACTCT+AGG - chr8.4:60297726-60297745 None:intergenic 30.0%
TTTACAATGGGAATTTCCTT+TGG - chr8.4:60298178-60298197 None:intergenic 30.0%
! CATCTAAAACACAAACTCAA+AGG + chr8.4:60297863-60297882 MS.gene92401:CDS 30.0%
! GGAATTGGTTTCTTGAATAT+TGG - chr8.4:60297905-60297924 None:intergenic 30.0%
! TTATAGACTGGAACGTATTT+TGG - chr8.4:60297974-60297993 None:intergenic 30.0%
! TTTGGAATAGGCTTGTAAAT+GGG - chr8.4:60298016-60298035 None:intergenic 30.0%
! TTTGGAATAGGCTTGTAAAT+TGG - chr8.4:60298016-60298035 None:intergenic 30.0%
!! ATTGGAATTACCTTTGGAAT+AGG - chr8.4:60298028-60298047 None:intergenic 30.0%
!! TAATGGATTGGCTTTACAAT+GGG - chr8.4:60298190-60298209 None:intergenic 30.0%
!! TTAATGGATTGGCTTTACAA+TGG - chr8.4:60298191-60298210 None:intergenic 30.0%
!!! GGGTTATTTTTGTTTACTCT+TGG + chr8.4:60297096-60297115 MS.gene92401:intron 30.0%
AATTTACAAGCCTGTTCCAA+AGG + chr8.4:60298045-60298064 MS.gene92401:CDS 35.0%
ATAAACTTGGTCATTGAGCT+AGG + chr8.4:60297698-60297717 MS.gene92401:intron 35.0%
ATAGGCTTGTAAATGGGAAT+TGG - chr8.4:60298010-60298029 None:intergenic 35.0%
ATAGGCTTGTAAATTGGAAC+AGG - chr8.4:60298106-60298125 None:intergenic 35.0%
ATTCAAACCAATACACACAC+AGG + chr8.4:60297505-60297524 MS.gene92401:intron 35.0%
ATTGGAACAGGCTTGTAAAT+TGG - chr8.4:60298046-60298065 None:intergenic 35.0%
CATTTACAAGCCTATTCCAA+AGG + chr8.4:60298015-60298034 MS.gene92401:CDS 35.0%
CTTCTTCATTATGTCCTTCT+AGG - chr8.4:60297823-60297842 None:intergenic 35.0%
GAAAAGCTTCCAATTTGCAA+AGG + chr8.4:60296888-60296907 MS.gene92401:CDS 35.0%
GGTTCAAGAAGAATTGAACT+CGG + chr8.4:60297537-60297556 MS.gene92401:intron 35.0%
TAGCATAACAAAAGGTGACA+AGG - chr8.4:60296946-60296965 None:intergenic 35.0%
TATTGTACTCACAAACCTGA+AGG - chr8.4:60296992-60297011 None:intergenic 35.0%
! CAATTTTGCATTTCTTGTGC+AGG + chr8.4:60297743-60297762 MS.gene92401:intron 35.0%
! TTTGGAACAGGCTTGTAAAT+TGG - chr8.4:60298064-60298083 None:intergenic 35.0%
!! ATGGGAATTTCCTTTGGAAT+AGG - chr8.4:60298172-60298191 None:intergenic 35.0%
!! ATTGGAATAACCTTTGGAAC+AGG - chr8.4:60298076-60298095 None:intergenic 35.0%
!! CTTTGGAATAGGCTTGTAAA+TGG - chr8.4:60298017-60298036 None:intergenic 35.0%
!! TGTAAATGGGAATTGGTTTG+TGG - chr8.4:60298003-60298022 None:intergenic 35.0%
!!! ACTGGAACGTATTTTGGTAT+AGG - chr8.4:60297968-60297987 None:intergenic 35.0%
ACAGGCTTGTAAATTGGAAC+AGG - chr8.4:60298058-60298077 None:intergenic 40.0%
CAAAAGTGGCCTTTGCAAAT+TGG - chr8.4:60296900-60296919 None:intergenic 40.0%
GAAACCAAGTGAACACAGAT+GGG + chr8.4:60297765-60297784 MS.gene92401:CDS 40.0%
GCAACAAAGACAAACCTAGA+AGG + chr8.4:60297806-60297825 MS.gene92401:CDS 40.0%
GTAGCAAGAAATGATCCTTC+AGG + chr8.4:60296974-60296993 MS.gene92401:CDS 40.0%
TTGTGGATAGGCTTATAGAC+TGG - chr8.4:60297986-60298005 None:intergenic 40.0%
!! ATGGGAATTGGTTTGTGGAT+AGG - chr8.4:60297998-60298017 None:intergenic 40.0%
!! CAATTTGCAAAGGCCACTTT+TGG + chr8.4:60296898-60296917 MS.gene92401:CDS 40.0%
AAGGTGACAAGGAGCAACAA+AGG - chr8.4:60296935-60296954 None:intergenic 45.0%
GGAAACCAAGTGAACACAGA+TGG + chr8.4:60297764-60297783 MS.gene92401:intron 45.0%
TGGGAAGCTTGCAAATGAAG+AGG + chr8.4:60297784-60297803 MS.gene92401:CDS 45.0%
!!! ATAATTTAGTTTTTAATAAA+AGG - chr8.4:60297454-60297473 None:intergenic 5.0%
CGTAGTGCCTGTGTGTGTAT+TGG - chr8.4:60297515-60297534 None:intergenic 50.0%
GAGCAACAAAGGAACCCAGA+AGG - chr8.4:60296924-60296943 None:intergenic 50.0%
GCTTCCCATCTGTGTTCACT+TGG - chr8.4:60297772-60297791 None:intergenic 50.0%
TACACACACAGGCACTACGT+TGG + chr8.4:60297516-60297535 MS.gene92401:intron 50.0%
!!! AAAGGCCACTTTTGGCCTTC+TGG + chr8.4:60296906-60296925 MS.gene92401:CDS 50.0%
!!! AAGGCCACTTTTGGCCTTCT+GGG + chr8.4:60296907-60296926 MS.gene92401:CDS 50.0%
GGAACCCAGAAGGCCAAAAG+TGG - chr8.4:60296914-60296933 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 60296884 60298210 60296884 ID=MS.gene92401
chr8.4 mRNA 60296884 60298210 60296884 ID=MS.gene92401.t1;Parent=MS.gene92401
chr8.4 exon 60296884 60296995 60296884 ID=MS.gene92401.t1.exon1;Parent=MS.gene92401.t1
chr8.4 CDS 60296884 60296995 60296884 ID=cds.MS.gene92401.t1;Parent=MS.gene92401.t1
chr8.4 exon 60297765 60298210 60297765 ID=MS.gene92401.t1.exon2;Parent=MS.gene92401.t1
chr8.4 CDS 60297765 60298210 60297765 ID=cds.MS.gene92401.t1;Parent=MS.gene92401.t1
Gene Sequence

>MS.gene92401

ATGAGAAAAGCTTCCAATTTGCAAAGGCCACTTTTGGCCTTCTGGGTTCCTTTGTTGCTCCTTGTCACCTTTTGTTATGCTACTTCTGTTGTAGCAAGAAATGATCCTTCAGGTTTGTGAGTACAATATTCATTTTGATATTGATGACATATAAATGTACAATATATGTACAGAATATATATAGTAGTAGTAGTAGTACATATTCATAGACTGGGTTATTTTTGTTTACTCTTGGTATAGTACATTTATATTTCCCTTAATTGGTAAAAAAGAGAGAGAAATTTATGGTGTTAAAAATGACTTGAAATTGAAAATATCATTGTTCATAAAATATTGTTTGTTGGTAGTAGTATGTTTGATGAAAAAGTTTAGCTCCTTAAAGTCATGTTTAAATGGTCTTTTTTTAACTTCACTTTTTCCTTGTGTGATTATGATGATAGTAACGTAAAATAAAATTTAATTTTAAAAGCATGTTTAATTAAAAGTGAAAACATATAGTTTTTGTATTGAGCAGAAAATTTTGAATAAAATTTTATTTGAAGGTGATATTTCTTTTATTGTAATTCTCCTTTTATTAAAAACTAAATTATTTGTAAATTAAGAAACGAATTAATAGTATACATTCAAACCAATACACACACAGGCACTACGTTGGTTCAAGAAGAATTGAACTCGGATCTCTTAAATAAATCGAGCATTGTGAATGAAAAAAATTATAAACTCAATAATCAGTCAAATTTCCTATTAGAAATTAGTCATAGAAAAGTTGATAGATATTTCGCACAAATAAGATTGTTACAAAAACAAAACAAACATAAACTTGGTCATTGAGCTAGGAACCTAGAGTTTATAACTTGAGCAATTTTGCATTTCTTGTGCAGGAAACCAAGTGAACACAGATGGGAAGCTTGCAAATGAAGAGGCAACAAAGACAAACCTAGAAGGACATAATGAAGAAGAAAAATTCAAAGGATTTTTTCATCTAAAACACAAACTCAAAGGATATTTTCACCAAAAACCAATATTCAAGAAACCAATTCCATTTTATAAACCTATTCACAAAGACATTCCAATTTACAAACCTATACCAAAATACGTTCCAGTCTATAAGCCTATCCACAAACCAATTCCCATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCTGTTCCAAAGGTTATTCCAATTTACAAGCCTGTTCCAATTTACAAGCCTATTCCAAAGGTAATTCCAATTTACAAGCCTATTCCAATTTACAAACCTATTCCAAAGGAAATTCCCATTGTAAAGCCAATCCATTAA

Protein sequence

>MS.gene92401.t1

MRKASNLQRPLLAFWVPLLLLVTFCYATSVVARNDPSGNQVNTDGKLANEEATKTNLEGHNEEEKFKGFFHLKHKLKGYFHQKPIFKKPIPFYKPIHKDIPIYKPIPKYVPVYKPIHKPIPIYKPIPKVIPIYKPVPIYKPVPKVIPIYKPVPIYKPIPKVIPIYKPIPIYKPIPKEIPIVKPIH