Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025527.01.T01 | XP_003612908.1 | 71.429 | 238 | 54 | 5 | 1 | 227 | 1 | 235 | 4.66E-108 | 321 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025527.01.T01 | A0A3Q7HRZ6 | 61.458 | 192 | 71 | 3 | 23 | 212 | 89 | 279 | 4.78E-74 | 240 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025527.01.T01 | G7KF31 | 71.429 | 238 | 54 | 5 | 1 | 227 | 1 | 235 | 2.23e-108 | 321 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048036.01 | MsG0580025527.01 | 0.824079 | 9.802846e-54 | 8.594523e-51 |
| MsG0080048347.01 | MsG0580025527.01 | 0.829218 | 5.821622e-55 | 5.920409e-52 |
| MsG0080048352.01 | MsG0580025527.01 | 0.834400 | 3.063666e-56 | 3.631648e-53 |
| MsG0080048889.01 | MsG0580025527.01 | 0.800258 | 1.567776e-48 | 7.266786e-46 |
| MsG0080049038.01 | MsG0580025527.01 | -0.812897 | 3.364349e-51 | 2.165096e-48 |
| MsG0080049066.01 | MsG0580025527.01 | -0.816739 | 4.735637e-52 | 3.383257e-49 |
| MsG0080049133.01 | MsG0580025527.01 | -0.802482 | 5.492988e-49 | 2.693756e-46 |
| MsG0180000929.01 | MsG0580025527.01 | 0.812990 | 3.209659e-51 | 2.070696e-48 |
| MsG0180002935.01 | MsG0580025527.01 | 0.800383 | 1.479040e-48 | 6.877392e-46 |
| MsG0180003757.01 | MsG0580025527.01 | -0.814117 | 1.814181e-51 | 1.206718e-48 |
| MsG0180004203.01 | MsG0580025527.01 | 0.819694 | 1.015472e-52 | 7.870183e-50 |
| MsG0180004328.01 | MsG0580025527.01 | 0.808468 | 3.051742e-50 | 1.746317e-47 |
| MsG0180004502.01 | MsG0580025527.01 | -0.814855 | 1.245387e-51 | 8.450817e-49 |
| MsG0180005356.01 | MsG0580025527.01 | 0.815534 | 8.799615e-52 | 6.083059e-49 |
| MsG0180005357.01 | MsG0580025527.01 | 0.816200 | 6.251576e-52 | 4.401174e-49 |
| MsG0180005358.01 | MsG0580025527.01 | 0.821731 | 3.455120e-53 | 2.834148e-50 |
| MsG0180006212.01 | MsG0580025527.01 | -0.805370 | 1.379391e-49 | 7.284953e-47 |
| MsG0280006381.01 | MsG0580025527.01 | -0.808517 | 2.979216e-50 | 1.706971e-47 |
| MsG0280010802.01 | MsG0580025527.01 | 0.825717 | 4.025122e-54 | 3.698152e-51 |
| MsG0280011165.01 | MsG0580025527.01 | 0.836926 | 7.028299e-57 | 8.987986e-54 |
| MsG0280011193.01 | MsG0580025527.01 | 0.827827 | 1.261954e-54 | 1.232095e-51 |
| MsG0280011203.01 | MsG0580025527.01 | 0.822032 | 2.943212e-53 | 2.434930e-50 |
| MsG0280011301.01 | MsG0580025527.01 | -0.803107 | 4.082568e-49 | 2.034119e-46 |
| MsG0380011635.01 | MsG0580025527.01 | 0.816737 | 4.740127e-52 | 3.386287e-49 |
| MsG0380011974.01 | MsG0580025527.01 | 0.800257 | 1.569041e-48 | 7.272384e-46 |
| MsG0380013114.01 | MsG0580025527.01 | -0.802161 | 6.395936e-49 | 3.111065e-46 |
| MsG0380013272.01 | MsG0580025527.01 | 0.832739 | 7.960713e-56 | 8.977851e-53 |
| MsG0380014642.01 | MsG0580025527.01 | -0.816942 | 4.262842e-52 | 3.062833e-49 |
| MsG0380014735.01 | MsG0580025527.01 | -0.814856 | 1.245224e-51 | 8.449786e-49 |
| MsG0480018628.01 | MsG0580025527.01 | -0.800103 | 1.685899e-48 | 7.783557e-46 |
| MsG0480021131.01 | MsG0580025527.01 | 0.829199 | 5.882501e-55 | 5.979271e-52 |
| MsG0480021327.01 | MsG0580025527.01 | -0.802064 | 6.697382e-49 | 3.249603e-46 |
| MsG0480022655.01 | MsG0580025527.01 | 0.806558 | 7.760149e-50 | 4.224993e-47 |
| MsG0480023067.01 | MsG0580025527.01 | 0.804704 | 1.901149e-49 | 9.870025e-47 |
| MsG0480023107.01 | MsG0580025527.01 | 0.809345 | 1.981180e-50 | 1.160371e-47 |
| MsG0480023152.01 | MsG0580025527.01 | -0.813568 | 2.397102e-51 | 1.570922e-48 |
| MsG0580024408.01 | MsG0580025527.01 | 0.830465 | 2.892265e-55 | 3.049344e-52 |
| MsG0580024493.01 | MsG0580025527.01 | -0.808810 | 2.579280e-50 | 1.489135e-47 |
| MsG0580025105.01 | MsG0580025527.01 | -0.808143 | 3.579259e-50 | 2.030813e-47 |
| MsG0580025527.01 | MsG0580026492.01 | 0.803178 | 3.945968e-49 | 1.969758e-46 |
| MsG0580025527.01 | MsG0580027337.01 | 0.800265 | 1.563072e-48 | 7.246283e-46 |
| MsG0580025527.01 | MsG0580027638.01 | 0.807373 | 5.218000e-50 | 2.901377e-47 |
| MsG0580025527.01 | MsG0580027646.01 | 0.810559 | 1.085246e-50 | 6.562791e-48 |
| MsG0580025527.01 | MsG0580029202.01 | 0.814659 | 1.376359e-51 | 9.290322e-49 |
| MsG0580025527.01 | MsG0580029296.01 | 0.800119 | 1.673720e-48 | 7.730295e-46 |
| MsG0580025527.01 | MsG0580030133.01 | 0.802912 | 4.479410e-49 | 2.220936e-46 |
| MsG0580025527.01 | MsG0680031813.01 | -0.801317 | 9.533822e-49 | 4.539136e-46 |
| MsG0580025527.01 | MsG0680034229.01 | -0.803532 | 3.332257e-49 | 1.678690e-46 |
| MsG0580025527.01 | MsG0680035584.01 | -0.811663 | 6.256670e-51 | 3.895699e-48 |
| MsG0580025527.01 | MsG0680035841.01 | 0.813782 | 2.150013e-51 | 1.417192e-48 |
| MsG0580025527.01 | MsG0780036661.01 | 0.816677 | 4.888797e-52 | 3.486735e-49 |
| MsG0580025527.01 | MsG0780038722.01 | 0.803374 | 3.593374e-49 | 1.802737e-46 |
| MsG0580025527.01 | MsG0780038922.01 | 0.811841 | 5.722937e-51 | 3.580390e-48 |
| MsG0580025527.01 | MsG0780039431.01 | 0.809218 | 2.108902e-50 | 1.231004e-47 |
| MsG0580025527.01 | MsG0780039836.01 | -0.802881 | 4.546254e-49 | 2.252244e-46 |
| MsG0580025527.01 | MsG0780039839.01 | -0.813569 | 2.395559e-51 | 1.569957e-48 |
| MsG0580025527.01 | MsG0780039883.01 | 0.801142 | 1.035404e-48 | 4.908019e-46 |
| MsG0580025527.01 | MsG0780039968.01 | 0.839364 | 1.656190e-57 | 2.282015e-54 |
| MsG0580025527.01 | MsG0780039978.01 | 0.807923 | 3.987973e-50 | 2.249386e-47 |
| MsG0580025527.01 | MsG0780040121.01 | -0.803797 | 2.936768e-49 | 1.489481e-46 |
| MsG0580025527.01 | MsG0780040805.01 | 0.813257 | 2.804343e-51 | 1.822284e-48 |
| MsG0580025527.01 | MsG0780040806.01 | 0.834065 | 3.716565e-56 | 4.361375e-53 |
| MsG0580025527.01 | MsG0780041198.01 | 0.828653 | 7.977134e-55 | 7.978830e-52 |
| MsG0580025527.01 | MsG0780041316.01 | 0.805638 | 1.211779e-49 | 6.444174e-47 |
| MsG0580025527.01 | MsG0780041556.01 | 0.814626 | 1.399625e-51 | 9.439296e-49 |
| MsG0580025527.01 | MsG0780041620.01 | -0.807961 | 3.912832e-50 | 2.209332e-47 |
| MsG0580025527.01 | MsG0780041720.01 | -0.814987 | 1.164079e-51 | 7.927541e-49 |
| MsG0580025527.01 | MsG0780041783.01 | -0.824008 | 1.018690e-53 | 8.912844e-51 |
| MsG0580025527.01 | MsG0880041855.01 | -0.801305 | 9.585380e-49 | 4.562331e-46 |
| MsG0580025527.01 | MsG0880042740.01 | 0.808554 | 2.926143e-50 | 1.678150e-47 |
| MsG0580025527.01 | MsG0880042858.01 | -0.810007 | 1.427809e-50 | 8.510277e-48 |
| MsG0580025527.01 | MsG0880043364.01 | 0.805796 | 1.122872e-49 | 5.995610e-47 |
| MsG0580025527.01 | MsG0880044339.01 | -0.803974 | 2.698708e-49 | 1.375006e-46 |
| MsG0580025527.01 | MsG0880045369.01 | -0.812640 | 3.829289e-51 | 2.447757e-48 |
| MsG0580025527.01 | MsG0880045385.01 | -0.815703 | 8.072030e-52 | 5.605872e-49 |
| MsG0580025527.01 | MsG0880045458.01 | 0.816334 | 5.834354e-52 | 4.122644e-49 |
| MsG0580025527.01 | MsG0880046308.01 | 0.812119 | 4.975882e-51 | 3.136609e-48 |
| MsG0580025527.01 | MsG0880046341.01 | -0.806841 | 6.762018e-50 | 3.708504e-47 |
| MsG0580025527.01 | MsG0880046429.01 | 0.816308 | 5.911655e-52 | 4.174359e-49 |
| MsG0580025527.01 | MsG0880046469.01 | -0.820044 | 8.442189e-53 | 6.607961e-50 |
| MsG0580025527.01 | MsG0880046996.01 | 0.835225 | 1.898914e-56 | 2.307510e-53 |
| MsG0580025527.01 | MsG0880047318.01 | -0.806255 | 8.988044e-50 | 4.855962e-47 |
| MsG0580025527.01 | MsG0880047493.01 | 0.831813 | 1.349519e-55 | 1.480998e-52 |
| MsG0580025527.01 | MsG0880047714.01 | 0.817312 | 3.520271e-52 | 2.554715e-49 |
| MsG0380015080.01 | MsG0580025527.01 | 0.814863 | 1.240169e-51 | 8.417128e-49 |
| MsG0380015323.01 | MsG0580025527.01 | 0.808829 | 2.555769e-50 | 1.476306e-47 |
| MsG0380015572.01 | MsG0580025527.01 | 0.808113 | 3.632768e-50 | 2.059436e-47 |
| MsG0380015752.01 | MsG0580025527.01 | -0.809613 | 1.734997e-50 | 1.023520e-47 |
| MsG0380016839.01 | MsG0580025527.01 | 0.806625 | 7.512429e-50 | 4.096812e-47 |
| MsG0380016935.01 | MsG0580025527.01 | -0.811173 | 7.991482e-51 | 4.912253e-48 |
| MsG0380017405.01 | MsG0580025527.01 | -0.813493 | 2.489899e-51 | 1.628517e-48 |
| MsG0380017690.01 | MsG0580025527.01 | 0.818945 | 1.503518e-52 | 1.141462e-49 |
| MsG0380017812.01 | MsG0580025527.01 | -0.800182 | 1.624810e-48 | 7.516513e-46 |
| MsG0480018201.01 | MsG0580025527.01 | -0.800184 | 1.623360e-48 | 7.510119e-46 |
| MsG0280006883.01 | MsG0580025527.01 | 0.806001 | 1.016751e-49 | 5.457753e-47 |
| MsG0280007216.01 | MsG0580025527.01 | 0.820573 | 6.385924e-53 | 5.071721e-50 |
| MsG0280007759.01 | MsG0580025527.01 | 0.811618 | 6.398221e-51 | 3.979174e-48 |
| MsG0280008405.01 | MsG0580025527.01 | 0.836167 | 1.096514e-56 | 1.370751e-53 |
| MsG0280009167.01 | MsG0580025527.01 | 0.822766 | 1.987394e-53 | 1.679153e-50 |
| MsG0280009742.01 | MsG0580025527.01 | -0.808481 | 3.031791e-50 | 1.735536e-47 |
| MsG0280009864.01 | MsG0580025527.01 | -0.808991 | 2.359090e-50 | 1.368634e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025527.01.T01 | MTR_5g030420 | 71.429 | 238 | 54 | 5 | 1 | 227 | 1 | 235 | 5.65e-112 | 321 |
| MsG0580025527.01.T01 | MTR_5g030420 | 71.368 | 234 | 53 | 5 | 1 | 223 | 1 | 231 | 1.19e-109 | 316 |
| MsG0580025527.01.T01 | MTR_5g030430 | 72.872 | 188 | 47 | 3 | 23 | 209 | 40 | 224 | 4.32e-89 | 276 |
| MsG0580025527.01.T01 | MTR_8g067280 | 58.247 | 194 | 71 | 6 | 23 | 212 | 49 | 236 | 1.87e-67 | 219 |
| MsG0580025527.01.T01 | MTR_8g024790 | 32.512 | 203 | 98 | 5 | 17 | 215 | 19 | 186 | 1.64e-29 | 114 |
| MsG0580025527.01.T01 | MTR_8g024770 | 29.609 | 179 | 81 | 3 | 41 | 215 | 36 | 173 | 2.07e-23 | 97.8 |
| MsG0580025527.01.T01 | MTR_8g027495 | 32.653 | 196 | 122 | 5 | 28 | 215 | 53 | 246 | 3.74e-22 | 94.7 |
| MsG0580025527.01.T01 | MTR_1g072320 | 33.846 | 195 | 110 | 7 | 28 | 215 | 11 | 193 | 8.94e-22 | 93.6 |
| MsG0580025527.01.T01 | MTR_8g098275 | 32.164 | 171 | 102 | 5 | 43 | 207 | 19 | 181 | 3.64e-21 | 92.0 |
| MsG0580025527.01.T01 | MTR_7g117670 | 27.228 | 202 | 124 | 5 | 27 | 210 | 49 | 245 | 2.92e-14 | 71.6 |
| MsG0580025527.01.T01 | MTR_7g096350 | 27.895 | 190 | 119 | 6 | 27 | 210 | 49 | 226 | 9.43e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025527.01.T01 | AT4G17880 | 53.333 | 210 | 82 | 4 | 28 | 230 | 63 | 263 | 1.38e-66 | 216 |
| MsG0580025527.01.T01 | AT1G32640 | 55.440 | 193 | 82 | 3 | 23 | 212 | 63 | 254 | 8.19e-66 | 214 |
| MsG0580025527.01.T01 | AT5G46760 | 58.421 | 190 | 68 | 4 | 23 | 206 | 47 | 231 | 7.19e-60 | 198 |
| MsG0580025527.01.T01 | AT5G46830 | 43.478 | 207 | 106 | 5 | 14 | 210 | 20 | 225 | 1.07e-41 | 148 |
| MsG0580025527.01.T01 | AT4G09820 | 33.854 | 192 | 116 | 6 | 26 | 210 | 14 | 201 | 3.43e-26 | 106 |
| MsG0580025527.01.T01 | AT4G00870 | 33.163 | 196 | 101 | 3 | 27 | 221 | 34 | 200 | 9.14e-26 | 104 |
| MsG0580025527.01.T01 | AT1G01260 | 34.211 | 190 | 117 | 4 | 28 | 210 | 48 | 236 | 4.72e-25 | 103 |
| MsG0580025527.01.T01 | AT1G01260 | 34.211 | 190 | 117 | 4 | 28 | 210 | 48 | 236 | 4.72e-25 | 103 |
| MsG0580025527.01.T01 | AT1G01260 | 34.211 | 190 | 117 | 4 | 28 | 210 | 48 | 236 | 4.72e-25 | 103 |
| MsG0580025527.01.T01 | AT2G46510 | 34.536 | 194 | 116 | 4 | 27 | 210 | 47 | 239 | 3.17e-23 | 97.8 |
| MsG0580025527.01.T01 | AT1G63650 | 34.783 | 184 | 103 | 6 | 40 | 210 | 23 | 202 | 2.27e-21 | 92.4 |
| MsG0580025527.01.T01 | AT1G63650 | 34.783 | 184 | 103 | 6 | 40 | 210 | 23 | 202 | 2.27e-21 | 92.4 |
| MsG0580025527.01.T01 | AT1G63650 | 34.783 | 184 | 103 | 6 | 40 | 210 | 23 | 202 | 2.27e-21 | 92.4 |
| MsG0580025527.01.T01 | AT4G00480 | 31.905 | 210 | 113 | 7 | 20 | 207 | 13 | 214 | 2.85e-19 | 86.7 |
| MsG0580025527.01.T01 | AT4G00480 | 31.905 | 210 | 113 | 7 | 20 | 207 | 13 | 214 | 3.41e-19 | 86.3 |
| MsG0580025527.01.T01 | AT4G00480 | 31.905 | 210 | 113 | 7 | 20 | 207 | 13 | 214 | 3.88e-19 | 85.9 |
| MsG0580025527.01.T01 | AT5G41315 | 31.383 | 188 | 107 | 7 | 40 | 210 | 24 | 206 | 9.72e-19 | 85.1 |
| MsG0580025527.01.T01 | AT5G41315 | 30.952 | 210 | 121 | 8 | 20 | 210 | 10 | 214 | 9.95e-19 | 85.1 |
| MsG0580025527.01.T01 | AT5G41315 | 30.952 | 210 | 121 | 8 | 20 | 210 | 7 | 211 | 1.00e-18 | 85.1 |
Find 54 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCCCTTCTCTTCTCTTCT+CGG | 0.273706 | 5:+20009000 | MsG0580025527.01.T01:CDS |
| CTTCTCTTCTCTTCTCGGTT+GGG | 0.288232 | 5:+20009005 | MsG0580025527.01.T01:CDS |
| GTAAAGCTTGAAGTCTTTGT+TGG | 0.329221 | 5:-20008921 | None:intergenic |
| TTTCTACGGTGCTCTTGTTC+AGG | 0.338108 | 5:-20009096 | None:intergenic |
| GTCAACGACGATGGTCTTCC+TGG | 0.345860 | 5:+20009240 | MsG0580025527.01.T01:CDS |
| CCTTCTCTTCTCTTCTCGGT+TGG | 0.352582 | 5:+20009004 | MsG0580025527.01.T01:CDS |
| TTCTCTTCTCTTCTCGGTTG+GGG | 0.387275 | 5:+20009006 | MsG0580025527.01.T01:CDS |
| GAACTTAATTCTTTAATATC+CGG | 0.395031 | 5:+20009129 | MsG0580025527.01.T01:CDS |
| CCTTGCTTGAATCTTGTGGC+TGG | 0.403624 | 5:-20008892 | None:intergenic |
| TCATCAAGGTGAGAATGTTA+TGG | 0.406967 | 5:+20009494 | MsG0580025527.01.T01:CDS |
| TTGAATCTTGTGGCTGGTTG+TGG | 0.414616 | 5:-20008886 | None:intergenic |
| CATCAAGGTGAGAATGTTAT+GGG | 0.420008 | 5:+20009495 | MsG0580025527.01.T01:CDS |
| TTGCCCTCTCGCACCGAGAG+AGG | 0.425827 | 5:-20009317 | None:intergenic |
| AGACTTCAAGCTTTACTTGA+AGG | 0.440135 | 5:+20008928 | MsG0580025527.01.T01:CDS |
| TCTCTTCTCGGTTGGGGCGA+CGG | 0.441784 | 5:+20009012 | MsG0580025527.01.T01:CDS |
| TTACGCCATCATGTGGCAAC+AGG | 0.454661 | 5:+20008969 | MsG0580025527.01.T01:CDS |
| ATGAGAATCTTGCCCTCTCT+CGG | 0.500741 | 5:+20009304 | MsG0580025527.01.T01:CDS |
| TGGGGATCTTACCCACGTTA+AGG | 0.508650 | 5:+20008839 | MsG0580025527.01.T01:CDS |
| CCAACCGAGAAGAGAAGAGA+AGG | 0.508946 | 5:-20009004 | None:intergenic |
| GACGCTAGTGTGTATACCTT+CGG | 0.511322 | 5:+20009365 | MsG0580025527.01.T01:CDS |
| AATCTTGTGGCTGGTTGTGG+TGG | 0.514846 | 5:-20008883 | None:intergenic |
| TGCCCTCTCGCACCGAGAGA+GGG | 0.520582 | 5:-20009316 | None:intergenic |
| CCACATGATGGCGTAAGTCC+AGG | 0.522113 | 5:-20008962 | None:intergenic |
| TGCCCTCTCTCGGTGCGAGA+GGG | 0.529925 | 5:+20009314 | MsG0580025527.01.T01:CDS |
| AGTGCCTTGCTTGAATCTTG+TGG | 0.532619 | 5:-20008896 | None:intergenic |
| GTGTGTATACCTTCGGTGAA+TGG | 0.533941 | 5:+20009372 | MsG0580025527.01.T01:CDS |
| TTGCCCTCTCTCGGTGCGAG+AGG | 0.536595 | 5:+20009313 | MsG0580025527.01.T01:CDS |
| GTGAGAATGTTATGGGGACA+TGG | 0.543142 | 5:+20009502 | MsG0580025527.01.T01:CDS |
| GAGTTAAAATAAGCTTGTCC+AGG | 0.544735 | 5:-20009258 | None:intergenic |
| CCGCGCCGGTGGCCTTAACG+TGG | 0.545805 | 5:-20008851 | None:intergenic |
| TCTTCGCAGGTGGCTCATCA+AGG | 0.547052 | 5:+20009480 | MsG0580025527.01.T01:CDS |
| TAACTCAACTCCGGTGTGGT+TGG | 0.547738 | 5:+20009275 | MsG0580025527.01.T01:CDS |
| TTGAAGGTTCAAATGATACC+TGG | 0.552801 | 5:+20008944 | MsG0580025527.01.T01:CDS |
| GTTAGCCTGTTGCCACATGA+TGG | 0.567871 | 5:-20008974 | None:intergenic |
| TTCGTCGGAAACTGAGTCAC+CGG | 0.569158 | 5:-20009148 | None:intergenic |
| CAACCGAGAAGAGAAGAGAA+GGG | 0.569996 | 5:-20009003 | None:intergenic |
| GGTTGAGATAGCCGCGCCGG+TGG | 0.573986 | 5:-20008862 | None:intergenic |
| ATCAAATGCAGAGGATTCGT+CGG | 0.576494 | 5:-20009163 | None:intergenic |
| TCAACTCCGGTGTGGTTGGT+CGG | 0.585915 | 5:+20009279 | MsG0580025527.01.T01:CDS |
| CAAAGAAGACAAAGAAAACG+TGG | 0.598159 | 5:+20009056 | MsG0580025527.01.T01:CDS |
| CTTACCCACGTTAAGGCCAC+CGG | 0.601426 | 5:+20008846 | MsG0580025527.01.T01:CDS |
| CGGTGCGAGAGGGCAAGACA+AGG | 0.621590 | 5:+20009324 | MsG0580025527.01.T01:CDS |
| TGAGAATGTTATGGGGACAT+GGG | 0.629784 | 5:+20009503 | MsG0580025527.01.T01:CDS |
| CCTGGACTTACGCCATCATG+TGG | 0.635654 | 5:+20008962 | MsG0580025527.01.T01:CDS |
| CGCGCCGGTGGCCTTAACGT+GGG | 0.652073 | 5:-20008850 | None:intergenic |
| TCTAAAACACCATTCACCGA+AGG | 0.658540 | 5:-20009381 | None:intergenic |
| GGTGGTTGAGATAGCCGCGC+CGG | 0.668000 | 5:-20008865 | None:intergenic |
| TGGGGCGACGGTTACTACAA+CGG | 0.670140 | 5:+20009024 | MsG0580025527.01.T01:CDS |
| GCAAGACAAGGTCAAGAACA+CGG | 0.675201 | 5:+20009336 | MsG0580025527.01.T01:CDS |
| CCAGCCACAAGATTCAAGCA+AGG | 0.692151 | 5:+20008892 | MsG0580025527.01.T01:CDS |
| TCATTGCCGACCAACCACAC+CGG | 0.699028 | 5:-20009285 | None:intergenic |
| CCACGTTAAGGCCACCGGCG+CGG | 0.701282 | 5:+20008851 | MsG0580025527.01.T01:CDS |
| AAAGAAGACAAAGAAAACGT+GGG | 0.705479 | 5:+20009057 | MsG0580025527.01.T01:CDS |
| ATCAAGGTGAGAATGTTATG+GGG | 0.710516 | 5:+20009496 | MsG0580025527.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTTTGATAAATCAATT+CGG | - | Chr5:20009418-20009437 | None:intergenic | 15.0% |
| !! | GAACTTAATTCTTTAATATC+CGG | + | Chr5:20009129-20009148 | MsG0580025527.01.T01:CDS | 20.0% |
| !! | GTTCATTAAGAACTTTTCTA+CGG | - | Chr5:20009113-20009132 | None:intergenic | 25.0% |
| !!! | GTGAAGATGTTTTTTGATTT+TGG | + | Chr5:20009453-20009472 | MsG0580025527.01.T01:CDS | 25.0% |
| AAAAAGTTAATGACACTGAG+TGG | + | Chr5:20009187-20009206 | MsG0580025527.01.T01:CDS | 30.0% | |
| AAAGAAGACAAAGAAAACGT+GGG | + | Chr5:20009057-20009076 | MsG0580025527.01.T01:CDS | 30.0% | |
| !!! | AACTTTTTCATCAAATGCAG+AGG | - | Chr5:20009175-20009194 | None:intergenic | 30.0% |
| !!! | AGCTTATTTTAACTCAACTC+CGG | + | Chr5:20009266-20009285 | MsG0580025527.01.T01:CDS | 30.0% |
| !!! | GTGGTTCTTTTTAGTTTCTA+TGG | + | Chr5:20009206-20009225 | MsG0580025527.01.T01:CDS | 30.0% |
| AGACTTCAAGCTTTACTTGA+AGG | + | Chr5:20008928-20008947 | MsG0580025527.01.T01:CDS | 35.0% | |
| ATCAAGGTGAGAATGTTATG+GGG | + | Chr5:20009496-20009515 | MsG0580025527.01.T01:CDS | 35.0% | |
| CAAAGAAGACAAAGAAAACG+TGG | + | Chr5:20009056-20009075 | MsG0580025527.01.T01:CDS | 35.0% | |
| CATCAAGGTGAGAATGTTAT+GGG | + | Chr5:20009495-20009514 | MsG0580025527.01.T01:CDS | 35.0% | |
| GAGTTAAAATAAGCTTGTCC+AGG | - | Chr5:20009261-20009280 | None:intergenic | 35.0% | |
| GTAAAGCTTGAAGTCTTTGT+TGG | - | Chr5:20008924-20008943 | None:intergenic | 35.0% | |
| TCATCAAGGTGAGAATGTTA+TGG | + | Chr5:20009494-20009513 | MsG0580025527.01.T01:CDS | 35.0% | |
| TTGAAGGTTCAAATGATACC+TGG | + | Chr5:20008944-20008963 | MsG0580025527.01.T01:CDS | 35.0% | |
| ATCAAATGCAGAGGATTCGT+CGG | - | Chr5:20009166-20009185 | None:intergenic | 40.0% | |
| TGAGAATGTTATGGGGACAT+GGG | + | Chr5:20009503-20009522 | MsG0580025527.01.T01:CDS | 40.0% | |
| ! | TCTAAAACACCATTCACCGA+AGG | - | Chr5:20009384-20009403 | None:intergenic | 40.0% |
| ! | TGATTTTGGATCTTCTTCGC+AGG | + | Chr5:20009467-20009486 | MsG0580025527.01.T01:CDS | 40.0% |
| !!! | ATTTTAACTCAACTCCGGTG+TGG | + | Chr5:20009271-20009290 | MsG0580025527.01.T01:CDS | 40.0% |
| !!! | GTGAATGGTGTTTTAGAGCT+TGG | + | Chr5:20009387-20009406 | MsG0580025527.01.T01:CDS | 40.0% |
| AGTGCCTTGCTTGAATCTTG+TGG | - | Chr5:20008899-20008918 | None:intergenic | 45.0% | |
| ATGAGAATCTTGCCCTCTCT+CGG | + | Chr5:20009304-20009323 | MsG0580025527.01.T01:CDS | 45.0% | |
| CAACCGAGAAGAGAAGAGAA+GGG | - | Chr5:20009006-20009025 | None:intergenic | 45.0% | |
| CTTCTCTTCTCTTCTCGGTT+GGG | + | Chr5:20009005-20009024 | MsG0580025527.01.T01:CDS | 45.0% | |
| GACGCTAGTGTGTATACCTT+CGG | + | Chr5:20009365-20009384 | MsG0580025527.01.T01:CDS | 45.0% | |
| GCAAGACAAGGTCAAGAACA+CGG | + | Chr5:20009336-20009355 | MsG0580025527.01.T01:CDS | 45.0% | |
| GTGAGAATGTTATGGGGACA+TGG | + | Chr5:20009502-20009521 | MsG0580025527.01.T01:CDS | 45.0% | |
| GTGTGTATACCTTCGGTGAA+TGG | + | Chr5:20009372-20009391 | MsG0580025527.01.T01:CDS | 45.0% | |
| TCTCCCTTCTCTTCTCTTCT+CGG | + | Chr5:20009000-20009019 | MsG0580025527.01.T01:CDS | 45.0% | |
| TTCTCTTCTCTTCTCGGTTG+GGG | + | Chr5:20009006-20009025 | MsG0580025527.01.T01:CDS | 45.0% | |
| TTTCTACGGTGCTCTTGTTC+AGG | - | Chr5:20009099-20009118 | None:intergenic | 45.0% | |
| ! | CAGTCTTTTGTCAACGACGA+TGG | + | Chr5:20009231-20009250 | MsG0580025527.01.T01:CDS | 45.0% |
| ! | TTGAATCTTGTGGCTGGTTG+TGG | - | Chr5:20008889-20008908 | None:intergenic | 45.0% |
| ! | TTTTGGATCTTCTTCGCAGG+TGG | + | Chr5:20009470-20009489 | MsG0580025527.01.T01:CDS | 45.0% |
| CCAACCGAGAAGAGAAGAGA+AGG | - | Chr5:20009007-20009026 | None:intergenic | 50.0% | |
| CCAGCCACAAGATTCAAGCA+AGG | + | Chr5:20008892-20008911 | MsG0580025527.01.T01:CDS | 50.0% | |
| CCTTCTCTTCTCTTCTCGGT+TGG | + | Chr5:20009004-20009023 | MsG0580025527.01.T01:CDS | 50.0% | |
| CCTTGCTTGAATCTTGTGGC+TGG | - | Chr5:20008895-20008914 | None:intergenic | 50.0% | |
| TAACTCAACTCCGGTGTGGT+TGG | + | Chr5:20009275-20009294 | MsG0580025527.01.T01:CDS | 50.0% | |
| TGGGGATCTTACCCACGTTA+AGG | + | Chr5:20008839-20008858 | MsG0580025527.01.T01:CDS | 50.0% | |
| TTACGCCATCATGTGGCAAC+AGG | + | Chr5:20008969-20008988 | MsG0580025527.01.T01:CDS | 50.0% | |
| TTCGTCGGAAACTGAGTCAC+CGG | - | Chr5:20009151-20009170 | None:intergenic | 50.0% | |
| ! | AATCTTGTGGCTGGTTGTGG+TGG | - | Chr5:20008886-20008905 | None:intergenic | 50.0% |
| ! | GTTAGCCTGTTGCCACATGA+TGG | - | Chr5:20008977-20008996 | None:intergenic | 50.0% |
| CCTGGACTTACGCCATCATG+TGG | + | Chr5:20008962-20008981 | MsG0580025527.01.T01:CDS | 55.0% | |
| CTTACCCACGTTAAGGCCAC+CGG | + | Chr5:20008846-20008865 | MsG0580025527.01.T01:CDS | 55.0% | |
| GTCAACGACGATGGTCTTCC+TGG | + | Chr5:20009240-20009259 | MsG0580025527.01.T01:CDS | 55.0% | |
| TCAACTCCGGTGTGGTTGGT+CGG | + | Chr5:20009279-20009298 | MsG0580025527.01.T01:CDS | 55.0% | |
| TCATTGCCGACCAACCACAC+CGG | - | Chr5:20009288-20009307 | None:intergenic | 55.0% | |
| TCTTCGCAGGTGGCTCATCA+AGG | + | Chr5:20009480-20009499 | MsG0580025527.01.T01:CDS | 55.0% | |
| TGGGGCGACGGTTACTACAA+CGG | + | Chr5:20009024-20009043 | MsG0580025527.01.T01:CDS | 55.0% | |
| ! | CCACATGATGGCGTAAGTCC+AGG | - | Chr5:20008965-20008984 | None:intergenic | 55.0% |
| TCTCTTCTCGGTTGGGGCGA+CGG | + | Chr5:20009012-20009031 | MsG0580025527.01.T01:CDS | 60.0% | |
| CGGTGCGAGAGGGCAAGACA+AGG | + | Chr5:20009324-20009343 | MsG0580025527.01.T01:CDS | 65.0% | |
| GGTGGTTGAGATAGCCGCGC+CGG | - | Chr5:20008868-20008887 | None:intergenic | 65.0% | |
| TGCCCTCTCGCACCGAGAGA+GGG | - | Chr5:20009319-20009338 | None:intergenic | 65.0% | |
| TTGCCCTCTCGCACCGAGAG+AGG | - | Chr5:20009320-20009339 | None:intergenic | 65.0% | |
| ! | TGCCCTCTCTCGGTGCGAGA+GGG | + | Chr5:20009314-20009333 | MsG0580025527.01.T01:CDS | 65.0% |
| ! | TTGCCCTCTCTCGGTGCGAG+AGG | + | Chr5:20009313-20009332 | MsG0580025527.01.T01:CDS | 65.0% |
| CCACGTTAAGGCCACCGGCG+CGG | + | Chr5:20008851-20008870 | MsG0580025527.01.T01:CDS | 70.0% | |
| GGTTGAGATAGCCGCGCCGG+TGG | - | Chr5:20008865-20008884 | None:intergenic | 70.0% | |
| ! | CGCGCCGGTGGCCTTAACGT+GGG | - | Chr5:20008853-20008872 | None:intergenic | 70.0% |
| ! | CCGCGCCGGTGGCCTTAACG+TGG | - | Chr5:20008854-20008873 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20008838 | 20009533 | 20008838 | ID=MsG0580025527.01;Name=MsG0580025527.01 |
| Chr5 | mRNA | 20008838 | 20009533 | 20008838 | ID=MsG0580025527.01.T01;Parent=MsG0580025527.01;Name=MsG0580025527.01.T01;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|231 |
| Chr5 | exon | 20008838 | 20009533 | 20008838 | ID=MsG0580025527.01.T01:exon:8396;Parent=MsG0580025527.01.T01 |
| Chr5 | CDS | 20008838 | 20009533 | 20008838 | ID=MsG0580025527.01.T01:cds;Parent=MsG0580025527.01.T01 |
| Gene Sequence |
| Protein sequence |
