AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017511.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017511.01.T01 MTR_3g108090 58.824 119 17 2 47 133 189 307 5.51e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCTTCTTGGCATTCT+TGG 0.174540 3:+97187426 None:intergenic
AGCAGCGTCCATTTCTTTAC+TGG 0.341268 3:+97187265 None:intergenic
CAGGATAAGAAAGGTGTAGA+TGG 0.373922 3:-97187487 MsG0380017511.01.T01:CDS
GGGTTTGTCCTCACTGGTTG+AGG 0.379223 3:+97189016 None:intergenic
GTTGAGGGTCGGAAAATTGA+TGG 0.390978 3:+97189032 None:intergenic
GGTTTGTCCTCACTGGTTGA+GGG 0.425859 3:+97189017 None:intergenic
TGCTAGAGCTGCTGCTCAAG+AGG 0.430832 3:-97187243 MsG0380017511.01.T01:CDS
GCACAGGTTGTTGGTTGTAA+TGG 0.445016 3:+97187171 None:intergenic
TTCTTTACCGCACAGGTTGT+TGG 0.457072 3:+97187162 None:intergenic
TGTATTTGCAGGTGCACCTC+AGG 0.467621 3:-97187506 MsG0380017511.01.T01:intron
CGTTAGACTCTTCACCTCCT+TGG 0.470055 3:+97187388 None:intergenic
GGTGTAGATGGGGATGTAGA+TGG 0.477288 3:-97187475 MsG0380017511.01.T01:CDS
ATGCCAAGAAGAAGAAAAGA+AGG 0.489841 3:-97187420 MsG0380017511.01.T01:CDS
AGCAAGGTTCTTTACCGCAC+AGG 0.490107 3:+97187155 None:intergenic
GTTCTTCTCTTTCTTCTTAG+CGG 0.490710 3:+97187193 None:intergenic
CACCGTTGAATGCAACCTCA+TGG 0.509050 3:-97189105 MsG0380017511.01.T01:CDS
CTGCTCAAGAGGCTGCTGCA+AGG 0.511826 3:-97187232 MsG0380017511.01.T01:CDS
AAATTGATGGGGCGGTGCGG+TGG 0.523186 3:+97189045 None:intergenic
AGGATAAGAAAGGTGTAGAT+GGG 0.528793 3:-97187486 MsG0380017511.01.T01:CDS
AAAGAAGGATAAAGCTTCCA+AGG 0.529938 3:-97187405 MsG0380017511.01.T01:CDS
TTGAGGGTCGGAAAATTGAT+GGG 0.531380 3:+97189033 None:intergenic
GCCCATGAGGTTGCATTCAA+CGG 0.535338 3:+97189103 None:intergenic
AGGCTGCTGCAAGGAACGCA+AGG 0.544412 3:-97187223 MsG0380017511.01.T01:CDS
TGCAACCTCATGGGCTGATG+TGG 0.545900 3:-97189095 MsG0380017511.01.T01:CDS
ACCGTTGAATGCAACCTCAT+GGG 0.552821 3:-97189104 MsG0380017511.01.T01:CDS
TCTACACCTTTCTTATCCTG+AGG 0.571217 3:+97187490 None:intergenic
TGTCCTCACTGGTTGAGGGT+CGG 0.571428 3:+97189021 None:intergenic
GGAAAATTGATGGGGCGGTG+CGG 0.586176 3:+97189042 None:intergenic
GGTGCACCTCAGGATAAGAA+AGG 0.599338 3:-97187496 MsG0380017511.01.T01:CDS
GGATAAGAAAGGTGTAGATG+GGG 0.604616 3:-97187485 MsG0380017511.01.T01:CDS
GGGTCGGAAAATTGATGGGG+CGG 0.656574 3:+97189037 None:intergenic
GTCATCCACATCAGCCCATG+AGG 0.661130 3:+97189090 None:intergenic
TGAGGGTCGGAAAATTGATG+GGG 0.695646 3:+97189034 None:intergenic
TGAGGTTGCATTCAACGGTG+CGG 0.696515 3:+97189108 None:intergenic
ATTACAACCAACAACCTGTG+CGG 0.714310 3:-97187169 MsG0380017511.01.T01:CDS
GAAGGATAAAGCTTCCAAGG+AGG 0.726948 3:-97187402 MsG0380017511.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAAAAAATTATAAATGC+TGG + Chr3:97188676-97188695 None:intergenic 15.0%
!! AAAATTGCAAAGTAAAAATC+TGG + Chr3:97188161-97188180 None:intergenic 20.0%
!! AGTTTATCATTACTATTCAA+AGG + Chr3:97188027-97188046 None:intergenic 20.0%
!! TGAATAGTAATGATAAACTT+TGG - Chr3:97188028-97188047 MsG0380017511.01.T01:intron 20.0%
!! TTACATTATTTGTAGGATTA+TGG - Chr3:97188478-97188497 MsG0380017511.01.T01:intron 20.0%
!!! AACATTTTGAAGATTATCTT+AGG + Chr3:97187815-97187834 None:intergenic 20.0%
!!! AATAAATAGGTTTTGTTACA+TGG - Chr3:97187953-97187972 MsG0380017511.01.T01:intron 20.0%
!!! ATAAATAGGTTTTGTTACAT+GGG - Chr3:97187954-97187973 MsG0380017511.01.T01:intron 20.0%
!!! TAAGTATTTGAAAAGCTTTT+CGG + Chr3:97187778-97187797 None:intergenic 20.0%
!!! TTTACTTTGCAATTTTTAAC+AGG - Chr3:97188165-97188184 MsG0380017511.01.T01:intron 20.0%
! ACAATAACAAAAAAACATCC+AGG + Chr3:97187358-97187377 None:intergenic 25.0%
! ACACAATTATCAAAAAGATC+AGG + Chr3:97187901-97187920 None:intergenic 25.0%
! GAAAAAATCCAGTAAAGAAA+TGG - Chr3:97189008-97189027 MsG0380017511.01.T01:intron 25.0%
! TATCTTAGGATAAAACATGT+TGG + Chr3:97187801-97187820 None:intergenic 25.0%
! TGTCTATGCGATAAATAAAT+AGG - Chr3:97187940-97187959 MsG0380017511.01.T01:intron 25.0%
!! ACAAGTTGAAAATAAGGTAT+AGG - Chr3:97187735-97187754 MsG0380017511.01.T01:intron 25.0%
!! ATGAACACAAGTTGAAAATA+AGG - Chr3:97187729-97187748 MsG0380017511.01.T01:intron 25.0%
!! TTGATAATTGTGTTTCTTTG+AGG - Chr3:97187908-97187927 MsG0380017511.01.T01:intron 25.0%
!! TTGATGACTATTGAGATTAA+GGG - Chr3:97187506-97187525 MsG0380017511.01.T01:intron 25.0%
!!! ATGTCATTTTTCTGTTTGTT+TGG - Chr3:97187412-97187431 MsG0380017511.01.T01:CDS 25.0%
!!! GAAGAAGATATTTTAGATGA+AGG - Chr3:97188199-97188218 MsG0380017511.01.T01:intron 25.0%
!!! TAAATAGGTTTTGTTACATG+GGG - Chr3:97187955-97187974 MsG0380017511.01.T01:intron 25.0%
!!! TATGGTTTATTTTTGAGTTC+AGG - Chr3:97188496-97188515 MsG0380017511.01.T01:intron 25.0%
ATTCGCAAAATTGTATTTGC+AGG - Chr3:97188764-97188783 MsG0380017511.01.T01:intron 30.0%
CTGCATCTTACATTATTTGT+AGG - Chr3:97188471-97188490 MsG0380017511.01.T01:intron 30.0%
TCAGCGAAAAAAGATGAAAA+TGG + Chr3:97188615-97188634 None:intergenic 30.0%
TTGTGTGTTTATTGCTTGAA+TGG - Chr3:97188114-97188133 MsG0380017511.01.T01:intron 30.0%
TTTATGTGCTTACTTTGCTT+TGG - Chr3:97187852-97187871 MsG0380017511.01.T01:intron 30.0%
! TATCCTTCTTTTCTTCTTCT+TGG + Chr3:97188867-97188886 None:intergenic 30.0%
!! ATTTTCAACATCAGAAGTGT+TGG + Chr3:97188925-97188944 None:intergenic 30.0%
!! GTTGATGACTATTGAGATTA+AGG - Chr3:97187505-97187524 MsG0380017511.01.T01:intron 30.0%
!! TGTTGTTGTTTATTGATTCC+TGG - Chr3:97187337-97187356 MsG0380017511.01.T01:intron 30.0%
!!! TTTTCAACATCAGAAGTGTT+GGG + Chr3:97188924-97188943 None:intergenic 30.0%
AAAGAAGGATAAAGCTTCCA+AGG - Chr3:97188876-97188895 MsG0380017511.01.T01:intron 35.0%
AATAATGTAAGATGCAGCTC+TGG + Chr3:97188468-97188487 None:intergenic 35.0%
AATGAAAGCAATGATGGTCA+AGG - Chr3:97188415-97188434 MsG0380017511.01.T01:intron 35.0%
ACACAGAATGAAAGCAATGA+TGG - Chr3:97188409-97188428 MsG0380017511.01.T01:intron 35.0%
ATGCCAAGAAGAAGAAAAGA+AGG - Chr3:97188861-97188880 MsG0380017511.01.T01:intron 35.0%
GTTCTTCTCTTTCTTCTTAG+CGG + Chr3:97189091-97189110 None:intergenic 35.0%
TAAATACCTGGGATTCATCT+TGG + Chr3:97188441-97188460 None:intergenic 35.0%
TCACTTTATGTGCTTAGCTA+AGG - Chr3:97187386-97187405 MsG0380017511.01.T01:intron 35.0%
TCAGGTATCCTAATAAGAAC+AGG + Chr3:97187883-97187902 None:intergenic 35.0%
TGAACAAACCTCAAAATGCT+CGG + Chr3:97187289-97187308 None:intergenic 35.0%
TGGTTATGCCTGTTCTTATT+AGG - Chr3:97187872-97187891 MsG0380017511.01.T01:intron 35.0%
TTCTGATGTTGAAAATGCAG+AGG - Chr3:97188929-97188948 MsG0380017511.01.T01:intron 35.0%
TTTCTTCTTCTTGGCATTCT+TGG + Chr3:97188858-97188877 None:intergenic 35.0%
! AGGATAAGAAAGGTGTAGAT+GGG - Chr3:97188795-97188814 MsG0380017511.01.T01:intron 35.0%
! GCTCTGGATAATAAATACCT+GGG + Chr3:97188452-97188471 None:intergenic 35.0%
!! AGCTCTGGATAATAAATACC+TGG + Chr3:97188453-97188472 None:intergenic 35.0%
!! TATTTTAGATGAAGGTGACG+AGG - Chr3:97188207-97188226 MsG0380017511.01.T01:intron 35.0%
!!! CTGGTCTTTTTTTCTCTGTT+TGG - Chr3:97188558-97188577 MsG0380017511.01.T01:intron 35.0%
!!! TTTCATCTTTTTTCGCTGAC+AGG - Chr3:97188616-97188635 MsG0380017511.01.T01:intron 35.0%
AAGCACATAAAGTGAGCAAC+AGG + Chr3:97187381-97187400 None:intergenic 40.0%
ATGCATTCGTTCTACACTAC+TGG - Chr3:97188539-97188558 MsG0380017511.01.T01:intron 40.0%
ATTACAACCAACAACCTGTG+CGG - Chr3:97189112-97189131 MsG0380017511.01.T01:CDS 40.0%
CCAAAAAGGAAGGAAGAAGA+AGG - Chr3:97188346-97188365 MsG0380017511.01.T01:intron 40.0%
CTAGATCTTTGAGCAAAAGC+AGG + Chr3:97188590-97188609 None:intergenic 40.0%
GAACAAACCTCAAAATGCTC+GGG + Chr3:97187288-97187307 None:intergenic 40.0%
TAGAACGAATGCATGAGCAA+CGG + Chr3:97188533-97188552 None:intergenic 40.0%
TCTACACCTTTCTTATCCTG+AGG + Chr3:97188794-97188813 None:intergenic 40.0%
TGATGTTGAAAATGCAGAGG+AGG - Chr3:97188932-97188951 MsG0380017511.01.T01:intron 40.0%
TTGAGGGTCGGAAAATTGAT+GGG + Chr3:97187251-97187270 None:intergenic 40.0%
! CAGGATAAGAAAGGTGTAGA+TGG - Chr3:97188794-97188813 MsG0380017511.01.T01:intron 40.0%
! CCTTCTTCTTCCTTCCTTTT+TGG + Chr3:97188349-97188368 None:intergenic 40.0%
! GGATAAGAAAGGTGTAGATG+GGG - Chr3:97188796-97188815 MsG0380017511.01.T01:intron 40.0%
!! CTTTTTGGAGAGTTGACGTT+CGG + Chr3:97188334-97188353 None:intergenic 40.0%
!! TGAGGCTCTTTAGCTGATTT+TGG - Chr3:97188382-97188401 MsG0380017511.01.T01:intron 40.0%
ACCGTTGAATGCAACCTCAT+GGG - Chr3:97187177-97187196 MsG0380017511.01.T01:CDS 45.0%
AGCAGCGTCCATTTCTTTAC+TGG + Chr3:97189019-97189038 None:intergenic 45.0%
CAAGGAGGTGAAGAGTCTAA+CGG - Chr3:97188894-97188913 MsG0380017511.01.T01:intron 45.0%
CGAACGTCAACTCTCCAAAA+AGG - Chr3:97188332-97188351 MsG0380017511.01.T01:intron 45.0%
CGTCAACTCTCCAAAAAGGA+AGG - Chr3:97188336-97188355 MsG0380017511.01.T01:intron 45.0%
GAAGGAACTTGAAGAGCTTG+AGG - Chr3:97188364-97188383 MsG0380017511.01.T01:intron 45.0%
GAAGGATAAAGCTTCCAAGG+AGG - Chr3:97188879-97188898 MsG0380017511.01.T01:intron 45.0%
GTGCATGTCAATCTAGATGC+AGG - Chr3:97188710-97188729 MsG0380017511.01.T01:intron 45.0%
GTTGAGGGTCGGAAAATTGA+TGG + Chr3:97187252-97187271 None:intergenic 45.0%
TCAGCATTCTTCTTGACCTC+AGG + Chr3:97188288-97188307 None:intergenic 45.0%
TGAGGGTCGGAAAATTGATG+GGG + Chr3:97187250-97187269 None:intergenic 45.0%
TTCTTCTTGACCTCAGGTTC+AGG + Chr3:97188282-97188301 None:intergenic 45.0%
TTTAGATGAAGGTGACGAGG+AGG - Chr3:97188210-97188229 MsG0380017511.01.T01:intron 45.0%
! GCACAGGTTGTTGGTTGTAA+TGG + Chr3:97189113-97189132 None:intergenic 45.0%
CACCGTTGAATGCAACCTCA+TGG - Chr3:97187176-97187195 MsG0380017511.01.T01:CDS 50.0%
CACTGTGAAACCTGAACCTG+AGG - Chr3:97188269-97188288 MsG0380017511.01.T01:intron 50.0%
CGTTAGACTCTTCACCTCCT+TGG + Chr3:97188896-97188915 None:intergenic 50.0%
GCCCATGAGGTTGCATTCAA+CGG + Chr3:97187181-97187200 None:intergenic 50.0%
GGTGCACCTCAGGATAAGAA+AGG - Chr3:97188785-97188804 MsG0380017511.01.T01:intron 50.0%
GGTGTAGATGGGGATGTAGA+TGG - Chr3:97188806-97188825 MsG0380017511.01.T01:intron 50.0%
TCAAGGCCAAGATGAATCCC+AGG - Chr3:97188432-97188451 MsG0380017511.01.T01:intron 50.0%
TCAGGTTTCACAGTGTGCTC+TGG + Chr3:97188264-97188283 None:intergenic 50.0%
TGAAGTTCCAGCTGCACCAA+AGG - Chr3:97188305-97188324 MsG0380017511.01.T01:intron 50.0%
TGAGGTTGCATTCAACGGTG+CGG + Chr3:97187176-97187195 None:intergenic 50.0%
TGTATTTGCAGGTGCACCTC+AGG - Chr3:97188775-97188794 MsG0380017511.01.T01:intron 50.0%
! GGACAAACCCGAGCATTTTG+AGG - Chr3:97187278-97187297 MsG0380017511.01.T01:CDS 50.0%
! GGTTTGTCCTCACTGGTTGA+GGG + Chr3:97187267-97187286 None:intergenic 50.0%
! GTTACATGGGGTGCGCATTT+TGG - Chr3:97187967-97187986 MsG0380017511.01.T01:intron 50.0%
AGTTCCAGCTGCACCAAAGG+AGG - Chr3:97188308-97188327 MsG0380017511.01.T01:intron 55.0%
AGTTGACGTTCGGCCTCCTT+TGG + Chr3:97188324-97188343 None:intergenic 55.0%
ATGCTCGGGTTTGTCCTCAC+TGG + Chr3:97187274-97187293 None:intergenic 55.0%
GGAAAATTGATGGGGCGGTG+CGG + Chr3:97187242-97187261 None:intergenic 55.0%
GGGTCGGAAAATTGATGGGG+CGG + Chr3:97187247-97187266 None:intergenic 55.0%
GTCATCCACATCAGCCCATG+AGG + Chr3:97187194-97187213 None:intergenic 55.0%
TGAAGGTGACGAGGAGGTAG+AGG - Chr3:97188216-97188235 MsG0380017511.01.T01:intron 55.0%
TGCTAGAGCTGCTGCTCAAG+AGG - Chr3:97189038-97189057 MsG0380017511.01.T01:CDS 55.0%
TTTCCGACCCTCAACCAGTG+AGG - Chr3:97187257-97187276 MsG0380017511.01.T01:CDS 55.0%
! GGGTTTGTCCTCACTGGTTG+AGG + Chr3:97187268-97187287 None:intergenic 55.0%
! TGCAACCTCATGGGCTGATG+TGG - Chr3:97187186-97187205 MsG0380017511.01.T01:CDS 55.0%
! TGTCCTCACTGGTTGAGGGT+CGG + Chr3:97187263-97187282 None:intergenic 55.0%
AAATTGATGGGGCGGTGCGG+TGG + Chr3:97187239-97187258 None:intergenic 60.0%
AGGCTGCTGCAAGGAACGCA+AGG - Chr3:97189058-97189077 MsG0380017511.01.T01:CDS 60.0%
CTGCTCAAGAGGCTGCTGCA+AGG - Chr3:97189049-97189068 MsG0380017511.01.T01:CDS 60.0%
! TCGGCCTCCTTTGGTGCAGC+TGG + Chr3:97188315-97188334 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 97187166 97189137 97187166 ID=MsG0380017511.01;Name=MsG0380017511.01
Chr3 mRNA 97187166 97189137 97187166 ID=MsG0380017511.01.T01;Parent=MsG0380017511.01;Name=MsG0380017511.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|4|0|133
Chr3 exon 97189030 97189137 97189030 ID=MsG0380017511.01.T01:exon:14118;Parent=MsG0380017511.01.T01
Chr3 exon 97188083 97188116 97188083 ID=MsG0380017511.01.T01:exon:14119;Parent=MsG0380017511.01.T01
Chr3 exon 97187403 97187517 97187403 ID=MsG0380017511.01.T01:exon:14120;Parent=MsG0380017511.01.T01
Chr3 exon 97187166 97187310 97187166 ID=MsG0380017511.01.T01:exon:14121;Parent=MsG0380017511.01.T01
Chr3 CDS 97189030 97189137 97189030 ID=MsG0380017511.01.T01:cds;Parent=MsG0380017511.01.T01
Chr3 CDS 97188083 97188116 97188083 ID=MsG0380017511.01.T01:cds;Parent=MsG0380017511.01.T01
Chr3 CDS 97187403 97187517 97187403 ID=MsG0380017511.01.T01:cds;Parent=MsG0380017511.01.T01
Chr3 CDS 97187166 97187310 97187166 ID=MsG0380017511.01.T01:cds;Parent=MsG0380017511.01.T01
Gene Sequence

>MsG0380017511.01.T01

TTGGGCTCCGCACCGTTGAATGCAACCTCATGGGCTGATGTGGATGACGACGATGATGATTATTATCTGACCACCGCACCGCCCCATCAATTTTCCGACCCTCAACCAGAATCTGAGAGTGAAGAAGATATTTTAGATGAAGGTGCACCTCAGGATAAGAAAGGTGTAGATGGGGATGTAGATGGCGAAAAGAAAGAAATCACTGCTGAGTCCAAGAATGCCAAGAAGAAGAAAAGAAGGATAAAGCTTCCAAGGAGTTGCTTCTATGAGAAGAAAAAATCCAGTAAAGAAATGGACGCTGCTGCTAGAGCTGCTGCTCAAGAGGCTGCTGCAAGGAACGCAAGGCTTGCTGCCGCTAAGAAGAAAGAGAAGAACCATTACAACCAACAACCTGTGCGGTAA

Protein sequence

>MsG0380017511.01.T01

LGSAPLNATSWADVDDDDDDYYLTTAPPHQFSDPQPESESEEDILDEGAPQDKKGVDGDVDGEKKEITAESKNAKKKKRRIKLPRSCFYEKKKSSKEMDAAARAAAQEAAARNARLAAAKKKEKNHYNQQPVR*