Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048065.01 | MsG0480021189.01 | 0.808321 | 3.279987e-50 | 1.869760e-47 |
| MsG0080048660.01 | MsG0480021189.01 | 0.817824 | 2.699043e-52 | 1.986512e-49 |
| MsG0080048826.01 | MsG0480021189.01 | 0.819946 | 8.890546e-53 | 6.939313e-50 |
| MsG0180000470.01 | MsG0480021189.01 | 0.833511 | 5.113461e-56 | 5.901919e-53 |
| MsG0180001063.01 | MsG0480021189.01 | 0.807267 | 5.494276e-50 | 3.046745e-47 |
| MsG0180001892.01 | MsG0480021189.01 | 0.809563 | 1.778432e-50 | 1.047676e-47 |
| MsG0180002794.01 | MsG0480021189.01 | 0.804220 | 2.398509e-49 | 1.229713e-46 |
| MsG0180004389.01 | MsG0480021189.01 | 0.821288 | 4.371747e-53 | 3.541820e-50 |
| MsG0180004465.01 | MsG0480021189.01 | 0.801486 | 8.802315e-49 | 4.208798e-46 |
| MsG0180004765.01 | MsG0480021189.01 | 0.814152 | 1.781723e-51 | 1.186286e-48 |
| MsG0180004926.01 | MsG0480021189.01 | 0.807241 | 5.564288e-50 | 3.083458e-47 |
| MsG0180004928.01 | MsG0480021189.01 | 0.825654 | 4.166302e-54 | 3.821027e-51 |
| MsG0180005153.01 | MsG0480021189.01 | 0.802992 | 4.310922e-49 | 2.141635e-46 |
| MsG0280006306.01 | MsG0480021189.01 | -0.805885 | 1.075606e-49 | 5.756314e-47 |
| MsG0280006309.01 | MsG0480021189.01 | 0.824810 | 6.595453e-54 | 5.905393e-51 |
| MsG0280006324.01 | MsG0480021189.01 | 0.808538 | 2.948992e-50 | 1.690555e-47 |
| MsG0280006405.01 | MsG0480021189.01 | 0.811319 | 7.430056e-51 | 4.584634e-48 |
| MsG0280010722.01 | MsG0480021189.01 | 0.837556 | 4.846907e-57 | 6.318926e-54 |
| MsG0280010992.01 | MsG0480021189.01 | 0.804252 | 2.361764e-49 | 1.211869e-46 |
| MsG0280011101.01 | MsG0480021189.01 | 0.800479 | 1.413573e-48 | 6.588684e-46 |
| MsG0280011339.01 | MsG0480021189.01 | 0.819441 | 1.159513e-52 | 8.923550e-50 |
| MsG0380011609.01 | MsG0480021189.01 | 0.848256 | 6.880276e-60 | 1.255152e-56 |
| MsG0380013183.01 | MsG0480021189.01 | 0.820156 | 7.959804e-53 | 6.249473e-50 |
| MsG0380014225.01 | MsG0480021189.01 | -0.805652 | 1.203747e-49 | 6.403727e-47 |
| MsG0380014248.01 | MsG0480021189.01 | 0.804597 | 2.001120e-49 | 1.036014e-46 |
| MsG0380014537.01 | MsG0480021189.01 | 0.812641 | 3.827127e-51 | 2.446442e-48 |
| MsG0480018653.01 | MsG0480021189.01 | 0.828561 | 8.396667e-55 | 8.375938e-52 |
| MsG0480018883.01 | MsG0480021189.01 | 0.807461 | 4.998459e-50 | 2.785781e-47 |
| MsG0480019416.01 | MsG0480021189.01 | 0.816970 | 4.201383e-52 | 3.020954e-49 |
| MsG0480020025.01 | MsG0480021189.01 | 0.821723 | 3.469440e-53 | 2.845294e-50 |
| MsG0480021056.01 | MsG0480021189.01 | 0.800403 | 1.464987e-48 | 6.815451e-46 |
| MsG0480021189.01 | MsG0480021195.01 | 0.802181 | 6.338035e-49 | 3.084300e-46 |
| MsG0480021189.01 | MsG0480021465.01 | 0.823413 | 1.403986e-53 | 1.207899e-50 |
| MsG0480021189.01 | MsG0480022068.01 | 0.813104 | 3.030951e-51 | 1.961264e-48 |
| MsG0480021189.01 | MsG0480022985.01 | 0.829745 | 4.334740e-55 | 4.477106e-52 |
| MsG0480021189.01 | MsG0480023660.01 | 0.814962 | 1.179431e-51 | 8.026386e-49 |
| MsG0480021189.01 | MsG0580024197.01 | 0.817729 | 2.835177e-52 | 2.081336e-49 |
| MsG0480021189.01 | MsG0580024790.01 | -0.820037 | 8.474112e-53 | 6.631490e-50 |
| MsG0480021189.01 | MsG0580025135.01 | 0.812957 | 3.264303e-51 | 2.103939e-48 |
| MsG0480021189.01 | MsG0580025467.01 | 0.805417 | 1.348517e-49 | 7.130501e-47 |
| MsG0480021189.01 | MsG0580025772.01 | 0.811863 | 5.658304e-51 | 3.542077e-48 |
| MsG0480021189.01 | MsG0580025987.01 | 0.820685 | 6.019991e-53 | 4.795685e-50 |
| MsG0480021189.01 | MsG0580029236.01 | 0.851185 | 1.046582e-60 | 2.101117e-57 |
| MsG0480021189.01 | MsG0580029376.01 | 0.804685 | 1.918942e-49 | 9.957329e-47 |
| MsG0480021189.01 | MsG0580029925.01 | 0.809547 | 1.792617e-50 | 1.055574e-47 |
| MsG0480021189.01 | MsG0580030000.01 | 0.801601 | 8.335561e-49 | 3.996981e-46 |
| MsG0480021189.01 | MsG0680030413.01 | 0.821277 | 4.398858e-53 | 3.562511e-50 |
| MsG0480021189.01 | MsG0680030882.01 | 0.804604 | 1.995025e-49 | 1.033042e-46 |
| MsG0480021189.01 | MsG0680031264.01 | 0.807171 | 5.758610e-50 | 3.185227e-47 |
| MsG0480021189.01 | MsG0680032827.01 | 0.802142 | 6.453961e-49 | 3.137702e-46 |
| MsG0480021189.01 | MsG0680034230.01 | 0.816729 | 4.758278e-52 | 3.398554e-49 |
| MsG0480021189.01 | MsG0680034234.01 | 0.801969 | 7.004930e-49 | 3.390474e-46 |
| MsG0480021189.01 | MsG0680034739.01 | 0.802000 | 6.903908e-49 | 3.344287e-46 |
| MsG0480021189.01 | MsG0780036593.01 | 0.805426 | 1.342660e-49 | 7.101119e-47 |
| MsG0480021189.01 | MsG0780036602.01 | 0.804361 | 2.241001e-49 | 1.153208e-46 |
| MsG0480021189.01 | MsG0780037054.01 | 0.807295 | 5.420069e-50 | 3.007729e-47 |
| MsG0480021189.01 | MsG0780037141.01 | 0.814690 | 1.355078e-51 | 9.154467e-49 |
| MsG0480021189.01 | MsG0780037965.01 | 0.800488 | 1.407686e-48 | 6.562862e-46 |
| MsG0480021189.01 | MsG0780039139.01 | 0.807653 | 4.550702e-50 | 2.548789e-47 |
| MsG0480021189.01 | MsG0780039170.01 | 0.814131 | 1.801130e-51 | 1.198526e-48 |
| MsG0480021189.01 | MsG0780039443.01 | 0.805827 | 1.106331e-49 | 5.911910e-47 |
| MsG0480021189.01 | MsG0780039867.01 | -0.809193 | 2.135162e-50 | 1.245488e-47 |
| MsG0480021189.01 | MsG0780040888.01 | 0.826255 | 2.999597e-54 | 2.798408e-51 |
| MsG0480021189.01 | MsG0780041187.01 | 0.807956 | 3.922434e-50 | 2.214497e-47 |
| MsG0480021189.01 | MsG0780041391.01 | 0.817785 | 2.754373e-52 | 2.025106e-49 |
| MsG0480021189.01 | MsG0780041416.01 | 0.816201 | 6.247043e-52 | 4.398201e-49 |
| MsG0480021189.01 | MsG0780041440.01 | 0.820910 | 5.344331e-53 | 4.284658e-50 |
| MsG0480021189.01 | MsG0880041861.01 | 0.816214 | 6.204854e-52 | 4.370010e-49 |
| MsG0480021189.01 | MsG0880042700.01 | 0.812413 | 4.292553e-51 | 2.727622e-48 |
| MsG0480021189.01 | MsG0880043157.01 | 0.804147 | 2.483630e-49 | 1.271042e-46 |
| MsG0480021189.01 | MsG0880043509.01 | 0.815146 | 1.073571e-51 | 7.342314e-49 |
| MsG0480021189.01 | MsG0880043937.01 | 0.824447 | 8.033279e-54 | 7.116778e-51 |
| MsG0480021189.01 | MsG0880043941.01 | 0.815416 | 9.348172e-52 | 6.441120e-49 |
| MsG0480021189.01 | MsG0880044394.01 | 0.802134 | 6.481080e-49 | 3.150213e-46 |
| MsG0480021189.01 | MsG0880044728.01 | 0.833278 | 5.846556e-56 | 6.700692e-53 |
| MsG0480021189.01 | MsG0880045111.01 | 0.818298 | 2.108375e-52 | 1.572102e-49 |
| MsG0480021189.01 | MsG0880045362.01 | 0.832145 | 1.117143e-55 | 1.237782e-52 |
| MsG0480021189.01 | MsG0880045736.01 | 0.809350 | 1.976081e-50 | 1.157578e-47 |
| MsG0480021189.01 | MsG0880045765.01 | 0.808687 | 2.740300e-50 | 1.576983e-47 |
| MsG0480021189.01 | MsG0880045992.01 | 0.816967 | 4.208561e-52 | 3.025885e-49 |
| MsG0480021189.01 | MsG0880046724.01 | 0.814836 | 1.257717e-51 | 8.530182e-49 |
| MsG0480021189.01 | MsG0880047052.01 | 0.812886 | 3.382733e-51 | 2.176311e-48 |
| MsG0480021189.01 | MsG0880047764.01 | 0.838005 | 3.717029e-57 | 4.913738e-54 |
| MsG0380015555.01 | MsG0480021189.01 | 0.831177 | 1.935040e-55 | 2.083557e-52 |
| MsG0380016285.01 | MsG0480021189.01 | 0.804228 | 2.389128e-49 | 1.225164e-46 |
| MsG0380016529.01 | MsG0480021189.01 | 0.813664 | 2.282505e-51 | 1.499763e-48 |
| MsG0380016695.01 | MsG0480021189.01 | 0.804080 | 2.564287e-49 | 1.310093e-46 |
| MsG0380017202.01 | MsG0480021189.01 | 0.821736 | 3.445915e-53 | 2.826980e-50 |
| MsG0380017293.01 | MsG0480021189.01 | 0.820341 | 7.220403e-53 | 5.697744e-50 |
| MsG0380017461.01 | MsG0480021189.01 | 0.820057 | 8.386100e-53 | 6.566266e-50 |
| MsG0380017589.01 | MsG0480021189.01 | 0.801936 | 7.115566e-49 | 3.441210e-46 |
| MsG0480018236.01 | MsG0480021189.01 | 0.820609 | 6.266415e-53 | 4.981491e-50 |
| MsG0480018319.01 | MsG0480021189.01 | 0.811117 | 8.218694e-51 | 5.044496e-48 |
| MsG0280006491.01 | MsG0480021189.01 | 0.806606 | 7.581322e-50 | 4.132556e-47 |
| MsG0280006593.01 | MsG0480021189.01 | 0.820940 | 5.258331e-53 | 4.219303e-50 |
| MsG0280007575.01 | MsG0480021189.01 | 0.808264 | 3.373203e-50 | 1.920031e-47 |
| MsG0280007955.01 | MsG0480021189.01 | 0.871197 | 8.208416e-67 | 3.282283e-63 |
| MsG0280008112.01 | MsG0480021189.01 | 0.830274 | 3.220546e-55 | 3.377030e-52 |
| MsG0280008113.01 | MsG0480021189.01 | 0.809410 | 1.918895e-50 | 1.125841e-47 |
| MsG0280008377.01 | MsG0480021189.01 | 0.813867 | 2.059551e-51 | 1.360697e-48 |
| MsG0280008866.01 | MsG0480021189.01 | 0.825481 | 4.578755e-54 | 4.178403e-51 |
| MsG0280010184.01 | MsG0480021189.01 | 0.816390 | 5.668241e-52 | 4.011124e-49 |
| MsG0280010349.01 | MsG0480021189.01 | 0.811741 | 6.017026e-51 | 3.754435e-48 |
| MsG0280010366.01 | MsG0480021189.01 | 0.826679 | 2.376307e-54 | 2.243914e-51 |
| MsG0280010434.01 | MsG0480021189.01 | 0.829926 | 3.915552e-55 | 4.064888e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTGATAATTCCAAATTT+TGG | 0.177386 | 4:+54884914 | None:intergenic |
| TGGAATTATCAAACTTCATT+TGG | 0.241574 | 4:-54884906 | MsG0480021189.01.T01:CDS |
| GGAATTATCAAACTTCATTT+GGG | 0.304283 | 4:-54884905 | MsG0480021189.01.T01:CDS |
| CCTGTGAAGCGTCCAAAATT+TGG | 0.307340 | 4:-54884926 | MsG0480021189.01.T01:CDS |
| TTCTCATTCTCATCGTCCTT+TGG | 0.375232 | 4:-54884987 | MsG0480021189.01.T01:CDS |
| CTCCAAGGTTGCTGCTGTGT+TGG | 0.404120 | 4:-54885026 | MsG0480021189.01.T01:CDS |
| GATGTGGTAATTGATTTCTT+CGG | 0.431738 | 4:+54885103 | None:intergenic |
| CTCCAACACAGCAGCAACCT+TGG | 0.459267 | 4:+54885024 | None:intergenic |
| TCATCCGCCACCTCAATCTC+AGG | 0.485728 | 4:+54885058 | None:intergenic |
| TGTAACTTCTCCTGAGATTG+AGG | 0.493679 | 4:-54885068 | MsG0480021189.01.T01:CDS |
| TTCTCCTGAGATTGAGGTGG+CGG | 0.496078 | 4:-54885062 | MsG0480021189.01.T01:CDS |
| TCAGGAGAAGTTACAACAGC+AGG | 0.535817 | 4:+54885076 | None:intergenic |
| TTCCACCTCTACTGATGTTG+CGG | 0.547904 | 4:-54884957 | MsG0480021189.01.T01:CDS |
| AGGGAGTAATAAACTAAACT+AGG | 0.582151 | 4:+54884845 | None:intergenic |
| AGCCGCAACATCAGTAGAGG+TGG | 0.595759 | 4:+54884955 | None:intergenic |
| GGATGATTTATCTGTCTCCA+AGG | 0.604171 | 4:-54885041 | MsG0480021189.01.T01:CDS |
| AACTTCTCCTGAGATTGAGG+TGG | 0.619014 | 4:-54885065 | MsG0480021189.01.T01:CDS |
| GAATTATCAAACTTCATTTG+GGG | 0.621507 | 4:-54884904 | MsG0480021189.01.T01:CDS |
| CTTAGCCGCAACATCAGTAG+AGG | 0.638758 | 4:+54884952 | None:intergenic |
| TACAACAGCAGGTTGAGATG+TGG | 0.648423 | 4:+54885087 | None:intergenic |
| GAGGTGGAACACTTAACCAA+AGG | 0.667335 | 4:+54884971 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTGATAATTCCAAATTT+TGG | + | Chr4:54884985-54885004 | None:intergenic | 20.0% |
| ! | GAATTATCAAACTTCATTTG+GGG | - | Chr4:54884992-54885011 | MsG0480021189.01.T01:CDS | 25.0% |
| ! | GGAATTATCAAACTTCATTT+GGG | - | Chr4:54884991-54885010 | MsG0480021189.01.T01:CDS | 25.0% |
| ! | TGGAATTATCAAACTTCATT+TGG | - | Chr4:54884990-54885009 | MsG0480021189.01.T01:CDS | 25.0% |
| AGGGAGTAATAAACTAAACT+AGG | + | Chr4:54885054-54885073 | None:intergenic | 30.0% | |
| ! | GATGTGGTAATTGATTTCTT+CGG | + | Chr4:54884796-54884815 | None:intergenic | 30.0% |
| !! | AAACGACACTCATTTTGAAA+CGG | + | Chr4:54885077-54885096 | None:intergenic | 30.0% |
| !!! | TTCAAAATGAGTGTCGTTTT+AGG | - | Chr4:54885078-54885097 | MsG0480021189.01.T01:CDS | 30.0% |
| GGATGATTTATCTGTCTCCA+AGG | - | Chr4:54884855-54884874 | MsG0480021189.01.T01:CDS | 40.0% | |
| TGTAACTTCTCCTGAGATTG+AGG | - | Chr4:54884828-54884847 | MsG0480021189.01.T01:CDS | 40.0% | |
| TTCTCATTCTCATCGTCCTT+TGG | - | Chr4:54884909-54884928 | MsG0480021189.01.T01:CDS | 40.0% | |
| !!! | GACACTCATTTTGAAACGGA+GGG | + | Chr4:54885073-54885092 | None:intergenic | 40.0% |
| AACTTCTCCTGAGATTGAGG+TGG | - | Chr4:54884831-54884850 | MsG0480021189.01.T01:CDS | 45.0% | |
| GAGGTGGAACACTTAACCAA+AGG | + | Chr4:54884928-54884947 | None:intergenic | 45.0% | |
| TCAGGAGAAGTTACAACAGC+AGG | + | Chr4:54884823-54884842 | None:intergenic | 45.0% | |
| TTCCACCTCTACTGATGTTG+CGG | - | Chr4:54884939-54884958 | MsG0480021189.01.T01:CDS | 45.0% | |
| ! | CCAAATTTTGGACGCTTCAC+AGG | + | Chr4:54884973-54884992 | None:intergenic | 45.0% |
| ! | CCTGTGAAGCGTCCAAAATT+TGG | - | Chr4:54884970-54884989 | MsG0480021189.01.T01:CDS | 45.0% |
| ! | TACAACAGCAGGTTGAGATG+TGG | + | Chr4:54884812-54884831 | None:intergenic | 45.0% |
| !!! | CGACACTCATTTTGAAACGG+AGG | + | Chr4:54885074-54885093 | None:intergenic | 45.0% |
| CTTAGCCGCAACATCAGTAG+AGG | + | Chr4:54884947-54884966 | None:intergenic | 50.0% | |
| TTCTCCTGAGATTGAGGTGG+CGG | - | Chr4:54884834-54884853 | MsG0480021189.01.T01:CDS | 50.0% | |
| AGCCGCAACATCAGTAGAGG+TGG | + | Chr4:54884944-54884963 | None:intergenic | 55.0% | |
| CTCCAACACAGCAGCAACCT+TGG | + | Chr4:54884875-54884894 | None:intergenic | 55.0% | |
| CTCCAAGGTTGCTGCTGTGT+TGG | - | Chr4:54884870-54884889 | MsG0480021189.01.T01:CDS | 55.0% | |
| TCATCCGCCACCTCAATCTC+AGG | + | Chr4:54884841-54884860 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 54884784 | 54885134 | 54884784 | ID=MsG0480021189.01;Name=MsG0480021189.01 |
| Chr4 | mRNA | 54884784 | 54885134 | 54884784 | ID=MsG0480021189.01.T01;Parent=MsG0480021189.01;Name=MsG0480021189.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr4 | exon | 54884784 | 54885134 | 54884784 | ID=MsG0480021189.01.T01:exon:6637;Parent=MsG0480021189.01.T01 |
| Chr4 | CDS | 54884784 | 54885134 | 54884784 | ID=MsG0480021189.01.T01:cds;Parent=MsG0480021189.01.T01 |
| Gene Sequence |
| Protein sequence |
