AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019810.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019810.01.T01 MTR_7g088680 93.805 113 7 0 11 123 91 203 1.68e-72 215
MsG0480019810.01.T01 MTR_3g092970 65.254 118 35 2 12 123 110 227 1.49e-42 139
MsG0480019810.01.T01 MTR_3g092980 54.400 125 36 2 12 123 102 218 6.85e-36 122
MsG0480019810.01.T01 MTR_3g092980 54.400 125 36 2 12 123 104 220 7.70e-36 122
MsG0480019810.01.T01 MTR_3g093750 52.713 129 41 5 5 123 409 527 2.15e-26 102
MsG0480019810.01.T01 MTR_3g093720 76.744 43 10 0 81 123 215 257 6.03e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019810.01.T01 AT3G12390 71.304 115 31 1 11 123 89 203 1.47e-42 139
MsG0480019810.01.T01 AT5G13850 68.067 119 31 1 11 122 85 203 1.07e-40 134
MsG0480019810.01.T01 AT3G49470 60.345 116 40 1 14 123 102 217 1.17e-38 129
MsG0480019810.01.T01 AT1G33040 58.475 118 43 2 11 122 91 208 2.42e-37 126
MsG0480019810.01.T01 AT4G10480 60.833 120 37 2 11 122 93 210 3.63e-36 123
MsG0480019810.01.T01 AT4G10480 60.833 120 37 2 11 122 94 211 4.25e-36 123
MsG0480019810.01.T01 AT3G49470 58.879 107 38 1 14 114 102 208 3.00e-33 116

Find 30 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGACACCTACATTATATT+TGG 0.098409 4:-27718085 MsG0480019810.01.T01:CDS
GGAGCAATACCAGAGGATTC+TGG 0.324111 4:+27717971 None:intergenic
TCGTCTACATCCTCATCTTC+TGG 0.324398 4:+27717950 None:intergenic
CTGACACCTACATTATATTT+GGG 0.330139 4:-27718084 MsG0480019810.01.T01:CDS
GCACCCGCATTGGTCAAATT+AGG 0.333932 4:+27717998 None:intergenic
GCTTTAACAGCTCTTGATCT+TGG 0.356165 4:+27717875 None:intergenic
AGACATTGTTGCTGCCATTA+TGG 0.359211 4:-27717834 MsG0480019810.01.T01:CDS
GTTGGACTCTTGAAAACATC+TGG 0.370830 4:+27718109 None:intergenic
TTAATTTGTTAGCTCCATAA+TGG 0.425574 4:+27717820 None:intergenic
CACCACCAACTCAATATCCT+TGG 0.437037 4:+27717913 None:intergenic
GCTTGAGCTTCAAGCTTCAA+TGG 0.440160 4:-27719021 MsG0480019810.01.T01:CDS
TTCTGGCTTCGCACCCGCAT+TGG 0.469966 4:+27717988 None:intergenic
TCAAGCTTCAATGGCGGAAA+CGG 0.475278 4:-27719012 MsG0480019810.01.T01:intron
TCAGGCTGCAGAGCAGTTCA+AGG 0.493163 4:-27718023 MsG0480019810.01.T01:CDS
AAAGCTCTCAAAGCTGTCAA+TGG 0.508744 4:-27717857 MsG0480019810.01.T01:CDS
ATAATGTAGGTGTCAGAAGT+TGG 0.511639 4:+27718091 None:intergenic
TCCCAAGGATATTGAGTTGG+TGG 0.515624 4:-27717915 MsG0480019810.01.T01:CDS
GATCCTAATTTGACCAATGC+GGG 0.519700 4:-27718001 MsG0480019810.01.T01:CDS
ACCACCAACTCAATATCCTT+GGG 0.531886 4:+27717914 None:intergenic
GGTGCGAAGCCAGAATCCTC+TGG 0.535151 4:-27717980 MsG0480019810.01.T01:CDS
GATGAGGATGTAGACGAGAC+TGG 0.538671 4:-27717944 MsG0480019810.01.T01:CDS
GAGTTGGTGGTGACTCAAGC+TGG 0.556994 4:-27717902 MsG0480019810.01.T01:CDS
AGCTTTCCCAAATATAATGT+AGG 0.558527 4:+27718078 None:intergenic
GAGCTCACAGCTACAAACTC+AGG 0.597975 4:-27718041 MsG0480019810.01.T01:CDS
CGAGACTGGTGTAGATCCCA+AGG 0.607645 4:-27717930 MsG0480019810.01.T01:CDS
TGGTATTGCTCCAGAAGATG+AGG 0.607813 4:-27717960 MsG0480019810.01.T01:CDS
AGATCCCAAGGATATTGAGT+TGG 0.629225 4:-27717918 MsG0480019810.01.T01:CDS
ATCTTCTGGAGCAATACCAG+AGG 0.670950 4:+27717964 None:intergenic
GGATCCTAATTTGACCAATG+CGG 0.677685 4:-27718002 MsG0480019810.01.T01:CDS
TGAGCTTCAAGCTTCAATGG+CGG 0.684568 4:-27719018 MsG0480019810.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAAAATAGTGAAAAT+AGG - Chr4:27718399-27718418 MsG0480019810.01.T01:intron 10.0%
!!! ATATTTTTTTCTTCTTCTTT+TGG - Chr4:27718654-27718673 MsG0480019810.01.T01:intron 15.0%
!!! TTTGCTTTTATTACATAATA+AGG + Chr4:27718097-27718116 None:intergenic 15.0%
!! TTGGTTAGAATAACAATAAT+TGG + Chr4:27718245-27718264 None:intergenic 20.0%
!!! AGTTCTTTTTGAAATTTCAA+TGG - Chr4:27718274-27718293 MsG0480019810.01.T01:intron 20.0%
! AATTGTCTTTCAATGTTTCT+TGG + Chr4:27718155-27718174 None:intergenic 25.0%
! TATTCTTGTATAGACTAATC+TGG + Chr4:27718529-27718548 None:intergenic 25.0%
! TGTTTCTTGGAAAGAAATAA+TGG + Chr4:27718142-27718161 None:intergenic 25.0%
! TTGATTACAGTCCTATATAT+AGG - Chr4:27717971-27717990 MsG0480019810.01.T01:CDS 25.0%
!!! AAAAAGAACTTGTTGCTATT+TGG + Chr4:27718264-27718283 None:intergenic 25.0%
!!! GTTGTTTTAGTTTCATGATA+TGG + Chr4:27717917-27717936 None:intergenic 25.0%
!!! TTAATTTGTTAGCTCCATAA+TGG + Chr4:27719032-27719051 None:intergenic 25.0%
!!! TTTATTTTTTGCATGAACTG+AGG - Chr4:27718614-27718633 MsG0480019810.01.T01:intron 25.0%
AGAGAATGACTACTATTAGA+GGG + Chr4:27718450-27718469 None:intergenic 30.0%
AGCTTTCCCAAATATAATGT+AGG + Chr4:27718774-27718793 None:intergenic 30.0%
CAAAATACATGTACATTGTC+AGG - Chr4:27718553-27718572 MsG0480019810.01.T01:intron 30.0%
CTGACACCTACATTATATTT+GGG - Chr4:27718765-27718784 MsG0480019810.01.T01:intron 30.0%
GAAGTCATTATCAAAAGATG+TGG - Chr4:27718345-27718364 MsG0480019810.01.T01:intron 30.0%
GCTCTCATTTACCTATATAT+AGG + Chr4:27717985-27718004 None:intergenic 30.0%
TAGAGAATGACTACTATTAG+AGG + Chr4:27718451-27718470 None:intergenic 30.0%
TCTGACACCTACATTATATT+TGG - Chr4:27718764-27718783 MsG0480019810.01.T01:intron 30.0%
! CTGACAATGTACATGTATTT+TGG + Chr4:27718555-27718574 None:intergenic 30.0%
!! AGAGAGTTGATAACTAATTC+TGG - Chr4:27718201-27718220 MsG0480019810.01.T01:intron 30.0%
AACGGTTAGAAAACAGAAAC+TGG - Chr4:27717855-27717874 MsG0480019810.01.T01:CDS 35.0%
AAGTTTAGACTACATCCTTC+TGG + Chr4:27718478-27718497 None:intergenic 35.0%
ATAATGTAGGTGTCAGAAGT+TGG + Chr4:27718761-27718780 None:intergenic 35.0%
GTCTAAACTTGAACATACTC+TGG - Chr4:27718488-27718507 MsG0480019810.01.T01:intron 35.0%
! CAAGTTACACAGTTAGCTTA+GGG + Chr4:27718052-27718071 None:intergenic 35.0%
! TAGGAGTACACAAGTATTAG+AGG - Chr4:27718418-27718437 MsG0480019810.01.T01:intron 35.0%
AAAGCTCTCAAAGCTGTCAA+TGG - Chr4:27718992-27719011 MsG0480019810.01.T01:intron 40.0%
ACCACCAACTCAATATCCTT+GGG + Chr4:27718938-27718957 None:intergenic 40.0%
AGACATTGTTGCTGCCATTA+TGG - Chr4:27719015-27719034 MsG0480019810.01.T01:CDS 40.0%
AGATCCCAAGGATATTGAGT+TGG - Chr4:27718931-27718950 MsG0480019810.01.T01:intron 40.0%
ATGAACTGAGGCTACAATGA+TGG - Chr4:27718626-27718645 MsG0480019810.01.T01:intron 40.0%
GATCCTAATTTGACCAATGC+GGG - Chr4:27718848-27718867 MsG0480019810.01.T01:intron 40.0%
GCTTTAACAGCTCTTGATCT+TGG + Chr4:27718977-27718996 None:intergenic 40.0%
GGATCCTAATTTGACCAATG+CGG - Chr4:27718847-27718866 MsG0480019810.01.T01:intron 40.0%
GGATCGTAACAAACTCTCTA+AGG - Chr4:27717876-27717895 MsG0480019810.01.T01:CDS 40.0%
GTCATTCTCTATTGACCAGA+AGG - Chr4:27718460-27718479 MsG0480019810.01.T01:intron 40.0%
GTTGGACTCTTGAAAACATC+TGG + Chr4:27718743-27718762 None:intergenic 40.0%
TTGAAAGTCATGCAGAACAG+TGG + Chr4:27718581-27718600 None:intergenic 40.0%
! GCAAGTTACACAGTTAGCTT+AGG + Chr4:27718053-27718072 None:intergenic 40.0%
CACCACCAACTCAATATCCT+TGG + Chr4:27718939-27718958 None:intergenic 45.0%
GCTTGAGCTTCAAGCTTCAA+TGG - Chr4:27717828-27717847 MsG0480019810.01.T01:CDS 45.0%
TCGTCTACATCCTCATCTTC+TGG + Chr4:27718902-27718921 None:intergenic 45.0%
TGAGCTTCAAGCTTCAATGG+CGG - Chr4:27717831-27717850 MsG0480019810.01.T01:CDS 45.0%
TGGTATTGCTCCAGAAGATG+AGG - Chr4:27718889-27718908 MsG0480019810.01.T01:intron 45.0%
! TCAAGCTTCAATGGCGGAAA+CGG - Chr4:27717837-27717856 MsG0480019810.01.T01:CDS 45.0%
!! ATCTTCTGGAGCAATACCAG+AGG + Chr4:27718888-27718907 None:intergenic 45.0%
!! TCCCAAGGATATTGAGTTGG+TGG - Chr4:27718934-27718953 MsG0480019810.01.T01:intron 45.0%
GAGCTCACAGCTACAAACTC+AGG - Chr4:27718808-27718827 MsG0480019810.01.T01:intron 50.0%
GATGAGGATGTAGACGAGAC+TGG - Chr4:27718905-27718924 MsG0480019810.01.T01:intron 50.0%
GGAGCAATACCAGAGGATTC+TGG + Chr4:27718881-27718900 None:intergenic 50.0%
! GCACCCGCATTGGTCAAATT+AGG + Chr4:27718854-27718873 None:intergenic 50.0%
CGAGACTGGTGTAGATCCCA+AGG - Chr4:27718919-27718938 MsG0480019810.01.T01:intron 55.0%
TCAGGCTGCAGAGCAGTTCA+AGG - Chr4:27718826-27718845 MsG0480019810.01.T01:intron 55.0%
!! GAGTTGGTGGTGACTCAAGC+TGG - Chr4:27718947-27718966 MsG0480019810.01.T01:intron 55.0%
GGTGCGAAGCCAGAATCCTC+TGG - Chr4:27718869-27718888 MsG0480019810.01.T01:intron 60.0%
!! TTCTGGCTTCGCACCCGCAT+TGG + Chr4:27718864-27718883 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 27717820 27719051 27717820 ID=MsG0480019810.01;Name=MsG0480019810.01
Chr4 mRNA 27717820 27719051 27717820 ID=MsG0480019810.01.T01;Parent=MsG0480019810.01;Name=MsG0480019810.01.T01;_AED=0.53;_eAED=0.53;_QI=0|0|0|0.5|1|1|2|0|123
Chr4 exon 27719013 27719051 27719013 ID=MsG0480019810.01.T01:exon:5848;Parent=MsG0480019810.01.T01
Chr4 exon 27717820 27718152 27717820 ID=MsG0480019810.01.T01:exon:5847;Parent=MsG0480019810.01.T01
Chr4 CDS 27719013 27719051 27719013 ID=MsG0480019810.01.T01:cds;Parent=MsG0480019810.01.T01
Chr4 CDS 27717820 27718152 27717820 ID=MsG0480019810.01.T01:cds;Parent=MsG0480019810.01.T01
Gene Sequence

>MsG0480019810.01.T01

ATGAAGAAGCTTGAGCTTCAAGCTTCAATGGCGGAAACGATCTTGTTCGTCATCTCAAAACCAGATGTTTTCAAGAGTCCAACTTCTGACACCTACATTATATTTGGGAAAGCTAAGATTGAATACTTGAGCTCACAGCTACAAACTCAGGCTGCAGAGCAGTTCAAGGATCCTAATTTGACCAATGCGGGTGCGAAGCCAGAATCCTCTGGTATTGCTCCAGAAGATGAGGATGTAGACGAGACTGGTGTAGATCCCAAGGATATTGAGTTGGTGGTGACTCAAGCTGGTGTGCCAAGATCAAGAGCTGTTAAAGCTCTCAAAGCTGTCAATGGAGACATTGTTGCTGCCATTATGGAGCTAACAAATTAA

Protein sequence

>MsG0480019810.01.T01

MKKLELQASMAETILFVISKPDVFKSPTSDTYIIFGKAKIEYLSSQLQTQAAEQFKDPNLTNAGAKPESSGIAPEDEDVDETGVDPKDIELVVTQAGVPRSRAVKALKAVNGDIVAAIMELTN*