AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023396.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023396.01.T01 MTR_8g092180 71.277 94 21 1 4 97 58 145 1.17e-38 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023396.01.T01 AT3G52860 41.053 95 49 2 4 97 68 156 2.49e-15 67.4

Find 18 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATAAAGTTGAAGTTTCT+TGG 0.235328 4:+84667301 None:intergenic
AAAACAAGAACATTTGATTC+AGG 0.280242 4:-84667086 MsG0480023396.01.T01:CDS
AACACAATATTGAGTTGAAC+AGG 0.311642 4:-84667030 MsG0480023396.01.T01:CDS
CAAGCAGGAGATTGCTCTTA+TGG 0.318355 4:-84667143 MsG0480023396.01.T01:intron
ATTTGAAGTTACTCATTTGA+TGG 0.388894 4:+84668903 None:intergenic
TGATGGGAAGGGTGGGGAAA+CGG 0.441112 4:+84668920 None:intergenic
AAAAGAGCTGTCAGATCAGT+TGG 0.445481 4:-84667056 MsG0480023396.01.T01:CDS
CTCATTTGATGGGAAGGGTG+GGG 0.499240 4:+84668914 None:intergenic
AAAAGCTGAAATGCTTAGAA+AGG 0.503198 4:-84667250 MsG0480023396.01.T01:intron
TACTCATTTGATGGGAAGGG+TGG 0.509595 4:+84668912 None:intergenic
AAGTTACTCATTTGATGGGA+AGG 0.511231 4:+84668908 None:intergenic
TTTGAAGTTACTCATTTGAT+GGG 0.525355 4:+84668904 None:intergenic
ACTCATTTGATGGGAAGGGT+GGG 0.531539 4:+84668913 None:intergenic
AGTTACTCATTTGATGGGAA+GGG 0.563207 4:+84668909 None:intergenic
ATTTATTAGTCTGCAACGTG+AGG 0.597216 4:-84667283 MsG0480023396.01.T01:CDS
GCTCCATAAAATCTCTAGCA+TGG 0.606048 4:+84667326 None:intergenic
AAGAACATTTGATTCAGGAG+TGG 0.607764 4:-84667081 MsG0480023396.01.T01:CDS
ACACAATATTGAGTTGAACA+GGG 0.651621 4:-84667029 MsG0480023396.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATTTTTATATTAAT+TGG - Chr4:84667155-84667174 MsG0480023396.01.T01:intron 0.0%
!!! AATAATTATTGATTAACATA+TGG + Chr4:84668112-84668131 None:intergenic 10.0%
!!! TAAACAGATTTTAATATATT+TGG - Chr4:84668555-84668574 MsG0480023396.01.T01:intron 10.0%
!! AAAAAGAATATATATGTACA+AGG + Chr4:84667688-84667707 None:intergenic 15.0%
!! TTCTAAACTTTAACATAAAA+AGG + Chr4:84667926-84667945 None:intergenic 15.0%
!!! AAGTCAAATGATAATTATTT+TGG - Chr4:84667327-84667346 MsG0480023396.01.T01:CDS 15.0%
!!! ATTTTGGTTATTTAAGTTTA+AGG - Chr4:84667343-84667362 MsG0480023396.01.T01:CDS 15.0%
!! ATTTGGAAAATATCTTAAGT+TGG - Chr4:84668572-84668591 MsG0480023396.01.T01:intron 20.0%
!! TAAATAAAGTTGAAGTTTCT+TGG + Chr4:84668649-84668668 None:intergenic 20.0%
!! TTATTAGCAAACATAGAAAA+TGG - Chr4:84668515-84668534 MsG0480023396.01.T01:intron 20.0%
!! TTTGGAAAATATCTTAAGTT+GGG - Chr4:84668573-84668592 MsG0480023396.01.T01:intron 20.0%
!!! AATCTTTCAAATCTTGATTT+TGG - Chr4:84667064-84667083 MsG0480023396.01.T01:CDS 20.0%
!!! TTTTTTATTTCACACGTTTT+AGG - Chr4:84668235-84668254 MsG0480023396.01.T01:intron 20.0%
! AAAAAATGCTGTTCCTTAAT+GGG - Chr4:84667708-84667727 MsG0480023396.01.T01:intron 25.0%
! AAAACAAGAACATTTGATTC+AGG - Chr4:84668861-84668880 MsG0480023396.01.T01:intron 25.0%
! AGTTAGATAAGAACATTACA+AGG + Chr4:84668765-84668784 None:intergenic 25.0%
! TAAAAAATGCTGTTCCTTAA+TGG - Chr4:84667707-84667726 MsG0480023396.01.T01:intron 25.0%
! TGATAGATACTAGAAGTTAT+GGG - Chr4:84667570-84667589 MsG0480023396.01.T01:intron 25.0%
! TGTATCTAATTAATGACACA+CGG + Chr4:84667422-84667441 None:intergenic 25.0%
!! ATTTGAAGTTACTCATTTGA+TGG + Chr4:84667047-84667066 None:intergenic 25.0%
!! TTTGAAGTTACTCATTTGAT+GGG + Chr4:84667046-84667065 None:intergenic 25.0%
!!! AAATTGCGTTTTGATTGAAA+TGG - Chr4:84667499-84667518 MsG0480023396.01.T01:intron 25.0%
AAAAGCTGAAATGCTTAGAA+AGG - Chr4:84668697-84668716 MsG0480023396.01.T01:intron 30.0%
AAAGCACAAGTGTATGATAT+GGG - Chr4:84667747-84667766 MsG0480023396.01.T01:intron 30.0%
AATTAGCTGTGAAGTCAAAT+AGG - Chr4:84668388-84668407 MsG0480023396.01.T01:intron 30.0%
ATAAATGCAAGTACAACCTA+TGG + Chr4:84668269-84668288 None:intergenic 30.0%
ATGCTAACATAACATATGTG+AGG - Chr4:84667379-84667398 MsG0480023396.01.T01:intron 30.0%
CAATCAAAACGCAATTTACA+TGG + Chr4:84667496-84667515 None:intergenic 30.0%
CAATCAACTCAATTATCACA+TGG + Chr4:84667879-84667898 None:intergenic 30.0%
CCACACAATTATTACTGAAA+TGG + Chr4:84667527-84667546 None:intergenic 30.0%
CCATTTCAGTAATAATTGTG+TGG - Chr4:84667524-84667543 MsG0480023396.01.T01:intron 30.0%
CTGATAGATACTAGAAGTTA+TGG - Chr4:84667569-84667588 MsG0480023396.01.T01:intron 30.0%
! AAGTGCTGCAAAACTTTATA+AGG + Chr4:84668439-84668458 None:intergenic 30.0%
!! AACACAATATTGAGTTGAAC+AGG - Chr4:84668917-84668936 MsG0480023396.01.T01:CDS 30.0%
!! ACACAATATTGAGTTGAACA+GGG - Chr4:84668918-84668937 MsG0480023396.01.T01:CDS 30.0%
!! CATGTGATAATTGAGTTGAT+TGG - Chr4:84667877-84667896 MsG0480023396.01.T01:intron 30.0%
AACCACAGAAATGAGTGAAA+TGG - Chr4:84668147-84668166 MsG0480023396.01.T01:intron 35.0%
AAGAACATTTGATTCAGGAG+TGG - Chr4:84668866-84668885 MsG0480023396.01.T01:intron 35.0%
AAGTTACTCATTTGATGGGA+AGG + Chr4:84667042-84667061 None:intergenic 35.0%
ACCACAGAAATGAGTGAAAT+GGG - Chr4:84668148-84668167 MsG0480023396.01.T01:intron 35.0%
ACTACAAGCTGTGACTAAAT+AGG - Chr4:84667290-84667309 MsG0480023396.01.T01:CDS 35.0%
AGACATAACTAGAGCAGAAT+AGG + Chr4:84668491-84668510 None:intergenic 35.0%
AGTTACTCATTTGATGGGAA+GGG + Chr4:84667041-84667060 None:intergenic 35.0%
ATTTAGTCACAGCTTGTAGT+AGG + Chr4:84667290-84667309 None:intergenic 35.0%
GACATAACTAGAGCAGAATA+GGG + Chr4:84668490-84668509 None:intergenic 35.0%
TCAACAACTGAATTCCACAA+GGG + Chr4:84667971-84667990 None:intergenic 35.0%
! ATTTAGACCTTTCTAGCCTT+AGG - Chr4:84668287-84668306 MsG0480023396.01.T01:intron 35.0%
! ATTTATTAGTCTGCAACGTG+AGG - Chr4:84668664-84668683 MsG0480023396.01.T01:intron 35.0%
! CTAAGCATTTCAGCTTTTGT+TGG + Chr4:84668695-84668714 None:intergenic 35.0%
! GAAAGCACAAGTGTATGATA+TGG - Chr4:84667746-84667765 MsG0480023396.01.T01:intron 35.0%
! GAGACATTTTGTGTGATCAA+TGG - Chr4:84667839-84667858 MsG0480023396.01.T01:intron 35.0%
!! ATTGAGTTGAACAGGGTTTA+GGG - Chr4:84668925-84668944 MsG0480023396.01.T01:CDS 35.0%
!! TATTGAGTTGAACAGGGTTT+AGG - Chr4:84668924-84668943 MsG0480023396.01.T01:CDS 35.0%
!! TTTAGACCTTTCTAGCCTTA+GGG - Chr4:84668288-84668307 MsG0480023396.01.T01:intron 35.0%
AAAAGAGCTGTCAGATCAGT+TGG - Chr4:84668891-84668910 MsG0480023396.01.T01:intron 40.0%
AACAACTGAATTCCACAAGG+GGG + Chr4:84667969-84667988 None:intergenic 40.0%
AAGTTCCATACAGCCCATTA+AGG + Chr4:84667724-84667743 None:intergenic 40.0%
ATTTGACCCTAAGGCTAGAA+AGG + Chr4:84668297-84668316 None:intergenic 40.0%
CAAAATTGCAGTCAAACCAC+GGG + Chr4:84668324-84668343 None:intergenic 40.0%
CAACAACTGAATTCCACAAG+GGG + Chr4:84667970-84667989 None:intergenic 40.0%
CCAAAATTGCAGTCAAACCA+CGG + Chr4:84668325-84668344 None:intergenic 40.0%
GAGAAAAGAAGGGTGGTAAA+AGG - Chr4:84668036-84668055 MsG0480023396.01.T01:intron 40.0%
GCTCCATAAAATCTCTAGCA+TGG + Chr4:84668624-84668643 None:intergenic 40.0%
GTCAACAACTGAATTCCACA+AGG + Chr4:84667972-84667991 None:intergenic 40.0%
TCCCATTTCACTCATTTCTG+TGG + Chr4:84668152-84668171 None:intergenic 40.0%
! GGTTTTTCTATCAGTCGATG+TGG - Chr4:84668594-84668613 MsG0480023396.01.T01:intron 40.0%
! TAAAGTTTTGCAGCACTTGC+AGG - Chr4:84668441-84668460 MsG0480023396.01.T01:intron 40.0%
! TCGCCATGCTAGAGATTTTA+TGG - Chr4:84668618-84668637 MsG0480023396.01.T01:intron 40.0%
!! TTGAGTTGAACAGGGTTTAG+GGG - Chr4:84668926-84668945 MsG0480023396.01.T01:CDS 40.0%
ACAACTGAATTCCACAAGGG+GGG + Chr4:84667968-84667987 None:intergenic 45.0%
ACGGGTTCTATTTGACCCTA+AGG + Chr4:84668306-84668325 None:intergenic 45.0%
ACTCATTTGATGGGAAGGGT+GGG + Chr4:84667037-84667056 None:intergenic 45.0%
AGCAGAATAGGGACATAAGC+GGG + Chr4:84668479-84668498 None:intergenic 45.0%
ATGAGTGCACCAATCATCCA+TGG - Chr4:84668012-84668031 MsG0480023396.01.T01:intron 45.0%
CAAGCAGGAGATTGCTCTTA+TGG - Chr4:84668804-84668823 MsG0480023396.01.T01:intron 45.0%
CATAAGAGCAATCTCCTGCT+TGG + Chr4:84668806-84668825 None:intergenic 45.0%
CATCCATGGGGAGAAAAGAA+GGG - Chr4:84668026-84668045 MsG0480023396.01.T01:intron 45.0%
CTGTTATGTGTTGTCCAAGC+AGG - Chr4:84668789-84668808 MsG0480023396.01.T01:intron 45.0%
GAGCAGAATAGGGACATAAG+CGG + Chr4:84668480-84668499 None:intergenic 45.0%
TACTCATTTGATGGGAAGGG+TGG + Chr4:84667038-84667057 None:intergenic 45.0%
TATGATTCCTCACACGCTGA+GGG + Chr4:84667781-84667800 None:intergenic 45.0%
TCATCCATGGGGAGAAAAGA+AGG - Chr4:84668025-84668044 MsG0480023396.01.T01:intron 45.0%
TGAGTGCACCAATCATCCAT+GGG - Chr4:84668013-84668032 MsG0480023396.01.T01:intron 45.0%
TTAGGGTCAAATAGAACCCG+TGG - Chr4:84668305-84668324 MsG0480023396.01.T01:intron 45.0%
TTATGATTCCTCACACGCTG+AGG + Chr4:84667782-84667801 None:intergenic 45.0%
! CCGTGGTTTGACTGCAATTT+TGG - Chr4:84668322-84668341 MsG0480023396.01.T01:intron 45.0%
! CTTTTCTCCCCATGGATGAT+TGG + Chr4:84668024-84668043 None:intergenic 45.0%
!! CTGTTCCTTAATGGGCTGTA+TGG - Chr4:84667716-84667735 MsG0480023396.01.T01:intron 45.0%
!! TTCACACGTTTTAGGTCCGA+GGG - Chr4:84668243-84668262 MsG0480023396.01.T01:intron 45.0%
!! TTTCACACGTTTTAGGTCCG+AGG - Chr4:84668242-84668261 MsG0480023396.01.T01:intron 45.0%
CAAGTACAACCTATGGCCCT+CGG + Chr4:84668262-84668281 None:intergenic 50.0%
CTCATTTGATGGGAAGGGTG+GGG + Chr4:84667036-84667055 None:intergenic 50.0%
GAGTGCACCAATCATCCATG+GGG - Chr4:84668014-84668033 MsG0480023396.01.T01:intron 50.0%
ACAACATTGTGCCCCCCTTG+TGG - Chr4:84667954-84667973 MsG0480023396.01.T01:intron 55.0%
CCATGGGGAGAAAAGAAGGG+TGG - Chr4:84668029-84668048 MsG0480023396.01.T01:intron 55.0%
TGATGGGAAGGGTGGGGAAA+CGG + Chr4:84667030-84667049 None:intergenic 55.0%
! CCACCCTTCTTTTCTCCCCA+TGG + Chr4:84668032-84668051 None:intergenic 55.0%
! GGGGGCACAATGTTGTTGCA+AGG + Chr4:84667950-84667969 None:intergenic 55.0%
!! GTTTTAGGTCCGAGGGCCAT+AGG - Chr4:84668250-84668269 MsG0480023396.01.T01:intron 55.0%
GACGATACCCTCAGCGTGTG+AGG - Chr4:84667771-84667790 MsG0480023396.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 84667024 84668945 84667024 ID=MsG0480023396.01;Name=MsG0480023396.01
Chr4 mRNA 84667024 84668945 84667024 ID=MsG0480023396.01.T01;Parent=MsG0480023396.01;Name=MsG0480023396.01.T01;_AED=0.50;_eAED=0.65;_QI=0|0|0|1|0.5|0.33|3|0|97
Chr4 exon 84668915 84668945 84668915 ID=MsG0480023396.01.T01:exon:6163;Parent=MsG0480023396.01.T01
Chr4 exon 84667251 84667378 84667251 ID=MsG0480023396.01.T01:exon:6162;Parent=MsG0480023396.01.T01
Chr4 exon 84667024 84667158 84667024 ID=MsG0480023396.01.T01:exon:6161;Parent=MsG0480023396.01.T01
Chr4 CDS 84668915 84668945 84668915 ID=MsG0480023396.01.T01:cds;Parent=MsG0480023396.01.T01
Chr4 CDS 84667251 84667378 84667251 ID=MsG0480023396.01.T01:cds;Parent=MsG0480023396.01.T01
Chr4 CDS 84667024 84667158 84667024 ID=MsG0480023396.01.T01:cds;Parent=MsG0480023396.01.T01
Gene Sequence

>MsG0480023396.01.T01

GGGCCGTTTCCCCACCCTTCCCATCAAATGATTGGGTTTTTCTATCAGTCGATGTGGATCGCCATGCTAGAGATTTTATGGAGCTGCCAAGAAACTTCAACTTTATTTATTAGTCTGCAACGTGAGGATAAGCCAACAAAAGCTGAAATGCTTAGAAAGGAGATTGCTCTTATGGAAGATGAGCTTAACGAAAAAAACGAGCTGATTAAAAAACAAGAACATTTGATTCAGGAGTGGAAAAAAGAGCTGTCAGATCAGTTGGAAAAACACAATATTGAGTTGAACAGGGTTTAG

Protein sequence

>MsG0480023396.01.T01

GPFPHPSHQMIGFFYQSMWIAMLEILWSCQETSTLFISLQREDKPTKAEMLRKEIALMEDELNEKNELIKKQEHLIQEWKKELSDQLEKHNIELNRV*