AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029444.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029444.01.T01 MTR_5g086320 94.304 158 9 0 1 158 1 158 1.92e-105 298
MsG0580029444.01.T01 MTR_7g102860 71.681 113 30 2 46 157 192 303 9.10e-48 157
MsG0580029444.01.T01 MTR_0014s0040 65.714 70 22 2 48 116 72 140 2.23e-21 85.5
MsG0580029444.01.T01 MTR_0014s0040 65.714 70 22 2 48 116 125 193 8.46e-21 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAATATATTGATTTAG+AGG 0.307351 5:-97031977 MsG0580029444.01.T01:CDS
TAGTGTTGTTACTATGCTAT+TGG 0.309426 5:-97032019 MsG0580029444.01.T01:CDS
AGTGTTGTTACTATGCTATT+GGG 0.375847 5:-97032018 MsG0580029444.01.T01:CDS
TACGTTATAAAGCAATCAAT+TGG 0.401927 5:-97031744 MsG0580029444.01.T01:CDS
TCTTCCATTGATTAAGAAGA+AGG 0.407603 5:-97031683 MsG0580029444.01.T01:CDS
AAATCCTTCTTCTTAATCAA+TGG 0.410446 5:+97031679 None:intergenic
TTGAGAATCGCCAATCTGTT+GGG 0.465643 5:+97031709 None:intergenic
TTTGAGAATCGCCAATCTGT+TGG 0.477273 5:+97031708 None:intergenic
TAAGAAGAAGGATTTCCTCT+TGG 0.487475 5:-97031671 MsG0580029444.01.T01:CDS
TGCTATTGGGTGCAATAGCT+TGG 0.489850 5:-97032005 MsG0580029444.01.T01:CDS
GACTGAAAATCCCAACAGAT+TGG 0.525742 5:-97031719 MsG0580029444.01.T01:CDS
CAATTCTATTATGAGAGACA+TGG 0.595369 5:-97031884 MsG0580029444.01.T01:CDS
AAAACGTAAAAGAAATGAAG+AGG 0.599015 5:-97032076 MsG0580029444.01.T01:CDS
TGAAGAGGTGGCAGAGCTCG+AGG 0.600263 5:-97032061 MsG0580029444.01.T01:CDS
CTAAGAAGAGAGACGAAGAG+AGG 0.631658 5:-97031912 MsG0580029444.01.T01:CDS
ACGTAAAAGAAATGAAGAGG+TGG 0.634856 5:-97032073 MsG0580029444.01.T01:CDS
CTCAAGACATAGAAGCCAAG+AGG 0.672040 5:+97031656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTACAAGTTACTAAAAAA+AGG - Chr5:97031925-97031944 MsG0580029444.01.T01:CDS 20.0%
!!! CAACAAATATATTGATTTAG+AGG - Chr5:97031791-97031810 MsG0580029444.01.T01:CDS 20.0%
!!! TTTTTTTAGTAACTTGTAGA+AGG + Chr5:97031926-97031945 None:intergenic 20.0%
! AAAACGTAAAAGAAATGAAG+AGG - Chr5:97031692-97031711 MsG0580029444.01.T01:CDS 25.0%
! AAATCCTTCTTCTTAATCAA+TGG + Chr5:97032092-97032111 None:intergenic 25.0%
! ACAAGTTACTAAAAAAAGGA+TGG - Chr5:97031929-97031948 MsG0580029444.01.T01:CDS 25.0%
! TACGTTATAAAGCAATCAAT+TGG - Chr5:97032024-97032043 MsG0580029444.01.T01:CDS 25.0%
!!! TAGTACTTTTTTCTTTGTTG+CGG + Chr5:97031826-97031845 None:intergenic 25.0%
CAATTCTATTATGAGAGACA+TGG - Chr5:97031884-97031903 MsG0580029444.01.T01:CDS 30.0%
GTTACTAAAAAAAGGATGGA+AGG - Chr5:97031933-97031952 MsG0580029444.01.T01:CDS 30.0%
! AGTGTTGTTACTATGCTATT+GGG - Chr5:97031750-97031769 MsG0580029444.01.T01:CDS 30.0%
! TAGTGTTGTTACTATGCTAT+TGG - Chr5:97031749-97031768 MsG0580029444.01.T01:CDS 30.0%
! TCTTCCATTGATTAAGAAGA+AGG - Chr5:97032085-97032104 MsG0580029444.01.T01:CDS 30.0%
!! GGAAGGAAATTTTCAAGAAA+TGG - Chr5:97031950-97031969 MsG0580029444.01.T01:CDS 30.0%
!!! CGTTTTCTTGAAAGTATTGA+AGG + Chr5:97031678-97031697 None:intergenic 30.0%
ACGTAAAAGAAATGAAGAGG+TGG - Chr5:97031695-97031714 MsG0580029444.01.T01:CDS 35.0%
TAAGAAGAAGGATTTCCTCT+TGG - Chr5:97032097-97032116 MsG0580029444.01.T01:CDS 35.0%
GACTGAAAATCCCAACAGAT+TGG - Chr5:97032049-97032068 MsG0580029444.01.T01:CDS 40.0%
TTGAGAATCGCCAATCTGTT+GGG + Chr5:97032062-97032081 None:intergenic 40.0%
TTTGAGAATCGCCAATCTGT+TGG + Chr5:97032063-97032082 None:intergenic 40.0%
! TTTTTCTTTGTTGCGGAGCT+TGG + Chr5:97031819-97031838 None:intergenic 40.0%
CTAAGAAGAGAGACGAAGAG+AGG - Chr5:97031856-97031875 MsG0580029444.01.T01:CDS 45.0%
TGCTATTGGGTGCAATAGCT+TGG - Chr5:97031763-97031782 MsG0580029444.01.T01:CDS 45.0%
TGAAGAGGTGGCAGAGCTCG+AGG - Chr5:97031707-97031726 MsG0580029444.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 97031657 97032133 97031657 ID=MsG0580029444.01;Name=MsG0580029444.01
Chr5 mRNA 97031657 97032133 97031657 ID=MsG0580029444.01.T01;Parent=MsG0580029444.01;Name=MsG0580029444.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|158
Chr5 exon 97031657 97032133 97031657 ID=MsG0580029444.01.T01:exon:24837;Parent=MsG0580029444.01.T01
Chr5 CDS 97031657 97032133 97031657 ID=MsG0580029444.01.T01:cds;Parent=MsG0580029444.01.T01
Gene Sequence

>MsG0580029444.01.T01

ATGGGTAGTAGTGTAGATCCTTCAATACTTTCAAGAAAACGTAAAAGAAATGAAGAGGTGGCAGAGCTCGAGGAAACAATCTTCATTGTTTGTAGTGTTGTTACTATGCTATTGGGTGCAATAGCTTGGTATCACAACAAATATATTGATTTAGAGGAACCAAGCTCCGCAACAAAGAAAAAAGTACTATGTACTCGTGCTAAGAAGAGAGACGAAGAGAGGATAATCAATTCTATTATGAGAGACATGGCTGAGTCATTGAAAGACCTTCTACAAGTTACTAAAAAAAGGATGGAAGGAAATTTTCAAGAAATGGTGCAAGAAGTGTTGAATGAAATGAAGATGATTATTGATATTGATGATACTTTACGTTATAAAGCAATCAATTGGTTGACTGAAAATCCCAACAGATTGGCGATTCTCAAAGCTCTTCCATTGATTAAGAAGAAGGATTTCCTCTTGGCTTCTATGTCTTGA

Protein sequence

>MsG0580029444.01.T01

MGSSVDPSILSRKRKRNEEVAELEETIFIVCSVVTMLLGAIAWYHNKYIDLEEPSSATKKKVLCTRAKKRDEERIINSIMRDMAESLKDLLQVTKKRMEGNFQEMVQEVLNEMKMIIDIDDTLRYKAINWLTENPNRLAILKALPLIKKKDFLLASMS*