Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045017.01.T01 | XP_003628687.1 | 100 | 116 | 0 | 0 | 1 | 116 | 1 | 116 | 2.94E-75 | 228 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047791.01 | MsG0880045017.01 | 0.818162 | 2.263849e-52 | 1.681682e-49 |
| MsG0080048065.01 | MsG0880045017.01 | 0.808426 | 3.115062e-50 | 1.780607e-47 |
| MsG0080048683.01 | MsG0880045017.01 | 0.801484 | 8.811836e-49 | 4.213101e-46 |
| MsG0080048878.01 | MsG0880045017.01 | 0.803176 | 3.949336e-49 | 1.971355e-46 |
| MsG0180000114.01 | MsG0880045017.01 | 0.804994 | 1.653853e-49 | 8.650472e-47 |
| MsG0180000384.01 | MsG0880045017.01 | 0.800365 | 1.491483e-48 | 6.932042e-46 |
| MsG0180000568.01 | MsG0880045017.01 | 0.820234 | 7.637532e-53 | 6.009065e-50 |
| MsG0180000611.01 | MsG0880045017.01 | 0.808666 | 2.768379e-50 | 1.592305e-47 |
| MsG0180000777.01 | MsG0880045017.01 | 0.828646 | 8.006260e-55 | 8.006277e-52 |
| MsG0180000806.01 | MsG0880045017.01 | 0.811564 | 6.574693e-51 | 4.083292e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045017.01.T01 | MTR_8g063460 | 100.000 | 116 | 0 | 0 | 1 | 116 | 1 | 116 | 3.56e-79 | 228 |
Find 35 sgRNAs with CRISPR-Local
Find 290 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGAAAAGTGTCTTAAT+GGG | 0.346020 | 8:-52783486 | MsG0880045017.01.T01:CDS |
| AAAGAGGAATGTTCCTGCTG+AGG | 0.625640 | 8:-52783314 | MsG0880045017.01.T01:three_prime_UTR |
| AACTCAACTAATGTACCTCT+AGG | 0.611731 | 8:-52783273 | MsG0880045017.01.T01:three_prime_UTR |
| AAGAGAAAAGTGTCTTAATG+GGG | 0.648948 | 8:-52783485 | MsG0880045017.01.T01:CDS |
| ACTTTGCTGGGATGACAAAA+AGG | 0.366427 | 8:-52783368 | MsG0880045017.01.T01:three_prime_UTR |
| AGAGATAATAATCAAAGCAA+AGG | 0.581327 | 8:-52787419 | MsG0880045017.01.T01:intron |
| AGAGGTACATTAGTTGAGTT+AGG | 0.509450 | 8:+52783276 | None:intergenic |
| AGCAGGAACATTCCTCTTTG+GGG | 0.594886 | 8:+52783318 | None:intergenic |
| AGCATTGGATGCTGATATTC+AGG | 0.319410 | 8:-52786904 | MsG0880045017.01.T01:CDS |
| ATTTCGATTCATGTTCCTAG+AGG | 0.606804 | 8:+52783258 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGAATTCCATTGTAGA+AGG | - | Chr8:52784863-52784882 | MsG0880045017.01.T01:intron | 30.0% | |
| AACAAAATGCCCTTGAAATT+TGG | - | Chr8:52786567-52786586 | MsG0880045017.01.T01:intron | 30.0% | |
| AACAATCAATAGCAAAGCTA+AGG | + | Chr8:52784736-52784755 | None:intergenic | 30.0% | |
| AAGAAAAAGTAAGACTTTGC+TGG | - | Chr8:52787214-52787233 | MsG0880045017.01.T01:intron | 30.0% | |
| AAGAGAATTCCATTGTAGAA+GGG | - | Chr8:52784864-52784883 | MsG0880045017.01.T01:intron | 30.0% | |
| AATTGTATAATGGTGTCTGT+TGG | - | Chr8:52785859-52785878 | MsG0880045017.01.T01:intron | 30.0% | |
| ACAAATTGTCCTATAACCTT+AGG | + | Chr8:52785044-52785063 | None:intergenic | 30.0% | |
| ACAATCAATAGCAAAGCTAA+GGG | + | Chr8:52784735-52784754 | None:intergenic | 30.0% | |
| ACCATGTCCAATATATACAA+CGG | + | Chr8:52785987-52786006 | None:intergenic | 30.0% | |
| ACTGGTAACATCCAAAATAT+AGG | + | Chr8:52786030-52786049 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 52783170 | 52787447 | 52783170 | ID=MsG0880045017.01;Name=MsG0880045017.01 |
| Chr8 | mRNA | 52783170 | 52787447 | 52783170 | ID=MsG0880045017.01.T01;Parent=MsG0880045017.01;Name=MsG0880045017.01.T01;_AED=0.44;_eAED=0.44;_QI=47|1|1|1|0.5|0.33|3|222|116 |
| Chr8 | exon | 52783170 | 52783562 | 52783170 | ID=MsG0880045017.01.T01:exon:22676;Parent=MsG0880045017.01.T01 |
| Chr8 | exon | 52786800 | 52786998 | 52786800 | ID=MsG0880045017.01.T01:exon:22675;Parent=MsG0880045017.01.T01 |
| Chr8 | exon | 52787420 | 52787447 | 52787420 | ID=MsG0880045017.01.T01:exon:22674;Parent=MsG0880045017.01.T01 |
| Chr8 | five_prime_UTR | 52787420 | 52787447 | 52787420 | ID=MsG0880045017.01.T01:five_prime_utr;Parent=MsG0880045017.01.T01 |
| Chr8 | five_prime_UTR | 52786980 | 52786998 | 52786980 | ID=MsG0880045017.01.T01:five_prime_utr;Parent=MsG0880045017.01.T01 |
| Chr8 | CDS | 52786800 | 52786979 | 52786800 | ID=MsG0880045017.01.T01:cds;Parent=MsG0880045017.01.T01 |
| Chr8 | CDS | 52783392 | 52783562 | 52783392 | ID=MsG0880045017.01.T01:cds;Parent=MsG0880045017.01.T01 |
| Chr8 | three_prime_UTR | 52783170 | 52783391 | 52783170 | ID=MsG0880045017.01.T01:three_prime_utr;Parent=MsG0880045017.01.T01 |
| Gene Sequence |
>MsG0880045017.01.T01 ATGTCTCTGCGGATAAAAGCTGTGGTGGATAAATTCGTTGAGGAGTTGAAGGAAGCATTGGATGCTGATATTCAGGATAGGATCATGAAGGACAGAGAGATGCAAAGTTACATCCAAGAACGTGAGCGCGAGGTTGCTGAGCGTGAAGCTGCTTGGAAGGCCGATCTTTCTCGTCGTGAGGCAGAGATTGTTCGTCAAGAAGCAAGATTGAAGATGGAGAGAGATAATCTTGAGAAAGAGAAAAGTGTCTTAATGGGGACTGCATCAAATCAAGATAACCAAGATGGAGCTCTTGAAATTACAGTTAGTGGTGAAAAATACCGTTGCCTCAGATTTCAAAAGAAAAAGTAA |
| Protein sequence |
>MsG0880045017.01.T01 MSLRIKAVVDKFVEELKEALDADIQDRIMKDREMQSYIQEREREVAEREAAWKADLSRREAEIVRQEARLKMERDNLEKEKSVLMGTASNQDNQDGALEITVSGEKYRCLRFQKKK* |
