AlfalfaGEDB | MsG0480023739.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023739.01

KEGG: -
GO: GO:0001522 GO:0003723 GO:0009451 GO:0009982
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023739.01.T01	XP_003630601.2	80.587	443	25	5	3	393	2	435	0	682

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023739.01.T01	G7LIU9	80.587	443	25	5	3	393	2	435	0.0	682

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0480023739.01	0.825627	4.228395e-54	3.875000e-51
MsG0080048773.01	MsG0480023739.01	0.827349	1.643593e-54	1.582812e-51
MsG0080049040.01	MsG0480023739.01	0.813053	3.109367e-51	2.009345e-48
MsG0080049068.01	MsG0480023739.01	0.830808	2.383536e-55	2.538814e-52
MsG0180000355.01	MsG0480023739.01	0.811309	7.465699e-51	4.605389e-48
MsG0180000785.01	MsG0480023739.01	0.823123	1.640789e-53	1.400335e-50
MsG0180001270.01	MsG0480023739.01	0.818038	2.414896e-52	1.787741e-49
MsG0180001610.01	MsG0480023739.01	0.827510	1.503604e-54	1.454599e-51
MsG0180002399.01	MsG0480023739.01	0.819399	1.185599e-52	9.113259e-50
MsG0180003353.01	MsG0480023739.01	0.856224	3.717921e-62	8.810491e-59
MsG0180003821.01	MsG0480023739.01	0.839963	1.156683e-57	1.623596e-54
MsG0180003885.01	MsG0480023739.01	0.851900	6.563660e-61	1.348116e-57
MsG0180004046.01	MsG0480023739.01	0.803599	3.228022e-49	1.628949e-46
MsG0180004103.01	MsG0480023739.01	0.821144	4.720538e-53	3.809195e-50
MsG0180004192.01	MsG0480023739.01	0.833955	3.961758e-56	4.634165e-53
MsG0180004246.01	MsG0480023739.01	0.815198	1.045124e-51	7.157982e-49
MsG0180004466.01	MsG0480023739.01	0.825793	3.861447e-54	3.555611e-51
MsG0180004493.01	MsG0480023739.01	0.816157	6.390788e-52	4.493834e-49
MsG0180005169.01	MsG0480023739.01	0.819194	1.320260e-52	1.009066e-49
MsG0180005750.01	MsG0480023739.01	0.800275	1.555366e-48	7.212454e-46
MsG0180005807.01	MsG0480023739.01	0.833165	6.235966e-56	7.123358e-53
MsG0180006031.01	MsG0480023739.01	0.829253	5.708795e-55	5.811805e-52
MsG0280010564.01	MsG0480023739.01	0.805025	1.629199e-49	8.528182e-47
MsG0380014909.01	MsG0480023739.01	0.804644	1.956891e-49	1.014340e-46
MsG0480018584.01	MsG0480023739.01	0.835588	1.537671e-56	1.888739e-53
MsG0480018850.01	MsG0480023739.01	0.802090	6.615527e-49	3.212026e-46
MsG0480018911.01	MsG0480023739.01	0.805089	1.579199e-49	8.280320e-47
MsG0480020690.01	MsG0480023739.01	0.851156	1.066013e-60	2.138025e-57
MsG0480020890.01	MsG0480023739.01	0.800660	1.298395e-48	6.079982e-46
MsG0480022355.01	MsG0480023739.01	0.811504	6.772291e-51	4.199598e-48
MsG0480022647.01	MsG0480023739.01	0.814563	1.445780e-51	9.734144e-49
MsG0480023314.01	MsG0480023739.01	0.811152	8.076454e-51	4.961761e-48
MsG0480023384.01	MsG0480023739.01	0.818288	2.119560e-52	1.579964e-49
MsG0480023739.01	MsG0480023909.01	0.810416	1.165058e-50	7.018579e-48
MsG0480023739.01	MsG0580025055.01	0.805805	1.117750e-49	5.969660e-47
MsG0480023739.01	MsG0580025668.01	0.807386	5.185677e-50	2.884414e-47
MsG0480023739.01	MsG0580025971.01	0.800358	1.495974e-48	6.951833e-46
MsG0480023739.01	MsG0580027394.01	0.817474	3.236438e-52	2.359404e-49
MsG0480023739.01	MsG0580027655.01	0.801122	1.045089e-48	4.951501e-46
MsG0480023739.01	MsG0580027826.01	0.803956	2.721817e-49	1.386167e-46
MsG0480023739.01	MsG0580029529.01	0.831857	1.316066e-55	1.446205e-52
MsG0480023739.01	MsG0680030858.01	0.809015	2.331130e-50	1.353263e-47
MsG0480023739.01	MsG0680031010.01	0.830034	3.685151e-55	3.837396e-52
MsG0480023739.01	MsG0680031939.01	0.817361	3.431881e-52	2.493987e-49
MsG0480023739.01	MsG0680032830.01	0.804934	1.701652e-49	8.887467e-47
MsG0480023739.01	MsG0680032832.01	0.816147	6.425196e-52	4.516692e-49
MsG0480023739.01	MsG0680033377.01	0.835588	1.538011e-56	1.889140e-53
MsG0480023739.01	MsG0680034064.01	0.809923	1.488534e-50	8.852589e-48
MsG0480023739.01	MsG0680035877.01	0.817964	2.508723e-52	1.853450e-49
MsG0480023739.01	MsG0780036659.01	0.810455	1.143154e-50	6.893643e-48
MsG0480023739.01	MsG0780037395.01	0.802723	4.901168e-49	2.418416e-46
MsG0480023739.01	MsG0780038784.01	0.814187	1.750698e-51	1.166733e-48
MsG0480023739.01	MsG0780039975.01	0.805975	1.029552e-49	5.522691e-47
MsG0480023739.01	MsG0780040430.01	0.805402	1.358212e-49	7.179098e-47
MsG0480023739.01	MsG0780040443.01	0.805399	1.360385e-49	7.189798e-47
MsG0480023739.01	MsG0780041138.01	0.809673	1.684243e-50	9.951065e-48
MsG0480023739.01	MsG0780041391.01	0.809089	2.247949e-50	1.307610e-47
MsG0480023739.01	MsG0780041435.01	0.803513	3.362789e-49	1.693209e-46
MsG0480023739.01	MsG0780041634.01	0.809840	1.551159e-50	9.205022e-48
MsG0480023739.01	MsG0780041673.01	0.806375	8.482855e-50	4.596778e-47
MsG0480023739.01	MsG0880041947.01	0.821492	3.923004e-53	3.196333e-50
MsG0480023739.01	MsG0880044005.01	0.821561	3.782674e-53	3.088059e-50
MsG0480023739.01	MsG0880044392.01	0.821676	3.558068e-53	2.914090e-50
MsG0480023739.01	MsG0880044487.01	0.822596	2.176967e-53	1.830544e-50
MsG0480023739.01	MsG0880044704.01	0.802028	6.811833e-49	3.302095e-46
MsG0480023739.01	MsG0880045044.01	0.811039	8.546516e-51	5.235073e-48
MsG0480023739.01	MsG0880045558.01	0.825725	4.007920e-54	3.683184e-51
MsG0480023739.01	MsG0880045722.01	0.804276	2.335127e-49	1.198974e-46
MsG0480023739.01	MsG0880046382.01	0.804591	2.006696e-49	1.038753e-46
MsG0480023739.01	MsG0880046765.01	0.822258	2.608254e-53	2.171913e-50
MsG0480023739.01	MsG0880046867.01	0.807203	5.668033e-50	3.137826e-47
MsG0480023739.01	MsG0880047394.01	0.807377	5.207043e-50	2.895676e-47
MsG0480023739.01	MsG0880047422.01	-0.815711	8.036149e-52	5.582339e-49
MsG0480023739.01	MsG0880047703.01	0.808848	2.531883e-50	1.463247e-47
MsG0380015784.01	MsG0480023739.01	0.809277	2.049137e-50	1.197963e-47
MsG0380015896.01	MsG0480023739.01	0.813249	2.816219e-51	1.829566e-48
MsG0380016284.01	MsG0480023739.01	0.819821	9.497038e-53	7.386830e-50
MsG0380016294.01	MsG0480023739.01	0.803700	3.076328e-49	1.556341e-46
MsG0380016540.01	MsG0480023739.01	0.814253	1.692909e-51	1.130235e-48
MsG0380016671.01	MsG0480023739.01	0.832553	8.854003e-56	9.930338e-53
MsG0380016864.01	MsG0480023739.01	0.804384	2.217226e-49	1.141615e-46
MsG0380017087.01	MsG0480023739.01	0.805133	1.546340e-49	8.117248e-47
MsG0380017663.01	MsG0480023739.01	0.809161	2.169049e-50	1.264179e-47
MsG0380018037.01	MsG0480023739.01	0.836564	8.691111e-57	1.099323e-53
MsG0380018069.01	MsG0480023739.01	0.811656	6.277850e-51	3.908171e-48
MsG0280006996.01	MsG0480023739.01	0.831107	2.013500e-55	2.163550e-52
MsG0280007502.01	MsG0480023739.01	0.801661	8.105371e-49	3.892559e-46
MsG0280007976.01	MsG0480023739.01	0.807558	4.766799e-50	2.663231e-47
MsG0280009256.01	MsG0480023739.01	0.801897	7.249269e-49	3.502557e-46
MsG0280009685.01	MsG0480023739.01	0.804235	2.381309e-49	1.221350e-46
MsG0280009756.01	MsG0480023739.01	0.802711	4.927305e-49	2.430546e-46
MsG0280010503.01	MsG0480023739.01	0.807473	4.968367e-50	2.769927e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023739.01.T01	MTR_8g101250	80.587	443	25	5	3	393	2	435	0.0	682
MsG0480023739.01.T01	MTR_8g101250	80.882	340	24	5	1	309	1	330	0.0	539

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023739.01.T01	AT5G35400	49.644	421	128	6	47	390	6	419	1.05e-117	348
MsG0480023739.01.T01	AT5G35400	48.246	342	114	5	47	332	6	340	3.52e-94	290
MsG0480023739.01.T01	AT5G35400	75.000	60	15	0	331	390	411	470	3.46e-15	77.4

Find 120 sgRNAs with CRISPR-Local

Find 415 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACACATATTCCTGTATTT+TGG	0.124178	4:+89260912	MsG0480023739.01.T01:three_prime_UTR
TTCTGGAGTTCCAGAAATTT+TGG	0.144575	4:+89255718	MsG0480023739.01.T01:CDS
AATGATGCTACTCCCAATTT+TGG	0.147183	4:-89260946	None:intergenic
TCAGTATTATCCAAAATTTC+TGG	0.163796	4:-89255728	None:intergenic
TAAAAGTTACTCACAGTTAT+AGG	0.196185	4:-89257960	None:intergenic
GGACTCGGTTCACCCAAAAT+TGG	0.241484	4:+89260933	MsG0480023739.01.T01:three_prime_UTR
ATTGCATCTTCAATTTCTTT+TGG	0.271658	4:-89257090	None:intergenic
GTATGCATTATTCTTCTATT+AGG	0.273717	4:+89255215	MsG0480023739.01.T01:exon
ACTCACAGTTATAGGCAATT+TGG	0.278122	4:-89257952	None:intergenic
AGCGTTCCATCTAGCGTATT+TGG	0.282194	4:-89255383	None:intergenic
TGATGCCTTGTTAAAGCTTA+TGG	0.285310	4:+89260472	MsG0480023739.01.T01:CDS
GAGGCACTGATCTAAATTAT+TGG	0.306858	4:+89260888	MsG0480023739.01.T01:three_prime_UTR
CCACTTCCCACTGCGATCTT+TGG	0.311147	4:-89255771	None:intergenic
ATCATGAAAGATTAGAGCTT+TGG	0.334643	4:+89260593	MsG0480023739.01.T01:exon
GAAGGTTGTGCTGCTGTTGC+TGG	0.345856	4:+89256042	MsG0480023739.01.T01:CDS
AAAGTGGAAGGGGAGCAATT+TGG	0.362867	4:+89260849	MsG0480023739.01.T01:three_prime_UTR
GACTCGGTTCACCCAAAATT+GGG	0.366579	4:+89260934	MsG0480023739.01.T01:three_prime_UTR
TCTTAAACTCGCCGTGGTGC+AGG	0.368122	4:+89260822	MsG0480023739.01.T01:three_prime_UTR
GTGACCGTGATGAAGGATAA+AGG	0.378057	4:-89255285	None:intergenic
ATCCCTCCAAATACGCTAGA+TGG	0.395430	4:+89255377	MsG0480023739.01.T01:CDS
AGCACCAAAGTGGCGGCAAT+GGG	0.403358	4:+89257390	MsG0480023739.01.T01:CDS
GATGTGAACATGGGCTTGAT+TGG	0.407455	4:-89261114	None:intergenic
CGGTGAGTGGGAAAATATAA+AGG	0.408298	4:-89257355	None:intergenic
CAAATTCACGATTCATTTGT+CGG	0.408720	4:+89261014	MsG0480023739.01.T01:three_prime_UTR
AATTCATGTACGCAAGGCCT+TGG	0.418528	4:+89255505	MsG0480023739.01.T01:CDS
TATTAGGAAGCACATGTTGA+TGG	0.419877	4:+89255231	MsG0480023739.01.T01:CDS
ATCAGTGCCTCCACAATTAG+AGG	0.423878	4:-89260876	None:intergenic
GTTGCTGGTCGAACAGATAA+AGG	0.423976	4:+89256057	MsG0480023739.01.T01:CDS
CTTAAACTCGCCGTGGTGCA+GGG	0.433918	4:+89260823	MsG0480023739.01.T01:three_prime_UTR
CTCCATTTGGCAGAGAAGTT+CGG	0.434689	4:-89257326	None:intergenic
ATCCGAACTTCTCTGCCAAA+TGG	0.439098	4:+89257324	MsG0480023739.01.T01:CDS
TCAACTAGACATAGGCCATC+AGG	0.443873	4:-89260533	None:intergenic
ACTCCCCATTGCCGCCACTT+TGG	0.457889	4:-89257394	None:intergenic
CAACGCCGTGACCGTGATGA+AGG	0.459020	4:-89255292	None:intergenic
AAGCACCAAAGTGGCGGCAA+TGG	0.461294	4:+89257389	MsG0480023739.01.T01:CDS
ATAAAGGTAACGCCTCCATT+TGG	0.471217	4:-89257339	None:intergenic
GCAATAAATGACGCAGCACC+AGG	0.471729	4:+89257108	MsG0480023739.01.T01:CDS
TGGTTATGCAGAATTTGAAC+GGG	0.476034	4:+89260559	MsG0480023739.01.T01:CDS
ATCTAGCGTATTTGGAGGGA+TGG	0.476956	4:-89255375	None:intergenic
CGTATTTGGAGGGATGGGTA+TGG	0.479472	4:-89255369	None:intergenic
GATGACTATCTCTTTCACTT+TGG	0.481377	4:-89255333	None:intergenic
TGTGATTCTGACAGCAAGGA+TGG	0.483744	4:+89257446	MsG0480023739.01.T01:CDS
TTTCCCACTCACCGATGGAC+AGG	0.493266	4:+89257364	MsG0480023739.01.T01:CDS
GGCAGAGAAGTTCGGATGAA+AGG	0.497782	4:-89257318	None:intergenic
GAGGGCTGTATCTGTATATG+AGG	0.504365	4:+89257137	MsG0480023739.01.T01:CDS
AATCATATGTGTTCAGCGTA+AGG	0.506719	4:+89257486	MsG0480023739.01.T01:CDS
TTCACGATTCATTTGTCGGA+GGG	0.509137	4:+89261018	MsG0480023739.01.T01:three_prime_UTR
CAACTAGACATAGGCCATCA+GGG	0.514616	4:-89260532	None:intergenic
TGACCGTGATGAAGGATAAA+GGG	0.517542	4:-89255284	None:intergenic
CGCAGCACCAGGAAAATTGA+GGG	0.519656	4:+89257119	MsG0480023739.01.T01:CDS
TCAGCGTAAGGAAGGTGAAC+CGG	0.519816	4:+89257498	MsG0480023739.01.T01:CDS
CCGTGGTGCAGGGGAGAAAG+TGG	0.519861	4:+89260833	MsG0480023739.01.T01:three_prime_UTR
ATTTGGATTCCCTCTAATTG+TGG	0.520163	4:+89260866	MsG0480023739.01.T01:three_prime_UTR
GGCCTATGTCTAGTTGATGT+TGG	0.520883	4:+89260539	MsG0480023739.01.T01:CDS
GTTCATTATGCACAGACACT+TGG	0.523107	4:+89257052	MsG0480023739.01.T01:intron
CAACAATAAGGATGTGAACA+TGG	0.528312	4:-89261124	None:intergenic
ATCACGGGCAAACATCTTGT+AGG	0.529576	4:-89257552	None:intergenic
GGCTGCAGCTTCTCTAATTG+TGG	0.529621	4:-89260438	None:intergenic
TTGATTGGATAAATAAATGA+AGG	0.530762	4:-89261099	None:intergenic
TGTTCACCATGCTCGAGCTA+TGG	0.531630	4:+89257926	MsG0480023739.01.T01:CDS
GGTGCAGGGGAGAAAGTGGA+AGG	0.531815	4:+89260837	MsG0480023739.01.T01:three_prime_UTR
GTGAACCGAGTCCAAAATAC+AGG	0.532166	4:-89260923	None:intergenic
AAAAGAACAAACTTGTTGAA+GGG	0.533238	4:-89256088	None:intergenic
ATTAGGAAGCACATGTTGAT+GGG	0.533644	4:+89255232	MsG0480023739.01.T01:CDS
TTGGTTATGCAGAATTTGAA+CGG	0.537032	4:+89260558	MsG0480023739.01.T01:CDS
TTTATCCTTCATCACGGTCA+CGG	0.537752	4:+89255287	MsG0480023739.01.T01:CDS
ACGCAGCACCAGGAAAATTG+AGG	0.539419	4:+89257118	MsG0480023739.01.T01:CDS
TGCAGGGGAGAAAGTGGAAG+GGG	0.550090	4:+89260839	MsG0480023739.01.T01:three_prime_UTR
AAACAAAACTCAACTGCTCA+AGG	0.552362	4:+89256002	MsG0480023739.01.T01:CDS
ATGCACAGACACTTGGAAGA+AGG	0.552624	4:+89257059	MsG0480023739.01.T01:intron
TTGGTGCTTATCCTGTCCAT+CGG	0.554439	4:-89257375	None:intergenic
GACAAAGGTTTGCCTATCGA+AGG	0.558005	4:+89256024	MsG0480023739.01.T01:CDS
AAATCATTGACTCGTTGCCA+AGG	0.574577	4:-89255522	None:intergenic
CAAGGCATCATCTTCAGCAC+CGG	0.575344	4:-89260459	None:intergenic
TGGATTCCCTCTAATTGTGG+AGG	0.583649	4:+89260869	MsG0480023739.01.T01:three_prime_UTR
TGGCTTCCATAGCTCGAGCA+TGG	0.584068	4:-89257932	None:intergenic
GTTCCATCTAGCGTATTTGG+AGG	0.584981	4:-89255380	None:intergenic
GTGATTCTGACAGCAAGGAT+GGG	0.588694	4:+89257447	MsG0480023739.01.T01:CDS
GCAGATGGTTAGAGTGCTTG+TGG	0.588706	4:+89260412	MsG0480023739.01.T01:intron
TATCCTGTCCATCGGTGAGT+GGG	0.593687	4:-89257367	None:intergenic
ATATGTGTTCAGCGTAAGGA+AGG	0.595166	4:+89257490	MsG0480023739.01.T01:CDS
TTCCATCTAGCGTATTTGGA+GGG	0.595576	4:-89255379	None:intergenic
TTGAGTGAGCAAAGTGAAAG+CGG	0.595601	4:+89260697	MsG0480023739.01.T01:three_prime_UTR
ACAATTAGAGAAGCTGCAGC+CGG	0.599406	4:+89260440	MsG0480023739.01.T01:CDS
TTAAACTCGCCGTGGTGCAG+GGG	0.600676	4:+89260824	MsG0480023739.01.T01:three_prime_UTR
ACGGCGTTGTAATTATTCGA+TGG	0.601058	4:+89255306	MsG0480023739.01.T01:CDS
TGAACAAAACACTCTGTTGG+TGG	0.601474	4:-89257909	None:intergenic
CGAACTTCTCTGCCAAATGG+AGG	0.604545	4:+89257327	MsG0480023739.01.T01:CDS
TTTCCCTTTATCCTTCATCA+CGG	0.605108	4:+89255281	MsG0480023739.01.T01:CDS
AATACTGAGTTCGAAAGTGA+TGG	0.606706	4:+89255743	MsG0480023739.01.T01:CDS
TTATCCTGTCCATCGGTGAG+TGG	0.607517	4:-89257368	None:intergenic
TGAAAGCGGATGCATGCACA+AGG	0.613310	4:+89260711	MsG0480023739.01.T01:three_prime_UTR
TCTAGCGTATTTGGAGGGAT+GGG	0.615798	4:-89255374	None:intergenic
GAAGAATAATGCATACCCTA+CGG	0.616986	4:-89255208	None:intergenic
GAGGCAAAGAGAAGTTGTCA+CGG	0.619172	4:-89255560	None:intergenic
AACAATAAGGATGTGAACAT+GGG	0.619917	4:-89261123	None:intergenic
GTGTATCCAAAGATCGCAGT+GGG	0.622172	4:+89255765	MsG0480023739.01.T01:CDS
GCTTCGAATTCATGTACGCA+AGG	0.624809	4:+89255499	MsG0480023739.01.T01:CDS
ACTCAACTGCTCAAGGACAA+AGG	0.624888	4:+89256009	MsG0480023739.01.T01:CDS
AACTAGACATAGGCCATCAG+GGG	0.624908	4:-89260531	None:intergenic
AATACGCTAGATGGAACGCT+AGG	0.637091	4:+89255386	MsG0480023739.01.T01:CDS
ATTCACGATTCATTTGTCGG+AGG	0.641711	4:+89261017	MsG0480023739.01.T01:three_prime_UTR
ACAGCAGCACAACCTTCGAT+AGG	0.641899	4:-89256036	None:intergenic
AGCTTATGGATGCTACGTGT+AGG	0.642304	4:+89260486	MsG0480023739.01.T01:CDS
GTGCAGGGGAGAAAGTGGAA+GGG	0.642313	4:+89260838	MsG0480023739.01.T01:three_prime_UTR
TCAGTGCCTCCACAATTAGA+GGG	0.645042	4:-89260875	None:intergenic
CAGGATAAGCACCAAAGTGG+CGG	0.647562	4:+89257383	MsG0480023739.01.T01:CDS
AACCAACATCAACTAGACAT+AGG	0.649817	4:-89260541	None:intergenic
AGCATTGTAGAAGTGTCACT+TGG	0.651614	4:+89255964	MsG0480023739.01.T01:intron
AGCATCCATAAGCTTTAACA+AGG	0.652057	4:-89260477	None:intergenic
CATTGCTCTTAAACTCGCCG+TGG	0.663532	4:+89260816	MsG0480023739.01.T01:three_prime_UTR
CCACTTTCTCCCCTGCACCA+CGG	0.667847	4:-89260833	None:intergenic
CGGCGAGTTTAAGAGCAATG+TGG	0.670481	4:-89260813	None:intergenic
GGACAGGATAAGCACCAAAG+TGG	0.670924	4:+89257380	MsG0480023739.01.T01:CDS
AATTTGTGATTCTGACAGCA+AGG	0.676048	4:+89257442	MsG0480023739.01.T01:CDS
GTTGTTGTGTCTGAACAAGA+AGG	0.682813	4:-89255257	None:intergenic
GCACCAAAGTGGCGGCAATG+GGG	0.683256	4:+89257391	MsG0480023739.01.T01:CDS
TGGTGAACAAAACACTCTGT+TGG	0.686678	4:-89257912	None:intergenic
GGTGTATCCAAAGATCGCAG+TGG	0.708490	4:+89255764	MsG0480023739.01.T01:CDS
ACTAGACATAGGCCATCAGG+GGG	0.716575	4:-89260530	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATATATATATATATAT+GGG	+	Chr4:89256424-89256443	MsG0480023739.01.T01:intron	0.0%
!!	TATATATATATATATATATA+TGG	+	Chr4:89256425-89256444	MsG0480023739.01.T01:intron	0.0%
!!	TATATATATATATATATATA+TGG	-	Chr4:89256444-89256425	None:intergenic	0.0%
!!	TAAAATTGTTATGATAAATA+AGG	+	Chr4:89256845-89256864	MsG0480023739.01.T01:intron	10.0%
!!	TATAAAAAAAAAAAAAAGGA+GGG	+	Chr4:89260779-89260798	MsG0480023739.01.T01:three_prime_UTR	10.0%
!!	TGCTTATAAAAAAAAAAAAA+AGG	+	Chr4:89260775-89260794	MsG0480023739.01.T01:three_prime_UTR	10.0%
!!	TTATAAAAAAAAAAAAAAGG+AGG	+	Chr4:89260778-89260797	MsG0480023739.01.T01:three_prime_UTR	10.0%
!!!	TTTTTTTTTTTTTATAAGCA+CGG	-	Chr4:89260775-89260794	None:intergenic	10.0%
!!	AAATTATAGTCATATTCATT+AGG	+	Chr4:89258395-89258414	MsG0480023739.01.T01:intron	15.0%
!!	AACAATAATAATGTGAATTA+TGG	+	Chr4:89257282-89257301	MsG0480023739.01.T01:intron	15.0%
!!	ATAAAAAAAAAAAAAAGGAG+GGG	+	Chr4:89260780-89260799	MsG0480023739.01.T01:three_prime_UTR	15.0%
!!	ATTTATGAATGACAAATATA+TGG	+	Chr4:89258748-89258767	MsG0480023739.01.T01:intron	15.0%
!!	TACATTTCTCAAAAAAAAAA+TGG	-	Chr4:89258668-89258687	None:intergenic	15.0%
!!	TCTTAATATTAGAAAGATAA+CGG	-	Chr4:89260149-89260168	None:intergenic	15.0%
!!!	AAACAAATGAATCATTTTAA+AGG	+	Chr4:89260182-89260201	MsG0480023739.01.T01:intron	15.0%
!!!	ATTCTTTTTCTTTATATTCA+TGG	-	Chr4:89257221-89257240	None:intergenic	15.0%
!!!	GAATTTAGAATAACAATATT+GGG	-	Chr4:89258049-89258068	None:intergenic	15.0%
!!!	TATATTTTATCCATTTGTAA+TGG	+	Chr4:89260229-89260248	MsG0480023739.01.T01:intron	15.0%
!!!	TCATTTTATTTTACATTACA+GGG	+	Chr4:89255473-89255492	MsG0480023739.01.T01:intron	15.0%
!!!	TTCATTTTATTTTACATTAC+AGG	+	Chr4:89255472-89255491	MsG0480023739.01.T01:intron	15.0%
!!	AAATTTCTGAATCATACATT+AGG	+	Chr4:89259112-89259131	MsG0480023739.01.T01:intron	20.0%
!!	AAGACATTGATATTAAGAAT+AGG	-	Chr4:89256127-89256146	None:intergenic	20.0%
!!	AATTGAAATGTAGTTAGTTA+GGG	-	Chr4:89255610-89255629	None:intergenic	20.0%
!!	AGAAAAAGAATCAGAAAAAA+TGG	+	Chr4:89257230-89257249	MsG0480023739.01.T01:intron	20.0%
!!	AGAAAGAGAGAAAAAAATAA+AGG	+	Chr4:89256878-89256897	MsG0480023739.01.T01:intron	20.0%
!!	AGAAGAACAAAAAAAAACTA+TGG	-	Chr4:89260265-89260284	None:intergenic	20.0%
!!	AGACATTGATATTAAGAATA+GGG	-	Chr4:89256126-89256145	None:intergenic	20.0%
!!	ATAATAATGTGAATTATGGT+AGG	+	Chr4:89257286-89257305	MsG0480023739.01.T01:intron	20.0%
!!	ATATGTTATGTCTAACAATA+TGG	+	Chr4:89256226-89256245	MsG0480023739.01.T01:intron	20.0%
!!	ATATTGTTAGACATAACATA+TGG	-	Chr4:89256227-89256246	None:intergenic	20.0%
!!	ATTTCTCAAAAAAAAAATGG+AGG	-	Chr4:89258665-89258684	None:intergenic	20.0%
!!	CAATGTGTATGTAAAAAAAA+AGG	-	Chr4:89259020-89259039	None:intergenic	20.0%
!!	GAAGAACAAAAAAAAACTAT+GGG	-	Chr4:89260264-89260283	None:intergenic	20.0%
!!	GAATCAAATCTTAAATTGTA+TGG	+	Chr4:89257705-89257724	MsG0480023739.01.T01:intron	20.0%
!!	TAATTGAAATGTAGTTAGTT+AGG	-	Chr4:89255611-89255630	None:intergenic	20.0%
!!	TAGAAAGTGAATAATTAAGA+AGG	-	Chr4:89255662-89255681	None:intergenic	20.0%
!!	TTGATTGGATAAATAAATGA+AGG	-	Chr4:89261102-89261121	None:intergenic	20.0%
!!	TTTCTCAAAAAAAAAATGGA+GGG	-	Chr4:89258664-89258683	None:intergenic	20.0%
!!!	AACAATTTTACCATCAAATT+AGG	-	Chr4:89256835-89256854	None:intergenic	20.0%
!!!	AAGACCTATATTTTTAGAAA+TGG	+	Chr4:89258587-89258606	MsG0480023739.01.T01:intron	20.0%
!!!	AAGTGTATACTGTTTTTTTT+TGG	+	Chr4:89258849-89258868	MsG0480023739.01.T01:intron	20.0%
!!!	AGTGTATACTGTTTTTTTTT+GGG	+	Chr4:89258850-89258869	MsG0480023739.01.T01:intron	20.0%
!!!	ATTTTTGTAAATGACTCTAA+AGG	+	Chr4:89260651-89260670	MsG0480023739.01.T01:three_prime_UTR	20.0%
!!!	CGAATTTAGAATAACAATAT+TGG	-	Chr4:89258050-89258069	None:intergenic	20.0%
!!!	TATGTTATTTTTTGTATGAC+AGG	+	Chr4:89259042-89259061	MsG0480023739.01.T01:intron	20.0%
!!!	TATGTTTTTTATTTCCTTAG+AGG	+	Chr4:89259833-89259852	MsG0480023739.01.T01:intron	20.0%
!!!	TGTGACAGTTTTATATATAA+TGG	+	Chr4:89257419-89257438	MsG0480023739.01.T01:CDS	20.0%
!!!	TTTTTGTAAATGACTCTAAA+GGG	+	Chr4:89260652-89260671	MsG0480023739.01.T01:three_prime_UTR	20.0%
!!!	TTTTTTTTTGAGAAATGTAG+AGG	+	Chr4:89258669-89258688	MsG0480023739.01.T01:intron	20.0%
!	AAAATTCTTCTTTCCTATCA+CGG	+	Chr4:89255809-89255828	MsG0480023739.01.T01:intron	25.0%
!	AAGATAAGCTAGAAATAAAG+TGG	-	Chr4:89258321-89258340	None:intergenic	25.0%
!	AAGTGAATAATTAAGAAGGA+AGG	-	Chr4:89255658-89255677	None:intergenic	25.0%
!	AATTAAGAAGGAAGGATAAA+GGG	-	Chr4:89255650-89255669	None:intergenic	25.0%
!	ACACCTTCATAAAAATTAGT+TGG	-	Chr4:89256548-89256567	None:intergenic	25.0%
!	CAAAAAACTCTATAAAACCA+AGG	-	Chr4:89259690-89259709	None:intergenic	25.0%
!	CATGTCGCATATTATTTAAA+AGG	-	Chr4:89257169-89257188	None:intergenic	25.0%
!	CTACACTGTTTAATTCATAT+TGG	+	Chr4:89255914-89255933	MsG0480023739.01.T01:intron	25.0%
!	GCAAAGTTATAACTAAAAAG+TGG	+	Chr4:89256574-89256593	MsG0480023739.01.T01:intron	25.0%
!	GGATATGTTTAGTGATTTAA+TGG	-	Chr4:89258300-89258319	None:intergenic	25.0%
!	GGTATAGTAAAATATTTCAG+AGG	-	Chr4:89259517-89259536	None:intergenic	25.0%
!	GTACATTTAACCTAATTTGA+TGG	+	Chr4:89256822-89256841	MsG0480023739.01.T01:intron	25.0%
!	GTAGCAATTCATTTATAAGA+AGG	-	Chr4:89256163-89256182	None:intergenic	25.0%
!	GTATGCATTATTCTTCTATT+AGG	+	Chr4:89255215-89255234	MsG0480023739.01.T01:exon	25.0%
!	GTTATGATAAATAAGGATTG+AGG	+	Chr4:89256852-89256871	MsG0480023739.01.T01:intron	25.0%
!	TAAAAGTTACTCACAGTTAT+AGG	-	Chr4:89257963-89257982	None:intergenic	25.0%
!	TAATTAAGAAGGAAGGATAA+AGG	-	Chr4:89255651-89255670	None:intergenic	25.0%
!	TACTTGAATTTCTATCTTTG+TGG	+	Chr4:89257607-89257626	MsG0480023739.01.T01:intron	25.0%
!	TCAGTATTATCCAAAATTTC+TGG	-	Chr4:89255731-89255750	None:intergenic	25.0%
!	TCATGTAACGACAAATATTA+TGG	-	Chr4:89259420-89259439	None:intergenic	25.0%
!	TGATATTAAGAATAGGGAAA+TGG	-	Chr4:89256120-89256139	None:intergenic	25.0%
!	TTACATGATTTATATGTCGT+TGG	+	Chr4:89259432-89259451	MsG0480023739.01.T01:intron	25.0%
!	TTAGTGATTTAATGGAACTT+GGG	-	Chr4:89258292-89258311	None:intergenic	25.0%
!	TTCTGCTTAAATCATATGAA+AGG	-	Chr4:89258353-89258372	None:intergenic	25.0%
!	TTGTTTATGCGACTATTATT+TGG	+	Chr4:89257683-89257702	MsG0480023739.01.T01:intron	25.0%
!	TTTAGTGATTTAATGGAACT+TGG	-	Chr4:89258293-89258312	None:intergenic	25.0%
!	TTTATACACTCAAACCATTA+TGG	+	Chr4:89258636-89258655	MsG0480023739.01.T01:intron	25.0%
!	TTTGTTAGAAATGCTAGAAA+AGG	+	Chr4:89259082-89259101	MsG0480023739.01.T01:intron	25.0%
!!	AATAGCATAATTTGCATGTT+TGG	-	Chr4:89259361-89259380	None:intergenic	25.0%
!!	ACACCAACTAATTTTTATGA+AGG	+	Chr4:89256542-89256561	MsG0480023739.01.T01:intron	25.0%
!!	ACCAAGAAATTTTCTATTGT+TGG	+	Chr4:89259534-89259553	MsG0480023739.01.T01:intron	25.0%
!!	ATACTTTCACATCATACTTT+TGG	+	Chr4:89257642-89257661	MsG0480023739.01.T01:intron	25.0%
!!	ATATGGAAGTTTGTTTATGA+TGG	+	Chr4:89258765-89258784	MsG0480023739.01.T01:intron	25.0%
!!	ATGGTACTTTTGATGAAATA+GGG	-	Chr4:89256656-89256675	None:intergenic	25.0%
!!	ATTGCATCTTCAATTTCTTT+TGG	-	Chr4:89257093-89257112	None:intergenic	25.0%
!!	ATTTTATCCATTTGTAATGG+AGG	+	Chr4:89260232-89260251	MsG0480023739.01.T01:intron	25.0%
!!	CCAAGAAATTTTCTATTGTT+GGG	+	Chr4:89259535-89259554	MsG0480023739.01.T01:intron	25.0%
!!	CTGAAAATACATTTTTGTCA+AGG	-	Chr4:89259487-89259506	None:intergenic	25.0%
!!	GTCACCATTTCTAAAAATAT+AGG	-	Chr4:89258594-89258613	None:intergenic	25.0%
!!	GTGTACAAAAATAAAAGTGT+TGG	-	Chr4:89259918-89259937	None:intergenic	25.0%
!!	TAACACATATTCCTGTATTT+TGG	+	Chr4:89260912-89260931	MsG0480023739.01.T01:three_prime_UTR	25.0%
!!	TACTTTCACATCATACTTTT+GGG	+	Chr4:89257643-89257662	MsG0480023739.01.T01:intron	25.0%
!!	TATGACTATAATTTGAAGCT+TGG	-	Chr4:89258389-89258408	None:intergenic	25.0%
!!	TATGGTACTTTTGATGAAAT+AGG	-	Chr4:89256657-89256676	None:intergenic	25.0%
!!	TCTCTATTTCTCAAAAGAAT+GGG	+	Chr4:89258533-89258552	MsG0480023739.01.T01:intron	25.0%
!!!	AAAAAGAACAAACTTGTTGA+AGG	-	Chr4:89256092-89256111	None:intergenic	25.0%
!!!	AAAAGAACAAACTTGTTGAA+GGG	-	Chr4:89256091-89256110	None:intergenic	25.0%
!!!	ATATTTTTAGAAATGGTGAC+AGG	+	Chr4:89258594-89258613	MsG0480023739.01.T01:intron	25.0%
!!!	GTGTATACTGTTTTTTTTTG+GGG	+	Chr4:89258851-89258870	MsG0480023739.01.T01:intron	25.0%
!!!	TCTCTCTTTTAGAATGATAA+AGG	-	Chr4:89260076-89260095	None:intergenic	25.0%
!!!	TGTATACTGTTTTTTTTTGG+GGG	+	Chr4:89258852-89258871	MsG0480023739.01.T01:intron	25.0%
!!!	TTAGTTTTTCACATATGTGA+TGG	-	Chr4:89260750-89260769	None:intergenic	25.0%
!!!	TTATTTTTGTACACTAACAC+TGG	+	Chr4:89259924-89259943	MsG0480023739.01.T01:intron	25.0%
!!!	TTTTATCCATTTGTAATGGA+GGG	+	Chr4:89260233-89260252	MsG0480023739.01.T01:intron	25.0%
	ATCATGAAAGATTAGAGCTT+TGG	+	Chr4:89260593-89260612	MsG0480023739.01.T01:exon	30.0%
	CAAACTTGAATACTGTTCAA+AGG	+	Chr4:89255861-89255880	MsG0480023739.01.T01:intron	30.0%
	CAAATTCACGATTCATTTGT+CGG	+	Chr4:89261014-89261033	MsG0480023739.01.T01:three_prime_UTR	30.0%
	CATAGACCATCTCTAATAAT+TGG	+	Chr4:89260327-89260346	MsG0480023739.01.T01:intron	30.0%
	CCCAACAATAGAAAATTTCT+TGG	-	Chr4:89259538-89259557	None:intergenic	30.0%
	CTAAAAGACACCTTCATAAA+AGG	-	Chr4:89256493-89256512	None:intergenic	30.0%
	GTGCAATGTGAATTATATTG+AGG	-	Chr4:89258825-89258844	None:intergenic	30.0%
	GTTTGTTTATGATGGTTTCT+TGG	+	Chr4:89258773-89258792	MsG0480023739.01.T01:intron	30.0%
	TAAATGGTCAAGATGTGTAA+TGG	-	Chr4:89259279-89259298	None:intergenic	30.0%
	TCATATACAGACATAGAAGA+GGG	+	Chr4:89256250-89256269	MsG0480023739.01.T01:intron	30.0%
	TGATTTAAGCAGAACATTAC+TGG	+	Chr4:89258359-89258378	MsG0480023739.01.T01:intron	30.0%
	TTAGACATAACATATGGTTG+TGG	-	Chr4:89256221-89256240	None:intergenic	30.0%
	TTGGTTATGCAGAATTTGAA+CGG	+	Chr4:89260558-89260577	MsG0480023739.01.T01:CDS	30.0%
	TTTCCTTCAGCAACAAATAA+TGG	+	Chr4:89259215-89259234	MsG0480023739.01.T01:intron	30.0%
!	AATCAGAAAAAATGGTTTGC+TGG	+	Chr4:89257238-89257257	MsG0480023739.01.T01:intron	30.0%
!	ACTTTCACATCATACTTTTG+GGG	+	Chr4:89257644-89257663	MsG0480023739.01.T01:intron	30.0%
!	ATAGTTTCTAGACAGCAAAA+CGG	+	Chr4:89258912-89258931	MsG0480023739.01.T01:intron	30.0%
!	ATTCCATTATTTGTTGCTGA+AGG	-	Chr4:89259221-89259240	None:intergenic	30.0%
!	CAAATTATGCTATTTGTCCA+TGG	+	Chr4:89259367-89259386	MsG0480023739.01.T01:intron	30.0%
!	CAGCTAGCATTTAAGATATA+TGG	+	Chr4:89259173-89259192	MsG0480023739.01.T01:intron	30.0%
!	CTCTCTATTTCTCAAAAGAA+TGG	+	Chr4:89258532-89258551	MsG0480023739.01.T01:intron	30.0%
!	CTTTGAACAGTATTCAAGTT+TGG	-	Chr4:89255863-89255882	None:intergenic	30.0%
!	GCTAATCAAATTTTTGCAGA+TGG	+	Chr4:89260397-89260416	MsG0480023739.01.T01:intron	30.0%
!	TTCTTGAAGCTTTTGTATCA+CGG	-	Chr4:89257571-89257590	None:intergenic	30.0%
!!	ATATTCCTGTATTTTGGACT+CGG	+	Chr4:89260918-89260937	MsG0480023739.01.T01:three_prime_UTR	30.0%
!!	CATTCTTTTGAGAAATAGAG+AGG	-	Chr4:89258534-89258553	None:intergenic	30.0%
!!	TGCTGATACTACTTTTAAGA+GGG	+	Chr4:89257828-89257847	MsG0480023739.01.T01:intron	30.0%
!!	TGTTGCTAACACTCTAAAAT+TGG	-	Chr4:89259889-89259908	None:intergenic	30.0%
!!!	GTTCTTTTTTTGCACTTTGT+CGG	+	Chr4:89256998-89257017	MsG0480023739.01.T01:intron	30.0%
!!!	TTTTTTTTTGGGGGTACTTT+GGG	+	Chr4:89258861-89258880	MsG0480023739.01.T01:intron	30.0%
	AAAAACAGATTCTCGTAAGC+AGG	+	Chr4:89258186-89258205	MsG0480023739.01.T01:intron	35.0%
	AAAACAGATTCTCGTAAGCA+GGG	+	Chr4:89258187-89258206	MsG0480023739.01.T01:intron	35.0%
	AAACAAAACTCAACTGCTCA+AGG	+	Chr4:89256002-89256021	MsG0480023739.01.T01:CDS	35.0%
	AAAGTGGAAATCACACCTTA+AGG	+	Chr4:89256590-89256609	MsG0480023739.01.T01:intron	35.0%
	AACCAACATCAACTAGACAT+AGG	-	Chr4:89260544-89260563	None:intergenic	35.0%
	AATACTGAGTTCGAAAGTGA+TGG	+	Chr4:89255743-89255762	MsG0480023739.01.T01:CDS	35.0%
	AATCATATGTGTTCAGCGTA+AGG	+	Chr4:89257486-89257505	MsG0480023739.01.T01:CDS	35.0%
	AATTCCTATTGTCACTCAGA+AGG	-	Chr4:89259635-89259654	None:intergenic	35.0%
	AATTTGCATGTTTGGTTGGT+AGG	-	Chr4:89259353-89259372	None:intergenic	35.0%
	AATTTGTGATTCTGACAGCA+AGG	+	Chr4:89257442-89257461	MsG0480023739.01.T01:CDS	35.0%
	ACATCTTTGTTCACACAGTT+CGG	-	Chr4:89256724-89256743	None:intergenic	35.0%
	ACCTGAGATTGAAAAAGAAG+AGG	-	Chr4:89255582-89255601	None:intergenic	35.0%
	ACGAAACTATAGCAACTCTA+TGG	+	Chr4:89256676-89256695	MsG0480023739.01.T01:intron	35.0%
	ACTCACAGTTATAGGCAATT+TGG	-	Chr4:89257955-89257974	None:intergenic	35.0%
	AGAGTTGCTATAGTTTCGTA+TGG	-	Chr4:89256675-89256694	None:intergenic	35.0%
	ATTGCTAAAAGACACCTTCA+TGG	+	Chr4:89256618-89256637	MsG0480023739.01.T01:intron	35.0%
	ATTGGAGAAGCAAAAACCTT+CGG	-	Chr4:89259871-89259890	None:intergenic	35.0%
	ATTTGGATTCCCTCTAATTG+TGG	+	Chr4:89260866-89260885	MsG0480023739.01.T01:three_prime_UTR	35.0%
	CAATCCATTTCAATGCAGAT+TGG	-	Chr4:89259304-89259323	None:intergenic	35.0%
	CACATGCCAATTATTAGAGA+TGG	-	Chr4:89260336-89260355	None:intergenic	35.0%
	CATATACAGACATAGAAGAG+GGG	+	Chr4:89256251-89256270	MsG0480023739.01.T01:intron	35.0%
	CATCATCATCATCATCATTC+TGG	+	Chr4:89255701-89255720	MsG0480023739.01.T01:CDS	35.0%
	CTCATATACAGACATAGAAG+AGG	+	Chr4:89256249-89256268	MsG0480023739.01.T01:intron	35.0%
	GATGACTATCTCTTTCACTT+TGG	-	Chr4:89255336-89255355	None:intergenic	35.0%
	GCATAATGAACCCTAGATTA+GGG	-	Chr4:89257043-89257062	None:intergenic	35.0%
	GCTTATCATCAGAATAGAGA+GGG	+	Chr4:89258447-89258466	MsG0480023739.01.T01:intron	35.0%
	GGGCAACAAAAAAGAGATAA+AGG	-	Chr4:89258029-89258048	None:intergenic	35.0%
	GTAGACAAAACAGAATCCAT+AGG	-	Chr4:89258265-89258284	None:intergenic	35.0%
	GTCCAAGTAAACACTGAATA+GGG	+	Chr4:89258476-89258495	MsG0480023739.01.T01:intron	35.0%
	TAAAAGTAGTATCAGCACCA+AGG	-	Chr4:89257825-89257844	None:intergenic	35.0%
	TAAACGACTAAACTCAGTGA+AGG	-	Chr4:89257861-89257880	None:intergenic	35.0%
	TAGAAATGCTAGAAAAGGAC+TGG	+	Chr4:89259087-89259106	MsG0480023739.01.T01:intron	35.0%
	TAGTCCAATCTGCATTGAAA+TGG	+	Chr4:89259297-89259316	MsG0480023739.01.T01:intron	35.0%
	TCAATGCAGATTGGACTAAA+TGG	-	Chr4:89259295-89259314	None:intergenic	35.0%
	TCCAAGTAAACACTGAATAG+GGG	+	Chr4:89258477-89258496	MsG0480023739.01.T01:intron	35.0%
	TCTGTTAATATCGAAGTCCA+TGG	-	Chr4:89259387-89259406	None:intergenic	35.0%
	TGCATAATGAACCCTAGATT+AGG	-	Chr4:89257044-89257063	None:intergenic	35.0%
	TGCTTATCATCAGAATAGAG+AGG	+	Chr4:89258446-89258465	MsG0480023739.01.T01:intron	35.0%
	TGGTTATGCAGAATTTGAAC+GGG	+	Chr4:89260559-89260578	MsG0480023739.01.T01:CDS	35.0%
	TGTCCAAGTAAACACTGAAT+AGG	+	Chr4:89258475-89258494	MsG0480023739.01.T01:intron	35.0%
	TTACTGTCATTCTGTTTCAG+TGG	+	Chr4:89257885-89257904	MsG0480023739.01.T01:intron	35.0%
	TTGCTAAAAGACACCTTCAT+GGG	+	Chr4:89256619-89256638	MsG0480023739.01.T01:intron	35.0%
	TTTATGATGGTTTCTTGGAC+CGG	+	Chr4:89258778-89258797	MsG0480023739.01.T01:intron	35.0%
	TTTCCCTTTATCCTTCATCA+CGG	+	Chr4:89255281-89255300	MsG0480023739.01.T01:CDS	35.0%
!	AATGATGCTACTCCCAATTT+TGG	-	Chr4:89260949-89260968	None:intergenic	35.0%
!	AGCATCCATAAGCTTTAACA+AGG	-	Chr4:89260480-89260499	None:intergenic	35.0%
!	ATGATGATGATGATGAGGAA+GGG	-	Chr4:89255690-89255709	None:intergenic	35.0%
!	ATGATGCTACTCCCAATTTT+GGG	-	Chr4:89260948-89260967	None:intergenic	35.0%
!	ATTAGGAAGCACATGTTGAT+GGG	+	Chr4:89255232-89255251	MsG0480023739.01.T01:CDS	35.0%
!	CTTCCAATTTTTGCAAAAGC+CGG	-	Chr4:89257520-89257539	None:intergenic	35.0%
!	GCATAATTTGCATGTTTGGT+TGG	-	Chr4:89259357-89259376	None:intergenic	35.0%
!	TAGATCTCACCGTTTTATGT+GGG	-	Chr4:89259988-89260007	None:intergenic	35.0%
!	TATTAGGAAGCACATGTTGA+TGG	+	Chr4:89255231-89255250	MsG0480023739.01.T01:CDS	35.0%
!	TCTTGAAGCTTTTGTATCAC+GGG	-	Chr4:89257570-89257589	None:intergenic	35.0%
!	TGATGATGATGATGATGATG+AGG	-	Chr4:89255695-89255714	None:intergenic	35.0%
!	TGATGCCTTGTTAAAGCTTA+TGG	+	Chr4:89260472-89260491	MsG0480023739.01.T01:CDS	35.0%
!	TTTTAGCAATCCACACCTTA+AGG	-	Chr4:89256608-89256627	None:intergenic	35.0%
!!	GAGGCACTGATCTAAATTAT+TGG	+	Chr4:89260888-89260907	MsG0480023739.01.T01:three_prime_UTR	35.0%
!!	GCTGATACTACTTTTAAGAG+GGG	+	Chr4:89257829-89257848	MsG0480023739.01.T01:intron	35.0%
!!	GTGCTGATACTACTTTTAAG+AGG	+	Chr4:89257827-89257846	MsG0480023739.01.T01:intron	35.0%
!!	TTTATGTTTTGCTGTGCTTG+TGG	+	Chr4:89258094-89258113	MsG0480023739.01.T01:intron	35.0%
!!!	GTTTTTTTTTGGGGGTACTT+TGG	+	Chr4:89258860-89258879	MsG0480023739.01.T01:intron	35.0%
!!!	TTCTGGAGTTCCAGAAATTT+TGG	+	Chr4:89255718-89255737	MsG0480023739.01.T01:CDS	35.0%
	AAAAATGGAGGGCTCCATAA+TGG	-	Chr4:89258653-89258672	None:intergenic	40.0%
	AATGAACCCTAGATTAGGGA+CGG	-	Chr4:89257039-89257058	None:intergenic	40.0%
	ACATTGTTGATCCAGAGACA+TGG	+	Chr4:89258229-89258248	MsG0480023739.01.T01:intron	40.0%
	ACGGCGTTGTAATTATTCGA+TGG	+	Chr4:89255306-89255325	MsG0480023739.01.T01:CDS	40.0%
	AGAACAGGTCCCACATAAAA+CGG	+	Chr4:89259976-89259995	MsG0480023739.01.T01:intron	40.0%
	AGGAAAATGGAGAAGAAACG+AGG	+	Chr4:89259758-89259777	MsG0480023739.01.T01:intron	40.0%
	AGTAAACACTGAATAGGGGA+AGG	+	Chr4:89258481-89258500	MsG0480023739.01.T01:intron	40.0%
	ATAAAGGTAACGCCTCCATT+TGG	-	Chr4:89257342-89257361	None:intergenic	40.0%
	ATATGTGTTCAGCGTAAGGA+AGG	+	Chr4:89257490-89257509	MsG0480023739.01.T01:CDS	40.0%
	ATTCACGATTCATTTGTCGG+AGG	+	Chr4:89261017-89261036	MsG0480023739.01.T01:three_prime_UTR	40.0%
	ATTTCACCTGTGACATTGCT+AGG	+	Chr4:89259738-89259757	MsG0480023739.01.T01:intron	40.0%
	CGGTGAGTGGGAAAATATAA+AGG	-	Chr4:89257358-89257377	None:intergenic	40.0%
	CTAGACAGCAAAACGGAAAA+AGG	+	Chr4:89258919-89258938	MsG0480023739.01.T01:intron	40.0%
	CTGTGACATTGCTAGGAAAA+TGG	+	Chr4:89259745-89259764	MsG0480023739.01.T01:intron	40.0%
	GCAAAAACCTTCGGAGAATA+TGG	-	Chr4:89259862-89259881	None:intergenic	40.0%
	GCATAACCCTCCATTACAAA+TGG	-	Chr4:89260242-89260261	None:intergenic	40.0%
	GGAAAATGGAGAAGAAACGA+GGG	+	Chr4:89259759-89259778	MsG0480023739.01.T01:intron	40.0%
	GGAGAATATGGTTTCCTCTA+AGG	-	Chr4:89259850-89259869	None:intergenic	40.0%
	GTCTGTGCATTCTTCAGAAA+AGG	-	Chr4:89258702-89258721	None:intergenic	40.0%
	GTTCATTATGCACAGACACT+TGG	+	Chr4:89257052-89257071	MsG0480023739.01.T01:intron	40.0%
	GTTGTTGTGTCTGAACAAGA+AGG	-	Chr4:89255260-89255279	None:intergenic	40.0%
	TACGCCAGAAACTTTAGTGA+TGG	-	Chr4:89258565-89258584	None:intergenic	40.0%
	TAGCAATGTCACAGGTGAAA+TGG	-	Chr4:89259739-89259758	None:intergenic	40.0%
	TATGACTAACCAACTGACAG+AGG	+	Chr4:89256963-89256982	MsG0480023739.01.T01:intron	40.0%
	TCAGCACCAAGGAAATATGA+AGG	-	Chr4:89257814-89257833	None:intergenic	40.0%
	TCCCCTATTCAGTGTTTACT+TGG	-	Chr4:89258481-89258500	None:intergenic	40.0%
	TGAACAAAACACTCTGTTGG+TGG	-	Chr4:89257912-89257931	None:intergenic	40.0%
	TGACAGGTGTTTCGTATTAC+TGG	+	Chr4:89258610-89258629	MsG0480023739.01.T01:intron	40.0%
	TGACCGTGATGAAGGATAAA+GGG	-	Chr4:89255287-89255306	None:intergenic	40.0%
	TGCTCCATCACTAAAGTTTC+TGG	+	Chr4:89258558-89258577	MsG0480023739.01.T01:intron	40.0%
	TGGTGAACAAAACACTCTGT+TGG	-	Chr4:89257915-89257934	None:intergenic	40.0%
	TTCACGATTCATTTGTCGGA+GGG	+	Chr4:89261018-89261037	MsG0480023739.01.T01:three_prime_UTR	40.0%
	TTCCATCTAGCGTATTTGGA+GGG	-	Chr4:89255382-89255401	None:intergenic	40.0%
	TTGAGTGAGCAAAGTGAAAG+CGG	+	Chr4:89260697-89260716	MsG0480023739.01.T01:three_prime_UTR	40.0%
	TTGTCCTTCTGAGTGACAAT+AGG	+	Chr4:89259628-89259647	MsG0480023739.01.T01:intron	40.0%
	TTGTGGAATGCAGAGACATA+TGG	-	Chr4:89256204-89256223	None:intergenic	40.0%
	TTTATCCTTCATCACGGTCA+CGG	+	Chr4:89255287-89255306	MsG0480023739.01.T01:CDS	40.0%
!	CATTTTCCTAGCAATGTCAC+AGG	-	Chr4:89259747-89259766	None:intergenic	40.0%
!	CGAGAAGACACCTTTTATGA+AGG	+	Chr4:89256480-89256499	MsG0480023739.01.T01:intron	40.0%
!	CGGCTTTTGCAAAAATTGGA+AGG	+	Chr4:89257518-89257537	MsG0480023739.01.T01:CDS	40.0%
!	GATGATGATGATGATGAGGA+AGG	-	Chr4:89255691-89255710	None:intergenic	40.0%
!	GTAGATCTCACCGTTTTATG+TGG	-	Chr4:89259989-89260008	None:intergenic	40.0%
!	TATATTTTCCCACTCACCGA+TGG	+	Chr4:89257359-89257378	MsG0480023739.01.T01:CDS	40.0%
!!	AAATCATTGACTCGTTGCCA+AGG	-	Chr4:89255525-89255544	None:intergenic	40.0%
!!	AGCATTGTAGAAGTGTCACT+TGG	+	Chr4:89255964-89255983	MsG0480023739.01.T01:intron	40.0%
!!	GAACCGGCTTTTGCAAAAAT+TGG	+	Chr4:89257514-89257533	MsG0480023739.01.T01:CDS	40.0%
!!	TGCTTGTTTTCATCCTAGCA+CGG	+	Chr4:89259143-89259162	MsG0480023739.01.T01:intron	40.0%
!!	TGTTTGGTTGGTAGGCTAAT+TGG	-	Chr4:89259345-89259364	None:intergenic	40.0%
!!	TTCACATATTGACTCGACTG+AGG	+	Chr4:89260375-89260394	MsG0480023739.01.T01:intron	40.0%
!!	TTTTTGTTTGACAGCACTCG+AGG	+	Chr4:89257775-89257794	MsG0480023739.01.T01:intron	40.0%
!!!	GCCTCTTCTTTTTCAATCTC+AGG	+	Chr4:89255578-89255597	MsG0480023739.01.T01:CDS	40.0%
!!!	TGCTAATCGCTTTTTACGCA+AGG	+	Chr4:89259801-89259820	MsG0480023739.01.T01:intron	40.0%
	AAAGTGGAAGGGGAGCAATT+TGG	+	Chr4:89260849-89260868	MsG0480023739.01.T01:three_prime_UTR	45.0%
	AACACTGAATAGGGGAAGGA+AGG	+	Chr4:89258485-89258504	MsG0480023739.01.T01:intron	45.0%
	AACCTTCCGTCCCTAATCTA+GGG	+	Chr4:89257030-89257049	MsG0480023739.01.T01:intron	45.0%
	AACTAGACATAGGCCATCAG+GGG	-	Chr4:89260534-89260553	None:intergenic	45.0%
	AATACGCTAGATGGAACGCT+AGG	+	Chr4:89255386-89255405	MsG0480023739.01.T01:CDS	45.0%
	AATTCATGTACGCAAGGCCT+TGG	+	Chr4:89255505-89255524	MsG0480023739.01.T01:CDS	45.0%
	ACAATTAGAGAAGCTGCAGC+CGG	+	Chr4:89260440-89260459	MsG0480023739.01.T01:CDS	45.0%
	ACACTGAATAGGGGAAGGAA+GGG	+	Chr4:89258486-89258505	MsG0480023739.01.T01:intron	45.0%
	ACAGACATAGAAGAGGGGTA+AGG	+	Chr4:89256256-89256275	MsG0480023739.01.T01:intron	45.0%
	ACAGCACTCGAGGAATAGAT+GGG	+	Chr4:89257785-89257804	MsG0480023739.01.T01:intron	45.0%
	ACTCAACTGCTCAAGGACAA+AGG	+	Chr4:89256009-89256028	MsG0480023739.01.T01:CDS	45.0%
	AGCGTTCCATCTAGCGTATT+TGG	-	Chr4:89255386-89255405	None:intergenic	45.0%
	AGCTTATGGATGCTACGTGT+AGG	+	Chr4:89260486-89260505	MsG0480023739.01.T01:CDS	45.0%
	ATCACGGGCAAACATCTTGT+AGG	-	Chr4:89257555-89257574	None:intergenic	45.0%
	ATCAGTGCCTCCACAATTAG+AGG	-	Chr4:89260879-89260898	None:intergenic	45.0%
	ATCCCTCCAAATACGCTAGA+TGG	+	Chr4:89255377-89255396	MsG0480023739.01.T01:CDS	45.0%
	ATCCGAACTTCTCTGCCAAA+TGG	+	Chr4:89257324-89257343	MsG0480023739.01.T01:CDS	45.0%
	ATCTAGCGTATTTGGAGGGA+TGG	-	Chr4:89255378-89255397	None:intergenic	45.0%
	ATGCACAGACACTTGGAAGA+AGG	+	Chr4:89257059-89257078	MsG0480023739.01.T01:intron	45.0%
	ATGCTTTAACTCAGACGCCT+TGG	+	Chr4:89259670-89259689	MsG0480023739.01.T01:intron	45.0%
	CAACTAGACATAGGCCATCA+GGG	-	Chr4:89260535-89260554	None:intergenic	45.0%
	CAGACATAGAAGAGGGGTAA+GGG	+	Chr4:89256257-89256276	MsG0480023739.01.T01:intron	45.0%
	CCTGATTGTGCAAATGCCAT+GGG	+	Chr4:89260290-89260309	MsG0480023739.01.T01:intron	45.0%
	CTCCATTTGGCAGAGAAGTT+CGG	-	Chr4:89257329-89257348	None:intergenic	45.0%
	CTGTCTATACCTCTGTCAGT+TGG	-	Chr4:89256975-89256994	None:intergenic	45.0%
	GAAGGAAGGGCAATTTCTGA+AGG	+	Chr4:89258499-89258518	MsG0480023739.01.T01:intron	45.0%
	GACAAAGGTTTGCCTATCGA+AGG	+	Chr4:89256024-89256043	MsG0480023739.01.T01:CDS	45.0%
	GAGGAAACCATATTCTCCGA+AGG	+	Chr4:89259852-89259871	MsG0480023739.01.T01:intron	45.0%
	GAGGCAAAGAGAAGTTGTCA+CGG	-	Chr4:89255563-89255582	None:intergenic	45.0%
	GAGGGCTGTATCTGTATATG+AGG	+	Chr4:89257137-89257156	MsG0480023739.01.T01:CDS	45.0%
	GATGTGAACATGGGCTTGAT+TGG	-	Chr4:89261117-89261136	None:intergenic	45.0%
	GCACCAAGGAAATATGAAGG+TGG	-	Chr4:89257811-89257830	None:intergenic	45.0%
	GCTCTCTACTCTAAGCTTCT+AGG	+	Chr4:89258155-89258174	MsG0480023739.01.T01:intron	45.0%
	GCTTCGAATTCATGTACGCA+AGG	+	Chr4:89255499-89255518	MsG0480023739.01.T01:CDS	45.0%
	GGAAATCACACCTTAAGGTG+TGG	+	Chr4:89256595-89256614	MsG0480023739.01.T01:intron	45.0%
	GGACCTGTTCTCAAAATGGT+GGG	-	Chr4:89259967-89259986	None:intergenic	45.0%
	GTGAACCGAGTCCAAAATAC+AGG	-	Chr4:89260926-89260945	None:intergenic	45.0%
	GTGACCGTGATGAAGGATAA+AGG	-	Chr4:89255288-89255307	None:intergenic	45.0%
	GTGATTCTGACAGCAAGGAT+GGG	+	Chr4:89257447-89257466	MsG0480023739.01.T01:CDS	45.0%
	GTGTATCCAAAGATCGCAGT+GGG	+	Chr4:89255765-89255784	MsG0480023739.01.T01:CDS	45.0%
	GTTCCATCTAGCGTATTTGG+AGG	-	Chr4:89255383-89255402	None:intergenic	45.0%
	GTTGCTGGTCGAACAGATAA+AGG	+	Chr4:89256057-89256076	MsG0480023739.01.T01:CDS	45.0%
	TAGCAACTCTATGGTCGTAG+AGG	+	Chr4:89256685-89256704	MsG0480023739.01.T01:intron	45.0%
	TCAACTAGACATAGGCCATC+AGG	-	Chr4:89260536-89260555	None:intergenic	45.0%
	TCAGTGCCTCCACAATTAGA+GGG	-	Chr4:89260878-89260897	None:intergenic	45.0%
	TCCTGATTGTGCAAATGCCA+TGG	+	Chr4:89260289-89260308	MsG0480023739.01.T01:intron	45.0%
	TCTAGCGTATTTGGAGGGAT+GGG	-	Chr4:89255377-89255396	None:intergenic	45.0%
	TGGATTCCCTCTAATTGTGG+AGG	+	Chr4:89260869-89260888	MsG0480023739.01.T01:three_prime_UTR	45.0%
	TGTGATTCTGACAGCAAGGA+TGG	+	Chr4:89257446-89257465	MsG0480023739.01.T01:CDS	45.0%
	TGTGGGACCTGTTCTCAAAA+TGG	-	Chr4:89259971-89259990	None:intergenic	45.0%
	TGTTAGGCCCAATTGTCGTT+GGG	+	Chr4:89256334-89256353	MsG0480023739.01.T01:intron	45.0%
!	ACTGACAGAGGTATAGACAG+TGG	+	Chr4:89256975-89256994	MsG0480023739.01.T01:intron	45.0%
!	CTGACAGAGGTATAGACAGT+GGG	+	Chr4:89256976-89256995	MsG0480023739.01.T01:intron	45.0%
!	GACTCGGTTCACCCAAAATT+GGG	+	Chr4:89260934-89260953	MsG0480023739.01.T01:three_prime_UTR	45.0%
!	GATACAGCCCTCAATTTTCC+TGG	-	Chr4:89257129-89257148	None:intergenic	45.0%
!	GGCCTATGTCTAGTTGATGT+TGG	+	Chr4:89260539-89260558	MsG0480023739.01.T01:CDS	45.0%
!	GGGCAATTTCTGAAGGAGTA+AGG	+	Chr4:89258506-89258525	MsG0480023739.01.T01:intron	45.0%
!	GGTTTTGCTAGAACCCATGA+AGG	-	Chr4:89256635-89256654	None:intergenic	45.0%
!	TATCACGGCGCTTCTTTTGA+TGG	+	Chr4:89255824-89255843	MsG0480023739.01.T01:intron	45.0%
!	TCGTGGGTATTGTCGTAATC+TGG	+	Chr4:89256398-89256417	MsG0480023739.01.T01:intron	45.0%
!	TTGGTGCTTATCCTGTCCAT+CGG	-	Chr4:89257378-89257397	None:intergenic	45.0%
!!	ATCAAAAGAAGCGCCGTGAT+AGG	-	Chr4:89255825-89255844	None:intergenic	45.0%
!!	CTTGTTTTCATCCTAGCACG+GGG	+	Chr4:89259145-89259164	MsG0480023739.01.T01:intron	45.0%
!!	GCTTGTTTTCATCCTAGCAC+GGG	+	Chr4:89259144-89259163	MsG0480023739.01.T01:intron	45.0%
!!	TGTCCCACCATTTTGAGAAC+AGG	+	Chr4:89259961-89259980	MsG0480023739.01.T01:intron	45.0%
!!	TAATAAATAAAAAAATATAC+TGG	-	Chr4:89259595-89259614	None:intergenic	5.0%
	AACCCTAGATTAGGGACGGA+AGG	-	Chr4:89257035-89257054	None:intergenic	50.0%
	ACAGCAGCACAACCTTCGAT+AGG	-	Chr4:89256039-89256058	None:intergenic	50.0%
	ACATAGAAGAGGGGTAAGGG+CGG	+	Chr4:89256260-89256279	MsG0480023739.01.T01:intron	50.0%
	ACGCAGCACCAGGAAAATTG+AGG	+	Chr4:89257118-89257137	MsG0480023739.01.T01:CDS	50.0%
	ACTAGACATAGGCCATCAGG+GGG	-	Chr4:89260533-89260552	None:intergenic	50.0%
	AGGCTGCGACTAATGTCAAG+TGG	-	Chr4:89259000-89259019	None:intergenic	50.0%
	ATGATGGTTTCTTGGACCGG+TGG	+	Chr4:89258781-89258800	MsG0480023739.01.T01:intron	50.0%
	CAAAGATCGCAGTGGGAAGT+GGG	+	Chr4:89255772-89255791	MsG0480023739.01.T01:intron	50.0%
	CAGAATCCATAGGCCAGTAG+TGG	-	Chr4:89258255-89258274	None:intergenic	50.0%
	CAGGATAAGCACCAAAGTGG+CGG	+	Chr4:89257383-89257402	MsG0480023739.01.T01:CDS	50.0%
	CATTGCTCTTAAACTCGCCG+TGG	+	Chr4:89260816-89260835	MsG0480023739.01.T01:three_prime_UTR	50.0%
	CCCATGGCATTTGCACAATC+AGG	-	Chr4:89260293-89260312	None:intergenic	50.0%
	CGAACTTCTCTGCCAAATGG+AGG	+	Chr4:89257327-89257346	MsG0480023739.01.T01:CDS	50.0%
	CGCAGCACCAGGAAAATTGA+GGG	+	Chr4:89257119-89257138	MsG0480023739.01.T01:CDS	50.0%
	CGGCGAGTTTAAGAGCAATG+TGG	-	Chr4:89260816-89260835	None:intergenic	50.0%
	CGTATTTGGAGGGATGGGTA+TGG	-	Chr4:89255372-89255391	None:intergenic	50.0%
	GAACCTTCCGTCCCTAATCT+AGG	+	Chr4:89257029-89257048	MsG0480023739.01.T01:intron	50.0%
	GACAGCACTCGAGGAATAGA+TGG	+	Chr4:89257784-89257803	MsG0480023739.01.T01:intron	50.0%
	GCAATAAATGACGCAGCACC+AGG	+	Chr4:89257108-89257127	MsG0480023739.01.T01:CDS	50.0%
	GGACAGGATAAGCACCAAAG+TGG	+	Chr4:89257380-89257399	MsG0480023739.01.T01:CDS	50.0%
	GGCAGAGAAGTTCGGATGAA+AGG	-	Chr4:89257321-89257340	None:intergenic	50.0%
	GGCTGCAGCTTCTCTAATTG+TGG	-	Chr4:89260441-89260460	None:intergenic	50.0%
	GGGACCTGTTCTCAAAATGG+TGG	-	Chr4:89259968-89259987	None:intergenic	50.0%
	GGGCCACCTTCATATTTCCT+TGG	+	Chr4:89257805-89257824	MsG0480023739.01.T01:intron	50.0%
	GGTGTATCCAAAGATCGCAG+TGG	+	Chr4:89255764-89255783	MsG0480023739.01.T01:CDS	50.0%
	GTGTTAGGCCCAATTGTCGT+TGG	+	Chr4:89256333-89256352	MsG0480023739.01.T01:intron	50.0%
	GTTAGGCCCAATTGTCGTTG+GGG	+	Chr4:89256335-89256354	MsG0480023739.01.T01:intron	50.0%
	TATCCTGTCCATCGGTGAGT+GGG	-	Chr4:89257370-89257389	None:intergenic	50.0%
	TCAGCGTAAGGAAGGTGAAC+CGG	+	Chr4:89257498-89257517	MsG0480023739.01.T01:CDS	50.0%
	TGAAAGCGGATGCATGCACA+AGG	+	Chr4:89260711-89260730	MsG0480023739.01.T01:three_prime_UTR	50.0%
	TGTTCACCATGCTCGAGCTA+TGG	+	Chr4:89257926-89257945	MsG0480023739.01.T01:CDS	50.0%
	TTAGGCCCAATTGTCGTTGG+GGG	+	Chr4:89256336-89256355	MsG0480023739.01.T01:intron	50.0%
	TTATCCTGTCCATCGGTGAG+TGG	-	Chr4:89257371-89257390	None:intergenic	50.0%
!	CAAGGCATCATCTTCAGCAC+CGG	-	Chr4:89260462-89260481	None:intergenic	50.0%
!	GCAGATGGTTAGAGTGCTTG+TGG	+	Chr4:89260412-89260431	MsG0480023739.01.T01:intron	50.0%
!	GGACTCGGTTCACCCAAAAT+TGG	+	Chr4:89260933-89260952	MsG0480023739.01.T01:three_prime_UTR	50.0%
!!	TGGATGTGACTTGCTCCAAC+TGG	+	Chr4:89258114-89258133	MsG0480023739.01.T01:intron	50.0%
	AAGCACCAAAGTGGCGGCAA+TGG	+	Chr4:89257389-89257408	MsG0480023739.01.T01:CDS	55.0%
	AATTGTCGTTGGGGGAGACG+GGG	+	Chr4:89256344-89256363	MsG0480023739.01.T01:intron	55.0%
	AGCACCAAAGTGGCGGCAAT+GGG	+	Chr4:89257390-89257409	MsG0480023739.01.T01:CDS	55.0%
	ATACCCACGAACCATGACCG+CGG	-	Chr4:89256388-89256407	None:intergenic	55.0%
	CAATTGTCGTTGGGGGAGAC+GGG	+	Chr4:89256343-89256362	MsG0480023739.01.T01:intron	55.0%
	CCAAAGATCGCAGTGGGAAG+TGG	+	Chr4:89255771-89255790	MsG0480023739.01.T01:intron	55.0%
	CCAATTGTCGTTGGGGGAGA+CGG	+	Chr4:89256342-89256361	MsG0480023739.01.T01:intron	55.0%
	CCACTTCCCACTGCGATCTT+TGG	-	Chr4:89255774-89255793	None:intergenic	55.0%
	CGTCTCCCCCAACGACAATT+GGG	-	Chr4:89256344-89256363	None:intergenic	55.0%
	CTTAAACTCGCCGTGGTGCA+GGG	+	Chr4:89260823-89260842	MsG0480023739.01.T01:three_prime_UTR	55.0%
	GTGCAGGGGAGAAAGTGGAA+GGG	+	Chr4:89260838-89260857	MsG0480023739.01.T01:three_prime_UTR	55.0%
	TAGCTGAAGAACCCCGTGCT+AGG	-	Chr4:89259159-89259178	None:intergenic	55.0%
	TCCAGAGACATGGCCACTAC+TGG	+	Chr4:89258239-89258258	MsG0480023739.01.T01:intron	55.0%
	TCTTAAACTCGCCGTGGTGC+AGG	+	Chr4:89260822-89260841	MsG0480023739.01.T01:three_prime_UTR	55.0%
	TGCAGGGGAGAAAGTGGAAG+GGG	+	Chr4:89260839-89260858	MsG0480023739.01.T01:three_prime_UTR	55.0%
	TGGCTTCCATAGCTCGAGCA+TGG	-	Chr4:89257935-89257954	None:intergenic	55.0%
	TGGGCCTAACACACGCGTTT+GGG	-	Chr4:89256325-89256344	None:intergenic	55.0%
	TTAAACTCGCCGTGGTGCAG+GGG	+	Chr4:89260824-89260843	MsG0480023739.01.T01:three_prime_UTR	55.0%
	TTGGCCCAAACGCGTGTGTT+AGG	+	Chr4:89256318-89256337	MsG0480023739.01.T01:intron	55.0%
	TTGGGCCTAACACACGCGTT+TGG	-	Chr4:89256326-89256345	None:intergenic	55.0%
	TTTCCCACTCACCGATGGAC+AGG	+	Chr4:89257364-89257383	MsG0480023739.01.T01:CDS	55.0%
!	ACATGGCCACTACTGGCCTA+TGG	+	Chr4:89258246-89258265	MsG0480023739.01.T01:intron	55.0%
!	GAAGGTTGTGCTGCTGTTGC+TGG	+	Chr4:89256042-89256061	MsG0480023739.01.T01:CDS	55.0%
!!	ACGGCGCTTCTTTTGATGGC+TGG	+	Chr4:89255828-89255847	MsG0480023739.01.T01:intron	55.0%
	AATGCCATGGGCGTGCAGTC+TGG	+	Chr4:89260302-89260321	MsG0480023739.01.T01:intron	60.0%
	ACGCGTTTGGGCCAAACCCA+AGG	-	Chr4:89256313-89256332	None:intergenic	60.0%
	ACTCCCCATTGCCGCCACTT+TGG	-	Chr4:89257397-89257416	None:intergenic	60.0%
	AGCGTGCACACTCTGCCAGT+TGG	-	Chr4:89258132-89258151	None:intergenic	60.0%
	ATTGTCGTTGGGGGAGACGG+GGG	+	Chr4:89256345-89256364	MsG0480023739.01.T01:intron	60.0%
	CAACGCCGTGACCGTGATGA+AGG	-	Chr4:89255295-89255314	None:intergenic	60.0%
	CCACTTTCTCCCCTGCACCA+CGG	-	Chr4:89260836-89260855	None:intergenic	60.0%
	CCGTCTCCCCCAACGACAAT+TGG	-	Chr4:89256345-89256364	None:intergenic	60.0%
	CGCGTTTGGGCCAAACCCAA+GGG	-	Chr4:89256312-89256331	None:intergenic	60.0%
	GATGACCCAGCTCAAGCCAC+CGG	-	Chr4:89258800-89258819	None:intergenic	60.0%
	GCACCAAAGTGGCGGCAATG+GGG	+	Chr4:89257391-89257410	MsG0480023739.01.T01:CDS	60.0%
	GCCAGTAGTGGCCATGTCTC+TGG	-	Chr4:89258243-89258262	None:intergenic	60.0%
	GGTGCAGGGGAGAAAGTGGA+AGG	+	Chr4:89260837-89260856	MsG0480023739.01.T01:three_prime_UTR	60.0%
	TGTACCAGACTGCACGCCCA+TGG	-	Chr4:89260309-89260328	None:intergenic	60.0%
	TTGGACCGGTGGCTTGAGCT+GGG	+	Chr4:89258792-89258811	MsG0480023739.01.T01:intron	60.0%
	TTGGGCCAAACCCAAGGGCA+GGG	-	Chr4:89256307-89256326	None:intergenic	60.0%
	TTTGGGCCAAACCCAAGGGC+AGG	-	Chr4:89256308-89256327	None:intergenic	60.0%
!	GAACCATGACCGCGGTGCTA+TGG	-	Chr4:89256380-89256399	None:intergenic	60.0%
	AAACCCAAGGGCAGGGGGGA+GGG	-	Chr4:89256300-89256319	None:intergenic	65.0%
	AGCACCGCGGTCATGGTTCG+TGG	+	Chr4:89256381-89256400	MsG0480023739.01.T01:intron	65.0%
	CCGTGGTGCAGGGGAGAAAG+TGG	+	Chr4:89260833-89260852	MsG0480023739.01.T01:three_prime_UTR	65.0%
	CTTGGACCGGTGGCTTGAGC+TGG	+	Chr4:89258791-89258810	MsG0480023739.01.T01:intron	65.0%
	GCACCGCGGTCATGGTTCGT+GGG	+	Chr4:89256382-89256401	MsG0480023739.01.T01:intron	65.0%
	GGACCATAGCACCGCGGTCA+TGG	+	Chr4:89256374-89256393	MsG0480023739.01.T01:intron	65.0%
	TGGGCCAAACCCAAGGGCAG+GGG	-	Chr4:89256306-89256325	None:intergenic	65.0%
!	CATAGGCCATCAGGGGGTGC+TGG	-	Chr4:89260527-89260546	None:intergenic	65.0%
	CAAACCCAAGGGCAGGGGGG+AGG	-	Chr4:89256301-89256320	None:intergenic	70.0%
	CTCCCCCCTGCCCTTGGGTT+TGG	+	Chr4:89256299-89256318	MsG0480023739.01.T01:intron	70.0%
	GCTCCTCCAGCACCCCCTGA+TGG	+	Chr4:89260518-89260537	MsG0480023739.01.T01:CDS	70.0%
	GGCCAAACCCAAGGGCAGGG+GGG	-	Chr4:89256304-89256323	None:intergenic	70.0%
	GGGCCAAACCCAAGGGCAGG+GGG	-	Chr4:89256305-89256324	None:intergenic	70.0%
	TGCGGGGGACCATAGCACCG+CGG	+	Chr4:89256368-89256387	MsG0480023739.01.T01:intron	70.0%
!!	AGGCCATCAGGGGGTGCTGG+AGG	-	Chr4:89260524-89260543	None:intergenic	70.0%
!!	AGGGGGTGCTGGAGGAGCAG+TGG	-	Chr4:89260516-89260535	None:intergenic	70.0%
	AGCTCCCTCCCCCCTGCCCT+TGG	+	Chr4:89256293-89256312	MsG0480023739.01.T01:intron	75.0%
	CGTTGGGGGAGACGGGGGTG+CGG	+	Chr4:89256350-89256369	MsG0480023739.01.T01:intron	75.0%
	GCTCCCTCCCCCCTGCCCTT+GGG	+	Chr4:89256294-89256313	MsG0480023739.01.T01:intron	75.0%
!	GTTGGGGGAGACGGGGGTGC+GGG	+	Chr4:89256351-89256370	MsG0480023739.01.T01:intron	75.0%
!	TTGGGGGAGACGGGGGTGCG+GGG	+	Chr4:89256352-89256371	MsG0480023739.01.T01:intron	75.0%
!!	TGGGGGAGACGGGGGTGCGG+GGG	+	Chr4:89256353-89256372	MsG0480023739.01.T01:intron	80.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	89255209	89261136	89255209	ID=MsG0480023739.01;Name=MsG0480023739.01
Chr4	mRNA	89255209	89261136	89255209	ID=MsG0480023739.01.T01;Parent=MsG0480023739.01;Name=MsG0480023739.01.T01;_AED=0.47;_eAED=0.47;_QI=8\|0.71\|0.87\|1\|1\|1\|8\|529\|393
Chr4	exon	89255209	89255407	89255209	ID=MsG0480023739.01.T01:exon:13038;Parent=MsG0480023739.01.T01
Chr4	exon	89255494	89255599	89255494	ID=MsG0480023739.01.T01:exon:13039;Parent=MsG0480023739.01.T01
Chr4	exon	89255686	89255789	89255686	ID=MsG0480023739.01.T01:exon:13040;Parent=MsG0480023739.01.T01
Chr4	exon	89255966	89256111	89255966	ID=MsG0480023739.01.T01:exon:13041;Parent=MsG0480023739.01.T01
Chr4	exon	89257067	89257158	89257067	ID=MsG0480023739.01.T01:exon:13042;Parent=MsG0480023739.01.T01
Chr4	exon	89257308	89257594	89257308	ID=MsG0480023739.01.T01:exon:13043;Parent=MsG0480023739.01.T01
Chr4	exon	89257905	89257968	89257905	ID=MsG0480023739.01.T01:exon:13044;Parent=MsG0480023739.01.T01
Chr4	exon	89260416	89261136	89260416	ID=MsG0480023739.01.T01:exon:13045;Parent=MsG0480023739.01.T01
Chr4	five_prime_UTR	89255209	89255216	89255209	ID=MsG0480023739.01.T01:five_prime_utr;Parent=MsG0480023739.01.T01
Chr4	CDS	89255217	89255407	89255217	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89255494	89255599	89255494	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89255686	89255789	89255686	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89255966	89256111	89255966	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89257067	89257158	89257067	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89257308	89257594	89257308	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89257905	89257968	89257905	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	CDS	89260416	89260607	89260416	ID=MsG0480023739.01.T01:cds;Parent=MsG0480023739.01.T01
Chr4	three_prime_UTR	89260608	89261136	89260608	ID=MsG0480023739.01.T01:three_prime_utr;Parent=MsG0480023739.01.T01

Gene Sequence

>MsG0480023739.01.T01

ATGCATTATTCTTCTATTAGGAAGCACATGTTGATGGGTTCCTTCTTGTTCAGACACAACAACATTTCCCTTTATCCTTCATCACGGTCACGGCGTTGTAATTATTCGATGGAACACCAAAGTGAAAGAGATAGTCATCATGTTCATTACAACCATACCCATCCCTCCAAATACGCTAGATGGAACGCTAGGGAGAGCTTCGAATTCATGTACGCAAGGCCTTGGCAACGAGTCAATGATTTTTACTTGAACACTGTCCGTGACAACTTCTCTTTGCCTCTTCTTTTTCAATCTCAGACCCTTCCTCATCATCATCATCATCATCATCATTCTGGAGTTCCAGAAATTTTGGATAATACTGAGTTCGAAAGTGATGGTGTATCCAAAGATCGCAGTGGGAACATTGTAGAAGTGTCACTTGGTAAATTTGTTGATGAAAACAAAACTCAACTGCTCAAGGACAAAGGTTTGCCTATCGAAGGTTGTGCTGCTGTTGCTGGTCGAACAGATAAAGGAGTCACAGCCCTTCAACAAGTTTGTTCTTTTTACACTTGGAAGAAGGATATTAAACCAAAAGAAATTGAAGATGCAATAAATGACGCAGCACCAGGAAAATTGAGGGCTGTATCTGTATATGAGGTATCACGTGCCTTTCATCCGAACTTCTCTGCCAAATGGAGGCGTTACCTTTATATTTTCCCACTCACCGATGGACAGGATAAGCACCAAAGTGGCGGCAATGGGGAGTCTTGTGACAGTTTTATATATAATGGAATTTGTGATTCTGACAGCAAGGATGGGTTAGAAAATGAAAATAAATCATATGTGTTCAGCGTAAGGAAGGTGAACCGGCTTTTGCAAAAATTGGAAGGAAAATTGCTGTCCTACAAGATGTTTGCCCGTGATACAAAAGCTTCAAGAAACGATGGTCCACCAACAGAGTGTTTTGTTCACCATGCTCGAGCTATGGAAGCCAAATTGCCTATAACTATGGTTAGAGTGCTTGTGGCAACCACAATTAGAGAAGCTGCAGCCGGTGCTGAAGATGATGCCTTGTTAAAGCTTATGGATGCTACGTGTAGGCGTGCCACTGCTCCTCCAGCACCCCCTGATGGCCTATGTCTAGTTGATGTTGGTTATGCAGAATTTGAACGGGAAAAATGTTTCATCATGAAAGATTAG

Protein sequence

>MsG0480023739.01.T01

MHYSSIRKHMLMGSFLFRHNNISLYPSSRSRRCNYSMEHQSERDSHHVHYNHTHPSKYARWNARESFEFMYARPWQRVNDFYLNTVRDNFSLPLLFQSQTLPHHHHHHHHSGVPEILDNTEFESDGVSKDRSGNIVEVSLGKFVDENKTQLLKDKGLPIEGCAAVAGRTDKGVTALQQVCSFYTWKKDIKPKEIEDAINDAAPGKLRAVSVYEVSRAFHPNFSAKWRRYLYIFPLTDGQDKHQSGGNGESCDSFIYNGICDSDSKDGLENENKSYVFSVRKVNRLLQKLEGKLLSYKMFARDTKASRNDGPPTECFVHHARAMEAKLPITMVRVLVATTIREAAAGAEDDALLKLMDATCRRATAPPAPPDGLCLVDVGYAEFEREKCFIMKD*